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Search results for: NITRIFYING BACTERIA
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The treatment of wastewater containing pharmaceuticals in microcosm constructed wetlands: the occurrence of integrons (int1–2) and associated resistance genes (sul1–3, qacEΔ1)
PublicationThe aim of this study was to analyze the occurrence of sulfonamide resistance genes (sul1–3) and other genetic elements as antiseptic resistance gene (qacEΔ1) and class 1 and class 2 integrons (int1–2) in the upper layer of substrate and in the effluent of microcosm constructed wetlands (CWs) treating artificial wastewater containing diclofenac and sulfamethoxazole (SMX), which is a sulfonamide antibiotic. The bacteria in the substrate...
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Highly selective impedimetric determination of Haemophilus influenzae protein D using maze-like boron-doped carbon nanowall electrodes
PublicationThis study reports a novel impedimetric immunosensor for protein D detection in purified and bacterial (Haemophilus influenzae, Hi) samples. The detection was based on antigen recognition by anti-protein D antibodies (apD) immobilised at the maze-like boron-doped carbon nanowall electrodes (B:CNW). The B:CNW electrodes were synthesised, and their surface was characterised by scanning electron microscopy (SEM) and X-ray photoelectron...
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Selection of AHI + SC Hybrid Storage Based on Mathematical Models and Load Variation Characteristics
PublicationThe article deals with the design of a hybrid storage consisting of Aqueous Hybrid Ion battery (AHI) and supercapacitor (SC) modules. The selection of components is based on the knowledge of the load profile of the storage and the AHI battery and supercapacitor models. The paper presents the method of selecting components of the storage made of AHI batteries and supercapacitor modules The article includes an example of the hybrid...
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Suppressors of lapC mutation identify new regulators of LpxC, which mediates the first committed step in lipopolysaccharide biosynthesis
PublicationGram-negative bacteria, such as Escherichia coli, are characterized by an asymmetric outer membrane (OM) with lipopolysaccharide (LPS) located in the outer leaflet and phospholipids facing the inner leaflet. E. coli recruits LPS assembly proteins LapB, LapC and LapD in concert with FtsH protease to ensure a balanced biosynthesis of LPS and phospholipids. We recently reported that bacteria either lacking the periplasmic domain of...
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High prevalence of Escherichia coli belonging to the B2+D phylogenetic group in inflammatory bowel disease
PublicationBACKGROUND: It is not clear which species of bacteria may be involved in inflammatory bowel disease (IBD). One way of determining which bacteria might be likely candidates is to use culture-independent methods to identify microorganisms that are present in diseased tissues but not in controls. AIMS: (1) To assess the diversity of microbial communities of biopsy tissue using culture-independent methods; (2) to culture the bacteria...
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REVIEW ON COMPETITION АОB AND NОВ AS AN IMPORTANT ELEMENT FOR COST EFFECTIVE METHODS OF REMOVING NITROGEN FROM WASTEWATER
PublicationThe aim of this paper is analysis of process principles and possible factors used to induce inhibition of nitrite oxidation by NOB (Nitrite Oxidizing Bacteria) which could be obtained during not very well understood processes: so called “shortcut” or “partial” nitrification as well deammonification, by qualitative/quantitative studies of AOB (Ammonium Oxidizing Bacteria) and NOB populations in activated...
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Insights into oral microbiome and colorectal cancer – on the way of searching new perspectives
PublicationMicrobiome is a keystone polymicrobial community that coexist with human body in a beneficial relationship. These microorganisms enable the human body to maintain homeostasis and take part in mechanisms of defense against infection and in the absorption of nutrients. Even though microbiome is involved in physiologic processes that are beneficial to host health, it may also cause serious detrimental issues. Additionally, it has been...
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Ribes nigrum L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles
PublicationSilver nanoparticles (Ag NPs) represent one of the most widely employed metal-based engineered nanomaterials with a broad range of applications in different areas of science. Plant extracts (PEs) serve as green reducing and coating agents and can be exploited for the generation of Ag NPs. In this study, the phytochemical composition of ethanolic extract of black currant (Ribes nigrum) leaves was determined. The main components...
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Zastosowanie testu biologicznego Microtox do oceny skuteczności działania oczyszczalni ścieków bytowo-przemysłowych
PublicationOcena efektywności procesu oczyszczania ścieków z zastosowaniem biotestu Microtox® na przykładzie wybranej oczyszczalni Powszechnie praktykowanym sposobem wprowadzania ścieków oczyszczonych do środowiska jest ich punktowy zrzut do wód powierzchniowych. Różnorodność działalności prowadzonej w ramach współczesnej gospodarki sprawia, że do oczyszczalni docierają nie tylko substancje, których oznaczanie jest wymagane przez przepisy...
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Pharmacomicrobiomics of cell-cycle specific anti-cancer drugs – is it a new perspective for personalized treatment of cancer patients?
PublicationIntestinal bacteria are equipped with an enzyme apparatus that is involved in the active biotrans-formation of xenobiotics, including drugs. Pharmacomicrobiomics, a new area of pharmacology, analyses interactions between bacteria and xenobiotics. However, there is another side to the coin. Pharmacotherapeutic agents can significantly modify the microbiota, which consequently affects their efficacy. In this review, we comprehensively...
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Cloning, expression, purification and characterization of recombinant trehalose synthase from Deinococcus radiodurans.
PublicationTrehalose (α-D-glucopyranosyl-1,1-α-D-glucopyranoside) is a nonreducing disacharide in which the two glucose molecules are linked trough a α-1,1-glycosidic bond. Trehalose is readily hydrolyzed to glucose and can be used as a reserve of that sugar in the cell. The presence of trehalose was found in the cells of fungi and yeasts, bacteria, nematodes, insects, eggs, pupae and some plants. The characteristics of trehalose make it...
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Antibacterial Activity and Cytocompatibility of Bone Cement Enriched with Antibiotic, Nanosilver, and Nanocopper for Bone Regeneration
PublicationBacterial infections due to bone replacement surgeries require modifications of bone cement with antibacterial components. This study aimed to investigate whether the incorporation of gentamicin or nanometals into bone cement may reduce and to what extent bacterial growth without the loss of overall cytocompatibility and adverse effects in vitro. The bone cement Cemex was used as the base material, modified either with gentamicin...
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Characterization of exceptionally thermostable single-stranded DNA-binding proteins from Thermotoga maritima and Thermotoga neapolitana
PublicationBACKGROUND: In recent years, there has been an increasing interest in SSBs because they find numerous applications in diverse molecular biology and analytical methods.RESULTS: We report the characterization of single-stranded DNA binding proteins (SSBs) from the thermophilic bacteria Thermotoga maritima (TmaSSB) and Thermotoga neapolitana (TneSSB). They are the smallest known bacterial SSB proteins, consisting of 141 and 142 amino...
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Isolation and properties of recombinant trehalose synthase
PublicationBiosynthesis of trehalose is carried out via various matabolic pathways, including the use of trehalose synthase. Bacteria of the genus Deionococcus are microorganisms which produce trehalose synthase catalizing conversion of maltose into trehalose. In this work recombinant trehalose synthase from D.radiodurans was characterized.
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A shear stress micromodel of urinary tract infection by the Escherichia coli producing Dr adhesin
PublicationIn this study, we established a dynamic micromodel of urinary tract infection to analyze the impact of UT-segment-specific urinary outflow on the persistence of E. coli colonization. We found that the adherence of Dr+ E. coli to bladder T24 transitional cells and type IV collagen is maximal at lowest shear stress and is reduced by any increase in flow velocity. The analyzed adherence was effective in the whole spectrum of physiological...
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Bee Products and Essential Oils as Alternative Agents for Treatment of Infections Caused by S. aureus
PublicationBacteria of the genus Staphylococcus are important human and veterinary pathogens. A crucial characteristic for this group of bacteria is that they can easily acquire mechanisms of antibiotic resistance for a plethora of antibiotics currently in use for human and animal therapies. Therefore, there is a great need to find novel, non-antibiotic chemotherapeutics with marked antistaphylococcal activity. Promising but still underestimated...
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The effect of UV-C irradiation on lipids and selected biologically active compounds in human milk
PublicationThe effect of UV-C irradiation of human milk on lipid oxidation, content of antioxidants (vitamin C and catalase, CAT) and bactericidal compounds (lysozyme), as well as the total antioxidant capacity (TAC), of the breast milk was investigated. In parallel, the extent of inactivation of some bacteria was also determined. UV-C at doses from 85 to 740 J L−1 caused total inactivation of Escherichia coli and Staphylococcus aureus, but...
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Comparison of disk and tube methods for estimation of biostatic properties of polyurethanes based on synthetic poly[(R,S)-3-hydroxybutyrate
PublicationThe influence of novel aliphatic polyurethanes on growth of Staphyloccocus aureus (SA)in the around of polymer samples has been estimated by disk method. It was staed that the growth of SA was inhibited.The influence of polyurethanes on planctonic Staphyloccocus aureus was also pefrormed by tube method. In this test the slight decrease of bacteria concentration was noticed.
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Dimeric structure of the N-terminal domain of PriB protein from Thermoanaerobacter tengcongensis solved ab initio
PublicationPriB is one of the components of the bacterial primosome, which catalyzes the reactivation of stalled replication forks at sites of DNA damage. The N-terminal domain of the PriB protein from the thermophilic bacterium Thermoanaerobacter tengcongensis (TtePriB) was expressed and its crystal structure was solved at the atomic resolution of 1.09 Å by direct methods. The protein chain, which encompasses the first 104 residues of the...
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Chracteristics of anaerobic ammonium oxidation initated by anaerobic granular sludge
PublicationTwo different mixed sludge, anaerobic granular sludge(R1) and anaerobic granular sludge with mature anaerobic ammonium oxidation (ANAMMOX) sludge (R2 )were used as the inocula in two up-flow reactors to enrich anaerobic ammonia-oxidizing bacteria (AnAOB),respectively.The total nitrogen removal rate of R1 reached 74% in 99 days, while R2 remained above 70% in only 48 days.The content of extracellular polymeric substance (EPS) in...
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Direct determination of cadaverine in the volatile fraction of aerobically stored chicken breast samples
PublicationTo supplement the currently used methods for poultry meat shelf life assessment, it might be necessary to develop a technique for rapid headspace analysis of volatiles with no prior sample preparation step. Biogenic amines, in particular cadaverine, are considered meat spoilage indicators. Described in this article are the results of a preliminary investigation of the applicability of proton transfer reaction mass spectrometry...
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Organizmy obecne w osadzie dennym basenów kąpielowych
PublicationMonitorowanie parametrów wody basenowej jest istotne z uwagi na zdrowie osób korzystających z basenu jak i pracowników obsługi. Istnieją trzy źródła materii organicznej: woda dostarczona do basenu, pasywne zanieczyszczenia wnoszone przez osoby kąpiące się oraz zanieczyszczenia organiczne w postaci moczu i kału. Jakość wody w basenach powinna być porównywalna do jakości wody pitnej. Jakość mikrobiologiczna opiera się na tradycyjnych...
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Charakterystyka genetyczna szczepów Klebsiella pneumoniae izolowanych od pacjentów po przeszczepieniu nerki oraz badanie wpływu leków immunosupresyjnych na ekspresję genów bakteryjnych
PublicationBakterie z gatunku Klebsiella pneumoniae należą do oportunistycznych patogenów odpowiedzialnych za zakażenia szpitalne. Drobnoustroje te wykazują skłonność do oporności wielolekowej i wysokiej wirulencji, co stanowi poważne zagrożenie dla pacjentów nieimmunokompetentnych. Wśród pacjentów po transplantacji nerki zakażenia układu moczowego (ZUM) stanowią najczęstszą przyczynę powikłań infekcyjnych. Mechanizmy molekularne leżące u...
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Szybkie ładowanie samochodu elektrycznego z elektrowni słonecznej
PublicationW artykule przedstawiono stanowisko badawcze umożliwiające realizację procesu szybkiego ładowania samochodu elektrycznego. Wymagana energia jest wyprodukowana przez elektrownię słoneczną i przechowana w stacjonarnej baterii. Rozpatrzono trzy podstawowe sposoby ładowania samochodu elektrycznego: 1) ze stacjonarnej baterii ładowanej z elektrowni słonecznej, 2) ładowarką zasilaną ze stacjonarnej baterii i 3) ładowarką zasilaną ze...
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Effect of operating conditions on N 2 O production in an anammox sequencing batch reactor containing granular sludge
PublicationNitrous oxide (N2O) is one of the gases with the greatest impact in the atmosphere due to its persistence and significant contribution to the greenhouse effect. This study provides an insight into the dynamics of N2O production in wastewater nitrogen removal systems. A 10 L sequencing batch reactor containing enriched anammox biomass was subjected to different operational conditions, i.e., temperature, feed time, NO2 /NH4 þ ratio...
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Two highly thermostable paralogous single-stranded DNA-binding proteins from Thermoanaerobacter tengcongensis
PublicationThe thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. coli overexpression systems. The TteSSB2 and TteSSB3 consist of 153 and 150 amino acids with a calculated molecular...
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Biological and antibacterial properties of chitosan-based coatings with AgNPs and CuNPs obtained on oxidized Ti13Zr13Nb titanium alloy
PublicationDespite numerous studies, the antibacterial efficiency and cytotoxicity of chitosan-based coatings with nanometals need further studies. The purpose of this research was to determine the biological properties of chitosan coatings implemented with either silver nanoparticles (AgNPs) or copper nanoparticles (CuNPs), that were electrophoretically deposited on electrochemically oxidized Ti13Zr13Nb alloy. The SEM tests were used to...
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The Chemical and Biological Properties of Nanohydroxyapatite Coatings with Antibacterial Nanometals, Obtained in the Electrophoretic Process on the Ti13Zr13Nb Alloy
PublicationThe risk of an early inflammation after implantation surgery of titanium implants has caused the development of different antimicrobial measures. The present research is aimed at characterizing the effects of nanosilver and nanocopper dispersed in the nanohydroxyapatite coatings, deposited on the Ti13Zr13Nb alloy, and on the chemical and biological properties of the coatings.The one-stage deposition process was performed by the...
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Comparison of the Efficiency of Deammonification under Different DO Concentrations in a Laboratory-Scale Sequencing Batch Reactor
PublicationThe efficiency of deammonification depends on the cooperation of ammonium oxidizing bacteria and archaea (AOB/AOA), anaerobic ammonium oxidizing bacteria (AnAOB) and the effective suppression of nitrite oxidizing bacteria (NOB) that compete with AnAOB for nitrite (NO2 -N). One of the effective NOB suppression strategies is intermittent aeration. However, it is important to have a good understanding of the optimum dissolved oxygen...
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Biosurfactants - Biodegradability, toxicity, efficiency in comparison with synthetic surfactants
PublicationBiosurfactants are natural surface active agents produced by variety of bacteria yeast and fungi. A review of their biodegradability, toxicity and efficiency is presented in order to compare their properties with synthetic surfactants. Due to their high efficiency in many applications and environmentally friendly character we tried to apply them in oil refining process. The efficiency of biosurfactants in this application was very...
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The influence of biological environment on the appearance of silver-coated implants
PublicationDespite antibiotics preventive treatment before and after implantation, risk of infection is real. The infections at the implant surface developein a few months after applying them into the body. To prevent the development of bacteria and to reduce the risk of infection, implants coated with silver layer come into use. The paper presents the research results in biological environment on vein and orthopaedicimplants covered with...
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Określenie zawartości lotnych kwasów tłuszczowych (LKT) w odciekach pochodzących z mikrobiologicznej degradacji monosacharydów
PublicationOkreślenie zawartości lotnych kwasów tłuszczowych (LKT) w odciekach pochodzących z mikrobiologicznej degradacji monosacharydów. Milena Jabłońska, Maria Rybarczyk, Marta Wisielewska, Marek Lieder Politechnika Gdańska, Wydział Chemiczny, Katedra Technologii Chemicznej Zawartość lotnych kwasów tłuszczowych (LKT) w metabolitach pochodzących z mikrobiologicznego ogniwa paliwowego (MFC – Microbial Fuel Cell) świadczy o rozkładzie...
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Example of utilization of permeabilized microbial cells
PublicationThe benefits of using whole bacterial cells not only exclude expensive, laborious protein isolation and purification but also stabilize enzymes by cytosol components. Increase in activity of the cells can be achieved by cells permeabilization.
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New effective sources of lysostaphin Staphylococcus simulans
PublicationLizostafyna jest białkiem posiadającym zdolność lizowania ścian komórkowych bakterii z rodzaju Staphylococcus. W pracy przedstawiono konstrukcje dwóch kolejnych układów ekspresyjnych umożliwiających otrzymywanie rekombinantowej lizostafyny w komórkach bakterii Escherichia coli, metodykę oczyszczania białka oraz jego charakterystykę biochemiczną.
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The sanitary quality of water and sandy sediments of selected streams and the coastal area of Gdańsk Bay
PublicationPraca prezentuje wyniki badań bakteriologicznych przybrzeżnych wód morskich i plażowych osadów piaszczystych prowadzonych w rejonie ujścia do Zatoki Gdańskiej rzeki Kaczej i Sweliny oraz Potoków Grodowego i Kamiennego. W badaniach określano liczbę bakterii względnie psychrofilnych, mezofilnych oraz bakterii wskaźnikowych (bakterii z grupy coli, coli typu kałowego oraz paciorkowców kałowych). Stwierdzono, iż potoki wnoszą znaczny...
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Molecular identification and genotyping of Staphylococci: genus, species, strains, colnes, lineages, and interspecies exchanges
PublicationStaphylococci are increasingly recognized as etiological agents of many opportunistic human and animal infections, emphasizing the need for a rapid and accurate identification, even to a genotypical level of these bacteria. In the recent years, there has been a significant progress in typing and phylogenetic study of Staphylococcus species. Here, we describe molecular methods used in taxonomy as well as staphylococci characterization....
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Insights into the microbial community of treated wastewater, its year-round variability and impact on the receiver, using cultivation, microscopy and amplicon-based methods
PublicationApart from chemical constituents, wastewater treatment plant (WWTP) effluents also release microorganisms that can be important to the receiving water bodies either from a sanitary point of view, or taking to the account the biogeochemical potential of the recipients. However, little is known about the treated wastewater microbial community, its composition, seasonal changes, functions and fate in the waters of the receiver. Thus,...
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Microorganisms in the air over wastewater treatment plant.
PublicationCelem pracy było aktywności źródeł emisji mikroorganizmów do powietrza na terenie oczyszczalni ścieków. Stwierdzono iż najwyższe liczebności bakterii występowały w rejonie piaskowników i komór recyrkulacji osadu czynnego. Natomiast liczba bakterii w rejonie komór napowietrzania była zbliżona do tła.
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Silver-modified titania with enhanced photocatalytic and antimicrobial properties under UV and visible light irradiation
PublicationCommercial titania photocatalysts were modified with 2 wt% of silver by photodeposition. The properties of the samples were characterized by DRS, XPS, XRD, FE-SEM and STEM. The modified samples exhibited activity under visible light and enhanced activity under UV irradiation for 2-propanol and acetic acid oxidation, respectively. The time-resolved microwave conductivity (TRMC) analysis indicated that enhanced activity (2.5–8-fold...
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Escherichia coli Strains with Virulent Factors Typical for Uropathogens were Isolated from Sinuses from Patients with Chronic Rhinosinusitis—Case Report
PublicationEscherichia coli were isolated from three patients with chronic rhinosinusitis (CRS) by intraoperative sinus tissue biopsy. Taking into account the unusual replicative niche and previous treatment failures, it was decided to focus on the virulence and drug resistance of these bacteria. The strains turned out to be multi-sensitive, but the rich virulence factors profile of bacteria typical for phylogenetic group B2 deserved attention....
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Modeling N2O behavior during denitrification with different carbon sources and electron acceptors
PublicationA mathematical model was developed for identification of the most important mechanism leading to nitrous oxide (N2O) accumulation in denitrifying activated sludge systems. The activated sludge model No. 2d (ASM2d) was extended to include intermediate three steps of the denitrification process. The formation and accumulation of nitric oxide (NO) was assumed to be very low in the performed laboratory experiments. These experiments...
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Homocitrate synthase gene as a new molecular target in diagnostics of Candida spp. infections
PublicationNew potential molecular target which will be investigated during this research (homocitrate synthase gen) is the innovative target other than ribosomal DNA. This gene encodes enzyme present only in fungi and some Archaea what significantly increases specificity. It also reduces risk of contamination or influence of other factors (reaction with other DNA present in sample derived from bacteria or host cells) what excludes false...
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The use of various species of fungi in biofiltration of air contaminated with odorous volatile organic compounds
PublicationAir pollution with odorous compounds is a significant social and environmental problem. This paper presents biological deodorization methods. The attention is focused on the application of biotrickling filters for air deodorization. Principles of their operation are discussed, indicating the key role of the selection of microorganisms responsible for the degradation of odorous compounds. A literature overview of the used fungal...
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Antimicrobial resistance of fecal indicators in disinfected wastewater
PublicationThe main objective of the study was to assess the potential of three systems (UV irradiation, ozonation, and micro/ultrafiltration) operated in a pilot scale in removal of antimicrobial-resistant fecal bacteria from secondary effluent of the local wastewater treatment plant (700,000 population equivalent). The effectiveness of the processes was analysed using the removal ratio of fecal indicators (Escherichia coli and Enterococcus...
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A CNN based coronavirus disease prediction system for chest X-rays
PublicationCoronavirus disease (COVID-19) proliferated globally in early 2020, causing existential dread in the whole world. Radiography is crucial in the clinical staging and diagnosis of COVID-19 and offers high potential to improve healthcare plans for tackling the pandemic. However high variations in infection characteristics and low contrast between normal and infected regions pose great challenges in preparing radiological reports....
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Mutual Interaction between Temperature and DO Set Point on AOB and NOB Activity during Shortcut Nitrification in a Sequencing Batch Reactor in Terms of Energy Consumption Optimization
PublicationRecently, many wastewater treatment plants (WWTPs) have had to deal with serious problems related to the restrictive requirements regarding the euent quality, as well as significant energy consumption associated with it. In this situation, mainstream deammonification and/or shortened nitrification-denitrification via nitrite (so-called “nitrite shunt”) is a new promising strategy. This study shows the mechanisms and operating conditions...
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pH-dependent composite coatings for controlled drug delivery system - Review
PublicationNowadays in case of long-term implants, the most common postoperative complications are bacterial infections, which in consequence may provoke loos- ening of the implants in the primary phase of stabilization. Bacterial infections are currently the most frequent cause of revision surgery of the implants such as hip joint endoprosthesis, knee joint endoprosthesis and dental implants. In order to provide the local and long-term antibacterial...
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Effect of aeration of antibiotic-loaded bone cement on its properties and bactericidal effectiveness
PublicationBACKGROUND: Antibiotic-loaded bone cements are now widely used in medicine. They are able to locally deliver antibiotic particles and they allow treat or protect against infection. It is assumed that the bactericidal effectiveness of bioactive bone cements depend on the parameters of its production. Hence, the aim of this study was to check the effect of aeration of bone cement before mixing the components on its properties as...
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Factors causing degradation of sulfadimetoxine
PublicationSulfadimetoxine is a representative of sulfonamide drugs which presence inhibits growth of activated sludge bacteria, rhat is necessary for the effective mineralization of organic and inorganic contaminants. Therefore factors which con prevent this situation are searched. Aim of this study is selection of conditions causing efficien degradation of the drug- sulfadimetoxine. The impact of such aspects as : the type of oxidizing...
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Importance of the combined effects of dissolved oxygen and pH on optimization of nitrogen removal in anammox-enriched granular sludge
PublicationThe combined effects of dissolved oxygen (DO) and pH on nitrogen removal were investigated in a laboratory-scale sequencing batch reactor (SBR) with anammox-enriched granular sludge obtained from a nitritation/anammox system. The highest specific nitrogen removal rate (SNRR) (1.1 gN gVSS−1 d−1) was observed under non-aerated conditions, resulting in the nitrogen removal efficiency of 81.6%. Although nitrogen removal was readily...