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Search results for: DNA OXIDATIVE DAMAGE
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Numerical investigation on behaviour of cylindrical steel tanks during mining tremors and moderate earthquakes
PublicationCylindrical steel tanks are important components of industrial facilities. Their safety becomes a crucial issue since any failure may cause catastrophic consequences. The aim of the paper is to show the results of comprehensive FEM numerical investigation focused on the response of cylindrical steel tanks under mining tremors and moderate earthquakes. The effects of different levels of liquid filling, the influence of non-uniform...
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Coda wave interferometry in monitoring the fracture process of concrete beams under bending test
PublicationEarly detection of damage is necessary for the safe and reliable use of civil engineering structures made of concrete. Recently, the identification of micro-cracks in concrete has become an area of growing interest, especially using wave-based techniques. In this paper, a non-destructive testing approach for the characterization of the fracture process was presented. Experimental tests were made on concrete beams subjected to mechanical...
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X-Ray Computer Tomography Study of Degradation of the Zircaloy-2 Tubes Oxidized at High Temperatures
PublicationThe investigations of high-temperature oxidation of zirconium alloys, applied for fuel pellets in nuclear power plants, are usually limited to oxidation kinetics, phase transformations and microstructural characterization. The purpose of this research was to characterize the degradation phenomena occurring within oxide layer and at the interface oxide/metal, on internal and external Zircaloy-2 tube...
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Diagnostic system of wheeled tractors detecting four defect's categories
PublicationIn a classical approach to damage diagnosis, the technical condition of an analyzed machine is identified based on the measured symptoms, such as performance, thermal state or vibration parameters. In wheeled tractor the fundamental importance has monitoring and diagnostics during exploitation concerning technical inspection and fault element localizations. The main functions of a diagnostic system are: monitoring tractor components...
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Uszkodzenia korozyjne wewnątrz stalowych zbiorników na paliwa płynne. Co najbardziej zagraża?
PublicationScharakteryzowano przyczyny korozji w poszczególnych strefach wewnętrznej powierzchni płaszcza, dna i dachu zbiornika stalowego na paliwa płynne. Scharakteryzowano uszkodzenia wywołane korozją wewnątrz zbiornika.
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Molecular dynamics simulation and MM-PBSA analysis of Oxytricha nova TEBP ternary complex with ssDNA
PublicationPraca dotyczy badań metodami chemii obliczeniowej właściwości elektrostatycznych i termodynamicznych kompleksu telomerowego białka TEBP pochodzącego z organizmu Oxytricha z jednoniciowym telomerowym DNA (ssDNA).
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Awaria dwupłaszczowego zbiornika stalowego na gorące media
PublicationPodano przyczyny uszkodzenia dna zbiornika dwupłaszczowego przeznaczonego do magazynowania asflatu w temperaturze ok. 200 C. Wskazano na konieczność wprowadzenia zmian w konstrukcji zbiornika.
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Experimental dynamic study on a timber-frame house using shaking table
PublicationThe aim of this paper is to show the results of the shaking table experimental study concerning the behaviour of the model of the timber-frame house under earthquake excitation and impact loading. In order to determine the basic dynamic properties of the undamaged structure, the free vibration and the sweep-sine tests were first conducted. Then, the model was excited using the time history record of the El Centro earthquake of...
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Internal imaging of concrete fracture based on elastic waves and ultrasound computed tomography
PublicationThe condition assessment of concrete structures belongs to the greatest challenges of non-destructive testing. Monitoring the fracture process of concrete and detecting cracks at the earliest possible stage is a vital aspect to ensure the safety of civil engineering objects. The use of ultrasound tomography enables imaging the internal structure of a tested element. This study aims at the visualization of fracture damage in concrete...
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Performance of isotropic constitutive laws in simulating failure mechanisms in scaled RC beams
PublicationResults of numerical calculations of reinforced concrete (RC) beams are presented. Based on experimental results on longitudinally reinforced specimens of different sizes and shapes are investigated. Four different continuum constitutive laws with isotropic softening are used: one defined within continuum damage mechanics, an elasto-plastic with the Rankine criterion in tension and the Drucker-Prager criterion in compression, a...
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Reference-free determination of debonding length in reinforced concrete beams using guided wave propagation
PublicationThis paper presents theoretical and experimental investigations of guided wave propagation in reinforced concrete beams, with pre-existing debonding between steel rebars and concrete blocks, for the purpose of damage detection. The primary aim of these investigations was a detailed analysis of the possible applications of wave propagation in single and multiple debonding detection in reinforced concrete structures and reference-free...
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A strong preference for the TA/TA dinucleotide step discovered for an acridine-based, potent antitumor dsDNA intercalator, C-1305: NMR-driven structural and sequence-specificity studies
PublicationTriazoloacridinone C-1305, a potent antitumor agent recommended for Phase I clinical trials, exhibits high activity towards a wide range of experimental colon carcinomas, in many cases associated with complete tumor regression. C-1305 is a well-established dsDNA intercalator, yet no information on its mode of binding into DNA is available to date. Herein, we present the NMR-driven and MD-refined reconstruction of the 3D structures...
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Construction and purification of his6-Thermus thermophilus MutS protein.
PublicationGen mutS z termofilnej bakterii Thermus thermophilus zamplifikowano przy użyciu PCR, sklonowano i przeprowadzono jego ekspresję w E. coli. Rekombinantowe białko MutS zawierające oligohistydynową domenę na N-końcu oczyszczono w jednoetapowej procedurze przy wykorzystaniu chromatografii powinowactwaNi(2+). Zdolność do rozpoznania niekomplementarności białka his(6)-MutS potwierdzono w eksperymentach ochrony DNA przed trawieniem...
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RecA Proteins from Deinococcus geothermalis and Deinococcus murrayi - Cloning, Purification and Biochemical Characterisation
PublicationEscherichia coli RecA plays a crucial role in recombinational processes, the induction of SOS response and mutagenic lesion bypasses. It has also been demonstrated that RecA protein is indispensable when it comes to the reassembly of shattered chromosomes in gamma-irradiated Deinococcus radiodurans, one of the most radiation-resistant organisms known. Moreover, some functional differences between E. coli and D. radiodurans RecA...
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Recombinant !ermostable AP Exonuclease from Thermoanaerobacter tengcongensis: Cloning, Expression, Purification, Properties and PCR Application
PublicationApurinic/apyrimidinic (AP) sites in DNA are considered to be highly mutagenic and must be corrected to preserve genetic integrity, especially at high temperatures. !e gene encoding a homologue of AP exonuclease was cloned from the thermophilic anaerobic bacterium Thermoanaerobacter tengcongensis and transformed into Escherichia coli. The protein product showed high identity (80%) to human Ape1 nuclease, whereas to E. coli exonuclease...
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Multipoint Ultrasonic Diagnostics System of Prestressed T-Beams
PublicationThis paper is devoted to the application of ultrasonic wave propagation phenomena for the diagnostics of prestressed, concrete, bridge T-beams. A multi-point damage detection system is studied with use of numerically obtained data. The system is designed to detect the presence of the material discontinuities as well as their location.
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3d Visualization of Seabed from Multibeam Sonar Records Using TriangularIrregular Mesh. W: [CD-ROM] Forum Acusticum Sevilla 2002. 3rd European Con-gres on Acoustics. XXXIII Spanish Congress on Acoustics-TECNIACUSTICA 2002.European and Japanese Symposium on Acoustics. Sevilla, Spain 16-20 Septem- ber 2002. Madrid: Soc. Espanola de Acustica**2002 vol. 33, [6 s.] 4 rys. bibliogr. 6 poz. ISBN: 84-87985-06-8 Special Issue of the Journal Revista de Acustica, vol.33, ano 2002. Trójwym. wizual.dna mors. z danych pochodz. z echosond wielowiąz. z wykorz.niergul.siat.TIN.
PublicationPrzedstawiono sposób trójwymiarowej wizualizacji dna morskiego przy wyko-rzystaniu siatki nieregularnej (TIN) otrzymanej w wyniku triangularyzacjioraz porównano otrzymane wyniki z rzeczywistymi pomiarami batymetrycznymidna Bałtyku Południowego.
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Towards Knowledge Sharing Oriented Adaptive Control
PublicationIn this paper, we propose a knowledge sharing oriented approach to enable a robot to reuse other robots' knowledge by adapting itself to the inverse dynamics model of the knowledge-sharing robot. The purpose of this work is to remove the heavy fine-tuning procedure required before using a new robot for a task via reusing other robots' knowledge. We use the Neural Knowledge DNA (NK-DNA) to help robots gain empirical knowledge and...
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BUDOWA I AKTYWNOŚĆ BIAŁEK NALEŻĄCYCH DO RODZINY TET
PublicationBiałka należące do rodziny TET (Tet1, Tet2, Tet3) są enzymami wykazującymi aktywność względem 5-metylocytozyny, która jest ważnym markerem epigenetycznym odgrywającym kluczową rolę w trakcie rozwoju organizmu i mającym istotny wpływ na poziom ekspresji genów. Białka TET przekształcają 5-metylocytozynę do 5-hydroksymetylocytozyny, 5-formylocytozyny i 5-karboksylocytozyny w trakcie następujących po sobie trzech reakcji utleniania....
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Photosensitive and pH-dependent activity of pyrazine-functionalized carbazole derivative as promising antifungal and imaging agent
PublicationCarbazole skeleton plays a significant role as a structural scaffold of many pharmacologically active compounds. Pyrazine-functionalized carbazole derivative was constructed by coupling 2-amino-5-bromo-3-methylaminepyrazine (ABMAP) into 3 and 6 positions of the carbazole ring. Multi-experimental methods were used, e.g., potentiometric, spectroscopic (ATR, UV, XRD powder,1H and13C NMR), electrochemical (cyclic voltammetry), and...
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Przeciwnowotworowe działanie akrydyn
PublicationOdkąd wykazano przeciwbakteryjne i przeciwwirusowe właściwości pochodnych akrydyny podjęto wiele prób modyfikacji chemicznej cząsteczki w celu znalezienia pochodnej charakteryzującej się wysoką aktywnością przeciwnowotworową. Wybór akrydyn jako potencjalnych leków przeciwnowotworowych podyktowany był przede wszystkim udowodnioną zdolnością tych związków do oddziaływania z DNA. W terapii przeciwnowotworowej, ze względu na brak istotnych...
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3D reconstruction seafloor from side-scan records
PublicationArtykuł przedstawia sposób wykorzystania języka opisu wirtualnej rzeczywistości (VRML) do trójwymiarowej wizualizacji dna morskiego. W szczególności zaprezentowano techniki rekonstrukcji trójwymiarowego obrazu z danych pochodzących z sonaru bocznego.
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Experimental Study on Pounding between Structures during Damaging Earthquakes
PublicationMutual pounding between structures during earthquakes may cause serious structural damage. The aim of this paper is to show the results of a shaking table experimental study focused on pounding between structures in series under several earthquake excitations. The experiments were performed using three tower models with different configurations and different gap distances between them. In the first stage of the study, one rigid...
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Investigation on domestic fruits seed oils in personal care emulsion systems
PublicationThe use of fruit seed oils in personal care products is of significance to both their function and image. Poland is an important processor of fruit products within the EU, and thus has a large availability of seeds from domestic fruits, which are normally considered to be a waste material. Unfortunately, current literature is scarce of the suitability of these oils for topical use in the form of cosmetic emulsions. Published data...
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Virtual Engineering Factory: Creating Experience Base for Industry 4.0
PublicationABSTRACT In recent times, traditional manufacturing is upgrading and adopting Industry 4.0, which supports computerization of manufacturing by round-the-clock connection and communica- tion of engineering objects. Consequently, Decisional DNA- based knowledge representation of manufacturing objects, processes, and system is achieved by virtual engineering objects (VEO), virtual engineering processes (VEP), and virtual engineering...
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Certain family of analytical solutions of nonlinear von Neumann equations
PublicationIn this paper we present a slight generalization of certain type of Darboux transformation, that may be used sub-sequently in a convenient way. This method allows to obtain families of solutions of nonlinear von Neumann equations, that are used in particular in DNA modeling.
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PARP inhibition potentiates the cytotoxic activity of C-1305, a selective inhibitor of topoisomerase II, in human BRCA1-positive breast cancer cells
PublicationTwo cellular proteins encoded by the breast and ovarian cancer type 1 susceptibility (BRCA1 and BRCA2) tumor suppressor genes are essential for DNA integrity and the maintenance of genomic stability.Approximately 5-10% of breast and ovarian cancers result from inherited alterations or mutations in these genes.Remarkably, BRCA1/BRCA2-deficient cells are hypersensitive to selective inhibition of poly(ADPribose) polymerase 1 (PARP-1),...
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Typing of Candida isolates from patients with invasive infection and concomitant colonization
PublicationWe investigated the relationship between colonizing and invasive isolates from patients with candidaemia. Molecular typing was performed using random amplification of polymorphic DNA (RAPD) and multilocus sequence typing (MLST). We found MLST to be sufficient for typing Candida isolates, and that surveillance cultures are helpful in predicting concomitant invasive isolates, but not necessarily the pathogen involved in subsequent...
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Zakażenia bakteryjne w przewlekłym zapaleniu ucha środkowego - ocena przydatności diagnostyki molekularnej opartej o technikę PCR. Bacterial infection in chronic otitis media - assessment of usefulness of molecular diagnostic based in PCR method.
PublicationIdentyfikacja zakażeń w przewlekłym zapaleniu ucha środkowego jest trudna, o czym świadczą wysokie odsetki ujemnych wyników badań bakteriologicznych. Przyczyną tego jest trudność w uzyskiwaniu wiarygodnego materiału do badań oraz stosunkowo niska czułość klasycznych metod diagnostycznych. Amplifikacja bakteryjnego DNA techniką PCR jest badaniem, które może przyczynić się do zwiększenia efektywności i czułości badań bakteriologicznych....
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Badania skuteczności ochrony katodowej nóg platformy wydobywczej
PublicationWykonano badania potencjałowe parametrów ochrony platformy wydobywczej Baltic Beta. Stwierdzono występowanie pełnej ochrony katodowej w rejonie dna. Stan techniczny anod wykazuje znaczne zużycie, jednakże w dalszym ciągu realizują proces ochorny
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Detection of Closing Crack in Beam Based on Responses Induced by Harmonic Excitation
PublicationThe non-linear contact model was chosen to simulate the closed crack in the cantilever beam. The study examines the shape and characteristics of the phase diagram of a cantilever beam with closed cracks. It investigates how various crack properties influence the geometry of the phase diagram and proposes a method for identifying cracks based on their features. The area of each closed curve in the phase diagram is determined using...
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Debonding Detection in Reinforced Concrete Beams with the Use of Guided Wave Propagation
PublicationOne of the most frequent damage of the reinforced concrete structures is debonding between steel bar and concrete cover. In the case of debonding occurrence not only the strength of the structure decreases, but also it is more vulnerable to corrosion damages. For this reason fast and effective methods of debonding detection in an early stage of its development need a significant boost. The paper presents analytical and experimental...
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Microscale diamond protection for a ZnO coated fiber optic sensor
PublicationFiber optic sensors are widely used in environmental, biological and chemical sensing. Due to the demanding environmental conditions in which they can be used, there is a risk of damaging the sensor measurement head placed in the measuring field. Sensors using nanolayers deposited upon the fiber structure are particularly vulnerable to damage. A thin film placed on the surface of the fiber end-face can be prone to mechanical damage...
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New, fast and cheap prediction tests for BRCA1 gene mutations identification in clinical samples.
PublicationDespite significant progress in cancer therapy, cancer is still the second cause of mortality in the world. The necessity to make quick therapeutic decisions forces the development of procedures allowing to obtain a reliable result in a quick and unambiguous manner. Currently, detecting predictive mutations, including BRCA1, is the basis for effectively treating advanced breast cancer. Here, we present new insight on gene mutation...
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Thermal Imaging Aided Assessment of a State of Equipment Under Test and its Protecting Elements
PublicationIn the paper the investigation results using thermal imaging methods are presented. The examined is a state of equipment under test and its protecting components. The estimations are done for the chosen protecting elements during surge immunity testing. The results show that the thermal imaging methods are useful for early detection of possible damage of a device being tested
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Szczepionki przeciw toksoplazmozie - aktualny stan badań
PublicationArtykuł prezentuje współczesną wiedzę na temat opracowywania skutecznej szczepionki przeciwko toksoplazmozie. W publikacji zebrane zostały informacje dotyczące nowych strategii w konstruowaniu efektywnych szczepionek opartych na antygenach natywnych, rekombinantowych, DNA lub RNA pasożyta.
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The influence of fresh cabbage and sauerkraut juices on the activity of protective enzymes in human cell lines
PublicationMechanizmami przeciwrakotwórczego działania związków zawartych w kapuście jest m.in. metaboliczna aktywacja enzymów II fazy detoksykacji, indukcja enzymów naprawczych DNA oraz aktywnośc przeciwutleniająca. Do enzymów II fazy odtruwania należą m.in. S-transferazy glutationowe (GST) oraz oksydoreduktaza chinonowa NQO1 zwana też DT-diaforazą. GST katalizują reakcję sprzęgania glutationu z wieloma różnymi typami ksenobiotyków, ułatwiając...
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Influence of IED Explosion on Lateral Dynamics of a Vehicle
PublicationThe paper describes field test results of heavy vehicle tire blow-outs. Both civilian and military vehicles are subjected to sudden loss of inflation pressure in one or more wheels, often accompanied by serious tire damage. Selected results related to the lateral dynamic of vehicle during and just after explosive tire destruction are reported
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Interactions of dissolved dsDNA with intercalating drug by anodic voltammetry and spectroscopy. Influence of pH
PublicationOddziaływanie związku przeciwnowotworowego C-1305 z DNA było badane przy pomocy impulsowej voltametrii różnicowej oraz spektroskopii UV/Vis. Triazoloakrydon C-1305 oddziałuje z DNA na dwóch drogach: przez interkalacje pomiedzy pary zasad oraz przez wiązanie sie w małym rowku helisy. W fizjologicznym pH (7,4) stałe wiązania wynoszą: K1=3,36E+5 i K2=0,18E+5 [1/M] a wielkość miejsca wiązania: n1=2,5 i n2=4,0, odpowiednio dla pierwszego...
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The use of a one-step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets
PublicationIntroduction: Dermatophytes are a closely related group of keratinophilic fungi. They encompass important etiological agents of superficial fungal infections. These fungi are able to invade keratinized tissues of humans and animals, causing dermatophytosis (ringworm) of hair, nails or skin. The aim: Traditional diagnostics of ringworm is based on morphological identification of cultured fungi and is time-consuming. Materials and methods:...
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Wave Method for Structural Health Monitoring: Testing Using Full-Scale Shake Table Experiment Data
PublicationAn algorithm of the wave method for structural health monitoring (SHM) is tested and calibrated using shake table experiment data of a full-scale, seven-story, reinforced-concrete building slice. The method is based on monitoring changes in the velocity of waves propagating vertically through the structure, identified by least-squares (LSQ) fit of beam models. The experiment was conducted by a team from the University of California,...
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Failure Monitoring and Condition Assessment of Steel-Concrete Adhesive Connection Using Ultrasonic Waves
PublicationAdhesive bonding is increasingly being incorporated into civil engineering applications. Recently, the use of structural adhesives in steel-concrete composite systems is of particular interest. The aim of the study is an experimental investigation of the damage assessment of the connection between steel and concrete during mechanical degradation. Nine specimens consisted of a concrete cube and two adhesively bonded steel plates...
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Experimental and theoretical study on high-temperature creep of VT6 titanium alloy under multi-axial loading conditions
PublicationIn the framework of damage mechanics, we discuss a new mathematical model that describes the kinetics of the stress–strain state and damage accumulation during material degradation by the mechanism of long-term strength under complex multiaxial stress state. An experimental and theoretical technique is proposed for determination of material parameters and scalar constitutive functions for damaged media based on specially set experiments...
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Baseline-free debonding detection in reinforced concrete structures by elastic wave propagation
PublicationThe article presents the results of the numerical and experimental analysis concerning wave propagation in reinforced concrete (RC) beams with various extent of debonding between the steel rod and concrete cover. The main aim of the paper was to consider the unsolved research gaps, which considerably limit the application of wave-based methods in practice. The propagation of the flexural wave modes excited and registered on the...
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Comparison of safety levels required by adequate regulations for cargo ships of different types
PublicationDuring the currently ongoing development of methods for assessing the safety of ships, it is imperative that a compromise between the accuracy of the results and the accessibility of obtaining results is reached. This paper shows the calculations of a theoretical hull against damage stability regulations as included in SOLAS 2020, ICLL 66/88 as amended in 2003 and MARPOL 78. The rules included in the currently valid regulations require...
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Eulerian motion magnification applied to structural health monitoring of wind turbines
PublicationSeveral types of defects may occur in wind turbines, as physical damage of blades or gearbox malfunction. A wind farm monitoring and damage prediction system is built to observe abnormal vibrations of elements of wind turbine: blades, nacelle, and tower. Contactless methods are developed which do not require turbine stopping. In this work, structural health monitoring of a wind turbine is evaluated using a conversion from the captured...
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Simulations of air and water flow in a model dike during overflow experiments
PublicationFlow in flood dikes, earth dams, and embankments occurs in variably saturated conditions, with pores of the earth material filled partly with water and partly with air. In routine engineering analysis, the influence of pore air is neglected and the air pressure is assumed equal to atmospheric. In some circumstances, for example, during overtopping of the dike by water, the effect of pore air on water flow and stability of the structure...
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Modelling reinforced concrete beams under mixed shear-tension failure with different continuous FE approaches
PublicationThe paper presents quasi-static numerical simulations of the behaviour of short reinforced concrete beams without shear reinforcement under mixed shear-tension failure using the FEM and four various constitutive continuum models for concrete. First, an isotropic elasto-plastic model with a Drucker-Prager criterion defined in compression and with a Rankine criterion defined in tension was used. Next, an anisotropic smeared crack...
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A grey box model of glucose fermentation and syntrophic oxidation in microbial fuel cells
PublicationIn this work, the fermentative and oxidative processes taking place in a microbial fuel cell (MFC) fed with glucose were studied and modeled. The model accounting for the bioelectrochemical processes was based on ordinary, Monod-type differential equations. The model parameters were estimated using experimental results obtained from three H-type MFCs operated at open or closed circuits and fed with glucose or ethanol. The experimental...
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Enhanced Electrochemical Performance of MnCo1.5Fe0.5O4Spinel for Oxygen Evolution Reaction through Heat Treatment
PublicationMnCo1.5Fe0.5O4 spinel oxide was synthesized using the sol−gel technique, followed by heat treatment at various temperatures (400, 600, 800, and 1000 °C). The prepared materials were examined as anode electrocatalysts for watersplitting systems in alkaline environments. Solid-state characterization methods, such as powder X-ray diffraction and X-ray absorption spectroscopy (XAS), were used to analyze the materials’ crystallographic...