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Search results for: ANTI-CAS9 PROTEIN
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Failures and a concept of corrosion protection system for spiral classifiers at KGHM Polska Miedź S.A. Ore Concentration Plant
PublicationThe Ore Concentration Plant, where the process of flotation is carried out as well as the final production of copper concentrate, plays a key role in the entire production line of KGHM Polska Miedź S.A. Majority of operations related to the run-of-mine preparation to copper flotation enrichment are carried out in a water environment. The maintaining of production process continuity requires to pursue minimisation of many production...
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The Influence of the Co-Dopant Dexamethasone Phosphate on the Electrodeposition Process and Drug-Release Properties of Polypyrrole-Salicylate on Iron
PublicationIn this work, the influence of incorporation of two anti-inflammatory drugs dexamethasone phosphate and sodium salicylate on the electrodeposition process of polypyrrole (PPy) film on iron has been studied. The incorporation of the agents into PPy has been carried out in a one-step electropolymerization process on oxidizable iron. The role of each drug agent during the deposition process has been discussed. The morphological and...
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Toxoplasma gondii recombinant antigens as tools for serodiagnosis of human toxoplasmosis: current status of studies
PublicationToxoplasma gondii is a parasitic protozoan which is the cause of toxoplasmosis. Although human toxoplasmosis in healthy adults is usually asymptomatic, serious disease can occur in the case of congenital infections and immunocompromised individuals. Furthermore, despite the exact recognition of its etiology, it still presents a diagnostic problem. Diagnosis of toxoplasmosis is mainly based on the results of serological tests detecting...
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Influence of permanent magnetic field on wear performance of dry sliding contacts
PublicationResults of experimental studies concerning the influence of permanent magnetic field on wear of dry sliding contact are presented.It was found that magnetic field of horizontal orientation is causing temporary reduction of material's hardness. It also facilitates and accelerates removal process of wear particles from the contact zone.Formation of bulges, consisting of accumulated and compacted anti-ferromagnetic material produced...
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A Model-Based Improved Control of Dissolved Oxygen Concentration in Sequencing Wastewater Batch Reactor
PublicationBiochemical processes at wastewater treatment plant are complex, nonlinear, time varying and multivariable. Moreover, relationships between processes are very strong. One of the most important issues is exerting proper control over dissolved oxygen levels during nitrification phase. This parameter has a very large impact on activity of microorganisms in activated sludge and on quality of pollution removal processes. Oxygen is supplied...
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Analysis of hydrodynamic pressure fields of motorboats and pontoons in shallow water
PublicationThe article presents the results of calculations of the pressure fields generated by a motorboat at the bottom of a shallow sea. Calculations were made using the boundary elements method (BEM), arranged on the surface of the boat and the bottom of the sea. This method is described in [3], and applied on a free surface linearized boundary condition. Results for four different lengths of motorboats, from 2.85 m to 9.5 m, sea depth...
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Evaluation of organic coating systems for corrosion protection of industrial equipment using impedance spectroscopy
PublicationThe high economic impact of corrosion can be reduced by enhanced corrosion management and prevention based on appropriate means. This applies in particular to the protection of structures with organic protective coatings. Steel is subject to corrosion that reduces its service life and leads to destruction in most severe cases. The application of paints to metallic objects for corrosion control has been known for a long time and...
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Design, synthesis and biological evaluation of novel N-phosphorylated and O-phosphorylated tacrine derivatives as potential drugs against Alzheimer’s disease
PublicationIn this work, we designed, synthesised and biologically investigated a novel series of 14N- and O-phosphorylated tacrine derivatives as potential anti-Alzheimer’s disease agents. In the reaction of 9-chlorotacrine and corresponding diamines/aminoalkylalcohol we obtained diamino and aminoalkylhydroxy tacrine derivatives. Next, the compounds were acid to give final products 6–13 and 16–21 that were characterised by 1H, 13 C, 31P...
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FRP-based reinforcement coatings of steel with application prospects in ships and offshore structures: a review
PublicationLatest research on novel FRP-based anti-corrosion structural coatings (for enhancing structural capacity and strengthening the coating layer) is discussed with application prospects for ships and offshore structures. In the marine environment, structures constantly face corrosion and fatigue cracks. Combining this with high operational and wave loads, it might cause a structural collapse. Recently, polymer composites have been...
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Malignant lymphoma, large B-cell, diffuse, centroblastic, NOS - Female, 84 - Tissue image [9300729527452571]
Open Research DataThis is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Ferromagnetic nanoparticles imaging by means of Magnetic Force Microscopy
Open Research DataFerromagnetic nanoparticles can be used as building blocks for advanced thin film magnets, and can also be used in data storage and biomedical technologies. Nano-crystalline ferrites with the chemical formula NixZn (1 - x) Fe2O4, where x = 0, 0.2, 0.4, 0.6, 0.8, 1.0 show anti-corrosion properties and suppress electromagnetic interference, in the case...
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Molecular identification and genotyping of Staphylococci: genus, species, strains, colnes, lineages, and interspecies exchanges
PublicationStaphylococci are increasingly recognized as etiological agents of many opportunistic human and animal infections, emphasizing the need for a rapid and accurate identification, even to a genotypical level of these bacteria. In the recent years, there has been a significant progress in typing and phylogenetic study of Staphylococcus species. Here, we describe molecular methods used in taxonomy as well as staphylococci characterization....
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Role of Human UDP-Glucuronosyltransferases in the Biotransformation of the Triazoloacridinone and Imidazoacridinone Antitumor Agents C-1305 and C-1311 : Highly Selective Substrates for UGT1A10
Publication5-Diethylaminoethylamino-8-hydroxyimidazoacridinone, C-1311 (NSC-645809), is an antitumor agent shown to be effective against breast cancer in phase II clinical trials. A similar compound, 5-dimethylaminopropylamino-8-hydroxytriazoloacridinone, C-1305, shows high activity against experimental tumors and is expected to have even more beneficial pharmacological properties than C-1311. Previously published studies showed that these...
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THE GENE CLONING, OVEREXPRESSION, PURIFICATION AND BIOCHEMICAL CHARACTERISATION OF A NEW COLD-ADAPTED β–GALACTOSIDASE FROM ARTHROBACTER SP. VII-4
PublicationINTRODUCTION. β-Galactosidase [EC 3.2.1.23] is an enzyme that catalyzes the hydrolysis of O-glycosidic linkages in galactosides. It is commercially used in dairy industry for the production of milk with reduced lactose content. Potentially, the best method for lactose removal under cooling conditions should be carried out with a cold-adapted enzyme. AIM. The aim of this study was to determine the taxonomic affiliation of the isolate...
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Diamond Nanofilm Normalizes Proliferation and Metabolism in Liver Cancer Cells
PublicationPurpose: Surgical resection of hepatocellular carcinoma can be associated with recurrence resulting from the degeneration of residual volume of the liver. The objective was to assess the possibility of using a biocompatible nanofilm, made of a colloid of diamond nanoparticles (nfND), to fill the side after tumour resection and optimize its contact with proliferating liver cells, minimizing their cancerous transformation. Methods:...
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Biofilm bakteryjny uropatogennych szczepów E. coli Dr+ jako czynnik indukujący przewlekłość zakażeń dróg moczowych ograniczający ich leczenie
PublicationZakażenia dróg moczowych (ZUM) stanowią jedne z najczęściej występujących infekcji bakteryjnych, dotykających każdego roku miliony osób na świecie. Problematyka tych zakażeń wynika z ich przewlekłości i nawrotów, pomimo stosowania terapii antybiotykowej oraz ciągle wzrastającej lekooporności uropatogenów je wywołujących. Dominującym czynnikiem etiologicznym ZUM są uropatogenne szczepy E. coli (UPECs), wykazujące zdolność do adhezji,...
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Aminy w środowisku - niebezpieczeństwo czy jego naturalny element?
PublicationA lot of amines and their compounds are used in the industry. First of all they are used for the preparation of medicines, plant protection agents, fertilizers and plastics. Amines are also released into the environment as a result of natural biological processes such as the release of certain species of flowers to lure insects, and also biochemical breakdown of amino acids in the protein during decomposition of organic matter. Unfortunately,...
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Targeting shelterin proteins for cancer therapy.
PublicationAs a global health challenge, cancer prompts continuous exploration for innovative therapies that are also based on new targets. One promising avenue is targeting the shelterin protein complex, a safeguard for telomeres crucial in preventing DNA damage. The role of shelterin in modulating ataxia- telangiectasia mutated (ATM) and ataxia-telangiectasia and Rad3-related (ATR) kinases, key players in the DNA damage response (DDR),...
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Biofilm Production and Presence of ica and bap Genes in Staphylococcus aureus Strains Isolated from Cows with Mastitis in the Eastern Poland
PublicationThe aim of the study was phenotypic and genotypic analysis of 132 S. aureus strains isolated from mastitis in eastern Poland in respect to their biofilm formation ability. The analysis of the size polymorphism of fragment X in the gene encoding protein A (spa) revealed high genetic differentiation of the analyzed group of isolates. The ability of biofilm formation by the isolates was tested using two phenotypic methods. The Congo...
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Current harmonic controller in multiple reference frames for series active power filter integrated with 18-pulse diode rectifier
PublicationThe paper presents the control system and selected results of experimental tests of the AC/DC power converter consisting of an 18-pulse diode rectifier based on coupled reactors and a serial active power filter. Proportional integral controllers with decoupling components are implemented in multiple reference frames for selective line current harmonic suppression. The regulator is provided with a backtracking anti-windup mechanism...
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What is important for you makes you think about the pandemic differently: moral foundations, pandemic-related fears and convictions (Lo que cada uno consideramos importante nos hace reflexionar sobre la pandemia de forma distinta: fundamentos morales, temores y convicciones sobre la pandemia)
PublicationBuilding on the Moral Foundations Theory and findings regarding the linkage of values, convictions and beliefs, the aim of the study was to compare people displaying various constellations of moral foundations regarding their tolerance of ambiguity, fear of COVID-19 (FCV), endorsement of COVID-19 conspiracy theories and the extent to which they believed in the effectiveness of five COVID-19 preventive measures. This study was...
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Detection and Mitigation of GPS Spoofing Based on Antenna Array Processing
PublicationIn this article authors present an application of spatial processing methods for GPS spoofing detection and mitigation. In the first part of this article, a spoofing detection method, based on phase delay measurements, is proposed. Accuracy and precision of phase delay estimation is assessed for various qualities of received signal. Spoofing detection thresholds are determined. Efficiency of this method is evaluated in terms of...
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Two-Step Model Based Adaptive Controller for Dissolved Oxygen Control in Sequencing Wastewater Batch Reactor
PublicationDissolved Oxygen (DO) concentration is a crucial parameter for efficient operation of biological processes taking place in the activated sludge Wastewater Treatment Plant (WWTP). High-quality DO control is difficult to achieve because of complex nonlinear behavior of the plant and substantial influent disturbances. A method to improve the Direct Model Reference Adaptive Control (DMRAC) technology in application to DO tracking for...
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Analysis of Airport Runoff Waters
PublicationA particularly important facet of airport operation is the impact of the pollution caused byrunoff waters. Runoff waters at an airport may contain relatively high concentrations of differentcontaminants resulting from the various aspects of its operation: de/anti-icing operations,washing and cleaning operations, spills of fuel and lubricants, exhaust fumes, and weed removal.The pollution caused by airport operations affects soil,...
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Influence of silver nanoparticles addition on antibacterial properties of PEO coatings formed on magnesium
PublicationMagnesium is a biodegradable material and thus could be a choice for bone fixation devices and implants with a specific purpose. This study aims to enhance the anti-corrosive, biocompatible, and antibacterial properties on magnesium-based materials through ceramic coatings formation. To achieve this the silicate-based electrolyte was used to create of Plasma Electrolytic Oxidation (PEO) coatings. During investigation the bioactive...
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Global Complex Roots and Poles Finding Algorithm in C × R Domain
PublicationAn algorithm to find the roots and poles of a complex function depending on two arguments (one complex and one real) is proposed. Such problems are common in many fields of science for instance in electromagnetism, acoustics, stability analyses, spectroscopy, optics, and elementary particle physics. The proposed technique belongs to the class of global algorithms, gives a full picture of solutions in a fixed region ⊂ C × R and...
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Monoclonal gammopathy of undetermined significance, NOS - Male, 69 - Tissue image [11290630017293191]
Open Research DataThis is the histopathological image of HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Waldenstrom macroglobulinemia - Female, 50 - Tissue image [11290630017291461]
Open Research DataThis is the histopathological image of HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Monoclonal gammopathy of undetermined significance, NOS - Male, 69 - Tissue image [11290630017291931]
Open Research DataThis is the histopathological image of HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Waldenstrom macroglobulinemia - Female, 50 - Tissue image [11290630017282841]
Open Research DataThis is the histopathological image of HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Monoclonal gammopathy of undetermined significance, NOS - Male, 69 - Tissue image [11290630017291571]
Open Research DataThis is the histopathological image of HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Influence of the ionic strength on the amyloid fibrillogenesis of hen egg white lysozyme
PublicationThe study investigates the role of the electrostatic interactions in the fibrillation of the hen egg white lysozyme (HEWL). In order to achieve this aim the influence of the cations Na+, Mg2+ and Al3+ on the amyloid fibril formation and amorphous aggregation was tested. The amyloids are formed in the solution without added salt but the Thioflavin T fluorescence gives the false-negative result. In these conditions, the HEWL fibrils...
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Comparison of the main bioactive compounds and antioxidants in garlic and white and red onion after treatment protocols
PublicationPolski czosnek, biała i czerwona cebula była blanszowana, gotowana, smażona i poddawana działaniom promieniowania mikrofalowego. Następnie były w nich oznaczane m.in. takie związki jak polifenole, flawonoidy, kwas ascorbinowy i aktywne antyoksydanty. Udowodniono, że blanszowanie, smażenie i poddawanie działaniu promieniowania mikrofalowego nie wpływa znacząco na zmiejszenie zawartości badanych związków w czosnku i cebuli. Wyznaczono...
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Ligand-Modified Boron-Doped Diamond Surface: DFT Insights into the Electronic Properties of Biofunctionalization
PublicationWith the increasing power of computation systems, theoretical calculations provide a means for quick determination of material properties, laying out a research plan, and lowering material development costs. One of the most common is Density Functional Theory (DFT), which allows us to simulate the structure of chemical molecules or crystals and their interaction. In developing a new generation of biosensors, understanding the nature...
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The Boltzmann sequence-structure channel
PublicationWe rigorously study a channel that maps binary sequences to self-avoiding walks in the two-dimensional grid, inspired by a model of protein statistics. This channel, which we also call the Boltzmann sequence-structure channel, is characterized by a Boltzmann/Gibbs distribution with a free parameter corresponding to temperature. In our previous work, we verified experimentally that the channel capacity has a phase transition for...
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Long‐time scale simulations of virus‐like particles from three human‐norovirus strains
PublicationThe dynamics of the virus like particles (VLPs) corresponding to the GII.4 Houston, GII.2 SMV, and GI.1 Norwalk strains of human noroviruses (HuNoV) that cause gastroenteritis was investigated by means of long-time (about 30 μs in the laboratory timescale) molecular dynamics simulations with the coarse-grained UNRES force field. The main motion of VLP units turned out to be the bending at the junction between the P1 subdomain (that...
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The biological role of prolyl oligopeptidase and the procognitive potential of its peptidic inhibitors from food proteins
PublicationProlyl oligopeptidase (POP) is a conserved serine protease belonging to proline-specific peptidases. It has both enzymatic and non-enzymatic activity and is involved in numerous biological processes in the human body, playing a role in e.g., cellular growth and differentiation, inflammation, as well as the development of some neurodegenerative and neuropsychiatric disorders. This article describes the physiological and pathological...
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FROM HARVESTING TO DISTILLATION - EFFECT OF ANALYTICAL PROCEDURES ON THE YIELD AND CHEMICAL COMPOSITION OF RHODODENDRON TOMENTOSUM (LEDUM PALUSTRE) ESSENTIAL OIL
PublicationRhododendron tomentosum possesses the anti-inflammatory, analgesic and antimicrobial properties, determined by the chemical composition of its essential oil. The effects of place (Miszewko, Lubichowo) and time of harvesting (June, November) as well as drying (air-drying, oven-drying, freeze-drying) and isolation (in Deryng, Clevenger and Likens-Nickerson apparatus) procedures on the yield and quality of R. tomentosum essential...
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Preparation and evaluation of 1,3-alternate 25,27-bis-(pentafluorobenzyloxy)-26,28-bis-(3-propyloxy)-calix[4]arene-bonded silics gel high performance liquid chromatography stationary phase
PublicationA 1,3-alternate 25,27-bis-(pentafluorobenzyloxy)-26,28-bis-(3-propyloxy)-calix[4]arene-bonded silica gel stationary phase (CalixBzF10) was synthesized, structurally characterized, and used as a selector in liquid chromatography. The selectivity study of this phase was done by using fluorine-containing compounds (fluorobenzenes, fluoro-pyrimidine bases), as well as non-fluorinated analytes (non-steroidal anti-inflammatory drugs,...
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RESEARCH ON ORGANIC COATINGS DESIGNED FOR UNDERWATER APPLICATIONS
PublicationUnderwater steel structures require periodic maintenance. In the case of vessels, anti-corrosion works are carried out in the shipyard, where very good conditions for applying organic protective coatings can be provided. Very good surface preparation can be obtained by the use of abrasive blasting. The well-prepared metal surface is free from impurities (particularly inorganic salts). Suitable conditions for the application and...
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Low-Barrier Hydrogen Bond Determines Target-Binding Affinity and Specificity of the Antitubercular Drug Bedaquiline
PublicationThe role of short strong hydrogen bonds (SSHB) in ligand-target binding remains largely unexplored, thereby hin- dering a potentially important avenue in the rational drug de- sign. Here, we investigate the interaction between bedaquiline (Bq), a potent anti-tuberculosis drug, and the mycobacterial ATP synthase, to unravel the role of a specific hydrogen bond to a conserved acidic residue in the target affinity and specificity....
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The electrochemical response to BDD electrode functionalization with CD147 receptor
Open Research DataThis dataset contains the results of the electrochemical impedance spectroscopy (EIS) measurements of the boron-doped diamond (BDD) electrode functionalization with CD147 receptor. The functionalized electrode was then used for the electrochemical detection of the SARS-CoV-2 virus S1 protein.
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The electrochemical response to BDD electrode functionalization with ACE2 receptor
Open Research DataThis dataset contains the results of the electrochemical impedance spectroscopy (EIS) measurements of the boron-doped diamond (BDD) electrode functionalization with ACE2 receptor. The functionalized electrode was then used for the electrochemical detection of the SARS-CoV-2 virus S1 protein.
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The electrochemical response to BDD electrode functionalization with IgG2B receptor
Open Research DataThis dataset contains the results of the electrochemical impedance spectroscopy (EIS) measurements of the boron-doped diamond (BDD) electrode functionalization with IgG2B receptor. The functionalized electrode was then used for the electrochemical detection of the SARS-CoV-2 virus S1 protein.
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Does the chemical modification of Nystatin A1 affect the drug's ability to overcome the multidrug resistance of fungi?
PublicationAlthough the contemporary medicine keeps moving forward, disseminated infections caused by fungal pathogens are an emerging challenge. The dramatic rise of fungal diseases, especially the most life-threatening systemic mycoses is associated with a permanently growing number of immunodeficient patients. Undoubted difficulties in the treatment of fungal infections are caused by lack of highly effective and selective antifungal drugs,...
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Structural features responsible for control of the eukaryotic GlcN-6-P synthase activity
PublicationGlucosamine-6-phosphate (GlcN-6-P) synthase is responsible for catalysis of the first and practically irreversible step in hexosamine metabolism. As UDP-GlcNAc, the final product of the hexosamine path, constitutes one of the essential substrates for assembly of bacterial and fungal cell walls, the enzyme is an interesting target for antimicrobial therapy.The structure of E. coli enzyme, known since 2001, forms a dimer of two identical...
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Regulated assembly of lipopolysaccharide and sensing of its alterations in Escherichia coli
PublicationThis thesis describes mechanism of the regulation of the transcription of the rpoE gene encoding an essential RNA polymerase subunit in Escherichia coli. The RpoE regulates extracytoplasmic stress response regulon and is required to initiate transcription of genes, whose products are involvedin the folding of periplasmic proteins and synthesis and transport of outer membrane components. The transcriptional regulation of the rpoE...
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Regulation of LPS assembly via controlled proteolysis and sensing of LPS stress in Escherichia coli
PublicationLipopolysaccharide (LPS) is a complex glycolipid, essential for the bacterial viability and along with phospholipids, it constitutes the major amphiphilic component of outer membrane (OM) in most of the Gram-negative bacteria, including Escherichia coli. LPS molecules confer an effective permeability barrier to the OM and play a crucial role in bacteria-environment and -host interactions. The synthesis and accumulation of this...
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Electronic noses: Powerful tools in meat quality assessment
PublicationMain factors that are considered by consumers when choosing meat products are colour and aroma, of which the latter is a more reliable indicator of quality. However, a simple sensory evaluation of hedonistic qualities is often not sufficient to determine whether protein is past its shelf life, and consumption of spoiled meat can lead to serious health hazards. Some volatile compounds can be used as spoilage indicators, and so a...
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MRM–MS of marker peptides and their abundance as a tool for authentication of meat species and meat cuts in single-cut meat products
PublicationThe abundance of protein markers in different types of meat cuts was explored in the context of authentication of raw meat (pork, beef and chicken) and processed meat products. Peptides originating from myoglobin (Mb) and myosin (My) were analyzed using multiple reaction monitoring mass spectrometry (MRM–MS). Analytical protocol was optimized for good repeatability (CV < 10%) and high sensitivity. The MS signal intensity of Mb...