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Search results for: HUMAN-RELATED BACTERIA
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BACTERIAL INACTIVATION VIA LASER-DRIVEN GOLD NANOPARTICLE HEATING: SIMULATION AND ANALYSIS
PublicationThis study utilizes CFD technique to simulate the inactivation of E. coli bacteria within a microfluidic chamber, employing gold nanoparticles irradiated by a laser beam. Employing a single-phase model, the presence of bacteria is considered by treating thermal properties in the governing equations as effective, combining those of water and bacteria using established correlations from scientific literature. The conversion of light...
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Zarządzanie wiedzą w małych przedsiębiorstwach oferujących wiedzochłonne usługi biznesowe
PublicationCelem artykułu jest przedstawienie praktyk zarządzania wiedzą oraz czynników wpływających na sukces, bądź porażkę w ich wdrażaniu na podstawie badania jakościowego przeprowadzonego w trzech firmach działających na terenie województwa pomorskiego. Firmy te należą do sektora małych i średnich przedsiębiorstw oraz oferują wiedzochłonne usługi biznesowe (knowledge-intensive business services – KIBS). W artykule podjęto próbę odpowiedzi...
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Wyzwania współpracy z robotem jako członkiem zespołu
Publication– Cel: Ocena, jakie są przekonania dotyczące współpracy z robotami w zespole, jakie są obawy i oczekiwania związane z „zatrudnianiem” robotów. – Metodyka badań: Badania ilościowe zrealizowane z zastosowaniem kwestionariusza ankiety w grupie 593 specjalistów IT. – Wyniki badań: Roboty nadal traktowane są głównie jako narzędzia, nie jako podmioty uczestniczące w pracy zespołu. Przekonanie, że obecność robota w zespole nie wyeliminuje...
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The influence of moderate pressure and subzero temperature on the shelf life of minced cod, salmon, pork and beef meat
PublicationThe effect of moderate pressure at subzero temperature on natural microflora of minced cod, salmony, pork and beef meat was studied. Pressure of 193 MPa at –20 °C caused reduction of total bacterial count in pork and beef meat by 1.1 and 0.6 log cycles, respectively, and by about 1.5 log cycles in fish meat. Under these conditions the psychrophilic and psychrotophic bacteria were below the detection limit (<10CFU/g of sample) in...
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Suppressors of lapC mutation identify new regulators of LpxC, which mediates the first committed step in lipopolysaccharide biosynthesis
PublicationGram-negative bacteria, such as Escherichia coli, are characterized by an asymmetric outer membrane (OM) with lipopolysaccharide (LPS) located in the outer leaflet and phospholipids facing the inner leaflet. E. coli recruits LPS assembly proteins LapB, LapC and LapD in concert with FtsH protease to ensure a balanced biosynthesis of LPS and phospholipids. We recently reported that bacteria either lacking the periplasmic domain of...
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DIAMOND AND RELATED MATERIALS
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Release of potassium from actvated sludge as a resultof use peroxyacetic acid = Uwalnianie potasu z osadu czynnego pod wpływem kwasu nadoctowego
PublicationThe phenomenon of potassium efflux (GGKE - glutathione-gated potassium efflux) from the calls of activated sludge bacteria in the result of peroxyacetic acid (CH3CO3H) dosing was investigated. Addition of the strong oxidant to the activated sludge environment caused activation of the defense mechanisms of bacterial cells, related to glutathione transformations, which resulted in the significant increase of potassium ions concentration....
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Lipidomics of Staphylococcus aureus – a new insight into the antibiotic resistant phenotype
PublicationStaphylococcus aureus is an aggressive pathogen responsible for a variety of diseases, in cluding life-threatening sepsis. Many strains of these bacteria are resistant to multiple classes of antibiotics, which is a substantial clinical problem in the treatment of S. aureus infections. We developed a comprehensive untargeted lipidomic workflow, including sample preparation, liquid chromatography-quadrupole-time-of-flight mass spectrometry...
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High prevalence of Escherichia coli belonging to the B2+D phylogenetic group in inflammatory bowel disease
PublicationBACKGROUND: It is not clear which species of bacteria may be involved in inflammatory bowel disease (IBD). One way of determining which bacteria might be likely candidates is to use culture-independent methods to identify microorganisms that are present in diseased tissues but not in controls. AIMS: (1) To assess the diversity of microbial communities of biopsy tissue using culture-independent methods; (2) to culture the bacteria...
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REVIEW ON COMPETITION АОB AND NОВ AS AN IMPORTANT ELEMENT FOR COST EFFECTIVE METHODS OF REMOVING NITROGEN FROM WASTEWATER
PublicationThe aim of this paper is analysis of process principles and possible factors used to induce inhibition of nitrite oxidation by NOB (Nitrite Oxidizing Bacteria) which could be obtained during not very well understood processes: so called “shortcut” or “partial” nitrification as well deammonification, by qualitative/quantitative studies of AOB (Ammonium Oxidizing Bacteria) and NOB populations in activated...
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Ribes nigrum L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles
PublicationSilver nanoparticles (Ag NPs) represent one of the most widely employed metal-based engineered nanomaterials with a broad range of applications in different areas of science. Plant extracts (PEs) serve as green reducing and coating agents and can be exploited for the generation of Ag NPs. In this study, the phytochemical composition of ethanolic extract of black currant (Ribes nigrum) leaves was determined. The main components...
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Inhibition effect of free ammonia on deammonification process under different intermittent aeration strategies in sequencing batch reactor
PublicationThe deammonification process involves two steps. The first step is the partial nitrification of ammonia (NH4-N) by ammonia oxidizing bacteria (AOB) to produce nitrite (NO2-N) and the second step is the anammox process to “anaerobically” oxidize ammonia to nitrogen gas with nitrite as an electron acceptor (Lackner et al., 2014). Free ammonia (FA) plays a significant role in the stable, long-term deammonification system operation....
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Synthetic lipopeptides as potential topical therapeutics in wound and skin care: in vitro studies of permeation and skin cells behaviour
PublicationSeveral Gly-His-Lys analogues were obtained to investigate their antimicrobial properties. Three lipophilic analogues with the structure numbering 1b, 2b, 4b, exhibit significant effect against bacteria and were selected for in vitro evaluation of skin cells behaviour. In the present studies, an in vitro model of wound repair, proliferative cell staining, and tracking of living cells were used. Cell proliferation and cell migration,...
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Pharmacomicrobiomics of cell-cycle specific anti-cancer drugs – is it a new perspective for personalized treatment of cancer patients?
PublicationIntestinal bacteria are equipped with an enzyme apparatus that is involved in the active biotrans-formation of xenobiotics, including drugs. Pharmacomicrobiomics, a new area of pharmacology, analyses interactions between bacteria and xenobiotics. However, there is another side to the coin. Pharmacotherapeutic agents can significantly modify the microbiota, which consequently affects their efficacy. In this review, we comprehensively...
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Gravity-related research (2020/21)
e-Learning CoursesThe goal of the subject is to extend of the knowledge gained in the framework of general mechanics (statics, kinematics, dynamics). Students will be familiarized with the description of the kinematics and dynamics of motion in typical gravity-related research conditions (microgravity in parabolic flights, drop towers, sounding rockets) as well as gravity-related research platforms, its opportunities and limitations. Additionally,...
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Assessing ecotoxicity, synergic and antagonistic actions of commonly used components of polymerization mixture in molecularly imprinted polymers preparation process
PublicationIncreasingly, the challenge is the optimal process of developing a suitable MIP-type sorptive material (taking into account its final form and application area), which is mainly related to the efficiency/effectiveness of the polymerization process. The lack of reaction or its low efficiency generates additional waste and puts it into the environmental cycle. The main aim of the performed research was the estimation of ecotoxicity,...
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Soil and groundwater fecal contamination as a result of sewage sludge land application.
PublicationThe release of sludge-born bacteria and their further transport in subsurface was studied. The migration of bacteria was investigated in column experiments which were carried out under the conditions corresponding to naturally occurring extreme rainfall. Coal fly ash as well as coarse and medium grained sand, which properties are similar to the soils present in degraded areas, were used as column beds. Sewage sludge was applied...
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Cloning, expression, purification and characterization of recombinant trehalose synthase from Deinococcus radiodurans.
PublicationTrehalose (α-D-glucopyranosyl-1,1-α-D-glucopyranoside) is a nonreducing disacharide in which the two glucose molecules are linked trough a α-1,1-glycosidic bond. Trehalose is readily hydrolyzed to glucose and can be used as a reserve of that sugar in the cell. The presence of trehalose was found in the cells of fungi and yeasts, bacteria, nematodes, insects, eggs, pupae and some plants. The characteristics of trehalose make it...
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IMPACT OF DIRECT CURRENT ON NITRIFYING BIOMASS
PublicationEffects of short term exposure of nitrifying biomass to direct current were studied. DC was applied to mixed liquor from municipal BNR plant using graphite electrodes in no substrate limiting conditions. Both, ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) populations were affected by the current, however in opposite ways. The AOB population activity was inhibited by applied voltage. Activity decrease was...
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Isolation and properties of recombinant trehalose synthase
PublicationBiosynthesis of trehalose is carried out via various matabolic pathways, including the use of trehalose synthase. Bacteria of the genus Deionococcus are microorganisms which produce trehalose synthase catalizing conversion of maltose into trehalose. In this work recombinant trehalose synthase from D.radiodurans was characterized.
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Antibiotic resistance in wastewater, does the context matter? Poland and Portugal as a case study
PublicationAntibiotic resistance has been considered a major human health threat that may endanger the success of medicine. Recent studies have unveiled worldwide asymmetries of antibiotic resistance occurrence, being factors as diverse as climate, socioeconomic, or antibiotic use possible drivers of such asymmetric distribution. In Europe, where clinical antibiotic resistance is surveyed for more than 20 years, the European Center for Disease...
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Evaluation of antimicrobial activities of powdered cuttlebone against Klebsiella oxytoca, Staphylococcus aureus, and Aspergillus flavus
PublicationBackground: The presence of medicines in the environment is considered as a serious threat to the human health. The entrance of these substances into the water sources causes soil pollution, which eventually leads to the environmental pollution and it creates some problems for the public health. Also, increasing antibiotic resistant bacteria has attracted the attention of researchers to the use of natural resources such as marine...
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Potential of Silver Nanoparticles in Overcoming the Intrinsic Resistance of Pseudomonas aeruginosa to Secondary Metabolites from Carnivorous Plants
PublicationCarnivorous plants are exemplary natural sources of secondary metabolites with biological activity. However, the therapeutic antimicrobial potential of these compounds is limited due to intrinsic resistance of selected bacterial pathogens, among which Pseudomonas aeruginosa represents an extreme example. The objective of the study was to overcome the intrinsic resistance of P. aeruginosa by combining silver nanoparticles (AgNPs)...
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Probiotic potential of Bacillus Isolates from Polish Bee Pollen and Bee Bread
PublicationThe main goal of this study was the evaluation of the probiotic potential of 10 Bacillus spp. strains isolated from 5 bee bread and 3 bee pollen samples. The antagonistic interaction with Staphylococcus aureus and Escherichia coli was a primary criterion for the preliminary selection of the isolates. Three out of ten strains—PY2.3 (isolated from pollen), BP20.15 and BB10.1 (both isolated from bee bread)—were found to be possible...
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A shear stress micromodel of urinary tract infection by the Escherichia coli producing Dr adhesin
PublicationIn this study, we established a dynamic micromodel of urinary tract infection to analyze the impact of UT-segment-specific urinary outflow on the persistence of E. coli colonization. We found that the adherence of Dr+ E. coli to bladder T24 transitional cells and type IV collagen is maximal at lowest shear stress and is reduced by any increase in flow velocity. The analyzed adherence was effective in the whole spectrum of physiological...
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Toxicity and chemical analyses of airport runoff waters in Poland
PublicationThe aim of this study was to assess the ecotoxicological effects of various compounds in complex airport effluents using a chemical and ecotoxicological integrated strategy. The present work deals with the determination of sum of PCBs, PAHs, pesticides, cations, anions, phenols, anionic, cationic, non-ionic detergents, formaldehyde and metals – as well as TOC and conductivity – in runoff water samples collected from 2009 to 2011...
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Preliminary Investigation on Rapid Potassium Efflux from Activated Sludge in Response to Use of Peroxyacetic Acid
PublicationPreliminary research on activated sludge potassium efflux (GGKE - glutathione-gated potassium efflux) as a result of peroxyacetic acid (PAA) dosing were performed. Similarily as in case of chlorine dosing the PAA dosing causes initiation of bacteria defense mechanisms, related to the transformations of glutathione tripeptide, resulting in the increase of potassium ions concentration in the activated sludge environment. In the range...
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Influence of Storage Time and Temperature on the Toxicity, Endocrine Potential, and Migration of Epoxy Resin Precursors in Extracts of Food Packaging Materials
PublicationThe aim of the present study was to establish a standard methodology for the extraction of epoxy resin precursors from several types of food packages (cans, multi-layered composite material, and cups) with selected simulation media (distilled water, 5% ethanol, 3% dimethyl sulfoxide, 5% acetic acid, artificial saliva) at different extraction times and temperatures (factors). Biological analyses were conducted to determine the acute...
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Kobiety i mezczyźni w zespole - wartość czy wyzwanie
PublicationArtykuł dotyczy zarządzania zespołami zróżnicowanymi pod względem płci. Zaprezentowano wyniki badań przeprowadzonych wśród słuchaczy podyplomowych studiów Zarządzania Zasobami Ludzkimi. Celem badań była weryfikacja sposobu postrzegania zespołów zróżnicowanych pod względem płci przez osoby związane z zarządzaniem kapitałem ludzkim. Dodatkowo oceniono poziom wykorzystywania wartości takich zespołów w firmach reprezentowanych przez...
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Comparison of disk and tube methods for estimation of biostatic properties of polyurethanes based on synthetic poly[(R,S)-3-hydroxybutyrate
PublicationThe influence of novel aliphatic polyurethanes on growth of Staphyloccocus aureus (SA)in the around of polymer samples has been estimated by disk method. It was staed that the growth of SA was inhibited.The influence of polyurethanes on planctonic Staphyloccocus aureus was also pefrormed by tube method. In this test the slight decrease of bacteria concentration was noticed.
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WYKORZYSTANIE SIECI NEURONOWYCH DO SYNTEZY MOWY WYRAŻAJĄCEJ EMOCJE
PublicationW niniejszym artykule przedstawiono analizę rozwiązań do rozpoznawania emocji opartych na mowie i możliwości ich wykorzystania w syntezie mowy z emocjami, wykorzystując do tego celu sieci neuronowe. Przedstawiono aktualne rozwiązania dotyczące rozpoznawania emocji w mowie i metod syntezy mowy za pomocą sieci neuronowych. Obecnie obserwuje się znaczny wzrost zainteresowania i wykorzystania uczenia głębokiego w aplikacjach związanych...
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Chracteristics of anaerobic ammonium oxidation initated by anaerobic granular sludge
PublicationTwo different mixed sludge, anaerobic granular sludge(R1) and anaerobic granular sludge with mature anaerobic ammonium oxidation (ANAMMOX) sludge (R2 )were used as the inocula in two up-flow reactors to enrich anaerobic ammonia-oxidizing bacteria (AnAOB),respectively.The total nitrogen removal rate of R1 reached 74% in 99 days, while R2 remained above 70% in only 48 days.The content of extracellular polymeric substance (EPS) in...
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Direct determination of cadaverine in the volatile fraction of aerobically stored chicken breast samples
PublicationTo supplement the currently used methods for poultry meat shelf life assessment, it might be necessary to develop a technique for rapid headspace analysis of volatiles with no prior sample preparation step. Biogenic amines, in particular cadaverine, are considered meat spoilage indicators. Described in this article are the results of a preliminary investigation of the applicability of proton transfer reaction mass spectrometry...
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Polymer biodegradable coatings as active substance release systems for urological applications
PublicationCatheter-associated urinary tract infections are the result of catheterization of the bladder. The risk of infection is directly proportional to the length of the bladder catheterization. Bacteria cells have the ability to adhere and create the biofilm on the surface of catheter materials. Bacteria’s biofilm is an extremely beneficial environment of existence for microorganisms. Microorganisms that are an integral part of the biofilm...
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Evidence of mutations conferring resistance to clarithromycin in wastewater and activated sludge
PublicationThe occurrence of clarithromycin in wastewater samples and of the activated sludge bacteria possibly resistant to this pharmaceutical was the object of the study. Samples of wastewater or activated sludge were taken from a municipal wastewater treatment plant in summer and winter and characterised regarding their clarithromycin concentrations and the presence of nucleic acid fragments (Cla-sequences) known to be responsible for...
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The Chemical and Biological Properties of Nanohydroxyapatite Coatings with Antibacterial Nanometals, Obtained in the Electrophoretic Process on the Ti13Zr13Nb Alloy
PublicationThe risk of an early inflammation after implantation surgery of titanium implants has caused the development of different antimicrobial measures. The present research is aimed at characterizing the effects of nanosilver and nanocopper dispersed in the nanohydroxyapatite coatings, deposited on the Ti13Zr13Nb alloy, and on the chemical and biological properties of the coatings.The one-stage deposition process was performed by the...
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Microbiological Survey in Two Arctic Fjords: Total Bacterial Number and Biomass Comparison of Hornsund and Kongsfjorden
PublicationTwo microbiological parameters: total bacterial number (TBN) and biomass (BBM) were studied in two Arctic fjords: Hornsund and Kongsfjorden. Samples were collected from three sampling points in each fjord, from various water depth layers: from the surface to 75 m depth. Total bacterial number and biomass were examined using the DAPI staining and direct count method. The greater amount of bacteria, as well as highest bacterial biomass...
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Microbiological Survey in Two Arctic Fjords: Total Bacterial Number and Biomass Comparison of Hornsund and Kongsfjorden
PublicationTwo microbiological parameters: total bacterial number (TBN) and biomass (BBM) were studied in two Arctic fjords: Hornsund and Kongsfjorden. Samples were collected from three sampling points in each fjord, from various water depth layers: from the surface to 75 m depth. Total bacterial number and biomass were examined using the DAPI staining and direct count method. The greater amount of bacteria, as well as highest bacterial biomass...
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Comparison of the Efficiency of Deammonification under Different DO Concentrations in a Laboratory-Scale Sequencing Batch Reactor
PublicationThe efficiency of deammonification depends on the cooperation of ammonium oxidizing bacteria and archaea (AOB/AOA), anaerobic ammonium oxidizing bacteria (AnAOB) and the effective suppression of nitrite oxidizing bacteria (NOB) that compete with AnAOB for nitrite (NO2 -N). One of the effective NOB suppression strategies is intermittent aeration. However, it is important to have a good understanding of the optimum dissolved oxygen...
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Investigation of Microbes in the Stratosphere – Stardust Project
PublicationThe stratospheric microbiota has been investigated many times using the methods of classical microbiology. In this research a few modern methods, including NGS sequencing and multiple displacement amplification of DNA, were in use. The analysis of metagenome helped to determine the content of various species of bacteria in the sample collected in the stratosphere by a hydrogen-filled balloon holding...
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Biosurfactants - Biodegradability, toxicity, efficiency in comparison with synthetic surfactants
PublicationBiosurfactants are natural surface active agents produced by variety of bacteria yeast and fungi. A review of their biodegradability, toxicity and efficiency is presented in order to compare their properties with synthetic surfactants. Due to their high efficiency in many applications and environmentally friendly character we tried to apply them in oil refining process. The efficiency of biosurfactants in this application was very...
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The influence of biological environment on the appearance of silver-coated implants
PublicationDespite antibiotics preventive treatment before and after implantation, risk of infection is real. The infections at the implant surface developein a few months after applying them into the body. To prevent the development of bacteria and to reduce the risk of infection, implants coated with silver layer come into use. The paper presents the research results in biological environment on vein and orthopaedicimplants covered with...
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A Perspective on Missing Aspects in Ongoing Purification Research towards Melissa officinalis
PublicationMelissa officinalis L. is a medicinal plant used worldwide for ethno-medical purposes. Today, it is grown everywhere; while it is known to originate from Southern Europe, it is now found around the world, from North America to New Zealand. The biological properties of this medicinal plant are mainly related to its high content of phytochemical (bioactive) compounds, such as flavonoids, polyphenolic compounds, aldehydes, glycosides...
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Assessment of ecotoxicity and total volatile organic compound (TVOC) emissions from food and children's toy products
PublicationThe development of new methods for identifying a broad spectrum of analytes, as well as highly selective tools to provide the most accurate information regarding the processes and relationships in the world, has been an area of interest for researchers for many years. The information obtained with these tools provides valuable data to complement existing knowledge but, above all, to identify and determine previously unknown hazards. Recently,...
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Phage Therapy—Challenges, Opportunities and Future Prospects
PublicationThe increasing drug resistance of bacteria to commonly used antibiotics creates the need to search for and develop alternative forms of treatment. Phage therapy fits this trend perfectly. Phages that selectively infect and kill bacteria are often the only life-saving therapeutic option. Full legalization of this treatment method could help solve the problem of multidrug-resistant infectious diseases on a global scale. The aim of...
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Enhancement of photocatalytic-based processes by mono- and bimetallic (CuPd) rutile loaded nanoparticles for antibiotic resistance genes and facultative pathogenic bacteria removal
PublicationThe aim of the study was the strong reduction of facultative pathogenic bacteria (FPB), and clinically relevant antibiotic resistance genes (ARGs) from secondary effluent. To evaluate the ARGs removal efficiency comparative study of individual unit processes and combined AOPs has been performed. The present work investigated: i) removal of selected ARGs, namely blaTEM, ermB, qnrS, tetM, five FPB as well as 16S rDNA and the integrase...
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Paenibacillus alvei MP1 as a Producer of the Proteinaceous Compound with Activity against Important Human Pathogens, Including Staphylococcus aureus and Listeria monocytogenes
PublicationAn emerging need for new classes of antibiotics is, on the one hand, evident as antimicrobial resistance continues to rise. On the other hand, the awareness of the pros and cons of chemically synthesized compounds’ extensive use leads to a search for new metabolites in already known reservoirs. Previous research showed that Paenibacillus strain (P. alvei MP1) recovered from a buckwheat honey sample presented a wide spectrum of...
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Robotics for human health and performance 03.2022
e-Learning CoursesThis course is to provide knowledge in area of biomechanics, necessary to design instrumentation for human health and performance, and automatics necessary to design simple instrumentation as well as about human-robot interface.
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Rearrangements of the nitrifiers population in an activated sludge system under decreasing solids retention times
PublicationDue to the key role of nitrite in novel nitrogen removal systems, nitrite oxidizing bacteria (NOB) have been receiving increasing attention. In this study, the coexistence and interactions of nitrifying bacteria were explored at decreasing solids retention times (SRTs). Four 5-week washout experiments were carried out in laboratory-scale (V=10 L) sequencing batch reactors (SBRs) with mixed liquor from two full-scale activated sludge...
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Silver-modified titania with enhanced photocatalytic and antimicrobial properties under UV and visible light irradiation
PublicationCommercial titania photocatalysts were modified with 2 wt% of silver by photodeposition. The properties of the samples were characterized by DRS, XPS, XRD, FE-SEM and STEM. The modified samples exhibited activity under visible light and enhanced activity under UV irradiation for 2-propanol and acetic acid oxidation, respectively. The time-resolved microwave conductivity (TRMC) analysis indicated that enhanced activity (2.5–8-fold...