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Search results for: ESSENTIAL MATRIX
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Matrix solid-phase dispersion with sand in chromatographic analysis of essential oils in herbs
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Can matrix solid phase dispersion (MSPD) be more simplified? Application of solventless MSPD sample preparation method for GC–MS and GC–FID analysis of plant essential oil components
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A framework for risk matrix design: A case of MASS navigation risk
PublicationRisk matrix, a tool for visualizing risk assessment results, is essential to facilitate the risk communication and risk management in risk-based decision-making processes related to new and unexplored socio-technical systems. The use of an appropriate risk matrix is discussed in the literature, but it is overlooked for emerging technologies such as Maritime Autonomous Surface Ships (MASS). In this study, a comprehensive framework...
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Depth Determination Accuracy of the Modified Prony Method in a Swath Mapping Application
PublicationThis article presents the performance of the modified Prony method in a swath mapping application. Depth determination accuracy is assessed by processing raw signal acquired by an EdgeTech 6205 swath bathymetry system over flat seafloor. An updated version of the method, proposed previously by the authors, is used to determine the number of signal echoes. The number of signal echoes is essential for performing the low-rank approximation...
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The accessibility, necessity, and significance of certified reference materials for total selenium content and its species to improve food laboratories' performance
PublicationMicronutrients are one of the most important groups of nutrients that our body needs daily in trace amounts to tackle deficiencies. Selenium (Se) is a mineral that occurs naturally in foods and is an essential component of selenoproteins that support the healthy functioning of the human body. Therefore, monitoring dietary Se concentrations must be a higher priority to meet daily intakes. Fulfillment can be addressed through applying...
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Polyurethane Nanocomposites Containing Reduced Graphene Oxide, FTIR, Raman, and XRD Studies
PublicationRecently, graphene and other graphene-based materials have become an essential part of composite science and technology. Their unique properties are not only restricted to graphene but also shared with derivative compounds like graphene oxide, reduced graphene oxide, functionalized graphene, and so forth. One of the most structurally important materials, graphene oxide (GO), is prepared by the oxidation of graphite. Though removal...
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Correlation–polarization effects in electron/positron scattering from acetylene: A comparison of computational models
PublicationDifferent computational methods are employed to evaluate elastic (rotationally summed) integral and differential cross sections for low energy (below about 10 eV) positron scattering off gas-phase C2H2 molecules. The computations are carried out at the static and static-plus-polarization levels for describing the interaction forces and the correlation–polarization contributions are found to be an essential component for the correct...
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Analysis and bioanalysis: an effective tool for data collection of environmental conditions and processes
PublicationReliable information that allows us to estimate the state of the environment and to forecast changes in the ecosystem are constantly required. Increasing environmental consciousness and dynamic development of analytical techniques are the main reasons for determining the wide range of pollutants occurring at very low concentrations in complex matrix samples. The presence and concentration of many of those pollutants in the environment...
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Review of the determination of modern used pesticides in water samples
PublicationThe increasingly widespread application of pesticides causes that larger amounts of them are entering the environment. Their presence in sources of drinking water, essential to life, is a particular danger. For these reasons it is important to monitor and analyse pesticide residues in the environment. The analysis of environmental samples for the presence of pesticides is very difficult. Firstly, the processes involved in sample...
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Microscopic and Spectroscopic Imaging and Thermal Analysis of Acrylates, Silicones and Active Pharmaceutical Ingredients in Adhesive Transdermal Patches
PublicationDermal or transdermal patches are increasingly becoming a noteworthy alternative ascarriers for active pharmaceutical ingredients (APIs), which makes their detailed physicochemical evaluation essential for pharmaceutical development. This paper demonstrates mid-infrared (FTIR) and Raman spectroscopy with complementary microscopic methods (SEM, optical and confocal Raman microscopy) and differential scanning calorimetry...
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Optimized Space Vector Modulation strategy for five phase voltage source inverter with third harmonic injection
PublicationThis paper presents a simple and an effective SVM algorithm for five-phase Voltage-Source Inverters with the possibility to control independently the voltage vectors for fundamental and auxiliary orthogonal subspaces. The essential benefit is that output voltage is generated using only four active voltage vectors with limited numbers of switching. In the proposed solution, four active vectors are arbitrary chosen, independent of...
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Influence of Surface-Modified Montmorillonite Clays on the Properties of Elastomeric Thin Layer Nanocomposites
PublicationIn recent years, polyurethane nanocomposites have attracted more attention due to the massive demand for materials with increasingly exceptional mechanical, optical, electrical, and thermal properties. As nanofillers have a high surface area, the interaction between the nanofiller and the polymer matrix is an essential issue for these materials. The main aim of this study is to validate the impact of the montmorillonite nanofiller...
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Pesticides in the Environment
PublicationApplied all over the world, pesticides are some of the most dangerous pollutants of the environment becauseof their mobility and ability to accumulate in the environment and their consequent long-term adverseeffects on living organisms in general and human health in particular. For these reasons, it is essential tomonitor and analyze pesticide residues in the environment. This entry discusses the classification of pesticidesas...
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Determination of organophosphorus and organonitrogen pesticides in water samples
PublicationPesticides are among the most dangerous environmental pollutants because of their stability, mobility and long-term effects on living organisms. Their presence in sources of drinking water, essential to life, is a particular danger. In water, these compounds can undergo transformations, leading to the production of substances of even greater toxicity. Legal regulations are in force, stipulating the highest permissible level of...
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Diagonalized Macromodels in Finite Element Method for Fast Electromagnetic Analysis of Waveguide Components
PublicationA new technique of local model-order reduction (MOR) in 3-D finite element method (FEM) for frequency-domain electromagnetic analysis of waveguide components is proposed in this paper. It resolves the problem of increasing solution time of the reduced-order system assembled from macromodels created in the subdomains, into which an analyzed structure is partitioned. This problem becomes particularly relevant for growing size and...
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Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces
PublicationIn this work, we study the electrodes with a periodic matrix of gold particles pattered by titanium dimples and modified by 3-mercaptopropionic acid (MPA) followed by CD147 receptor grafting for specific impedimetric detection of SARS-CoV-2 viral spike proteins. The synergistic DFT and MM/MD modeling revealed that MPA adsorption geometries on the Au–Ti surface have preferential and stronger binding patterns through the carboxyl...
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Unveiling the Pool of Metallophores in Native Environments and Correlation with Their Potential Producers
PublicationFor many organisms, metallophores are essential biogenic ligands that ensure metal scavenging and acquisition from their environment. Their identification is challenging in highly organic matter rich environments like peatlands due to low solubilization and metal scarcity and high matrix complexity. In contrast to common approaches based on sample modification by spiking of metal isotope tags, we have developed a two-dimensional...
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Review on mechanisms and efficiency of removal of microbiological contaminants in constructed wetlands
PublicationConstructed wetlands (CW) have been considered as a waste and a stormwater treatment systems for small communities or for areas with unsteady sewage flow conditions. Several investigations were undertaken for estimation suspended solids, organic matter and nutrients efficiency removal but only few focused on retention of microorganisms in constructed wetlands. In this review mechanisms of elimination of viruses, indicator bacteria...
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Fuzyjne polimerazy DNA – otrzymywanie, charakterystyka i zastosowanie
PublicationObecnie reakcje PCR (ang. Polymerase Chain Reaction) wykazują bardzo szerokie zastosowanie w diagnostyce medycznej, biologii molekularnej czy inżynierii genetycznej. Efektywność tych reakcji rozumiana jako wydajność i wierność przeprowadzonej amplifikacji jest nieodłącznie związana ze stosowaną polimerazą DNA i warunkami prowadzenia reakcji PCR. Aby sprostać wymaganiom stawianym przez nowoczesne metody diagnostyczne oraz współczesną...
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More than just a beer – Brewers' spent grain, spent hops, and spent yeast as potential functional fillers for polymer composites
PublicationBeer is among the most popular beverages in the world, with the production distributed uniformly between the biggest continents, so the utilization of brewing by-products is essential on a global scale. Among their potential recipients, the plastics industry offers extensive range of potential products. Herein, the presented study investigated the application of currently underutilized solid brewing by-products (brewers' spent...
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Modelling and analysis of medium frequency transformers for power converters
PublicationThe evolutions in power systems and electric vehicles, related to the economic opportunities and the environmental issues, bring the need of high power galvanically isolated DC-DC converter. The medium frequency transformer (MFT) is one of its key components, enabled by the increasing switching frequency of modern power semiconductors like silicon carbide transistors or diodes. The increased operating frequency offers small...
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Production of essential oils from in vitro cultures of Caryopteris species and comparison of their concentrations with in vivo plants
PublicationThe chemical composition of hydrodistilled essential oils obtained from aerial parts and roots of selected Caryopteris (‘bluebeard’) species (C. incana, C. mongolica, Caryopteris 9 clandonensis), as well as the newly established in vitro shoot and adventitious root cultures of the above plants, was analyzed by gas chromatography– mass spectrometry. Essential oil content and composition differed significantly depending on the type...
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FROM HARVESTING TO DISTILLATION - EFFECT OF ANALYTICAL PROCEDURES ON THE YIELD AND CHEMICAL COMPOSITION OF RHODODENDRON TOMENTOSUM (LEDUM PALUSTRE) ESSENTIAL OIL
PublicationRhododendron tomentosum possesses the anti-inflammatory, analgesic and antimicrobial properties, determined by the chemical composition of its essential oil. The effects of place (Miszewko, Lubichowo) and time of harvesting (June, November) as well as drying (air-drying, oven-drying, freeze-drying) and isolation (in Deryng, Clevenger and Likens-Nickerson apparatus) procedures on the yield and quality of R. tomentosum essential...
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Essential Oils, Silver Nanoparticles and Propolis as Alternative Agents Against Fluconazole Resistant Candida albicans, Candida glabrata and Candida krusei Clinical Isolates
PublicationDevelopment of effective and safe therapeutic treatment of fungal infections remains one of the major challenge for modern medicine. The aim of presented investigation was to analyze the in vitro antifungal activity of selected essential oils, ethanolic extracts of propolis and silver nanoparticles dropped on TiO2 against azole-resistant C. albicans (n = 20), C. glabrata (n = 14) and C. krusei (n = 10) clinical isolates. Among...
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Advancements in Diamond-Like Carbon Coatings
PublicationAn essential resource for engineers and scientists in the coatings field, providing an in-depth examination of current and advanced technologies for industrially oriented nanoceramic and nanocomposite coatings
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Fluctuation-enhanced scent sensing using a single gas sensor
PublicationScent or aroma sensing during aromatherapy can be carried out by applying only a single resistance gas sensor (TGS - Taguchi Gas Sensors). This paper considers the efficiency of detection of essential oils by DC resistance and its fluctuations observed in TGS sensors. A detailed study has been conducted for scents emitted by five popular essential oils using three sensor types (TGS 2600, TGS 2602, TGS 823). The research was focused...
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Defining a novel domain that provides an essential contribution to site-specific interaction of Rep protein with DNA
PublicationAn essential feature of replication initiation proteins is their ability to bind to DNA. In this work, we describe a new domain that contributes to a replication initiator sequence-specific interaction with DNA. Applying biochemical assays and structure prediction methods coupled with DNA–protein crosslinking, mass spectrometry, and construction and analysis of mutant proteins, we identified that the replication initiator of the...
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Experimental determination of dynamic properties of cylindrical steel tank model filled with liquid
PublicationIn technical branches, such as chemical or petroleum industries, cylindrical steel tanks are essential structures used for storage of liquid products. Any failure might have disastrous consequences, therefore their safety and reliability is essential. The aim of the present paper is to show the results of the experimental study which has been conducted on a scaled model of a real tank used in Poland. The investigation was carried...
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Spatially variant PSF modeling in confocal macroscopy
PublicationThe point spread function (PSF) of imaging systems plays an essential role in image reconstruction. In the context of confocal microscopy, optical performance degrades towards the edge of the field of view. In confocal macroscopy, the related artifacts are even stronger, as the field of view is much larger. Because the related PSFs are strongly spatially variant, it is essential to be able to model them with few parameters. The...
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Investigation on air quality of specific indoor environments—spa salons located in Gdynia, Poland
PublicationDue to excessive application of essential oils and scented products in spa salons during aromatherapy and massage sessions, the elevated concentration of total volatile organic compounds (TVOCs), particularly terpenes, which are known as secondary organic aerosol (SOA) precursors, is expected there. This study was aimed at determination of VOCs with a particular regard to terpenes in air samples collected in selected spa salons...
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Lipopolysaccharides: regulated biosynthesis and structural diversity
PublicationThe cell envelope of Gram-negative bacteria contains two distinct membranes, an inner (IM) and an outer (OM) membrane, separated by the periplasm, a hydrophilic compartment that includes a thin layer of peptidoglycan. The most distinguishing feature of such bacteria is the presence of an asymmetric OM with phospholipids located in the inner leaflet and lipopolysaccharides (LPSs) facing the outer leaflet. The maintenance of this...
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Regulation of the First Committed Step in Lipopolysaccharide Biosynthesis Catalyzed by LpxC Requires the Essential Protein LapC (YejM) and HslVU Protease
PublicationWe previously showed that lipopolysaccharide (LPS) assembly requires the essential LapB protein to regulate FtsH-mediated proteolysis of LpxC protein that catalyzes the first committed step in the LPS synthesis. To further understand the essential function of LapB and its role in LpxC turnover, multicopy suppressors of ΔlapB revealed that overproduction of HslV protease subunit prevents its lethality by proteolytic degradation...
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The influence of Nigella sativa essential oil on proliferation, activation, and apoptosis of human T lymphocytes in vitro
PublicationIn previous work, we tested the immunomodulatory effect of Nigella sativa (NS) fatty oil. Our results demonstrated that unrefined, obtained by cold pressing black cumin seed oil inhibited lymphocytes’ proliferation and induced their apoptosis in a dose-dependent manner. In this study, we examined the immunomodulatory properties of essential oil (EO) obtained from the NS seeds by hydrodistillation and its two main constituents:...
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Response of a steel tank during earthquakes and mining tremors - experimental investigation
PublicationCylindrical steel tanks are very popular shell structures used to store the products of chemical and petroleum industries. Any failure might have disastrous consequences, therefore their safety and reliability is essential. Earthquakes are the most dangerous and also the most unpredictable dynamic loads acting on such structures. On the other hand, mining tremors are usually considered to be less severe due to lower acceleration...
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A Survey Investigating the Influence of Business Analysis Techniques on Software Quality Characteristics
PublicationBusiness analysis is recognized as one of the most important areas determining the outcome (success or failure) of a software project. In this paper we explore this subject further by investigating the potential impact of techniques applied in business analysis on essential software quality characteristics. We conducted a literature search for software quality models, analyzed the existing models and selected a subset of commonly...
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A new factor LapD is required for the regulation of LpxC amounts and lipopolysaccharide trafficking
PublicationLipopolysaccharide (LPS) constitutes the major component of the outer membrane and is essential for bacteria, such as Escherichia coli. Recent work has revealed the essential roles of LapB and LapC proteins in regulating LPS amounts; although, if any additional partners are involved is unknown. Examination of proteins co-purifying with LapB identified LapD as a new partner. The purification of LapD reveals that it forms a complex...
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Evaluation of the yield, chemical composition and biological properties of essential oil from bioreactor-grown cultures of Salvia apiana microshoots
PublicationMicroshoot cultures of the North American endemic Salvia apiana were established for the first time and evaluated for essential oil production. Stationary cultures, grown on Schenk-Hildebrandt (SH) medium, supplemented with 0.22 mg/L thidiazuron (TDZ), 2.0 mg/L 6-benzylaminopurine and 3.0% (w/v) sucrose, accumulated 1.27% (v/m dry weight) essential oil, consisting mostly of 1,8-cineole, β-pinene, α-pinene, β-myrcene and camphor....
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Checkpoints that regulate balanced biosynthesis of lipopolysaccharide and its essentiality in Escherichia coli
PublicationThe outer membrane (OM) of Gram-negative bacteria, such as Escherichia coli, is essential for their viability. Lipopolysaccharide (LPS) constitutes the major component of OM, providing the permeability barrier, and a tight balance exists between LPS and phospholipids amounts as both of these essential components use a common metabolic precursor. Hence, checkpoints are in place, right from the regulation of the first committed step...
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Optical sensor of a person sitting on a chair and dressed in multi-layered clothes vital signs monitoring
PublicationAn optoelectronic device enhancing a smart chair functionality is presented in the paper. Its essential purpose is a detection of a sitting person presence on the chair by means of detecting the vital signs. Additionally, it could be used for determining of clothes layer parameters useful in adjusting a system of a capacitive electrocardiography.
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Hunting for the Magnesium(I) Species: Formation, Structure, andReactivity of some Donor-Free Grignard Compounds
PublicationMagnesium bromide radicals have to be prepared as high-temperature molecules and trapped as a metastable solution because a seemingly simple reduction of donor-free Grignard compounds failed. However, the essential role of magnesium(I) species during the formation of Grignard compounds could be demonstrated experimentally.
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Inhibitors of amino acids biosynthesis as antifungal agents
PublicationFungal microorganisms, including the human pathogenic yeast and filamentous fungi, are able to synthesize all proteinogenic amino acids, including nine that are essential for humans. A number of enzymes catalyzing particular steps of human-essential amino acid biosynthesis are fungi specific. Numerous studies have shown that auxotrophic mutants of human pathogenic fungi impaired in biosynthesis of particular amino acids exhibit...
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Bee Products and Essential Oils as Alternative Agents for Treatment of Infections Caused by S. aureus
PublicationBacteria of the genus Staphylococcus are important human and veterinary pathogens. A crucial characteristic for this group of bacteria is that they can easily acquire mechanisms of antibiotic resistance for a plethora of antibiotics currently in use for human and animal therapies. Therefore, there is a great need to find novel, non-antibiotic chemotherapeutics with marked antistaphylococcal activity. Promising but still underestimated...
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Regulation of LPS assembly via controlled proteolysis and sensing of LPS stress in Escherichia coli
PublicationLipopolysaccharide (LPS) is a complex glycolipid, essential for the bacterial viability and along with phospholipids, it constitutes the major amphiphilic component of outer membrane (OM) in most of the Gram-negative bacteria, including Escherichia coli. LPS molecules confer an effective permeability barrier to the OM and play a crucial role in bacteria-environment and -host interactions. The synthesis and accumulation of this...
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Validated HPTLC method for determination of ledol and alloaromadendrene in the essential oil fractions of Rhododendron tomentosum plants and in vitro cultures and bioautography for their activity screening
PublicationRhododendron tomentosum (Ledum palustre) is a bog shrub used in traditional medicine for treatment of respiratory and rheumatic diseases. Due to the large variability of the chemical composition of its essential oil, depending on the habitat, the in vitro cultures were established as the alternative source of the volatile fraction. There is a need to monitor a quality of the field grown as well as in vitro plant material, especially...
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Specificity of Infant Digestive Conditions: Some Clues for Developing Relevant In Vitro Models
PublicationDigestion of nutrients is an essential function of the newborn infant gut to allow growth and development and understanding infant digestive function is essential to optimize nutrition and oral drug delivery. Ethical considerations prohibit invasive in vivo trials and as a consequence in vitro assays are often conducted. However, the choice of in vitro model parameters are not supported by an exhaustive analysis of the literature...
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DETERMINATION OF THE HYDROXYL NUMBER OF GROUND TIRE RUBBER PARTICLES VIA MODIFIED TEST METHOD FOR ISOCYANATE GROUPS
PublicationNowadays, considering the environmental trends and law regulations associated with the circular economy, it is very important to seek for the methods of by-products and waste utilization. The example of such material, which requires the attention and recycling method is ground tire rubber generated during recycling of post-consumer car tires. It can be introduced into various polymer matrices as a filler, but to enhance its effectivity...
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Usage of Memi-Markov Process in Operation Evaluation of Diesel Engine
PublicationIn paper, the proposition of quantitative evaluation of operation using semi-Markov processes theories has been presented. Basic assumptions, essential for creating mathematical model on example of diesel engine were shown. Special attention was given to practical aspects of using established mathematical model. Example of characteristic of analysed process - temporary distribution of probability was assigned.
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New technologies using Trace Metals of Concern
PublicationThis chapter discusses the occurrence, material flows, technical applications of and pollution by platinum, palladium, thallium, rare earth elements, gold , silver, and antimony, all of which are essential for global economic growth. Modern technology relies on theses chemicals for the production of various industrial goods. however, their use releases pollutants into the environment, thus posing a threat to human health.
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Generation of random fields to reflect material and geometric imperfections of plates and shells
PublicationThe paper covers two patterns of random field generation: conditional acceptance – rejection method and Karhunen – Loève expansion. The generation of two-dimensional random fields is essential in plates and shells analysis, allowing for a relevant limit and critical state assessment of geometrically and ma-terially imperfect structures. The features of both generation methods dedicate them to selected problems.
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PHYSICAL MODELLING OF GEOTECHNICAL STRUCTURES IN PORTS AND OFFSHORE
PublicationThe physical modelling of subsoil behaviour and soil-structure interaction is essential for the proper design of offshore structures and port infrastructure. A brief introduction to such modelling of geoengineering problems is presented and some methods and experimental devices are described. The relationships between modelling scales are given. Some examples of penetration testing results in centrifuge and calibration chamber...