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Department of Chemistry, Technology and Biochemistry of Food
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Catalog Publications
Year 2017
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Zasady zapewniania bezpieczeństwa żywności
PublicationŻywność jest niezbędnym elementem życia człowieka, a jej spożywanie jest czynnością codzienną i bardzo ważną dla funkcjonowania organizmu. Produkcja i spożycie żywności wywołuje konsekwencje ekonomiczne, społeczne i środowiskowe. Pominięcie aspektów związanych z bezpieczeństwem żywności skutkowałoby występowaniem chorób układu pokarmowego oraz strat ekonomicznych o zasięgu zarówno regionalnym, jak i światowym. Bezpieczeństwo żywności...
Year 2016
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A fast and convenient method for analysis of collagen hydrolysis products Szybka i dogodna metoda analizy produktów hydrolizy kolagenu
PublicationPolypeptides produced by hydrolysis of collagen from minced animal hide and skin scraps were studied by mass spectrometry (MS) in conjunction with electrophoresis to det. their mol. mass. The degree of peptides ionisation was ca 50+. This allowed for observation of the large polypeptide chains with mass analyzers with a relatively low m/z range. The electrospray and atm. pressure photoionization techniques were used. The polypeptide...
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A novel method for drop in drop edible oils encapsulation with chitosan using a coaxial technique
PublicationThe presented work concerns a novel one step method for oil encapsulation. In this coaxial system the oil constitutes the core of the capsule, while the chitosan solution is the polymer shell surrounding the core to provide separation of the core from the external environment. The paper contains a diagram of the encapsulation equipment and explains the principle of its operation. The experimental results showed the impact of the...
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Antyoksydacyjne i przeciwdrobnoustrojowe właściwości bioaktywnych fitozwiązków żurawiny
PublicationW racjonalnym żywieniu człowieka podkreśla się rolę owoców i warzyw, będących źródłem tzw. bioaktywnych fitozwiązków. Wśród owoców szczególną uwagę zwraca żurawina ze względu na wiele udokumentowanych właściwości prozdrowotnych. Owoc ten wyróżnia duża zawartość związków fenolowych o charakterze przeciwutleniaczy, które wspierają naturalny antyoksydacyjny system obronny organizmu przed uszkodzeniami wywołanymi stresem oksydacyjnym...
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Comparison of oil yield and quality obtained by different extraction procedures from salmon (Salmo salar) processing byproducts
PublicationThe content and composition of lipids in different byproducts (skins, heads, and backbones) from mechanically processed farmed Atlantic salmon were determined and compared with that obtained from wild salmon. Three different procedures were used to establish the optimal conditions of oil extraction (at high temperature −95°C, “cold” extraction at temperature not exceeding 15°C and enzyme assisted with Alcalase). “Cold” extraction...
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COMPOSITION OF BIOACTIVE SECONDARY METABOLITES AND MUTAGENICITY OF SAMBUCUS NIGRA L. FRUIT AT DIFFERENT STAGES OF RIPENESS
PublicationThe relationship between the content of bioactive compounds and mutagenic activity of elderberry fruit at different stages of ripeness was investigated. Signifi cant differences in the antioxidant profi les (TLC, HPLC with post-column derivatization) and antioxidant activity (ABTS, DPPH, and FC tests) were observed for studied elderberry extracts. The more ripen the fruit at the time of harvest were, the higher the content of...
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Effect of conventional cooking on changes in the contents of basic composition and glucosinolates in kale
PublicationBrassica vegetables have been strongly recommended as part of human diet because of its high content of bioactive sulphur compounds, eg glucosinolates. The nutrient and health promoting compounds in kale are significantly affected by traditional cooking. The study investigated changes in the levels of dry mass, ash, fat, total protein, dietary fibre as well as total and individual glucosinolates in the kale due to the traditional...
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Effect of cooking on the contents of glucosinolates and their degradation products in selected Brassica vegetables
PublicationChanges in the levels of glucosinolates and their degradation products in selected Brassica vegetables due to the cooking process were investigated. The purple cauliflower was found to be the best source of aliphatic and indole glucosinolates, and it was also abundant in compounds such as sinigrin, glucoraphanin and glucobrassicin. As a result of cooking rutabaga, green cauliflower and purple cauliflower, a significant decrease...
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Effect of culinary treatment on chnages in the contents of selected nutrients and non-nutrients in curly kale (Brassica oleracea var. acephala)
PublicationKale has a great nutritive value, very high antioxidant activity and pro-healthypotential. The level of phytochemicals and related bioactive compounds arestrongly dependent on pre and postharvest stages of production chain (domestic orindustrial). The study investigated changes in the levels of vitamin C, b-carotene,total polyphenols, antioxidant activity, degradation products of glucosinolates aswell as nitrates and nitrites in...
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Fosfolipidy oraz produkty ich hydrolizy jako żywieniowe czynniki prewencyjne w chorobach cywilizacyjnych
PublicationWyniki licznych badań epidemiologicznych wskazują, że fosfolipidy odgrywają istotną rolę w profilaktyce chorób przewlekłych, z którymi borykają się współczesne społeczeństwa. Omawiane związki są odpowiedzialne za prawidłowe funkcjonowanie błon komórkowych przez zapewnienie odpowiedniej ich płynności oraz przepuszczalności i właściwej aktywności białek błonowych, w tym receptorów. Wspomniane mechanizmy pełnią...
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Isolation and characterization of Acid Soluble Collagen from the Skin of African Catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic cod (Gadus morhua)
PublicationAcid-soluble collagen (ASC) from the fish skin of African catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic cod (Gadus morhua) was extracted and characterized. The ASC extraction yield was 75%, 73% and 68%, respectively. The denaturation and melting temperatures of African catfish ASC (29.3°C and 100.0°C) were significantly higher than ASC of Salmon and Baltic cod (20.6°C and 90.5°C; 15.2°C and 86.7°C, respectively), assessed...
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Kwasy nukleinowe w żywności występowanie i właściwości reologiczne
PublicationW naukach o żywieniu kwasy nukleinowe uznawane były dotychczas za mało istotny składnik żywności. Ich właściwości odżywcze nie są uwzględniane przy formułowaniu zaleceń żywieniowych, mimo że należą do podstawowych składników żywności, zwłaszcza surowej lub nisko przetworzonej. Są one obecne głównie w szybko rosnących komórkach tkanek oraz tych, które zachowały zdolność do wzrostu i regeneracji. W niniejszej pracy przedstawiono...
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Nondestructive Optical Sensing of Flavonols and Chlorophyll in White Head Cabbage ( Brassica oleracea L. var. capitata subvar. alba ) Grown under Different Nitrogen Regimens
PublicationA multiparametric optical sensor was used to nondestructively estimate phytochemical compounds in white cabbage leaves directly in the field. An experimental site of 1980 white cabbages (Brassica oleracea L. var. capitata subvar. alba), under different nitrogen (N) treatments, was mapped by measuring leaf transmittance and chlorophyll fluorescence screening in one leaf/cabbage head. The provided indices of flavonols (FLAV) and...
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Preparation and characterization of porous scaffolds from chitosan-collagen-gelatin composite
PublicationNovel porous scaffolds composed of chitosan, collagen and gelatin were prepared and characterized. For preparing scaffolds gelatin and collagen isolated from fish skins with various physicochemical properties were used. In order to reduce preparation solubility glutaraldehyde in the amount of 1%, w/w relative to the total biopolymers weight in solution was used. All obtained biomaterials showed a homogeneous porosity. Protein polymer...
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Solid phase microextraction–comprehensive twodimensional gas chromatography–time-of-flight mass spectrometry: a new tool for determining PAHs in airport runoff water samples
Publicationfundamental aspect of airport operations is the pollution caused by airport runoff waters. Polycyclic aromatic hydrocarbons (PAHs) are one of the most important groups of xenobiotics which are commonly found in runoff water originating from airports. Only very limited data on the analysis of airport runoff water have been published until now. Therefore, a reliable and accurate analytical method based on headspace solid-phase microextraction...
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The effect of high pressure on formation of volatile amines in minced meat of cod (Gadus morhua)
PublicationThe effects of high pressure at subzero temperature (193 MPa, at −20 °C) on inactivation of natural microflora of cod meat and degradation of trimethylamine oxide (TMAO) to trimethylamine (TMA) during refrigerated storage and to dimethylamine (DMA) and formaldehyde (FA) during frozen storage were investigated. The content of TMA, DMA and FA in cod meat did not change immediately after pressure treatment. During 40 days of frozen...
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Wpływ zmiennych procesu kapsułkowania współosiowego na stabilność oksydacyjną oleju rzepakowego
PublicationPraca stanowi kontynuację badań nad stabilnością olejów jadalnych kapsułkowanych metodą współosiową, w strukturze naturalnych polimerów. W badaniach określono wpływ wybranych czynników procesu kapsułkowania na stabilność modelowego oleju rzepakowego na podstawie oznaczeń zawartości pierwotnych i wtórnych produktów utlenienia. Uzyskane wyniki dowodzą, że najmniejsze zmiany oksydacyjne zachodzą podczas kapsułkowania oleju za pomocą...
Year 2015
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Antimicrobial properties of chitosan solutions, chitosan films and gelatin-chitosan films
PublicationThe antimicrobial properties of chitosan solutions and films against selected bacteria and the effect of chitosan incorporation into gelatin films were studied. The bactericidal effect of chitosan solutions increased with time and temperature of sample incubation. Two psychrotrophic strains Pseudomonas fluorescens and Listeria innocua were more sensitive to chitosan than mesophilic strains Escherichia coli and Staphylococcus aureus....
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Blue-berried honeysuckle a promising cancer preventing fruit; Polish genotypes as a source of bioactive phytochemicals
PublicationFrom the ancient times, plants containing health-oriented compounds in their matrices are used in the treatment of different complaints and diseases. One of these groups are plant secondary metabolites (PSM), especially phenolic compounds, terpenes and terpenoids. A large number of PSM exhibit cytotoxicity against a variety of tumor cells, as well as cancer preventive and anticancer efficacy in preclinical animal models. For this...
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Budowa i podstawowy skład surowców oraz produktów żywnościowych
PublicationRozdział zawiera zagadnienia związane ze składem chemicznym mięsa, mleka, surowców skrobiowych, nasion roślin strączkowych, tłuszczów jadalnych oraz owoców i warzyw.
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Cabbage Juices and Indoles Modulate the Expression Profile of AhR, ERα, and Nrf2 in Human Breast Cell Lines
PublicationOur previous studies showed the diversified effect of cabbage juices and indoles on the estrogen metabolism key enzymes (CYP1A1, CYP1A2, CYP1B1) in breast epithelial cells differing in ER status, i.e., in tumorigenic—MCF7, MDA-MB-231 and non-tumorigenic—MCF10A cells. The aim of the present study was to further investigate the mechanism of chemopreventive action of cabbage juice and its active components by evaluating their effect...
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Human breast milk concentration of neopterin at various stages of lactation and during a single feeding
PublicationThe aim of this work was to determine the physiological level of neopterin in human breast milk, and to study its variability depending on the duration of a single feeding and the lactation stage. Breast milk samples from 74 women were collected between 2 and 4 days after delivery, and at 15, 30, and 90 days after delivery. Additionally, breast milk samples from eight women were collected before and after 7 and 15 min of breastfeeding....
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Inspired by Nature: The Use of Plant-derived Substrate/Enzyme Combinations to Generate Antimicrobial Activity in situ
PublicationThe last decade has witnessed a renewed interest in antimicrobial agents. Plants have received particular attention and frequently rely on the spontaneous enzymatic conversion of an inactive precursor to an active agent. Such two-component substrate/enzyme defence systems can be reconstituted ex vivo. Here, the alliin/alliinase system from garlic seems to be rather effective against Saccharomyces cerevisiae, whilst the glucosinolate/myrosinase...
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Juices from non-typical edible fruits as health-promoting acidity regulators for food industry
PublicationThe study verifies the possibility of application of juices from selected fruits characterized by the high antioxidant potential as natural acidity regulators with improved nutritional properties. The tested non-typical fruits included mirabelle plum, sea buckthorn and blue-berried honeysuckle. Beetroot juice whose pH is about 6.0 served as a model food product. Potentiometric titration was used to compare the efficacy of tested...
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Mutagenne i rakotwórcze składniki żywności
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Nauki o żywieniu w erze postgenomicznej
Publicationbrak
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Niebiałkowe związki azotowe
PublicationNiebiałkowe związki azotowe (NPN - non protein nitrogen) są grupą składników wchodzących w skład produktów żywnościowych ważną nie tylko z punktu widzenia producentów żywności, ale także dla specjalistów do spraw żywienia. Do grupy tej należą przede wszystkim wolne aminokwasy i krótkie peptydy, aminy, kwasy nukleinowe i nukleotydy oraz inne mało cząsteczkowe składniki zawierające azot (glikozydy cyjanogenne, alkaloidy, tiazole,...
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Nieodżywcze substancje prozdrowotne pochodzenia roślinnego
Publicationbrak
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Phospholipids as potential prevention factor in carcinogenesis
PublicationPhospholipids are a group of complex lipids, which can be used in cancer chemoprevention. Glicerophospholipids are composed of fatty acids esterified to a glycerol backbone, a phosphate group and a hydrophilic residue such as: cho- line, ethanoloamine, serine or inositol. In the sn-2 position of glicerophospholipid usually there are unsaturated fatty acids, i.e. linolenic acid, while in the sn-1 position more typi- cal are saturated...
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Phytoecdysteroid containing plants – a source of bioactive compounds with potential anticarcinogenic effects
PublicationEcdysteroids are a group of steroids synthesized in inver- tebrates as molting hormones and also in some plants as secondary metabolites acting as a defense against parasites. These compounds do not naturally occur in mammals and are not structurally related to mammalian steroid hor- mones, consequently do not activate mammalian hormone receptors. Ecdysteroids exhibit very low toxicity to mam- mals and to date no serious side effects...
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Relationship between the betalain composition in Opuntia ficus indica and Beta vulgaris varieties and biological activity of their extracts
PublicationBetalains are a class of pigments that are much less com- monly found in nature than anthocyanins or carotenoids. They occur only in a few edible plants such as red beetroot (Beta vulgaris), prickly pears (Opuntia ficus indica), amaranth (Amaranthus sp.) and some mushrooms. This group of pig- ments can be divided into two major groups: yellow vulgax- anthins and red betanins. Despite their structural similarity to compounds from...
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Rola rodników, utleniaczy i przeciwutleniaczy w żywności
PublicationLiczne procesy, chemiczne, enzymatyczne i mikrobiologiczne zachodzące podczas przechowywania i przetwarzania, mają wpływ na kształtowanie jakości żywności. Istotne znaczenie ma utlenianie przy udziale wolnych rodników i reaktywnych form tlenu (RFT). Zmiany powodowane utlenianiem m.in. pogarszają sensoryczne właściwości i wartość odżywczą żywności oraz mogą wywoływać zagrożenia zdrowotne wynikające ze szkodliwości niektórych produktów...
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Sacharydy - występowanie i znaczenie
PublicationRozdział porusza takie zaganienia jak: struktura i nazewnictwo sacharydów, sposoby ich oznaczenia w żywności, zawartość w żywności, chemiczne i fizyczne właściwości oraz przemiany w warunkach przechowywania i przetwarzania, celowe modyfikowanie skrobi, a także rola sacharydów w tworzeniu sensorycznych cech żywności i żywieniu człowieka.
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Simultaneous Determination of Indolic Compounds in Plant Extracts by Solid-Phase Extraction and High-Performance Liquid Chromatography with UV and Fluorescence Detection
PublicationA high-performance liquid chromatographic method with UV and fluorescence detection (HPLC-DAD-FLD) was developed for simultaneous determination of indolic compounds in plant material. Indole-3-carbinol (I3C), indole-3-acetic acid (I3AA), indole-3-acetonitrile (I3ACN), and 3,3′-diindolylmethane (DIM) were used as representative compounds that cover a wide spectrum of indole structures occurring in nature. For concentration and purification...
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Składniki wpływające na reologiczne cechy żywności.
PublicationRozdział opisuje podstawowe zagadnienia reologii w przemyśle spożywczym.
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The extended version of restriction analysis approach for the examination of the ability of low-molecular-weight compounds to modify DNA in a cell-free system
PublicationOne of the primary requirements in toxicology is the assessment of ability of chemicals to induce DNA covalent modification. There are several well-established methods used for this purpose such as 32P-Postlabeling or HPLC-MS. However, all of these approaches have difficult to overcome limitations, which prevents their use in genotoxin screening. Here, we describe the simple protocol exploiting specificity of restriction enzymes...
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The influence of roasting and additional processing on the content of bioactive components in special purpose coffees
PublicationCoffee being the beverage consumed worldwide is also a very competitive commodity. Consequently, producers seek ways of attracting consumers by proposing e.g. novel ingredient combinations usually without evaluating their health quality. In this study, variations in health-promoting determinants for five special purpose coffee brews were characterized. The major bioactive components - chlorogenic acids (CAs) - detected by HPLC-DAD-MS...
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The role of the sawage treatment plant in the removal of genotoxic compounds from the environment
PublicationOne reason for increased incidence of various types of cancer are environmental pollutants particularly of anthropogenic origin. All human activities may pollute the environment, but some have significant impact, just to mention energy industry, mining, transportation, chemical and petrochemical industries and housing. Environmental pollutants are a large group of compounds in terms of their physicochemical properties, some of...
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Zastosowania chromatografii wykluczania (GPC/SEC). Część II – Wykorzystanie GPC/SEC w rozdzielaniu tłuszczów i produktów ich konwersji – artykuł przeglądowy
PublicationTłuszcze i produkty ich konwersji stanowią złożoną grupę związków organicznych, o różnorodnej i skomplikowanej strukturze. Istotnym problemem, zwłaszcza w rozdzielaniu szczegółowym tak złożonych i zróżnicowanych analitów na poszczególne indywidua chemiczne jest ogromna różnorodność budowy związków chemicznych przynależnych do poszczególnych klas lipidów. Z drugiej strony, poszczególne klasy lipidów...
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Związki wpływające na smak żywności
PublicationRozdział opisuje klasyfikację i potencjatory smaków, a także wpływ obróbki surowców na właściwości sensoryczne produktów spożywczych.
Year 2014
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Antioxidants: a premature scientific hypothesis that reshuffled the traditional food pyramid
PublicationAntioxidants present in substantial amounts in foods, plant-based products in particular, have been suggested as chemopreventive agents that can curb the development of undesirable health effects caused by oxidative stress simply by enriching diet in such compounds. This idea has been enthusiastically accepted by consumers, as well as food and pharmaceutical industry, and created great demand for products containing antioxidant...
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Chitosan-protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials.
PublicationAIMS: The development of technology for preparing chitosan-protein scaffolds loaded with lysostaphin, which potentially could be used as dressing for wound treatment and soft tissue infections caused by Staphylococcus aureus. METHODS AND RESULTS: The unique technology of chitosan solubilization using gaseous CO(2) instead of organic or inorganic acids was used for the incorporation of lysostaphin, the enzyme that exhibits bactericidal...
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Comparison of the content of phenolic compounds and antioxidant activity of white, red and black currants (Ribes sp.) extracts
PublicationThe aim of this study was to compare the content of antioxidant compounds and the antioxidant activity of extracts from white, red and black currants (Ribes sp.). The chemical properties verified included determinations of anthocyanins and other phenols by HPLC-DAD-MS, total antioxidant activity by standard spectrophotometric tests (ABTS, DPPH and FCR), and profiles of antioxidants by TLC with visualization reagents (ABTS, DPPH,...
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Efficient production of Staphylococcus simulans lysostaphin in a benchtop bioreactor by recombinant Escherichia coli.
PublicationLysostaphin is an enzyme with bactericidal activity against Staphylococcus aureus and other staphylococcal species. In spite of many advantages and promising results of preliminary research, the enzyme is still not widely used in medicine, veterinary or as a food preservative. One of the most important factors limiting application of the enzyme in clinical or technological practice is the high costs of its production. In the present...
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"Food Oxidants and Antioxidants" Book review
PublicationThe book is the 14th title in the CRC series Chemical and Functional Properties of Food Components, edited since 2001 by Zdzisław E. Sikorski. The editor of the book, a professor in the University of Łódź and member of the Polish Academy of Sciences, is an internationally recognized authority in biochemistry and biophysics of reactive oxygen species. In Poland Grzegorz Bartosz has a reputation as the author of his most interesting...
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HPLC-coupled post-column derivatization aims at characterization and monitoring of plant phytocomplexes, not at assessing their biological properties
PublicationTwo recent decades of research identified a number of environmental factors as well as mechanisms leading to pathogenic processes in the human organism. A common factor in pathogenesis of many diseases is oxidative stress, a condition in which the production of damaging reactive oxygen species (ROS), present in the body as an inevitable consequence of respiration and exacerbated by the inflammatory response and by exogenous factors...
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Interactions of fish gelatin and chitosan in uncrosslinked and crosslinked with EDC films: FT-IR study
PublicationFilms based on fish gelatin, chitosan and blend of fish gelatin and chitosan before and after cross-linking with EDC have been characterized by FT-IR spectroscopy. The FT-IR spectrum of fish gelatin film showed the characteristic amide I, amide II and amide III bands, and the FT-IR spectrum of chitosan film confirmed that the polymer was only a partially deacetylated product, and included CH3-C=O and NH2 groups, the latter both...
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Isothiocyanates may chemically detoxify mutagenic amines formed in heat processed meat
PublicationMeat consumption represents a dietary risk factor increasing the incidence of common cancers, probably due to carcinogenic amines (HAAs) formed upon meat heating. Interestingly, cancers whose incidence is increased by meat consumption, are decreased in populations consuming brassica vegetables regularly. This inverse correlation is attributed to brassica anticarcinogenic components, especially isothiocyanates (ITCs) that stimulate...
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Late cultivars of cabbage can better cope with cadmium- involved stress than early one
PublicationThe usefulness of white cabbage (Brassica oleracea ssp. capitata L. f. alba) for phytoremediation of heavy metals (cadmium and zine) due to the rapid growth and a large biomass of its heads has been already described (Bączek-Kwinta et al., 2011. Kusznierewicz et al., 2012). As the photosynthetic apparatus supplies the whole plant with sugars necessary for various physiological processes and, in consequence, results in the yield,...
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Metoda kapsułkowania z wykorzystaniem systemu współosiowego
PublicationW ciągu ostatnich kilkudziesięciu lat mikrokapsułki stały się ważnym narzędziem w dostarczaniu substancji aktywnych przy jednoczesnej ochronie substancji niestabilnych. Gałęzie przemysłu w których mikrokapsułki znalazły zastosowanie to przemysł farmaceutyczny i biomedyczny, produkcja kosmetyków, przemysł spożywczy, ochrona roślin, budownictwo i wiele innych. Dotychczasowe, powszechnie wykorzystywane do tego celu metody, takie jak...