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Escherichia coli K-12 suppressor-free mutants lacking early glycosyltransferases and late acyltransferases. Minimal lipopolysaccharide structure and induction of envelope stress response.
PublikacjaTo elucidate the minimal lipopolysaccharide (LPS) structure needed for the viability of Escherichia coli, suppressor-free strains lacking either the 3-deoxy-d-manno-oct-2-ulosonic acid transferase waaA gene or derivatives of the heptosyltransferase I waaC deletion with lack of one or all late acyltransferases (lpxL/M/P) and/or various outer membrane biogenesis factors were constructed. Δ(waaC lpxL lpxM lpxP) and waaA mutants exhibited...
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Selected aspects of two-phase flow studies using different visualization techniques in minichannels
PublikacjaThe subject of the study is to present the possibility of the use of visualization techniques in the researches on two-phase flows, carried out by the authors for many years. These works included as follows: heat transfer during the flow boiling, boiling crises and condensation in flow. The issue of the heat transfer intensification during the flow boiling through the channels of small diameters was analyzed. All this work focused...
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Thermohydraulic maldistribution reduction in mini heat exchangers
PublikacjaA detailed numerical investigation has been carried out to analyze the flow maldistribution in 50 parallel 1 mm × 1 mm rectangular minichannels and 1 mm depth minigap section with rectangular, trapezoidal, triangular or concave manifolds in Z-type flow configuration. The working medium was ethanol and the mass flow rate was 5 × 10−4 kg/s. Both sections were heated from the bottom side. Heat flux of 10 000 W/m2 and 5000 W/m2 was...
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Flow maldistribution and its mitigation in mini heat exchangers
PublikacjaIn the present paper, a detailed numerical investigation has been carried out to analyze the flow maldistribution in 50 parallel 1 mm x 1 mm rectangular minichannels and 1 mm depth minigap section with rectangular, trapezoidal, triangular or concave mani-folds in Z-type flow configuration (16 different cases). The working medium was ethanol and the mass flow rate was 5×10-4 kg/s. Both sections was heated from the bottom side. Heat...
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Preserving Trees in Automata
PublikacjaWe present a method to store additional information in a minimal automaton so that it is possible to compute a corresponding tree node number for a state. The number can then be used to retrieve additional information. The method works for minimal (and any other) deterministic acyclic finite state automata (DFAs). We also show how to compute the inverse mapping.
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Sailing Vessel Routing Considering Safety Zone and Penalty Time for Altering Course
PublikacjaIn this paper we introduce new model for simulation sea vessel routing. Besides a vessel types (polar diagram) and weather forecast, travel security and the number of maneuvers are considered. Based on these data both the minimal travelling costs and the minimal processing time are found for different vessels and different routes. To test our model the applications SailingAssistance wad improved. The obtained results shows that...
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On the hardness of computing span of subcubic graphs
PublikacjaIn the paper we study the problem of finding ξ-colorings with minimal span, i.e. the difference between the largest and the smallest color used.
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A VISION-BASED UNMANNED AERIAL VEHICLE NAVIGATION METHOD
PublikacjaThe satellite navigation systems are the main position sources for unmanned aerial vehicles (UAVs). This fact limits the area of UAVs operation to the places where radio signals is visible for a satellite navigation system receiver, mounted on the vehicle-outdoor navigation. Closed spaced are unavailable for vehicles which navigation is based on global satellite navigation systems (GNSS). Miniature UAV (MiniUAV) is able to operate...
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Computer-aided analysis of signals from a low-coherence Fabry-Perot interferometer used for measurements of biological samples
PublikacjaThe aim of the study was to develop an automated computer-aided system for analysis of spectrograms obtained from measurements of biological samples performed with a low-coherence Fabry-Pérot interferometer. Information necessary to determine dispersion characteristics of measured materials can be calculated from the positions of the maxima and minima that are present in their spectra. The main challenge faced during the development...
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Złote monety bulionowe – testowanie pasywnego charakteru inwestycji
PublikacjaCel – Wyznaczenie składu optymalnego portfela inwestycyjnego o minimalnym ryzyku, zawierającego inwestycję w polskie monety bulionowe (Orzeł Bielik) oraz inwestycję na polskiej giełdzie papierów wartościowych. Testowanie pasywnego charakteru inwestycji w monety. Metodologia badania – Konstrukcja portfeli inwestycyjnych o minimalnym ryzyku według teorii H.M. Markowitza. Autorska metoda badania pasywnego charakteru inwestycji...
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Analysis of the radiation dose in UV-disinfection flow reactors.
PublikacjaTheoretical considerations devoted to the variation of UV radiation doses in flow disinfection reactors are presented. The minimal time required by the fluid to pass the reactor was determined and combined with the radiation intensity. The active zones of the reactor were determined.
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Weak forms of shadowing in topological dynamics
PublikacjaWe consider continuous maps of compact metric spaces. It is proved that every pseudotrajectory with sufficiently small errors contains a subsequence of positive density that is point-wise close to a subsequence of an exact trajectory with the same indices. Also, we study homeomor- phisms such that any pseudotrajectory can be shadowed by a finite number of exact orbits. In terms of numerical methods this property (we call it multishadowing)...
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Graphs with equal domination and certified domination numbers
PublikacjaA setDof vertices of a graphG= (VG,EG) is a dominating set ofGif every vertexinVG−Dis adjacent to at least one vertex inD. The domination number (upper dominationnumber, respectively) ofG, denoted byγ(G) (Γ(G), respectively), is the cardinality ofa smallest (largest minimal, respectively) dominating set ofG. A subsetD⊆VGis calleda certified dominating set ofGifDis a dominating set ofGand every vertex inDhas eitherzero...
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Comparison of Two Nonlinear Predictive Control Algorithms for Dissolved Oxygen Tracking Problem at WWTP
PublikacjaThe wastewater treatment plant is classified as a complex system, due to its nonlinear dynamics, large uncertainty of disturbance inputs, multiple time scales in the internal process dynamics, and multivariable structure. The aeration process, in turn, is an important and expensive part of wastewater treatment plant operation. All operating parameters of the aeration in biological processes are to be precisely controlled to provide...
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A new library for construction of automata
PublikacjaWe present a new library of functions that construct minimal, acyclic, deterministic, finite-state automata in the same format as the author's fsa package, and also accepted by the author's fadd library of functions that use finite-state automata as dictionaries in natural language processing.
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Minimization of the number of periodic points for smooth self-maps of closed simply-connected 4-manifolds
PublikacjaLet M be a smooth closed simply-connected 4-dimensional manifold, f be a smooth self-map of M with fast grow of Lefschetz numbers and r be a product of different primes. The authors calculate the invariant equal to the minimal number of r-periodic points in the smooth homotopy class of f.
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Variable-temperature X-ray diffraction study of structural parameters of NH---S hydrogen bonds in triethylammonium and pyridinium silanethiolates
PublikacjaHydrogen bonds in triethylammonium and pyridinium silanethiolate salts have been characterized by X-ray, FT–IR and NMR spectroscopies. DFT calculations reveal strongly asymmetric double-well minima at the potential energy curves of the studied compounds
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Przejazdy kolejowe na modernizowanych i rewitalizowanych liniach kolejowych - przepisy a rzeczywistość
PublikacjaPrzy modernizacji i rewitalizacji dróg szynowych jednym z elementów infrastruktury, wymagającym szczególnej uwagi już na etapie planowania inwestycji, są przejazdy i przejścia w poziomie szyn. Obowiązujące przepisy krajowe pochodzą z lat 90. ubiegłego wieku i w wielu aspektach nie uwzględniają specyfiki najczęściej obecnie występujących na liniach kolejowych inwestycji modernizacyjnych i rewitalizacyjnych. W przypadku linii należących...
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Towards a classification of networks with asymmetric inputs
PublikacjaCoupled cell systems associated with a coupled cell network are determined by (smooth) vector fields that are consistent with the network structure. Here, we follow the formalisms of Stewart et al (2003 SIAM J. Appl. Dyn. Syst. 2, 609–646), Golubitsky et al (2005 SIAM J. Appl. Dyn. Syst. 4, 78–100) and Field (2004 Dyn. Syst. 19, 217–243). It is known that two non-isomorphic n-cell coupled networks can determine the same sets of...
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Hardware accelerated thin client for virtual reality applications
PublikacjaThin client applications often make minimal use of hardware available at the client side. This article focuses on the problem of creating portable thin client application for virtual reality applications, which allows use of client graphics hardware to aid rendering process. Some already available potential solutions are presented and entirely new protocol for client-server communication is presented to allow fast and reliable...
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Zero-Pole Space Mapping for CAD of Filters
PublikacjaIn this paper, we propose a new space-mapping technique tailored to the CAD of microwave filters. The goal of space mapping is to achieve a satisfactory design with the minimal number of fine model evaluations. In our approach, the filter is represented by a rational function. To quickly align the coarse and fine models, and to speed up the direct optimization of the coarse model, we propose matching the zeros and poles of a rational...
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Analysis of the interfacial energy of GaAs-Si hetrostructures
PublikacjaPrzeprowadzono obliczenia numeryczne energii powierzchni heterogranicy GaAs-Si z zastosowaniem półempirycznego modelu czteroparametrycznego Acklanda. Pokazano zależność energii heterogranicy od orientacji podłoża Si. Minima energetyczne otrzymano dla orientacji Si równych (011), (133), (112) i (113) co wskazuje ich zastosowanie do epitaksjalnego wzrostu warstw GaAs.
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Broadcast copies reveal the quantumness of correlations
PublikacjaWe study the quantumness of bipartite correlations by proposing a quantity that combines a measure of total correlations-mutual information-with the notion of broadcast copies-i.e., generally nonfactorized copies-of bipartite states. By analyzing how our quantity increases with the number of broadcast copies, we are able to classify classical, separable, and entangled states. This motivates the definition of the broadcast regularization...
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Development of beltless respiration and heartbeat sensor based on flexible piezoelectric film
PublikacjaThe main goal of this work is to report initial development on a respiration and heartbeat measurement sensor that can be used in continuous monitoring conditions at a minimal impact on the patient. General description of the sensor, consisting of a PVDF film and the bonding layer, is given. Open-circuit voltage generated by the sensor is estimated by analytical and numerical calculations. Analog and digital interface used to extract...
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Inwestycje w surowce w połączeniu z polskim rynkiem kapitałowym, analiza ryzyka i efektywności
PublikacjaW artykule przedstawiono główne kategorie i rodzaje inwestycji w surowce. Cel opracowania to zbadanie czy inwestycja w Warszawski Indeks Giełdowy stanowi główny składnik optymalnego tj. cechującego się minimalnym ryzykiem portfela inwestycyjnego. Ponadto ustalono czy wspomniana inwestycja tradycyjna stanowi najważniejszy element portfela inwestycyjnego odznaczającego się najwyższą efektywnością. Stwierdzono, że wszystkie z 29 analizowanych...
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Fatty acyl benzamido antibacterials based on inhibition of DnaK-catalyzed protein folding
PublikacjaWe have reported that the hsp70 chaperone DnaK from Escherichia coli might assist protein folding by catalyzing the cis/trans isomerization of secondary amide peptide bonds in unfolded or partially folded proteins. In this study a series of fatty acylated benzamido inhibitors of the cis/trans isomerase activity of DnaK was developed and tested for antibacterial effects in E. coli MC4100 cells. Nα-[Tetradecanoyl-(4-aminomethylbenzoyl)]-l-asparagine...
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The share of investments in gold and oil using the example of selected European stock exchanges– A comparative analysis
PublikacjaIn this article, optimal investment portfolios with minimal risk and maximum efficiency were calculated. The portfolios were designated for ten selected European stock exchanges, based on the listings of the twenty largest companies in each of those markets. All calculations were made based on company shares only, company shares and investments in gold, shares of companies and investments in crude oil as well as shares of companies...
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Entropy function application within the selection process of diagnostic parameters of marine diesel and gas turbine engines
PublikacjaThe paper presents the method of conducting an analysis of the diagnostic informativeness among the parameters characterizing the observed gas dynamic processes carried out within working spaces of marine diesel and gas turbine engines. An entropy function, as the measure of uncertainty of the identified states' set of the engine unfitness was applied. Having evaluated the diagnostic information the most adequate parameters were...
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Fatty acids as molecular carriers in cleavable antifungal conjugates
PublikacjaConjugates composed of C2-18 fatty acid (FA) residues as a molecular carrier and 5-fluorocytosine (5-FC) as an active agent, released upon the action of intracellular esterases on the ester bond between FA and “trimethyl lock” intramolecular linker, demonstrate good in vitro activity against human pathogenic yeasts of Candida spp. The minimal inhibitory concentrations (MIC) values for the most active conjugates containing caprylic...
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Molecular basis of essentiality of early critical steps in the lipopolysaccharide biogenesis in Escherichia coli K-12: requirement of MsbA, cardiolipin, LpxL, LpxM and GcvB
PublikacjaTo identify the physiological factors that limit the growth of Escherichia coli K-12 strains synthesizing minimal lipopolysaccharide (LPS), we describe the first construction of strains devoid of the entire waa locus and concomitantly lacking all three acyltransferases (LpxL/LpxM/LpxP), synthesizing minimal lipid IVA derivatives with a restricted ability to grow at around 21 °C. Suppressors restoring growth up to 37 °C of Δ(gmhD-waaA)...
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Biopsy needles coated with the antimicrobial coatings
PublikacjaDrug delivery systems are used to achieve higher therapeutic effects of medicaments in a specific diseased site with minimal toxicological effect. The used of biopolymers in drug delivery systems ensure the biocompatibility, biodegradability and low immunogenicity. Drug delivery systems enhance the drug delivery actively and can be used in different diseases. In this study, two types of polymer coatings were prepared and were...
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The power of silence? opinion contagion and the surprise of the polish 2005 parlamentary and presidential elections
PublikacjaThis paper investigates opinion contagion in collective behaviour using threshold model (Granovetter (1978), Krassa(1988)). The theoretical background is the spiral of silence concept developed by Noelle-Neumann (1974), arguing that people only assert their opinions if they perceive a minimal support from a relevant proportion of others. We apply the model to explain the dispersion between pre-elections preferences and the final...
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Simple sufficient condition for subspace to be completely or genuinely entangled
PublikacjaWe introduce a simple sufficient criterion, which allows one to tell whether a subspace of a bipartite or multipartite Hilbert space is entangled. The main ingredient of our criterion is a bound on the minimal entanglement of a subspace in terms of entanglement of vectors spanning that subspace expressed for geometrical measures of entanglement. The criterion is applicable to both completely and genuinely entangled subspaces. We...
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Stability of roof trusses stiffened by trapezoidal sheeting and purlins
PublikacjaIn the article linear buckling analysis of a set of steel trusses braced by purlins and trapezoidal sheeting are conducted. The buckling load factor due to the height of a corrugated sheeting profile is investigated in parametric studies. The minimal height of trapezoidal sheeting required for preventing the sheeting and chords of the trusses against the buckling is obtained. Two groups of models are considered: “axial” model as...
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Minimization of the number of periodic points for smooth self-maps of simply-connected manifolds with periodic sequence of Lefschetz numbers
PublikacjaLet f be a smooth self-map of m-dimensional, m ≥ 4, smooth closed connected and simply-connected manifold, r a fixed natural number. For the class of maps with periodic sequence of Lefschetz numbers of iterations the authors introduced in [Graff G., Kaczkowska A., Reducing the number of periodic points in smooth homotopy class of self-maps of simply-connected manifolds with periodic sequence of Lefschetz numbers, Ann. Polon. Math....
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Comparison of natural frequencies of a circular saw blade obtained empirically and with FEM
PublikacjaThe knowledge of the natural frequencies’ values of circular saw blades is necessary to determine the minimal critical rotational speed in which they can work with required stability. Moreover, testing the circular saw blades with more complicated shapes, e.g. which have additional holes inside blades for cleaning knifes or additional indirect teeth in gullets, reveals some kind of problematic properties of these saw blades. The...
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Edible Lepidoptera as human foods – a comprehensive review
PublikacjaAs the global population continues to grow, traditional protein sources like meat and fish are becoming increasingly unsustainable due to their environmental impact. Edible insects, on the other hand, are highly nutritious, require minimal resources to produce, and emit significantly fewer greenhouse gases than traditional livestock. Lepidoptera, one of the most diverse insect orders, contains some popular edible species that have...
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Phenotypic consequences of the LYS4 gene disruption in Candida albicans
PublikacjaThe main scientific purpose of our studies was to verify the hypothesis that homoaconitase (HA) from Candida albicans, an enzyme catalyzing a second step of the α-aminoadipate pathway (AAP) of L-Lys biosynthesis may become a new target for antifungal chemotherapy. Previous studies indicated that the A. fumigatus mutant lacking the functional lysF gene, encoding HA, exhibited attenuated virulence in a low-dose mouse infection model...
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Optymalizacja długości krzywych przejściowych przy budowie i modernizacji linii kolejowych
PublikacjaW drogach szynowych przy obliczaniu długości krzywych przejściowych analizowane są jedynie warunki na ich długość minimalną. Odmienna sytuacja występuje przy projektowaniu dróg, gdzie rozpatrywane są też warunki na długość maksymalną. Spośród nich szczególną rangę ma warunek poprawności geometrycznej układu. W artykule zawarto jego adaptację do specyfiki trasy kolejowej. Sformułowano cel optymalizacji przy obliczaniu długości krzywej...
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Experimental certification of an informationally complete quantum measurement in a device-independent protocol
PublikacjaMinimal informationally complete positive operator-valued measures (MIC-POVMs) are special kinds of measurement in quantum theory in which the statistics of their d2-outcomes are enough to reconstruct any d-dimensional quantum state. For this reason, MIC-POVMs are referred to as standard measurements for quantum information.Here, we report an experiment with entangled photon pairs that certifies, for what we believe is the first...
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Efficient synthesis and antifungal investigation of nucleosides’ quaternary ammonium salt derivatives
PublikacjaQuaternary ammonium salts are a group of compounds with diverse biological properties, the most important of which are their antiviral, antibacterial, and antifungal activities. The quaternization reactions of 5'-O-tosyl derivatives of uridine and thymidine with triethylamine, trimethylamine, 4-(N ,N -dimethylamino)pyridine, 2-methylpyridine, and pyridine are described in this article. Two of the synthesized compounds are exceptional...
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Scheduling jobs to contain a natural disaster: a model and complexity
Publikacjathis paper is devoted to the problem of scheduling suppression units so that a natural disaster is dealt with as efficient as possible. the concept of deteriorating jobs is adopted, that is, the formal model of scheduling represents linearly increasing value loss as the disaster remains unsuppressed and increasing time for its suppression. more precisely, two different goals are considered: finding a suppression schedule of minimal...
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Evolution of Animats Following a Moving Target in an Artificial Ecosystem
PublikacjaMany biological animals, even microscopically small, are able to track moving sources of food. In this paper, we investigate the emergence of such behavior in artificial animals (animats) in a 2-dimensional simulated liquid environment. These "predators" are controlled by evolving artificial gene regulatory networks encoded in linear genomes. The fate of the predators is determined only by their ability to gather food and reproduce—no...
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Design, Realization and Measurements of Enhanced Performance 2.4 GHz ESPAR Antenna for Localization in Wireless Sensor Networks
PublikacjaThis paper presents the design, realization and measurements of an Electronically Steerable Parasitic Array Radiator (ESPAR) antenna with enhanced performance of estimating the incoming signal direction. Designed antenna is dedicated for 2.4 GHz ISM applications with emphasis on Wireless Sensor Networks (WSN). Proposed antenna provides different radiation patterns by proper configuration of the parasitic elements. Thus, several...
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Transport deficiency is the molecular basis of Candida albicans resistance to antifungal oligopeptides
Publikacja(FMDP), an inhibitor of glucosamine-6-phosphate synthase, exhibited growth inhibitory activity against Candida albicans, with minimal inhibitory concentration values in the 0.05–50 mg/L range. Uptake by the peptide permeases was found to be the main factor limiting an anticandidal activity of these compounds. Di- and tripeptide containing FMDP (F2 and F3) were transported by Ptr2p/Ptr22p peptide transporters (PTR) and FMDP-containing...
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Antena mikropaskowa o poszerzonym paśmie pracy
PublikacjaW pracy przedstawiono wyniki badań dotyczące anteny mikropaskowej o poszerzonym paśmie pracy. Poprzez zastosowanie układu dwóch szczelin sprzęgających we wspólnej warstwie masy uzyskano 29% pasmo pracy (0.96GHz -1.25 GHz) mierzone przy poziomie WFS < 1.5. Ze względu na obserwowane w charakterystyce promieniowania minima, wykonano drugą wersję anteny ze zmodyfikowanymi wymiarami warstwy masy. Efektemtego zabiegu było uzyskanie anteny...
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Potential energy surfaces of the low-lying electronic states of the Li+LiCs system
PublikacjaAb initio quantum chemistry calculations are performed for the mixed alkali triatomic system. Global minima of the ground and first excited doublet states of the trimer are found and Born-Oppenheimer potential energy surfaces of the Li atom interacting with the LiCs molecule were calculated for these states. The lithium atom is placed at various distances and bond angles from the lithium-caesium dimer. Three-body nonadditive forces...
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Computations of the least number of periodic points of smooth boundary-preserving self-maps of simply-connected manifolds
PublikacjaLet $r$ be an odd natural number, $M$ a compact simply-connected smooth manifold, $\dim M\geq 4$, such that its boundary $\partial M$ is also simply-connected. We consider $f$, a $C^1$ self-maps of $M$, preserving $\partial M$. In [G. Graff and J. Jezierski, Geom. Dedicata 187 (2017), 241-258] the smooth Nielsen type periodic number $D_r(f;M,\partial M)$ was defined and proved to be equal to the minimal number of $r$-periodic points...
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Examining Impact of Speed Recommendation Algorithm Operating in Autonomous Road Signs on Minimum Distance between Vehicles
PublikacjaAn approach to a new kind of recommendation system design that suggests safe speed on the road is presented. Real data obtained on roads were used for the simulations. As part of a project related to autonomous road sign development, a number of measurements were carried out on both local roads and expressways. A speed recommendation model was created based on gathered traffic data employing the traffic simulator. Depending on...
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E-textbook technologies for academics in medical education
PublikacjaPublic universities in Poland receive fairly limited financial support for creating e-textbooks and lack the appropriate ICT competences among teaching staff, especially in the case of non-technical universities. The authors propose a pedagogical and technological paradigm for e-textbooks in medical education using open source software with minimal IT skills required. Technologies used to develop e-textbooks are connected with:...