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Dynamic Headspace Sampling as an Initial Step for Sample Preparation in Chromatographic Analysis
PublicationThis work represents a brief summary of the use of dynamic headspace (DHS) as a technique for sample preparation in chromatographic analysis. Despite numerous developments in the area of analyte isolation and enrichment, DHS remains one of the fundamental methods used with GC. In our opinion, interest in this technique will not diminish significantly because it conforms to stipulations of green analytical chemistry. Moreover, DHS...
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Efficient three-dimensional fluorescence measurements for characterization of binding properties in some plants
PublicationThe main aim of this research was to characterize some plants and to determine their similarities and differences, using spectroscopic methods. The interactions of soluble polyphenols of different plants with human serum albumin (HSA) were investigated by 3D-fluorescence. The obtained fluorescence results allow to classify the investigated plants according to their binding properties. The HSA-binding capacities of these plants...
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The importance and availability of marine certified reference materials
PublicationTraceability and reliable results are the two pillars of analytical methods; certified reference materials (CRMs) meet this requirement. ISO 17034:2016 credentials provide brief information on general requirements for the competence of Reference Material Producers (RMPs). The different types of CRMs have been produced in recent years for chemical analysis in food, water, soil, and sediment matrices in recent years. This review...
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Electronic Noses for Indoor Air Quality Assessment
PublicationThis chapter presents a proposal of the use of electronic noses in the monitoring of indoor air quality. The main focus is put on the detailed characteristics of today’s indoor air quality control methods, the types of pollution in the air, and the development of electronic noses for air testing. Currently, scientists seek methodological and structural solutions that would enable real-time online indoor air control. It has been...
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Polysaccharides-Based Hybrid Materials for Bio- and Non-Bio Sectors. Edytorial
PublicationThis special edition spotlights the recent research in the design, development, and emerging applications of polysaccharides-based hybrid materials for biotechnological and biomedical purposes. All the articles published in this issue underscore the significance of materials derived from cellulose, alginate, chitosan, starch, and carrageenan for various applications, including enzyme production, encapsulation, targeted drug delivery,...
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Combination of homogeneous liquid–liquid extraction and vortex assisted dispersive liquid–liquid microextraction for the extraction and analysis of ochratoxin A in dried fruit samples: Central composite design optimization
PublicationThis paper presents a new analytical procedure based on combination of homogeneous liquid–liquid extraction (HLLE) and vortex-assisted dispersive liquid–liquid microextraction (VA-DLLME) for the accurate and reliable determination of ochratoxin A (OTA) in dried fruit samples. To enable selective extraction of the OTA, six hydrophobic deep eutectic solvents (hDESs) were prepared and tested as extraction solvents. Optimization of...
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Non-carcinogenic human risk assessment of the consumption of infant formulas contaminated with total and organic mercury
PublicationThe quality of food is paramount for the healthy growth and development of children, with nutrients and contaminants like mercury playing significant roles here. In this study, the levels of total mercury (THg) and methylmercury (MeHg) in infant formulas such as the first infant formula, hypoallergenic formula, comfort formula against colic and constipation, and anti-reflux formula were investigated. A validated and optimized cold...
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Analysis of bisphenols and their derivatives in infant and toddler ready-to-feed milk and powdered milk by LC MS/MS
PublicationReady-to-feed milk and powdered milk packed in different types of packaging lined with resin are chosen as the primary or complementary diet for infants and toddlers. An HPLC–MS/MS method was established and validated for the determination of nineteen bisphenols and their derivatives in such samples. The recoveries were from 31% to 120%, while the values of limit of detection and quantification were in the range of 0.24–0.40 and...
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Antioxidant activities and bioactive components in some berries
PublicationThe objective of this study was to evaluate the antioxidant and binding effects of gooseberry, a less-stud-ied berry, and to compare with blueberry and cranberry in the model of interaction with human serum albumin (HSA). The relationship between the scavenging properties of dietary polyphenols of the selected berries and their affinities for HSA were investigated by fluorescence anal- ysis. In order to perform the extraction and...
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Capillary Electrophoresis in Determination of Low Molecular Mass Organic Acids
PublicationThis paper presents overviews the capabilities of capillary electrophoretic techniques to determine low molecular mass organic acids in a variety of aqueous, gas and solid samples. It mainly focuses on short - chain carboxylic acids containing one or more carboxylic groups and possibly some other functional groups (hydroxyl, keto-, amino- etc.). Finally the procedures applied to the determine the acids in different matrices are...
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Zastosowanie założeń koncepcji efektuacji w organizowaniu wydarzenia kulturalnego – studium przypadku
PublicationThe paper presents how the effectuation model, which stems from the business studies, can be applied as a tool for analysing the activities undertaken as part of culture organisation. The key element of this model involves the control-based approach to the future instead of making predictions about the future. The outline of the theoretical background is followed by the results of the qualitative research, where the answers were...
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Polymeric porous membranes as solid support and protective material in microextraction processes: A review
PublicationPolymeric porous membranes have proven to be a versatile and efficient material for microextraction processes. In analytical practice, they are used both as a solid support and as a protective medium for improved sample preparation in various analytical applications. Polymeric porous membranes offer unique advantages in microextraction due to their large surface area and controllable pore size, eliminating interfering effects from...
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Mercury concentrations in Antarctic zooplankton with a focus on the krill species, Euphausia superba
PublicationThe Antarctic is the most isolated region in the world; nevertheless, it has not avoided the negative impact of human activity, including the inflow of toxic mercury (Hg). Hg deposited in the Antarctic marine environment can be bioavailable and accumulate in the food web, reaching elevated concentrations in high-trophic-level biota, especially if methylated. Zooplankton, together with
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Diversity and distribution of Tardigrada in Arctic cryoconite holes
PublicationDespite the fact that glaciers and ice sheets have been monitored for more than a century, knowledge on the glacial biota remains poor. Cryoconite holes are water-filled reservoirs on a glacier’s surface and one of the most extreme ecosystems for microinvertebrates. Tardigrada, also known as water bears, are a common inhabitant of cryoconite holes. In this paper we present novel data on the morphology, diversity, distribution and...
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Toward the next generation of air quality monitoring: Mercury
PublicationMercury is a global pollutant that is ubiquitous in the environment. Enrichment of mercury in the biosphere as the result of human activities and subsequent production of methylmercury (MeHg) has resulted in elevated concentrations in fish, wildlife and marine mammals globally. Elemental mercury (Hg0) is the most common form of mercury in the atmosphere, and the form that is most readily transported long distances from its emission...
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Beta-Galactosidases of Pseudoalteromonas sp. – Characteristics and application for lactose hydrolysis and galactopyranosides synthesis
PublicationBeta-galactosidase [EC 3.2.1.23] is an enzyme that catalyzes the hydrolysis of O-glycosidic linkages in galactosides. It has biotechnological applications in the dairy industry for the production of milk with a low content of lactose and galactooligosacharides for use in healthy food. The transgalactosylation activity of beta-galactosidase can be used in the synthesis of alkyl galactopyranosides. Due to the fact that conditions of...
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The properties, functions and use of selenium compounds in living organisms
PublicationSelenium occurs in the environment in inorganic and organic compounds. For many years it was regarded as a toxic element, causing numerous illnesses and diseases. But research in the last 50 years has revealed a "bright side" to this element, especially as a component of selenoproteins, selenium makes a significant contribution to the health of humans and animals. The selenium content in an organism depends on its concentration...
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Modern solutions in the field of microextraction using liquid as a medium of extraction
PublicationThe monitoring of compounds present in samples at trace/ultra-trace level usually requires a preliminary step of isolation and/or enrichment of analytes. Against, sample preparation is considered as crucial part of whole analytical procedures, in particular in samples characterized by complex matrices composition. Several new miniaturized extraction techniques is introduced and extensively applied to different type of samples....
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Modern trends in solid phase extraction: New sorbent media
PublicationBased on the recently published literature, this review provides an update of the most important features and application of formats and devices employed in solid phase extraction (SPE). Special attention was paid on new trapping media proposed in SPE prior the chromatography analysis, based on the use of nanostructured materials, including carbon nanomaterials, electrospun nanofibers, dendrimes and magnetic nanoparticles, molecular...
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COMPOSITION OF BIOACTIVE SECONDARY METABOLITES AND MUTAGENICITY OF SAMBUCUS NIGRA L. FRUIT AT DIFFERENT STAGES OF RIPENESS
PublicationThe relationship between the content of bioactive compounds and mutagenic activity of elderberry fruit at different stages of ripeness was investigated. Signifi cant differences in the antioxidant profi les (TLC, HPLC with post-column derivatization) and antioxidant activity (ABTS, DPPH, and FC tests) were observed for studied elderberry extracts. The more ripen the fruit at the time of harvest were, the higher the content of...
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Cloning, expression, purification and characterization of recombinant trehalose synthase from Deinococcus radiodurans.
PublicationTrehalose (α-D-glucopyranosyl-1,1-α-D-glucopyranoside) is a nonreducing disacharide in which the two glucose molecules are linked trough a α-1,1-glycosidic bond. Trehalose is readily hydrolyzed to glucose and can be used as a reserve of that sugar in the cell. The presence of trehalose was found in the cells of fungi and yeasts, bacteria, nematodes, insects, eggs, pupae and some plants. The characteristics of trehalose make it...
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Latest Insights on Novel Deep Eutectic Solvents (DES) for Sustainable Extraction of Phenolic Compounds from Natural Sources
PublicationPhenolic compounds have long been of great importance in the pharmaceutical, food, and cosmetic industries. Unfortunately, conventional extraction procedures have a high cost and are time consuming, and the solvents used can represent a safety risk for operators, consumers, and the environment. Deep eutectic solvents (DESs) are green alternatives for extraction processes, given their low or non-toxicity, biodegradability, and reusability....
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Activity and primary characterization of enzyme from Thermus ruber cells catalyzing conversion of maltose into trehalose
PublicationTermofilna bakteria Thermus ruber wytwarza syntazę trehalozy użyteczną do jednoetapowego przekształcania maltozy w trehalozę. Enzym ten wykazuje największą aktywność w temperaturze 65 C przy pH 6,5. Największą wydajność wytwarzania enzymu osiągano podczas hodowli drobnoustroju w temp. 55 stC w podłożu zawierającym 0,5% peptonu,0,1% ekstraktu drożdżowego oraz 0,% maltozy lub skrobi.
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The new method of settings the small fishes' backs in the desired direction
PublicationW artykule przeanalizowano znane sposoby ukierunkowywania małych ryb grzbietem w jedną stronę. Podano założenia nowego sposobu orientowania poprzecznego. Przedstawiono wstępną analizę teoretyczną orientowania poprzecznego opartego na wykorzystaniu pary równoległych, nachylonych do poziomu, współbieżnych, gładkich rolek. Przeprowadzono weryfikację doświadczalną nowego sposobu na przykładzie szprotów bałtyckich. Ze względu na wstępny...
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The bioactivity of processed garlic (Allium sativum L.) as shown in vitro and in vivo studies on rats
PublicationCzosnek w trakcie procesu obróbki traci znaczną część swojej bioaktywności. Właściwości przeciwutleniające zmierzone metodą FRAP i DPPH stwierdzono na najwyższym poziomie w przypadku czosnku surowego oraz po krótkim okresie gotowania. Badania na szczurach poddanych diecie z dodatkiem krótkogotowanego czosnku potwierdziły, że proces ten nie zaburza właściwości przeciwutleniających surowego czosnku. U zwierząt stwierdzono poprawę...
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Methodological evaluation of method for dietary heavy metal intake
PublicationNarażenie człowieka na działanie zanieczyszczeń środowiskowych jest poważnym problemem w obecnych czasach. Metale ciężkie są uważane za toksyczne dla człowieka ze względu na tendencję kumulowania się w wybranych tkankach organizmu. Ponadto, ich obecność w ustroju jest przyczyną wielu schorzeń. Substancje szkodliwe mogą przenikać do organizmu kilkoma drogami, m.in. poprzez spożywanie skażonej żywności. Biorąc pod uwagę ryzyko związane...
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Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage
PublicationExposure to high levels of different environmental pollutants is known to be associated with induction of DNA damage in humans. Thus DNA repair is of great importance in preventing mutations and contributes crucially to the prevention of cancer. In our study we have focused on quantitative analysis of Gentiana asclepiadea aqueous or methanolic extracts obtained from flower and haulm, their antioxidant potency in ABTS post-column...
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Antioxidant properties and bioactive constituents of some rare exotic thai fruits and comparison with conventional fruits. in vitro and in vivo studies
PublicationPrzedmiotem badań były ekstrakty uzyskane zarówno z egzotycznych owoców z terenu Tajlandii (durian, snake fruit, mangosteen) jak i owoców ''konwencjonalnych'' (mango, kiwi, awokado) w celu porównania zawartości związków bioaktywnych i aktywności przeciwutleniającej.Wyniki badań prowadzonych zarówno in vitro jak i in vivo mogą być wykorzystane przy opracowywaniu diety. W trakcie badań in vitro wykorzystano testy do określania potencjału...
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Beta-galactosidase activity of Meiothermus ruber cells
PublicationFreeze-dried cells of Meiothermus ruber catalyses cleavage of o-nitrophenyl-b-D-galactopiranoside (oNPb-gal) and conversion of lactose into glucose and galactose. The permeabilization with 2%toluene,20%ethanol and 20%acetone increased enzymatic activity from 74.87 U/g of lyophilized cells up to 129.44,114.38 and 90.19 U/g,respectively. Ethanol was an effective permeabilizing agent and its efficiency was dependent on the concentration,...
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Immobilization on magnetic nanoparticles of therecombinant trehalose synthase from Deinococcusgeothermalis
PublicationtIn our study the gene encoding trehalose synthase from Deinococcus geothermalis was cloned and overexpressed inEscherichia coli Rosetta (DE3)pLysS. Wild-type trehalose synthase has been purified from host protein after cell dis-ruption and precipitation at 20% ammonium sulphate saturation. Recombinant trehalose synthase was immobilizedonto glutaraldehyde activated silanized magnetic ferrous-ferric oxide by using covalent binding...
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Composition of the Essential Oil of Bidens tripartita L. Roots and Its Antibacterial and Antifungal Activities
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Effect of substrate type on the field performance and chemical composition of highbush blueberry cv.Patriot
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Hepatoprotective effect of quercetin: From chemistry to medicine
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Oxidative stability assessment of industrial and laboratory-pressed fresh raspberry seed oil (Rubus idaeus L.) by differential scanning calorimetry
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The combined effect of moderate pressure and chitosan on Escherichia coli and Staphylococcus aureus cells suspended in a buffer and on natural microflora of apple juice and minced pork
PublicationW pracy określono przeżywalność bakterii Escherichia coli i Staphylococcus aureus oraz naturalnej mikroflory soku jabłkowego i rozdrobnionego mięsa świńskiego po działaniu ciśnienia 193 MPa w temp. -20 C w obecności chitozanu. Stwierdzono, że traktowanie mikroorganizmów ciśnieniem w obecności chitozanu pozwala na przedłużenie trwałości badanych produktów przechowywanych w temperaturze chłodniczej o około 8-10 dni.
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Application of Electronic Nose Based on Fast GC for Authenticity Assessment of Polish Homemade Liqueurs Called Nalewka
PublicationThe possibility of application of electronic nose based on fast GC for differentiation of three types of cherry liqueurs, namely Polish homemade liqueurs called nalewka, commercially available liqueurs labeled as nalewka, and commercial liqueurs, was investigated. This device was equipped with two parallel connected columns with different stationary phases coupled to two ultrasensitive flame ionization detectors (μ-FIDs). Four...
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Effect of long-term cold storage on physicochemical attributes and bioactive components of kiwi fruit cultivars
PublicationVarious kiwi fruit cultivars, bred in Korea, were kept in cold storage for 8–24 weeks for possible increase of their quality. Firmness significantly decreased at initial time in all cultivars.The rate of softeningwas the slowest in “Hayward”, followed by “Hort16A”, “Haenam”, “Daheung”, “Bidan”, “Hwamei”, and “SKK 12”. Sensory value increased with decreasing of firmness. Soluble solids content increased with storage time while acidity gradually...
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The Analysis of Vodka: A Review Paper
PublicationVodka is the most popular alcoholic beverage in Poland, Russia and other Eastern European countries, made from ethyl alcohol of agricultural origin that has been produced via fermentation of potatoes, grains or other agricultural products. Despite distillation and multiple filtering, it is not possible to produce 100 % ethanol. The solution with a minimum ethanol content of 96 %, which is used to produce vodkas, also contains trace amounts...
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Simultaneous Determination of Indolic Compounds in Plant Extracts by Solid-Phase Extraction and High-Performance Liquid Chromatography with UV and Fluorescence Detection
PublicationA high-performance liquid chromatographic method with UV and fluorescence detection (HPLC-DAD-FLD) was developed for simultaneous determination of indolic compounds in plant material. Indole-3-carbinol (I3C), indole-3-acetic acid (I3AA), indole-3-acetonitrile (I3ACN), and 3,3′-diindolylmethane (DIM) were used as representative compounds that cover a wide spectrum of indole structures occurring in nature. For concentration and purification...
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In vitro antioxidative and binding properties of phenolics in traditional, citrus and exotic fruits
PublicationMany polyphenols bind proteins, therefore our research was focused on the potential of protein binding to polyphenols of investigated fruits and their health-related effects. The contents of polyphenols and related antioxidant activities of traditional, citrus and exotic fruits were compared. The presence of polyphenols (flavonoids and phenolic acids) in the investigated samples and their interaction with human serum albumin (HSA)...
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Differentiation Between Spirits According to Their Botanical Origin
PublicationAgricultural distillates are used for the production of spirit beverages. As more and more products with a specific botanical composition specified on the label are produced (e.g. rye vodkas, wheat vodkas and Polish Vodka, which cannot be produced with the addition of maize distillate), it is necessary to look for quicker and more accurate methods allowing the determination of botanical origin of alcoholic beverages and materials...
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The extended version of restriction analysis approach for the examination of the ability of low-molecular-weight compounds to modify DNA in a cell-free system
PublicationOne of the primary requirements in toxicology is the assessment of ability of chemicals to induce DNA covalent modification. There are several well-established methods used for this purpose such as 32P-Postlabeling or HPLC-MS. However, all of these approaches have difficult to overcome limitations, which prevents their use in genotoxin screening. Here, we describe the simple protocol exploiting specificity of restriction enzymes...
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Chemical Quality of Bottled Waters: A Review
PublicationBottled water has become very popular for quenching thirst and as a dietary (mineral) supplement. Theplethora of natural mineral waters precludes any unequivocal system of classification, which makes it difficult for theconsumer to choose a water with properties that suits him/her exactly. The ever-increasing popularity of bottled watersmeans that it is of the utmost importance to determine not only their mineral content, but above...
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The changes of antioxidant defense system caused by quercetin administration do not lead to DNA damage and apoptosis in the spleen and bone marrow cells of rats
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In‐milk inactivation of Escherichia coli O157:H7 by the environmental lytic bacteriophage ECPS‐6
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Chocolate desserts with ricotta hydrolysates: In vitro study of inhibitory activity against angiotensin‐converting enzyme and cholinesterase
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Phytocomponents and evaluation of acetylcholinesterase inhibition by Ginkgo biloba L. leaves extract depending on vegetation period
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Effect of sweet grass extract against oxidative stress in rat liver and serum
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Antioxidant properties of black tea in alcohol intoxication
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Exposure to cadmium, lead and mercury in the adult population from Eastern Poland, 1990–2002
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