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Search results for: BAKTERIE COLI
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Colonization of axenic beet by rhizosphere bacteria takes place in discreet phases regardless of bioinculation with next generation bioinoculant
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Development of Safirinium dyes for new applications: fluorescent staining of bacteria, human kidney cells, and the horny layer of the epidermis
PublicationLow-molecular synthetic fluorophores are convenient tools in bioimaging applications. Several derivatives of Safirinium dyes as well as their reactive N-hydroxysuccinimide (NHS) esters bearing diverse substituents were synthesized and evaluated experimentally in terms of their lipophilicity by means of reverse-phase and immobilized artificial membrane high-performance liquid chromatography. Subsequently, the selected compounds...
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The valance state of vanadium-key factor in the flexibility of potassium vanadates structure as cathode materials in Li-ion batteries
PublicationPotassium hexavanadate (K2V6O16·nH2O) nanobelts have been synthesized by the LPE-IonEx method, which is dedicated to synthesis of transition metal oxide bronzes with controlled morphology and structure. The electrochemical performance of K2V6O16·nH2O as a cathode material for lithium-ion batteries has been evaluated. The KVO nanobelts demonstrated a high discharge capacity of 260 mAh g−1, and long-term cyclic stability up to 100...
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Closed-Loop Control System Design for Wireless Charging of Low-Voltage EV Batteries with Time-Delay Constraints
PublicationThis paper presents an inductive power transfer system on the basis of a double single- phase three-level T-type inverter and two split transmitting coils for constant current and constant voltage wireless charging of low-voltage light electric vehicle batteries with closed-loop control, considering time-delay communication constraints. An optimal control structure and a modified control strategy were chosen and implemented to...
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The Antimicrobial Potential of Bacteria Isolated from Honey Samples Produced in the Apiaries Located in Pomeranian Voivodeship in Northern Poland
PublicationThe principal objective of this study was to determine whether the honeys produced in apiaries located in Pomeranian Voivodeship (Northern Poland) contain bacteria producing metabolites with growth inhibition potential against important human and animal pathogens. The pathogens included Staphylococcus aurues, Staphyloccocus epidermidis, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, and Candida albicans. From...
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Correlation between chemical composition and the presence of selected groups of bacteria in freshwater samples collected from Isfjorden and Billefjorde
PublicationThe average concentrations of pollutants in the arctic water, snow and the atmosphere are much lower than those observed in the temperate climate. Specific conditions occurring in the polar regions have apotential to accumulate the pollutants transported from other parts of the world. In this study, attempts were made to find a correlation between selected chemical components and the bacterial population. The analysis involved...
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Comparison of antimicrobial activity of selected, commercially available wound dressing materials
PublicationOBJECTIVE: The aim of our study was to examine the antimicrobial potential of eight selected, commercially available wound dressings containing different antimicrobial agents: silver, chlorhexidine acetate, povidone-iodine, and manuka honey. METHOD: The materials were tested against four reference strains of bacteria: Staphylococcus aureus (PCM 2051), Staphylococcus epidermidis (PCM 2118), Pseudomonas aeruginosa (ATCC 27853),...
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WYKORZYSTANIE BATERII KONDENSATORÓW ZAINSTALOWANYCH W SIECI SN DO OGRANICZENIA START MOCY W SIECI
PublicationW artykule przedstawiono wyniki badań pokazujące możliwości wykorzystania baterii kondensatorów zainstalowanych wewnątrz sieci SN (w odbiorców przemysłowych), w celu zminimalizowania strat mocy w sieci elektroenergetycznej. Wyniki analizy pozwoliły określić, które ze stosowanych obecnie kryteriów regulacji jest najbardziej korzystne. Wskazywano również na konieczność koordynacji działań regulatorów transformatora i baterii kondensatorów.
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Structure of EstA esterase from psychrotrophic Pseudoalteromonas sp. 643A covalently inhibited by monoethylphosphonate.
PublicationThe crystal structure of the esterase EstA from the cold-adapted bacteriumPseudoalteromonas sp. 643A was determined in a covalently inhibited form at aresolution of 1.35 A˚. The enzyme has a typical SGNH hydrolase structureconsisting of a single domain containing a five-stranded beta-sheet, with threehelices at the convex side and two helices at the concave side of the sheet, and isornamented with a couple of very short helices...
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Bacterial SSB-like proteins of family Deinococcaceae - indentification, isolation, obtaining, purification and molecular characteristic
PublicationWiększość poznanych białek SSB jest aktywna w formie homotetramerycznej. Od 2002 roku znane są białka organizmów ekstremofilnych. Scharakteryzowano białka mikroorganizmów rodziny Deinococaceae. Uzyskane termostabilne białka stanowią atrakcyjną alternatywę w stosunku do znanych białek SSB w metodach diagnostyki molekularnej, technikach biologii molekularnej i analityce.
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Analyzing and improving a mechanistic model for the Microthrix parvicella in activated sludge systems
PublicationW pracy przedstawiono wyniki estymacji parametrów dla mechanistycznego modelu bakterii Microthrix parvicella w systemach osadu czynnego. W tym celu wykorzystano dane z oczyszczalni ścieków w Szwajcarii oraz wyniki dodatkowych badań laboratoryjnych w reaktorze nieprzepływowym. Model właściwie prognozował szybkość poboru tlenu (mierzoną dla różnych substratów) oraz zawartść bakterii Microthrix w biomasie osadu czynnego.
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Changes in bacterial cells induced by high pressure at subzero temperature
PublicationW publikacji przedstawiono wyniki badań dotyczące zmian jakie zachodzą w komórkach bakterii Escherichia coli po działaniu wysokiego ciśnienia w ujemnej temperaturze. Stwierdzono, że w wyniku działania ciśnienia 193 MPa zachodzącą zmiany w błonie cytoplazmatycznej i ścianie komórkowej bakterii. W tych warunkach następowała prawie całkowita utrata wewnątrzkomórkowego ATP z komórek badanych szczepów znajdujących się w fazie logarytmicznej....
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Lipidomic characteristics of three edible cold-pressed oils by LC/Q-TOF for simple quality and authenticity assurance
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Discrimination of Selected Cold-Pressed and Refined Oils by Untargeted Profiling of Phase Transition Curves of Differential Scanning Calorimetry
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A new cold-active β-galactosidase from Arthrobacter sp. S3* - gene cloning, overexpression, purification and properties
PublicationA psychrotrophic bacterium producing a cold-active β-galactosidase was isolated from Spitsbergen soil and classified as Arthrobacter sp. S3*. The gene encoding β-galactosidase was isolated from the genomic DNA library, sequenced, cloned, expressed in Escherichia coli, purified by ion exchange chromatography and characterized. The Arthrobaster sp. S3* β-galactosidase is a homotrimeric enzyme composed of 74,4 kDa subunits. It is...
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Physico‐chemical and proteolytic changes during cold storage of dry‐cured pork loins with probiotic strains ofLAB
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Changes in chemical composition and oxidative stability of cold-pressed oils obtained from by-product roasted berry seeds
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An Investigation into Cold Start Emissions from Compression Ignition Engines using EU Legislative Emissions Test Procedures
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Synteza i właściwości warstw poli(3,4-etylenodioksytiofenu) modyfikowanego heksacyjanożelazianami (II/III) wybranych metali przejściowych
PublicationPraca dotyczy modyfikacji warstw polimeru elektroatywnego poli(3,4-etylenodioksytiofenu) heksacyjanożelazianami (II/III) wybranych metali przejściowych (Fe, Co, Ni).
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Purification and biochemical characteristic of a cold-active recombinant esterase from Pseudoalteromonas sp. 643A under denaturing conditions.
PublicationIn this paper production of a cold-active esterase EstA from the Antarctic bacterium Pseudoalteromonas sp. 643A in E. coli expression system was described. The purification and biochemical characteristic of EstA were performed in the presence of urea and then compared with results obtained for the esterase with no addition of urea and isolated from the native source. In both cases the cold-active enzyme displayed similar properties....
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Plant Extracts Activated by Cold Atmospheric Pressure Plasmas as Suitable Tools for Synthesis of Gold Nanostructures with Catalytic Uses
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A novel cold-active beta-D-galactosidase from the Paracoccus sp. 32d - gene cloning, purification and characterization
PublicationBeta-D-galactosidase (EC 3.2.1.23) catalyze the hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Cold-active beta-D-galactosidases have recently become a focus of attention of researchers and dairy product manufactures owing to theirs ability to: (I) eliminate of lactose from refrigerated milk for people afflicted with lactose intolerance, (II) convert lactose to glucose and galactose which...
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Virgin rapeseed oils obtained from different rape varieties by cold pressed method - their characteristics, properties, and differences
PublicationCelem pracy było porównanie olejów uzyskanych z różnych odmian rzepaku. Oznaczono skład kwasów tłuszczowych, wartości liczb charakterystycznych (AcV, AnV, PV, TOTOX), zawartość karotenów, tokoferoli, chlorofili, polifenoli, metali (żelazo i miedź). Oznaczono stabilność oksydatywną olejów tłoczonych na zimno. Oznaczono skład frakcji lotnej i przeprowadzono analizy sensoryczne.
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Cloning, expression, and purification of a recombinant cold-adapted β-galactosidase from antarctic bacterium Pseudoalteromonas sp. 22b
PublicationAntarktyczny szczep, gram-ujemnych bakterii Pseudoalteromonas sp. 22b, wyizolowanych z przewodu pokarmowego kryla z gatunku Thyssanoessa macrura, jest producentem wewnątrzkomórkowej zimnolubnej beta-galaktozydazy. Białko to jest homotetramerem, w którym każda z jednostek monomeru zbudowana jest z 1028 reszt aminokwasowych. Gen kodujący ten enzym został sklonowany i eksprymowany w komórkach E. coli. Następnie z zastosowaniem procedury...
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TS-based RSM-aided design of cold-formed steel stiffened C-sectional columns susceptible to buckling
PublicationThe paper focuses on joint-performance analysis of a built-up cold-formed thin-walled C-sectional column with a fillister (stiffener) of the same type, interconnected with a series of bolts located along the length of the compressed members in specific spacing. The quantity of bolts and the non-connected starting distance of the fillister are considered random variables of the problem. Optimal distribution of joints on the stiffener...
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Coupling of Blood Pressure and Subarachnoid Space Oscillations at Cardiac Frequency Evoked by Handgrip and Cold Tests: A Bispectral Analysis
PublicationThe aim of the study was to assess blood pressure–subarachnoid space (BP–SAS) width coupling properties using time–frequency bispectral analysis based on wavelet transforms during handgrip and cold tests. The experiments were performed on a group of 16 healthy subjects (F/M; 7/9) of the mean age 27.2 ± 6.8 years and body mass index of 23.8 ± 4.1 kg/m². The sequence of challenges was first handgrip and then cold test. The handgrip...
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Combined cold supply system for ship application based on low GWP refrigerants - Thermo-economic and ecological analyses
PublicationThe withdrawal of popular high-GWP refrigerants will significantly affect maritime industry. Refrigeration and air conditioning systems currently used on ships are not ready to fulfil future limitations. Therefore, there is a need for system, which will operate with low-GWP refrigerants and at the same time will be efficient and reliable in difficult environmental conditions. This paper explains the concept of a cold supply system,...
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New perspective on the in vivo use of cold stress dynamic thermography in integumental reconstruction with the use of skin-muscle flaps
PublicationAmong the problems encountered by plastic surgeons is the reconstruction of defects following tumors. One of the reconstructive options is TRAM flap. Despite that anatomy is well-explored, marginal flap necrosis may develop. To minimize complications imaging examinations was designed to determine the degree of flap perfusion. One of them is the thermographic examination.
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New ceramic materials derived from pyrolyzed poly(1,2-dimethylsilazane) and starch as a potential anode for Li-ion batteries
PublicationNewmaterialswere obtained by pyrolysis of starch (S) and poly(1,2-dimethylsilazane) (PSN) (weight ratio: PSN/S 30/70) at temperature a) 500 °C, b) 700 °C and c) 900 °C. Ceramic materials were characterized by infrared spectroscopy, TGA, Raman spectroscopy and SEM. New Si\O and shifted Si\C stretching vibration modes emerged confirming direct interaction between silicon originating fromsilazane and oxygen coming fromstarch. The...
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Application of Extreme Doses of Aluminium for Growth Control of Filmentous Bacteria Microthrix Parvicella in an Activated Sludge System - Laboratory Investigations
PublicationLaboratory investigations of aluminium (PAX-16) application for control of population of filamentous bacteria Microthrix Parvicella in a three-phase bioreactor in lab-scale were performed. Dosing of small aluminium doses (approximately 0.5 mgAl/gMLSS·d) in the period preceding seasonal growth of M.Parvicella failed to prevent mass growth of this microorganism leading to activated sludge bulking phenomenon (SVI-250 ml/gMLSS)...
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Flexible Films as Anode Materials Based on rGO and TiO2/MnO2 in Li-Ion Batteries Free of Non-Active Agents
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Photodynamic Inactivation of Bacteria with Porphyrin Derivatives: Effect of Charge, Lipophilicity, ROS Generation, and Cellular Uptake on Their Biological Activity In Vitro
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Molecular modeling of Gram-positive bacteria peptidoglycan layer, selected glycopeptide antibiotics and vancomycin derivatives modified with sugar moieties
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Tideless estuaries in brackish seas as possible freshwater‐marine transition zones for bacteria: the case study of the Vistula river estuary
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The coexistence and competition of canonical and comammox nitrite oxidizing bacteria in a nitrifying activated sludge system – Experimental observations and simulation studies
PublicationThe second step of nitrification can be mediated by nitrite oxidizing bacteria (NOB), i.e. Nitrospira and Nitrobacter, with different characteristics in terms of the r/K theory. In this study, an activated sludge model was developed to account for competition between two groups of canonical NOB and comammox bacteria. Heterotrophic denitrification on soluble microbial products was also incorporated into the model. Four 5-week washout...
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Evaluating the antibacterial activity of muramyl dipeptide derivatives, retro-tuftsin derivatives, and anthraquinone oligopeptides against a range of pathogenic bacteria
PublicationSearch for new and efficient antibiotic is crucial because of microbial drug resistance and problems with side effects of the administered medication. In this study, we evaluate the in vitro microbiological activity of muramyl dipeptide derivatives, retro-tuftsin derivatives (i.e., tuftsin with reversed amino acid sequences), and combinations of retro-tuftsin derivatives with substituted anthraquinones. The potency of the investigated...
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Organizmy obecne w osadzie dennym basenów kąpielowych
PublicationMonitorowanie parametrów wody basenowej jest istotne z uwagi na zdrowie osób korzystających z basenu jak i pracowników obsługi. Istnieją trzy źródła materii organicznej: woda dostarczona do basenu, pasywne zanieczyszczenia wnoszone przez osoby kąpiące się oraz zanieczyszczenia organiczne w postaci moczu i kału. Jakość wody w basenach powinna być porównywalna do jakości wody pitnej. Jakość mikrobiologiczna opiera się na tradycyjnych...
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Antimicrobial properties of chitosan solutions, chitosan films and gelatin-chitosan films
PublicationThe antimicrobial properties of chitosan solutions and films against selected bacteria and the effect of chitosan incorporation into gelatin films were studied. The bactericidal effect of chitosan solutions increased with time and temperature of sample incubation. Two psychrotrophic strains Pseudomonas fluorescens and Listeria innocua were more sensitive to chitosan than mesophilic strains Escherichia coli and Staphylococcus aureus....
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Konstrukcja i wstępna ocena użyteczności szczepionki anty-Toxoplasma gondii opartej na chimerycznych fimbriach Escherichia coli typu Dr z epitopami antygenów SAG1, GRA1 lub MAG1
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Molecular basis of lipopolysaccharide heterogeneity in Escherichia coli: envelope stress-responsive regulators control the incorporation of glycoforms with a third 3-deoxy-α-D-manno-oct-2-ulosonic acid and rhamnose.
PublicationMass spectrometric analyses of lipopolysaccharide (LPS) from isogenic Escherichia coli strains with nonpolar mutations in the waa locus or overexpression of their cognate genes revealed that waaZ and waaS are the structural genes required for the incorporation of the third 3-deoxy-α-D-manno-oct-2-ulosonic acid (Kdo) linked to Kdo disaccharide and rhamnose, respectively. The incorporation of rhamnose requires prior sequential incorporation...
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Estimation of autotrophic maximum specific growth rate constant-experience from the long-term operation of a laboratory-scale Sequencing Batch Reactor System
PublicationW pracy wykorzystano wyniki badań z długookresowej eksploatacji laboratoryjnego reaktora typu SBR do estymacji stałej szybkości przyrostu bakterii nitryfikacyjnych. Badania obejmowały zarówno pomiary empiryczne (doświadczenia przy niskim stosunku substratu do biomasy) oraz symulacje komputerowe. Do symulacji dynamicznych wykorzystano jedną wartość współczynnika = 1,2 1/d, pomimo różnych wartości mierzonych jednostkowych szybkości...
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Novel single-stranded DNA-binding protein from psyvhrophilic bacterium Psychrobacter arcticus
PublicationTo study the biochemical properties of SSB from Psychrobacter arcticus (ParSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 642 nucleotides encoding SSB protein of 213 amino acids with a calculated molecular mass of 22.8 kDa. The amino-acid sequence of ParSSB exhibits 49% identity and 57% similarity to Escherichia coli SSB....
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Suppressors of lapC mutation identify new regulators of LpxC, which mediates the first committed step in lipopolysaccharide biosynthesis
PublicationGram-negative bacteria, such as Escherichia coli, are characterized by an asymmetric outer membrane (OM) with lipopolysaccharide (LPS) located in the outer leaflet and phospholipids facing the inner leaflet. E. coli recruits LPS assembly proteins LapB, LapC and LapD in concert with FtsH protease to ensure a balanced biosynthesis of LPS and phospholipids. We recently reported that bacteria either lacking the periplasmic domain of...
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Thermal and spectroscopic profiles variation of cold-pressed raspberry seed oil studied by DSC, UV/VIS, and FTIR techniques
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Co lekarze estetologii i kosmetolodzy powinni wiedzieć o roli racjonalnego żywienia w profilaktyce chorób nowotworowych
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Euro III / Euro IV Emissions - A Study of Cold Start and Warm Up Phases with a SI (Spark Ignition) Engine
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Application of Silver Nanostructures Synthesized by Cold Atmospheric Pressure Plasma for Inactivation of Bacterial Phytopathogens from the Genera Dickeya and Pectobacterium
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Application of cold atmospheric pressure plasmas for high-throughput production of safe-to-consume beetroot juice with improved nutritional quality
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Charakterystyka faz rozdmuchu preform z poli(tereftalanu etylenu) w procesie rozdmuchiwania z jednoczesnym ich rozciąganiem
PublicationScharakteryzowano proces wytwarzania pojemników PET w procesie rozdmuchiwania z jednoczesnym rozciąganiem półfabrykatów w postaci preform. Największy nacisk położono na szczegółowe omówienie poszczególnych etapów procesu rozdmuchiwania oraz etapów pracy maszyny rozdmuchowej. Omówiono też wybrane zagadnienia przepływu powietrza podczas procesu rozdmuchiwania butelek. Poruszono również problem przemian fazowych tworzywa PET występujących...
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Cold plasma treatment of tannic acid as a green technology for the fabrication of advanced cross-linkers for bioactive collagen/gelatin hydrogels
PublicationTannic acid (TA) is a natural compound studied as the cross-linker for biopolymers due to its ability to form hydrogen bonds. There are different methods to improve its reactivity and effectiveness to be used as a modifier for biopolymeric materials. This work employed plasma to modify tannic acid TA, which was then used as a cross-linker for fabricating collagen/gelatin scaffolds. Plasma treatment did not cause any significant...