Search results for: TAGS
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Properties of recombinant trehalose synthase from Deinococcus radiodurans expressed in Escherichia coli
PublicationA trehalose synthase gene from Deinococcus radiodurans(DSMZ 20539)containing 1659 bp reading frame encoding 552 amino acids was amplified using PCR. The gene was finally ligated into pET30Ek/LIC vector and expressed after isopropyl β-d-thiogalactopyranoside induction in Escherichia coli (DE3) Rosetta pLysS. The recombinanttrehalose synthase (DraTreS) containing a His6-tag at the C-terminus was purified by metal affinity chromatography...
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Unconventional and user-friendly sampling techniques of semi-volatile organic compounds present in an indoor environment: An approach to human exposure assessment
PublicationThe commonly applied solutions used to assess the potential risk of human exposure to semi-volatile organic compounds (SVOCs) are based on the investigation of biological samples collected in an invasive or non-invasive manner. For SVOCs, which are typically introduced to humans through the respiratory system, dermal adsorption, or digestive system, sampling solutions generally used in the indoor environments are classified as...
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Determination of Odour Interactions of Three-Component Gas Mixtures Using an Electronic Nose
PublicationThe paper presents an application of an electronic nose prototype comprised of six TGS-type sensors and one PID-type sensor to identify odour interaction phenomena in odorous three-component mixtures. The investigation encompassed eight odorous mixtures—toluene-acetone-triethylamine and formaldehyde-butyric acid-pinene — characterized by different odour intensity and hedonic tone. A principal component regression (PCR) calibration...
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Technological aspects of manufacturing polymer packaging materials degradable in seawater environment
PublicationIn recent years, the number of polymer materials used in the food packaging market was growing extremely fast. This contributed to depletion of non-renewable resources and more intense degradation of the environment due to resistant polymer. Therefore, such a dramatic situation has forced researchers to look for biodegradable materials, decomposable under action of microorganism. Currently, only a small number of researches examine...
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The complexity of minimum-length path decompositions
PublicationWe consider a bi-criteria generalization of the pathwidth problem, where, for given integers k, l and a graph G, we ask whether there exists a path decomposition P of G such that the width of P is at most k and the number of bags in P, i.e., the length of P, is at most l. We provide a complete complexity classification of the problem in terms of k and l for general graphs. Contrary to the original pathwidth problem, which is fixed-parameter...
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Structural and spectroscopic investigation of new luminescent hybrid materials based on calix[4]arene-tetracarboxylate and Ln 3+ ions (Ln = Gd, Tb or Eu)
PublicationLanthanide-calixarene hybrid materials are of particular interest due to the combination of the interesting properties of the ligand cavity-like structure and the luminescent features of lanthanides. The aim of this study was to synthesize and investigate the photophysical properties of Eu3+, Tb3+ and Gd3+ hybrids based on calix[4]arene-tetracarboxylate. The preparation of two structurally different...
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Cross-linking of sodium caseinate-structured emulsion with transglutaminase alters postprandial metabolic and appetite responses in healthy young individuals
PublicationThe physico-chemical and interfacial properties of fat emulsions influence lipid digestion and may affect postprandial responses. The aim of the present study was to determine the effects of the modification of the interfacial layer of a fat emulsion by cross-linking on postprandial metabolic and appetite responses. A total of fifteen healthy individuals (26·5 (sem 6·9) years and BMI 21·9 (sem 2·0) kg/m2) participated in a cross-over...
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Solid-State Modification of Poly(butylene terephthalate) with a Bio-Based Fatty Acid Dimer Diol Furnishing Copolyesters with Unique Morphologies
PublicationCopolyesters based on poly(butylene terephthalate) (PBT) and a bio-based fatty acid dimer diol (FADD) were prepared by solid-state modification (SSM). The chemical incorporation of the FADD into the backbone of the PBT was proven using various techniques. It was clear that the incorporation rate was diffusion-limited rather than reaction-limited. From quantitative 13C NMR a nonrandom chemical microstructure was obtained at all...
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Synthesis and Structure of Novel Copper(II) Complexes with N,O- or N,N-Donors as Radical Scavengers and a Functional Model of the Active Sites in Metalloenzymes
PublicationToevaluatetheantioxidantactivityofpotentialsyntheticenzymemimetics,wepreparednewfivecopper(II)complexesviaaself‐assemblymethodandnamedthem[Cu(2‐(HOCH2)py)3](ClO4)2(1),[Cu(2‐(HOCH2)py)2(H2O)2]SiF6(2),[Cu2(2‐(HOCH2CH2)py)2(2‐(OCH2CH2)py)2](ClO4)2(3),[Cu(pyBIm)3](BF4)2∙1.5H2O(4)and[Cu(py2C(OH)2)2](ClO4)2(5).ThesyntheticprotocolinvolvedN,O‐ orN,N‐donors:2‐(hydroxymethyl)pyridine(2‐(HOCH2)py),2‐(hydroxyethyl)pyridine(2‐(HOCH2CH2)py),2‐(2‐pyridyl)benzimidazole(pyBIm),di(2‐pyridyl)ke‐tone(py2CO).TheobtainedCu(II)complexeswerefullycharacterisedbyelementalanalysis,FTIR,EPR,UV‐Vis,single‐crystalX‐raydiffractionandHirshfeldsurfaceanalysis.Crystallographicandspectroscopicanalysesconfirmedchromophoresofbothmonomeric({CuN3O3}(1),{CuN2O4}(2),{CuN6}(4),{CuN4O2}(5))anddimericcomplex({CuN2O3}(3)).Mostoftheobtainedspeciespos‐sessedadistortedoctahedralenvironment,exceptdimer3,whichconsistedoftwocoppercentreswithsquarepyramidalgeometries.Thewater‐solublecompounds(1,3and5)wereselectedforbiologicaltesting.Theresultsofthestudyrevealedthatcomplex1insolutionsdisplayedbetterradicalscavengingactivitythancomplexes3,5andfreeligands.Therefore,complex1hasbeenselectedforfurtherstudiestotestitsactivityasanenzymemimetic.Thechosencompoundwastestedontheerythrocytelysateoftwogroupsofpatientsafterundergoingchemotherapyandchemoradiotherapy.Theeffectofthetestedcompound(1)onenzymeactivitylevels(TAS,SODandCAT)suggeststhattheselectedcomplexcanbetreatedasafunctionalmimeticoftheenzymes.
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Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites—A Comparative Study
PublicationThis article compares the properties of bacterial cellulose/fish collagen composites (BC/Col) after enzymatic and chemical cross-linking. In our methodology, two transglutaminases are used for enzymatic cross-linking—one recommended for the meat and the other proposed for the fish industry—and pre-oxidated BC (oxBC) is used for chemical cross-linking. The structure of the obtained composites is characterized by scanning electron...
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The bile salt/phospholipid ratio determines the extent of in vitro intestinal lipolysis of triglycerides: Interfacial and emulsion studies
PublicationThis study focused on the protein-stabilised triglyceride (TG)/water interfaces and oil-in-water emulsions, and explored the influence of varying molar ratios of bile salts (BSs) and phospholipids (PLs) on the intestinal lipolysis of TGs. The presence of these two major groups of biosurfactants delivered with human bile to the physiological environment of intestinal digestion was replicated in our experiments by using mixtures...
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Study on AR Application Efficiency of Selected iOS and Android OS Mobile Devices
PublicationCurrently, the number of scenarios for using AR (Augmented Reality) tools grows every year. Yet, there are still some open performance issues associated with this technology, related with the efficiency of the mobile device itself, including build-in components or the operation system. The purpose of this study was to investigate available augmented reality engines for mobile platforms. First, a review of current graphics and AR...
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DIAMENTY JAKO PRZYKŁAD INWESTYCJI ALTERNATYWNEJ
PublicationW artykule scharakteryzowano determinanty decydujące o powodzeniu inwestycji w diamenty. Cel artykułu to zbadanie, jak masa, czystość, szlif, kolor, jednostka certyfikująca i kształt wpływają na cenę diamentu. Aby określić wpływ tych cech na cenę diamentu, zbudowano pięć modeli ekonometrycznych. Dla realizacji postawionego celu badawczego wykorzystano charakterystyki dla ponad 265 tysięcy diamentów. W wyniku przeprowadzonej analizy...
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Determination of Odour Interactions in Gaseous Mixtures Using Electronic Nose Methods with Artificial Neural Networks
PublicationThis paper presents application of an electronic nose prototype comprised of eight sensors, five TGS-type sensors, two electrochemical sensors and one PID-type sensor, to identify odour interaction phenomenon in two-, three-, four- and five-component odorous mixtures. Typical chemical compounds, such as toluene, acetone, triethylamine, α-pinene and n-butanol, present near municipal landfills and sewage treatment plants were subjected...
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Heterogeneity of quaternary structure of glucosamine-6-phosphate deaminase from Giardia lamblia
PublicationThe oligoHis-tagged versions of glucosamine-6- phosphate deaminase from Giardia lamblia (GlmNagB-HisN, GlmNagB-HisC) were constructed and purified to hear homogeneity, and their kinetic and structural properties were compared to those of the wild-type enzyme (GlmNagB). Introduction of the oligoHis tag at the GlmNagB C-terminus resulted in almost complete loss of the catalytic activity, while the catalytic properties of GlmNagB-HisN...
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Expression of Deinococcus geothermalis Trehalose Synthase Gene in Escherichia coli and Its Enzymatic Properties
PublicationA novel trehalose synthase gene from Deinococcus geothermalis (DSMZ 11300) containing 1,692 bp reading-frame encoding 564 amino acids was amplified using PCR. The gene was ligated into pET30Ek/LIC vector and expressed after isopropyl alfa-D-thiogalactopyranoside induction in Escherichia coli BL21(DE3)pLysS. The recombinant trehalose synthase (DgeoTreS) containing a His6 tag at the C-terminus was purified by metal affinity chromatography...
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Suwerenność państwa i rządy prawa: kodyfikacja prawa prywatnego w Chinach
PublicationThe main issue of this article is a comparison of codification of private law in the Republic of China (1912–1949) with the current fi fth attempt to codify civil law in the People’s Republic of China, which is planned to be enacted in 2020. The aim of the paper is to prove that in both the most important factors for drafting new laws were not the internal needs of the state, but the necessity to regulate the...
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Fighting Administrative Corruption with Digital Government in Sub-Saharan Africa
PublicationAdministrative corruption is a pervasive problem and a major threat to economic and social development around the world, especially in Sub-Saharan Africa which lags behind other regions in various development indicators and is seen as one of the most corrupt regions globally. This paper examines a hypothesis that digital government – the use of digital technology to transform public administration organizations and their relationships...
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Charakterystyka frakcji oleju palmowego otrzymanych na drodze wielostopniowej krystalizacji
PublicationPrzeprowadzono wielostopniową frakcjonowaną krystalizację oleju palmowego z acetonu. Stosując temperatury krystalizacji od 25 do 0C, otrzymano siedem frakcji znacznie różniących się budową chemiczną i właściwościami fizycznymi. We frakcjach oznaczono skład kwasów tłuszczowych metodą GLC oraz zawartość triacylogliceroli metodą HPLC. Zbadano też właściwości fizyczne otrzymanych frakcji. Oznaczono zawartości fazy stałej metodą pulsacyjnego...
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Extraction and evaluation of gas-flow-dependent features from dynamic measurements of gas sensors array
PublicationGas analyzers based on gas sensors are the devices which enable recognition of various kinds of volatile compounds. They have continuously been developed and investigated for over three decades, however there are still limitations which slow down the implementation of those devices in many applications. For example, the main drawbacks are the lack of selectivity, sensitivity...
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Wyszukiwanie informacji z wykorzystaniem algorytmu Ontology Clustering by Directions
PublicationArtykuł opisuje algorytm Ontology Clustering by Directions. Algorytm ten ma na celu wspieranie użytkowników w formułowaniu ontologicznych zapytań. Ontologiczne zapytania służą do wydobywania informacji sformułowanych za pomocą ontologii opisanych np. językiem OWL. Artykuł przedstawia rodzaje języków wykorzystywanych do formułowania ontologicznych zapytań. W szczególności opisuje języki, które mają być przyjazne użytkownikom. Na...
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Zastosowania chromatografii wykluczania (SEC) w analityce technicznej i preparatyce – część I-sza.
PublicationW pracy scharakteryzowano założenia teoretyczne oraz mechanizm rozdzielania w warunkach chromatografii wykluczania (SEC). Przeanalizowano wady i zalety tej techniki rozdzielania. Przedstawiono przegląd zastosowań obejmujący wykorzystanie SEC w analityce technicznej oraz w preparatyce. SEC w warunkach liofilowych znajduje zastosowanie do rozdzielania mieszanin polimerów t.j. polistyren, polibutadien, poliizopren, poli(dimetylosiloksan),...
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Highly Reliable Passive RFID-Based Inductor–Capacitor Sensory System Strengthened by Solvatochromism for Fast and Wide-Range Lactate Detection
PublicationA passive radio frequency-identification-based inductor-capacitor (LC) lactate sensory system with a specific dye-containing interdigitated capacitor (IDC) in which the sensing signal is amplified by the solvatochromic effect is proposed. When a lactate solution contacts the IDC of the LC lactate sensor, the capacitance of the IDC changes, changing the resonance frequency of the sensor. This changes the oscillation frequency of...
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Microbe Cultivation Guidelines to Optimize Rhamnolipid Applications
PublicationIn the growing landscape of interest in natural surfactants, selecting the appropriate one for specific applications remains challenging. The extensive, yet often unsystematized, knowledge of microbial surfactants, predominantly represented by rhamnolipids (RLs), typically does not translate beyond the conditions presented in scientific publications. This limitation stems from the numerous variables and their interdependencies...
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Development of Gas Sensor Array for Methane Reforming Process Monitoring
PublicationThe article presents a new method of monitoring and assessing the course of the dry methane reforming process with the use of a gas sensor array. Nine commercially available TGS chemical gas sensors were used to construct the array (seven metal oxide sensors and two electrochemical ones). Principal Component Regression (PCR) was used as a calibration method. The developed PCR models were used to determine the quantitative parameters...
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An attempt to mechanistically explain the viscoelastic behavior of transparent epoxy/starch-modified ZnO nanocomposite coatings
PublicationThe effects of bare and starch-modified ZnO (ZnO-St) nanoparticles on viscoelastic and mechanical properties are studied by dynamic mechanical and tensile analyses. Transparent epoxy-based nanocomposite films are prepared by incorporating bare or starch-modified ZnO particles into the epoxy matrix. The results demonstrated that ZnO particles hindered the curing reactions and hence the final properties of the cured epoxy. As a result,...
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Magdalena Szuflita-Żurawska
PeopleHead of the Scientific and Technical Information Services at the Gdansk University of Technology Library and the Leader of the Open Science Competence Center. She is also a Plenipotentiary of the Rector of the Gdańsk University of Technology for open science. She is a PhD Candidate. Her main areas of research and interests include research productivity, motivation, management of HEs, Open Access, Open Research Data, information...
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Identification of Substrates of Cytoplasmic Peptidyl-Prolyl Cis/Trans Isomerases and Their Collective Essentiality in Escherichia Coli
PublicationProtein folding often requires molecular chaperones and folding catalysts, such as peptidyl-prolyl cis/trans isomerases (PPIs). The Escherichia coli cytoplasm contains six well-known PPIs, although a requirement of their PPIase activity, the identity of their substrates and relative enzymatic contribution is unknown. Thus, strains lacking all periplasmic and one of the cytoplasmic PPIs were constructed. Measurement of their PPIase...
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Marek Czachor prof. dr hab.
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Galerkin Finite Element Process for Entropy Production and Thermal Evaluation of Third-Grade Fluid Flow: A Thermal Case Study
Publication: A fluid’s moving class improves its heat transmission capability, as well as its rigidity, owing to multivariate molecule suspension. In this way, nanofluids are superior to common fluids. In this study, we evaluated the features of ease and heat transfer. Furthermore, we investigated permeable media, heat source, variable heat conductivity, and warm irradiation results. A mathematical technique known as the Galerkin finite element...
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Recombinant !ermostable AP Exonuclease from Thermoanaerobacter tengcongensis: Cloning, Expression, Purification, Properties and PCR Application
PublicationApurinic/apyrimidinic (AP) sites in DNA are considered to be highly mutagenic and must be corrected to preserve genetic integrity, especially at high temperatures. !e gene encoding a homologue of AP exonuclease was cloned from the thermophilic anaerobic bacterium Thermoanaerobacter tengcongensis and transformed into Escherichia coli. The protein product showed high identity (80%) to human Ape1 nuclease, whereas to E. coli exonuclease...
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Combined environmental testing device for picosatellites
PublicationAs access to space conditions becomes more available, both technically and economically, scientists’ interest in launching finer and more sophisticated experiments grows. To be qualified for launch, such experiments need to be carefully tested prior to the event. The tests should represent actual launch conditions as closely and in a detailed manner. Typical tests for payload include: vibration tests, thermal and vacuum tests,...
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Experiencing the Ocean the Paths for Urban Development of Sao Pedro do Estoril in Lisbon Metropolitan Area
PublicationThe areas of Cascais Municipality, one of the richest Municipalities in Portugal, located west to Lisbon, at the estuary of Tagus and the Atlantic Ocean coast is popular tourist destination. With its unique landscape it became the most attractive venue for spending vacations, for sport activities and leisure. It arose around the coastal town of Cascais, the former fishing village famous as a refuge for notables and claiming to...
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Problemy z oznaczaniem zawartości fazy stałej z zastosowaniem normy ISO 8292 = Problems with the solid fat content determination using the ISO 8292 standard.
PublicationZawartość fazy stałej (SFC) jest parametrem charakteryzującym właściwości fizyczne i użytkowe tłuszczów stałych. Otrzymanie prawidłowego wyniku zależy od zastosowania odpowiedniej procedury termostatowania próbki przed pomiarem, a ta z zależy od rodzaju badanego tłuszczu. Przed oznaczeniem SFC wszystkie TAG, które są stałe w temperaturze 0oC powinny wykrystalizować, a tłuszcz osiągnąć trwałą formę krystaliczną. Pierwsza norma IUPAC...