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An examination of an Antarctic soil metagenomic-derivate putative methylthioadenosine phosphorylase gene as a novel reporter gene for promoter trapping
PublicationThe rsfp gene was discovered during examination ofan Antarctic soil-derived metagenome library and encodesa bacterial methylthioadenosine phosphorylase (GenBank accession number GQ202582). In a previous study, we found that E. coli colonies expressing the rsfp gene reveal strong pink fluorescence after exposure to UV light when grown on culture medium supplemented with rhodamine B (Cieśliński et al., 2009). In contrast to that,...
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New Architecture of Solid-State High-Voltage Pulse Generators
PublicationThe application of the nanosecond pulsed electric field (nsPEF) for biomedical treatments has gained more interest in recent decades due to the development of pulsed power technologies which provides the ability to control the electric field dose applied during tests. In this context, the proposed paper describes a new architecture of solid-state high-voltage pulse generators (SSHVPG) designed to generate fully customised sequences...
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Pedestrian detection in low-resolution thermal images
PublicationOver one million people die in car accidents worldwide each year. A solution that will be able to reduce situations in which pedestrian safety is at risk has been sought for a long time. One of the techniques for detecting pedestrians on the road is the use of artificial intelligence in connection with thermal imaging. The purpose of this work was to design a system to assist the safety of people and car intelligence with the use...
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Super-resolved Thermal Imagery for High-accuracy Facial Areas Detection and Analysis
PublicationIn this study, we evaluate various Convolutional Neural Networks based Super-Resolution (SR) models to improve facial areas detection in thermal images. In particular, we analyze the influence of selected spatiotemporal properties of thermal image sequences on detection accuracy. For this purpose, a thermal face database was acquired for 40 volunteers. Contrary to most of existing thermal databases of faces, we publish our dataset...
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The possibility of estimating the height of the ionospheric inhomogeneities based on TEC variations maps obtained from dense GPS network
PublicationA state of the ionosphere can be effectively studied using electromagnetic signals received from global navigation satellite systems (GNSS). Utilization of the dual frequency observations allows estimating values of the total electron content (TEC). They can be used for a number of scientific studies such as detection and monitoring of traveling ionospheric disturbances or plasma bubbles. Moreover, maps of TEC variations allow...
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Effect of Individual Components of Lignocellulosic Biomass on Methane Production and Methanogen Community Structure
PublicationOne of the major factors that influences the economic feasibility of biogas production is the availability of digestible feedstocks. There is little research on the influence of the chemical composition of biomass on biogas synthesis, especially with regard to the content of lignocellulosic materials. Therefore, the aim of this study was to estimate how differences in the content of cellulose and lignin in lignocellulosic biomass...
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Gesture Recognition With the Linear Optical Sensor and Recurrent Neural Networks
PublicationIn this paper, the optical linear sensor, a representative of low-resolution sensors, was investigated in the multiclass recognition of near-field hand gestures. The recurrent neural network (RNN) with a gated recurrent unit (GRU) memory cell was utilized as a gestures classifier. A set of 27 gestures was collected from a group of volunteers. The 27 000 sequences obtained were divided into training, validation, and test subsets....
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DST-Based Detection of Non-cooperative Forwarding Behavior of MANET and WSN Nodes
Publication. Selfish node behavior can diminish the reliability of a mobile ad hoc network (MANET) or a wireless sensor network (WSN). Efficient detection of such behavior is therefore essential. One approach is to construct a reputation scheme, which has network nodes determine and share reputation values associated with each node; these values can next be used as input to a routing algorithm to avoid end-to-end routes containing ill-reputed...
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Enzyme immobilization on magnetic nanoparticles
PublicationImmobilized enzymes have been commonly used in many branches of industry, biotechnology, medicine and also in analytical chemistry. Their use provides many advantages in comparison with free enzymes including repeated, easy recovery of the enzyme and improvement in enzyme stability. Enzymes are strong and specific catalysts, but are also expensive and fragile. Their immobilization and stabilization may reduce reaction costs, which...
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Framework for RIDM within functional safety management process
PublicationThe functional safety management in life cycle is a complex process starting with identifying hazards and defining safety-related functions (SRFs) with risk assessment oriented at determining the safety integrity level. Another element of such life cycle is a verification of required SIL for designed safety-related system which implements safety function. Due to complexity of the problem, to overcome difficulties in safety-related...
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Odnowa modelu biznesu
PublicationW artykule przedstawiono wyniki badań teoretycznych nad istotą odnowy strategicznej biznesu. Odnowę tę oparto na restauracji ciągu zarządzania strategicznego.
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Conditions controlling atmospheric pollutant deposition via snowpack
PublicationSolid precipitation represents a potentially important addition to other measures of deposition. However, an accurate estimate of snowfall amount and pollutant loading is not a trivial matter. There are obvious distinctions between regular precipitation collection and snowpack sampling that represent the cumulative chemistry of bulk deposition. The main goal is to show the most important processes and factors that may influence...
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Entire fracture surface topography parameters for fatigue life assessment of 10H2M steel
PublicationIn this paper, the entire fracture surface approach was used to assess an effect of 280,000 h of exploitation under internal pressure of 2.9 MPa and high temperature of 540 °C on the fatigue response of 10H2M (10CrMo9–10) power engineering steel. The mechanical testing was carried out on the hourglass specimens produced from the as-received, unused pipeline and the same material after long-time exploitation. The uniaxial tensile...
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Addressing Challenges in AI-based Systems Development: A Proposal of Adapted Requirements Engineering Process
Publication[Context] Present-day IT systems are more and more dependent on artificial intelligence (AI) solutions. Developing AI-based systems means facing new challenges, not known for more conventional systems. Such challenges need to be identified and addressed by properly adapting the existing development and management processes. [Objective] In this paper, we focus on the requirements engineering (RE) area of IT projects and aim to propose...
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The Structure of Entrepreneurial Team Members’ Competencies: Between Effectuation and Causation
PublicationA conscious shaping of entrepreneurial competence is a relevant element of entrepreneurial education. In order to recognize which of the competencies regarded as entrepreneurial are characteristic of members of entrepreneurial teams, which is crucial for the work of those teams, it is necessary to identify the structure of those competencies. This quantitative study was conducted with the use of a survey method and involved 111...
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Size effect at aggregate level in microCT scans and DEM simulation – Splitting tensile test of concrete
PublicationThe paper describes an experimental and numerical study of size effect on concrete cylindrical specimens in splitting tensile test. Own experimental campaign was performed on specimens with 5 various diameters from D = 74, 105, 150, 192 and 250 mm with hardboard loading strips (distributed load according to standard methods) scaled proportionally to the specimen diameter. The crack opening-control system was applied to obtain the...
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Typology of low developed non-metropolitan sub-regions in the European Union
PublicationThis paper shows a typology of 319 NUTS-3 sub-regions in the European Union. The selected sub-regions are non-metropolitan sub-regions, whose gross domestic product per capita in 2011 was below 75% of the EU average. The objective of this typology was to recognize diversity in the examined sub-regions as a starting point for the selection of areas for future comparative research. The typology takes into account 24 variables related...
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Highly Active TiO2 Microspheres Formation in the Presence of Ethylammonium Nitrate Ionic Liquid
PublicationSpherical microparticles of TiO2 were synthesized by the ionic liquid-assisted solvothermal method at different reaction times (3, 6, 12, and 24 h). The properties of the prepared photocatalysts were investigated by means of UV-VIS diffuse-reflectance spectroscopy (DRS), Brunauer–Emmett–Teller (BET) surface area measurements, scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), and X-ray photoelectron spectroscopy...
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Influence of the air phase on water flow in dikes
PublicationNumerical models are often used to describe flow and deformation processes occurring in dikes during flood events. Modeling of such phenomena is a challenging task, due to the complexity of the system, consisting of three material phases: soil skeleton, pore water and pore air. Additional difficulties are transient loading caused by variable in time water levels, heterogeneity of the soil or air...
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Mono- and bi-metallic plasmonic photocatalysts for degradation of organic compounds under UV and visible light irradiation
PublicationTitania powders were surface modified with gold and/or silver nanoparticles (NPs) by photodeposition method. Gold modified titania exhibited much higher photocatalytic activity during methanol dehydrogenation under UV irradiation than titania modified with monometallic silver and bimetallic Au–Ag NPs. Bimetallic photocatalysts exhibited either enhanced or reduced visible light activity, depending on properties of noble metal NPs,...
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Computer-Aided Design of Railroad Horizontal Arc Areas in Adapting to Satellite Measurements
PublicationThis paper presents a method of designing railway sections located in horizontal arcs. The adopted procedure is universal, i.e., it creates the possibility of varying both the type and the length of the assumed transition curves. This means that the applied analytical formulas apply to the boundary conditions of the transition curves and all of the simplifications widely existing in common algorithms have been eliminated. The presented...
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A Method for Underwater Wireless Data Transmission in a Hydroacoustic Channel under NLOS Conditions
PublicationWireless data transmission in the hydroacoustic channel under non-line-of-sight (NLOS) propagation conditions, for example, during a wreck penetration, is difficult to implement reliably. This is mostly due to the multipath propagation, which causes a reduction in the quality of data reception. Therefore, in this work an attempt has been made to develop a reliable method of wireless underwater communication test it under the NLOS...
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Methodology of generation of CFD meshes and 4D shape reconstruction of coronary arteries from patient-specific dynamic CT
PublicationDue to the difficulties in retrieving both the time‑dependent shapes of the vessels and the generation of numerical meshes for such cases, most of the simulations of blood flow in the cardiac arteries use static geometry. The article describes a methodology for generating a sequence of time‑dependent 3D shapes based on images of different resolutions and qualities acquired from ECG‑gated coronary artery CT angiography. The precision...
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Automatic labeling of traffic sound recordings using autoencoder-derived features
PublicationAn approach to detection of events occurring in road traffic using autoencoders is presented. Extensions of existing algorithms of acoustic road events detection employing Mel Frequency Cepstral Coefficients combined with classifiers based on k nearest neighbors, Support Vector Machines, and random forests are used. In our research, the acoustic signal gathered from the microphone placed near the road is split into frames and converted...
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α-Pinene, 3-carene and d-limonene in indoor air of Polish apartments: The impact on air quality and human exposure
PublicationMonoterpenes are among most ubiquitous volatile organic compounds (VOCs) to be detected in indoor air. Since the quality of indoor air is considered important for inhabitants' well-being, the present study aimed at investigating impact of human activity on levels of selected monoterpenes applying passive sampling technique followed by thermal desorption and gas chromatography coupled mass spectrometry. One of the objectives of...
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Homoisocitrate dehydrogenase from Candida albicans: properties, inhibition, and targeting by an antifungal pro-drug
PublicationThe LYS12 gene from Candida albicans, coding for homoisocitrate dehydrogenase was cloned and expressed as a His-tagged protein in Escherichia coli. The purified gene product catalyzes the Mg2+- and K+-dependent oxidative decarboxylation of homoisocitrate to alpha-ketoadipate. The recombinant enzyme demonstrates strict specificity for homoisocitrate. SDS-PAGE of CaHIcDH revealed its molecular mass of 42.6±1 kDa, while in size-exclusion...
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Superconductivity and appearance of negative magnetocaloric effect in Ba1–xKxBiO3 perovskites, doped by Y, La and Pr
PublicationRecently, substantial attention is given to the bismuth-based perovskites for variety of electronic applications. The perovskites are used for quantum dots displays (QLED), photovoltaic systems and superconducting (HTS) devices. In this paper comprehensive studies of Ba1–xKxBi1–yREyO3 bismuth perovskites (REBKBO, RE = Y , La or Pr) are reported. Apart from structural anomalous behavior at low temperatures, the electronic properties...
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Design centering of compact microwave components using response features and trust regions
PublicationFabrication tolerances, as well as uncertainties of other kinds, e.g., concerning material parameters or operating conditions, are detrimental to the performance of microwave circuits. Mitigating their impact requires accounting for possible parameter deviations already at the design stage. This involves optimization of appropriately defined statistical figures of merit such as yield. Alt-hough important, robust (or tolerance-aware)...
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Shooting Gas Cylinders to Prevent Their Explosion in Fire
PublicationGas cylinders in general, and particularly cylinders containing acetylene, constitute a great potential danger to fire and rescue services involved in salvage operations. Experiments show that gas cylinders with acetylene, oxygen, hydrogen, CNG, LPG or CO2 may blow after short exposition to heat with very destructive effect as fragments of blown cylinder may fly even several hundred meters. In the case of acetylene the explosion...
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Focus on Misinformation: Improving Medical Experts’ Efficiency of Misinformation Detection
PublicationFighting medical disinformation in the era of the global pandemic is an increasingly important problem. As of today, automatic systems for assessing the credibility of medical information do not offer sufficient precision to be used without human supervision, and the involvement of medical expert annotators is required. Thus, our work aims to optimize the utilization of medical experts’ time. We use the dataset of sentences taken...
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Impact performance of novel multi-layered prepacked aggregate fibrous composites under compression and bending
PublicationMulti-layered Prepacked aggregate fibrous composite (MLPAFC) is a new type of concrete, which is prepared in two subsequent stages of aggregate-fibre skeleton prepacking and cementitious grouting. In this study, ten MLPAFC mixtures were prepared in three subsequent layers incorporating different contents of four different types of steel fibres. Long and short hooked-end and crimped steel fibers were adopted with 3.0 and 1.5% dosages...
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ALIS-FLP: amplified ligation selected fragment-length polymorphism method for microbial genotyping
PublicationA DNA fingerprinting method known as ALIS-FLP (amplified ligation selected fragment-length polymorphism) has been developed for selective and specific amplification of restriction fragments from TspRI restriction endonuclease digested genomic DNA. The method is similar to AFLP, but differs in that only one specific restriction enzyme (TspRI) is used. The cohesive ends of the DNA fragments are ligated with two types of oligonucleotide....
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Multimedia distributed system for visualization of ongoing and archival events for BG
PublicationThe paper presents concept of the distributed system designed to gather and provide the information about vehicles, vessels and airplanes present within the area of operations of the Border Guard supplemented with related multimedia. The part of the system related to the map data gathering, distribution and visualization has been already implemented in the preceding project. The presented system is the expansion of the previous...
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The organophosphorus sulfenyl bromides as versatile reagents for cysteine derivatives functionalization by unsymmetrical disulfide bond formation
PublicationWe have developed a convenient method for the synthesis of L-cysteine unsymmetrical disulfides under mild conditions with good to excellent yields. Described method is based on the straightforward preparation of the organophosphorus sulfenyl bromide readily available from bis-(5,5-dimethyl-2-thiono-1,3,2-dioxaphosphorinanyl) disulfide. The unsymmetrical disulfides can be obtained for L-cysteine derivatives and thiols bearing neutral...
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Author Reply to “Regarding ‘Repair Augmentation of Unstable, Complete Vertical Meniscal Tears With Bone Marrow Venting Procedure: A Prospective, Randomized, Double-Blind, Parallel-Group, Placebo-Controlled Study’”
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Repair Augmentation of Unstable, Complete Vertical Meniscal Tears With Bone Marrow Venting Procedure: A Prospective, Randomized, Double-Blind, Parallel-Group, Placebo-Controlled Study
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Extraction Methods of 10-Deacetylbaccatin III, Paclitaxel, and Cephalomannine from Taxus baccata L. Twigs: A Comparison
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Application of Sea Sand Disruption Method for HPLC Determination of Quercetin in Plants
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Optimization of ASE Conditions for the HPLC Determination of Rutin and Isoquercitrin inSambucus nigraL
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Retention modeling of some saccharides separated on an amino column.
PublicationUsing an amino column (Supelcosil LC-NH2) and different mixtures of acetonitrile-water, quantitative structure-retention relationship models are discussed. These models are based on computed molecular descriptors representing numerically structured features of some saccharides. The obtained results are underlining the lipophilicity/hydrophilicity balance, and how this is controlling the separation of the saccharides. The resulting...
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N-(diphenoxyphosphoryl)succinimide:synthesis and reaction with alkoxides
PublicationReakcja sukcynoimidu z chlorkiem difenylofosforowym w obecności lda a prowadzi do powstania amidofosforanu. ta sama reakcja prowadzona w obecności wodorku sodu pozwala na otrzymanie fosforylowanego sukcynoimidu, który w reakcji z alkoholanami w nasteptwie otwarcia pierścienia, prowadzi do do analogów amidofosforanu z dobrymi wydajnościami.
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Synthesis and Properties of Di-isopropylamino Derivatives of Diphosphanes and Triphosphanes: The X-Ray Structure of (iPr2N)2P-P(SiMe3)2
PublicationPrzeprowadzono reakcję (iPr2N)2PCl z P(SiMe3)2Li w wyniku której otrzymano difosfan (iPr2N)2P-P(SiMe3)2. Strukturę związku określono przy pomocy rentgenowskiej analizy strukturalnej. Otrzymano także pochodną litową (iPr2N)2P-P(SiMe3)Li w rekcji z nBuLi. W reakcji (iPr2N)2PCl z (iPr2N)2P-P(SiMe3)Li otrzymano trifosfan (iPr2N)2P-P(SiMe3)-P(NiPr2)2 z dużą wydajnością. Próby otrzymania pochodnej litowej (iPr2N)2P-PLi-P(NiPr2)2 zakończyły...
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Gdynia - a New City of the Twenties and Thirties
PublicationArtykuł jest pierwszym przedstawieniem w zagranicznym, anglojęzycznym czasopiśmiennictwie dziejów budowy nowego miasta lat dwudziestych i trzydziestych XX w, jakim był Gdynia. Przedstawiona została geneza decyzji o budowie portu i miasta, pierwsze plany urbanistyczne w kontekście projektów portu, a także rozwój architektoniczny Gdyni. W szczególności zaś przedstawiono przemiany stylowe gdyńskiej architektury modernistycznej tego...
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Potassium Tri-tert-butoxysilanethiolates: Synthesis, Properties, and Crystal and Molecular Structures of [(tBuO)3SiSK]6[THF]2·2THF, {[(tBuO)3SiS]2K2}n, and [(tBuO)3SiSK]4[H2O]4·C6H6
PublicationW reakcji tri-tert-butoksysilanotiolu z potasem otrzymano trzy nowe związki:[(tBuO)3SiSK]6[THF]2·2THF, {[(tBuO)3SiS]2K2}n, i[(tBuO)3SiSK]4[H2O]4·C6H6. Stanowią one dogodne substraty w syntezach kompleksów silanotiolanowych metali przejściowych. Charakteryzują się one również różnorodnością tworzonych układów. Rentgenowska analiza strukturalna wykazała, iż tworzą one trzy typy makrocząsteczkowych układów, w których...
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Synthesis of some optically active terpenoid-derived thioamides
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Calculation of the standard potentials of the dithiocarbamate/thiuram disulfide redox system via thermochemical cycles and computational electrochemistry .
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The reactions of dialkyl phosphites and phosphine oxides with iodosylbenzene
PublicationThe reaction of iodosylbenzene with >P(O)H type of acids (dialkyl phosphites, secondary phosphine oxides) was studied. The acids of >P(O)H type add to iodosylbenzene to yield intermediate 6 which in the aprotic solvents yields oxidation products, it means >P(O)OH acids and/or anhydride of >P(O)OP(O)< type. On the other hand if the reaction is performed in alcohol as a solvent in the presence of sodium alcoholate >P(O)OR ester is...
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May dithiophosphoric acid participate in the set process?
PublicationZastosowano otrzymane S-tioacyloditiofosforany jako odczynniki tioacylujące do syntezy kwasów tiohydroksamowych. Dla większości N-alkilohydroksyloamin produktem tej reakcji były żądane kwasy tiohydroksamowe. Natomiast gdy substratem jest hydroksyloamina zawierająca duży objętościowo podstawnik na atomie azotu lub gdy posiada dwa podstawniki na atomie azotu w reakcji tej tworzą się wyłącznie odpowiednie O-tioacylohydroksyloaminy....
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Excited states of isoxazole molecules studied by electron energy-loss spectroscopy
PublicationElectron energy-loss spectra were measured in isoxazole in the excitation energy range 3.5−10 eV to investigate the valence excited states. Spectra recorded at different scattering conditions enabled the identification of the singlet and triplet states and the determination of their vertical excitation energies. The two lowest energy triplet bands, ππ* 13A' and ππ* 23A' at 4.20 and 5.30 eV, respectively show vibrational progressions....
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A Novel [4+2] Cycloaddition Reaction Involving Lawesson’s Reagent. Structure and Specific Fragmentations of a New Cyclic 1,2-Thiaphosphinane-4-one
PublicationA crude morpholine enamine of acetone treated with Lawesson’s reagent unexpectedly yielded a six-membered thiaphosphinane-4-one. This compound is the first example of a new class of heterocycles. It has been proven that it is formed from 4-methyl-2-morpholino-1,3-pentadiene which is usually present in crude morpholine enamine batches. A mechanism of this regioselective reaction was postulated and a characteristic chair-like conformation...