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Wyniki wyszukiwania dla: lens-less microscopy
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Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms
PublikacjaA significant number of DNA-based techniques has been introduced into the field of microorganisms’ characterization and taxonomy. These genomic fingerprinting methods were developed to detect DNA sequence polymorphisms by using general principles, such as restriction endonuclease analysis, molecular hybridization, and PCR amplification. In recent years, some alternative techniques based on ligation of oligonucleotide adapters before...
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Importance of the combined effects of dissolved oxygen and pH on optimization of nitrogen removal in anammox-enriched granular sludge
PublikacjaThe combined effects of dissolved oxygen (DO) and pH on nitrogen removal were investigated in a laboratory-scale sequencing batch reactor (SBR) with anammox-enriched granular sludge obtained from a nitritation/anammox system. The highest specific nitrogen removal rate (SNRR) (1.1 gN gVSS−1 d−1) was observed under non-aerated conditions, resulting in the nitrogen removal efficiency of 81.6%. Although nitrogen removal was readily...
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Conservation and diversity of MutS proteins
PublikacjaThe homologues of MutS, mismatch repair protein, exist in all prokaryotes, with the exception of Actinobacteria, Mollicutes and part of the Archaea. Multiple alignments of 316 MutS amino acid sequences from 169 species revealed conserved residues and sequence motifs distinguishing MutS homologues. All MutS homologues show high conservation within the ATPase domain. MutS1, the homologue responsible for DNA mismatch recognition,...
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Revisiting The Determination Of Cutting Power While Sawing Of Wood With Circular Saw Blades By Means Of Fracture Mechanics
PublikacjaIn the classical approach, energetic effects (cutting forces and cutting power) of wood sawing process are generally calculated on the basis of the specific cutting resistance, which is in the case of wood cutting the function of more or less important factors. On the other hand, cutting forces (power) could be considered from a point of view of modern fracture mechanics. Cutting forces may be employed to determine not only toughness...
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Simple Superstrate Antenna for Connectivity Improvement in Precision Farming Applications
PublikacjaIn this paper, a concept of a simple circularly polarized antenna with partially reflecting surface (PRS) has been adopted for precision farming applications. The investigation contains an analysis of the dependence of the antenna performance on the elements number in the PRS structure in X- and Ka-band frequencies. Especially meaningful parameters from point-to-point connectivity perspective are...
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Mass Spectrometric Identification of 13C-labeled Metabolites During anaerobic Propanoic Acid Oxidation
PublikacjaBiowaste digestion is a possibility to gain biogas as a renewable fuel source. However, the anaerobic food chain may be disrupted by, e.g., substrate overload or by inhibitors, leading to the accumulation of volatile fatty acids (VFAs), predominantly of propanoic acid (PA). VFA Accumulation may cause a rapid pH decrease, less biogas production, or even a total inhibition. To maintain high biogas productivity or to prevent a collapse...
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Prediction of metal deformation due to line heating; an alternative method of mechanical bending, based on artificial neural network approach
PublikacjaLine heating is one of the alternative methods of forming metals and this kind of forming uses the heating torch as a source of heat input. During the process, many parameters are considered like the size of the substrate, thickness, cooling method, source power intensity, the travel speed of the power source, the sequence of heating, and so on. It is important to analyze the factors affecting the...
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Caring for Children with Dravet Syndrome: Exploring the Daily Challenges of Family Caregivers
PublikacjaWhile Polish studies focus on the symptoms, causes and treatment of people suffering from Dravet syndrome (DS), much less is known about the situation of the family caregivers of DS children. This study was designed to explore the experiences, daily challenges and needs related to caring for DS children. An anonymous self-administered online questionnaire was developed. Thesurvey was completed by 75 family caregivers affiliated...
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The influence of cultivation conditions on the myrosinase activity and glucosinolate content in white cabbage
PublikacjaIn the process of biofumigation typically natural compounds present in Brassicaceae family are used to combat pests and protect crops. Glucosinolates, sulfur-containing secondary metabolites found in Brassica plants, are hydrolized by the enzyme myrosinase (β-thioglucosidase, EC 3.2.3.1) with the liberation of degradation products such as isothiocyanates, nitriles, thiocyanates and epithionitriles. Isothiocyanates are the most...
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Processing, physico-mechanical and thermal properties of reclaimed GTR and NBR/reclaimed GTR blends as function of various additives
PublikacjaIn this work, ground tire rubber (GTR) was mechano-chemically reclaimed in the presence of bitumen and various additives. During studies three types of processing and curing additives: (i) peptizer P300; (ii) vulcanization accelerator tetramethylthiuram disulfide (TMTD) and (iii) organic peroxide di(2-tert-butyl-peroxyisopropyl)benzene (BIB1) were applied to enhance reclaiming of GTR. Reclaiming process was evaluated by oscillating...
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Impact of overloaded vehicles on load equivalency factors and service period of flexible pavements
PublikacjaOverloaded vehicles occur less frequently in comparison to properly loaded vehicles but due to their greater potential to cause damage they significantly contribute to the distress of pavement structure. As the studies show, the phenomena of vehicles overloading intensifies when the control of traffic is poor and the average percentage of overloaded vehicles on analyzed roads varies from 6% to 23%. The main goal of the ar-ticle...
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Protection and renovation of public areas in a modernist city: the case of three streets in the historic City Centre of Gdynia
PublikacjaThis article concerns issues related to the preservation and restoration of public space in Gdynia’s city centre, which was designed and built mainly in the 1920s and 1930s. In 2007, a major part of the city centre was entered into the register of monuments asa historic complex of urban architecture. Moreover, in 2015, the area was declared a Monument of History. Protection of a complex of urban architecture covers not only the...
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Accurate Lightweight Calibration Methods for Mobile Low-Cost Particulate Matter Sensors
PublikacjaMonitoring air pollution is a critical step towards improving public health, particularly when it comes to identifying the primary air pollutants that can have an impact on human health. Among these pollutants, particulate matter (PM) with a diameter of up to 2.5 μ m (or PM2.5) is of particular concern, making it important to continuously and accurately monitor pollution related to PM. The emergence of mobile low-cost PM sensors...
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Sensitive method for determination of benzoic acid in beverages and food samples using air–assisted hydrophobic deep eutectic solvent-based dispersive liquid-liquid microextraction
PublikacjaA simple, reliable and rapid air–assisted hydrophobic deep eutectic solvent-based dispersive liquid–liquid microextraction (AA-HDES-DLLME) was developed for analysis of benzoic acid in various beverages and food samples. The final determination stage was performed via UV–visible spectrophotometry. The key parameters (extraction time, HDES type and volume, dispersant volume, pH and sample volume) of the AA-HDES-DLLME method were...
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Field Calibration of Low-Cost Particulate Matter Sensors Using Artificial Neural Networks and Affine Response Correction
PublikacjaDue to detrimental effects of atmospheric particulate matter (PM), its accurate monitoring is of paramount importance, especially in densely populated urban areas. However, precise measurement of PM levels requires expensive and sophisticated equipment. Although low-cost alternatives are gaining popularity, their reliability is questionable, attributed to sensitivity to environmental conditions, inherent instability, and manufacturing...
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Uncovering the invisible barriers to women’s success
PublikacjaIn the area of science and higher education, as in business and politics, the job situation of women is improving, and the percentage of women on executive positions is increasing. However, there is still a serious underrepresentation of women in the highest decision-making bodies. Ladies also take part in the strategic institutional events less frequently. There are still serious disproportions in academic and management positions,...
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The Idea of Using Adhesive Bonds in Shaping of Cold-formed Thin-walled Beam-columns
PublikacjaThe chapter concerns the idea of using adhesive bonds in the shaping of cold-formed thin-walled beam-columns. The idea of adhesive-bonded connections is not new but is unlikely to have been used in the design and shaping of thin-walled beam-columns in civil engineering up to date. Metal bar structures, including (due to economic reasons) thin-walled structures, are the most common and effective means to build engineering superstructures/loadbearing...
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Effective assessment of biopolymer-based multifunctional sorbents for the remediation of environmentally hazardous contaminants from aqueous solutions
PublikacjaPersistent contaminants in wastewater effluent pose a significant threat to aquatic life and are one of the most significant environmental concerns of our time. Although there are a variety of traditional methods available in wastewater treatment, including adsorption, coagulation, flocculation, ion exchange, membrane filtration, co-precipitation and solvent extraction, none of these have been found to be significantly cost-effective...
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Polymer derived SiOC/Sn nanocomposites from a low-cost single source precursor as anode materials for lithium storage applications
PublikacjaMetal- based materials capable of lithium (Li) alloy formation are key to realization of the next generation of high-energy density anodes for Li-ion batteries, owing to their high storage capacity. Designing a good sup- porting matrix is essential for homogeneously nesting these metallic nanodomains, to effectively utilize their high capacity while tackling the volume expansion issues. Silicon oxycarbides (SiOC), obtained via...
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The Product of Matrix Metalloproteinase Cleavage of Doxorubicin Conjugate for Anticancer Drug Delivery: Calorimetric, Spectroscopic, and Molecular Dynamics Studies on Peptide–Doxorubicin Binding to DNA
PublikacjaMatrix metalloproteinases (MMPs) are extracellular matrix degradation factors, promoting cancer progression. Hence, they could provide an enzyme-assisted delivery of doxorubicin (DOX) in cancer treatment. In the current study, the intercalation process of DOX and tetrapeptide-DOX, the product of the MMPs' cleavage of carrier-linked DOX, into dsDNA was investigated using stationary and time-resolved fluorescence spectroscopy, UV-Vis...
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Arching of railway turnouts by analytical design method
PublikacjaThe paper involved the issue of arching of the railway turnouts. This is an issue which is given relatively less attention to scientific and research activities. Reference has been made to the book by Wladyslaw Rzepka, under the title „Curved turnouts in plan and profile”, which has been used in Poland for more than 50 years as the main source of information relating to the turnouts on the curve. The book is a compilation of elaborations...
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Investigation of the Antifungal Activity and Mode of Action of Thymus vulgaris, Citrus limonum, Pelargonium graveolens, Cinnamomum cassia, Ocimum basilicum, and Eugenia caryophyllus Essential Oils
PublikacjaThe antimicrobial activity of plant oils and extracts has been recognized for many years. In this study the activity of Thymus vulgaris, Citrus limonum, Pelargonium graveolens, Cinnamomum cassia, Ocimumbasilicum, and Eugenia caryophyllus essential oils (EOs) distributed by Pollena Aroma (Nowy Dwór Mazowiecki, Poland) was investigated against a group of 183 clinical isolates of C. albicans and 76 isolates of C. glabrata. All of...
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Deep neural networks for human pose estimation from a very low resolution depth image
PublikacjaThe work presented in the paper is dedicated to determining and evaluating the most efficient neural network architecture applied as a multiple regression network localizing human body joints in 3D space based on a single low resolution depth image. The main challenge was to deal with a noisy and coarse representation of the human body, as observed by a depth sensor from a large distance, and to achieve high localization precision....
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Analytical design method for widening the intertrack space
PublikacjaThe paper involved the issue of arching of the railway turnouts. This is an issue which is given relatively less attention to scientific and research activities. Reference has been made to the book by Wladyslaw Rzepka, under the title „Curved turnouts in plan and profile”, which has been used in Poland for more than 50 years as the main source of information relating to the turnouts on the curve. The book is a compilation of elaborations...
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Surgical Site Infection after Breast Surgery: A Retrospective Analysis of 5-Year Postoperative Data from a Single Center in Poland
PublikacjaBackground and Objectives: Surgical site infection (SSI) is a significant complication of non-reconstructive and reconstructive breast surgery. This study aimed to assess SSI after breast surgery over five years in a single center in Poland. The microorganisms responsible for SSI and their antibiotic susceptibilities were determined. Materials and Methods: Data from 2129 patients acquired over five years postoperatively by the...
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The Central European GNSS Research Network (CEGRN) dataset
PublikacjaThe Central European GNSS Research Network (CEGRN) collects GNSS data since 1994 from contributors which today include 42 Institutions in 33 Countries. CEGRN returns a dataset of coordinates and velocities computed according to international standards and the most recent processing procedures and recommendations. We provide a dataset of 1229 positions and velocities resulting from 3 or more repetitions of coordinate measurements...
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Low temperature broad band dielectric spectroscopy of multiferroic Bi6Fe2Ti3O18 ceramics
PublikacjaIn the present research the tool of broadband dielectric spectroscopy was utilized to characterize dielectric behavior of Bi6Fe2Ti3O18 (BFTO) Aurivillius-type multiferroic ceramics. Dielectric response of BFTO ceramics was studied in the frequency domain (Δν=0.1Hz – 10MHz) within the temperature range ΔT=-100°C – 200°C. The Kramers-Kronig data validation test was employed to validate the impedance data measurements and it was found...
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Permeability of the small intestinal mucus for physiologically relevant studies: Impact of mucus location and ex vivo treatment
PublikacjaThe small intestinal mucus is a complex colloidal system that coats the intestinal mucosa. It allows passage on nutrients/pharmaceuticals from the gut lumen towards the epithelium, whilst preventing it from direct contact with luminal microorganisms. Mucus collected from intestinal tissue is often used in studies looking at inter-mucosal transport of food particulates, drug carriers, etc. However, detaching the highly hydrated...
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Neural network training with limited precision and asymmetric exponent
PublikacjaAlong with an extremely increasing number of mobile devices, sensors and other smart utilities, an unprecedented growth of data can be observed in today’s world. In order to address multiple challenges facing the big data domain, machine learning techniques are often leveraged for data analysis, filtering and classification. Wide usage of artificial intelligence with large amounts of data creates growing demand not only for storage...
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Crystal structures of the DsbG disulfide isomerase reveal an unstable disulfide
PublikacjaDsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. Here, we present two crystal structures of DsbG at 1.7- and 2.0-Angstrom resolution that are meant to represent the reduced and oxidized forms, respectively. The oxidized structure, however, reveals a mixture...
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Linear and Nonlinear Optical Properties of Azobenzene Derivatives Modified with an (Amino)naphthalene Moiety
PublikacjaThe design of two-photon absorbing azobenzene (AB) derivatives has received much attention; however, the two-photon absorption (2PA) properties of bis-conjugated azobenzene systems are relatively less explored. Here, we present the synthesis of six azobenzene derivatives and three bisazobenzenes substituted (or not) at para position(s) with one or two amino group(s). Their linear and nonlinear absorption properties are studied...
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Post-match recovery profile of leukocyte cell subsets among professional soccer players
PublikacjaThis study assessed the impact of cumulative match time on the distribution of CD45+ cell subtests in the capillary blood of professional soccer players. Twenty-two males (aged 18–30 years) took part in the 36-week study. Participants playing up to 540 in cumulative match time and less than 30 min in each single match during the observation period formed the control group. White blood cell (WBC) phenotyping and creatine kinase...
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Interactions between polyphenolic antioxidants quercetin and naringenin dictate the distinctive redox-related chemical and biological behaviour of their mixtures
PublikacjaFood synergy concept is suggested to explain observations that isolated antioxidants are less bioactive than real foods containing them. However, mechanisms behind this discrepancy were hardly studied. Here, we demonstrate the profound impact of interactions between two common food flavonoids (individual: aglycones quercetin—Q and naringenin—N− or their glycosides rutin—R and naringin—N+ vs. mixed: QN− and RN+) on their electrochemical...
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Niobate in silicate and phosphate glasses: Effect of glass basicity on crucible dissolution
PublikacjaUsing niobium crucibles for melting phosphate and silicate glasses of various modifier oxide contents, and therefore varying optical basicity (Λ), was found to result in varying dissolution rates of niobate during melting. Because of their high electronic polarizability, even small concentrations of niobates are detectable in the Raman spectra of glasses. Even less than 1 mol% Nb2O5 can be identified, as independently confirmed...
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Relatively high-Seebeck thermoelectric cells containing ionic liquids supplemented by cobalt redox couple
PublikacjaMeanwhile no general and reliable equation determining the Seebeck coefficient (S e ) and involving electro-chemical reaction effects was derived for solutions. We reported the database of 15,000 ionic liquids supplemented by three different redox couple systems: 0.01 mol/l Co 3+/2+ (bpy) 3 , 0.01 mol/l I 3-/3I- and 0.2 mol/l 3- /3I- , and the corresponding estimated Seebeck coefficients. We also reported methods for estimating...
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Potential reduction of traffic noise by the means of increased fleet of electric vehicles using a combination of low-noise tyres and low-noise road surfaces
PublikacjaIn the future, the number of zero-emission vehicles like electric and plug-in hybrid vehicles (in electric mode) is expected to be a substantial part of the vehicle fleet. In Norway, such vehicles already account for approximately 20 % of all new cars sold. Since these vehicles emit negligible noise related to the power-train, the tyre/road noise is the dominating noise source. In the LEO project, tyres designed for such cars have...
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Implementing Sustainable Development Goals with Digital Government – Aspiration-capacity gap
PublikacjaSustainable Development Goals (SDGs) represent a commitment by all United Nations Member States to pursue development efforts, including ending poverty and hunger, promoting well-being and education, reducing inequalities, fostering peace, and protecting the planet. Member States and their governments are supposed to take ownership of the SDGs, strengthen the implementation means, and improve public governance as both the means...
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Ni/cerium Molybdenum Oxide Hydrate Microflakes Composite Coatings Electrodeposited from Choline Chloride: Ethylene Glycol Deep Eutectic Solvent
PublikacjaCerium molybdenum oxide hydrate microflakes are codeposited with nickel from a deep eutectic solvent-based bath. During seven days of exposure in 0.05 M NaCl solution, the corrosion resistance of composite coating (Ni/CeMoOxide) is slightly reduced, due to the existence of some microcracks caused by large microflakes. Multielemental analysis of the solution, in which coatings are exposed and the qualitative changes in the surface...
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Structure of Microemulsion Formulated with Monoacylglycerols in the Presence of Polyols and Ethanol
PublikacjaThe influence of polyols as cosurfactants (propylene glycol PG; glycerol G) and short chain alcohol as a cosolvent (ethanol EtOH) on the formation and solubilization capacity of the systems: hexadecane/monoacylglycerols (MAG)/polyol/water:EtOH, at 60C, was investigated. Electrical conductivity measurement, and the DSC method were applied to determine the structure and type of microemulsions formed. The dimension of the droplets...
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Perfectly Wetting Mixtures of Surfactants from Renewable Resources: The Interaction and Synergistic Effects on Adsorption and Micellization
PublikacjaThis paper presents a study of the surface properties of mixtures of surfactants originating from renewable sources, i.e., alkylpolyglucoside (APG), ethoxylated fatty alcohol (AE), and sodium soap (Na soap). The main objective was to optimize the surfactant ratio which produces the highest wetting properties during the analysis of the solution of the individual surfactants, twoand three-component mixtures, and at different pH values....
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Factors determining accumulation of bisphenol A and alkylphenols at a low trophic level as exemplified by mussels Mytilus trossulus
PublikacjaThe aim of the study was to investigate abiotic and biotic factors influencing the accumulation of endocrine disrupting compounds (EDCs) such as bisphenol A (BPA), 4-tert-octylphenol (OP) and 4- nonylphenol (NP) in mussels Mytilus trossulus from the Gulf of Gdansk (Southern Baltic). The key abiotic factor influencing BPA, OP and NP accumulation in mussels is their hydrophilicity/lipophilicity, which affects their main assimilation...
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Polymer based thick films - material quality and interface resistance evaluation
PublikacjaThe properties of polymer based thick film layers mede using different resistive pastes and dipping silvers have been studied. The composite of carbon and graphite (C/Gr) conducting particles suspended in different polymer vehicles were used for preparation resistive layers. Interface resistance Rc created between dipping silver (DiAg) contact layer and resistive layer was determined from the surface potential distribution measurements...
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Professional activity and women entrepreneurship in Poland – Europe 2020 Strategy perspective
PublikacjaThe level of economic activity of women in Poland is the lowest among Baltic Sea Region countries. The analysis of Europe 2020 targets, shows that at least 3 of the 5 main objectives relate, more or less, to women (e.g. participation in labor market). The objective concerning social inclusion assumes the increase in the employment rate of men and women (aged 20-64) in Poland up to 71%. To achieve this goal, support programs to...
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Antioxidant activities and bioactive components in some berries
PublikacjaThe objective of this study was to evaluate the antioxidant and binding effects of gooseberry, a less-stud-ied berry, and to compare with blueberry and cranberry in the model of interaction with human serum albumin (HSA). The relationship between the scavenging properties of dietary polyphenols of the selected berries and their affinities for HSA were investigated by fluorescence anal- ysis. In order to perform the extraction and...
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Economical wood sawing with circular saw blades of a new design (Ekonomicno piljenje drva kruznom pilom novog dezajna)
PublikacjaMaterial-saving and energy-saving belong to the basic requirements imposed on contemporary manufacturing processes. The realization of these processes gives measurable profi ts, not only economical butalso ecological. In the case of wood sawing with circular saw blades, material- and energy-savings are dependent on total overall set of teeth (theoretical kerf) and teeth position accuracy in relation to the workpiece. Hence, it...
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Rozwój Pucka, Kartuz i Nowego Dworu Gdańskiego w latach 2005-2013 = The development of Puck, Kartuzy and Nowy Dwór Gdański In the years 2005-2013
PublikacjaNa terenie województwa pomorskiego znajdują się 42 miasta. Wśród nich 28 to miejscowości o liczbie do 20 000 mieszkańców. Stanowią one 66% wszystkich miast województwa. Według danych GUS na 2006 rok mieszka w nich 16% całkowitej liczby jego miejskiej ludności.W najbliższym sąsiedztwie Gdańska położone są trzy miasteczka o liczbie ludności w przedziale od 10 000 do 20 000. Są nimi: Puck, Kartuzy i Nowy Dwór Gdański. Choć są one...
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Nonlinear secondary arc model use for evaluation of single pole auto-reclosing effectiveness
PublikacjaPurpose – The purpose of this paper is to discuss two evaluation methods of single pole autoreclosing process effectiveness in HV transmission lines. Secondary arc current and recovery voltage results obtained by load flow calculation are compared to the results obtained by the time domain simulations. Moreover, a nonlinear secondary arc implementation is presented. Design/methodology/approach – A computer simulation studies were...
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The importance of trust and altruistic social orientation for COVID-19 distress
PublikacjaBackground: The COVID-19 pandemic, the biggest global health crisis in decades, has been a difficult experience for nations all over the world. In the present study we wanted to assess to what extent a positive attitude towards others, expressed in altruistic social orientation and a high level of trust, would be linked to lower levels of COVID-19 distress in infected and non-infected individuals. Participants and procedure:...
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Ultra-rare ultra-care: The unique burden of ultra rare disease caregiving
PublikacjaBackground: We sought to assesses the impact of caring for children with ultra rare diseases (URDs) on familycarers and to analyse the way these experiences differ among the caregivers of children diagnosed throughprenatal or newborn screening, and those with symptom-based diagnosis.Methods: A total of 200 caregivers of 219 URDs children completed an on-line survey regarding the challengesand experiences...
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Organic-inorganic composites consisted of poly(3,4-ethylenedioxythiophene)and Prussian Blue analogues
PublikacjaThe organic-inorganic material consisted of poly(3,4-ethylenedioxythiophene) (pEDOT) and copper hexacyanoferrate (Cuhcf) was synthesized. The pEDOT film with Fe(CN)63−/4− as counter-ions potentiodynamically polarized in aqueous CuCl2 electrolyte brings about stable hybrid material (pEDOT/Cuhcf) performing single redox activity of FeII/III at a formal potential Ef = 0.61 V (vs. Ag/AgCl/0.1 M KCl) and less clearly shaped two redox...