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Cloning, expression, and purification of a recombinant cold-adapted β-galactosidase from antarctic bacterium Pseudoalteromonas sp. 22b
PublicationAntarktyczny szczep, gram-ujemnych bakterii Pseudoalteromonas sp. 22b, wyizolowanych z przewodu pokarmowego kryla z gatunku Thyssanoessa macrura, jest producentem wewnątrzkomórkowej zimnolubnej beta-galaktozydazy. Białko to jest homotetramerem, w którym każda z jednostek monomeru zbudowana jest z 1028 reszt aminokwasowych. Gen kodujący ten enzym został sklonowany i eksprymowany w komórkach E. coli. Następnie z zastosowaniem procedury...
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Glukuronidacja leków przeciwnowotworowych – detoksyfikacja, mechanizm oporności czy sposób na formę proleku?*
PublicationKsenobiotyki podlegają w organizmie biotransformacji I i II fazy, której celem jest ich detoksyfikacja i ułatwienie wydalania. UDP-glukuronylotransferazy (UGT) – enzymy należące do II fazy metabolizmu katalizują przyłączenie kwasu glukuronowego do lipofilowego substratu zawierającego nukleofilową grupę funkcyjną. Nadrodzina UGT składa się z izoenzymów o różnej swoistości substratowej oraz odmiennym profilu ekspresji w poszczególnych tkankach....
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Genotyping and characterization of virulence factors in Escherichia coli strains isolated from patients from Polish hospital
PublicationZakażenia układu moczowego (UTIs - ang. urinary track infections) są jedną z najczęstszy infekcji, za które w 85% przypadków odpowiedzialne są bakterie Escherichia coli. Infekcje układu moczowego mogą prowadzić do groźnych powikłań m. in. odmiedniczkowego zapalenia nerek. Śmiertelność wśród tej grupy pacjentów wynosi 1-3%. Zakażenia układu moczowego występują również u ok. 7% kobiet ciężarnych. W pracy przebadano 82 szczepy E....
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Hydrophobic derivatives of 2-amino-2-deoxy-D-glucitol-6-phosphate: a newtype of D-glucosamine-6-phosphate synthase inhibitors with antifungal action.
PublicationDokonano syntezy szeregu N-acylowych i estrowych pochodnych 2-amino-2-deok-sy-glucitolo-6-fosforanu (ADGP) i zbadano ich aktywność przeciwgrzybową oraz wobec enzymów zaangażowanych we wczesne etapy biosyntezy chityny w komórkach grzybów. Wszystkie badane pochodne okazały się słabszymi inhibitorami enzymu syntezy GlcN-6-P aniżeli ADGP, ale niektóre z nich wykazywały znacznie wyższą aktywność przeciwgrzybową niż związek macierzysty....
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Scheduling of identical jobs with bipartite incompatibility graphs on uniform machines. Computational experiments
PublicationWe consider the problem of scheduling unit-length jobs on three or four uniform parallel machines to minimize the schedule length or total completion time. We assume that the jobs are subject to some types of mutual exclusion constraints, modeled by a bipartite graph of a bounded degree. The edges of the graph correspond to the pairs of jobs that cannot be processed on the same machine. Although the problem is generally NP-hard,...
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CMOS implementation of an analogue median filter for image processing in real time
PublicationAn analogue median filter, realised in a 0.35 μm CMOS technology, is presented in this paper. The key advantages of the filter are: high speed of image processing (50 frames per second), low-power operation (below 1.25 mW under 3.3 V supply) and relatively high accuracy of signal processing. The presented filter is a part of an integrated circuit for image processing (a vision chip), containing: a photo-sensor matrix, a set of...
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Inhibitors of glucosamine-6-phosphate synthase as potential antimicrobials or antidiabetics – synthesis and properties
PublicationGlucosamine-6-phosphate synthase (GlcN-6-P synthase) is known as a promising target for antimicrobial agents and antidiabetics. Several compounds of natural or synthetic origin have been identified as inhibitors of this enzyme. This set comprises highly selective L-glutamine, amino sugar phosphate or transition state intermediate cis-enolamine analogues. Relatively low antimicrobial activity of these inhibitors, poorly penetrating...
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Fusion of DNA-binding domain of Pyrococcus furiosus ligase with TaqStoffel DNA polymerase as a useful tool in PCR with difficult targets
PublicationThe DNA coding sequence of TaqStoffel polymer- ase was fused with the DNA-binding domain of Pyrococcus furiosus ligase. The resulting novel recombinant gene was cloned and expressed in E. coli. The recombinant enzyme was purified and its enzymatic features were studied. The fusion protein (PfuDBDlig-TaqS) was found to have enhanced processivity as a result of the conversion of the Taq DNA polymerase from a relatively low processive...
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Systemy ekspresyjne białek cytochromu P450 w badaniach in vitro metabolizmu leków = Expression systems of cytochrome P450 proteins in studies of drug metabolism in vitro
PublicationBiałka cytochromu P450 to najważniejsze enzymy biorące udział w metabolizmie większości stosowanych w klinice leków, odpowiedzialne za ich aktywację lub detoksykację. Niektóre z dróg metabolizmu leku mogą być jednak odpowiedzialne za jego podwyższoną toksyczność. Nowe systemy ekspresyjne białek cytochromu P450 w komórkach ssaków, w tym człowieka, projektowane są w celu poznania roli metabolizmu w mechanizmie działania potencjalnych,...
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Analiza in silico bioaktywnych peptydów pochodzących z keratyny z piór drobiowych
PublicationKeratyna należy do najbardziej powszechnie występujących białek strukturalnych pochodzenia zwierzęcego. Stanowi ok. 90% masy piór wytwarzanych w dużych ilościach przez przemysł drobiarski. Ten odpadowy produkt pozostaje w dużym stopniu niezagospodarowany. Celem pracy była analiza bioinformatyczna pierwszorzędowej struktury keratyny z piór kurzych pod kątem występowania w niej peptydów bioaktywnych....
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Microcapsules and their applications in pharmaceutical and food industry.
PublicationMicroencapsulation is a process in which tiny particles or droplets are surrounded by a coating or embedded in a homogeneous or heterogeneous matrix, to generate small capsules in size range from a few to few thousand micrometers. The material inside the microcapsule is referred to as the core, internal phase, or fill, whereas the wall is sometimes called shell, coating, wall material, or membrane. It has found application in...
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Unieruchamianie rekombinowanej beta-galaktozydazy w reakcjach katalizowanych transglutaminazą
PublicationTermostabilną beta-galaktozydazę wyizolowaną z Escherichia coli zawierającej gen enzymu z Pyrococcus woesei, sieciowano transgluta-minazą na żelu krzemionkowym. Otrzymany preparat wykazuje w 70 st.C aktywność hydrolizy ONPG wynoszącą 11,573 U/g nośnika. Optymalne pH i temperatura działania enzymu wynosi odpowiednio 5,5 oraz 95 st.C. Preparat unieruchomionego enzymu zachowuje około 70 % początkowej aktywności po 1 godzinie inkubacji...
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Effects of Basalt and Carbon Fillers on Fire Hazard, Thermal, and Mechanical Properties of EPDM Rubber Composites
PublicationDue to growing restrictions on the use of halogenated flame retardant compounds, there is great research interest in the development of fillers that do not emit toxic compounds during thermal decomposition. Polymeric composite materials with reduced flammability are increasingly in demand. Here, we demonstrate that unmodified graphene and carbon nanotubes as well as basalt fibers or flakes can act as effective flame retardants...
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Photolysis for the Removal and Transformation of Pesticide Residues During Food Processing: A State-of-the-Art Minireview
PublicationPesticide residues are of great significant issue that exerted adverse effects on humans. There is a need for effective and non-toxic decontamination of pesticide residues during food processing. In this minireview, the recent advances in the degradation of pesticide residues by photolysis have been firstly described during food processing. The mechanisms of pesticide residues destruction by photolysis were discussed accordingly....
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THE POSSIBILITIES OF ESTIMATING THE RELIABILITY OF SHIP PIPELINES’ ELEMENTS INCLUDING DESTRUCTIVE PHENOMENA ACTING ON THEM
PublicationIn the article an approach to the problem of estimating reliability data based on physical models is proposed. The possibility of reliability assessment for selected elements of ship pipelines, based on the recognition of the destructive physical phenomena taking place in them, is discussed. To do this, an overview of these phenomena has been made. In addition, a preliminary review of existing measures of destruction of materials...
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Field-induced suppression of charge density wave in GdNiC2
PublicationWe report the specific heat, magnetic, magnetotransport, and galvanomagnetic properties of polycrystalline GdNiC 2 . In the intermediate temperature region above T N = 20 K, we observe large negative magnetoresistance due to Zeeman splitting of the electronic bands and partial destruction of a charge density wave ground state. Our magnetoresistance and Hall measurements show that at low temperatures a magnetic field-induced transformation from...
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The role of glucuronidation in drug resistance
PublicationThe final therapeutic effect of a drug candidate, which is directed to a specific molecular target strongly depends on its absorption, distribution, metabolism and excretion (ADME). The disruption of at least one element of ADME may result in serious drug resistance. In this work we described the role of one element of this resistance: phase II metabolism with UDP-glucuronosyltransferases (UGTs). UGT function is the transformation...
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Bee Pollen and Bee Bread as a Source of Bacteria Producing Antimicrobials
PublicationThe principal objective of the study was the isolation and identification of bacteria that are present in mature bee bread (BB) and dried (ready for selling and consumption) bee pollen (BP). Obtained isolates were screened for their potential to inhibit select human pathogenic bacteria and their ability to produce enzymes of particular industrial importance. Four and five samples of BP and BB, respectively, were used for the study....
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Impact of high-pressure processing on the bioactive compounds of milk - A comprehensive review
PublicationHigh-pressure processing (HPP) is a promising alternative to thermal pasteurization. Recent studies highlighted the effectivity of HPP (400–600 MPa and exposure times of 1–5 min) in reducing pathogenic microflora for up to 5 logs. Analysis of modern scientific sources has shown that pressure affects the main components of milk including fat globules, lactose, casein micelles. The behavior of whey proteins under HPP is very important...
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Purification and biochemical characteristic of a cold-active recombinant esterase from Pseudoalteromonas sp. 643A under denaturing conditions.
PublicationIn this paper production of a cold-active esterase EstA from the Antarctic bacterium Pseudoalteromonas sp. 643A in E. coli expression system was described. The purification and biochemical characteristic of EstA were performed in the presence of urea and then compared with results obtained for the esterase with no addition of urea and isolated from the native source. In both cases the cold-active enzyme displayed similar properties....
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Beta-D-galaktozydazy - źródła, właściwości i zastosowanie
PublicationArtykuł przeglądowy opisujący mechanizm działania enzymu beta-D-galaktozydazy, właściwości beta-D-galaktozydaz pochodzących z różnych mikroorganizmów, zarówno mezofilnych jak i psychrofilnych oraz termofilnych, a także możliwości zastosowania enzymu w przemysłowych procesach hydrolizy laktozy w mleku, serwatce oraz w reakcji syntezy galaktooligosacharydów.
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Electrochemicalsynthesis of 3D nano-/micro-structured porous polypyrrole
PublicationIn this work, electrosynthesis of electroactive, 3D nano-/micro-structured porous polypyrrole film is presented. The PPy film was synthesized potentiostatically in a one-step process from aqueous solution of pyrrole and lithium perchlorate. The growth mechanism of such structure included: the formation of typical globular PPy film, followed by the formation of the PPy fibers, which then took part in the formation of 3D highly porous...
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Human carnosinases: A brief history, medicinal relevance, and in silico analyses
PublicationCarnosine, an endogenous dipeptide, has been found to have a plethora of medicinal properties, such as antioxidant, antiageing, and chelating effects, but with one downside: a short half-life. Carnosinases and two hydrolytic enzymes, which remain enigmatic, are responsible for these features. Hence, here we emphasize why research is valuable for better understanding crucial concepts like ageing, neurodegradation, and cancerogenesis,...
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INFLUENCE OF A VERTEX REMOVING ON THE CONNECTED DOMINATION NUMBER – APPLICATION TO AD-HOC WIRELESS NETWORKS
PublicationA minimum connected dominating set (MCDS) can be used as virtual backbone in ad-hoc wireless networks for efficient routing and broadcasting tasks. To find the MCDS is an NP- complete problem even in unit disk graphs. Many suboptimal algorithms are reported in the literature to find the MCDS using local information instead to use global network knowledge, achieving an important reduction in complexity. Since a wireless network...
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Speed management on local government managed roads – research, recommendations and guidelines
PublicationCommissioned by the National Road Safety Council Secretariat, the project “Guidelines for speed management on local government managed roads” studied car driver be haviour when subjected to selected speed management measures such a local speed restrictions, surveillance, traffic calming and restricted speed areas. In addition, analyses were conducted on the impact of selected me asures...
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Revitalization of the Narrow-Gauge Żuławy Commuter Railway and its effect on shipping conditions in the Vistula delta
PublicationThe article presents the Żuławy Access Railway and the effect of its revitalization on the change of navigational conditions in the Vistula delta. One of the problems analyzed in the article is the intersection of two different transport branches - the inland waterway and rail transport branches. A solution to the problem of their functioning is presented, without any major restrictions. Possible changes in navigational conditions...
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DNA adduct formation by C-1748, a potent antitumor 4-methyl-1-nitroacridine of lowered toxicity.
Publication4-podstawione 1-nitroakrydyny reprezentują nową grupę pochodnych zsyntetyzowanych na Politechnice Gdańskiej. W porównaniu do macierzystych 1-nitroakrydyn związki te wykazują obniżoną toksyczność i podwyższoną aktywność przeciwnowotworową. Wiodąca pochodna 4-metylo-1-nitroakrydyna o symbolu C-1748 jest obecnie w przygotowaniu do I fazy badań klinicznych w leczeniu raka okrężnicy. Wprowadzenie elektrodonorowej grupy metylowej w...
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Magnetism and charge density waves in RNiC2(R=Ce,Pr,Nd)
PublicationWe have compared the magnetic, transport, galvanomagnetic, and specific-heat properties of CeNiC2, PrNiC2, and NdNiC2 to study the interplay between charge density waves (CDW) and magnetism in these compounds. The negative magnetoresistance in NdNiC2 is discussed in terms of the partial destruction of charge density waves and an irreversible phase transition stabilized by the field-induced ferromagnetic transformation is reported....
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Explicit and implicit difefrence methods for quasilinear first order partial functional differential equations.
PublicationInitial boundary value problems of the Dirichlet type for quasilinear functional differential equations are considered. Explicit difference schemes of the Euler type and implicit difference methods are investigated. Suffcient conditions for the convergence of approximate solutions are given and comparisons of the methods are presented. It is proved that assumptions on the regularity of given functions are the same for both classes...
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On well-posedness of the first boundary-value problem within linear isotropic Toupin–Mindlin strain gradient elasticity and constraints for elastic moduli
PublicationWithin the linear Toupin–Mindlin strain gradient elasticity we discuss the well-posedness of the first boundary-value problem, that is, a boundary-value problem with Dirichlet-type boundary conditions on the whole boundary. For an isotropic material we formulate the necessary and sufficient conditions which guarantee existence and uniqueness of a weak solution. These conditions include strong ellipticity written in terms of higher-order...
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Testing of Technical Fabrics under Fast Camera Control
PublicationThe dynamic development of measurement and recording techniques has been changing the way one conceives material strength. In this study, two different methods of evaluating the strength of fabrics are compared. The first is the typical and commonly used technique based on the use of a testing machine. The second method uses the so-called “fast camera” to monitor the entire process of the destruction of a fabric sample and analyse...
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Młode Miasto Gdańsk (1380-1455) i jego patrymonium
PublicationW monografii ustalono m.in.: 1) położenie strefy osadniczej Młodego Miasta i zasięg jego patrymonium, 2) liczbę i (w miarę możliwości) lokalizację obiektów sakralnych w tym ośrodku, 3) zmiany w statusie parafialnym kościoła św Bartłomieja, 4) przemiany demograficzne ośrodka młodomiejskiego, 5) związki elity politycznej Młodego Miasta z Głównym Miastem oraz ich kontakty z Zakonem, 6) rolę portu Młodego Miasta jako ośrodka pomocniczego...
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Numerical and experimental study on effect of boundary conditions during testing of stiffened plates subjected to compressive loads
PublicationThis study analyses the effect of boundary conditions during testing on the structural behaviour stiffened plates with different thicknesses subjected to compressive loads. The goal of the compressive tests is to analyse the ultimate strength of a stiffened plate. During the test, relevant physical quantities are measured and investigated. The supporting structure's behaviour is investigated by analysing the force-displacements...
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Long range molecular dynamics study of regulation of eukaryotic glucosamine-6-phosphate synthase activity by UDP-GlcNAc
PublicationGlucosamine-6-phosphate (GlcN-6-P) synthase catalyses the first and practically irreversible step in hexosamine metabolism. The final product of this pathway, uridine 5' diphospho N-acetyl-D-glucosamine (UDPGlcNAc), is an essential substrate for assembly of bacterialand fungal cell walls. Moreover, the enzyme is involved in phenomenon of hexosamine induced insulin resistance in type II diabetes, which makes it a potential target...
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Recombinant !ermostable AP Exonuclease from Thermoanaerobacter tengcongensis: Cloning, Expression, Purification, Properties and PCR Application
PublicationApurinic/apyrimidinic (AP) sites in DNA are considered to be highly mutagenic and must be corrected to preserve genetic integrity, especially at high temperatures. !e gene encoding a homologue of AP exonuclease was cloned from the thermophilic anaerobic bacterium Thermoanaerobacter tengcongensis and transformed into Escherichia coli. The protein product showed high identity (80%) to human Ape1 nuclease, whereas to E. coli exonuclease...
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Applying agiile practices to the development of safety-critical software
PublicationDevelopment of safety critical software is subjected to numerous restrictions and recommendations. To meet these requirements classical software development models (e.g. V-model) are usually applied. Agile methodologies, although rapidly increasing their popularity, were generally regarded as unsuitable for such projects. However, they offer potential benefits which provide a strong incentive to incorporate them into safety-critical...
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Evaluation of Vehicle Routing Problem Algorithms for Transport Logistics Using Dedicated GIS System
PublicationThe development and research related to optimization of fleet management is of high interest among many industrial and scientific entities related to logistics and transport. Optimal distribution of transportation resources leads to significant cost reduction. In this context, scientific research related to so called Vehicle Routing Problem (VRP) which relies on determining the shortest transport routes for a strictly limited number...
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Novel 1,2,3-Triazole Derivatives as Mimics of Steroidal System—Synthesis, Crystal Structures Determination, Hirshfeld Surfaces Analysis and Molecular Docking
PublicationHerein, we present the synthesis and crystal structures determination of five 4-(1-phenyl-1H-1,2,3-triazol-4-yl)phenol derivatives containing halogen atoms, 6a–e, which may be used as an excellent mimic of steroids in the drug development process. Good quality crystals obtained for all of the synthesized compounds allowed the analysis of their molecular structures. Subsequently, the determined crystal structures were used to calculate...
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Novel insights into conjugation of antitumor-active unsymmetrical bisacridine C-2028 with glutathione: characteristics of non-enzymatic and glutathione S-transferase-mediated reactions
PublicationUnsymmetrical bisacridines (UAs) are a novel potent class of antitumor-active therapeutics. A significant route of phase II drug metabolism is conjugation with glutathione (GSH), which can be non-enzymatic and/or catalyzed by GSH-dependent enzymes. The aim of this work was to investigate the GSH-mediated metabolic pathway of a representative UA, C 2028. GSH supplemented incubations of C-2028 with rat, but not with human, liver...
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Investigation of interaction between xanthene dye rhodamine B and metagenomic-derivated methylthioadenosine phosphorylase RSFP
PublicationIn the previous study we found that the expression of the metagenomic - derived methylthioadenosine phosphorylase gene (rsfp) in E. coli cells and the presence of rhodamine B in the growth medium were crucial for the pink fluorescence of one E. coli colony from Antarctic soil metagenomic library (Cieśliński et al., 2009). In this study, rsfp gene expression was induced by an arabinose promoter in an LMG194 E. coli strain and the...
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Immobilized preparation of cold-adapted and halotolerant Antarctic β-galactosidase as a highly stable catalyst in lactose hydrolysis
PublicationZimnolubna rekombinowana beta-galaktozydaza antarktycznej morskiej bakterii Pseudoalteromonas sp. 22b została immobilizowana na chitozanie za pomocą aldehydu glutarowego. Preparat immobilizowanego enzymu w przeciwieństwie do preparatu enzymu w buforze reakcyjnym nie był inhibowany przez glukozę. Immobilizacja enzymu wpłynęła na podwyższenie o 10°C optymalnej temperatury aktywności względem jego nieimmobilizowanego odpowiednika...
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Linear Pantographic Sheets: Existence and Uniqueness of Weak Solutions
Publicationwe address the well-posedness of the planar linearized equilibrium problem for homogenized pantographic lattices. To do so: (i) we introduce a class of subsets of anisotropic Sobolev’s space as the most suitable energy space E relative to assigned boundary conditions; (ii) we prove that the considered strain energy density is coercive and positive definite in E ; (iii) we prove that the set of placements for which the strain...
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EXPERIMENTAL EVALUATION OF DLC/STEEL SLIDING CONTACTS’ OPERATIONAL ENVELOPE
PublicationIn an industrial project concerning sliding bearings, a sliding pair was selected of high alloy steel vs. industrial grade DLC coated high alloy steel as a basis for an innovative design of high performance sliding bearings lubricated with a synthetic oil for use in geared transmission units. The development process required credible data on the ultimate resistance of the sliding pair to very high contact stress. An experimental...
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Experimental research on marine oil-lubricated stern tube bearing
PublicationBearings of propeller shafts are very crucial elements of the propulsion system of each of the ships. The safety of shipping depends on their durability and reliability. The new legal restrictions mean that today we are looking for environmentally friendly solutions. That is why water-lubricated bearings are becoming more and more popular. So, will oil-lubricated shaft bearings belong to the past? The bearing with a white metal...
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Researching Digital Society: Using Data-Mining to Identify Relevant Themes from an Open Access Journal
PublicationOpen Access scholarly literature is scientific output free from economic barriers and copyright restrictions. Using a case study approach, data mining methods and qualitative analysis, the scholarly output and the meta-data of the Open Access eJournal of e-Democracy and Open Government during the time interval 2009–2020 was analysed. Our study was able to identify the most prominent research topics (defined as thematic clusters)...
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Polish tax reform "Polski Ład" – consequences of introducing changes on the example of selected flat-rate tax forms for micro and small companies
PublicationThis article describes aspects related to the introduction of the latest tax reform in Poland. The changes initiated in January 2022, under the name Polski Ład, concern a wide range of tax changes in Poland. Reform, in a broad sense, is a multidimensional government development program, which is primarily aimed at getting out of the crisis caused by the COVID-19 pandemic and a smooth transition to financing strategic goals in investment...
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Scaled agile framework. Dealing with software process‐related challenges of a financial group with the action research approach
PublicationThis article reports on a domain-specific software development venture at Nordea. We explore organizational constraints, challenges, and corrective actions undertaken when scaling the agile development approach of their Core Banking Platform program. The fit and required customizations of the rather complex and rigid SAFe framework in a policy-heavy financial institution are audited against the organic growth of the program. In...
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Researching Digital Society: Using Data-Mining to Identify Relevant Themes from an Open Access Journal
PublicationOpen Access scholarly literature is scientific output free from economic barriers and copyright restrictions. Using a case study approach, data mining methods and qualitative analysis, the scholarly output and the meta-data of the Open Access eJournal of e-Democracy and Open Government during the time interval 2009–2020 was analysed. Our study was able to identify the most prominent research topics (defined as thematic clusters)...
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ISSUES OF ECONOMIC ANALYSIS OF ELECTRIC ENERGY GENERATION IN A FLOATING POWER PLANT
PublicationThis article presents basic dependences for conducting a technical and economic analysis related with investment projects. It describes a combined system of a compression ignition engine and a steam turbine, whose aim, when placed on a floating platform, is to generate electric energy in the so-called distributed generation system. Such a system involves a reciprocating internal combustion engine and a connected steam turbine system...
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The potential of Web-GIS and geovisual analytics in the context of marine cadastre
PublicationThe importance of the marine environment to human existence makes it imperative that information models represent the multidimensional nature of reality as closely as possible in order to facilitate good governance. Information regarding jurisdiction, as well as the effects of formal law and community interests on the marine environment (e.g. nature and spatial extents as well as rights, responsibilities, restrictions etc.) would...