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Search results for: SERINE PROTEINASE
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Game-based Sprint retrospectives: multiple action research
PublicationIn today’s fast-paced world of rapid technological change, software development teams need to constantly revise their work practices. Not surprisingly, regular reflection on how to become more effective is perceived as one of the most important principles of Agile Software Development. Nevertheless, running an effective and enjoyable retrospective meeting is still a challenge in real environments. As reported by several studies,...
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Marine autonomous surface ship - control system configuration
PublicationThis paper addresses the problem of marine autonomous surface ship (MASS) control. The contribution of the paper is the development of a control system configuration, done assuming fully autonomous MASS operation under distinct operational conditions. The overview of hardware and software selection is included.
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SERVICE ORIENTED SCENARIOS FOR SUPPORT ENTERPRISE PROJECT MANAGEMENT
PublicationIn the paper, a service oriented architecture is used to describe various scenarios of human-oriented enterprises. They can be modeled by BPMN to show basic information flow. We consider business scenarios to present another modeling solution called service orchestration or service mashup. That approach gives an opportunity for the right management through taking the best possible choice of services, or composition of services....
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Project- and service oriented thinking in engineering management education
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APPLICABILITY OF INLET AIR FOGGING TO MARINE GAS TURBINE
PublicationThe dependency of marine gas turbine on the ambient temperature leads to a decrease of the gas turbine power output in arid areas. Very often gas turbine power output demand is high and the power margins originally designed into the driver , has been exhausted. In such circumstances the inlet air fogging is an effective compensation of gas turbine power. In this paper an analysis of inlet air fogging applicability to marine gas...
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Origin and fate of nanoparticles in marine water – Preliminary results
PublicationThe number, morphology and elemental composition of nanoparticles (<100 nm) in marine water was investigated using Variable Pressure Scanning Electron Microscopy (VP-SEM) and Energy-dispersive X-ray spectroscopy (EDS). Preliminary research conducted in the Baltic Sea showed that the number of nanoparticles in seawater varied from undetectable to 380 (x102) cm-3. Wind mixing and density barriers (thermocline) had a significant impact...
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Mashup Approaches for Improving Service Oriented Business Scenarios
PublicationTwo kinds of business scenarios are considered: application- and service-oriented. It is shown that the former are more flexible and easier reconfigurable for both traditional (human-oriented) and IT services. Moreover, complex IT services can be created by simple IT services using the modern mashup technology. It is the reason why such business scenarios can be modeled by BPMN in much easier ways. A simple example of a supply...
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Pre-swirl energy saving device in marine application
PublicationThis paper covers topics of energy saving device (ESD) with application to marine propulsors. The form of ESD, considered in this paper, consists of fixed lifting foils mounted in front of the screw propeller (the pre-swirl stator/guide vanes). An algorithm for designing propulsion systems, consisting of guide vanes and screw propeller, is presented. The proposed method relies on hybrid lifting line (guide vanes)-lifting surface...
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Algorithms of fast service restoration in survivable multilayer networks
PublicationGłównym celem rozprawy jest zaproponowanie nowych modeli/algorytmów doboru tras zapewniających przeżywalność usług w sieciach IP-MPLS/WDM poprzez zastosowanie wyznaczonych zawczasu ścieżek zabezpieczających. Zakres pracy obejmuje: dokonanie przeglądu mechanizmów transmisji informacji w warstwowych sieciach teleinformatycznych IP-MPLS/WDM; szczegółowy opis sposobów zapewniania przeżywalności usług; zaproponowanie nowych metod doboru...
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Selected problems on application of steel sandwich panels to marine
PublicationW pracy przedstawiono wybrane problemy stosowania stalowych laserowo spawanych paneli w konstrukcji statku. Problemy te wynikają ze specyfiki cienkiej, spawanej laserowo konstrukcji stalowej i obejmują szerokie spektrum zagadnień począwszy od własności wytrzymałościowychy elementarnego połączenia teowego spawanego laserowo poprzez łączenie paneli ze sobą a skończywszy na integracji paneli z konstrukcją tradycyjną.
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Determining QoS in the Video Telephony Service in an IP Environment
PublicationIP networks are indispensable nowadays. They are among the most efficient platforms. The constantly growing number of users and new services in these networks - the largest being the Internet - requires a good quality of any application used.Determining the QoS in real-time services is particularly important. This work is dedicated to exactly this aspect of the real-time service Video Telephony over IP (VToIP). First, the ITU-T...
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Web-based GIS as a tool for supporting marine research
PublicationZbieranie danych morskich podczas badań hydroakustycznych jest złożonym zadaniem, na które składają się nie tylko pomiary, ale także analiza, weryfikacja i interpretacja informacji uzyskanych przez różne czujniki. Automatyczna integracja, wizualizacja i przetwarzanie zgromadzonych danych pozwala na bardziej precyzyjne rozpoznanie badanych zjawisk. W pracy przedstawiono dedykowany sieciowy system syntezy i rozpowszechniania danych...
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Structural pounding models with Hertz spring and nonlinear damper
PublicationW artykule przedstawiono porównanie możliwości wykorzystania dwóch modeli zderzeń: nieliniowego modelu lepkosprężystego i modelu Hertz'a z dodatkowym tłumieniem. Efektywność każdego z modeli zbadano porównując wyniki analiz numerycznych z wynikami badań eksperymentalnych. Wyniki pracy pokazują, iż zastosowanie nieliniowego modelu lepkosprężystego prowadzi do mniejszych błędów w przebiegach czasowych siły zderzenia oraz do mniejszych...
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Accuracy analysis of the radio location service in the sea environment
PublicationW artykule przedstawiono koncepcję systemu bezpieczeństwa na morzu w oparciu o istniejące systemy komórkowe. Scharakteryzowano problem lokalizowania terminala ruchomego przez sieć w warunkach morskich. Przedstawiono wyniki badań przeprowadzonych w warunkach rzeczywistych w odniesieniu do efektywności lokalizowania terminala na morzu. Przedstawiono wnioski, wypływające z przeprowadzonych analiz.
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Experimental tests of water lubricated marine rubber bearing
PublicationW referacie przedstawiono stosowane obecnie rodzaje łożysk gumowych smarowanych wodą. Omówiono ich zalety i wady.W drugiej części pracy przedstawiono wyniki badań eksperymantalnych typowego okrętowego łożyska gumowego. Uzyskane rezultaty porównano z wynikami badań łożysk z polimerową panwią.
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Strategie wzrostowe dla Neptun Ship Service Ltd.
PublicationW ekspertyzie przeprowadzono ewaluację strategii wzrostowych dla firmy Neptun Ship Service Ltd.
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How to provide fair service for V2I communications in VANETs?
PublicationIn this paper, we focus on fairness issues of Vehicle-to-Infrastructure (V2I) communications. In particular, we show that under a common technique of selection of RSUs by OBUs based on the received signal strength, a vast variability of a number of OBUs connected to RSUs can be observed leading to inefficient/unfair service provided by RSUs. To overcome this problem, we propose an algorithm for RSU selection called RSEL to obtain...
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Radio Link Measurement Methodology for Location Service Applications
PublicationThe aim of this paper is the methodology of measurements executed in a radio link for the realization of radiolocation services in radiocommunication networks, particularly in cellular networks. The main results of the measurements obtained in the physical layer of the universal mobile telecommunications system (UMTS) are introduced. A new method for the utilization of the multipath propagation phenomenon to improve the estimation...
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Comparison of EHD devices with parallel and in series spiked electrodes
PublicationIn this paper two electrohydrodynamic (EHD) devices for gas pumping and cleaning are presented. In both cases to induce an airflow in these EHD devices corona discharge was used. The discharge was generated between the spiked electrodes set parallel (the first case) or in series (the second case) and the plate electrodes. An asymmetric electric field and generated discharge result in unidirectional gas flow through the EHD device....
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Commonly Accessible Web Service Platform - Wiki-WS
PublicationWeb Service technology on the basis had to supply complete and reliable system components. Nowadays this technology is commonly used by companies providing results of their work to end users and hiding implementation details. This paper presents a SOA-enabled platform - Wiki-WS - that empowers users to deploy, modify, discover and invoke web services. Moreover it discusses concepts and functionalities of this open source management...
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Quantum issues in diagnostics of marine energy machines and devices
PublicationW artykule podjęto zagadnienie możliwości uwzględniania części dorobku mechaniki kwantowej w diagnostyce okrętowych urządzeń energetycznych. Wykazano, że są zbieżne spostrzeżenia mechaniki kwantoweji diagnostyki technicznej uzyskane w obszarze wiedzy z zakresu badań empirycznych. Uzasadniono konieczność wykonywania podczas badań empirycznych, nie tylko rachunku błędów, lecz także określania niedokładności wyników badań (pomiarów)...
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Marine Fuel Sulphur Limit Impact on Air Pollution
PublicationThe article presents calculation of Sulphur oxides percentage drop rate in marine industry recorded after 01.01.2020 when new limits, provided by International Maritime Organizations legislation, became effective. Ships’ SOx global emission was estimated and compared between 4th quarter 2019 and 1st quarter 2020. For more accurate estimation 3 seaside cities with big harbors were selected for statistical analysis. Noticeable...
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Marine polymers in tissue bioprinting: Current achievements and challenges
PublicationBioprinting has a critical role in tissue engineering, allowing the creation of sophisticated cellular scaffolds with high resolution, shape fidelity, and cell viability. Achieving these parameters remains a challenge, necessitating bioinks that are biocompatible, printable, and biodegradable. This review highlights the potential of marine-derived polymers and crosslinking techniques including mammalian collagen and gelatin along...
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Video of LEGO Bricks on Conveyor Belt Dataset Series
PublicationThe dataset series titled Video of LEGO bricks on conveyor belt is composed of 14 datasets containing video recordings of a moving white conveyor belt. The recordings were created using a smartphone camera in Full HD resolution. The dataset allows for the preparation of data for neural network training, and building of a LEGO sorting machine that can help builders to organise their collections.
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Experience marketing in the service of the “secular religion” of big tech
PublicationThe article proposes an interpretation of the meaning of experience marketing as a tool in the process of sacralisation of products, and in a broader context – the so-called secular religion accompanying some contemporary big tech organizations. The article is of theoretical nature and the authors have intended to explain how big tech corporations, in line with the concept of experience marketing (digital platforms, in particular),...
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Performance Models of a Multidomain IMS/NGN Service Stratum
PublicationThis paper quantifies call processing performance (CPP) of a multidomain IMS/NGN architecture, which was proposed to deliver current and future telecommunication services with strict quality requirements, independently of the transport network technologies. A realistic simulation model is used as a reference for evaluation of the analytical results, in which vari-ous types of queuing systems are applied to model the opera-tion...
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Minimising the Churn Out of the Service by Using a Fairness Mechanism
PublicationThe paper proposes an algorithm of bandwidth distribution, ensuring fairness to end-users in computer networks. The proposed algorithm divides users into satisfied and unsatisfied users. It provides fairness in terms of quality of experience (QoE) for satisfied users and quality of service (QoS) for unsatisfied users. In this paper, we present detailed comparisons relevant to service providers to show the advantages of the proposed...
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Guitar String Sound Retrieved from Moving Pixels
PublicationThe aim of this study was to develop a method of visual recording and analyzing the vibrations of guitar strings using high-speed cameras and dedicated video processing algorithms. The recording of a plucked string reveals the way in which the deformations propagate, composing the standing and travelling wave. The paper compares the results for a few selected models of classical and acoustic guitars, and it involves processing...
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Providing Differentiated Levels of Service Availability in VANET Communications
PublicationInter-vehicle communications seems to be a promising remedy not only for a number of road-safety issues, but also to improve the efficiency of road traffic, as well as to support the on-board infotainment applications. However, it often encounters disruptions due to high mobility of vehicles causing frequent failures of communication links. In this paper, we utilize the idea of multipath vehicle-to-vehicle routing to provide protection...
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Low Cost Method for Location Service in the WCDMA System
PublicationA new and low cost method for a location service (LCS) in the Wideband Code Division Multiple Access (WCDMA) system is outlined. This method, which is called TDOA + RTT, enables calculation of the geographical position of a mobile station (MS) without knowledge of relative time differences (RTDs) between base stations (BSs). The TDOA+RTT method is based on the measurement of round trip times (RTTs) between the MS and the serving...
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End-to-end service survivability under attacks on networks
PublicationW artykule zaproponowano model znajdowania przeżywalnych i odpornych na ataki połączeń od końca do końca dla sieci o topologii bezskalowej. Głównymi założeniami są: zróżnicowanie sposobu zapewniania przeżywalności połączeń w zależności od klasy ruchu oraz omijanie przez ścieżki aktywne węzłów, narażonych na ataki (tzw. centrów). Wprowadzono nową metrykę dla wyznaczania ścieżek, proporcjonalną do wymaganej jakości obsługi połączeń,...
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Virtual screening for small molecular non-covalent binders of the SARS-CoV-2 main protease
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Efficient removal of dyes and proteins by nitrogen-doped porous graphene blended polyethersulfone nanocomposite membranes
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Investigations of changes in the arabinogalactan proteins (AGPs) structure, size and composition during the fruit ripening process
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Ovocystatin Induced Changes in Expression of Alzheimer’s Disease Relevant Proteins in APP/PS1 Transgenic Mice
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Protective Effect of Black Tea Against Ethanol-Induced Oxidative Modifications of Liver Proteins and Lipids
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Discrimination of normal oral mucosa from oral cancer by mass spectrometry imaging of proteins and lipids
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Lon Protease Is Important for Growth under Stressful Conditions and Pathogenicity of the Phytopathogen, Bacterium Dickeya solani
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Extracellular Matrix Proteins Expression Profiling in Chemoresistant Variants of the A2780 Ovarian Cancer Cell Line
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Prediction of the structures of proteins with the UNRES force field, including dynamic formation and breaking of disulfide bonds
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How proteins bind to DNA: target discrimination and dynamic sequence search by the telomeric protein TRF1
PublicationTarget search as performed by DNA-binding proteins is a complex process, in which multiple factors contribute to both thermodynamic discrimination of the target sequence from overwhelmingly abundant off-target sites and kinetic acceleration of dynamic sequence interrogation. TRF1, the protein that binds to telomeric tandem repeats, faces an intriguing variant of the search problem where target sites are clustered within short fragments...
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Amides as models to study the hydration of proteins and peptides — spectroscopic and theoretical approach on hydration in various temperatures
PublicationInteractions with water are one of the key factors which determine protein stability and activity in aqueous solutions. However, the protein hydration is still insufficiently understood. N-methylacetamide (NMA) is regarded as a minimal part of the peptide backbone and the relative simplicity of its structure makes it a good model for studies on protein–water interactions. In this paper, the influence of NMA and N,N-dimethylacetamide...
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Human sperm proteins identified by 2-dimensional electrophoresis and mass spectrometry and their relevance to a transcriptomic analysis
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Two highly thermostable paralogous single-stranded DNA-binding proteins from Thermoanaerobacter tengcongensis
PublicationThe thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. coli overexpression systems. The TteSSB2 and TteSSB3 consist of 153 and 150 amino acids with a calculated molecular...
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Identification and properties of the Deinococcus grandis andDeinococcus proteolyticus single-stranded DNA binding proteins (SSB)
PublicationW celu poznania właściwości biochemicznych białek SSB pochodzących z Deinococcus grandis (DgrSSB) i Deinococcus proteolyticus (DprSSB) sklonowano zaamplifikowane w reakcji PCR geny ssb w systemie ekspresyjnym Escherichia coli. Geny składają się z 891(DgrSSB) i 876 (DprSSB) nukleotydów kodujących odpowiednio 296 i 291 reszt aminokwasowych o teoretycznie wyznaczonej masie cząsteczkowej dla białek, równej 32,29 i 31,33 kDa. Sekwencja...
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Bacterial SSB-like proteins of family Deinococcaceae - indentification, isolation, obtaining, purification and molecular characteristic
PublicationWiększość poznanych białek SSB jest aktywna w formie homotetramerycznej. Od 2002 roku znane są białka organizmów ekstremofilnych. Scharakteryzowano białka mikroorganizmów rodziny Deinococaceae. Uzyskane termostabilne białka stanowią atrakcyjną alternatywę w stosunku do znanych białek SSB w metodach diagnostyki molekularnej, technikach biologii molekularnej i analityce.
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The Immunogenic and Immunoprotective Activities of Recombinant Chimeric T. gondii Proteins Containing AMA1 Antigen Fragments
PublicationToxoplasmosis, one of the most common parasitoses worldwide, is potentially dangerous for individuals with a weakened immune system, but specific immunoprophylaxis intended for humans is still lacking. Thus, efforts have been made to create an efficient universal vaccine for both animals and humans to overcome the shortcomings of currently used treatment methods and protect all hosts against toxoplasmosis. The current work represents...
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Unraveling the Interplay between DNA and Proteins: A Computational Exploration of Sequence and Structure-Specific Recognition Mechanisms
PublicationMy PhD dissertation focused on DNA-protein interactions and the recognition of specific DNA sequences and structures. I discovered that acidic amino acid residues (Asp/Glu) play a crucial role by exhibiting a preference for cytosine. Their contribution to binding affinity depends on nearby cytosines, balancing electrostatic repulsion with specific interactions. Acidic residues act as negative selectors, discouraging non-cytosine...
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The biological role of prolyl oligopeptidase and the procognitive potential of its peptidic inhibitors from food proteins
PublicationProlyl oligopeptidase (POP) is a conserved serine protease belonging to proline-specific peptidases. It has both enzymatic and non-enzymatic activity and is involved in numerous biological processes in the human body, playing a role in e.g., cellular growth and differentiation, inflammation, as well as the development of some neurodegenerative and neuropsychiatric disorders. This article describes the physiological and pathological...
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Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus-expression and purification
PublicationSklonowano w system ekspresyjny pET-30LIC Escherichia coli gen kodujący białka SSB-podobne Thermus thermophilus i T. aquaticus. Oczyszczono do homogenności białka SSB-podobne, a uzyskane preparaty umożliwiają wykonanie badań strukturalnych w/w białek.