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Wyniki wyszukiwania dla: dna mismatch enrichment
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ADSRRS-fingerprinting and PCR MP techniques for studies of intraspecies genetic relatedness in Staphylococcus aureus
PublikacjaW pracy pokazano użyteczność dwóch nowych metod do typowania molekularnego Staphylococcus aureus, ADSRRS-fingerprinting (Amplification of DNA fragments Surrounding Rare Restriction Sites) i PCR MP (PCR melting profile). Przebadano 37 szczepów Staphylococcus aureus izolowanych od pacjentów z czyracznością. Jako metodę referencyjną zastosowano REA-PFGE. Okazało się, że izolaty Staphylococcus aureus z nosa od niektórych pacjentów...
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Hydrodynamical loads on a floating dock towed in sea conditions
PublikacjaPrzedstawiono i analizowano charakterystyki amplitudowe (RAO) oraz prognozy krótko i długoterminowe hydrodynamicznych reakcji doku pływającego holowanego w warunkach morskich na akwenach Bałtyku i Morza Północnego. Przedmiotem badań były: nurzania i kołysania wzdłużne, ciśnienia dynamiczne indukowane w różnych częściach dna doku, pionowy moment gnący i opór doku holowanego na falach czołowych. Charakterystyki RAO rozpatrywanych...
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Description of the hydrodynamic pressure field function around the ship hull
PublikacjaW artykule przedstawiono metodę aproksymacji pola hydrodynamicznego okrętu, wykorzystując pole obliczone metodą elementów skończonych. Punkty kontrolne umieszczono w środku paneli rozmieszczonych na kadłubie statku, na powierzchni dna morskiego oraz na powierzchni swobodnej; w p-ktach tych znajdują się osobliwości w postaci źródeł, upustów i/lub dipoli. Przedstawione wyniki obliczeń zostały uzyskane przy użyciu tych pierwszych...
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Influence of temperature and interactions with ligands on dissociation of dsDNA and ligand-dsDNA complexes of various types of binding : an electrochemical study.
PublikacjaSeveral medicinally important compounds that bind to dsDNA strands via intercalation (C-1311, C-1305, EtBr), major groove binding (Hoechst 33258) and covalent binding (cis-Pt) were examined. The obtained results suggest that both the transfer of conformation B to C and the denaturation process, for the ligand-dsDNA complexes, except for covalently bound cis-Pt, took place at higher temperatures compared to the unbound helix. Furthermore,...
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VUV photofragmentation of the six-membered heterocyclic molecules containing oxygen
PublikacjaCancer is a significant public health concern worldwide, which results in millions of deaths each year. The standard cure routine for cancer is surgery, and nowadays, radiotherapy or a hadrontherapy. Depending on the type of cancer, patients may undergo additional treatment, including targeted therapy. A combination of radio- or hadron-therapy with proper drug treatment can inhibit the proliferation of cancer cells and thus can...
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The methylome and transcriptome of fetal skin: implications for scarless healing
PublikacjaAim: Fetal skin is known to heal without scarring. In mice, the phenomenon is observed until the 16–17 day of gestation – the day of transition from scarless to normal healing. The study aims to identify key methylome and transcriptome changes following the transition. Materials & methods: Methylome and transcriptome profiles were analyzed in murine dorsal skin using microarray approach. Results & conclusion: The genes associated...
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The role of low-energy electrons in the charging process of LISA test masses
PublikacjaThe estimate of the total electron yield is fundamental for our understanding of the test-mass charging associated with cosmic rays in the Laser Interferometer Space Antenna (LISA) Pathfinder mission and in the forthcoming gravitational wave observatory LISA. To unveil the role of low energy electrons in this process owing to galactic and solar energetic particle events, in this work we study the interaction of keV and sub-keV...
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Towards Knowledge Formalization and Sharing in a Cognitive Vision Platform for Hazard Control (CVP-HC)
PublikacjaHazards are present in all workplaces and can result in serious injuries, short and long-term illnesses, or death. In this context, management of safety is essential to ensure the occupational health of workers. Aiming to assist the safety manage-ment process, especially in industrial environments, a Cognitive Vision Platform for Hazard Control (CVP-HC) has been proposed. The CVP-HC is a scalable yet adaptable system capable of...
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Urządzenie do usuwania gazów bojowych z dna morskiego
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Polymeric metal‐containing ionic liquid sorbent coating for the determination of amines using headspace solid‐phase microextraction
PublikacjaThis study describes the design, synthesis, and application of polymeric ionic liquid sorbent coatings featuring nickel metal centers for the determination of volatile and semivolatile amines from water samples using headspace solidphase microextraction. The examined polymeric ionic liquid sorbent coatings were composed of two ionic liquid monomers (tetra(3-vinylimidazolium)nickel bis[(trifluoromethyl)sulfonyl]imide [Ni2+(VIM)4]...
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Personalized avatar animation for virtual reality
PublikacjaThe paper presents a method for creating a personalized animation of avatar for virtual reality application such as multiplayer on-line games. Animation is stored in a simplified version, containing only keyframes for important avatar poses. This version defines key movements, i.e. roughly describes the avatar's action. Animation is enriched by the user with new motion phases utilizing fuzzy descriptors.Various degrees of motion...
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Nanoparticle-assisted biohydrogen production from pretreated food industry wastewater sludge: Microbial community shifts in batch and continuous processes
PublikacjaBiohydrogen production from industrial waste has gained a significant attention as a sustainable energy source. In this study, the enrichment of biohydrogen production from pretreated dissolved air flotation (DAF) sludge, generated from food industry wastewater treatment plants, was investigated using SiO2@Cu-Ag dendrites cor- e–shell nanostructure (NS). The effect of NS on the changes of the microbial community and biohydrogen...
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Interactions between bioactive components determine antioxidant, cytotoxic and nutrigenomic activity of cocoa powder extract
PublikacjaNumerous studies have shown, rather disappointingly, that isolated bioactive phytochemicals are not as biologically effective as natural plant products. Such a discrepancy may be explained by the concept of food synergy, which was verified in this research for cocoa extract versus its major components with regard to cancer chemoprevention. The evaluation embraced the relationship between redox properties evaluated in cell-free systems...
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Multicriteria decision analysis in ranking of analytical procedures for aldrin determination in water
PublikacjaThe study presents the possibility of multi-criteria decision analysis (MCDA) application when choosing analytical procedures with low environmental impact. A type of MCDA, Preference Ranking Organization Method for Enrichment Evaluations (PROMETHEE), was chosen as versatile tool that meets all the analytical chemists – decision makers requirements. Twenty five analytical procedures for aldrin determination in water samples (as...
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Assembly of Lipopolysaccharide in Escherichia coli Requires the Essential LapB Heat Shock Protein
PublikacjaHere, we describe two new heat shock proteins involved in the assembly of LPS in Escherichia coli, LapA and LapB (lipopolysaccharide assembly protein A and B). lapB mutants were identified based on an increased envelope stress response. Envelope stress responsive pathways control key steps in LPS biogenesis and respond to defects in the LPS assembly. Accordingly, the LPS content in ΔlapB or Δ(lapA lapB) mutants was elevated, with...
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Heavy Metals in Sediments of Urban Streams: Contamination and Health Risk Assessment of Influencing Factors
PublikacjaSediments of two urban streams in northern Poland outflowing to the Baltic Sea were assessed to explain the spatial variation in relation to urbanization level of the catchment, the role of retention tanks (RTs) and identification of pollution level. During the 3 month period of investigation sediment samples were collected from the inflow (IN) and outflow (OUT) of six RTs located on streams for flood protection. Six heavy metals...
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Optimised five-hour multiplex PCR test for the detection of Tinea ungium: performance in a routine PCR laboratory
PublikacjaWe recently published the development of a 5-hour multiplex PCR test for the detection of dermatophyte nail infection. We have optimized this test by inclusion of an inhibition control and evaluated the test in a routine laboratory when compared to the conventional microscopy and culture. A total of 109 clinical samples received at the mycology reference lab at Statens Serum Institute were included. The samples were divided equally...
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Similarities and differences in d6 low-spin ruthenium, rhodium and iridium half-sandwich complexes: synthesis, structure, cytotoxicity and interaction with biological targets
PublikacjaIn this paper, we discussed the similarities and diferences in d6 low-spin half-sandwich ruthenium, rhodium and iridium complexes containing 2,2′-biimidazole (H2biim). Three new complexes, {[RuCl(H2biim)(η6 -p-cymene)]PF6}2·H2O (1), [(η5 - Cp)RhCl(H2biim)]PF6 (2), and [(η5 -Cp)IrCl(H2biim)]PF6 (3), were fully characterized by CHN, X-ray difraction analysis, UV–Vis, FTIR, and 1 H, 13C and 15N NMR spectroscopies. The complexes exhibit...
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ARGENTUM Pochodne akrydyny i akrydonu o aktywności inhibicyjnej wobec grzybowej topoizomerazy DNA jako potencjalne leki przeciwgrzybowe
ProjektyProjekt realizowany w Katedra Technologii Leków i Biochemii zgodnie z porozumieniem DEC-24/2020/IDUB/I.3.3 z dnia 2020-11-17
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Molekularny mechanizm wpływu stresu oksydacyjnego na stabilność strukturalną i dynamikę kompleksów telomerowego DNA z białkami
ProjektyProjekt realizowany w Katedra Chemii Fizycznej zgodnie z porozumieniem 0059/DIA/2014/43 z dnia 2014-09-18
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Ocena aktywności immunogennej i immunoprotekcyjnej szczepionek DNA kodujących wieloantygenowe białka chimeryczne i fuzyjne Toxoplasma gondii
ProjektyProjekt realizowany w Katedra Biotechnologii Molekularnej i Mikrobiologii zgodnie z porozumieniem UMO-2018/31/D/NZ6/02839 z dnia 2019-07-01
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Two-dimensional vertical analysis of dam-break flow
PublikacjaW artykule przedstawiono matematyczne modelowanie przepływów ze swobodną powierzchnią w kanałach otwartych. Do symulacji przepływu użyto dwuwymiarowy pionowy model RANS. Wchodzące w skład modelu równania Naviera-Stokesa rozwiązano stosując algorytm SIMPLE dla metody różnic skończonych. Do śledzenia zmian swobodnego zwierciadła wody zastosowano metodę MAC. Analizie poddano szybkozmienny przepływ po poziomym, gładkim dnie wywołany...
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OFDM modulation and adaptive equalisation for underwater communications.
PublikacjaWłasności transmisyjne płytkiego kanału podwodnego są ograniczone ze względu na wielokrotne odbicia fal dźwiękowych od dna i powierzchni wody oraz ze względu na niestacionarność powodowaną głównie przez ruch powierzchni wody. W celu minimalizacji wpływu interferencji międzysymbolowych, powodowanych przez wielodrogową propagację fal dźwiękowych, w tradycyjnych wąskopasmowych modemach telekomunikacyjnych stosowane są złożone equalizery...
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Induction of apoptosis by triazoloacridinone derivative - C-1533 in HL60 and MOLT4 human leukemia cells.
PublikacjaPrzeprowadzone badania miały na celu określenie zdolności triazoloakrydonu C-1533 do indukcji śmierci komórek nowotworowych MOLT4 i HL60 na drodze apoptozy. Badanie zjawiska apoptozy przeprowadzono z wykorzystaniem techniki cytofluorymetrii przepływowej, mikroskopii fluorescencyjnej i elektroforezy agarozowej. Badania wykazały, że zarówno w komórkach MOLT4 jak i HL60, spadek mitochondrialnego potencjału błonowego oraz translokacja...
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Virtual Engineering Objects (VEO): Designing, Developing and Testing Models
PublikacjaIn this article, the development and implementation of the concept of Virtual Engineering Object (VEO) is described. A VEO is a computerized real world representation of an engineering object. VEO will act as a living representation of the object capable of adding, storing, improving and sharing knowledge through experience, in a way similar to an expert of that object. In this paper, it is shown through test models how the concept...
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Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor
PublikacjaEo-E RNA polymerase transcribes a regulon of folding factors for the bacterial envelope and is induced by physical and chemical stresses. The RseA anti-sigma factor inhibits the activity of Esigma(E) RNA, polymerase. It is shown here that the N-terminal portion of sigma(E), residues 1-153, binds core RNA polymerase. RseA interacts with residues 154-191 of sigma(E), a site that is homologous to region 4, the sigma factor binding...
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Dissociative multi-photon ionization of isolated uracil and uracil-adenine complexes
PublikacjaRecent multi-photon ionization (MPI) experiments on uracil revealed a fragment ion at m/z 84 that was proposed as a potential marker for ring opening in the electronically excited neutral molecule. The present MPI measurements on deuterated uracil identify the fragment as C3H4N2O+ (uracil+ less CO), a plausible dissociative ionization product from the theoretically predicted open-ring isomer. Equivalent measurements on thymine...
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A new assay based on terminal restriction fragment length polymorphism of homocitrate synthase gene fragments for Candida species identification
PublikacjaCandida sp. have been responsible for an increasing number of infections, especially in patients with immunodeficiency. Species specific differentiation of the Candida sp. is difficult in routine diagnosis. This identification can have a highly significant association in therapy and prophylaxis. This work has shown a new application of terminal restriction fragment length polymorphism (t-RFLP) method in molecular identification...
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Synthesis and biological evaluation of novel analogues of batracylin with synthetic amino acids and adenosine: an unexpected effect on centromere segregation in tumor cells through a dual inhibition of topoisomerase IIa and Aurora B
PublikacjaIn the search for new anticancer agents we designed and synthesized batracylin derivatives with linking synthetic amino acid side chains of different lengths and adenosine. Unexpectedly, we have found that in water and the culture media adenosine–amino acid–BAT conjugates form supramolecular structures and this prevents these compounds from entering cells. Consequently, these compounds exerted no biological activity when tested...
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Fungal Typin Methods
PublikacjaThe broad application of the molecular techniques in mycoses diagnosis is related to increase of infections caused by fungi in many countries. The oldest typing methods relaying on fenotypic observation, physiological and biochemical examination have had very limited importance from decades. Novadays, the molecular biology methods took their place. Most of the genotyping methods have been devoleped to be applied for typing of bacteria...
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New method for personalization of avatar animation
PublikacjaThe paper presents a method for creating a personalized animation of avatar utilizing fuzzy inference. First the user designs a prototype version of animation, with keyframes only for important poses, roughly describing the action. Then animation is enriched with new motion phases calculated by the fuzzy inference system using descriptors given by the user. Various degrees of motion fluency and naturalness are possible to achieve....
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Nowe rozwiązania metodyczne i techniczne w zakresie techniki mikroekstrakcji do fazy stracjonarnej
PublikacjaSample preparation has been recognized as a critical step of the analytical process, being even considered as the bottleneck of the overall process. Enrichment of target compounds, transfer of the analytes into a solvent compatible with the analytical instrumentation, minimization of potential interferences, and efficient sample clean-up, are among the main aims of sample preparation techniques. In this regard, liquid-liquid extraction...
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Spatial and vertical distribution analysis of heavy metals in urban retention tanks sediments: a case study of Strzyza Stream
PublikacjaConcentrations of seven heavy metals (HMs): Zn, Cu, Pb, Cd, Ni, Cr, and Fe in core samples of bottom sediments from four retention tanks (RTs) located along the Strzyza Stream in northern Poland (Gdansk) were measured to obtain a general view of sediment quality. The recognition of contamination and potential environmental impacts is the main aim of this paper. A total of 96 sediment samples were collected from eight sampling sites...
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Toward the next generation of air quality monitoring: Mercury
PublikacjaMercury is a global pollutant that is ubiquitous in the environment. Enrichment of mercury in the biosphere as the result of human activities and subsequent production of methylmercury (MeHg) has resulted in elevated concentrations in fish, wildlife and marine mammals globally. Elemental mercury (Hg0) is the most common form of mercury in the atmosphere, and the form that is most readily transported long distances from its emission...
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9,10-Dioxoanthracenyldithiocarbamates effectively inhibit the proliferation of non-small cell lung cancer by targeting multiple protein tyrosine kinases
PublikacjaAnthraquinones have attracted considerable interest in the realm of cancer treatment owing to their potent anticancer properties. This study evaluates the potential of a series of new anthraquinone derivatives as anticancer agents for non-small-cell lung cancer (NSCLC). The compounds were subjected to a range of tests to assess their cytotoxic and apoptotic properties, ability to inhibit colony formation, pro-DNA damage functions,...
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Novel primosomal protein B from thermophilic bacterium Thermoanerobacter tengcongensis
PublikacjaWe report the identification and characterization of theprimosomal protein B (PriB) from thermophilic bacteriumThermoanerobacter tengcongensis (TtePriB). It is the largestknown bacterial PriB protein consisting 216 amino acidresidues with a calculated molecular mass of 25 kDa. Surprisingly,it is functional as monomer containing two single-stranded DNA binding domain (OB-fold) and it is thecompletely new kind structure of SSB protein....
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Photophysics of Ru(II) Dyads Derived from Pyrenyl-Substitued Imidazo[4,5-f][1,10]phenanthroline Ligands.
PublikacjaThe photophysics of a series of Ru(II) dyads based on the 2-(1-pyrenyl)-1H-imidazo[4,5-f][1,10]-phenanthroline ligand was investigated. The ability of these metal complexes to intercalate DNA and induce cell death upon photoactivation makes them attractive photosensitizers for a range of photobiological applications, including photodynamic therapy. In the present study, time-resolved transient absorption and emission spectroscopy...
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Wykorzystanie metod akustycznych do monitoringu środowiska morskiego
PublikacjaW artykule przedstawiono metody i urządzenia wykorzystywane podczas obserwacji środowiska morskiego. Celem artykułu jest pokazanie możliwości odwzorowania środowiska morskiego w zależności od typu urządzenia, jego zasady działania i parametrów technicznych. Pokazane zostaną sposoby zobrazowania podwodnego za pomocą stosowanych obecnie w hydroakustyce urządzeń. Artykuł zawiera opis najczęściej stosowanych systemów wykorzystujących...
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The influence of global climate change on the environmental fate of anthropogenic pollution released from the permafrost
PublikacjaThis article presents a review of information related to the influence of potential permafrost degradation on the environmental fate of chemical species which are relased and stored, classified as potential influence in future Antarctic environment. Considering all data regarding climate change prediction, this topic may prove important issue for the future state of the Antarctic environment. A detailed survey on soil and permafrost...
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Implementing Smart Virtual Product Development (SVPD) to Support Product Manufacturing
PublikacjaThis paper illustrates the concept of providing the manufacturing knowledge during early stages of product life cycle to experts working on product development. The aim of this research is to enable a more collaborative product development environment by using Smart Virtual Product Development (SVPD) system, which is powered by Set of Experience Knowledge Structure (SOEKS) and Decisional DNA (DDNA). It enhances the industrial product...
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Likwidacja suchego doku przy Nabrzeżu Przemysłowym w Porcie Gdańsk
PublikacjaW niniejszej pracy przedstawiono problemy techniczne związane z likwidacją dużego obiektu hydrotechnicznego, stanowiącego utrudnienie nawigacyjne w intensywnie eksploatowanym portowym kanale dostępowym w Porcie Gdańsk. Dwukomorowy dok zlokalizowany przy Nabrzeżu Przemysłowym wybudowano w latach 1970-1972. Komory dokowe służyły do budowy żelbetowych, pływających skrzyń falochronowych, na potrzeby budowanego w tym czasie Portu Północnego....
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Stardust - Investigation of Microbes in the Stratosphere
PublikacjaThe stratospheric microbiome has been investigated several times using the methods of classical microbiology. In this experiment, we have combined them with some novel approaches including whole- metagenome amplification, Maldi TOF mass spectrometry and Sanger DNA sequencing. The results of the experiment may provide the scientists with knowledge about the mechanisms of survivability of microorganisms in stratospheric conditions...
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Current status and future success of renewable energy in Pakistan
PublikacjaMismatch between energy demand and supply from last two decades has been increasing because of the domination of expensive imported oil in energy mix of Pakistan. To import crude oil Government paid US $ 9 billion in 2008–2009 to meet the energy demands...
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The influence of sterilization with EnbioJet Microwave Flow Pasteurizer on composition and bioactivity of aronia and blue-berried honeysuckle juices
PublikacjaIn this study, fruit juices that are rich sources of anthocyanins, obtained from aronia (Am. chokeberry, Aronia melanocarpa) and blueberry honeysuckle (Lonicera caerulea L. var. edulis) were used to examine the preservation of plant phytochemicals and bioactivity upon sterilization -either thermal, or with an EnbioJet microwave flow pasteurizer. The chemical properties verified included determinations of anthocyanins and other...
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Cancer stem cells and escape from drug-induced premature senescence in human lung tumor cells: implications for drug resistance and in vitro drug screening models
PublikacjaBadania z użyciem modelu komórek nowotworu płuc A549 wykazały, że komórki te po traktowaniu lekami uszkadzającymi DNA zatrzymują proliferację i wchodzą w stan starzenia komórkowego. Jednak post-inkubacja tych komórek prowadzi do powrotu do stanu proliferacji małej frakcji komórek. Wykazaliśy, że frakcja ta związana jest z obecnością komórek pierwotnych nowotworu w populacji komórek A549. Powrót do stanu proliferacji komóek A549...
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Optimized 5-hour multiplex PCR test for the detection of tinea unguium: performance in a routine PCR laboratory
PublikacjaWe recently reported the development of a 5-hour multiplex PCR test for the detection of tinea unguium and the optimization of this test by the inclusion of an inhibition control. Here we report the performance of this procedure as used in a routine clinical laboratory as compared to conventional microscopy and culture-based techniques performed in a mycology reference laboratory. We found in processing 109 samples that 22 (20.2%)...
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BADANIA POLARNE AKADEMII MORSKIEJ W GDYNI
PublikacjaW pracy omówiono tematykę badań prowadzonych przez pracowników Wyższej Szkoły Mors-kiej/Akademii Morskiej w Gdyni w wysokich szerokościach półkul północnej i południowej. W latach 1975-2015 pracownicy tej uczelni opublikowali łącznie 231 artykułów, komunikatów i sprawozdań oraz 14 pozycji książkowych o charakterze monograficznym dotyczących różnych aspektów badań polarnych. Wśród tych prac 142 pozycje dotyczyły Arktyki i 103 pozycje...
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Anharmonic Infrared Spectroscopy through the Fourier Transform of Time Correlation Function Formalism in ONETEP
PublikacjaDensity functional theory molecular dynamics (DFT-MD) provides an efficient framework for accurately computing several types of spectra. The major benefit of DFTMD approaches lies in the ability to naturally take into account the effects of temperature and anharmonicity, without having to introduce any ad hoc or a posteriori corrections. Consequently, computational spectroscopy based on DFT-MD approaches plays a pivotal role in...
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Genetic and Epigenetic Aspects of Atopic Dermatitis
PublikacjaAtopic dermatitis is a heterogeneous disease, in which the pathogenesis is associated with mutations in genes encoding epidermal structural proteins, barrier enzymes, and their inhibitors; the role of genes regulating innate and adaptive immune responses and environmental factors inducing the disease is also noted. Recent studies point to the key role of epigenetic changes in the development of the disease. Epigenetic modifications...
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Knowledge-Based Virtual Modeling and Simulation of Manufacturing Processes for Industry 4.0
PublikacjaABSTRACT Industry 4.0 aims at providing a digital representation of a production landscape, but the challenges in building, maintaining, optimizing, and evolving digital models in inter-organizational production chains have not been identified yet in a systematic manner. In this paper, various Industry 4.0 research and technical challenges are addressed, and their present scenario is discussed. Moreover, in this article, the novel...