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Novel staphylococci nucH taxonomical marker used in identification of human-associated Staphylococcus succinus subsp. casei
PublikacjaThe aim of our study was to assess the sequencing of unique nucH gene fragment based on performed bioinformatics analysis as a novel diagnostic method for the identification of difficult to identify staphylococcal human pathogenic strains. Initially, PCR-RFLP-rrn analysis specific to the spacers between 16SrDNA and 23SrDNA followed by HhaI restriction analysis was performed. Further, sequencing of nucH and 16S rDNA genes fragments...
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Analysis the Parameters of the Adaptive Controller for Quality Control of Dissolved Oxygen Concentration
PublikacjaThe wastewater treatment plant can be considered a dynamic large scale complex system, in which the most important control parameter is the dissolved oxygen concentration in the aerobic zone. The air is supplied to this zone by the aeration system. In the paper, both the sequencing batch reactor and the aeration system are modelled and used as plant of control performed by the cascade nonlinear adaptive control system extended...
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Meiothermus ruber cells as β-galactosidase activity biocatalyst
PublikacjaThe current study allowed to establish the possibility of using whole cells of thermophilic bacteria Meiothermus ruber as a biocatalyst with β-galactosidase activity. β-galactosidases are used for hydrolysis of lactose as well as for oligosaccharides synthesis. The advantages of using whole bacterial cells catalysis is not only elimination of tedious, expensive protein isolation and/or purification but also stabilization of enzymes...
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From fluid mechanics backgrounds to modern field theory
PublikacjaOur presentation keeps a historical line of reasoning, since we start from old concepts of fluid mechanics and finish on concepts of modern field theory. We want to show that some facts from the nature phenomena, which have firstly been discovered on the ground of fluid mechanics, were next incorporated into physics and later become the important pattern for whole mathematical physics. Especially, well-known continuum models, which...
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Increasing K-Means Clustering Algorithm Effectivity for Using in Source Code Plagiarism Detection
PublikacjaThe problem of plagiarism is becoming increasingly more significant with the growth of Internet technologies and the availability of information resources. Many tools have been successfully developed to detect plagiarisms in textual documents, but the situation is more complicated in the field of plagiarism of source codes, where the problem is equally serious. At present, there are no complex tools available to detect plagiarism...
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ABSORPCJA, METABOLIZM I ROLA BIOLOGICZNA KWASÓW NUKLEINOWYCH OBECNYCH W ŻYWNOŚCI
PublikacjaKwasy nukleinowe należą do niedocenianych składników żywności, szczególnie surowej lub nisko przetworzonej. W niniejszej publikacji skupiono się na omówieniu przemian, jakim podlegają kwasy nukleinowe w przewodzie pokarmowym człowieka, procesie absorpcji nukleotydów oraz nukleozydów z przewodu pokarmowego, a także przedstawiono podstawowe etapy ich metabolizmu w komórkach organizmu. Produkty trawienia kwasów nukleinowych stanowią...
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Przydział narzędzi obróbkowych a efektywność szeregowania zadań produkcyjnych
PublikacjaThe paper addresses issues concerning the analysis of tool flow within a multi-machine machining cell, designated to small batch manufacturing a definite spectrum of prismatic parts. The approach utilises a method for job and tool allocation to work centres with limited number of machines and capacity of tool resources, based on the analysis of formalised relations: job - tool sets required. Selected allocation strategies are considered...
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A mechanistic model for fate and removal of estrogens in biological nutrient removal activated sludge systems
PublikacjaTwo estrogen fate and transformation models were integrated with a comprehensive activated sludge model (ASM) to predict estrogen removal based on biomass and solids production. Model predictions were evaluated against published full-scale plant data as well as results from a laboratory-scale sequencing batch reactor (SBR) fed synthetic wastewater. The estrogen fate model relating the rate of total estrogen degradation to soluble...
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Example of utilization of permeabilized microbial cells
PublikacjaThe benefits of using whole bacterial cells not only exclude expensive, laborious protein isolation and purification but also stabilize enzymes by cytosol components. Increase in activity of the cells can be achieved by cells permeabilization.
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Investigation of Microbes in the Stratosphere – Stardust Project
PublikacjaThe stratospheric microbiota has been investigated many times using the methods of classical microbiology. In this research a few modern methods, including NGS sequencing and multiple displacement amplification of DNA, were in use. The analysis of metagenome helped to determine the content of various species of bacteria in the sample collected in the stratosphere by a hydrogen-filled balloon holding...
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Euro 2012 in Gdańsk, Poland. Is it worth using public funds?
PublikacjaThe aim of this study is to estimate the willingness - to - pay (WTP) among the community of the Pomeranian region, Poland in connection with intangible benefits and costs st emming from hosting the Euro 2012 in Gdansk. A survey conducted among 407 respondents was the source of information. The results of the study show that the average value of WTP benefit for the whole sample was 45,72 PLN2012 and WTP cost...
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Comparative Study of Machining Technology Selection to Manufacture Large-Size Components of Offshore Constructions
PublikacjaThe focus of this paper is on process planning for large parts manufacture in systems of definite process capabilities, involving the use of multi-axis machining centres. The analysis of machining heavy mechanical components used in off-shore constructions has been carried out. Setup concepts applied and operation sequences determined in related process plans underwent studies. The paper presents in particular a reasoning approach...
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Inhibition of the granular anammox process by the high concentration of free ammonia.
PublikacjaHigh concentrations of ammonia and nitrite, typical for reject water treatment in the anammox process with the use of high temperatures and pH, may cause free ammonia (FA) inhibition. In this study, the inhibitory effect of FA on anammox process rate was investigated in a bench-scale sequencing batch reactor (SBR) with granular anammox biomass. Results of the study present that the anammox process could be successfully operated...
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Designing control strategies of aeration system in biological WWTP
PublikacjaThe paper presents the complete design processes of a novel aeration control systems in the SBR (Sequencing Batch Reactor) wastewater treatment plant (WWTP). Due to large energy expense and high influence on biological processes, the aeration system plays a key role in WWTP operation. The paper considers the aeration system for a biological WWTP located in the northeast of Poland. This system consists of blowers, the main collector...
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Simple and low-cost wireless voting system
PublikacjaThis paper presents the concept of a simple and low-cost wireless voting system working on the 868 MHz frequency band. Described system is dedicated to general shareholders assemblies but it can be easily adapted for other applications. The main advantage is its simplicity and mobility as it consists solely of three components - voting modules, base station and a PC application from which the whole system is managed. This architecture...
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Simple and low-cost wireless voting system
PublikacjaThis paper presents the concept of a simple and low-cost wireless voting system working in the 868 MHz frequency band. The described system is dedicated to general shareholders assemblies but it can be easily adapted for other applications. The main advantage is its simplicity and mobility as it consists solely of three components - voting modules, a base station and a PC application from which the whole system is mamaged. This...
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Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species
PublikacjaPurpose Fungi of Scopulariopsis and Microascus genera cause a wide range of infections, with S. brevicaulis being the most prevalent aetiological agent of mould onychomycosis. Proper identification of these pathogens requires sporulating culture, which considerably delays the diagnosis. So far, sequencing of rDNA regions of clinical isolates has produced ambiguous results due to the lack of reference sequences in publicly available...
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Structure and the Biochemical Potential of the Bacterial Community in Wastewater Treatment Plant Effluent and in Receiving Waters: Northern Poland Case Study
PublikacjaWastewater treatment processes are monitored mainly in terms of biogenic substance removal efficiency. Little is known about the treated wastewater microbiome and its influence on the recipient. In this study, a wide range of methods (cultivation, microscopic analysis, New Generation Sequencing and Real Time PCR) were employed to determine the bacterial community composition of the treated wastewater and its receiver. Preliminary...
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Simplified stability analysis of steel cylindrical silos with corrugated walls and vertical columns
PublikacjaIn the paper a simplified stability analysis of steel cylindrical silos composed of corru¬gated walls and vertical open-sectional columns was proposed. The whole 3D silo was modeled as a single column resting on elastic foundation provided by the silo walls. In proposed formula for the calculation of the foundation stiffness the curvature of the silo walls was taken into account. Three different analyses were carried out using...
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CORROSION TESTING OF SAMPLES ACCORDING TO ASTM G36
PublikacjaAt the end of the study, samples were found to have structures characteristic for intercrystalline corrosion. This indicates that the samples provided (fragments of the whole) were sensitized before starting the study in boiling MgCl2.On cross sections, fine cracks are observed on all samples tested.
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Monument of History in Gdynia - Problems with Protection of the Main Representative Axis of the City
PublikacjaThe spatial idea of the main representative axis was presented in 1938 in the project of the Representative District of Gdynia and partly realised in 1938-1939. Although the whole area was included in the Gdynia's Monument of History, now its spatial integration is on thread by the controversial current development plans
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MIC1-MAG1-SAG1 chimeric protein, a most effective antigen for detection of human toxoplasmosis
PublikacjaThis study describes a Toxoplasma gondii IgG ELISA test based on a new chimeric antigen containing three immunodominant regions from MIC1, MAG1 and SAG1 proteins of a parasite and shows that this test is useful for diagnostic purposes and may replace the lysed and whole-cell antigens.
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Case Study on the Use of Unmanned Aerial Vehicles for the Maintenance of Ocean Engineering Facilities
PublikacjaThe article presents the concept of using innovative unmanned aerial vehicles (UAVs), including autonomous UAV-I for inspection activities, auxiliary and transport works in the processes of servicing large-scale ocean engineering structures in the offshore part of the sea. The proposed areas of application of UAV-I devices and key technologies determining the possibility of using these vehicles in difficult operating conditions...
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Buckling Analysis of Cold Formed Silo Column
PublikacjaThe paper is devoted to stability analysis of different models of steel cold formed silo column. The steel cylindrical silos are often composed of corrugated walls and vertical open-sectional columns uniformly placed along the silo circumference. Both the whole 3D silo, a simplified model consisting of one column with a part of the silo walls, and a single column resting on elastic foundation provided by the silo walls were analyzed....
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The Application of Fibres from Recycled Postconsumer Tyres for Concrete Reinforcement
PublikacjaPoland as a Member State is obliged to implement the EU specific environmental legislation focused on the reduction, transport and treatment of waste. The Landfill Directive prohibited the landfilling of whole tyres and their by-products. Although the EU Directive of Waste limits the use of tyres as secondary fuel, Poland is still the EU leader in burning tyres in cement kilns. Post-consumer tyres are utilised in a number of...
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Antiproliferative, Antiangiogenic, and Antimetastatic Therapy Response by Mangiferin in a Syngeneic Immunocompetent Colorectal Cancer Mouse Model Involves Changes in Mitochondrial Energy Metabolism
PublikacjaIn spite of the current advances and achievements in cancer treatments, colorectal cancer (CRC) persists as one of the most prevalent and deadly tumor types in both men and women worldwide. Drug resistance, adverse side effects and high rate of angiogenesis, metastasis and tumor relapse remain one of the greatest challenges in long-term management of CRC and urges need for new leads of anticancer drugs. We demonstrate that CRC...
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ESTIMATION OF THE WILLINGNESS-TO-PAY FOR PRESERVING THE FOOTBALL ARENA IN GDANSK
PublikacjaThe European Football Championship (Euro 2012) organized in Poland became the pretext for a number of infrastructural changes. A high level of expenditure makes the Polish event the most expensive among events of this magnitude. The fact that these changes were 100% financed by public funds raises the question of whether these funds were used in a substantiated way. Therefore, the aim of this study is...
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The Factors Affecting Group Identity of Cluster Structures
PublikacjaThe paper provides a new approach to cluster analysis, basing on a sociologically rooted concept of identity. The authors state that identity in cluster structures is formed by two main groups of factors – uncontrollable or slightly controllable factors (identity mix) and factors that can be fully controlled by a cluster initiative (corporate identity mix). It means that the cluster coordinator is able to consciously build the...
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Optoelectronic device for hematocrit measurements
PublikacjaAn optoelectronic system for measurements of hematocrit level (HCT) in the whole human blood is presented. Proposed system integrates a dedicated optoelectronic sensor, a microcontroller and a small LCD display in a low cost, battery-powered, handheld device. Chosen method for determining blood hematocrit level is based on optical properties of whole blood in visible and NIR wavelength range. Measurements with the use of proposed...
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Overhead wires detection by FPGA real-time image processing
PublikacjaThe paper presents design and hardware implementation of real-time image filtering for overhead wires detection divided on image processing and results presentation blocks. The image processing block was separated from the whole implementation, and its delay and hardware complexity was analysed. Also the maximum frequency of image processing of the proposed implementation was estimated.
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Preparation, composition and properties of fish silage produced with post-coagulation sludge
PublikacjaPost-coagulation sludge was added to fish fydrolyzates (silage). Silage was prepared using whole sprats. Technology of the process has been modified and sulphuric acid was replaced with phosphoric acid. The objective was to evaleuate the quality of produced silage during storage, particulary the changes of peroxide value and amount of free acids in silage fat.
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Praseodymium substituted lanthanum orthoniobate: Electrical and structural properties
PublikacjaThe results of ionic transport and structural measurements for the lanthanum orthoniobate doped by praseodymium are presented and discussed. The influence of calcium co-doping on these properties has also been analyzed. The results suggest the predominant protonic conductivity for the investigated system in the whole range of investigated temperatures. The influence of calcium co-doping on phase purity is analyzed.
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Chronic Rhinosinusitis—Microbiological Etiology, Potential Genetic Markers, and Diagnosis
PublikacjaChronic rhinosinusitis (CRS) is a significant public health problem. Bacterial colonization and impaired mucociliary clearance play a significant role in the inflammatory process. Several inflammatory pathways and host defense elements are altered in CRS, which may contribute to observed differences in the microbiome. To date, researching CRS has been difficult due to limited access to the studied tissue and a lack of available...
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Improving SBR Performance Alongside with Cost Reduction through Optimizing Biological Processes and Dissolved Oxygen Concentration Trajectory
PublikacjaAuthors of this paper take under investigation the optimization of biological processes during the wastewater treatment in sequencing batch reactor (SBR) plant. A designed optimizing supervisory controller generates the dissolved oxygen (DO) trajectory for the lower level parts of the hierarchical control system. Proper adjustment of this element has an essential impact on the efficiency of the wastewater treatment process as well...
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A comprehensive approach to SBR modelling for monitoring and control system design
PublikacjaThe aim of this research is to provide a comprehensive description of the Sequencing Batch Reactor (SBR) modelling for monitoring, control, and plant operational optimisation validation. The paper provides a detailed modelling of the SBR, its components and constituent processes. For this purpose, the mass balance principle and continuity equations were provided with a reactive term implemented using Activated Sludge Model (ASM)....
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Impedance Spectra of RC Model as a Result of Testing Pulse Excitation Measurement Method Dataset
PublikacjaThe dataset titled Impedance spectra of RC model as a result of testing pulse excitation measurement method contains the impedance spectrum of an exemplary test RC model obtained using pulse excitation. The dataset allows presentation of the accuracy of the impedance spectroscopy measuring instrument, which uses the pulse excitation method to shorten the time of the whole spectrum acquisition.
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Widespread theta synchrony and high-frequency desynchronization underlies enhanced cognition
PublikacjaThe idea that synchronous neural activity underlies cognition has driven an extensive body of research in human and animal neuroscience. Yet, insufficient data on intracranial electrical connectivity has precluded a direct test of this hypothesis in a whole-brain setting. Through the lens of memory encoding and retrieval processes, we construct whole-brain connectivity maps of fast gamma (30-100 Hz) and slow theta (3-8 Hz) spectral...
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An Approximation of the Zero Error Capacity by a Greedy Algorithm
PublikacjaWe present a greedy algorithm that determines a lower bound on the zero error capacity. The algorithm has many new advantages, e.g., it does not store a whole product graph in a computer memory and it uses the so-called distributions in all dimensions to get a better approximation of the zero error capacity. We also show an additional application of our algorithm.
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An Approximation of the Zero Error Capacity by a Greedy Algorithm.
PublikacjaWe present a greedy algorithm that determines a lower bound on the zero error capacity. The algorithm has many new advantages, e.g., it does not store a whole product graph in a computer memory and it uses the so-called distributions in all dimensions to get a better approximation of the zero error capacity. We also show an additional application of our algorithm.
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Fungal Typin Methods
PublikacjaThe broad application of the molecular techniques in mycoses diagnosis is related to increase of infections caused by fungi in many countries. The oldest typing methods relaying on fenotypic observation, physiological and biochemical examination have had very limited importance from decades. Novadays, the molecular biology methods took their place. Most of the genotyping methods have been devoleped to be applied for typing of bacteria...
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Evaluation of affective intervention process in development of affect-aware educational video games
PublikacjaIn this paper initial experiences are presented on implementing specific methodology of affective intervention design (AFFINT) for development of affect-aware educational video games. In the described experiment, 10 student teams are to develop affect-aware educational video games using AFFINT to formalize the whole process. Although all projects are still in progress, first observations and conclusions may already be presented.
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Application, design and operation of constructed wetland systems: case studies of systems in the Gdańsk region, Poland
PublikacjaDifferences in operation result from physical, chemical and biological conditions, which directly influence transformations in the whole aquatic-matrix-plants environment. Depending on the quantity of inflowing organic matter load and the rate of biochemical processes, the contaminations could be removed or/and retuned in the system. From this point of view the facility should be designed and operated in such way that the highest...
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On zero-error codes produced by greedy algorithms
PublikacjaWe present two greedy algorithms that determine zero-error codes and lower bounds on the zero-error capacity. These algorithms have many advantages, e.g., they do not store a whole product graph in a computer memory and they use the so-called distributions in all dimensions to get better approximations of the zero-error capacity. We also show an additional application of our algorithms.
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Triazoloacridone C-1305 impairs XBP1 splicing by acting as a potential IRE1α endoribonuclease inhibitor
PublikacjaInositol requiring enzyme 1 alpha (IRE1α) is one of three signaling sensors in the unfolding protein response (UPR) that alleviates endoplasmic reticulum (ER) stress in cells and functions to promote cell survival. During conditions of irrevocable stress, proapoptotic gene expression is induced to promote cell death. One of the three signaling stressors, IRE1α is an serine/threonine-protein kinase/endoribonuclease (RNase) that...
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Integration of the sulfate reduction and anammox processes for enhancing sustainable nitrogen removal in granular sludge reactors
PublikacjaThe Anammox and Sulfate Reduction Ammonium Oxidation processes were compared in two granular sequencing batch reactors operated for 160 days under anammox conditions. It was hypothesized that increasing the concentration of SO42− may positively influence the rate of N removal under anaerobic conditions and it was tested whether SO42− reduction and anammox occur independently or are related to each other. The cooperation of N-S...
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Metody metagenomiczne w badaniu mikrobiomu jelitowego człowieka
PublikacjaMikroorganizmy zamieszkujące ciało człowieka to nie tylko komensale i groźne patogeny, ale także organizmy symbiotyczne pełniące kluczową rolę w zachowaniu homeostazy gospodarza. Badania metagenomiczne wykorzystujące techniki sekwen-cjonowania nowej generacji i metody bioinformatyczne pozwalają na dogłębne po-znanie mikrobiomu ludzkiego i poszukiwanie jego związku ze stanem zdrowia czło-wieka. Celem pracy jest omówienie różnych...
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Etyka marketingu - czy marketing jest etyczny?
PublikacjaOd jakiegoś czasu pojawiają się krytyczne głosy wobec marketingu, jako działań nieetycznych, wycelowanych przeciwko klientom i społeczeństwu jako całości. Można stwierdzić, że wizerunek marketingu jest w tym kontekście negatywny. Celem artykułu jest próba odpowiedzi na pytanie czy marketing jest etyczny. Stwierdzono, że istotą marketingu jest dbanie o dobro interesariuszy przedsiębiorstw oraz łączenie interesu firmy, klientów...
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Partial Molar Volumes of Tetrabutylammonium Perchlorate and Tetraphenylborate in N,N-dimethyl-acetamide, Triethylphosphate and Dimethyl Sulfoxide
PublikacjaThe densities of tetrabutylammonium perchlorate and tetrabutylammonium tetraphenylborate solutions in N,N-dimethylacetamide, acetonitrile, triethylphosphate, and dimethyl sulphoxide have been measured over the whole electrolyte concentration range at 20, 25, and 30°C. From these densities apparent molar volumes and partial molar volumes of the salts at infinite dilution have been evaluated and used to determine the respective...
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Participatory projects in urban area – opportunities and limitations on the example of 2013 Podwórkowa Rewolucja
PublikacjaParticipatory projects implemented in urban space have a group of supporters and opponents. The aim of this article is to define the possibilities and limitations of this type of spatial activities on the example of the 2013 Podwórkowej rewolucji in the development of the Edukacja obywatelska w działaniu project in cooperation with the Social Innovation Foundation and its Neighborhood House - the Gościna Przystań, where the inhabitants...
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Explanation of Reduction of Pressure Forces in Turbine Chamber Seals
PublikacjaThe paper describes a chamber seal reducing the aerodynamic forces created in shroud seals. Asymmetrical pressure distribution (due to rotor-stator eccentricity) in the blade tip clearance leads to the creation of pressure forces acting on the blade shroud. By increasing the volume above the shroud, the pressure distribution becomes more uniform on the whole shroud circumference and the effect of rotor eccentricity is reduced....