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Analiza trwałości zmęczeniowej asfaltów drogowych i modyfikowanych z wykorzystaniem testu liniowego zwiększania amplitudy / Analysis of the fatigue life of neat and modified bitumens using linear amplitude sweep test
PublikacjaThe paper presents an evaluation of the fatigue resistance of selected Polish bituminous binders: neat bitumen 50/70, polymer modified bitumen PMB 45/80-55 and polymer highly modified bitumen PMB 45/80-80. The linear amplitude sweep (LAS) test, which is a new bitumen performance grading test developed in the USA, and was used to assess the fatigue life of the bitumens. Highly modified bitumen 45/80-80 showed 10-20 times higher...
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DIMEO ÁLVAREZ, Carlos, WENDORF, Anna (coord.), Reflexiones sobre la literatura venezolana. Un estudio monográfico. Bielsko-Biała: ATH Akademia Techniczno-Humanistyczna, University of Bielsko-Biała, 2015. 221 pp. ISBN: 978-83-65182-28-9
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Development and performance analysis of a novel multiphase doubly-fed induction generator
PublikacjaThis paper presents the research into the design and performance analysis of a novel five-phase doubly-fed induction generator (DFIG). The designed DFIG is developed based on standard induction motor components and equipped with a five-phase rotor winding supplied from the five-phase inverter. This approach allows the machine to be both efficient and reliable due to the ability of the five-phase rotor winding to operate during...
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Checkpoints that regulate balanced biosynthesis of lipopolysaccharide and its essentiality in Escherichia coli
PublikacjaThe outer membrane (OM) of Gram-negative bacteria, such as Escherichia coli, is essential for their viability. Lipopolysaccharide (LPS) constitutes the major component of OM, providing the permeability barrier, and a tight balance exists between LPS and phospholipids amounts as both of these essential components use a common metabolic precursor. Hence, checkpoints are in place, right from the regulation of the first committed step...
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Towards an analysis framework for operational risk coupling mode: A case from MASS navigating in restricted waters
PublikacjaMaritime Autonomous Surface Ships (MASSs) constitute highly interconnected and tightly coupled multistate systems. Incorporating the coupling effects of both interactions and dependencies is centrally important to ensure navigation safety of MASSs. This paper proposes a framework for examining the coupling effects in the operational modes (OM) of MASSs. Failure Modes (FMs) of MASSs related to interactions with the environment and...
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Polyurethanes as a Potential Medical-Grade Filament for Use in Fused Deposition Modeling 3D Printers – a Brief Review
PublikacjaThe possibility of using 3D printing technology (3DP) in medical field is a kind of revolution in health care. This has contributed to a rapid growth in demand for 3D printers, whose systems and materials are adapted to strict medical requirements. In this paper, we report a brief review of polyurethanes as a potential medical-grade filament for use in Fused Deposition Modeling (FDM) 3D printer technology. The advantages of polyurethanes...
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Karaś P., Kochanowicz K., Pitek M., Domański P., Obuchowski I., Tomiczek B., Liberek K.: Evolution towards simplicity in bacterial small heat shock protein system// eLife -, (2023), s.1-21
PublikacjaEvolution can tinker with multi-protein machines and replace them with simpler single-protein systems performing equivalent functions in an equally efficient manner. It is unclear how, on a molecular level, such simplification can arise. With ancestral reconstruction and biochemical analysis, we have traced the evolution of bacterial small heat shock proteins (sHsp), which help to refold proteins from aggregates using either...
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Evolution towards simplicity in bacterial small heat shock protein system
PublikacjaEvolution can tinker with multi-protein machines and replace them with simpler single-protein systems performing equivalent functions in an equally efficient manner. It is unclear how, on a molecular level, such simplification can arise. With ancestral reconstruction and biochemical analysis, we have traced the evolution of bacterial small heat shock proteins (sHsp), which help to refold proteins from aggregates using either two...
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Characterization of Bioactivity of Selective Molecules in Fruit Wines by FTIR and NMR Spectroscopies, Fluorescence and Docking Calculations
PublikacjaFourier transform infrared (FTIR) and proton nuclear magnetic resonance (1H NMR) spectroscopies were applied to characterize and compare the chemical shifts in the polyphenols’ regions of some fruit wines. The obtained results showed that FTIR spectra (1800–900 cm−1) and 1H NMR (δ 6.5–9.3 ppm) of different fruit wines can be used as main indices of the year of vintage and quality of fruit wines. In addition to the classical determination...
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Mechanochemical Energy Transduction during the Main Rotary Step in the Synthesis Cycle of F1-ATPase
PublikacjaF1-ATPase is a highly efficient molecular motor that can synthesize ATP driven by a mechanical torque. Its ability to function reversibly in either direction requires tight mechanochemical coupling between the catalytic domain and the rotating central shaft, as well as temporal control of substrate binding and product release. Despite great efforts and significant progress, the molecular details of this synchronized and fine-tuned...
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Interfacial water controls the process of adsorption of hyperactive antifreeze proteins onto the ice surface
PublikacjaA mechanism of interactions between the ice-binding surface of a hyperactive antifreeze protein molecule and the ice surface is proposed, involving the influence of water present between the two surfaces on the behavior of the approaching molecule. It is demonstrated that the interfacial water, even before its full solidification, can act as a factor that pushes away or pulls nearer the protein molecule to ensure its proper positioning....
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A mobile loop order–disorder transition modulates the speed of chaperonin cycling
PublikacjaMolecular machines order and disorder polypeptides as they form and dissolve large intermolecular interfaces, but the biological significance of coupled ordering and binding has been established in few, if any, macromolecular systems. The ordering and binding of GroES co-chaperonin mobile loops accompany an ATP-dependent conformational change in the GroEL chaperonin that promotes client protein folding. Following ATP hydrolysis,...
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Structural, magnetic and spectral properties of tetrahedral cobalt(II) silanethiolates: a variety of structures and manifestation of field-induced slow magnetic relaxation
PublikacjaBlue crystals of five heteroleptic cobalt(II) silanethiolates 1–5 have been obtained by the reaction of [Co{SSi (tBuO)3}2(NH3)]2 with aminopyridines and aminomethylpyridines at an appropriate molar ratio and their structural, spectral, thermal and magnetic properties have been established and described. All complexes 1–5 contain Co(II) ions in a tetrahedral CoN2S2 environment formed by (tBuO)3SiS− residues and pyridines and present...
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Urban lighting masterplan – origins, definitions, methodologies and collaborations
PublikacjaAwareness of the significance and benefits of properly designed urban lighting masterplans has been growing since the early 21 st century. There are many factors driving this notable change, such as developments in lighting technology, energy conservation, city branding design and economics, environmental impacts, human health and wellbeing, and people-oriented sociological aspects. As the profession of ‘independent urban lighting...
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Scheduling with Complete Multipartite Incompatibility Graph on Parallel Machines
PublikacjaIn this paper we consider a problem of job scheduling on parallel machines with a presence of incompatibilities between jobs. The incompatibility relation can be modeled as a complete multipartite graph in which each edge denotes a pair of jobs that cannot be scheduled on the same machine. Our research stems from the works of Bodlaender, Jansen, and Woeginger (1994) and Bodlaender and Jansen (1993). In particular, we pursue the...
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Restrained differential of a graph
PublikacjaGiven a graph $G=(V(G), E(G))$ and a vertex $v\in V(G)$, the {open neighbourhood} of $v$ is defined to be $N(v)=\{u\in V(G) :\, uv\in E(G)\}$. The {external neighbourhood} of a set $S\subseteq V(G)$ is defined as $S_e=\left(\cup_{v\in S}N(v)\right)\setminus S$, while the \emph{restrained external neighbourhood} of $S$ is defined as $S_r=\{v\in S_e : N(v)\cap S_e\neq \varnothing\}$. The restrained differential of a graph $G$ is...
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Homoisocitrate dehydrogenase from Candida albicans: properties, inhibition, and targeting by an antifungal pro-drug
PublikacjaThe LYS12 gene from Candida albicans, coding for homoisocitrate dehydrogenase was cloned and expressed as a His-tagged protein in Escherichia coli. The purified gene product catalyzes the Mg2+- and K+-dependent oxidative decarboxylation of homoisocitrate to alpha-ketoadipate. The recombinant enzyme demonstrates strict specificity for homoisocitrate. SDS-PAGE of CaHIcDH revealed its molecular mass of 42.6±1 kDa, while in size-exclusion...
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Scheduling jobs to contain a natural disaster: a model and complexity
Publikacjathis paper is devoted to the problem of scheduling suppression units so that a natural disaster is dealt with as efficient as possible. the concept of deteriorating jobs is adopted, that is, the formal model of scheduling represents linearly increasing value loss as the disaster remains unsuppressed and increasing time for its suppression. more precisely, two different goals are considered: finding a suppression schedule of minimal...
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Photophysics of Soret-Excited Tin(IV) Porphyrins in Solution
PublikacjaThe photophysics of low-chlorin tin(IV) tetraphenylporphyrin dihydroxide, a core building block for axially substituted supramolecular tin porphyrin constructs, has been studied in a variety of hydrogen-bonding, nonpolar, and aprotic polar solvents using steady-state, nanosecond, and femtosecond time-resolved emission, and femtosecond time-resolved absorption methods. In hydrogen-bonding solvents the metalloporphyrin exists as...
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Sensorless control of five-phase induction machine supplied by the VSI with output filter
PublikacjaIn this paper, a novel sensorless control structure based on multi-scalar variables is proposed. The tatic feedback control law is obtained by using the multi-scalar variables transformation, where the multi-scalar variables approach allows a full linearization of the nonlinear system. The control system could be described as “optimized” because of the minimized number of controllers. Furthermore, control system is divided into...
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Determinants of judges’ career choices and productivity: a Polish case study
PublikacjaThe goal of this paper is to identify factors which affect judges’ productivity and career choice motives with the view of increasing judicial efficiency. Specifically, the investigation focuses on such aspects as judges’ remuneration, promotion, threat of judgment revocation, service/mission, periodic assessment, the threat of a complaint about protracted proceedings or of disciplinary proceedings, the threat of destabilization...
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Pomiar temperatury zeszklenia metodą zmiennotemperaturowej elipsometrii spektroskopowej
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Optyczna spektroskopia podczerwieni cienkich warstw FTIR - podstawy identyfikacji widm poliazometin
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Planowanie eksperymentu
PublikacjaW pracy przedstawiono schemat postępowania przy planowaniu eksperymentu naukowego
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Wiedza ogólna o eksperymencie naukowym
PublikacjaW pracy przedstawiono podstawowe pojęcia z zakresu metodologii badań w nauce i w technice
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RUSSIANS ON THE POLISH LABOUR MARKET
PublikacjaThe article looks into the employment of Russian citizens in Poland in 2004– 2018. It presents the legal basis for Russians’ entering Poland and taking up work without having to seek a work permit, and specifies who must apply for such a permit. Russian citizens can obtain refugee status under the Geneva Convention, which grants them the right to move freely, choose their place of residence and undertake paid employment, while...
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Metodyka wykonywania pomiarów oraz ocena niepewności i błędów pomiaru
Publikacjaelem każdego ćwiczenia w laboratorium studenckim jest zmierzenie pewnych wielkości, a następnie ob- liczenie na podstawie tych wyników pomiarów wartości wielkości badanej. Rezulta- tem końcowym badań jest nie tylko otrzymany wynik liczbowy. Nie mniej ważne jest dokonanie oceny dokład- ności pomiaru oraz opraco- wanie wniosków końcowych. Warto zadać sobie pytanie: czy to, co zostało zmierzone, ma sens i co z tego wynika?...
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Twitter-Based uncertainty and cryptocurrency returns
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Cation sensing by diphenyl-azobenzocrowns
PublikacjaMetal cations complexation and proton binding by 13- and 16-membered diphenyl-azobenzocrowns and diphenyl-hydroxyazobenzocrowns were studied in acetonitrile using spectroscopic methods: UV–vis spectroscopy, spectrofluorimetry, and 1H NMR spectroscopy. Phenyl moieties in benzene rings were found to affect binding strength alkali and alkaline earth metal cations and hydrogen ion, and affect tautomeric equilibrium of hydroxyazobenzocrowns....
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Marine traffic risk modelling – an innovative approach and a case study
PublikacjaThis paper presents a model to analyse the risk of two common marine accidents: collision and grounding. Attention is focused on oil tankers since they pose the highest environmental risks. A case study in selected areas of the Gulf of Finland in ice-free conditions is presented. The model utilizes a formula for risk calculation that considers both the probability of an unwanted event and its consequences. The model can be decomposed...
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New perspective on the in vivo use of cold stress dynamic thermography in integumental reconstruction with the use of skin-muscle flaps
PublikacjaAmong the problems encountered by plastic surgeons is the reconstruction of defects following tumors. One of the reconstructive options is TRAM flap. Despite that anatomy is well-explored, marginal flap necrosis may develop. To minimize complications imaging examinations was designed to determine the degree of flap perfusion. One of them is the thermographic examination.
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Unusual structural properties of water within the hydration shell of hyperactive antifreeze protein
PublikacjaMany hypotheses can be encountered explaining the mechanism of action of antifreeze proteins. One widespread theory postulates that the similarity of structural properties of solvation water of antifreeze proteins to ice is crucial to the antifreeze activity of these agents. In order to investigate this problem, the structural properties of solvation water of the hyperactive antifreeze protein from Choristoneura fumiferana were...
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Why do G-quadruplexes dimerize through the 5’-ends? Driving forces for G4 DNA dimerization examined in atomic detail
PublikacjaG-quadruplexes (G4) are secondary structures formed by guanine-rich nucleic acid sequences and shown to exist in living cells where they participate in regulation of gene expression and chromosome maintenance. G-quadruplexes with solvent-exposed guanine tetrads show the tendency to associate together through cofacial stacking, which may be important for packaging of G4-forming sequences and allows for the design of higher-order...
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The interactions of monomeric acridines and unsymmetrical bisacridines (UAs) with DNA duplexes: an insight provided by NMR and MD studies
PublikacjaMembers of a novel class of anticancer compounds, exhibiting high antitumor activity, i.e. the unsymmetrical bisacridines (UAs), consist of two heteroaromatic ring systems. One of the ring systems is an imidazoacridinone moiety, with the skeleton identical to the structural base of Symadex. The second one is a 1-nitroacridine moiety, hence it may be regarded as Nitracrine’s structural basis. These monoacridine units are connected...
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Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces
PublikacjaIn this work, we study the electrodes with a periodic matrix of gold particles pattered by titanium dimples and modified by 3-mercaptopropionic acid (MPA) followed by CD147 receptor grafting for specific impedimetric detection of SARS-CoV-2 viral spike proteins. The synergistic DFT and MM/MD modeling revealed that MPA adsorption geometries on the Au–Ti surface have preferential and stronger binding patterns through the carboxyl...
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The Impact of Long-Time Chemical Bonds in Mineral-Cement-Emulsion Mixtures on Stiffness Modulus
PublikacjaDeep cold in-place recycling is the most popular method of reuse of existing old and deteriorated asphalt layers of road pavements. In Poland, in most cases, the Mineral-Cement-Emulsion mixture technology is used, but there are also applications combining foamed bitumen and cement. Mineral-Cement-Emulsion mixtures contain two different binding agents – cement as well as asphalt from the asphalt emulsion. Asphalt creates asphalt...
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Cement kiln dust
PublikacjaThe volume of cement production in the world has remained at the level of approxi mately 4.1 billion tons/year. The cement production process is energy intensive and is the world’s leading emitter of carbon dioxide. Therefore, the main activities cement plants are aimed at introducing technologies changes in production of clinkier.The construction industry is responsible for the majority of CO2 emissions to the atmosphere: the...
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Polynomial Algorithm for Minimal (1,2)-Dominating Set in Networks
PublikacjaDominating sets find application in a variety of networks. A subset of nodes D is a (1,2)-dominating set in a graph G=(V,E) if every node not in D is adjacent to a node in D and is also at most a distance of 2 to another node from D. In networks, (1,2)-dominating sets have a higher fault tolerance and provide a higher reliability of services in case of failure. However, finding such the smallest set is NP-hard. In this paper, we...
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Bacteriophages as Factories for Eu2O3 Nanoparticle Synthesis
PublikacjaThe use of phage display to identify peptides with an ability to bind and synthesize Eu2O3 nanoparticles is demonstrated in this report. This is the first report of modified phages specifically binding a lanthanide. The peptides exposed on virions revealed very strong binding to Eu2O3 nanoparticles and the ability to catalyze Eu2O3 nanoparticles’ formation from Eu(OH)3 and Eu(NO3)3 solutions. The luminescence emission spectrum...
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Performance Evaluation of Asphalt Binder Modified with Shear Thickening Fluid
PublikacjaThis paper aims at using a dilatant or shear thickening fluid (STF) [a non-Newtonian fluid consisting of particles of nano-silica suspended in a liquid medium, i.e., ethylene glycol (EG), which acts as a carrier fluid; proportion 40:60] as additive or modifier of bitumen in order to enhance its viscoelastic properties. A commonly-used performance grading (PG) system, storage stability test, and different conventional and rheological...
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Modification of quaternary structure of Candida albicans GlcN-6-P synthase and its desensitization to inhibition by UDP-GlcNAc by site-directed mutagenesis
PublikacjaSite-directed mutagenesis of the CaGFA1 gene encoding glucosamine-6-phosphate synthase from Candida albicans was performed. Desensitization of the enzyme to inhibition by UDPGlcNAc was achieved upon T487I and H492F substitutions at the UDP-GlcNAc binding site, exchange of D524, S525 and S527 for Ala at the dimer:dimer interface and construction of the tail-lock array (L434R and L460A) at the C-tail region. The first two sets if...
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Characterization of metabolites in different kiwifruit varieties by NMR and fluorescence spectroscopy
PublikacjaIt is known from our previous studies that kiwifruits, which are used in common human diet, have preventive properties of coronary artery disease. This study describes a combination of 1H NMR spectroscopy, multivariate data analyses and fluorescence measurements in differentiating of some kiwifruit varieties, their quenching and antioxidant properties. A total of 41 metabolites were identified by comparing with literature data...
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Genetic Programming for Workload Balancing in the Comcute Grid System
PublikacjaA genetic programming paradigm is implemented for reliability optimization in the Comcute grid system design. Chromosomes are generated as the program functions and then genetic operators are applied for finding Pareto-suboptimal task assignment and scheduling. Results are compared with outcomes obtained by an adaptive evolutionary algorithm.
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Organic-inorganic composites consisted of poly(3,4-ethylenedioxythiophene)and Prussian Blue analogues
PublikacjaThe organic-inorganic material consisted of poly(3,4-ethylenedioxythiophene) (pEDOT) and copper hexacyanoferrate (Cuhcf) was synthesized. The pEDOT film with Fe(CN)63−/4− as counter-ions potentiodynamically polarized in aqueous CuCl2 electrolyte brings about stable hybrid material (pEDOT/Cuhcf) performing single redox activity of FeII/III at a formal potential Ef = 0.61 V (vs. Ag/AgCl/0.1 M KCl) and less clearly shaped two redox...
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Metaheuristic algorithms for optimization of resilient overlay computing systems
PublikacjaThe idea of distributed computing systems has been gaining much interest in recent years owing to the growing amount of data to be processed for both industrial and academic purposes. However, similar to other systems, also distributed computing systems are vulnerable to failures. Due to strict QoS requirements, survivability guarantees are necessary for provisioning of uninterrupted service. In this article, we focus on reliability...
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Novel anticancer strategy aimed at targeting shelterin complexes by the induction of structural changes in telomeric DNA: hitting two birds with one stone.
PublikacjaThe ends of chromosomes in mammals are composed of telomeric DNA containing TTAGGG repeats, which bind specific proteins called shelterins. This telomeric DNA together with shelterins form a cap that protects the ends of chromosomes from being recognized as sites of DNA damage and from chromosomal fusions. Many very successful antitumor drugs used in the treatment of cancer patients bind to DNA, some of them with a prominent sequence...
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Recent Advances in Loop Heat Pipes with Flat Evaporator
PublikacjaThe focus of this review is to present the current advances in Loop Heat Pipes (LHP) with flat evaporators, which address the current challenges to the wide implementation of the technology. A recent advance in LHP is the design of flat-shaped evaporators, which is better suited to the geometry of discretely mounted electronics components (microprocessors) and therefore negate the need for an additional transfer surface (saddle)...
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Acridinone antitumor agents, C-1305 and C-1299 are metabolized rather by flavin-containing monooxygenases and UDP-glucuronosyltransferases than cytochromes P450
PublikacjaStwierdzono, że to monooksygenazy flawinowe, a nie enzymy cytochromu P450, są głównymi enzymami frakcji mikrosomalnej, odpowiedzialnymi za detoksyfikacyjny metabolizm dwóch badanych pochodnych triazoloakrydonu. Dodatkowo C-1305 ulega sprzęganiu w czasie drugiej fazy metabolizmu z kwasem glukuronowym do postaci O-glukuronidu
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(MgO)n−(n=1–5)Clusters: Multipole-Bound Anions and Photodetachment Spectroscopy
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Synthesis, structure and magnetic properties of neutral Ni(II) tri-tert-butoxysilanethiolate cluster
PublikacjaNowy klaster niklu(II) [Ni6(μ-O)6{SSi(tBuO)3}6(H2O)6(NH3)4] został otrzymany w reakcji NiCl2·6H2O z tri-tert-butoksysilanotiolu i amoniakiem w roztworze wodnym. Pomiary właściwości magnetycznych badanego związku pokazują obecność silnych antyferromagnetycznych oddziaływań pomiędzy centrami metalicznymi.