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Peptide Permeases in Candida albicans
PublicationAims: The aim of this work was to characterize oligopeptide uptake in Candida albicans mutants in which the genes encoding putative peptide permeases were selectively disrupted. Initial velocities of transport of model oligopeptides, (Ala)2, (Ala)3, (Ala)4, and sensitivity of mutants to antifungal oligopeptides containing N3-(4-methoxyfumaroyl)-L-2,3-diaminopropanoic acid (FMDP) were determined and compared to those of the wild-type...
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The use of plate springs for preloading of a system of tapered roller bearings of a wind turbine gearbox
PublicationThe use of preloaded tapered roller bearings in wind turbine drive systems allows a transfer of load in the case of high variations of axial forces. The examined bearing system, a modification of a current design, consists of a pair of different sized bearings. Previous study showed the high sensitivity of tapered roller bearings on the existing radial interference. Dimensional tolerances used in the original design do not allow...
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Public Perception of Digital Contact Tracing App and Implications for Technology Acceptance and Use Models
PublicationGovernments are increasingly adopting digital contact tracing applications (DCT) as a key component of their response to the COVID-19 pandemic. However, governments are struggling to achieve widespread adoption of DCT necessary for obtaining the expected individual and public benefits associated with its use. Consequently, studies on DCT have focused on the barriers to DCT adoption in different contexts and possible ways of overcoming...
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Non-invasive blood glucose monitoring with Raman spectroscopy: prospects for device miniaturization
PublicationThe number of patients with diabetes has reached over 350 million, and still continues to increase. The need for regular blood glucose monitoring sparks the interest in the development of modern detection technologies. One of those methods, which allows for noninvasive measurements, is Raman spectroscopy. The ability of infrared light to penetrate deep into tissues allows for obtaining measurements through the skin without its...
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Selection of Relevant Features for Text Classification with K-NN
PublicationIn this paper, we describe five features selection techniques used for a text classification. An information gain, independent significance feature test, chi-squared test, odds ratio test, and frequency filtering have been compared according to the text benchmarks based on Wikipedia. For each method we present the results of classification quality obtained on the test datasets using K-NN based approach. A main advantage of evaluated...
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PROPERTIES OF PARAMETRIC ALGORITHMIC DESIGN OF RESIDENTIAL HOUSES IN URBAN CONTEXT
PublicationDesign explorations of architectural avant-garde resulted in the development of digital techniques that allow solving very complex and demanding contemporary design challenges. In the architectural discourse the new design workshop seems to be symbolized mainly by the projects of sports facilities, cultural, administrative or multifunctional high-rise buildings. However for the quality of society life is more important to exploit...
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Release and Transport of Toxic, Mobile Organic Compounds (Formaldehyde and Phenols) on an Arctic Glacier
PublicationAs a result of current deglaciation, the chemical cycles of many compounds, including toxic formaldehyde and phenols, are changing. However, the processes by which these chemicals are released have yet to be studied in situ. Here, we quantify fluxes of HCHO and phenols in a glacial catchment within one summer season, obtaining a net release from the glacier of 0.106 · 106 g formaldehyde and 0.255 · 106 g phenols, which can be interpreted...
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KTaO3 a perovskite for water and air treatment
PublicationPerovskites are inorganic compounds that exhibit dielectric properties. These minerals belong to the crystal ceramics family. These compounds are described by the formula: ABX3 where, A and B are 2, 3 or 4 valuable metal ions, and X is an ion of oxygen or fluorine. An example of perovskite is the compound KTaO3. There are many methods for producing KTaO3, but the hydrothermal method is most commonly used. Perovskites can be characterized...
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Determination of the minimal critical rotational speeds of the circular saw blades with the quasi-twin resonant frequencies
PublicationTo determine the minimal critical rotational speeds of the circular saw blades is the fundamental aspect of obtaining the range of the rotational operating speeds, by which the circular saw blade can work with required stability. While for the circular saw blades with full-homogeneous bodies the determination of such rotational speeds is the relatively low level of difficulty function, whereas for circular saw blades with more...
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Green analytical chemistry : theory and practice
PublicationThis tutorial review summarises the current state of green analytical chemistry with special emphasis on environmentally friendly sample preparation techniques. Green analytical chemistry is a part of the sustainable development concept; its history and origins are described. Miniaturisation of analytical devices and shortening the time elapsing between performing analysis and obtaining reliable analytical results are important...
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Agent System for Managing Distributed Mobile Interactive Documents in Knowledge-Based Organizations
PublicationThe MIND architecture of distributed mobile interactive document is a new processing model defined to facilitate obtaining a proper solution in knowledge processes carried out by knowledge-based organizations. Such organizations have an established structure that defines document templates and knowledge process. The aim of the MIND architecture is to change the static document to mobile agents, which are designed to implement the...
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Exfoliated graphite with spinel oxide as an effective hybrid electrocatalyst for water splitting
PublicationThe aim of the conducted research was to develop hybrid nanostructures formed from MnCo2O4 and exfoliated graphite. Carbon added during the synthesis allowed for obtaining a well-distributed MnCo2O4 particle size with exposed active sites contributing to the increased electric conductivity. The influence of the weight ratios of carbon to a catalyst for hydrogen and oxygen evolution reactions was investigated. The new bifunctional...
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Selecting an Applicable Cybersecurity Assessment Framework: Qualitative Metrics-Based Multiple-Factor Analysis
PublicationRecently, a survey of cybersecurity assessment methods focused on general characteristics was conducted. Among its major findings, it revealed the methods’ adoption issues. This paper presents a follow-up to the study. It provides an in-depth analysis of the methods’ adoption-related properties based on qualitative metrics. As a result, the proposals which demonstrate a higher adoption potential were identified. The methods are...
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Diagnostyka analogowych filtrów wielosekcyjnych oparta na klasyfikato-rach neuronowych z dwucentrowymi funkcjami bazowymi
PublicationPrzedmiotem artykułu jest zastosowanie klasyfikatora z dwucentrowymi funkcjami bazowymi do lokalizacji uszkodzeń w wielosekcyjnych torach analogowych elektronicznych systemów wbudowanych sterowanych mikrokontrolerem. Przedstawiono szczegóły procedury pomiarowej oraz metody detekcji i lokalizacji uszkodzeń toru analogowego z wykorzysta-niem klasyfikatora DB zaimplementowanego w postaci algorytmicznej w kodzie programu mikrokontrolera....
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STRUKTURA SZKŁO-CERAMIKI ZAWIERAJĄCEJ FERROELEKTRYCZNĄ FAZĘ BiV
PublicationLiteratura opiusuje próby wytworzenia nanokompozytów, w których skład wchodzą matryca szkła i rozproszone w niej nanokrystality fazy ferroelektrycznej. Najczęstsza metoda wykorzystywana do otrzymywania takiej szkło - ceramiki polega na wytopie szkła, a następnie jego krystalizacji. W literaturze można znaleźć dużo przykładów wytwarzania tego typu materiałów dwu lub więcej składnikowych. Najwięcej badań dotyczy ferroelektrycznych...
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KORPUS MOWY ANGIELSKIEJ DO CELÓW MULTIMODALNEGO AUTOMATYCZNEGO ROZPOZNAWANIA MOWY
PublicationW referacie zaprezentowano audiowizualny korpus mowy zawierający 31 godzin nagrań mowy w języku angielskim. Korpus dedykowany jest do celów automatycznego audiowizualnego rozpoznawania mowy. Korpus zawiera nagrania wideo pochodzące z szybkoklatkowej kamery stereowizyjnej oraz dźwięk zarejestrowany przez matrycę mikrofonową i mikrofon komputera przenośnego. Dzięki uwzględnieniu nagrań zarejestrowanych w warunkach szumowych korpus...
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Distribution of relaxation times as a method of separation and identification of complex processes measured by impedance spectroscopy
PublicationImpedance spectroscopy is one of the most commonly performed measurements to characterize electronic and electrochemical systems. Impedance spectra have limited resolution and many different processes may overlap what could be the reason of obstructions in its proper later analysis. Up to date, there are three approaches to solve this problem: examining impedance spectra itself, fitting spectra with equivalent circuits, and calculating...
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Structural Assessment of Reinforced Concrete Beams Incorporating Waste Plastic Straws
PublicationThe behavior of reinforced concrete beams containing fibers made of waste plastic straws (WPSs) under the three point bending test is examined. The effect of WPS fiber addition on the compressive and split tensile strength is reported. Four concrete mixes were prepared. The control mix PS-0 had a proportion of 1 cement: 1 sand: 2 coarse aggregate and a water cement ratio of 0.4. In the other three mixes PS-0.5, PS-1.5 and PS-3,...
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Transport properties of aqueous ionic liquid microemulsions: influence of the anion type and presence of the cosurfactant
PublicationTransport properties, viz. specific conductivity, dynamic viscosity and apparent diffusion coefficients, were measured as a function of water content in aqueous ionic liquid microemulsions containing 1-butyl-3- methylimidazolium hexafluorophosphate, [BMIM][PF6] and bis(trifluoromethanesulphonyl)imide, [BMIM][Tf2N], stabilized by the nonionic surfactant TX-100, or its mixture with a cosurfactant, i.e. butanol. The investigation covered...
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Influence of Ionic Liquid Structure on Supported Ionic Liquid Membranes Effectiveness in Carbon Dioxide/Methane Separation
PublicationThis paper indicates the possibility of application of imidazolium ionic liquids immobilized in polymeric supports—supported ionic liquid membranes—in CO2 separation from gaseous streams (e.g., biogas). Imidazolium salts containing alkyl fluoride anions, bis(trifluoromethylsulfonyl)imide and trifluoromethanesulfonate, selectively separating CO2 were used. The permeability of CO2 through membranes was investigated under gas pressure...
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An Attempt to Create Speech Synthesis Model That Retains Lombard Effect Characteristics
PublicationThe speech with the Lombard effect has been extensively studied in the context of speech recognition or speech enhancement. However, few studies have investigated the Lombard effect in the context of speech synthesis. The aim of this paper is to create a mathematical model that allows for retaining the Lombard effect. These models could be used as a basis of a formant speech synthesizer. The proposed models are based on dividing...
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Properties of LiMnBO3 glasses and nanostructured glass-ceramics
PublicationPolycrystalline LiMnBO3 is a promising cathode material for Li-ion batteries. In this work, we investigated the thermal, structural and electrical properties of glassy and nanocrystallized materials having the same chemical composition. The original glass was obtained via a standard melt-quenching method. SEM and 7Li solid-state NMR indicate that it contains a mixture of two distinct glassy phases. The results suggest that the...
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Improving carbon dioxide capture in aqueous ammonia solutions by fine SiO2 particles
PublicationAmmonia solutions are considered to be effective solvents for carbon dioxide absorption. Despite the numerous advantages of these solvents, their high volatility is a significant technical and economic problem. Therefore, in this work, silica particles were used as additives to improve CO2 absorption and inhibit NH3 desorption. SiO2 microparticles and colloidal SiO2 particles in the concentration range of 0-0.15 wt.% were used...
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The Impact of Thermal Stresses on Volume Resistivity: Performance Comparison between TR-XLPE and XLPE Cables
PublicationMost failures in electric power cables are attributed to the development of water trees inside the insulating materials. Thus, the development and utilization of Tree Retardant Cross-Linked Polyethylene (TR-XLPE) based cables have recently been given great attention to overcome the treeing issues. TR-XLPE cables use low-density polyethylene compounds suppressing the formation of water trees while retaining excellent electrical,...
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Effect of TiO2 Concentration on Microstructure and Properties of Composite Cu–Sn–TiO2 Coatings Obtained by Electrodeposition
PublicationIn this work, Cu–Sn–TiO2 composite coatings were electrochemically obtained from a sulfate bath containing 0–10 g/L of TiO2 nanoparticles. The effect of TiO2 particles on kinetics of cathodic electrodeposition has been studied by linear sweep voltammetry and chronopotentiometry. As compared to the Cu–Sn alloy, the Cu–Sn–TiO2 composite coatings show rougher surfaces with TiO2 agglomerates embedded in the metal matrix. The highest...
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Modyfikacja cementu kostnego w celu uzyskania długotrwałej ochrony antybakteryjnej
PublicationNiniejsza rozprawa doktorska stanowi przewodnik po jednotematycznym cyklu publikacji, dotyczącym modyfikacji akrylowego cementu kostnego celem uzyskania długotrwałej i efektywnej ochrony antybakteryjnej. W ramach niego przeprowadzono badania eksperymentalne polegające na opracowaniu technologii wytwarzania modyfikowanego cementu kostnego oraz procedury medycznej jego stosowania. Cement kostny był modyfikowany przez zastosowanie...
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Transport deficiency is the molecular basis of Candida albicans resistance to antifungal oligopeptides
Publication(FMDP), an inhibitor of glucosamine-6-phosphate synthase, exhibited growth inhibitory activity against Candida albicans, with minimal inhibitory concentration values in the 0.05–50 mg/L range. Uptake by the peptide permeases was found to be the main factor limiting an anticandidal activity of these compounds. Di- and tripeptide containing FMDP (F2 and F3) were transported by Ptr2p/Ptr22p peptide transporters (PTR) and FMDP-containing...
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Experimental Study of the Resistance to Influence of Aggressive Liquids on Lightweight Concrete
PublicationIn light of the scientific research, the corrosion of concrete structures is one of the main problems that may reduce their durability due to the negative impact of the natural environment. The paper analyzes the influence of the type of component on the selected properties of lightweight concrete subjected to the influence of aggressive liquids. Four concrete mixes were prepared with a granular aggregate made of foamed glass (GEGA)...
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Surface modifications of ti and its alloys
PublicationThis article reviews the various surface modification techniques pertaining to titanium and titanium alloys including physical treatment, mechanical treatment, and chemical and electrochemical treatment. The proper surface modification expands the use of titanium and its alloys in the biomedical field for long-term implants retaining the excellent properties of substrate material and improving the specific surface properties required...
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Preparation of Pseudopeptides Building Blocks with Retro-Thioamide Bond Mediated via Thiocarbamoyl Meldrum's Acid
PublicationAn easy and efficient synthesis of pseudo tripeptide containing a thiomalonamide moiety was developed. Isothiocyanate derivatives of amino acids react smoothly with 2,2-dimethyl-1,3-dioxane-4,6-dione yielding new thiocarbamoyl Meldrum's acids. Thermal decomposition of this new Meldrum's acid derivatives generate thiocarbamoyl ketenes, which acylate amino acid esters to give pseudo tripeptides.
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Video of LEGO Bricks on Conveyor Belt Dataset Series
PublicationThe dataset series titled Video of LEGO bricks on conveyor belt is composed of 14 datasets containing video recordings of a moving white conveyor belt. The recordings were created using a smartphone camera in Full HD resolution. The dataset allows for the preparation of data for neural network training, and building of a LEGO sorting machine that can help builders to organise their collections.
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Discrete Modelling of Micro-structural Phenomena in Granular Shear Zones
PublicationThe micro-structure evolution in shear zones in cohesionless sand for quasi-static problems was analyzed with a discrete element method (DEM). The passive sand failure for a very rought retaining wall undergoing horizontal translation towards the sand backfill was discussed. To simulate the behaviour of sand, the spherical discrete element model was used with elements in the form of rigid spheres with contacts moments.
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Synthesis and crystal structure of ferrocenyl benzothiazole derivatives
PublicationNovel ferrocenyl-containing benzothiazole derivatives were synthesised in moderate yields by one-pot α-amidoalkylation of ferrocene. The reaction conditions were critically evaluated and the crystal structure of one of the amidoalkylated ferrocenes was resolved. Oxidation of the obtained compounds led to known fully aromatic 2-ferrocenylbenzothiazole in 81 % yield, which represents a convenient alternative to earlier methods utilizing...
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Prospects of ionic liquids application in electronic and bioelectronic nose instruments
PublicationThis paper addresses the problem of application of ionic liquids to chemical sensors and biosensors being an integral part of the electronic and bioelectronic-type instruments. The design and principle of operation of the electronic and bioelectronic noses are compared. Both the benefits and shortcomings of the application of ionic liquids in these type of instruments are described. The prospects of the development and application...
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Polyethylene glycol-coated solid-phase microextraction fibres for the extraction of polar analytes-A review
PublicationThe article discusses the merits and limitations of commercially available solid-phase microextraction (SPME) fibres and compares them with the new type of extraction coatings, in particular those containing polyethylene glycol as sorbent, as well as the methods of the preparation of the latter. It also analyses their possible application for the extraction of a broad spectrum of analytes, with particular emphasis on the sampling...
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Paid work activity and entrepreneurial cognitions of students – evidence from European emerging economies
PublicationPurpose – This paper aims to investigate whether paid work activity (PWA) experience of students from five emerging economies is related to academic results and self-assessment of possessed entrepreneurial traits. Additionally, the authors verify the relationship between obtaining work experience and the willingness to start own business among students. Design/methodology/approach – Participants included 3,631 students of the first...
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Review on friction stir welding of dissimilar magnesium and aluminum alloys: Scientometric analysis and strategies for achieving high-quality joints
PublicationMagnesium and aluminum alloys continually attract interest as lightweight structural materials for transport applications. However, joining these dissimilar alloys is very challenging. The main obstacle that hinders progress in dissimilar Mg-Al joining is the formation of brittle intermetallic compounds (IMCs). As a solid-state joining technique, FSW is an excellent candidate to attenuate the deleterious IMC effects in dissimilar...
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Zastosowanie oprogramowania COMSIS do oceny dynamicznej odporności na zakłócenia cyfrowych układów kombinacyjnych.
PublicationWyniki badania odporności układów cyfrowych na zakłócenie dynamiczne w istotny sposób zależą od parametrów sygnału stymulującego. Proponowana metodyka zakłada pobudzanie badanego układu sygnałem szumu białego o określonej szerokości pasma częstotliwości. Badania symulacyjne z modelem układu progowego oraz szybkiego komparatora z histerezą przeprowadzono w środowisku COMSIS. Uzyskane wyniki potwierdzają, że im węższe pasmo szumu...
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Tworzenie modelu pojęciowego dla zarządzania ryzykiem na przykładzie procesu laminowania w stoczni jachtowej
PublicationCelem artykułu jest zaproponowanie modelu pojęciowego normalizującego kryteria oceny ryzyka w systemie zarządzania jakością w stoczni jachtowej. Zamiarem jest przeprowadzenie analizy danych, pozwalającej wytyczyć zbiory o wysokim, średnim i niskim poziomie istotności dla wszystkich błędów powstających w procesie laminowania. W artykule skoncentrowano się na najważniejszych pojęciach związanych z ryzykiem i kryteriami jego oceny....
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Induction of immune responses in sheep by vaccination with liposome-entrapped DNA complexes encoding Toxoplasma gondii MIC3 gene
PublicationToxoplasma gondii is a parasite that has been extensively studied due to its medical and veterinary importance in terminating pregnancies. Consequently, a satisfactory vaccine is required to control itsadverse effects on pregnant animals. The microneme protein, MIC3, is a major adhesion protein that binds to the surface of host cells and parasites, and is therefore a potential vaccine against T. gondii.The viability of MIC3 as...
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Expression of Deinococcus geothermalis Trehalose Synthase Gene in Escherichia coli and Its Enzymatic Properties
PublicationA novel trehalose synthase gene from Deinococcus geothermalis (DSMZ 11300) containing 1,692 bp reading-frame encoding 564 amino acids was amplified using PCR. The gene was ligated into pET30Ek/LIC vector and expressed after isopropyl alfa-D-thiogalactopyranoside induction in Escherichia coli BL21(DE3)pLysS. The recombinant trehalose synthase (DgeoTreS) containing a His6 tag at the C-terminus was purified by metal affinity chromatography...
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Preparation and evaluation of 1,3-alternate 25,27-bis-(pentafluorobenzyloxy)-26,28-bis-(3-propyloxy)-calix[4]arene-bonded silics gel high performance liquid chromatography stationary phase
PublicationA 1,3-alternate 25,27-bis-(pentafluorobenzyloxy)-26,28-bis-(3-propyloxy)-calix[4]arene-bonded silica gel stationary phase (CalixBzF10) was synthesized, structurally characterized, and used as a selector in liquid chromatography. The selectivity study of this phase was done by using fluorine-containing compounds (fluorobenzenes, fluoro-pyrimidine bases), as well as non-fluorinated analytes (non-steroidal anti-inflammatory drugs,...
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Peptide conjugates of lactoferricin analogues and antimicrobials—Design, chemical synthesis, evaluation of antimicrobial activity and mammalian cytotoxicity
PublicationEight new peptide conjugates composed of modified bovine lactoferricin truncated analogues (LFcinB) and oneof the three antimicrobials — ciprofloxacin (CIP), levofloxacin (LVX), and fluconazole (FLC) — were synthesized. Four different linkers were applied to connect a peptide and an antimicrobial agent. The FLC-containing peptidic conjugates were synthesized using the “click chemistry” method. This novel approach is reported here...
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Characterization of GM3 Gangliosides in Human Milk throughout Lactation: Insights from the Analysis with the Use of Reversed-Phase Liquid Chromatography Coupled to Quadrupole Time-Of-Flight Mass Spectrometry
PublicationGangliosides are complex lipids found in human milk that play important structural and biological functions. In this study, we utilized reversed-phase liquid chromatography coupled to quadrupole time-of-flight mass spectrometry to evaluate the molecular distribution of GM3 in human milk samples collected at distinct lactation stages, ranging from colostrum to advanced lactation samples. Throughout lactation, GM3 d40:1 emerged as...
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Resonant Frequencies in Microstrip Structure with Omega Medium Substrate
PublicationThe paper presents the research on a rectangular microstrip structure with multilayer substrate containing dielectric and omega medium layers. The effect of pseudochiral medium layer location in the substrate and its thickness on the resonant frequency of the rectangular microstrip structure is investigated. The numerical analysis of investigated structures is based on expansion of electric and magnetic fields. Utilizing the continuity...
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Peptide modification with thiomalonoamide moiety
PublicationAbstract: Meldrum acid derivatives under the thermal decomposition are well known source of ketenes. Recently we investigated chemical properties of thiocarbamolyl meldrum acids. We found that 5-(α-alkylamino- α'-sulfhydryl)methylene Meldrum's acids hated in the presence of amines produce thiomalonamides. We used this reaction to the one pot synthesis of series of short peptides containing thiomalonoamide motif.
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Overview of the Synthesis and Catalytic Reactivity of Transition Metal Complexes Based on C═P Bond Systems
PublicationThis report provides an overview of the use of phosphaalkenes (C=P) in syntheses of various complexes. Going through successive complexes containing the d-block metals, we sought how this area of chemistry was formed over the years. Additionally, this work contains information on the possible coordination types and means of constructing the complexes. In addition, this review reveals the reactivities of phosphaalkene complexes...
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Akustyczna analiza natężenia ruchu drogowego dla systemów zarządzania ruchem
PublicationW pracy przybliżono wybrane zagadnienia z dziedziny zarządzania transportem drogowym w Polsce i na świecie. W tym kontekście pzredstawiono potrzeby rynkowe, wymagania jak i możliwości w zakresie pozyskiwania informacji o aktualnym stanie sieci drogowych. Zaproponowano akustyczną metodę nadzorowania ruchu drogowego i jej możliwości w kontekście systemów zarządzania ruchem. Przedstawiono schemat akwizycji sygnału wraz z danymi odniesienia....
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Temperature Sensors Based on Polymer Fiber Optic Interferometer
PublicationTemperature measurements are of great importance in many fields of human activities, including industry, technology, and science. For example, obtaining a certain temperature value or a sudden change in it can be the primary control marker of a chemical process. Fiber optic sensors have remarkable properties giving a broad range of applications. They enable continuous real-time temperature control in difficult-to-reach areas, in...
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Automatic Clustering of EEG-Based Data Associated with Brain Activity
PublicationThe aim of this paper is to present a system for automatic assigning electroencephalographic (EEG) signals to appropriate classes associated with brain activity. The EEG signals are acquired from a headset consisting of 14 electrodes placed on skull. Data gathered are first processed by the Independent Component Analysis algorithm to obtain estimates of signals generated by primary sources reflecting the activity of the brain....