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Crystal structures of the DsbG disulfide isomerase reveal an unstable disulfide
PublikacjaDsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. Here, we present two crystal structures of DsbG at 1.7- and 2.0-Angstrom resolution that are meant to represent the reduced and oxidized forms, respectively. The oxidized structure, however, reveals a mixture...
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Potential of Silver Nanoparticles in Overcoming the Intrinsic Resistance of Pseudomonas aeruginosa to Secondary Metabolites from Carnivorous Plants
PublikacjaCarnivorous plants are exemplary natural sources of secondary metabolites with biological activity. However, the therapeutic antimicrobial potential of these compounds is limited due to intrinsic resistance of selected bacterial pathogens, among which Pseudomonas aeruginosa represents an extreme example. The objective of the study was to overcome the intrinsic resistance of P. aeruginosa by combining silver nanoparticles (AgNPs)...
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Modelling the time-dependent behaviour of soft soils
PublikacjaTime-dependence of soft soils has already been thoroughly investigated. The knowledge on creep and relaxation phenomena is generally available in the literature. However, it is still rarely applied in practice. Regarding the organic soils, geotechnical engineers mostly base their calculations on the simple assumptions. Yet, as presented within this paper, the rate-dependent behaviour of soft soils is a very special and important...
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Localization of impulsive disturbances in audio signals using template matching
PublikacjaIn this paper, a new solution to the problem of elimination of impulsive disturbances from audio signals, based on the matched filtering technique, is proposed. The new approach stems from the observation that a large proportion of noise pulses corrupting audio recordings have highly repetitive shapes that match several typical “patterns”. In many cases a representative set of exemplary pulse waveforms can be extracted from the...
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Regulated Assembly of LPS, Its Structural Alterations and Cellular Response to LPS Defects
PublikacjaDistinguishing feature of the outer membrane (OM) of Gram-negative bacteria is its asymmetry due to the presence of lipopolysaccharide (LPS) in the outer leaflet of the OM and phospholipids in the inner leaflet. Recent studies have revealed the existence of regulatory controls that ensure a balanced biosynthesis of LPS and phospholipids, both of which are essential for bacterial viability. LPS provides the essential permeability...
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Machine Learning Techniques in Concrete Mix Design
PublikacjaConcrete mix design is a complex and multistage process in which we try to find the best composition of ingredients to create good performing concrete. In contemporary literature, as well as in state-of-the-art corporate practice, there are some methods of concrete mix design, from which the most popular are methods derived from The Three Equation Method. One of the most important features of concrete is compressive strength, which...
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Crystal structures of aminotransferases Aro8 and Aro9 from Candida albicans and structural insights into their properties
PublikacjaAminotransferases catalyze reversibly the transamination reaction by a ping-pong bi-bi mechanism with pyridoxal 5′-phosphate (PLP) as a cofactor. Various aminotransferases acting on a range of substrates have been reported. Aromatic transaminases are able to catalyze the transamination reaction with both aromatic and acidic substrates. Two aminotransferases from C. albicans, Aro8p and Aro9p, have been identified recently, exhibiting...
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Evaluation of citric acid corrosion inhibition efficiency and passivation kinetics for aluminium alloys in alkaline media by means of dynamic impedance monitoring
PublikacjaA novel approach is proposed for constructing the adsorption isotherm in corrosion studies, based on a well-known interaction between citric acid and aluminium in alkaline electrolytes. Our approach utilizes the instantaneous impedance measurements via Dynamic Electrochemical Impedance Spectroscopy in galvanostatic mode (g-DEIS). Unlike other common tools, g-DEIS delivers exact information about the concentration required for full...
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Radioactivity of drilling cuttings from shale resources of the Lower Paleozoic Baltic Basin, Poland
PublikacjaFractionated drilling wastes originating from shale gas exploration in the Baltic Basin in Pomerania were subjected to the measurements of mean activity concentrations of naturally occurring radioactive materials (NORMs).To better understand rock structure and texture, the X-ray diffraction analysis (XRD) and scanning electron microscopy(SEM) observation were used. To compare activity concentration of radionuclides in bulk wastes...
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Dynamic coloring of graphs
PublikacjaDynamics is an inherent feature of many real life systems so it is natural to define and investigate the properties of models that reflect their dynamic nature. Dynamic graph colorings can be naturally applied in system modeling, e.g. for scheduling threads of parallel programs, time sharing in wireless networks, session scheduling in high-speed LAN's, channel assignment in WDM optical networks as well as traffic scheduling. In...
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How to enhance perception of reassembled but incomplete works of ancient art? Eye-tracking study of virtual anastylosis.
Publikacjan 1964 the Venice Charter described anastylosis as the only acceptable method of reassembly of architectural remains. Although the scientific community has agreed with the Charter’s decision, many questions pertaining to the technical and aesthetic aspects of anastylosis remain unanswered. Virtual anastylosis seems one of the most promising digital solutions to finding at least some answers, as it permits testing various solutions...
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Fusion-based Representation Learning Model for Multimode User-generated Social Network Content
PublikacjaAs mobile networks and APPs are developed, user-generated content (UGC), which includes multi-source heterogeneous data like user reviews, tags, scores, images, and videos, has become an essential basis for improving the quality of personalized services. Due to the multi-source heterogeneous nature of the data, big data fusion offers both promise and drawbacks. With the rise of mobile networks and applications, UGC, which includes...
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Case study of water vapour transmission properties of EPDM façade membranes
PublikacjaThis research aimed to investigate the water vapour transmission properties of chosen EPDM membranes applied in façade and window systems under laboratory tests. The applied procedure included in national and international standards utilized for the laboratory tests of water vapour transmission properties of EPDM membrane is described. Two main types (outside and inside types) of EPDM membranes are laboratory tested. The authors...
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The Mechanism of a Retro-Diels–Alder Fragmentation of Luteolin: Theoretical Studies Supported by Electrospray Ionization Tandem Mass Spectrometry Results
PublikacjaThe mechanisms of retro-Diels–Alder fragmentation of luteolin are studied theoretically using the Density Functional Theory method (B3LYP hybrid functional) together with the 6-311++G(d,p) basis set and supported by electrospray ionization tandem mass spectrometry (ESI-MS) results. The reaction paths leading to the formation of 1,3A and 1,3B fragment ions observed as the main spectral features in the ESI-MS spectrum are described...
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Reduced-Cost Two-Level Surrogate Antenna Modeling using Domain Confinement and Response Features
PublikacjaElectromagnetic (EM) simulation tools have become indispensable in the design of contemporary antennas. Still, the major setback of EM-driven design is the associated computational overhead. This is because a single full-wave simulation may take from dozens of seconds up to several hours, thus, the cost of solving design tasks that involve multiple EM analyses may turn unmanageable. This is where faster system representations (surrogates)...
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Assessment of Wide-Sense Stationarity of an Underwater Acoustic Channel Based on a Pseudo-Random Binary Sequence Probe Signal
PublikacjaThe performances of Underwater Acoustic Communication (UAC) systems are strongly related to the specific propagation conditions of the underwater channel. Designing the physical layer of a reliable data transmission system requires a knowledge of channel characteristics in terms of the specific parameters of the stochastic model. The Wide-Sense Stationary Uncorrelated Scattering (WSSUS) assumption simplifies the stochastic description...
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Increasing the Geometrical and Interpretation Quality of Unmanned Aerial Vehicle Photogrammetry Products Using Super-Resolution Algorithms
PublikacjaUnmanned aerial vehicles (UAVs) have now become very popular in photogrammetric and remote-sensing applications. Every day, these vehicles are used in new applications, new terrains, and new tasks, facing new problems. One of these problems is connected with flight altitude and the determined ground sample distance in a specific area, especially within cities and industrial and construction areas. The problem is that a safe flight...
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Limiting distribution of the three-state semi-Markov model of technical state transitions of ship power plant machines and its applicability in operational decision-making.
PublikacjaThe article presents the three-state semi-Markov model of the process {W(t): t 0} of state transitions of a ship power plant machine, with the following interpretation of these states: s1 – state of full serviceability, s2 – state of partial serviceability, and s3 – state of unserviceability. These states are precisely defined for the ship main engine (ME). A hypothesis is proposed which explains the possibility of application...
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Adaptive Positioning Systems Based on Multiple Wireless Interfaces for Industrial IoT in Harsh Manufacturing Environments
PublikacjaAs the industrial sector is becoming ever more flexible in order to improve productivity, legacy interfaces for industrial applications must evolve to enhance efficiency and must adapt to achieve higher elasticity and reliability in harsh manufacturing environments. The localization of machines, sensors and workers inside the industrial premises is one of such interfaces used by many applications. Current localization-based systems...
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Factors affecting the conclusion of an arrangement in restructuring proceedings: evidence from Poland
PublikacjaThe EU Restructuring Directive (2019/1023) requires Member States to provide a preventive restructuring framework for financially distressed entities that remain viable or are likely to readily restore economic viability. The first step to a successful restructuring is the approval of an arrangement between the debtor and creditors. The main research objective of the article is to identify factors affecting the conclusion of an...
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FACTORS AFFECTING THE CONCLUSION OF AN ARRANGEMENT IN RESTRUCTURING PROCEEDINGS: EVIDENCE FROM POLAND
PublikacjaThe EU Restructuring Directive (2019/1023) requires Member States to provide a preventive restructuring framework for financially distressed entities that remain viable or are likely to readily restore economic viability. The first step to a successful restructuring is the approval of an arrangement between the debtor and creditors. The main research objective of the article is to identify factors affecting the conclusion of an...
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Chained machine learning model for predicting load capacity and ductility of steel fiber–reinforced concrete beams
PublikacjaOne of the main issues associated with steel fiber–reinforced concrete (SFRC) beams is the ability to anticipate their flexural response. With a comprehensive grid search, several stacked models (i.e., chained, parallel) consisting of various machine learning (ML) algorithms and artificial neural networks (ANNs) were developed to predict the flexural response of SFRC beams. The flexural performance of SFRC beams under bending was...
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Model Management for Low-Computational-Budget Simulation-Based Optimization of Antenna Structures Using Nature-Inspired Algorithms
PublikacjaThe primary objective of this study is investigation of the possibilities of accelerating nature-inspired optimization of antenna structures using multi-fidelity EM simulation models. The primary methodology developed to achieve acceleration is a model management scheme which the level of EM simulation fidelity using two criteria: the convergence status of the optimization algorithm, and relative quality of the individual designs...
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Experimental certification of more than one bit of quantum randomness in the two inputs and two outputs scenario
PublikacjaOne of the striking properties of quantum mechanics is the occurrence of the Bell-type non-locality. They are a fundamental feature of the theory that allows two parties that share an entangled quantum system to observe correlations stronger than possible in classical physics. In addition to their theoretical significance, non-local correlations have practical applications, such as device-independent randomness generation, providing...
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A Five-Leg Three-Level Dual-Output Inverter
PublikacjaClassical 3-level dual-output inverter, 3-L DOI, involves two similar 3-level inverters that provides a pair of 3-phase output voltages with same or different frequencies from common input voltage source. Flexibility of either operation of the constituting inverters is evident in this DOI; but total duplication of power switches is a major drawback. State of the art coupled 3-L DOIs reduce this drawback by providing series-shared...
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Training of Deep Learning Models Using Synthetic Datasets
PublikacjaIn order to solve increasingly complex problems, the complexity of Deep Neural Networks also needs to be constantly increased, and therefore training such networks requires more and more data. Unfortunately, obtaining such massive real world training data to optimize neural networks parameters is a challenging and time-consuming task. To solve this problem, we propose an easy-touse and general approach to training deep learning...
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Convolutional Neural Networks for C. Elegans Muscle Age Classification Using Only Self-Learned Features
PublikacjaNematodes Caenorhabditis elegans (C. elegans) have been used as model organisms in a wide variety of biological studies, especially those intended to obtain a better understanding of aging and age-associated diseases. This paper focuses on automating the analysis of C. elegans imagery to classify the muscle age of nematodes based on the known and well established IICBU dataset. Unlike many modern classification methods, the proposed...
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Highly Occupied Surface States at Deuterium-Grown Boron-Doped Diamond Interfaces for Efficient Photoelectrochemistry
PublikacjaPolycrystalline boron-doped diamond is a promising material for high-power aqueous electrochemical applications in bioanalytics, catalysis, and energy storage. The chemical vapor deposition (CVD) process of diamond forma-tion and doping is totally diversified by using high kinetic energies of deu-terium substituting habitually applied hydrogen. The high concentration of deuterium in plasma induces atomic arrangements and steric...
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A High-Arctic flow-through lake system hydrochemical changes: Revvatnet, southwestern Svalbard (years 2010–2018)
PublikacjaLake ecosystems are strongly coupled to features of their surrounding landscapes such as geomorphology, lithology, vegetation and hydrological characteristics. In the 2010–2018 summer seasons, we investigated an Arctic flow-through lake system Revvatnet, located in the vicinity of the coastal zone of Hornsund fjord in Svalbard, characterising its hydrological properties and the chemical composition of its waters. The lake system...
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Graph Representation Integrating Signals for Emotion Recognition and Analysis
PublikacjaData reusability is an important feature of current research, just in every field of science. Modern research in Affective Computing, often rely on datasets containing experiments-originated data such as biosignals, video clips, or images. Moreover, conducting experiments with a vast number of participants to build datasets for Affective Computing research is time-consuming and expensive. Therefore, it is extremely important to...
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Remote measurement of building usable floor area - Algorithms fusion
PublikacjaRapid changes that are taking place in the urban environment have significant impact on urban growth. Most cities and urban regions all over the world compete to increase resident and visitor satisfaction. The growing requirements and rapidity of introducing new technologies to all aspects of residents' lives force cities and urban regions to implement "smart cities" concepts in their activities. Real estate is one of the principal...
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Shrinking-shifting and amplifying-shifting device using transformation optics
PublikacjaBased on transformation optics (TO), this paper uses geometric divisions and linear coordinate transformations to design “shrinking-shifting—and reshaping”, and “amplifying-shifting—and reshaping” devices. The proposed devices can reshape the sizes and locations of the wrapped-objects inside the core-region. The shrinking-shifting device shrinks the larger object into a smaller one and shifts it to different location, whereas the...
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Pedestrian detection in low-resolution thermal images
PublikacjaOver one million people die in car accidents worldwide each year. A solution that will be able to reduce situations in which pedestrian safety is at risk has been sought for a long time. One of the techniques for detecting pedestrians on the road is the use of artificial intelligence in connection with thermal imaging. The purpose of this work was to design a system to assist the safety of people and car intelligence with the use...
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Mechanical behaviour of knit synthetic mesh used in hernia surgery
PublikacjaPurpose: There is a discussion in literature concerning mechanical properties and modelling of surgical meshes. An important feature of elastic modulus dependency on load history is taken into account in this paper, as implants are subjected to variable loading during human activity. The example of DynaMesh®-IPOM surgical implant is studied. Methods: The analysis is based on failure tension tests and cyclic loading and unloading...
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Prediction of energy consumption and evaluation of affecting factors in a full-scale WWTP using a machine learning approach
PublikacjaTreatment of municipal wastewater to meet the stringent effluent quality standards is an energy-intensive process and the main contributor to the costs of wastewater treatment plants (WWTPs). Analysis and prediction of energy consumption (EC) are essential in designing and operating sustainable energy-saving WWTPs. In this study, the effect of wastewater, hydraulic, and climate-based parameters on the daily consumption of EC by...
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A New Rehabilitation Device for Balance Impaired Individuals
PublikacjaIn the paper authors present a device designed to improve the rehabilitation process of people with balance impairment. The discussed device (JStep) utilizes a commercially available static standing frame (stander) modified in order to fit force sensing units under the feet and in the pillows around the hips of a patient. While executing rehabilitation tasks, the patient may compensate his balance deficiency by leaning on the pillows...
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Cast iron in the 19th-century building equipment
PublikacjaCast iron is a material, characteristics of which enable to receive extremely artistic elements. It maintains good strength properties at the same time. That combination of these seemingly contrary traits makes it a commodity that was widely used in the 19th century industry and architecture. These usages were not only as decorative elements, technical and structural ones. The production of new household utilities started, which...
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Using On-line Measurement by Electronic Nose and Computer Simulations for Real-time Control at WWTP
PublikacjaContinuous investigation of wastewater quality can be carried out by a device called an e-nose. One important feature of the proposed real-time control system for WWTP is that using on-line measurements by e-nose together (Figure 1) with technological sets picked on this basis by means of computer models, it is possible to change treatment process parameters, depending on the current quality of wastewater. It can be used for the...
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RDF dataset profiling - a survey of features, methods, vocabularies and applications
PublikacjaThe Web of Data, and in particular Linked Data, has seen tremendous growth over the past years. However, reuse and take-up of these rich data sources is often limited and focused on a few well-known and established RDF datasets. This can be partially attributed to the lack of reliable and up-to-date information about the characteristics of available datasets. While RDF datasets vary heavily with respect to the features related...
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Heterogeneous oxidation of highly boron-doped diamond electrodes and its influence on the surface distribution of electrochemical activity
PublikacjaThe electrochemical active surface area (EASA) of polycrystalline boron-doped diamond (BDD) electrodes is heterogeneous and can be affected by numerous factors. There is a strong need for proper consideration of BDD heterogeneity in order to improve this material's range of application in electrochemistry. Localized changes in surface termination due to the influence of oxidation agent result in increased surface resistance. The...
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A fast procedure of stress state evaluation in magnetically anisotropic steels with the help of a probe with adjustable magnetizing field direction
PublikacjaThe paper presents a novel approach to the stress state evaluation issue. It deals with a strongly (magnetically) anisotropic materials for which a direct interpretation of the Barkhausen effect (BE) intensity would lead to erroneous results. In such a case one has to take into account both the measured BE intensity and the orientation of the magnetisation direction relative to the magnetic easy axis. For the in plane stress distribution...
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A multi-analytical approach to the characterization of natural organic dyestuffs and inorganic substrates present in the 19th-century artistic oil paints manufactured by a French art materials supplier Richard Aines
PublikacjaThis paper presents a comprehensive analysis of artistic paints produced in the 19th century by a French art materials supplier Richard Ain`es. Improved mild extraction with hydrofluoric acid enabled the observation of intact organic dyes. Reversed-phase liquid chromatography with diode-array and mass spectrometry detection was utilised for the identification of 35 dyes present in yellow and red paint samples, and in selected plant...
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Long range molecular dynamics study of interactions of the eukaryotic glucosamine-6-phosphate synthase with fructose-6-phosphate and UDP-GlcNAc
PublikacjaGlucosamine-6-phosphate synthase (EC 2.6.1.16) is responsible for catalysis of the first and practically irreversible step in hexosamine metabolism. The final product of this pathway, uridine 5 diphospho Nacetyl- d-glucosamine (UDP-GlcNAc), is an essential substrate for assembly of bacterial and fungal cell walls. Moreover, the enzyme is involved in phenomenon of hexosamine induced insulin resistance in type II diabetes, which...
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Authenticity of Architectural Heritage in a Rebuilt City. Comments to Vaclav Havel’s Impression after His Visit in Gdansk in 2005
PublikacjaThere is no doubt that authenticity is a cardinal feature of architectural heritage and should be protected in the same way as historical and cultural value of monuments or their integrity. Sometimes, one decides to reconstruct a destroyed historical building due to important emotional, scientific, artistic or political reasons (e.g. the Zwinger Palace in Dresden or the Royal Castle in Warsaw). However, the solution which should...
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Expression of a GDSL esterase from Pseudomonas sp. S9 in Pichiapastoris
PublikacjaCold active lipolytic enzymes are promising to replace the conventional enzymes processes of biotechnological industries. One of the most important feature of the cold-active lipases and esterases is that they offer economic benefits through energy saving. In general, they exhibit high activity at low temperatures and low thermostability at moderate temperatures. Lipolytic enzyme EstS9 was isolated from Pseudomonas sp. S9. A multiple...
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Ontology of the Design Pattern Language for Smart Cities Systems
PublikacjaThe paper presents the definition of the design pattern language of Smart Cities in the form of an ontology. Since the implementation of a Smart City system is difficult, expensive and closely linked with the problems concerning a given city, the knowledge acquired during a single implementation is extremely valuable. The language we defined supports the management of such knowledge as it allows for the expression of a solution...
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DNA methylation in cancer development, diagnosis and therapy-multiple opportunities for genotoxic agents to act as methylome disruptors or remediators
PublikacjaThe role of DNA methylation and recently discovered hydroxymethylation in the function of the human epigenome is currently one of the hottest topics in the life sciences. Progress in this field of research has been further accelerated by the discovery that alterations in the methylome are not only associated with key functions of cells and organisms, such as development, differentiation and gene expression, but may underlie a number...
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Information retrieval with semantic memory model
PublikacjaPsycholinguistic theories of semantic memory form the basis of understanding of natural language concepts. These theories are used here as an inspiration for implementing a computational model of semantic memory in the form of semantic network. Combining this network with a vector-based object-relation-feature value representation of concepts that includes also weights for confidence and support, allows for recognition of concepts...
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Monte Carlo simulations of the fracture resistance degradation of asphalt concrete subjected to environmental factors
PublikacjaThe paper presents the results of laboratory tests of SCB (semi-circular beam) samples of asphalt concrete, subjected to the destructive effect of water and frost as well as the aging processes. The determined values of material parameters show significant dispersions, which makes the design of mixtures difficult. Statistical analysis of the test results supplemented by computer simulations made with the use of the proprietary...
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Rapid Design of 3D Reflectarray Antennas by Inverse Surrogate Modeling and Regularization
PublikacjaReflectarrays (RAs) exhibit important advantages over conventional antenna arrays, especially in terms of realizing pencil-beam patterns without the employment of the feeding networks. Unfortunately, microstrip RA implementations feature narrow bandwidths, and are severely affected by losses. A considerably improved performance can be achieved for RAs involving grounded dielectric layers, which are also easy to manufacture using...