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Search results for: LACTIC ACID BACTERIA
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BACTERIAL INACTIVATION VIA LASER-DRIVEN GOLD NANOPARTICLE HEATING: SIMULATION AND ANALYSIS
PublicationThis study utilizes CFD technique to simulate the inactivation of E. coli bacteria within a microfluidic chamber, employing gold nanoparticles irradiated by a laser beam. Employing a single-phase model, the presence of bacteria is considered by treating thermal properties in the governing equations as effective, combining those of water and bacteria using established correlations from scientific literature. The conversion of light...
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Jan Hupka prof. dr hab. inż.
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INVESTIGATION OF ACIDIC SOLUTIONS BY POTENTIOMETRIC TASTE SENSOR WITH ALL SOLID STATE ELECTRODES
PublicationPotentiometric taste sensors with electrodes containing lipophilic compound / polymer membranes were already used to distinguish commercial liquid products. In this paper a new potentiometric taste sensor consisting of six All Solid State Electrodes (ASSEs) has been presented. Each of the membrane contains a selected lipophilic compound. The set-up consists of a reference electrode and six ASSEs. The inner layer made of a conducting...
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Stability Investigation of the PCM Nanocomposites
PublicationEnsuring the stability is a key issue to be solved for the technical application of nanocomposites. In this work, fatty acid P1801 served as base phase change material (PCM)P1801, and its main ingredients are palmitic acid (58%) and stearic acid (38%). Titania (TiO2) and alumina (Al2O3) with mass concentrations of 1% and 5% were selected as nanoparticles, while polyvinylpyrrolidone (PVP) or oleic acid (OA) with mass concentrations...
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Mechanism of Isomerization and Acid-Induced Transformations of 3-Hydroxy-4,4'-dimethoxy-3'-methyl-3-oxo-7,7'-bis(piperidino-carbonyloxy)-2,2'-spirobi-[2H,2'H,3H,3'H-benzo[b]thiophene]. Unusual Equilibrium between the Spiro and 3-Hydroxy-benzothiophene Systems.
PublicationStwierdzono, że 3'-hydroksy-4,4'-dimetoksy-3'-metylo-3-okso-7,7'-bis(piperydynokarbonyloksy)-2,2'-spirobi[2H,2'H,3H,3'H-benzo[b]tiofen] występuje w roztworach postaci mieszaniny dwóch diastereoizomerów pozostających w dynamicznej równowadze. Mechanizm tej równowagi oparty jest na odwracalnym otwarciu pierścienia z 1,5 przeniesieniem protonu i utworzeniu przjściowej formy 3-hydroksybenzotiofenu.
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The influence of moderate pressure and subzero temperature on the shelf life of minced cod, salmon, pork and beef meat
PublicationThe effect of moderate pressure at subzero temperature on natural microflora of minced cod, salmony, pork and beef meat was studied. Pressure of 193 MPa at –20 °C caused reduction of total bacterial count in pork and beef meat by 1.1 and 0.6 log cycles, respectively, and by about 1.5 log cycles in fish meat. Under these conditions the psychrophilic and psychrotophic bacteria were below the detection limit (<10CFU/g of sample) in...
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Suppressors of lapC mutation identify new regulators of LpxC, which mediates the first committed step in lipopolysaccharide biosynthesis
PublicationGram-negative bacteria, such as Escherichia coli, are characterized by an asymmetric outer membrane (OM) with lipopolysaccharide (LPS) located in the outer leaflet and phospholipids facing the inner leaflet. E. coli recruits LPS assembly proteins LapB, LapC and LapD in concert with FtsH protease to ensure a balanced biosynthesis of LPS and phospholipids. We recently reported that bacteria either lacking the periplasmic domain of...
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High prevalence of Escherichia coli belonging to the B2+D phylogenetic group in inflammatory bowel disease
PublicationBACKGROUND: It is not clear which species of bacteria may be involved in inflammatory bowel disease (IBD). One way of determining which bacteria might be likely candidates is to use culture-independent methods to identify microorganisms that are present in diseased tissues but not in controls. AIMS: (1) To assess the diversity of microbial communities of biopsy tissue using culture-independent methods; (2) to culture the bacteria...
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Novel single-stranded DNA-binding protein from psyvhrophilic bacterium Psychrobacter arcticus
PublicationTo study the biochemical properties of SSB from Psychrobacter arcticus (ParSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 642 nucleotides encoding SSB protein of 213 amino acids with a calculated molecular mass of 22.8 kDa. The amino-acid sequence of ParSSB exhibits 49% identity and 57% similarity to Escherichia coli SSB....
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Synthesis of green benzamide-decorated UiO-66-NH2 for biomedical applications
PublicationMetal-organic frameworks (MOFs) biocompatible systems can host enzymes/bacteria/viruses. Herein we synthesized a series of fatty acid amide hydrolase (FAAH)-decorated UiO-66-NH2 based on Citrus tangerine leaf extract for drug delivery and biosensor applications. Five chemically manipulated FAAH-like benzamides were localized on the UiO-66-NH2 surface with physical interactions. Comprehensive cellular and molecular analyses were...
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REVIEW ON COMPETITION АОB AND NОВ AS AN IMPORTANT ELEMENT FOR COST EFFECTIVE METHODS OF REMOVING NITROGEN FROM WASTEWATER
PublicationThe aim of this paper is analysis of process principles and possible factors used to induce inhibition of nitrite oxidation by NOB (Nitrite Oxidizing Bacteria) which could be obtained during not very well understood processes: so called “shortcut” or “partial” nitrification as well deammonification, by qualitative/quantitative studies of AOB (Ammonium Oxidizing Bacteria) and NOB populations in activated...
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Insights into oral microbiome and colorectal cancer – on the way of searching new perspectives
PublicationMicrobiome is a keystone polymicrobial community that coexist with human body in a beneficial relationship. These microorganisms enable the human body to maintain homeostasis and take part in mechanisms of defense against infection and in the absorption of nutrients. Even though microbiome is involved in physiologic processes that are beneficial to host health, it may also cause serious detrimental issues. Additionally, it has been...
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Ribes nigrum L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles
PublicationSilver nanoparticles (Ag NPs) represent one of the most widely employed metal-based engineered nanomaterials with a broad range of applications in different areas of science. Plant extracts (PEs) serve as green reducing and coating agents and can be exploited for the generation of Ag NPs. In this study, the phytochemical composition of ethanolic extract of black currant (Ribes nigrum) leaves was determined. The main components...
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Inhibition effect of free ammonia on deammonification process under different intermittent aeration strategies in sequencing batch reactor
PublicationThe deammonification process involves two steps. The first step is the partial nitrification of ammonia (NH4-N) by ammonia oxidizing bacteria (AOB) to produce nitrite (NO2-N) and the second step is the anammox process to “anaerobically” oxidize ammonia to nitrogen gas with nitrite as an electron acceptor (Lackner et al., 2014). Free ammonia (FA) plays a significant role in the stable, long-term deammonification system operation....
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Zastosowanie testu biologicznego Microtox do oceny skuteczności działania oczyszczalni ścieków bytowo-przemysłowych
PublicationOcena efektywności procesu oczyszczania ścieków z zastosowaniem biotestu Microtox® na przykładzie wybranej oczyszczalni Powszechnie praktykowanym sposobem wprowadzania ścieków oczyszczonych do środowiska jest ich punktowy zrzut do wód powierzchniowych. Różnorodność działalności prowadzonej w ramach współczesnej gospodarki sprawia, że do oczyszczalni docierają nie tylko substancje, których oznaczanie jest wymagane przez przepisy...
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MutS3: a MutS homologue of unknown biological function
PublicationThe homologues of MutS proteins are widespread among both Prokaryotes and Eukaryotes. MutS designated as MutS1 is a part of MMR (mismatch repair) system which is responsible for removal of mispaired bases and small insertion/deletion loops in DNA. Initially, the only MutS homologues known were those engaged in mismatch repair and these were later designated as MutS1. Subsequently, the MutS2 homologue was distinguished. MutS2 does...
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Pharmacomicrobiomics of cell-cycle specific anti-cancer drugs – is it a new perspective for personalized treatment of cancer patients?
PublicationIntestinal bacteria are equipped with an enzyme apparatus that is involved in the active biotrans-formation of xenobiotics, including drugs. Pharmacomicrobiomics, a new area of pharmacology, analyses interactions between bacteria and xenobiotics. However, there is another side to the coin. Pharmacotherapeutic agents can significantly modify the microbiota, which consequently affects their efficacy. In this review, we comprehensively...
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Pre-treatment of bio fraction waste prior to fermentation processes
PublicationCurrent efforts are taken to increase resource efficiency, close material loops, and improve sustainable waste and by-products management. Thus, networking agro-food by-products and converting them into valuable products completely exhausting the potential of the raw material becomes significant. Model lignocellulosic and starch based biomass were subjected to pre-treatment with the application of acidic compounds, i.e. sulphuric...
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Synthesis of amides under microwave irradiation
PublicationAmides belong to the most important carboxylic acid derivatives. They are constituents of natural compounds like peptides and proteins. They found applications in many branches of science and industry, especially in pharmaceutical and polymer science. Conventional procedures for amide preparation involve reaction between amine and carboxylic acid or their more active derivatives such as acid chlorides, acid anhydrides or esters....
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Comparison of nitrogen and VFA removal pathways in autotrophic and organotrophic anammox reactors
PublicationOrganotrophic anammox is a promising process for treating both nitrogen and organic containing wastewater than that of the traditional autotrophic anammox for sole nitrogen removal. However pathways of nitrogen removal particularly at metagenomic level in both processes are still unknown. Here we report, metabolic pathways of nitrogen removal in two lab-scale sequencing batch reactors (SBR), one autotrophic and another organotrophic...
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Preparation, composition and properties of fish silage produced with post-coagulation sludge
PublicationPost-coagulation sludge was added to fish fydrolyzates (silage). Silage was prepared using whole sprats. Technology of the process has been modified and sulphuric acid was replaced with phosphoric acid. The objective was to evaleuate the quality of produced silage during storage, particulary the changes of peroxide value and amount of free acids in silage fat.
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Soil and groundwater fecal contamination as a result of sewage sludge land application.
PublicationThe release of sludge-born bacteria and their further transport in subsurface was studied. The migration of bacteria was investigated in column experiments which were carried out under the conditions corresponding to naturally occurring extreme rainfall. Coal fly ash as well as coarse and medium grained sand, which properties are similar to the soils present in degraded areas, were used as column beds. Sewage sludge was applied...
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Seagrass vegetation and meiofauna enhance the bacterial abundance in the Baltic Sea sediments (Puck Bay)
PublicationThis study presents the first report on bacterial communities in the sediments of eelgrass (Zostera marina) meadows in the shallow southern Baltic Sea (Puck Bay). Total bacterial cell numbers (TBNs) and bacteria biomass (BBM) assessed with the use of epifluorescence microscope and Norland’s formula were compared between bare and vegetated sediments at two localities and in two sampling summer months. Significantly higher TBNs and...
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Biofilm bakteryjny uropatogennych szczepów E. coli Dr+ jako czynnik indukujący przewlekłość zakażeń dróg moczowych ograniczający ich leczenie
PublicationZakażenia dróg moczowych (ZUM) stanowią jedne z najczęściej występujących infekcji bakteryjnych, dotykających każdego roku miliony osób na świecie. Problematyka tych zakażeń wynika z ich przewlekłości i nawrotów, pomimo stosowania terapii antybiotykowej oraz ciągle wzrastającej lekooporności uropatogenów je wywołujących. Dominującym czynnikiem etiologicznym ZUM są uropatogenne szczepy E. coli (UPECs), wykazujące zdolność do adhezji,...
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Cloning, expression, purification and characterization of recombinant trehalose synthase from Deinococcus radiodurans.
PublicationTrehalose (α-D-glucopyranosyl-1,1-α-D-glucopyranoside) is a nonreducing disacharide in which the two glucose molecules are linked trough a α-1,1-glycosidic bond. Trehalose is readily hydrolyzed to glucose and can be used as a reserve of that sugar in the cell. The presence of trehalose was found in the cells of fungi and yeasts, bacteria, nematodes, insects, eggs, pupae and some plants. The characteristics of trehalose make it...
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IMPACT OF DIRECT CURRENT ON NITRIFYING BIOMASS
PublicationEffects of short term exposure of nitrifying biomass to direct current were studied. DC was applied to mixed liquor from municipal BNR plant using graphite electrodes in no substrate limiting conditions. Both, ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) populations were affected by the current, however in opposite ways. The AOB population activity was inhibited by applied voltage. Activity decrease was...
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Gold nanocubic structures functionalization with organic layers
Open Research DataThis dataset contains the XPS spectra obtained for gold nanocubes (AuNC), which were deposited at the gold electrode surface and dried. The AuNC's were first functionalized with either cysteamine in EtOH or mercaptopropionic acid in EtOH. The concentration of these compounds was 20 mM. The interaction of the functionalized with the AuNC surface was...
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The effect of raw material type on endotoxin content .
Open Research DataThe datasets contain the results of determining the effect of raw material type on endotoxin content as well as the effect of purification methods. The endotoxin concentration was determined using the PyroGene Recombinant Factor C Endpoint Fluorescent Assay. Beckmann-Kenko sodium alginate and Chemat sodium salt of alginic acid were used for the assays....
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Versatility of putative aromatic aminotransferases from Candida albicans.
PublicationAmino acids constitute the key sources of nitrogen for growth of Candida albicans. In order to survive inside the host in different and rapidly changing environments, this fungus must be able to adapt via its expression of genes for amino acid metabolism. We analysed the ARO8, ARO9, YER152C, and BNA3 genes with regards to their role in the nutritional flexibility of C. albicans. CaAro8p is undoubtedly the most versatile enzyme...
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Isolation and properties of recombinant trehalose synthase
PublicationBiosynthesis of trehalose is carried out via various matabolic pathways, including the use of trehalose synthase. Bacteria of the genus Deionococcus are microorganisms which produce trehalose synthase catalizing conversion of maltose into trehalose. In this work recombinant trehalose synthase from D.radiodurans was characterized.
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Peptidoglycan hydrolases - potential weapons against Staphylococcus aureus
PublicationBacteria of the genus Staphylococcus are common pathogens responsible for a broad spectrum of human and animal infections and belong to most important etiological factors causing food poisoning. Because of rapid increase in prevalence of isolation of staphylococci resistant to many antibiotics, there is an urgent need for development of new alternative chemotherapeutics. A number of studies have recently demonstrated the strong...
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A shear stress micromodel of urinary tract infection by the Escherichia coli producing Dr adhesin
PublicationIn this study, we established a dynamic micromodel of urinary tract infection to analyze the impact of UT-segment-specific urinary outflow on the persistence of E. coli colonization. We found that the adherence of Dr+ E. coli to bladder T24 transitional cells and type IV collagen is maximal at lowest shear stress and is reduced by any increase in flow velocity. The analyzed adherence was effective in the whole spectrum of physiological...
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Thermal Decomposition of Carbamoyl Meldrum’s Acids: A Starting Point for the Preparation of 1,3-Oxazine Derivatives
PublicationThe ability to undergo [4 + 2] versus [2 + 2] cycloaddition was under investigation for ketenes thermally generated from carbamoyl Meldrum’s acid. Usually, 1,3-oxazino-5-carbamoylo-4,6-diones are formed when carbamoyl Meldrum’s acid reacts with imine. However, in some cases, a reaction takes an unexpected course, leading to the formation of tetraponerines alkaloids derivatives or cyclic iminoethers.
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Effect of choline chloride based natural deep eutectic solvents on aqueous solubility and thermodynamic properties of acetaminophen
PublicationIn this work, natural deep eutectic solvents (NADESs) containing choline chloride as hydrogen bond acceptor and 1,2-propanediol, malic acid and tartaric acid as hydrogen bond donors have been synthesized and applied to enhance the aqueous solubility of model sparingly water-soluble drug – acetaminophen. The results indicate that the greatest impact on the solubility of acetaminophen have deep eutectic solvents based on 1,2-propanediol...
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Bee Products and Essential Oils as Alternative Agents for Treatment of Infections Caused by S. aureus
PublicationBacteria of the genus Staphylococcus are important human and veterinary pathogens. A crucial characteristic for this group of bacteria is that they can easily acquire mechanisms of antibiotic resistance for a plethora of antibiotics currently in use for human and animal therapies. Therefore, there is a great need to find novel, non-antibiotic chemotherapeutics with marked antistaphylococcal activity. Promising but still underestimated...
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Detection of UPEC IH11128 ability to form biofilm in a standard congo red (CR) method of colony staining.
Open Research DataA standard method of testing E. coli's ability to form biofilm is based on the analysis of morphologyof bacterial colonies grown on yeast extract/casamino acids (YESCA) nutrient agar medium containing congored (CR) as an indicator dye of ECM production. The CR dye adsorbed from the culture medium bybacteria binds to the components of the ECM, including...
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Degradation of xylose using a microbial fuel cell
PublicationIt is generally known, that many kind of microorganisms are capable of using carbohydrates as a source of carbon and energy in an environment. The biodegradation process of monosaccharides from pentoses (in this case- racemic mixture of D - and L – xylose) is an immense opportunity for an entire reduction of biological contamination to neutral compounds through MFC. The process is occurred in a single-chamber MFC, which is contained...
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The Physicochemical and Antibacterial Properties of Chitosan-Based Materials Modified with Phenolic Acids Irradiated by UVC Light
PublicationThis paper concerns the physicochemical properties of chitosan/phenolic acid thin films irradiated by ultraviolet radiation with wavelengths between 200 and 290 nm (UVC) light. We investigated the preparation and characterization of thin films based on chitosan (CTS) with tannic (TA), caffeic (CA) and ferulic acid (FA) addition as potential food‐packaging materials. Such materials were then exposed to the UVC light (254 nm) for...
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Comparison of disk and tube methods for estimation of biostatic properties of polyurethanes based on synthetic poly[(R,S)-3-hydroxybutyrate
PublicationThe influence of novel aliphatic polyurethanes on growth of Staphyloccocus aureus (SA)in the around of polymer samples has been estimated by disk method. It was staed that the growth of SA was inhibited.The influence of polyurethanes on planctonic Staphyloccocus aureus was also pefrormed by tube method. In this test the slight decrease of bacteria concentration was noticed.
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Investigation of sour substances by five-channel potentiometric taste sensor containing all-solid-state-electrodes ( ASSEs)
PublicationAn elaboration of taste sensor for discrimination of different food products is of great importance for food industry. Potentiometric taste sensor containing ion selective electrodes with lipid/polymer membranes has already been applied commercially in food industry. However, time-consuming and demanding preconditioning method of ions selective electrodes as well as maintenance of electrodes’ bodies are disadvantages of this taste...
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The role of microbial coagulants on the physicochemical, proteolysis, microstructure and sensory properties of low-fat Edam cheese manufactured from ultrafiltered buffalo milk
PublicationThis work investigates the influence of using microbial coagulants, including Rhizomucor miehei (MCR) protease and Cryphonectria parasitica (MCC) protease, on the quality characteristics of low-fat Edam cheese made from ultrafiltered buffalo milk (LFUE). Concurrently, a benchmark with calf rennet (CR) has been also performed. Throughout a 90-day ripening period, the cheeses were assessed for their physicochemical features, proteolysis,...
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INVESTIGATION OF SOUR SUBSTANCES BY FIVE-CHANNEL POTENTIOMETRIC TASTE SENSOR CONTAINING ALL-SOLID-STATE- ELECTRODES (ASSEs)
PublicationAn elaboration of taste sensor for discrimination of different food products is of great importance for food industry. Potentiometric taste sensor containing ion selective electrodes with lipid/polymer membranes has already been applied commercially in food industry. However, time-consuming and demanding preconditioning method of ions selective electrodes as well as maintenance of electrodes’ bodies are disadvantages of this taste...
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BET adsorption-desorption isotherm of CeO2 prepared using Pechini method
Open Research DataThe dataset includes the isotherms of adortion and desorption of N2 at LN2 temperature. The sorption isotherms were obtained using Quatachrome NovaTouch LX1 equippment. The ceramic measured powder was composed of nanometric pure CeO2 prepared using modified Pechini method - citric acid and propylene glycol (2:1)
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Waterborne polyesters partially based on renewable resources
PublicationA new experimental approach for preparing biobased, water-soluble polyesters via titanium(IV) n-butoxide-catalyzed bulk polycondensation is presented. In the described method polymers were obtained from isosorbide, maleic anhydride and poly(ethylene glycol). The chemical structure of the synthesized polyesters was confirmed using 2D NMR spectroscopy and by titration methods. Careful analysis of 2D NMR spectra viz. Correlation Spectra...
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A comprehensive lipidomic analysis of oilseeds using LC-Q-TOF-MS and dispersive micro-solid phase (D-μ-SPE) extraction techniques
PublicationGrowing public health awareness increased the demand for natural products with positive health benefits. Vegetable oils containing omega-3 fatty acids (FA) such as alpha-linolenic acid (ALA) has been proposed as a possible source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). In this work, a comprehensive lipidome analysis and comparison of seven oilseeds, including flaxseed, chia, hemp, black cumin, white sesame,...
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Polymer biodegradable coatings as active substance release systems for urological applications
PublicationCatheter-associated urinary tract infections are the result of catheterization of the bladder. The risk of infection is directly proportional to the length of the bladder catheterization. Bacteria cells have the ability to adhere and create the biofilm on the surface of catheter materials. Bacteria’s biofilm is an extremely beneficial environment of existence for microorganisms. Microorganisms that are an integral part of the biofilm...
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Chracteristics of anaerobic ammonium oxidation initated by anaerobic granular sludge
PublicationTwo different mixed sludge, anaerobic granular sludge(R1) and anaerobic granular sludge with mature anaerobic ammonium oxidation (ANAMMOX) sludge (R2 )were used as the inocula in two up-flow reactors to enrich anaerobic ammonia-oxidizing bacteria (AnAOB),respectively.The total nitrogen removal rate of R1 reached 74% in 99 days, while R2 remained above 70% in only 48 days.The content of extracellular polymeric substance (EPS) in...
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Direct determination of cadaverine in the volatile fraction of aerobically stored chicken breast samples
PublicationTo supplement the currently used methods for poultry meat shelf life assessment, it might be necessary to develop a technique for rapid headspace analysis of volatiles with no prior sample preparation step. Biogenic amines, in particular cadaverine, are considered meat spoilage indicators. Described in this article are the results of a preliminary investigation of the applicability of proton transfer reaction mass spectrometry...
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Testing of DES toxicity towards Candida subhashii
Open Research DataDataset presents results of testing toxicity of selected DES solutions in mineral salt medium towards Candida subhashii.
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Synthesis and biological evaluation of novel analogues of batracylin with synthetic amino acids and adenosine: an unexpected effect on centromere segregation in tumor cells through a dual inhibition of topoisomerase IIa and Aurora B
PublicationIn the search for new anticancer agents we designed and synthesized batracylin derivatives with linking synthetic amino acid side chains of different lengths and adenosine. Unexpectedly, we have found that in water and the culture media adenosine–amino acid–BAT conjugates form supramolecular structures and this prevents these compounds from entering cells. Consequently, these compounds exerted no biological activity when tested...