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Wyniki wyszukiwania dla: MOTOR PROTEINS
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Highly Specific Substrates of Proteinase 3 Containing 3-(2-Benzoxazol-5-yl)-l-alanine and Their Application for Detection of This Enzyme in Human Serum
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Design, Chemical Synthesis and Kinetic Studies of Trypsin Chromogenic Substrates Based on the Proteinase Binding Loop of Cucurbita maxima Trypsin Inhibitor (CMTI-III)
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In vitro studies of polyphenols, antioxidants and other dietary indices in kiwifruit (Actinidia deliciosa)
PublikacjaOpisano wyniki badań poziomu protein oraz potencjału przeciwutleniającego w próbkach owoców kiwi, przechowywanych i dojrzewających w atmosferze etylenu ( w ciągu pierwszych 10 dni). Próbki owoców kiwi podzielono (w sposób przypadkowy) na dwie grupy:-próbki nie poddawane obróbce,-próbki poddawane obróbce (dojrzewanie w atmosferze etylenu).W próbkach oznaczano między innymi: wolne cukry, proteiny, polifenole oraz zdolność przeciwutleniającą....
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Speed sensorless ac drive with inverter output filter and fault detection using load torque signal
PublikacjaIn this paper, a sensorless induction motor (IM) drive using speed observer system is presented. The system includes load torque computation for gear fault detection. Nonlinear control method is adopted for controlling the motor over a wide speed range. An LC filter for smoothing current and voltage waveforms is used at the output of the voltage inverter. The use of filter imposes more building of the used observer to avoid using...
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DIAGNOSTYKA ŁOŻYSK SILNIKA INDUKCYJNEGO PRZY UŻYCIU POMIARU PRĄDU ZASILAJĄCEGO I WIBRACJI
PublikacjaW artykule przedstawione zostały wyniki badań diagnostycznych łożysk silnika indukcyjnego z zastosowaniem metody pomiaru prądu zasilającego oraz wibracji. Poruszone zostały problemy dotyczące poszukiwania nowej metody opartej na pomiarze i analizie prądu, która w znacznym stopniu ułatwiłaby szybkie i sprawne znalezienie uszkodzenia w maszynie, bez konieczności bezpośredniego dostępu do badanego urządzenia. Zaprezentowano rezultaty...
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Ageing-Time Dependent Changes of Angiotensin I-Converting Enzyme-Inhibiting Activity of Protein Hydrolysates Obtained from Dry-Cured Pork Loins Inoculated with Probiotic Lactic Acid Bacteria
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Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces
PublikacjaIn this work, we study the electrodes with a periodic matrix of gold particles pattered by titanium dimples and modified by 3-mercaptopropionic acid (MPA) followed by CD147 receptor grafting for specific impedimetric detection of SARS-CoV-2 viral spike proteins. The synergistic DFT and MM/MD modeling revealed that MPA adsorption geometries on the Au–Ti surface have preferential and stronger binding patterns through the carboxyl...
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Significant Decrease in Glycated Hemoglobin, 2h-Post-Load Glucose and High-Sensitivity C-Reactive Protein Levels in Patients with Abnormal Body Mass Index after Therapy with Manual Lymphatic Drainage
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Measurements of drive system properties of the "Magnetic SpeedLine" ultra-fast robot prototype for IML labeling
Dane BadawczeTests of the drive system of the ultra-fast robot "SpeedLine magnetic" for labeling with the IML method were carried out in order to analyze the influence of the operating parameters of the drive system on the correctness and quality of the drive system.
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A New Expression System Based on Psychrotolerant Debaryomyces macquariensis Yeast and Its Application to the Production of Cold-Active β-D-Galactosidase from Paracoccus sp. 32d
PublikacjaYeasts provide attractive host/vector systems for heterologous gene expression. The currently used yeast-based expression platforms include mesophilic and thermotolerant species. A eukaryotic expression system working at low temperatures could be particularly useful for the production of thermolabile proteins and proteins that tend to form insoluble aggregates. For this purpose, an expression system based on an Antarctic psychrotolerant...
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Molecular Strategy for Survival at a Critical High Temperature in Eschierichia coli
PublikacjaThe molecular mechanism supporting survival at a critical high temperature (CHT) in Escherichia coli was investigated. Genome-wide screening with a single-gene knockout library provided a list of genes indispensable for growth at 47°C, called thermotolerant genes. Genes for which expression was affected by exposure to CHT were identified by DNA chip analysis. Unexpectedly, the former contents did not overlap with the latter except...
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Koncepcja i modelowanie wysokoobrotowego napędu elektrycznego turbosprężarki. Zastosowanie w ogniwach paliwowych pojazdów samochodowych
PublikacjaAbstract: Electrical vehicles powered by hydrogen fuel cells become a potential alternative to conventional vehicles. The polymer electrolyte membrane (PEM) are typical fuel cells used in fuel cell vehicle (FCV). The global efficiency of a PEM fuel cell stack is greatly impacted by the use of a motorized compressor for the air supply system. The motor-compressor set-up may consume up to 19 % of the energy supplied by the PEM fuel...
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In vitro affinity of Deinococcus radiodurans MutS towards mismatched DNA exceeds that of its orthologues from Escherichia coli and Thermus thermophilus
PublikacjaThe mismatch binding protein MutS is responsible for the recognition of mispaired and unpaired bases, which is the initial step in DNA repair. Among the MutS proteins most extensively studied in vitro are those derived from Thermus thermophilus, Thermus aquaticus and Escherichia coli. Here, we present the first report on the in vitro examination of DNA mismatch binding activity of MutS protein from Deinococcus radiodurans and confront...
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Pompownie dużej mocy z falownikiem wędrującym średniego napięcia
PublikacjaArtykuł zawiera rys rozwoju napędów przekształtnikowych w stacjach pomp, w szczególności regulacji wydatku kilku pomp, po kolei, przez jeden przemiennik częstotliwości. Omówiono podstawy teoretyczne zjawiska pola niestłumionego i napięcia resztkowego silników, które utrudniają szybkie przełączania silnika pompy pomiędzy zasilaniem regulowanym przez falownik a zasilaniem z sieci. Przedstawiono zasady transferu (przełączania) synchronicznego...
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Satelitowy agregat pompowy
PublikacjaW artykule opisano najnowszą konstrukcję satelitowego agregatu pompowego charakteryzującego się tym, że pompa satelitowa jest umieszczona wewnątrz silnika elektrycznego i zasysa ciecz poprzez otwór wydrążony osiowo w wale silnika. Zastosowana w agregacie pompa satelitowa jest nowatorską konstrukcją zawierającą mechanizm satelitowy o odwróconej kinematyce znamienny tym, że planeta (wirnik) oraz płyty kompensacyjne (rozrządu) są...
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Ligand-Modified Boron-Doped Diamond Surface: DFT Insights into the Electronic Properties of Biofunctionalization
PublikacjaWith the increasing power of computation systems, theoretical calculations provide a means for quick determination of material properties, laying out a research plan, and lowering material development costs. One of the most common is Density Functional Theory (DFT), which allows us to simulate the structure of chemical molecules or crystals and their interaction. In developing a new generation of biosensors, understanding the nature...
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Mathemathical model of radial passive magnetic bearing
PublikacjaIn the article mathematical radial model of passive magnetic bearings to ocean engineering units has been presented. The units application in underwater thrusters should increase propulsion systems durability and gives lower maintenance costs. The rotor of electric motor is hanging in magnetic field taken from radial passive magnetic bearings. However axial direction maintenance must be controlled with electromagnets and their...
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Dobór parametrów silnika indukcyjnego dużej mocy
PublikacjaW artykule przedstawiono trzy typy statycznych modeli matematycznych silników klatkowych oraz metodę estymacji parametrów, przy wykorzystaniu algorytmów genetycznych. Korzystając z kryteriów: suma kwadratów, suma wartości bez-względnych oraz całkowego, oceniono przydatność badanych modeli. Opracowane modele matematyczne zostały wykorzystane przy doborze algorytmów sterownia sterów strumieniowych. Po-kazano metodykę doboru parametrów...
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Implementation of Extended Kalman Filter with Optimized Execution Time for Sensorless Control of a PMSM Using ARM Cortex-M3 Microcontroller
PublikacjaThis paper addresses the implementation and optimization of an Extended Kalman Filter (EKF) for the Permanent Magnet Synchronous Motor (PMSM) sensorless control using an ARM Cortex-M3 microcontroller. A various optimization levels based on arithmetic calculation reduction was implemented in ARM Cortex-M3 microcontroller. The execution time of EKF estimator was reduced from 260.4 μs to 37.7 μs without loss of accuracy. To further...
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A Review of Reduction Methods of Impact of Common-Mode Voltage on Electric Drives.
PublikacjaIn this survey paper, typical solutions that focus on the reduction in negative effects resulting from the common-mode voltage influence in AC motor drive applications are re-examined. The critical effectiveness evaluation of the considered methods is based on experimental results of tests performed in a laboratory setup with an induction machine fed by an inverter. The capacity of a common-mode voltage level reduction and voltage...
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Cloning, Expression and Purification of Wild-Type Trehalose Synthase from Deinococcus geothermalis
PublikacjaThe aim of this study was isolation and cloning of trehalose synthase gene derived from extremophilic microorganism to the expression vectors in the Tabor-Studier system and its expression in Rosetta(DE3)pLysS Escherichia coli cells. The second phase of the study consisted of proteins purification using an initial denaturation of host proteins and salting-out proteins by ammonium sulfate.
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Exploiting the S4–S5 Specificity of Human Neutrophil Proteinase 3 to Improve the Potency of Peptidyl Di(chlorophenyl)-phosphonate Ester Inhibitors: A Kinetic and Molecular Modeling Analysis
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Joanna Raczek dr inż.
OsobyWykształcenie 1997 -- 2001 Studia inżynierskie, Wydział Fizyki Technicznej i Matematyki Stosowanej, Politechnika Gdańska. Kierunek: Matematyka, specjalność: Matematyka Stosowana. 2001 -- 2003 Studia magisterskie, Wydział Fizyki Technicznej i Matematyki Stosowanej, Politechnika Gdańska. Kierunek: Matematyka, specjalność: Matematyka Stosowana. 2000 -- 2004 Studia inżynierskie, Wydział Elektroniki, Informatyki i Telekomunikacji,...
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Medium-Voltage Drives: Challenges and existing technology
PublikacjaThe article presents an overview of state-of-art solutions, advances, and design and research trends in medium-voltage (MV) drive technologies - and also discusses the challenges and requirements associated with the use of such drives. The choice and deployment of MV drives in industries are associated with numerous requirements related to the front-end converter (grid side) and inverter (machine side). The focus is on solutions...
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Enhancing colloidal stability of nanodiamond via surface modification with dendritic molecules for optical sensing in physiological environments
PublikacjaPre-treatment of diamond surface in low-temperature plasma for oxygenation and in acids for carboxylation was hypothesized to promote the branching density of the hyperbranched glycidol polymer. This was expected to increase the homogeneity of the branching level and suppress interactions with proteins. As a result, composite nanodiamonds with reduced hydrodynamic diameters that are maintained in physiological environments were...
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Boron-doped carbon nanowalls for fast and direct detection of cytochrome C and ricin by matrix-free laser desorption/ionization mass spectrometry
PublikacjaDetecting proteins via surface assisted laser desorption/ionization mass spectrometry (SALDI-MS) method is still highly challenging, and only few examples of nanomaterials have been demonstrated to perform such detection so far. In this study, carbon nanowalls (CNWs), vertically aligned graphene sheet-based materials, presenting specific morphology, dimensions, and boron doping levels have shown improved performances for both qualitative...
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Expression of a GDSL esterase from Pseudomonas sp. S9 in Pichiapastoris
PublikacjaCold active lipolytic enzymes are promising to replace the conventional enzymes processes of biotechnological industries. One of the most important feature of the cold-active lipases and esterases is that they offer economic benefits through energy saving. In general, they exhibit high activity at low temperatures and low thermostability at moderate temperatures. Lipolytic enzyme EstS9 was isolated from Pseudomonas sp. S9. A multiple...
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Multicopy Suppressor Analysis of Strains Lacking Cytoplasmic Peptidyl-Prolyl cis/trans Isomerases Identifies Three New PPIase Activities in Escherichia coli That Includes the DksA Transcription Factor
PublikacjaConsistent with a role in catalyzing rate-limiting step of protein folding, removal of genes encoding cytoplasmic protein folding catalysts belonging to the family of peptidyl-prolyl cis/trans isomerases (PPIs) in Escherichia coli confers conditional lethality. To address the molecular basis of the essentiality of PPIs, a multicopy suppressor approach revealed that overexpression of genes encoding chaperones (DnaK/J and GroL/S),...
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Fibrosarcoma, NOS - Male, 57 - Tissue image [420073001206531]
Dane BadawczeThis is the histopathological image of CONNECTIVE, SUBCUTANEOUS AND OTHER SOFT TISSUES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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In silico epitope prediction of Borrelia burgdorferi sensu lato antigens for the detection of specific antibodies
PublikacjaDespite many years of research, serodiagnosis of Lyme disease still faces many obstacles. Difficulties arise mainly due to the low degree of amino acid sequence conservation of the most immunogenic antigens among B. burgdorferi s.l. genospecies, as well as differences in protein production depending on the environment in which the spirochete is located. Mapping B-cell epitopes located on antigens allows for a better understanding...
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Impact of high-pressure processing on the bioactive compounds of milk - A comprehensive review
PublikacjaHigh-pressure processing (HPP) is a promising alternative to thermal pasteurization. Recent studies highlighted the effectivity of HPP (400–600 MPa and exposure times of 1–5 min) in reducing pathogenic microflora for up to 5 logs. Analysis of modern scientific sources has shown that pressure affects the main components of milk including fat globules, lactose, casein micelles. The behavior of whey proteins under HPP is very important...
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Error analysis of calculating average d-q current components using Regular Sampling and Park transformation in FOC drives
PublikacjaIn electric drives using voltage source inverters, motor currents contain ripple component resulting from pulse-width modulated (PWM) voltage. The frequency range of the ripples is much higher than the bandwidth of current control. Therefore the control is performed on the basis of a fundamental current component, i.e. average value with the averaging time being the PWM period. In majority of cases the average current is measured...
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The methodology of design of axial clearances compensation unit in hydraulic satellite displacement machine and their experimental verification
PublikacjaA new methodology of calculating the dimensions of the axial clearance compensation unit in the hydraulic satellite displacement machine is described in this paper. The methods of shaping the compensation unit were also proposed and described. These methods were used to calculate the geometrical dimensions of the compensation field in an innovative prototype of a satellite hydraulic motor. This motor is characterized by the fact...
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Medium voltage drives - challenges and requirements
PublikacjaThe design and application of medium voltage (MV) drives are associated with different requirements related to the line side and motor side of the drive. This paper presents a brief review of the existing challenges and requirements for employing medium voltage drives in various industries. Line side, motor side, and converterproblems and requirements are described. The aim of the paper is to review the existing problems to allow...
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Toxoplasma godnii recombinant chimeric antigens - IgM and IgG ELISAs - mouse serum samples
Dane BadawczeThis study presents an evaluation of tetravalent recombinant chimeric proteins containing fragments of the Toxoplasma gondii antigens, SAG2, SAG1, GRA1, GRA2, MIC1, MAG1, ROP1 and AMA1, as potential replacements of a the soluble, whole-cell tachyzoite lysate (TLA) used in serological assays. Recombinant chimeric proteins (SAG1-MIC1-MAG1-GRA2, SAG2-GRA1-ROP1-GRA2,...
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Toxoplasma gondii recombinant chimeric antigens - IgG and IgM ELISAs - human serum samples
Dane BadawczeThis study presents an evaluation of tetravalent recombinant chimeric proteins containing fragments of the Toxoplasma gondii antigens, SAG1, SAG2, GRA1, GRA2, MIC1, MAG1, ROP1 and AMA1, as potential replacements of a the soluble, whole-cell tachyzoite lysate (TLA) used in serological assays. Recombinant chimeric proteins (SAG1-MIC1-MAG1-GRA2, SAG2-GRA1-ROP1-GRA2,...
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In vitro antioxidative and binding properties of phenolics in traditional, citrus and exotic fruits
PublikacjaMany polyphenols bind proteins, therefore our research was focused on the potential of protein binding to polyphenols of investigated fruits and their health-related effects. The contents of polyphenols and related antioxidant activities of traditional, citrus and exotic fruits were compared. The presence of polyphenols (flavonoids and phenolic acids) in the investigated samples and their interaction with human serum albumin (HSA)...
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Cyanogenic Compounds and Estrogen Disruptors
PublikacjaBalanced diet consists largely of plants containing cyanogenic compounds in the form of the more common cyanogenic glycosides and sometimes lipids. Maize, wheat, rye, apples, barley, oats, sugar cane and yet many other plants consumed by humans contain cyanogenic compounds. However the risk of poisoning is negligible as it is very easy to remove the toxic HCN by grinding and drying in air or soaking in water and an additional...
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Low-power microwave-induced fabrication of functionalised few-layer black phosphorus electrodes: A novel route towards Haemophilus Influenzae pathogen biosensing devices
PublikacjaIn this paper, various passivation schemes were applied at few-layer black phosphorus (FLBP) to achieve covalent functionalisation with 4-azidobenzoic acid, improving its electrochemical response intended for analytical and biosensing applications. The thermal and microwave assisted modification procedures in toluene and dime-thylformamide resulted in high reversibility of reactions on functionalised FLBP using a ferricyanide/ferrocya-nide...
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Identification of antigen Ag43 in uropathogenic Escherichia coli Dr+ strains and defining its role in the pathogenesis of urinary tract infections
PublikacjaUrinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are among the most common bacterial infectious diseases in the developed world. The urovirulence of UPEC is mainly associated with the surface-exposed fimbrial adhesins and adhesins of the autotransporter (AT) family. The best studied of theses proteins is antigen 43 (Ag43) mediating cell aggregation, adhesion and biofilm development as the causes of...
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Gracjana Klein-Raina dr hab.
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Load effect impact on the exploitation of concrete machine foundations used in the gas and oil industry
PublikacjaMachine foundations is a critical topic in the gas and oil industry, which design and exploitation require extensive technical knowledge. Machine foundations are the constructions which are intended for mounting on it a specific type of machine. The foundation has to transfer dynamic and static load from machine to the ground. The primary difference between machine foundations and building foundations is that the machine foundations...
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[NCh-bio] Advances in Biotechnology: Protein Folding and Assembly
Kursy Online{mlang pl} Dyscyplina: nauki chemiczne Zajęcia obowiązkowe dla doktorantów I i II roku z obszaru biotechnologii Prowadzący: dr hab. Gracjana Klein-Raina, prof. PG Liczba godzin: 15 Forma zajęć: wykład {mlang} {mlang en} Discipline: chemical sciences Obligatory course for 1st and 2nd-year PhD students from biotechnology area Academic teachers: dr hab. Gracjana Klein-Raina, prof. PG Total hours of training: 15 teaching...
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Diagnostyka łożysk silnika indukcyjnego na podstawie prądu zasilającego przy użyciu sztucznych sieci neuronowych
PublikacjaW artykule zawarto wyniki badań dotyczące diagnostyki łożysk silnika indukcyjnego na podstawie pomiarów prądu zasilającego z wyko-rzystaniem sztucznych sieci neuronowych. Zaprezentowano wyniki uczenia sieci oraz rezultaty testów przeprowadzonych na danych spoza zbioru uczącego. Badania wykonane zostały na obiektach z celowo wprowadzonymi uszkodzeniami łożysk. Przedstawiona nowa koncepcja zakłada użycie zestawu sieci neuronowych...
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Design, synthesis, and biological evaluation of tetrahydroquinolinones and tetrahydroquinolines with anticancer activity
PublikacjaColorectal cancer (CRC) is the most commonly diagnosed cancer in Europe and the United States and the second leading cause of cancer related mortality. A therapeutic strategy used for the treatment of CRC involves targeting the intracellular levels of reactive oxygen species (ROS). In this study, we synthesized a series of novel tetrahydroquinolinones and assessed their ability to inhibit CRC growth and proliferation by evoking...
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Design of Intelligent Low-Voltage Load Switch for Remote Control System in Smart Grid
PublikacjaCurrent low-voltage load switches do not support remote disconnect/connect and real-time monitoring of a disconnect/connect state. Addressing to these issues, this paper presents a low-voltage load switch for a smart remote control system, which uses a one-chip microcontroller board and a DC step motor drive mechanism and provides the feedback on the switch status also. Arrears disconnect and full-pay connect control is implemented...
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A predictive estimation based control strategy for a quasi-resonant dc-link inverter
PublikacjaIn this paper the predictive estimation based control strategy for a quasi-resonant dc link inverter (PQRDCLI) is developed. Instead of direct measurement of dc link input inverter current – its estimation with one step prediction is applied. The PQRDCLI fed induction motor, controlled with a predictive current estimation stabilized inverter output voltage slopes independently of load. Moreover, reduction of overvoltage spikes...
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The methodology of design of satellite working mechanism of positive displacement machine
PublikacjaIn this paper is described a methodology of design of satellite mechanism consisting of two noncircular gears (externally toothed rotor and internally toothed curvature) and circular gears (satellites). In the presented methodology is assumed that the rotor pitch line is known, and the curvature pitch line is necessary to designate. The presented methodology applies to mechanisms for which the number of the curvature humps is at...
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Diagnostyka off-line stanu izolacji uzwojeń silników 6 kV z wykorzystaniem obrazowania akustycznego wyładowań niezupełnych
PublikacjaW zakładach przemysłowych, w których kluczowe procesy produkcyjne są realizowane z wykorzystaniem silników średniego napięcia, dla których diagnostyka izolacji uzwojeń ma istotne znaczenie. W tym celu wykonuje się ocenę porównawczą wyników pomiarów wyładowań niezupełnych (WNZ) wykonywanych dla jednostek tego samego typu, a jej skuteczność w znacznej mierze wynika z wieloletniego doświadczenia diagnosty. Dla opracowania wyników...
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Formowanie wektorów napięcia wyjściowego w pięciofazowych falownikach napięcia
PublikacjaW falownikach pięciofazowych, załączenie wektorów aktywnych powoduje jednoczesne formowane dwóch, zależnych od siebie wektorów napięcia wyjściowego. Sterowanie pięciofazowymi silnikami indukcyjnymi wymaga, aby wektory te miały ściśle określone długości i położenia. Niezależne formowanie dwóch wektorów napięcia o parametrach określonych w układzie sterowania silnikiem wymaga zastosowania odpowiedniej strategii Modulacji Szerokości...