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Search results for: REALTIME PCR
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A simple modification to improve the accuracy of methylation-sensitive restriction enzyme quantitative polymerase chain reaction
PublicationDNA digestion with endonucleases sensitive to CpG methylation such as HpaII followed by polymerase chain reaction (PCR) quantitation is commonly used in molecular studies as a simple and inexpensive solution for assessment of region-specific DNA methylation. We observed that the results of such analyses were highly overestimated if mock-digested samples were applied as the reference.We determined DNA methylation levels in several...
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Preconditioners with Low Memory Requirements for Higher-Order Finite-Element Method Applied to Solving Maxwell’s Equations on Multicore CPUs and GPUs
PublicationThis paper discusses two fast implementations of the conjugate gradient iterative method using a hierarchical multilevel preconditioner to solve the complex-valued, sparse systems obtained using the higher order finite-element method applied to the solution of the time-harmonic Maxwell equations. In the first implementation, denoted PCG-V, a classical V-cycle is applied and the system of equations on the lowest level is solved...
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A measurement method for capacitive sensors based on a versatile direct sensor-to-microcontroller interface circuit
PublicationIn the paper, there is presented a new time-domain measurement method for determining the capacitance values of capacitive sensors, dedicated, among others, to capacitive relative humidity sensors. The method is based on a versatile direct sensor-to-microcontroller interface for microcontrollers with internal analog comparators (ACs) and with precision voltage reference sources, e.g. digital-to-analog converters (DACs). The reference...
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Wojciech Chrzanowski dr hab. inż.
PeopleWojciech Chrzanowski was born in 1952 in Toruń. He completed his studies in Chemical Technology in 1975, getting his MSc in Inorganic Technology (specialty: Anti-Corrosion Protection) At the Faculty of Chemistry, Gdańsk University of Technology (with distinction). Later this year he was employed in the Department of Analytical Chemistry of then Institute of Chemical Engineering and Measuring Techniques, initially as a technical...
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Reduktaza NADPH:cytochrom P450 - nie tylko partner cytochromu P450.
PublicationReduktaza NADPH:cytochrom P450 (CPR) zlokalizowana w siateczce śródplazmatycznej większości komórek eukariotycznych jest przedstawicielem rodziny reduktaz diflawinowych. Białko CPR składa się z trzech domen. Jedna z nich, na C-końcu, wiąże cząsteczkę FAD i NADPH, druga, na N-końcu, cząsteczkę FMN, trzecia jest domeną regulacyjną. Enzym ten posiada zdolność jednoczesnego przyjmowania dwóch równoważników jonu hydroniowego z NADPH...
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Thermal Energy Storage with PCMs in Shell-and-Tube Units: A Review
PublicationThe paper presents a survey of the experimental and numerical studies of shell-and-tube systems in which phase change material (PCM) is used. Due to the multitude of design solutions for shell-and-tube systems, the emphasis is placed on double-tube (DT), triplex-tube (TT), and multitube (MT) units. Additionally, only single-pass systems are considered. Particular attention is paid to the method of heat transfer intensification....
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Solar light driven degradation of textile dye contaminants for wastewater treatment – studies of novel polycationic selenide photocatalyst and process optimization by response surface methodology desirability factor
PublicationThe unplanned anthropogenic activities and raced industrial revolution detrimentally causes serious threat to terrestrial and aquatic life. A high discharge of wastewater from industries using dyes affects living organisms and the environment. This paper presents studies on polycationic selenides (PCS) synthesized by hydrothermal methods for photocatalytic degradation of dyes. The synthesized PCS were confirmed by various characterization...
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Power converter interface for urban DC traction substations - solutions and functionality.
PublicationThis paper focuses on extending an urban DC traction substation functionality by means of an additional power converter interface (PCI). In particular, by enabling bidirectional energy exchange between LV DC traction grid, AC grid and V2G chargers. Among other things, the presented material compares general attributes of the most promising DC/DC converters that can be used in a PCI, meet the requirements of galvanic isolation and...
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Metabolomic and antioxidant properties of different varieties and origins of Dragon fruit
PublicationDragon fruit has appealed much concern from consumers as a novelty fruit with potent nutritional and medicinal benefits. Dragon fruit quality warrants comprehensive evaluation, based on the contents of pigments and health- promoting natural compounds in different varieties. This study was aimed to evaluate the differences among dragon fruit varieties extracted with methanol–water (CD3OD-D2O) and methanol (CD3OD) by proton nuclear magnetic...
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Energy Losses Due to Imperfect Payment Infrastructure and Payment Instruments
PublicationOne of the strategic objectives of the European Union is a reduction in greenhouse gas emissions and improvement of energy efficiency by at least 32.5% in different areas of the economy by 2030. However, little is known about the impact of payment in retail on energy consumption. The purpose of this paper is to assess the chain of losses of time and energy, and therefore financial losses, that occur due to the imperfection of payment...
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Rubber wastes recycling for developing advanced polymer composites: A warm handshake with sustainability
PublicationRecycling and management of rubber wastes experiences an early-stage maturity in the quest for sustainable and circular materials. Up to now, solutions proposed for sustainable development of rubber wastes are limited, so that properties and performance features of recycled products are inadequate for practical applications. Herein, an experimental protocol is introduced for manufacturing semi-sustainable polymer composites based...
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Why is the cubic structure preferred in newly formed ice?
PublicationMolecular dynamics was employed to explain the preference for the cubic structure in newly formed crystals of ice. The results showed that in supercooled liquid water the molecules connected by hydrogen bonds are more likely to adopt relative orientations similar to the ones characteristic for cubic ice. The observed preference for certain relative orientations of molecules in the hydrogen-bonded pairs results in the higher probability...
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Jacek Ryl dr hab. inż.
PeopleI have obtained my PhD in chemical technology in 2010. In the same year, I was employed at the Department of Electrochemistry, Corrosion and Materials Engineering at the Faculty of Chemistry. In 2018 I received habilitation at the Faculty of Chemistry GUT in technical sciences in the discipline of chemical technology. From 2021, he was employed at the Institute of Nanotechnology and Materials Science at the Faculty of Technical...
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The analysis of the expression of hTERT in anthraquinone-treated NSCLC cells
Open Research DataThe datasets showcase an analysis of hTERT relative gene expression in NSCLC cells treated with anthraquinone, quantified as fold change compared to DMSO-treated control cells, using the comparative CT method.
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BADANIE EFEKTYWNOŚCI WYKRYWANIA ANOMALII PROCESOWYCH W DZIAŁANIU TURBINY PAROWEJ ELEKTROWNI JĄDROWEJ PRZY POMOCY METOD WIELOWYMIAROWEJ ANALIZY STATYSTYCZNEJ
PublicationW artykule przedstawiono analizę możliwości wykrywania anomalii procesowych w działaniu turbiny parowej elektrowni jądrowej przy pomocy metod wielowymiarowej analizy statystycznej. Zasymulowano symptomy dwóch rodzajów uszkodzeń turbiny parowej tj. uderzenie wodne oraz, wyciek pary z zaworu części niskoprężnej. Jako narzędzie diagnostyczne wykorzystano Metodę Składników Podstawowych PCA (z ang. Principal Component Analysis). Jako...
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Physicochemical aspects of the application of surfactants and biosurfactants in soil remediation
PublicationZaprezentowano właściwości zwilżające, emulgujące oraz solubilizujące surfaktantów niejonowych Rokanolu L10, Tritonu X-100 oraz biosurfaktantu JBR 425. Jako modelowe zanieczyszczenia wybrano tetrachloroetylen (PCE) oraz o-dichlorobenzen (o-DCB). Stabilność emulsji rosła wraz ze stężeniem surfaktantu. Rozpuszczalnośc PCE i o-DCB była największa w roztworach micelarnych biosurfaktantu JBR.
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Identyfikacja sekwencji markerowych w mikologicznej diagnostyce molekularnej
PublicationProcedury diagnostyki mikologicznej coraz częściej wykorzystują metody molekularne, oparte na analizie kwasów nukleinowych. Pozwala to na skrócenie czasu analizy oraz polepszenie wielu innych, istotnych dla procesu diagnostycznego parametrów takich jak: czułość, powtarzalność, specyficzność oraz potencjał różnicujący. Istotnym etapem przy projektowaniu układów do diagnostyki molekularnej grzybów jest wybór celu molekularnego. Powszechnie...
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Construction and purification of his6-Thermus thermophilus MutS protein.
PublicationGen mutS z termofilnej bakterii Thermus thermophilus zamplifikowano przy użyciu PCR, sklonowano i przeprowadzono jego ekspresję w E. coli. Rekombinantowe białko MutS zawierające oligohistydynową domenę na N-końcu oczyszczono w jednoetapowej procedurze przy wykorzystaniu chromatografii powinowactwaNi(2+). Zdolność do rozpoznania niekomplementarności białka his(6)-MutS potwierdzono w eksperymentach ochrony DNA przed trawieniem...
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Analysis of the reliability of any-hop star network
PublicationZałożono, że sieć ma wielostopniową strukturę gwiaździstą. Jako oceny niezawodności tych sieci przyjęto: 1) średnią liczbę sprawnych par węzłów, które mogą komunikować się wzajemnie; 2) średnią liczbę sprawnych par węzłów, które mogą komunikować się poprzez węzeł centralny; 3) średnią liczbę sprawnych par węzłów, które mogą komunikować się z węzłem centralnym; 4) prawdopodobieństwo zdarzenia, że sprawne węzły mogą komunikować...
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Assessment of OpenMP Master–Slave Implementations for Selected Irregular Parallel Applications
PublicationThe paper investigates various implementations of a master–slave paradigm using the popular OpenMP API and relative performance of the former using modern multi-core workstation CPUs. It is assumed that a master partitions available input into a batch of predefined number of data chunks which are then processed in parallel by a set of slaves and the procedure is repeated until all input data has been processed. The paper experimentally...
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A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants
PublicationThe World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost surveillance and...
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Telomerase inhibition - unfulfilled hopes in the perfect molecular target
PublicationTelomerase plays a pivotal role in cell proliferation, homeostasis, and neoplastic transformation, making it a promising molecular target for cancer chemotherapy. Of note, although hTERT has been explored thoroughly as a target, none of the promising molecules has been approved as a drug until now. The subject of research conducted as part of my doctoral dissertation is explaining the cellular and molecular mechanism of action...
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STUDY ON THE RELATIONSHIP BETWEEN VEHICLE MAINTENANCE AND FUEL CONSUMPTION
PublicationA contemporary road vehicle (RV) is a rather complex system, consisting of a large number of subsystems, assemblies, units, and elements (parts). While operating, an RV interacts with the environment, and its elements interact with each other. Consequently, the properties (parameters) of these elements change in the process - hardness, roughness, size, relative position, gapping, etc. A partial solution to the presented problems...
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Evaluation and rationale of the performance of several elastomeric composites incorporating devulcanized EPDM
Publicationthylene Propylene Diene rubber (EPDM), is present in many applications. However, its crosslinked structure makes difficult its recycling. The use of devulcanized EPDM, (dEPDM) as a component of elastomeric composites, is a real possibility to reuse EPDM scraps. In this study, three matrices (natural rubber (NR), styrene butadiene rubber (SBR) and EPDM rubber) have been tested including devulcanized EPDM as a recycled component....
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Offshore surface waters of Antarctica are free of microplastics, as revealed by a circum-Antarctic study
PublicationIn 2018, during a circum navigation of Antarctica below 62° S by the sailing boat Katharsis II, the presence of plastics was investigated with surface sampling nets at ten evenly spaced locations (every 36° of longitude). Although fibres that appeared to be plastic (particles up to 2 cm) were found in numbers ranging from 1 particle (0.002 particles per m3) to 171 particles (1.366 particles per m3) per station, a Fourier-transform...
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Materiały zmiennofazowe do stabilizowania temperatury nawierzchni asfaltowych
PublicationW artykule przedstawiono możliwość wykorzystania lekkiego kruszywa budowlanego modyfikowanego materiałem zmiennofazowym (PCM), jako dodatku do nawierzchni asfaltowej, w celu zwiększenia jej stabilności termicznej przy zachowaniu dotychczasowych właściwości mechanicznych. Cel ten może zostać osiągnięty przez nasączenie porowatych granulek lekkiego kruszywa odpowiednim materiałem zmiennofazowym, co umożliwi magazynowanie określonych...
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Novel method of lung area extraction in chest perfusion computed tomography
PublicationChest perfusion computed tomography (pCT) is a useful technique in the medical diagnosis of how organs function. Perfusion CT scans are used to calculate perfusion parameters. In the case of automated methods of lung perfusion parameters calculation, the prior extraction of the lung area is desired to avoid unnecessary calculation in an area outside the lung cross-section and to avoid wasting time on processing signals of no diagnostic...
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Surface irregularities as a complex signal of tool representation together with uneven displacement in respect to the workpiece
PublicationIn a dynamic machining process, distortion in surface irregularity is a very complex phenomenon. Surface irregularities form a periodic representation of the tool profile with various kinds of disturbance in a broad range of changes in the height and length of the profile. To discern these irregularity disturbances, interactions of the tool in the form of changes perpendicular and parallel relative to the workpiece were analyzed...
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Dissociation pathways of protonated water clusters
PublicationRelative abundances of positively ionized water clusters formed in a low-pressure electrical discharge as function of reduced electric field were obtained by using drift cell and quadrupole mass spectrometer. It was noticed that some clusters say with n=4 were more abundant than others, at comparable drift conditions. Dissociation energies have been derived from equilibrium conditions for relative clusters abundances. The Hartree-Fock...
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Information System Purchase and Integration Contingencies When Companies Merge
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Attachment of silica and alumina nanoparticles as studied by QCM
PublicationNanocząstki są wykorzystywane w mikroelektronice do produkcji bardzo płaskich i gładkich powierzchni. Proces produkcji taki materiałów nazywany jest planaryzacją chemiczno-mechaniczną (CMP). Poniewąż nanocząstki na skutek interakcji z produkowanym materiałem specjalistyczntycznym mogą wiązać do jego powierzchni, na skutek czego powodując zanieczyszczenia. Ze względu na wpływ zanieczyszczeń na wydajność i miarodajność produkowanego...
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The overview of reactors used for the production of precipitated calcium carbonate via carbonation route
PublicationCarbonation is one of the methods for the production of precipitated calcium carbonate (PCC) on the industrial scale. A gaseous CO2 is used as a reagent in this process. The hydrodynamic conditions gener-ated in the reactor affect the course of the process and the characteristics of the precipitated calcium carbonate particles. This paper discusses the construction of standard reactors and new proposed con-struction used for the...
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Monitoring of organic pollutants in coastal waters of the Gulf of Gdańsk, Southern Baltic.
PublicationPrzedstawiono zmiany zawartości zanieczyszczeń organicznych (np. VOC, PAH, PCB, chlorofenoli) w wodach Zatoki Gdańskiej na przestrzeni 5-ciu lat. Zawartość VOX i VOC zmieniała się od kilku ngdm-3 do kilkuset ngdm-3, podczas gdy zawartość chlorofenoli fenoksykwasów była na poziomie kilku ngdm-3. W większości próbek nie wykryto PCB.
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Market Size, Competitiveness and Technological Frontier - the Impact of Trade Integration with the UE on Productivity in Polish Manufacturing Sectors
PublicationThis paper addresses the relationship between growth of relative productivity in Polish manufacturing sectors and forces stemming from trade integration with the EU. We look at the productivity growth from the perspective of relations between Polish manufacturing sectors and the foreign ones, focusing on partner countries from the enlarged EU. Empirical analysis is based on sector level bilateral data concerning both domestic (Polish)...
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Disturbances of magnetic field in surrounding of mobile, metalic plate
PublicationThe uniform magnetic field of the Earth in the surrounding of a mobile, metalic object undergoes deformation by eddy currents. The influence of the moving conducting object on total magnetic field of the Earth depends on the object's shape, position and speed of the changes relative to the magnetic field's vector of the Earth as well as the electric conductivity and the relative magnetic permeability of the material. The disturbances...
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Photogeneration of charge carriers pairs in pentacene layers.
PublicationAnalizowano mechanizmy odpowiedzialne za powstawanie par nośników ładunku w polikrystalicznych warstwach pentacenu.
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Konsultacje (MMM, Robotyka) ACR sem. 4
e-Learning CoursesMateriały do zajęć: Metody Modelowania Matematycznego, Podstawy Robotyki
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OiS-PCh&MG-ćwiczenia2022 (90)
e-Learning CoursesWebinaria dla grup ćwiczeniowych ACiR1 i ACiR2 z przedmiotu Obwody i sygnały dla kierunku ACiR sem. 2, studia I stopnia. Prowadzący: Piotr Chudziak (piochudz@pg.edu.pl) Marek Grzegorek (marek.grzegorek@pg.edu.pl) Czesław Stefański (cestef@pg.edu.pl) (wersja 2022, dodatek do "OiS'22-wsparcie ćwiczeń")
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Zasady przedsiębiorczości i zarządzania ACR 2023
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Post-Effort Changes in Autophagy- and Inflammation-Related Gene Expression in White Blood Cells of Healthy Young Men
PublicationAcute, strenuous physical exertion requiring high levels of energy production induces the production of reactive oxygen species and metabolic disturbances that can damage the mitochondria. Thus, selective autophagic elimination of defective mitochondria may improve resistance to oxidative stress and potentially to inflammation. The main goal of this study was to evaluate the impacts of intense effort on changes in the expression...
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Charakterystyki pojemnościowo-zasięgowo sieci komórkowej LTE wykorzystującej metodę Partial Frequency Reuse
PublicationW referacie przedstawiono wyniki badań charakterystyk pojemnościow-zasięgowych sieci komórkowej LTE, w której zastosowano metodę ponownego wykorzystania pasma PFR. Wyniki badań umożliwiają ocenę efektywności wykorzystania zasobów fizycznych, w zależności od metody podziału obszaru komórki na podobszary o różnym sposobie przydziału tych zasobów. Mogą być one użyteczne do oceny efektywności metody PFR oraz do projektowania i optymalizacji...
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Development of nanoscale morphology and role of viscoelastic phase separation on the properties of epoxy/recycled polyurethane blends
PublicationA novel and cost-effective approach towards the modification of epoxy matrix has been developed using recycled polyurethane for the first time without sacrificing any of the intrinsic properties of the resin. Polyurethane, recycled from waste foam by glycolysis process (RPU), was found to be very effective in improving the properties of the thermosetting resin based on Diglycidyl ether of bisphenol-A (DGEBA). The effect of the...
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Catalytic activity of Ni-MgAl2O4 modified with transition metal (Ti, Mo, W) carbides as potential catalysts for resource recovery via dry reforming of waste plastics
PublicationThe growing amounts of plastic waste and CO2 emissions are two environmental threats that require urgent attention. Pyrolysis combined with dry reforming (PCDR) is a technology that allows both CO2 utilization and resource recovery from waste plastic. New catalysts for PCDR are crucial for developing efficient and stable processes that can be widely implemented in the industry. In this study, Ni/MgAl2O4 catalysts modified using different...
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Identification of evolutionary conserved DNA sequence and corresponding S21 ribosomal protein region for diagnostic purposes of all Borrelia spirochetes
PublicationIt is still under investigation, whether all Borrelia sp. causing Lyme borreliosis and other diseases are already identified and properly classified as human pathogens. For this reason, it is of great importance to develop a diagnostic ELISA test that detects all Borrelia sp. The aim of this study was to identify conserved DNA and protein regions present in all currently known Borrelia sp. In experimental studies 31 available Borrelia...
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Structure and the Biochemical Potential of the Bacterial Community in Wastewater Treatment Plant Effluent and in Receiving Waters: Northern Poland Case Study
PublicationWastewater treatment processes are monitored mainly in terms of biogenic substance removal efficiency. Little is known about the treated wastewater microbiome and its influence on the recipient. In this study, a wide range of methods (cultivation, microscopic analysis, New Generation Sequencing and Real Time PCR) were employed to determine the bacterial community composition of the treated wastewater and its receiver. Preliminary...
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Dissociation energies of protonated water clusters
PublicationRelative abundances of positively ionized water clusters formed in a low-pressure electrical discharge as function of reduced electric field were obtained by using drift cell and quadrupole mass spectrometer. It was noticed that some clusters say with n = 4 were more abundant than others, at comparable drift conditions. Dissociation energies have been derived from equilibrium conditions for relative clusters abundances. The Hartree-Fock...
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Synthesis and characterization of novel aliphatic polyurethanes for tissue engineering applications
PublicationSummarizing, in this thesis was described the synthesis of novel PUR system, which was obtained by using aliphatic diisocyanate (HDI), amorphous macrodiol (PEBA) and chain extender (BDO). This PUR system was established as suitable for TE purpose and successfully modified with AA, which as expected improved its biocompatibility. According to this, AA-modified HDI-based PURs is the PUR system ready for further studies including...
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Preliminary Testing of the Influence of Modified Polycaprolactones on Anabaena Variabilis Growth in Seawater
PublicationSeveral new biodegradable polymer materials have recently come onto the global market. Mostly the results on degradation kinetic studies are presented. This paper suggests using one of the tests to estimate the impact of polymer packaging material on sea life. The microorganism chosen was Anabaena variabilis (identified in many waters, including those of the Baltic Sea, especially in the Gulf of Gdansk and Puck Bay; this cyanobacterium...
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OPTIMAL FARM SIZE AND EFFECTIVENESS OF AGRICULTURE IN THE EU: THE CASE OF WHEAT YIELDS
PublicationThe article concentrates on the relationship between the size of a farm and its efficiency in relation to wheat production in the European Union (EU). The issue is not new, however fundamental from the point of view of the agricultural policy, as the appropriate shape of the agrarian structure affects the economic rationality in agriculture and significantly improves the productivity. We analysed data for the period between 2004...
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D-loop sequences retrieved from Canis lupus familiaris mitochondrial genome
Open Research DataCanine mitochondrial genome is built of 16727 bp. Non-coding control region (mtCR), called also D-loop, begins with 15458 nucleotide and ends with 16727 nucleotide. The length of this fragment is 1270 bp (Kim et al., 1998). D-loop region is responsible for replication and transcription of mitochondrial DNA. Mutations that occur within it may cause irregularity...