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Wyniki wyszukiwania dla: GAS CHROMATOGRAPHY COUPLED WITH MASS SPECTROMETRY (GC-MS)
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Determination of 3,4-methylenedioxy-α-pyrrolidinobutiophenone (MDPB) in blood. A case report
PublikacjaSynthetic drugs, known as ‘‘legal highs’’, are still very popular on black market. The determination of their composition are required in courts of law and also to understand what potential users may be subjected to. The interest of new cathinones, e.g. MDPBP, is in view of psychoactive effects that they cause and that, in many cases, they are not controlled by law. The case outlined here concerns 19 years-old man, who was found...
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Derywatyzacja in situ w połączeniu z techniką DLLME-oznaczanie amin biogennych w winach owocowych
PublikacjaW literaturze można znaleźć potwierdzenie dla tezy, że miniaturyzacja oraz automatyzacja to kluczowe elementy, które powinny być brane pod uwagę przy optymalizacji tych procedur analitycznych, w których występuje etap derywatyzacji analitów, jeśli mają być one zaliczone do grupy tzw. zielonych metodyk analitycznych. Rezultatem zastosowania takiego podejścia jest zmniejszenie ilości zużytych odczynników i tym samym, zmniejszenie...
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Polycyclic aromatic hydrocarbons in smoked fish - a critical review
PublikacjaDym wędzarniczy zawiera wśród setek składników co najmniej 100 wielopierścieniowych węglowodorów aromatycznych (WWA). Benzo{a}piren (BaP) jest uważany za wskaźnikowy, rakotwórczy WWA w wędzonych rybach. Współczesne procedury analityczne obejmujące ekstrakcję węglowodorów z matrycy, oczyszczenie, rozdzielenie metodą GC lub HPLC przy użyciu, odpowiednio, detektora MS albo FLD, umożliwiają oznaczenie indywidualnych WWA w wędzonych...
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Nonlinear planar modeling of massive taut strings travelled by a force-driven point-mass
PublikacjaThe planar response of horizontal massive taut strings, travelled by a heavy point-mass, either driven by an assigned force, or moving with an assigned law, is studied. A kinematically exact model is derived for the free boundary problem via a variational approach, accounting for the singularity in the slope of the deflected string. Reactive forces exchanged between the point-mass and the string are taken into account via Lagrange...
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Storing Matter’technique in SIMS depth profile analysis
PublikacjaSecondary ion mass spectrometry (SIMS) is a very useful technique for the analysis of layered systems. It is based on the primary ion beam sputtering of solids and mass analysis of the emitted secondary ions. A main limitation of this technique results from the direct quantitative analysis, since the ionization efficiency of a given atom is highly influenced by the neighbouring atoms at the surface. This phenomenon is known as...
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The influence of the fuel spray nozzle geometry on the exhaust gas composition from the marine 4-stroke diesel engine
PublikacjaThe paper presents experimental research on a 4-stroke, 3-cylinder, turbocharged AL25/30 Diesel engine. Research consisted in investigating the effect of the geometry of the fuel injectors on the exhaust gas composition from the engine. During measurements, the engine was operated with a regulator characteristic of a load range from 40 kW to 280 kW, made by electric water resistance. The engine was mechanically coupled to the electric...
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Capillary Electrophoresis in Determination of Low Molecular Mass Organic Acids
PublikacjaThis paper presents overviews the capabilities of capillary electrophoretic techniques to determine low molecular mass organic acids in a variety of aqueous, gas and solid samples. It mainly focuses on short - chain carboxylic acids containing one or more carboxylic groups and possibly some other functional groups (hydroxyl, keto-, amino- etc.). Finally the procedures applied to the determine the acids in different matrices are...
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Photocatalytical degradation of toluene and cyclohexane using LED illumination
PublikacjaA sol-gel process followed by hydrothermal reaction was used to prepare coupled WO3-TiO2 photocatalysts with varying amounts of WO3 in respect to TiO2 (3 mol% and 5 mol% respectively). Additionally, photocatalysts have been subjected to different calcination temperatures of 400ºC and 800ºC, which allowed us to compare how these affect photodegradation efficiency. Photocatalysts were characterized under a scanning electron microscope,...
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Novel insights into conjugation of antitumor-active unsymmetrical bisacridine C-2028 with glutathione: characteristics of non-enzymatic and glutathione S-transferase-mediated reactions
PublikacjaUnsymmetrical bisacridines (UAs) are a novel potent class of antitumor-active therapeutics. A significant route of phase II drug metabolism is conjugation with glutathione (GSH), which can be non-enzymatic and/or catalyzed by GSH-dependent enzymes. The aim of this work was to investigate the GSH-mediated metabolic pathway of a representative UA, C 2028. GSH supplemented incubations of C-2028 with rat, but not with human, liver...
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The content of conjugated linoleic acid and vaccenic acid in the breast milk of women from Gdansk and the surrounding district, as well as in, infant formulas and follow-up formulas. Nutritional recommendation for nursing women
PublikacjaFatty acids are some of the most important components of human milk. Polyunsaturated fatty acids (PUFAs) are essential nutrients required for the optimal growth and development of infants, especially the central nervous system, brain and retina. AIM: To determine the conjugated linolenic acid (CLA) and vaccenic acid (VA) content of human breast milk from mothers consuming different diets, and to compare the results with CLA and...
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Procedura przygotowania próbki do oznaczania wielopierścieniowych węglowodorów aromatycznych w produktach technicznych
PublikacjaW pracy opisano nową metodę przygotowania próbki w celu oznaczania śladowych zawartości wielopierścieniowych węglowodorów aromatycznych (WWA) w wysokowrzących produktach naftowych. Wartości granicy oznaczalności dla badanych WWA w materiałach tego typu mieściły się w przedziale od kilkudziesięciu ng/kg do poniżej dwudziestu ug/kg. Badania wykazały, że w celu oddzielenia większości zanieczyszczeń od analitów, bez ich znaczących...
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Dual-Activity Fluoroquinolone-Transportan 10 Conjugates offer alternative Leukemia therapy during Hematopoietic Cell Transplantation
PublikacjaHematopoietic cell transplantation (HCT) is often considered a last resort leukemia treatment, fraught with limited success due to microbial infections, a leading cause of mortality in leukemia patients. To address this critical issue, we explored a novel approach by synthesizing antileukemic agents containing antibacterial substances. This innovative strategy involves conjugating fluoroquinolone antibiotics, such as ciprofloxacin...
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Activated sodium percarbonate-ozone (SPC/O3) hybrid hydrodynamic cavitation system for advanced oxidation processes (AOPs) of 1,4-dioxane in water
PublikacjaHydrodynamic cavitation (HC) was employed to activate sodium percarbonate (SPC) and ozone (O3) to degrade recalcitrant 1,4-dioxane. The degradation efficiency > 99 % with a rate constant of 4.04 × 10−2 min−1 was achieved in 120 min under the optimal conditions of cavitation number (Cv) 0.27, pH 5, molar ratio of oxidant to pollutant (rox) 8, ozone dose of 0.86 g h−1 under 25 ± 2 °C with initial concentration of 1,4-dioxane 100...
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Inner-shell fragmentation of molecules into neutral fragments in high-Rydberg states induced by soft X-ray excitation with pulsed-field ionization
PublikacjaIn the present communication, we will show the results of measurements probing the production of neutral high-Rydberg fragments at the K edges of the molecules containing oxygen and nitrogen atoms. The experiments were performed at the Gas Phase beamline of the Elettra synchrotron radiation laboratory (Trieste, Italy), exploiting a combined soft X-ray excitation with pulsed-field ionization and ion time-of-flight (TOF) spectrometry...
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Pyrolysis of RDF and Catalytic Decomposition of the Produced Tar in a Char Bed Secondary Reactor as an Efficient Source of Syngas
PublikacjaOne of the technical limitations of refuse-derived fuel (RDF) pyrolysis is the high content of tar in its gas products. In order to resolve this problem, a two-stage RDF pyrolysis with a catalyst based on char from RDF pyrolysis is proposed. This paper presents the results of municipal waste pyrolysis beginning in an oven heated to 480 °C and ending with catalytic tar cracking carried out in the temperature range from 800 to 1000...
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Wykorzystanie technik instrumentalnych do oceny jakości organoleptycznej i rozróżniania destylatów rolniczych i miodów
PublikacjaPodstawowym celem pracy było opracowanie nowych metod oceny jakości organoleptycznej i rozróżniania destylatów rolniczych i miodów przy wykorzystaniu wybranych technik instrumentalnych, a następnie określenie jakie związki i w jakich ilościach występują w lotnej frakcji omawianych produktów i półproduktów oraz znalezienie różnic w składzie lotnych związków zapachowych w destylatach rolniczych różnej jakości organoleptycznej i miodach...
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Wielowymiarowa chromatografia cieczowa jako technika przygotowania próbki i oznaczania składu grupowego skomplikowanych mieszanin substancji
PublikacjaCelem ogólnym niniejszej rozprawy doktorskiej jest opanowanie problemu doboru optymalnych warunków przygotowania próbki, z uwzględnieniem rozdzielania grupowego w celu zapewnienia możliwości oznaczania zawartości wybranych analitów lub grup analitów w materiałach stanowiących skomplikowane mieszaniny bardzo wielu substancji. W części wprowadzającej pracy dokonano przeglądu i poddano krytycznej ocenie istniejące techniki i metody...
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New approach based on solid-phase extraction for the assessment of organic compound pollutions in so-called pharmaceutically pure water
PublikacjaOpisano mozliwość zastosowania nowej procedury analitycznej opartej na wykorzystaniu techniki ekstrakcji do fazy stałej (na etapie izolacji i wzbogacania analitów) do oznaczania śladowych ilości zanieczyszczeń organicznych w wodzie specjalnej czystości używanej do celów farmaceutycznych.Próbka badanej wody jest przepuszczana przez złoże sorbenta, które jest osuszane za pomocą strumienia oczyszczonego powietrza, przed etapem termicznej...
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Kapilarna chromatografia gazowa fazy nad-powierzchniowej – HS-CGC w badaniach składu lotnych produktów hydrolizy biomasy ligno-celulozowej – BMLC
PublikacjaW pracy przedstawiono sposoby obróbki biomasy ligno-celulozowej, których celem jest rozpulchnienie struktury surowca i zwiększenie dostępności struktur dla czynników hydrolizujących. Na podstawie przeglądu literatury wskazano na możliwe do zidentyfikowania związki obecne w hydrolizatach z biomasy ligno-celulozowej. Opracowano metodykę oraz zbadano możliwość oznaczania lotnych produktów hydrolizy chemicznej z wykorzystaniem techniki...
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Degradation of 1,4-dioxane by sono-activated persulfates for water and wastewater treatment applications
PublikacjaThis paper presents a hybrid advanced oxidation process (AOP) based on sonocavitational activation of persulfate (PS) for degradation of 1,4-dioxane during wastewater treatment. Application of sono-cavitation effectively convert PS to radical species demonstrating synergistic effect by increasing the reaction rate and reducing the required energy for activation. It is economically feasible and deployed alternative to the direct...
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Application of a cellular automaton to simulations of granular flow in silos
PublikacjaA cellular automaton based on a gas model of hydrodynamics was used to calculate the kinematics of non-cohesive granular materials during confined flow in a mass flow and funnel flow model silo. In the model, collisions of particles were taken into account during granular flow. In addition, a simplified automaton was used wherein granular flow was assumed as an upward propagation of holes through a lattice composed of cells representing...
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Study of the influence of thermal factors on the welding process of polyethylene gas pipelines,
PublikacjaA one-dimensional calculation scheme is proposed with the help of which it is possible to determine and set the technological parameters with the accuracy to be realized in production conditions: the temperature of the heating element and the heating time, which allows maximum mechanization of the technological operations of polyethylene gas pipelines welding. The numerical value of the coefficient of temperature for polyethylene...
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DETERMINATION OF SURFACTANTS IN ENVIRONMENTAL SAMPLES - PART I. CATIONIC COMPOUNDS = Oznaczanie Surfaktantów W Próbkach Środowiskowych. Część I. Związki Kationowe
PublikacjaCompounds from the group of cationic surfactants are widely applied in household, industrial, cleaning, disinfectant, cosmetic and pharmaceutical products as their specific properties (antimicrobial, emulsifying, anticorrosion, softening). After use, cationic surfactants are disposed to wastewater-treatment plants and finally with effluent water to surface waters due to their incomplete degradation. Moreover, they can freely circulate...
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The compositional space of exhaled breath condensate and its link to the human breath volatilome
PublikacjaBreath analysis is commonly understood to target gaseous or volatile organic compounds (VOCs) for the characterization of different pathologies. Targeted analysis is most effective if a working hypothesis can be based on a plethora of data. The recently published volatilome builds an optimal basis for organizing powerful target sets. However, the origin and pathways of biosynthesis of many VOCs are not known, which complicates...
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Obtaining of coumarone-indene resins based on light fraction of coal tar 2. Coumarone-indene resins with epoxy groups
PublikacjaCoumarone-indene resins (CIR) with carboxy groups were synthesized via cooligomerization of unsaturated compounds presented in light fraction of coal tar and its fraction boiling out within 423–463 K with the addition of such industrial monomers as styrene, maleic anhydride, glycidyl methacrylate and methacrylic acid. 2,2`-Azobis(2-methylpropionitrile) in the form of 0.2 M solution in toluene was used as an initiator. The effect...
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Synthetic Principles Determining Local Organization of Copolyesters Prepared from Lactones and Macrolactones
PublikacjaA highly effective and facile technique for catalytic ring-opening copolymerization (cROP) of lactones viz. ε- caprolactone and ε-decalactone with ω-pentadecalactone is being described. The reactions were mediated by Zn- and Cabased tridentate Schiff base complexes and benzyl alcohol as initiator. The catalysts were successfully employed for the preparation of numerous block and random copolymers. To unravel the composition of...
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Synthesis, Antimicrobial and Mutagenic Activity of a New Class of d-Xylopyranosides
PublikacjaEight N-[2-(2′,3′,4′-tri-O-acetyl-α/β-d-xylopyranosyloxy)ethyl]ammonium bromides, a new class of d-xylopyranosides containing a quaternary ammonium aglycone, were obtained. Their complete structure was confirmed using NMR spectroscopy (1H, 13C, COSY and HSQC) and high-resolution mass spectrometry (HRMS). An antimicrobial activity against fungi (Candida albicans, Candida glabrata) and bacteria (Staphylococcus aureus, Escherichia...
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Determination of dryout localization using a five-equation model of annular flow for boiling in minichannels
PublikacjaDetailed studies have suggested that the critical heat flux in the form of dryout in minichannels occurs when the combined effects of entrainment, deposition, and evaporation of the film make the film flow rate go gradually and smoothly to zero. Most approaches so far used the mass balance equation for the liquid film with appropriate formulations for the rate of deposition and entrainment respectively. It must be acknowledged...
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Superhydrophobic sponges based on green deep eutectic solvents for spill oil removal from water
PublikacjaThe paper described a new method for crude oil-water separation by means of superhydrophobic melamine sponges impregnated by deep eutectic solvents (MS-DES). Due to the numerous potential of two-component DES formation, simple and quick screening of 156 non-ionic deep eutectic solvents using COSMO-RS (Conductor-like Screening Model for Real Solvents) computational model was used. DES which were characterized by high solubility...
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Validation of atmospheric aerosols sampling in equivalent fractions
PublikacjaPhysical properties and chemical composition of atmospheric aerosols are of extreme importance, in many fields of interest, namely the Environment even with legal implications. Atmospheric sampling poses inherent problems with consequent demands on sampling optimisation, with sampling strategies requiring validation. In this work a study was conducted aiming at validating the collection of atmospheric aerosols in equivalent fractions....
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Kontrola jakości i analityka techniczna [Moduł dyscyplinarny, grupa B]
Kursy OnlineProwadzący: Dr hab. inż. Grzegorz Boczkaj prof. PG Terminy realizacji: 25 marca, 1 kwietnia, 15 kwietnia, 22 kwietnia, 13 maja w godz. od 09:15 do 12-tej /zajęcia online Tematyka realizowanego przedmiotu obejmuje: Pobieranie próbek do badań (on-line, off-line, at-line, in-line) Przygotowanie próbek do badań (techniki i przykłady metodyk dla próbek stałych, ciekłych i gazowych) Techniki i wybrane metodyki klasyczne (miareczkowanie,...
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A model of liquid film breakdown formed due to impingement of a two-phase jet on a horizontal surface
PublikacjaThe present work aims to provide an explanation to the phenomenon of breakdown of the thin liquid film created by impinging two-phase, liquid-gas jet. Existing in the literature models describe merely thebreakdown of single phase liquid films. The model presented here is based on examination of mass and energy equations under the applied criterion of the minimum of total energy. That allows to determinethe minimum thickness of...
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Current trends in analytical strategies for the determination of polybrominated diphenyl ethers (PBDEs) in samples with different matrix compositions – Part 2: New approaches to PBDEs determination
PublikacjaHerein, we review the latest technical solutions for polybrominated diphenyl ethers (PBDEs) determination in samples with different matrix compositions, largely focusing on gas chromatography but also presenting some liquid chromatography–based solutions. Moreover, we discuss the use of diverse sample injection techniques while considering the advantages and disadvantages of each injection system in the context of PBDEs analysis....
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Anti-cancer effect of Rumex obtusifolius in combination with arginase/nitric oxide synthase inhibitors via downregulation of oxidative stress, inflammation, and polyamine synthesis
PublikacjaCancer continues to be a leading cause of death worldwide, making the development of new treatment methods crucial in the fight against it. With cancer incidence rates increasing worldwide, ongoing research must focus on identifying new and effective ways to prevent and treat the disease. The combination of herbal extracts with chemotherapeutic agents has gained much interest as a novel strategy to combat cancer. Rumex obtusifolius...
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Surface sediments pollution due to shipwreck s/s “Stuttgart”: a multidisciplinary approach
PublikacjaShipwrecks may pose a serious source of pollution for marine ecosystems. For this reason, it is of great importance to perform a marine ecosystem risk assessment due to plausible wreck presence. One of elements of such an assessment is determining the state of the environment in the vicinity of the wreck. In the work presented, the results of studies on surface sediments samples collected around the s/s Stuttgart ship sunk during...
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Fabrication and photoactivity of organic-inorganic systems based on titania nanotubes and PEDOT containing redox centres formed by different Prussian Blue analogues
PublikacjaHerein, the heterojunction composed of an inorganic substrate: ordered hydrogenated titania nanotubes (H-TiO2NTs) and a deposited organic film: poly(3,4-ethylenedioxythiophene) (PEDOT) is reported. The conducting polymer is modified with different transition metal haxacyanoferrates (Mehcf), wherein as metal: copper, iron, cobalt and nickel are introduced. The presence of various metal centres provides characteristic redox activity...
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Usuwanie lotnych związków organicznych z fazy gazowej z zastosowaniem cieczy jonowych
PublikacjaUżycie cieczy jonowej do usuwania LZO jest limitowane przez rozpuszczalność usuwanych związków. Dane literaturowe sugerują, że ciecze jonowe jako faza membranowa mogą być z powodzeniem stosowane do selektywnej separacji węglowodorów aromatycznych z mieszanin węglowodorów aromatycznych i alifatycznych. Przedmiotem pracy było kompleksowe opisanie procesu separacji LZO z fazy gazowej z uwzględnieniem czynników wpływających na efektywność...
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Exergy analysis of a negative CO2 emission gas power plant based on water oxy-combustion of syngas from sewage sludge gasification and CCS
PublikacjaA power cycle with water-injected oxy-combustion (water cycle) is investigated by exergy analysis. It is fueled with syngas (aka. producer gas) from gasification of sewage sludge. The cycle is equipped with a spray-ejector condenser (SEC). CO2 is separated and compressed for transportation and storage. The net delivered electric power is 31% of the fuel exergy. The task efficiency is 39% when the flue gas bleed to gasification...
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Organotin Compounds: Environmental Fate and Analytics
PublikacjaFor more than 50 years, organotin compounds have been used in almost all sectors of industry. As a consequence, specific environmental compartments are affected by the emission of these xenobiotics. The emissions of organotin compounds into the environment from paints used in the shipbuilding industry for ship hull coating, and from the plant protection products are particularly significant.In 2001, the IMO introduced a global...
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The impact of addition of olive oil on thermal degradation of refined rapeseed oil
PublikacjaFats are an important component of the everyday diet and have a significant impact on the proper functioning of human organism. However, during the process of frying chemical transformations take place in the oil; hence fats characterised by high oxidative stability should be given preference. The aim of this work was to determine the quality of rapeseed oil, blended oil, and refined olive oil, all sourced from the domestic market....
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Monitoring of n-butanol vapors biofiltration process using an electronic nose combined with calibration models
PublikacjaMalodours, by definition, are generally unpleasant, nuisance smells that are a mixture of volatile chemical compounds which can be perceptible even at low concentrations. Due to the more frequent occurrence of odour nuisance associated with the odour sensations, and thus the need to remove them from the air, deodorization techniques are commonly used. Biofiltration is one of the methods of reducing odorants in the air stream. In...
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Influence of hydrogen on a nanocrystalline high-entropy oxide with application potential in hydrogen technologies
PublikacjaThe influence of hydrogen on a (Co0⋅2Cu0⋅2Mg0⋅2Ni0⋅2Zn0.2)O HEO with a rock salt structure was studied. Two forms of this HEO (nanopowder and sinter) were prepared via mechanochemical synthesis (MS) and additional heat treatment, respectively. The chemical stability of the synthesized samples in a pure hydrogen atmosphere was investigated using Sievert’s technique. High-pressure hydrogenation at 250 ◦C had no noticeable effect...
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Validation of atmospheric aerosols parallel sampling in a multifold device
PublikacjaIn this work, particulate matter was collected using an active sampling system consisting of a PM10 (<10 μm) inlet coupled to a multifold device containing six channels, connected to a vacuum pump. Each channel was equipped with a filter holder fitted with adequately chosen filters. The system was fixed on a metallic structure, which was placed on the roof of the laboratory building, at the Faculty of Sciences, in Lisbon. Sampling...
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The influence of Be addition on the structure and thermal properties of alkali-silicate glasses
PublikacjaBe-Na-(Li)-Si oxide glasses containing up to 15 mol% of BeO were prepared. Their structure was characterized by X-ray powder diffraction and Raman as well as infrared spectroscopic techniques, while their chemical compositions were examined by Inductively Coupled Plasma Optical Emission Spectrometry. All materials were found to be amorphous and contain Al contaminations from minor dissolution of the alumina crucibles. The results...
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Preparation, characterization and anti-colitis activity of curcumin-asafoetida complex encapsulated in turmeric nanofiber
PublikacjaUlcerative colitis (UC) is a main form of inflammatory bowel disease (IBD). Asafoetida (ASF) and turmeric have traditionally been used for the treatment of various inflammatory diseases, including UC, because ASF is rich in sulfur compounds and turmeric contains curcumin (CUR). Turmeric nanofiber (TNF), the modified cell wall component of turmeric is considered to play important role in the human diet, health and can be used as...
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Aminy w środowisku - niebezpieczeństwo czy jego naturalny element?
PublikacjaA lot of amines and their compounds are used in the industry. First of all they are used for the preparation of medicines, plant protection agents, fertilizers and plastics. Amines are also released into the environment as a result of natural biological processes such as the release of certain species of flowers to lure insects, and also biochemical breakdown of amino acids in the protein during decomposition of organic matter. Unfortunately,...
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Optical and chemical characterization of thin TiNx films deposited by DC-magnetron sputtering
PublikacjaThin titanium nitride (tinx) films were deposited on silicon substrates by means of a reactive dc-magnetron plasma. Layers were synthesized under various conditions of discharge power and nitrogen flows in two operation modes of the magnetron (the so-called "balanced" and "unbalanced" modes). The optical constants of the tinx films were investigated by spectroscopic ellipsometry (se). X-ray photoelectron spectroscopy (xps) was...
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Isotope-labeled substances in analysis of persistent organicpollutants in environmental samples
PublikacjaUltratrace analysis of persistent organic pollutants (POPs) in environmental samples requires very sophisticated methods for both sample preparation and instrumental analysis. The complex matrix requires a multi-stage procedure. Each stage is a potential source of error, as a consequence of which the final result of analysis could be a source of misinformation rather than information. The individual stages and the procedure as...
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In Vitro Studies on the Relationship Between the Antioxidant Activities of Some Berry Extracts and Their Binding Properties to Serum Albumin
PublikacjaThe aim of this study was to investigate the possibility to use the bioactive components from cape gooseberry (Physalis peruviana), blueberry (Vaccinium corymbosum), and cranberry (Vaccinium macrocarpon) extracts as a novel source against oxidation in food supplementation. The quantitative analysis of bioactive compounds (polyphenols, flavonoids, flavanols, carotenoids, and chlorophyll) was based on radical scavenging spectrophometric assays...
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Homoisocitrate dehydrogenase from Candida albicans: properties, inhibition, and targeting by an antifungal pro-drug
PublikacjaThe LYS12 gene from Candida albicans, coding for homoisocitrate dehydrogenase was cloned and expressed as a His-tagged protein in Escherichia coli. The purified gene product catalyzes the Mg2+- and K+-dependent oxidative decarboxylation of homoisocitrate to alpha-ketoadipate. The recombinant enzyme demonstrates strict specificity for homoisocitrate. SDS-PAGE of CaHIcDH revealed its molecular mass of 42.6±1 kDa, while in size-exclusion...