Search results for: identity
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Characterization of a single-stranded DNA-binding-like Protein from Nanoarchaeum equitans - a nucleic acid binding protein with broad substrate specificity
PublicationBackground SSB (single-stranded DNA-binding) proteins play an essential role in all living cells and viruses, as they are involved in processes connected with ssDNA metabolism. There has recently been an increasing interest in SSBs, since they can be applied in molecular biology techniques and analytical methods. Nanoarchaeum equitans, the only known representative of Archaea phylum Nanoarchaeota, is a hyperthermophilic, nanosized,...
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Characterization of two aminotransferases from Candida albicans
PublicationAminoadipate aminotransferase (AmAA) is an enzyme of α-aminoadipate pathway (AAP) for l-lysine biosynthesis. AmAA may also participated in biosynthesis or degradation of aromatic amino acids and in d-tryptophan based pigment production. The AAP is unique for fungal microorganisms. Enzymes involved in this pathway have specific structures and properties. These features can be used as potential molecular markers. Enzymes catalyzing...
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Employees’ self-expansion as a mediator between perceived work conditions and work engagement and productive behaviors
PublicationThere has been increasing scientific interest in the relationships between self-perception and group identity development processes as well as the behavioural implications of these processes in organizational contexts. Recently, the concept of workplace self-expansion has been introduced to work and organizational psychology. That is, the self-expanding characteristics of work and the workplace have been related to job satisfaction...
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Residual MobileNets
PublicationAs modern convolutional neural networks become increasingly deeper, they also become slower and require high computational resources beyond the capabilities of many mobile and embedded platforms. To address this challenge, much of the recent research has focused on reducing the model size and computational complexity. In this paper, we propose a novel residual depth-separable convolution block, which is an improvement of the basic...
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Generalized access control in hierarchical computer network
PublicationThe paper presents the design of the security layer for a distributed system located in the multizone hierarchical computer network. Depending on the zone from which a client’s request comes to the system and the type of the request, it will be either authorized or rejected. There is one common layer for the access to all the business services and interactions between them. Unlike the commonly used RBAC model, this system enforces...
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Building culture written into the landscape – how to read spatial tradition
PublicationThe article presents a discussion on the experimenting with material leading to practical so-lutions in building structure in the context of the tradition of the place. It sketches out the practical aspects of creation of space while working with different local materials during the intensive workshops within the Structures in Building Culture IP programme. This kind of unique possibility to practice raw materials gives also the...
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Kształtowanie tożsamości na przykładzie małych miast województwa pomorskiego
PublicationSmall towns form an important element of the landscape, economy, both social and cultural life of our regions. Moreover, the way they develop determine regional indicators of the quality of life. These centers take today attempt to redefine its role and place in the spatial structures of the region, looking for new impulses and development opportunities to improve the living conditions of the local public. They have deeply divergent...
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Parseval Wavelet Frames on Riemannian Manifold
PublicationWe construct Parseval wavelet frames in L 2 (M) for a general Riemannian manifold M and we show the existence of wavelet unconditional frames in L p (M) for 1 < p < ∞. This is made possible thanks to smooth orthogonal projection decomposition of the identity operator on L 2 (M), which was recently proven by Bownik et al. (Potential Anal 54:41–94, 2021). We also show a characterization of Triebel–Lizorkin F sp,q (M) and Besov B...
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Evolutionary Algorithm for Selecting Dynamic Signatures Partitioning Approach
PublicationIn the verification of identity, the aim is to increase effectiveness and reduce involvement of verified users. A good compromise between these issues is ensured by dynamic signature verification. The dynamic signature is represented by signals describing the position of the stylus in time. They can be used to determine the velocity or acceleration signal. Values of these signals can be analyzed, interpreted, selected, and compared....
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The dynamic signature verification using population-based vertical partitioning
PublicationThe dynamic signature is an attribute used in behavioral biometrics for verifying the identity of an individual. This attribute, apart from the shape of the signature, also contains information about the dynamics of the signing process described by the signals which tend to change over time. It is possible to process those signals in order to obtain descriptors of the signature characteristic of an individual user. One of the methods...
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A sense of security and freedom in a residential environment
PublicationThe article is based on the results of a survey carried out among students of architecture faculties in several countries, which examined the students’ knowledge of shaping the housing environment in such a way as to enable them to fulfil two basic and, at the same time, seemingly mutually exclusive psychological needs of a person: a sense of security and a sense of freedom. In examining these issues, particular emphasis was placed...
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Urban Design Project III 2023/24
e-Learning Courses# 15-minute CITY ... Gdynia MaŁy Kack The topic of the course will introduce the issues of building a neighborhood identity in the form of densification development. The interdisciplinary team of instructors will introduce the issues of shaping urban structures based on contemporary trends in urban design.
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The Revitalization Processes of the Port Structures in Gdynia and Gdansk on the Background of Contemporary Port Changes
PublicationTransformations of the port facilities against the modernization of the port structures are present in many city-port centers since more than 50 years. The modernization taking place in the ports located in Gdynia-Gdansk mainly concerns communication availability and adapted to the multimodal technology of transport and transshipment. Developing specialized tech-terminals serving a specific type of load, causes development of the...
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CONTRASTING MODERNISMS - ARCHITECTURE OF HARBOUR CITIES GDYNIA AND ALTONA
PublicationThe presentation of the exhibition “Architect Gustav Oelsner – Light, Air, Colour,”, which took place in Gdynia 1.04-29.05.2011, showed the clinker architecture of Gustav Oelsner in Altona. As a contrast to the white-plastered architecture of Gdynia, it provided an interesting background for the comparison of two different faces of modernism. The most important feature of the aesthetics of modernism was its cosmopolitan character,...
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CHROMATOGRAPHIC TECHNIQUES IN PHARMACEUTICAL ANALYSIS IN POLAND: HISTORY AND THE PRESENCE ON THE BASIS OF PAPERS PUBLISHED IN SELECTED POLISH PHARMACEUTICAL JOURNALS IN XX CENTURY
PublicationFor a long time, chromatographic techniques and techniques related to them have stimulated the development of new procedures in the field of pharmaceutical analysis. The newly developed methods, characterized by improved metrological parameters, allow for more accurate testing of, among others, the composition of raw materials, intermediates and final products. The chromatographic techniques also enable studies on waste generated...
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Challenges of branding and marketing of clusters
PublicationClusters have proved to be powerful engine s of economic development in the European Union. Clusters and their stakehol ders can derive many benefits fr om a strong cluster brand. However, they face numerous branding and marketing challenges. Specifics of clusters, such as multiple stakeholders, make it difficult to forge a strong brand identity. This paper addresses the need to develop a cluster specific approach to branding and...
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Architectural Symbols of a City – Case Study
PublicationThe identity of a city is understood as a collection of individual features, which give the city its individual character and distinguish it from other places; it undoubtedly constitutes a cultural value, which should be cherished. In the case of Sopot – a spa located on the Bay of Gdansk, the mosaic of its geographical location, landscape values, urban layout and historic architecture has created a unique image of a seaside resort....
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Minute and diverse in fossil sticky stuff: Tanytarsini (Diptera: Chironomidae) from early Eocene Indian Cambay amber
PublicationWe here present a pioneering systematic review of fossil dipterans of the tribe Tanytarsini (family Chironomidae) discovered in Indian amber from Cambay. The specimens examined belong to five species: Gujaratomyia miripes, Stempellina stebneri sp. nov., Stempellinella pollex sp. nov., Tanytarsus forfex sp. nov. and Tanytarsus ramus sp. nov., which are described. All species belong to the oldest known Tanytarsini and come from the...
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Paradygmat jakościowy w analizie interakcji międzykulturowych – interpretacja na bazie wybranych teorii psychologicznych
PublicationIntercultural interactions in a multicultural work environment are a peculiar type of social interactions. The results of prior research on the effects of interactions in such environment are inconclusive. The majority of the previous studies have emphasized problems, applied a quantitative methodology and interpreted the results with regard to social identity and categorization theory, information-processing theory and intergroup contact...
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A novel methodology for the enantiomeric resolution of methamphetamine, its precursors and intermediates by GC-MS
PublicationImpurity profiling and classification of abused drugs using chiral analytical techniques is of particular interest and importance because of the additional information obtained from this approach. When these methods are applied to the synthesis of illicitly used substances, they can supply valuable information about the conditions/chemicals used in the synthesis. In this study, simultaneous chiral separation of methylamphetamine,...
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Drewno w adoptowanym zabytku - studium przypadku. Budynek nr 2 w dawnych koszarach garnizonu we Wrzeszczu
PublicationThe current structure along with adapted listed buildings and surroundings will create "Garrison of Culture".This part of Gdańsk and Wrzeszcz has been reconnected after eight decades of isolation from the city by joining the square area formed by Grunwaldzka, Słowackiego, Chrzanowskiego, Szymanowskiego streets and the city center. It is a project whitch fits into an aspect of revitalisation related to the socio-economic and environmental...
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In silico epitope prediction of Borrelia burgdorferi sensu lato antigens for the detection of specific antibodies
PublicationDespite many years of research, serodiagnosis of Lyme disease still faces many obstacles. Difficulties arise mainly due to the low degree of amino acid sequence conservation of the most immunogenic antigens among B. burgdorferi s.l. genospecies, as well as differences in protein production depending on the environment in which the spirochete is located. Mapping B-cell epitopes located on antigens allows for a better understanding...
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Isolation of Citrus lemon extracellular vesicles: Development and process control using capillary electrophoresis
PublicationA new and scalable method for the isolation of extracellular vesicles (EV) from Citrus lemon juice samples was developed. The methodology included preliminary preconcentration of the sample using ultrafiltration (UF) followed by size-exclusion chromatography (SEC) purification and final preconcentration of the eluates. Transmission electron microscopy and proteomic analysis showed that isolates contained exosome-like vesicles, exocyst-positive...
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Connected early-career experiences of equality in academia during the pandemic and beyond: Our liminal journey
PublicationIn this paper, we draw on our subjective experiences as two female early-career academics during the global COVID-19 pandemic. While we acknowledge that the pandemic had negative implications for many female scholars due to compulsory telework or increased family responsibilities, we also want to shed light on the empowering experiences shaped by collegial support that became an important part of our pandemic story. We build on...
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Mechanizmy bezpieczeństwa w strefie C systemu netBaltic
PublicationW artykule zaprezentowano rozwiązania zaimplementowane do zabezpieczania komunikacji w warunkach sporadycznej i nieciągłej łączności (Delay Tolerant Networking – DTN) w systemie netBaltic - charakterystycznej dla strefy C tego systemu. Ze względu na dużą różnorodność rozważanych mechanizmów komunikacyjnych, architektura bezpieczeństwa całego systemu została podzielona na kilka elementów – infrastrukturę klucza publicznego (PKI),...
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Thematic Areas in the Polish Countryside and Their Role in Local Development
PublicationThe article defines the phenomenon of space thematisation with regards to rural areas in Poland. The typology of thematic spaces and criteria of their comparative description have been proposed. Thematic villages, home gardens, amusement parks and housing estates have been distinguished. They have been compared in terms of spatial availability, the degree of user participation and the commercial vs. non-commercial creation purpose....
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Tożsamość przedsiębiorcza a stereotypy płci w firmach rodzinnych
PublicationThis article analyses various aspects of women’s experiences in overcoming gender stereotypes that they encounter in family businesses. Historically, entrepreneurship was regarded as a male domain in the literature and entrepreneurs have traditionally been described with typical and stereotypical masculine features. When woman engages in economic activities, she must face the potential incoherence...
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Uprawnienie do uzyskania od administratora informacji o odbiorcach lub o kategoriach odbiorców a cele prawa dostępu do danych. Glosa do wyroku TS z dnia 12 stycznia 2023 r., C-154/21 (RW przeciwko Österreichische Post AG)
PublicationGiven the complexity of modern data processing, individuals often do not know to whom controllers provide the information collected about them. In the commented judgment, the Court ruled that the controller is bound by the data subjects’ request to access information about the identity of the specific recipients of their data and has limited ability to provide them with information about the categories of recipients. This interpretation...
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cDNA fingerprint from the hepatopancreatic glands of pond snails (Lymnaea stagnalis) exposed to benzo[a]pyrene
PublicationIdentification of differentially expressed genes that could be potentially used as biomarkers of PAH exposure of common invertebrate animal (like freshwater snail) would be a valuable resource for investigators interested in toxicology and biomonitoring of aquatic environments. Therefore, the aim of this research was to investigate effects of waterborne benzo[a]pyrene (B[a]P) exposure on mRNA expression in the pond snail’s (Lymnaea...
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Validating data acquired with experimental multimodal biometric system installed in bank branches
PublicationAn experimental system was engineered and implemented in 100 copies inside a real banking environment comprising: dynamic handwritten signature verification, face recognition, bank client voice recognition and hand vein distribution verification. The main purpose of the presented research was to analyze questionnaire responses reflecting user opinions on: comfort, ergonomics, intuitiveness and other aspects of the biometric enrollment...
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Just look at to open it up: A biometric verification facility for password autofill to protect electronic documents
PublicationElectronic documents constitute specific units of information, and protecting them against unauthorized access is a challenging task. This is because a password protected document may be stolen from its host computer or intercepted while on transfer and exposed to unlimited offline attacks. The key issue is, therefore, making document passwords hard to crack. We propose to augment a common text password authentication interface...
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Automatyczna weryfikacja klienta bankowego w oparciu o multimodalne technologie biometryczne
PublicationW referacie przedstawiono przegląd rozwiązań wykorzystywanych w bankach do weryfikacji tożsamości klientów. Ponadto zawarto opis metod biometrycznych aktualnie wykorzystywanych w placówkach bankowych wraz z odniesieniem do skuteczności i wygody korzystania z dostępnych rozwiązań. Zaproponowano rozszerzenie zakresu wykorzystania technologii biometrycznych, wskazując kierunek rozwoju systemów bezpieczeństwa dla poprawy dostępu do...
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Miasto jako marka – analiza wybranych działań marketingowych miasta Lublin
PublicationW niniejszej pracy przedstawiono mechanizmy i proces kształtowania się miasta jako marki. Celem przeprowadzonych badań była analiza wybranych działań marketingowych Lublina od 2007 r. do 2014 r. Zbadano strategię tworzenia się marki „Lublin Miasto Inspiracji” oraz przedstawiono wybrane kampanie promocyjne. Stwierdzono, że dzięki narzędziu Brand Foundations możliwe było skuteczne stworzenie i wprowadzenie marki miasta na rynek....
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Architektoniczne przestrzenie wspólne dizajnu i sztuki
PublicationW aspekcie współczesnego wszechobecnego konsumpcjonizmu i ograniczonych możliwości dotarcia do odbiorcy, role obiektów należących do sztuk czystych przejmują obiekty będące wytworami projektantów w zakresie sztuk użytkowych, w tym architektów. Jednocześnie sztuka poszukuje nowych form i środków wyrazu, żeby wzbudzić zainteresowanie. Rozpatrywanym obiektem jest Dom. Oczywistą jego odsłoną jest spełnienie oczekiwanej funkcji wynikającej...
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Company Culture, Knowledge Sharing and Organizational Performance. The Employee’s Perspective
PublicationKnowledge sharing, as a basic prerequisite for knowledge creation, is a dynamic social process characterized by profound human interactions. The process of knowledge sharing can be supported by organizational culture which is a set of values and norms giving identity to each enterprise. As a valuable element of intellectual capital, organizational culture contributes to achieving strategic business goals. The purpose of this article...
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Travel agents and destination management organizations: eLearning as a strategy to train tourism trade partners.
PublicationThis article offers an overview of the existing online courses run by national destination management organizations (DMOs) in order to better equip travel agents and tour operators in the sales activities of the tourism destinations. These online courses represent one of the B2B offers by DMOs and an interesting opportunity for travel agents, who are trying to find their identity and competitive advantage within the context of...
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An MTA-phosphorylase gene discovered in the metagenomic library derived from Antarctic top soil during screening for lipolytic active clones confers strong pink fluorescence in the presence of rhodamine B.
PublicationIn this work, we present the construction of a metagenomic library in Escherichiacoli using the pUC19 vector and environmental DNA directly isolated fromAntarctic topsoil and screened for lipolytic enzymes. Unexpectedly, the screeningon agar supplemented with olive oil and rhodamine B revealed one unusual pinkfluorescent clone (PINKuv) out of 85 000 clones. This clone harbored a plasmid,pPINKuv, which has an insert of 8317 bp that...
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Identification and molecular modeling of a novel lipase from an Antarctic soil metagenomic library.
PublicationIn this work, we present the construction of a metagenomic library in Escherichia coli using pUC19 vector and environmental DNA directly isolated from Antarctic topsoil and screened for lipolytic enzymes. The screening on agar supplemented with olive oil and rhodamine B revealed one clone with lipolytic activity (Lip1) out of 11,000 E. coli clones. This clone harbored a plasmid, pLip1, which has an insert of 4722 bp that has been...
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Politics and urban artifacts in cities: what are they and how are they interconected?
PublicationThis research, analyses the effect of politics on a city and its image, via their control over the formation or changes on urban artifacts. It analyses the concepts introduced in Aldo Rossi’s architectural monograph, The Architecture of the City, in the chapter titled ‘Politics as Choice’, and his idea that a city’s image is a reflection of its political institutions and the choices people in authority make. Monuments and architecture...
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Identification and properties of the psychrophilic bacterium Desulfolatea psychrophila single-stranded DNA binding proteins
PublicationTo study the biochemical properties of SSB from Desulfolatea psychrophila (DpsSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 426 nucleotides encoding SSB protein of 142 amino acids with a calculated molecular mass of 15.6 kDa and it is the smallest known bacterial SSB protein indentified to date. The amino-acid sequence...
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Two highly thermostable paralogous single-stranded DNA-binding proteins from Thermoanaerobacter tengcongensis
PublicationThe thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. coli overexpression systems. The TteSSB2 and TteSSB3 consist of 153 and 150 amino acids with a calculated molecular...
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Extended micropolar approach within the framework of 3M theories and variations thereof
PublicationAs part of his groundbreaking work on generalized continuum mechanics, Eringen proposed what he called 3M theories, namely the concept of micromorphic, microstretch, and micropolar materials modeling. The micromorphic approach provides the most general framework for a continuum with translational and (internal) rotational degrees of freedom (DOF), whilst the rotational DOFs of micromorphic and micropolar continua are subjected...
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Identification of Substrates of Cytoplasmic Peptidyl-Prolyl Cis/Trans Isomerases and Their Collective Essentiality in Escherichia Coli
PublicationProtein folding often requires molecular chaperones and folding catalysts, such as peptidyl-prolyl cis/trans isomerases (PPIs). The Escherichia coli cytoplasm contains six well-known PPIs, although a requirement of their PPIase activity, the identity of their substrates and relative enzymatic contribution is unknown. Thus, strains lacking all periplasmic and one of the cytoplasmic PPIs were constructed. Measurement of their PPIase...
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A highly thermostable, homodimeric single-stranded DNA-binding protein from Deinococcus radiopugnans
PublicationWe report the identification and characterization of the single-stranded DNA-binding protein (SSB) from the mesophile and highly radiation-resistant Deinococcus radiopugnans (DrpSSB). PCR-derived DNA fragment containing the complete structural gene for DrpSSB protein was cloned and expressed in Escherichia coli. The gene consisting of an open reading frame of 900 nucleotides encodes a protein of 300 amino acids with a calculated...
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Perceived neighborhood disorder and quality of life: The role of the human-place bond, social interactions, and out-group blaming.
PublicationFour studies were carried out to examine how neighborhood disorder, human-place bonds, relationships among neighbors, and urban quality of life are related and what role blaming nonresidents plays in these relationships. In the first study, local identity was a significant mediator of the relationship between neighborhood disorder and quality of life. In the second study, we found that participants exposed to vignettes describing...
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Identyfikacja i izolacja genu kodującego zaadaptowaną do zimna β-galaktozydazę Paracoccus sp. = Identification and izolation of the gene encoding cold-active β-galactosidase from paracoccus sp.
PublicationZaprezentowane badania były prowadzone u eehi wyizolowania ze środo¬wiska organizmu psychrofilnego, zdolnego do hydrolizy laktozy w niskiej tempe¬raturze, oraz zidentyfikowania genu kodującego (i-galaktozydazę zaadaptowaną do zimna.Poszukiwan gen został w izolowany z psychrofilnego szczepu autarkicz¬nego Paracoccus sp. 321). Na podstawie sekwencji nukleotydowej genu kodują¬cego fS-galaktozdaze ustalono skład aminokwasów),...
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MEDIA ARCHITECTURE AND INTERACTIVE ART INSTALLATIONS STIMULATING HUMAN INVOLVEMENT AND ACTIVITIES IN PUBLIC SPACES
PublicationThis paper examines the potential for media architecture and interactive art installations to stimulate human involvement and activities in public spaces. On the basis of theoretical approaches, case studies and interdisciplinary surveys, the paper provides insight into how screens projecting media and interactive installations in city spaces can inspire people to become active on many levels. The research is focused, both on temporary...
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Novel single-stranded DNA-binding protein from psyvhrophilic bacterium Psychrobacter arcticus
PublicationTo study the biochemical properties of SSB from Psychrobacter arcticus (ParSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 642 nucleotides encoding SSB protein of 213 amino acids with a calculated molecular mass of 22.8 kDa. The amino-acid sequence of ParSSB exhibits 49% identity and 57% similarity to Escherichia coli SSB....
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Experiencing historic waterways and water landscapes of the Vistula River Delta
PublicationThe paper gives insights into the characteristics of Vistula River Delta water landscapes, referring to the process of creating land out of water by Polish, Dutch and German settlers. Based on this, the paper presents an integrated strategy formed upon studies in cultural geography and aesthetics, and focused on enhancing the unique characteristics of the region and its rediscovery by tourism. The long process of constructing Vistula...
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INDUSTRIAL HERITAGE IN “SACRIFICE ZONES”. THE POTENTIAL OF BOCAMINA I & II THERMOELECTRIC IN CORONEL, CHILE
PublicationThis work aims to present the recovery potential of the Chilean Sacrifice Zones, urban areas affected by high amounts of pollution caused by industrial activities. It centers in the case of “Bocamina I & II”, two Thermoelectric based in the city of Coronel, southern Chile. A settlement historically related to the mining processes. These plants operated for decades supplying the national energy grid and are seen as both symbols...