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Search results for: MP-PCR
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Modified polycarbonate urethane: synthesis, properties and biological investigation in vitro
PublicationNowy poliweglano uretan (PCU-I) otrzymano z alifatycznych monomerów: poliwęglanu heksanodiolu, 4,4'diizocyjanianu dicykloheksanometanu, mieszaniny małocząsteczkowych dioli i oleju rycynowego (zawierającego głównie trigliceryd kwasu 12-hydroksyoleinowego). PCU-II otrzymano analogicznie ale bez udziału oleju rycynowego. Obydwa PCU charakteryzowały się dobrą wytrzymałością na rozciąganie. PCU-I miał niższą temperaturę zeszklenia a...
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Prediction of protein assemblies, the next frontier: The CASP14‐CAPRI experiment
PublicationWe present the results for CAPRI Round 50, the 4th joint CASP-CAPRI protein assembly prediction challenge. The Round comprised a total of 12 targets, including 6 dimers, 3 trimers, and 3 higher-order oligomers. Four of these were easy targets, for which good structural templates were available either for the full assembly, or for the main interfaces (of the higher-order oligomers). Eight were difficult targets for which only distantly...
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Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: control of an outbreak using a new ADSRRS technique
PublicationSzczepy Klebsiella pneumoniae (EPKP)produkujące beta-laktamazy o rozszerzonym spektrum substratowym (ESBL)stanowią często problem zakażeń na oddziałach noworodkowych. Izolaty od 149 pacjentów poddano typowaniu nową metodą opartą na zjawisku supresji PCR, ADSRRS-fingerprinting, wykazując pochodzenie klonalne badanych szczepów. Użyteczność techniki ADSRRS-finerprinting została potwierdzona innymi metodami - RAPD i PFGE.
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Novel thermostable single-stranded DNA-binding protein (SSB) from Deinococcus geothermalis
PublicationW celu zbadania właściwości biochemicznych białka wiążącego się do jednoniciowego DNA (SSB) pochodzącego z Deinococcus geothermalis (DgeSSB), sklonowano gen ssb otrzymany w reakcji PCR, a następnie uzyskano wydajną biosyntezę białka DgeSSB. Gen składa się z 900 par zasad, kodujących białko złożone z 300 reszt aminokwasowych, o wyliczonej masie molekularnej równej 32.45 kDa. Sekwencja aminokwasowa wykazuje 43, 44% i 75% identyczności...
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Degradation of Polycaptolactone Modified with TPS or CaCO3 in Biotic/Abiotic Sewater
PublicationThe polymer degradation process in two types of water sourced from the Baltic Sea was investigated. Polycaprolactone modified with thermoplastic starch(PCL/TPS>85%) or calcium carbonate (60% PCL/40% CaCO3)and PCL alone were incubated for 28 weeks in sea water with and without microrganisms. The experimental results indicated that in each of the experiments degradation occured. The process was more effective in sea water with microorganisms.Modification...
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Thermostable Pyrococcus woesei beta-D-galactosidase - high level expression, purification and biochemical properties
PublicationGen kodujący termostabilną beta-D-galaktozydazę Pyrococcus woesei zamplifikowano z zastosowaniem techniki PCR i klonowano w komórkach Escherichia coli. Skonstruowany system ekspresji genu pozwolił na uzyskanie znacznej ilości termostabilnego białka w komórkach mezofilnego gospodarza. Enzym oczyszczono z zastosowaniem chromatografii powinowactwa po wcześniejszej denaturacji termicznej białek Escherichia coli i scharakteryzowano...
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Total mercury in great cormorant (Phalacrocorax carbo) and fish in polish sector of Vistula Lagoon
PublicationAnalizowano tkanki miękkie 55 osobników kormorana czarnego oraz wybrane gatunki ryb stanowiące ich pokarm. Badane próbki pochodziły z ekosystemu Zalewu Wiślanego. Zawartość rtęci oznaczono w narządach wewnętrznych kormorana czarnego takich jak: wątroba, nerki, mięśnie, przełyk, żołądek, jelita, tchawica, płuca, mięsień sercowy. Przebadano następujące gatunki ryb: śledź, stynka, karaś, karaś srebrzysty, płoć, lin, jazgarz. Oznaczenia...
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Development of the New Polish Method for Capacity Analysis of Motorways and Expressways
PublicationThe paper presents development of the new Polish method for performing capacity analysis of basic segments of dual carriageway roads (motorways and expressways). The method is based on field traffic surveys conducted at 30 motorway and expressway sites (class A and S roads) in Poland. Traffic flows, composition and travel times were observed in 15-min intervals at each site using ANPR filming method. These data were used to calibrate...
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Potential of novel atomic emission techniques as a tool for investigation of the possibilities of using industrial waste as additives in construction materials
PublicationNowadays, due to the global ecological crisis and ways to prevent a climate disaster, more and more attention is paid to green techno-logies and green Chemistry, which are part of the so-called “The Green Deal.” The main assumption is the optimization of the processes of producing necessary goods and the implementation of methods of managing postproduction waste in the least environmentally harmful manner. In the presented research,...
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Development of Gas Sensor Array for Methane Reforming Process Monitoring
PublicationThe article presents a new method of monitoring and assessing the course of the dry methane reforming process with the use of a gas sensor array. Nine commercially available TGS chemical gas sensors were used to construct the array (seven metal oxide sensors and two electrochemical ones). Principal Component Regression (PCR) was used as a calibration method. The developed PCR models were used to determine the quantitative parameters...
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Podstawy Sterowania Komputerowego - 2023
e-Learning Courses6 semestr ACR (Automatyka, Cybernetyka i Robotyka)
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Podstawy Sterowania Komputerowego - 2022
e-Learning Courses6 semestr ACR (Automatyka, Cybernetyka i Robotyka)
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PSK_2025
e-Learning Courses6 semestr ACR (Automatyka, Cybernetyka i Robotyka)
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Karol Grębowski dr inż.
PeopleKarol Grębowski (M.Sc.) works as an assistant at the Department of Technical Fundamentals of Architecture Design of the Faculty of Architecture at Gdansk University of Technology. His scientific research deals with dynamic phenomena occurring during the vibration of structures, bridges, and studies the design methodology of the elements forming passive protection system of the vehicles degraded by struck or explosion in the context...
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Tomasz Wąsowicz dr hab.
PeopleTomasz Wąsowicz's research was first related to high-resolution atomic spectroscopy and focused on measurements and analysis of the transition probabilities of the forbidden lines, the hyperfine and isotopic structure of spectral lines of heavy elements, Stark effect in the helium atom. Tomasz Wąsowicz currently studies physicochemical processes occurring during interactions of various forms of radiation with atoms and molecules...
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Experimental investigations on the mechanical properties and damage detection of carbon nanotubes modified crumb rubber concrete
PublicationThis study presents a modified crumb rubber (MCR) concrete design mix reinforced with multi-walled carbon nanotubes (MWCNTs), mechanical characterization, and cracking monitoring using the acoustic emission (AE) technique. The results showed that the bridging effect of MWCNTs and MCR in the concrete mix mitigated the shortcomings of MWCNT-MCR concrete and improved the flexural and compressive strengths by 18.3% and 26.5%, respectively,...
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Konsultacje - Sterowanie Cyfrowe (ACR)
e-Learning CoursesMateriały do Sterowania Cyfrowego
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Technika Mikroprocesorowa 2023 - ACR
e-Learning CoursesTechnika Mikroprocesorowa dla ACR - materiały: wykład i laboratorium.
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Technika Mikroprocesorowa dla ACR
e-Learning CoursesProjektowanie systemów mikroprocesorowych i programowanie w asemblerze.
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Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus - expression and purification
PublicationBiałka SSB są niezbędne do życia każdemu organizmowi. W tej pracy prezentujemy wyniki otrzymania i oczyszczania białek SSB Thermus thermophilus i T. aquaticus. Dotąd były znane białka SSB jedynie o budowie homotetrameru lub heterotrimeru. Białka SSB Thermus thermophilus i T. aquaticus stanowią nową klasę białek SSB - białek homodimerycznych. Ponadto dowiedziono, iż białka te są użyteczne w reakcji PCR.
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Maciej Sperczyński
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A procedure for the determination of dichloromethane and tetrachloroethene in water using pervaporation and gas chromatography
PublicationIn the present study, pervaporation was applied to the determination of tetrachloroethene (PCE) and dichloromethane (DCM) in liquid samples. PCE is the most commonly used solvent in dry-cleaning processes. PCE belongs to group 2A of carcinogens (probably carcinogenic to humans) according to the classification of the International Agency for Research on Cancer (IARC). DCM is also widely used as an industrial solvent for the purification...
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Clinical studies of detecting COVID-19 from exhaled breath with electronic nose
PublicationThe COVID‑19 pandemic has attracted numerous research studies because of its impact on society and the economy. The pandemic has led to progress in the development of diagnostic methods, utilizing the polymerase chain reaction (PCR) as the gold standard for coronavirus SARS‑CoV‑2 detection. Numerous tests can be used at home within 15 min or so but of with lower accuracy than PCR. There is still a need for point‑of‑care tests available...
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Krzysztof Cwalina dr inż.
PeopleKrzysztof Kamil Cwalina w 2013 r. uzyskał tytuł inżyniera na Wydziale Elektroniki, Telekomunikacji i Informatyki Politechniki Gdańskiej; w 2014 r. uzyskał tytuł magistra inżyniera, a w 2017 r. otrzymał stopień doktora nauk technicznych w dyscyplinie: telekomunikacja, także na WETI PG. Aktualnie pracuje na stanowisku adiunkta w Katedrze Systemów i Sieci Radiokomunikacyjnych Wydziału Elektroniki, Telekomunikacji i Informatyki Politechniki...
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The estimation of total volatile organic compounds emissions generated from peroxide cured natural rubber/polycaprolactone blends
PublicationNatural rubber (NR)/polycaprolactone (PCL) blends at the ratio of 90/10% wt. (NR/PCL90/10) and 70/30% wt. (NR/PCL70/30), with a constant amount of dicumyl peroxide, were prepared by compounding in an internal mixer. Obtained NR/PCL bio-based blends were cured at three different temperatures (150 °C, 160 °C and 170 °C). The total content of volatile organic compounds (TVOC) as a function of the NR/PCL blends ratio, and their curing...
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Structure-property relationships in peroxide-assisted blends of poly(ε-caprolactone) and poly(3-hydroxybutyrate)
PublicationPoly(ε-caprolactone) and poly(3-hydroxybutyrate) (PCL/PHB) blends in two weight ratios (75/25 and 50/50) were reactively compatibilized in the presence of di-(2-tert-butyl-peroxyisopropyl)-benzene and dicumyl peroxide as free radical initiators. Rheological, mechanical, thermal properties and morphological features, as well as the chemical structure of PCL/PHB blends were investigated. It was found that regardless of PCL/PHB blend...
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ENZO-BIO-PUR Sustainable polyurethanes: from cradle to grave with the help of enzymes
ProjectsProject realized in Department of Polymers Technology according to UMO-2023/05/Y/ST8/00076 agreement
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The contingent nature of warehouse flexibility
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Tadeusz Falencikowski dr hab. inż.
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Rapid Multi-band Patch Antenna Yield Estimation Using Polynomial Chaos-Kriging
PublicationYield estimation of antenna systems is important to check their robustness with respect to the uncertain sources. Since the Monte Carlo sampling-based real physics simulation model evaluations are computationally intensive, this work proposes the polynomial chaos-Kriging (PC-Kriging) metamodeling technique for fast yield estimation. PC-Kriging integrates the polynomial chaos expansion (PCE) as the trend function of Kriging metamodel...
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Molecular detection of Candida krusei
PublicationThe species identification of fungi belonging to genus Candida is an important issue as this genus becomes the emerging problem of nosocomial infections. As Candida krusei presents intrinsicresistance to the fluconazole that is the drug of the first choice in case of invasive candidiasis the PCR identifying the DNA of C. krusei was elaborated. The analytical sensitivity of the assay on spiked blood samples was estimated at 3-5...
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Isolation and identification of Mycobacterium avium subspecies silvaticum from a horse
PublicationPo raz pierwszy opracowano metodę identyfikacji Mycobacterium avium subsp. silvaticum na podstawie analizy sekwencji DNA alfa antygenu. Zastosowanie enzymu HhaI do trawienia produktu PCR z uzyskanego z udziałem starterów specyficznych wobec genu kodującego alfa antygen pozwoliło na identyfikację Mycobacterium avium subsp. silvaticum. Opracowana nowa metoda pozwala na prowadzenie badań epidemiologicznych bezpośrednio z próbek pochodzących...
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Expression of cyclin D1 after treatment with doxorubicin in the HL-60 cell line
PublicationIncreased levels of cyclin D1 and amplification of CCND1 gene occur in many types of cancers. We have followed the expression of cyclin D1 after treatment with doxorubicin with reference to cell death and other possible therapeutic implications. The effect of the treatment on the cell cycle, survival, intracellular level (flow cytometry), and intracellular localization of cyclin D1 (fluorescence microscopy) and expression of...
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Evaluation of La 2 Ni 0.5 Cu 0.5 O 4+δ and Pr 2 Ni 0.5 Cu 0.5 O 4+δ Ruddlesden-Popper-type layered oxides as cathode materials for solid oxide fuel cells
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Preliminary studies on the effect of feed speed on the colour change of wood
PublicationThis paper presents the results of preliminary analyses of the effect of cutting parameters on changes in the colour of wood. Beech wood cut with use circular saw was analysed. The cutting parameter tested was the feed speed, represented by the feed per tooth. Sawing processes with different feed per tooth ranging from 0.0008 mm to 0.09 mm were analysed. It was observed that over the entire range of feed rate per tooth analysed,...
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Witold Lewandowski prof. dr hab. inż.
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Possibilities and benefits of a new method of modifying conventional building materials with phase-change materials (PCMs)
PublicationThe paper presents a current study devoted to the optimization of the amount of PCM present within traditional building materials, utilized in this case in hollow-brick walls. Authors focus on proposing an alternative to typical direct addition of PCM to existing building materials: introducing two- and three-component mixtures, based on a porous PCM carrier, which in this case was construction aggregate. Preliminary studies of...
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Jerzy Łabanowski prof. dr hab. inż.
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Effects of Post-Harvest Elicitor Treatments with Ultrasound, UV- and Photosynthetic Active Radiation on Polyphenols, Glucosinolates and Antioxidant Activity in a Waste Fraction of White Cabbage (Brassica oleracea var. capitata)
PublicationBiosynthesis of phytochemicals in leaves of Brassica can be initiated by abiotic factors. The aim of the study was to investigate elicitor treatments to add value to waste of cabbage. A leaf waste fraction from industrial trimming of head cabbage was exposed to UV radiation (250–400 nm, 59 and 99 kJ m-2, respectively), photosynthetic active radiation (PAR, 400–700 nm, 497 kJ m-2), and ultrasound in water bath (35 kHz, at 15, 30...
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Paweł Burdziakowski dr inż.
PeoplePaweł Burdziakowski, PhD, is a professional in low-altitude aerial photogrammetry and remote sensing, marine and aerial navigation. He is also a licensed flight instructor and software developer. His main areas of interest are digital photogrammetry, navigation of unmanned platforms and unmanned systems, including aerial, surface, underwater. He conducts research in algorithms and methods to improve the quality of spatial measurements...
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Novel Approaches of Using of Spirulina Platensis in Natural Rubber Based Composites
PublicationThe aim of this work was to investigate the influence of Spirulina (Spirulina platensis) as a natural filler on the curing characterization, morphology and mechanical, thermomechanical and thermal properties of natural rubber (NR) based composites. Spirulina was introduced into NR mixture in amount of 0 phr, 10 phr and 30 phr. The vulcanization process was carried out at the determined process condition by using hydraulic press...
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On the Correlation of Lignocellulosic Filler Composition with the Performance Properties of Poly(ε-Caprolactone) Based Biocomposites
PublicationIn this work, three types of agricultural waste: olive stones (OS), date seed (DS) and wheat bran (WB) were applied as potential lignocellulosic fillers in poly(ε-caprolactone) (PCL) based biocomposites. Differences in composites’ performance were related to the higher content of proteins, noted for WB comparing to other fillers applied, which enhanced plasticization of PCL matrix. The mechanical properties of biocomposites were...
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Typology of low developed non-metropolitan sub-regions in the European Union
PublicationThis paper shows a typology of 319 NUTS-3 sub-regions in the European Union. The selected sub-regions are non-metropolitan sub-regions, whose gross domestic product per capita in 2011 was below 75% of the EU average. The objective of this typology was to recognize diversity in the examined sub-regions as a starting point for the selection of areas for future comparative research. The typology takes into account 24 variables related...
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Analiza sterowania ułamkowego PIλDμ mocą reaktora jądrowego
PublicationW artykule przedstawiono syntezę regulatora PIλDμ niecałkowitego rzędu dla potrzeb sterowania mocą reaktora jądrowego lekko wodnego określanego, jako typu PWR (Pressurized Water Reactor). W tym celu wykorzystano nieliniowy model matematyczny reaktora PWR o parametrach skupionych obejmujący procesy generacji i wymiany ciepła oraz termicznych efektów reaktywnościowych. Nastawy regulatora PIλDμ niecałkowitego rzędu dobrano w sposób...
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Post-Pyrolytic Carbon as a Phase Change Materials (PCMs) Carrier for Application in Building Materials
PublicationThis article covers new application for char as a carrier of phase-change materials (PCM) that could be used as an additive to building materials. Being composed of bio-char and PCM, the granulate successfully competes with more expensive commercial materials of this type, such as Micronal® PCM. As a PCM carrier, char that was obtained from the pyrolysis of chestnut fruit (Aesculus hippocastanum) with different absorbances of the...
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Metody Numeryczne w AiR - laboratorium 2022
e-Learning CoursesACR, stopień I, semestr 6Prowadzący: dr inż. Mariusz Domżalski
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Metody Numeryczne w AiR - laboratorium 2023
e-Learning CoursesACR, stopień I, semestr 6Prowadzący: dr inż. Mariusz Domżalski
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Understanding the Dominant Physics Mechanisms on the p-i-n Perovskite Solar Cells Fabricated by Scalable Slot-Die Coating Process in Ambient Air
PublicationPerovskite solar cells (PSC) are emerging technologies that have shown continuous improvement in power conversion efficiency (PCE) and stability. However, a very important aspect that has been seldom considered is the reproducibility of PCE of PSC devices. It is possible to achieve PCE from 10.21% to 17.05% using scalable slot-die-coating technique. However, a spatial distribution of performance is clearly observed for device samples...
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Thermal properties of a cement composite containing phase change materials (PCMs) with post-pyrolytic char obtained from spent tyres as a carrier
PublicationThis work examines the possible use of post-pyrolysis char made from spent vehicle tyres as a carrier for phase change materials (PCM), which could be used as an additive to cement mortar. Because of the ability of PCM to accumulate heat, the cement composite obtained from it, apart from its structural and strength properties, will acquire an additional energy storage function, which can be very important for energy-saving construction. In...
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Podstawy Sterowania Komputerowego 2023
e-Learning CoursesACR/KSDiR, stopień I, semestr 6Prowadzący: dr inż. Mariusz Domżalski