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Identification of anisakid nematodes from the Southern Baltic Sea usingPCR-based methods
PublicationZaproponowano metodę identyfikacji nicieni gatunków Anisakis, Hysterothyla-cium, Contracaecum przy użyciu techniki PCR/RFLP.
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Comparative studies of the Acinetobacter genus and the species identification method based on the recA sequences
PublicationZaproponowano metodę genotypowania bakterii rodzaju Acinetobacter, opartą o analizę sekwencji genów recA, amplifikację fragmentów genu recA i analizę profili fragmentów restrykcyjnych po cięciu produktów reakcji PCR enzymami restrykcyjnymi (recA PCR/RFLP). Profile RFLP otrzymane za pomocą enzymu Tsp509I są użyteczne w identyfikacji poszczególnych gatunków bakterii rodzaju Acinetobacter. Na podstawie analizy otrzymanych sekwencji...
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Modeling lignin extraction with ionic liquids using machine learning approach
PublicationLignin, next to cellulose, is the second most common natural biopolymer on Earth, containing a third of the organic carbon in the biosphere. For many years, lignin was perceived as waste when obtaining cellulose and hemicellulose and used as a biofuel for the production of bioenergy. However, recently, lignin has been considered a renewable raw material for the production of chemicals and materials to replace petrochemical resources....
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Heat Dissipation from the Power Cable in the Casing Pipe
PublicationThe current carrying capacity of a power cable determines its ability to carry a specific current and is related to the efficiency of dissipation of heat generated in the cable laid in a specific environment. The studies were conducted in order to determine how the characteristics of the medium around the cable affected its temperature, and thus the ability of the cable to transmit electricity. The experimentation in the lab and...
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Forming Ni-Fe and Co-Fe Bimetallic Structures on SrTiO3-Based SOFC Anode Candidates
PublicationThe aim of this work was to verify the possibility of forming Ni-Fe and Co-Fe alloys via topotactic ion exchange exsolution in Fe-infiltrated (La,Sr,Ce)0.9(Ni,Ti)O3-δ or (La,Sr,Ce)0.9(Co,Ti)O3-δ ceramics. For this purpose, samples were synthesized using the Pechini method and then infiltrated with an iron nitrate solution. The reduction process in dry H2 forced the topotactic ion exchange exsolution, leading to the formation of...
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Quantitative Risk Assessment in Construction Disputes Based on Machine Learning Tools
PublicationA high monetary value of the construction projects is one of the reasons of frequent disputes between a general contractor (GC) and a client. A construction site is a unique, one-time, and single-product factory with many parties involved and dependent on each other. The organizational dependencies and their complexity make any fault or mistake propagate and influence the final result (delays, cost overruns). The constant will...
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Nondestructive corrosion degradation assessment based on asymmetry of guided wave propagation field
PublicationThe article presents the results of numerical and experimental investigation of guided wave propagation in steel plates subjected to corrosion degradation. The development of novel procedures allowing for the assessment of the corrosion degradation level is crucial in the effective diagnostics of offshore and ship structures that are especially subjected to aggressive environments. The study’s main aim is to investigate the influence...
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The Influence of Global Corrosion Degradation on Localized Damage Detection Using Guided Waves
PublicationThis paper presents the results of a numerical analysis of the influence of corrosion degradation of metal plates on the wave propagation phenomenon. There are several different corrosion types, but general and pitting corrosion are the most common. General corrosion is more or less uniformly distributed over the entire exposed surface of the metal while pitting corrosion takes the form of localized cracks. Because the general...
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Corrosion degradation monitoring of ship stiffened plates using guided wave phase velocity and constrained convex optimization method
PublicationThe study presents an experimental investigation on the corrosion degradation level assessment using nondestructive wave-based methods. The degradation level of ship structural elements has been assessed in two different ways. The first one is based on the spectral decomposition and zero-crossing incorporated reconstruction of the dispersion curve approach of the antisymmetric Lamb wave mode and the best matching of the theoretical...
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A new assay based on terminal restriction fragment length polymorphism of homocitrate synthase gene fragments for Candida species identification
PublicationCandida sp. have been responsible for an increasing number of infections, especially in patients with immunodeficiency. Species specific differentiation of the Candida sp. is difficult in routine diagnosis. This identification can have a highly significant association in therapy and prophylaxis. This work has shown a new application of terminal restriction fragment length polymorphism (t-RFLP) method in molecular identification...
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Building Information Modelling as an opportunity and risk for stakeholders involved in construction investment process
PublicationThe requirements to apply Building Information Modelling (BIM) in public investments worldwide are currently very high. Significant interest (sometimes formulated also as a requirement) in BIM technology can be observed also among private investors. Design technology that applies BIM is supported by many private investors due to its numerous advantages. A growing group of construction designers (steel, concrete and reinforced concrete...
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Modyfikacje polimeraz DNA jako rozwiązania problemów w reakcjach PCR
PublicationReakcja PCR już od wielu lat jest podstawowym narzędziem stosowanym w biotechnologii molekularnej. Nadal napotykamy jednak liczne problemy podczas jej przeprowadzania związane z trudnymi matrycami, obecnością inhibitorów itp. Optymalizacja reakcji PCR jest więc niezbędna w celu poprawy wyników. Jednym z rozwiązań tych problemów może być modyfikowanie polimeraz DNA. W powyższym artykule opisano problemy i rozwiązania zależne polimerazy...
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The influence of historical conditions on time and cost of construction project
PublicationConducting construction works in a building or in an area entered in the historic register is associated with the contracting authority's risk of incurring higher than planned costs of works, as well as delays in their implementation. In many cases, these deviations are completely independent of contracting authority and contractor. The aim of this article is to indicate the reasons for cost and time changes in the construction...
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Risk of Cost Overruns in Implementation of Building Investment in Urban Conditions in the Aspect of Historical Background of its Location
PublicationThe article is a continuation of the authors' analyzes on the risk of delays in implementation of building investment in urban conditions in the aspect of historical background of its location. In case of construction investment located in the historic part of the city, the possible accumulation of many unfavourable factors, which in course of its implementation, may constitute a serious source of cost overruns and disruptions,...
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The analysis of the influence of the corrosion protection method of selected steel elements on the steel structure life cycle costs
PublicationSteel products are widely used in the construction for the performance of essential and additional elements of engineering structures. They are characterized by high tensile strength, compression and bending, the structure homogeneity, the possibility of assembly regardless of the season and climatic conditions, ease of processing and the possibility of almost any shape. The main disadvantage of steel structures is their high susceptibility...
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Risk of Delays in Implementation of Building Investment in Urban Conditions in the Aspect of Historical Background of its Location
PublicationThe implementation of a construction investment in urban conditions is extremely complex, and at the same time exposed to a high risk of a specific character. In case of construction investment located in the historic part of the city, the possible accumulation of many unfavourable factors, which in course of its implementation, may constitute a serious source of delays and disruptions, should be taken into account at the preparation...
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Application of Game Theory to Conflict Management in a Construction Contract
PublicationInterest has recently grown in the application of game theory (GT) to solve a number of diverse problems in the field of construction. The use of GT by a general contractor (GC) of construction works to indicate the best strategy leading to winning court proceedings in a situation of conflict with investor (IN), has not been investigated until now. Thus the aim of this paper is to indicate the optimal strategy from the GC viewpoint...
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Effect of osmolytes on the thermal stability of proteins: replica exchange simulations of Trp-cage in urea and betaine solutions
PublicationAlthough osmolytes are known to modulate the folding equilibrium, the molecular mechanism of their effect on thermal denaturation of proteins is still poorly understood. Here, we simulated the thermal denaturation of a small model protein (Trp-cage) in the presence of denaturing (urea) and stabilizing (betaine) osmolytes, using the all-atom replica exchange molecular dynamics simulations. We found that urea destabilizes Trp-cage...
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Wykorzystanie fourierowskiej spektroskopii w podczerwieni do badania stężenia gazów wylotowych z tlenkowego ogniwa paliwowego zasilanego biogazem
PublicationW dzisiejszych czasach bardzo ważne jest pozyskiwanie energii ze źródeł odnawialnych i bezpiecznych dla środowiska. Jedną z obiecujących technologii są tlenkowe ogniwa paliwowe (ang. Solid Oxide Fuel Cell, SOFC). Stanowią one bezpieczną i ekologiczną alternatywę dla energii uzyskanej z paliw kopalnych. SOFC charakteryzuje się sprawnością energetyczną na poziomie 45-60 %, podczas gdy sprawność klasycznych elektrowni cieplnych nie...
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Building Information Modeling in Small and Middle Sized Buildings – Case Study
PublicationBuilding Information Modeling (BIM) is a technology designed to improve and integrate all engineering aspects in the construction design process. Tools that incorporate the idea of BIM are becoming more advanced and also more popular. Available software solutions have already reached an assumable high level of development, but still are not as widespread and used on nearly as many construction projects as could be expected. Therefore...
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Criteria for evaluation and selection of the best offer for the Contract Engineer service
PublicationThe purpose of the legal regulations regarding public procurement in EU countries is to ensure effective funds' spending. When assessing and selecting the best offer, the contracting entities have at their disposal many different criteria, including non-price criteria. Their proper selection and application is necessary to ensure the high quality of the ordered product, delivery or service. Making an order for intellectual services,...
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The Multiplatform Environment for Simulation and Features Estimation of Mixed-Signal Devices
PublicationThe use of simulation laboratories is gaining popularity in thedomains of engineering programs. However, the experience in teaching showsthat the simulation itself is not very effective in didactic processes. Teachingprocesses in thefield of specialist subjects, designed for students of technicaluniversities, should be based on direct operations performed by the student onreal devices. At the same time, at the later stages of didactic...
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Wpływ metody trawienia i spiekania na właściwości mechaniczne powłok z nanorurek węglowych osadzonych elektroforetycznie na podłożu Ti13Nb13Zr
PublicationDo eksperymentu użyto stopu tytanu Ti13Zr13Nb, który ze względu na swój skład chemiczny i właściwości mechaniczne stanowi doskonały materiał do zastosowań w inżynierii medycznej. Celem pracy była ocena wpływu przygotowania podłoża (rodzaj zastosowanego odczynnika trawiącego) oraz obróbki końcowej (proces spiekania) powłok z wielościennych nanorurek węglowych na ich właściwości mechaniczne (nanotwardość, moduł Younga). Do wytworzenia...
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Cost Analysis of Prefabricated Elements of the Ordinary and Lightweight Concrete Walls in Residential Construction
PublicationGlobal economic growth causes an increase in natural resources exploitation, particularly in construction branch. The growing use of electricity contributes to climate change. Therefore, it is necessary to search the solutions, which will allow for reducing natural resources exploitation. One of the many opportunities to do that is the application of the recycled materials. The authors of the given article have analyzed three variants...
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Identification of volatile compounds in raw spirits of different organoleptic quality
PublicationGłównym celem prezentowanych badań było określenie składu lotnej frakcji destylatów rolniczych, a następnie wyodrębnienie profili zapachowych destylatów rolniczych różniących się właściwościami sensorycznymi ocenionymi na podstawie klasycznej analizy sensorycznej. Profile zapachowe tworzono z wykorzystaniem kapilarnej chromatografii gazowej połączonej ze spektrometrią mas (GC-MS). Do ekstrakcji lotnych związków zastosowano technikę...
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Fizyka jazdy na rowerze
PublicationOpisano podstawowe zjawiska fizyczne zachodzące podczas jazdy człowieka na rowerze. Zaprezentowano obecny stan wiedzy na temat czynników mających wpływ na stabilność układu człowiek-rower w trakcie ruchu w oparciu o analizy przeprowadzone przez niezależne grupy naukowców na całym świecie.
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Pharmaceutical applications of surfactants
PublicationIn this study we focused on biological activity of various surfactants. According to our research, they con act both as carriers as well as therapeutic agents. Depending on their concentration, they can create different structures in a solution, which influence their properties. Liposomes have a significant role in drug delivery system. Surfactants in from of bilayer are analogous to natural biological cell membrane.
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The New LM-PCR/Shifter Method for the Genotyping of Microorganism
PublicationTechniques relies on the ligation of appropriates adapters (LM-PCR) as AFLP, PCR MP and ADSRRS are successfully used for epidemiological studies for prokaryotic and eukaryotic microorganisms. In this study we propose a new method, called the LM-PCR/Shifter, based on the use of a Class IIS restriction enzyme giving restriction fragments with different 4 base 5' overhangs (Shifter) and the ligation of appropriate oligonucleotide...
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DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation
PublicationA new diagnostic kit for K. oxytoca specific differentiation based on ddLMS PCR (ang. double digest ligation Mediated PCR) technique is shown. As a species-specific DNA fragment pehX gene, encoding the enzyme polygalactouronase, was chosen. The genome sequence of K. oxytoca is digested with two endonucleases: AclI and BclI which cut DNA before and after pehX gene. The polymorphic DNA fragments are ligated with AclI-end-specific...
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A study on fibre-reinforced concrete elements properties based on the case of habitat modules in the underwater sills
PublicationHydrotechnical constructions are mostly objects functioning in extreme conditions and requiring a custom-made construction project. In the case of using prefabricated elements, it is required to develop production, transport, assembly, conservation and repair technology. Concerning the problem of concrete cracks, modern repair systems allow positive effects to be achieved in many cases of concrete elements repair. In this work...
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Compressive Sensing Approach to Harmonics Detection in the Ship Electrical Network
PublicationThe contribution of this paper is to show the opportunities for using the compressive sensing (CS) technique for detecting harmonics in a frequency sparse signal. The signal in a ship’s electrical network, polluted by harmonic distortions, can be modeled as a superposition of a small number of sinusoids and the discrete Fourier transform (DFT) basis forms its sparse domain. According to the theory of CS, a signal may be reconstructed...
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Evidence-Based Risk Management for Civil Engineering Projects Using Bayesian Belief Networks (BBN)
PublicationThe authors are seeking new methods for improving the efficiency of the investments associated with the maintenance and operation of existing civil engineering structures. It is demonstrated how the knowledge about the elements of construction and operation phases and their relationships, combined with monitoring data can be used for more effective management of the risks associated with civil engineering projects. The methodology...
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A Model for Risk Assessment and Management of Construction Projects in Urban Conditions
PublicationThe authors are seeking new methods for improving the efficiency of the investments associated with the maintenance and operation of existing civil engineering structures. It is demonstrated how the knowledge about the elements of construction and operation phases and their relationships, combined with monitoring data can be used for more effective management of the risks associated with civil engineering projects. The methodology...
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Evaluating the antibacterial activity of muramyl dipeptide derivatives, retro-tuftsin derivatives, and anthraquinone oligopeptides against a range of pathogenic bacteria
PublicationSearch for new and efficient antibiotic is crucial because of microbial drug resistance and problems with side effects of the administered medication. In this study, we evaluate the in vitro microbiological activity of muramyl dipeptide derivatives, retro-tuftsin derivatives (i.e., tuftsin with reversed amino acid sequences), and combinations of retro-tuftsin derivatives with substituted anthraquinones. The potency of the investigated...
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The Laboratory Stand for the Evaluation of Heat Dissipation from the Power Cable in a Casing Pipe
PublicationThe current carrying capacity of a power cable determines its efficiency, which depends on its capability to dissipate heat produced inside the cable, as a result of current flow, into the surrounding. The studies were conducted to determine how the characteristics of the medium around the cable affected its temperature and, as a result, its ability to transport electricity. The experimentation in the lab and real measurements...
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Experiment-Based Study of Heat Dissipation from the Power Cable in a Casing Pipe
PublicationThe paper deals with the important challenges in terms of electricity transmission by means of underground cable lines. The power cable’s performance is characterized by an ampacity that represents its maximum electric current-carrying capacity. The ampacity of power cables depends on their ability to diffuse the heat generated by the current flow into the environment. In the performed research, the analysis of the efficiency of...
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Experimental and numerical identification of corrosion degradation of ageing structural components
PublicationThe study presents experimental and numerical identification of corrosion degradation of thin-walled structural components employing guided wave propagation. The steel structural components are subjected to through-thickness varying corrosion degradation levels. The developed approach using the non-destructive guided wave-propagation quantifies the equivalent average corrosion degradation level by exploring a limited number of...
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Entropy Measures of heart rate variability for short ECG datasets in patients with congestive heart failure
PublicationWe investigated the usefulness of entropy measures calculated for short ECG series in distinguishing healthy subjects from patients with congestive heart failure (CHF). Four entropy measures were tested: Approximate Entropy (ApEn), Sample Entropy (SampEn), Fuzzy Entropy (Fuzzy En) and Permutation Entropy (PE), each computed for ECG series of 1000, 500, 250 and 100 RR intervals. We found that with a reduction of the data set length...
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Usability and relational factors in user-perceived quality of online services
PublicationThe purpose of this paper is to identify the key determinants of con-sumer-perceived usability of online service websites and their impact on e-loyalty potential, expressed by customer’s willingness to return and to recommend the website to others. In this experimental usability study fifteen video-recorded users performed specific tasks in financial, travel and health online service websites. After completing the tasks users filled...
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MEASURE OF THE LEVEL OF SOCIO-ECONOMIC DEVELOPMENT IN PROVINCES
PublicationThe scope of Polish macro-economic data for assessing the level of socio-economic development of the country is largely limited because of the regional variation. Therefore there is a need for cyclic selection and the verification of criteria which allow identifying regions with a similar level of socio-economic development or those that clearly differ from the mean values. The aim of the study is to compare Poland’s provinces...
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Predyspozycje zawodowe artysty a cechy przedsiębiorcze
PublicationArtykuł stanowi analizę porównawczą cech osobowości przypisywanych artystom z charakterystyką skutecznego przedsiębiorcy. Podstawą zestawienia jest profil osobowości twórczej, typy osobowości zawodowych oraz profil osobowości przedsiębiorcy. Celem artykułu jest wskazanie zakresu kompetencji, które mogą być niedostępne dla twórców, a które warunkują realizację przedsięwzięć biznesowych. Potwierdzeniem konieczności integracji sylwetki...
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BUILDING INFORMATION MODELING - ANALIZA ZAKRESU I STANU IMPLEMENTACJI W POLSKIEJ BRANŻY BUDOWLANEJ
PublicationProblematyka BIM (Building Information Modeling) jest obecnie tematem wielu publikacji naukowych, ale także przedmiotem działań podejmowanych przez uczestników szeroko pojętego procesu budowlanego. Liczne zalety i szanse płynące ze stosowania technologii BIM w kolejnych etapach cyklu życia obiektu budowlanego, należy rozpatrywać z punktu widzenia inwestora/zamawiającego, architekta, projektanta konstrukcji i instalacji, dewelopera,...
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Impact of Usability Website Attributes on Users’ Trust, Satisfaction and Loyalty
PublicationThis paper presents the results of an experimental study aimed at identifying possible relationships among website usability characteristics, consumer satisfaction, trust and loyalty. These factors regard not only customer satisfaction in a transactional sense, but in the long term they may affect e-customer behavior, opinions, recommendations and attitudes toward using on-line services in general. The study was performed with...
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Influence of Spatial Structures of 316L Stainless Steel on Its Cavitation Erosion Resistance
PublicationCavitation erosion performance of modified macroscopic internal structure 316L stainless steel was investigated. The samples processed by means of SLM method were subjected to cavitation erosion test. The scanning electron microscope Philips 30/ESEM was used to examine morphology of eroded surface.
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Serwisy WWW jako instrument kształtowania relacji z klientem usług medycznych
PublicationWprowadzenie: W artykule podjęto problem oceny użyteczności serwisów WWW i badania postaw lojalnościowych e-klientów korzystających z serwisów WWW usług medycznych. Celem badania była wstępna walidacja metody do oceny jakości użytkowej serwisów WWW usług medycznych oraz określenie związków pomiędzy cechami jakości użytkowej serwisów WWW a cechami relacyjnymi, takimi jak: satysfakcja, chęć powrotu oraz zamiar rekomendowania serwisu...
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Ocena zasobów oraz weryfikacja granic obszaru ochronnego GZWP nr 133 Młotkowo za pomocą numerycznego modelu przepływu wód podziemnych
PublicationModel Głównego Zbiornika Wód Podziemnych powstał w celu dokonania oceny dostępnych zasobów wód oraz zweryfikowania granic strefy ochronnej. GZWP Młotkowo jest położony w południowej części Pojezierza Krajeńskiego. Ze względu na konieczność weryfikacji granic zbiornika wyznaczonych przez Kleczkowskiego i in. (1990) do badań modelowych przyjęto obszar cztery razy większy niż rozmiar GZWP. W celu osiągnięcia najbardziej wiarygodnych...
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Taq DNA polymerase fused with DNA binding protein with increased resistance to inhibitors from clinical samples
PublicationNowadays PCR method is commonly used in molecular diagnostic. However, in many cases PCR is limited, by the presence of inhibitory substances in biological, soil or food samples Efficiency and fidelity of amplification is strongly connected with DNA polymerase and reaction conditions. To meet the requirements of modern diagnostic methods it is essential to seeking for new DNA polymerases with better properties useful in these field....
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Percepcja wiarygodności internetowych serwisów handlowych w kontekście zachowań konsumenckich
PublicationCelem pracy było sprawdzenie, w jaki sposób zachowania konsumenckie determinowane czynnikami społecznymi różnicują percepcję atrybutów kształtujących wiarygodność internetowych serwisów handlowych. Doświadczenie w transakcjach on-line oraz poczucie własnej skuteczności konsumentów nie przyczyniały się do powstawanie różnic. Kobiety, inaczej niż mężczyźni, potrzebowały więcej rekomendacji i były bardziej wyczulone na nieprofesjonalizm...
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Comparision of mechanical properties of nitrided cases and remelted layers of austenitic stainless steel
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Fizyka skoków narciarskich
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