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Wyniki wyszukiwania dla: IMMUNOLOGICAL AGENT
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Enhanced photocatalytic activity of accordion-like layered Ti3C2 (MXene) coupled with Fe-modified decahedral anatase particles exposing {1 0 1} and {0 0 1} facets
PublikacjaNew composites consisting of decahedral anatase particles exposing {001} and {101} facets coupled with accordion-like layered Ti3C2 with boosted photocatalytic activity towards phenol and carbamazepine degradation were investigated. The photocatalysts were characterized with X-ray diffraction (XRD), diffuse reflectance spectroscopy (DR/UV–Vis), Brunauer-Emmett-Teller (BET) specific surface area, Raman spectroscopy, scanning electron...
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Bioreactor shoot cultures of Rhododendron tomentosum (Ledum palustre) for a large-scale production of bioactive volatile compounds
PublikacjaRhododendron tomentosum Harmaja (Ledum palustre), a peat bog plant from Ericaceae family, has been used in traditional medicine as the anti-arthritis agent. Although modern researches confirm its anti-inflammatory properties, it remains threatened by habitat degradation and possibilities to collect this endangered species from its natural environment for further biological activity studies are limited. Therefore, R. tomentosum...
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Visible light photoactivity of TiO2loaded with monometallic(Au or Pt) and bimetallic (Au/Pt) nanoparticles
PublikacjaTiO2modified with monometallic (Au or Pt) and bimetallic (Au/Pt) nanoparticles have been preparedusing a water-in-oil microemulsion system (water/AOT/cyclohexane) followed by calcination step. Theeffect of metal ratio, reducing agent type (NaBH4or N2H4), TiO2matrix type (P-25, ST-01, TiO-5, TiO2nano-tubes or TiO2obtained by TIP hydrolysis) as well as calcination temperature (from 350 to 650◦C) weresystematically investigated. Obtained...
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Optical properties of the chemotherapy drugs used in the central nervous system lymphoma therapy: monitoring drug delivery
PublikacjaOur aim is to optically monitor the delivery of the chemotherapy drugs for brain tumours, particularly used in the central nervous system (CNS) lymphoma therapy. In vivo monitoring would help to optimize the treatment and avoiding unnecessary medications. Moreover, it would be beneficial to be able to measure which of the multi-regimen drugs actually do penetrate and how well into the brain tissue. There exist several potential...
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CaZnO-based nanoghosts for the detection of ssDNA, pCRISPR and recombinant SARS-CoV-2 spike antigen and targeted delivery of doxorubicin
PublikacjaOverexpression of proteins/antigens and other gene-related sequences in the bodies could lead to significant mutations and refractory diseases. Detection and identification of assorted trace concentrations of such proteins/antigens and/or gene-related sequences remain challenging, affecting different pathogens and making viruses stronger. Correspondingly, coronavirus (SARS-CoV-2) mutations/alterations and spread could lead to overexpression...
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Controlling the europium oxidation state in diopside through flux concentration
PublikacjaThis paper explores the connection between the H3BO3 flux concentration and the co-existence of Eu2+ and Eu3+ dopants within CaMgSi2O6 crystals (diopside). The samples were synthesised using a solid-state synthesis method under varying atmospheric conditions, including oxidative (air), neutral (N2), and reductive (H2/N2 mixture) environments. Additionally, some materials underwent chemical modification by partially substituting...
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Quality control of cheese samples for the presence of natamycin preservative – A natural deep eutectic solvent (NADES) based extraction coupled with HPLC
PublikacjaA new protocol for the determination of natamycin – an antifungal agent used as a food preservative - in cheese samples – is described. This new method is based on a natural deep eutectic solvent (NADES) green extraction procedure. High-performance liquid chromatography (HPLC) was used for detection and quantification. NADESs with different molar ratios were evaluated for efficient and selective extraction. NADES made of thymol...
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Pilicides effectively hinder chaperone-usher interactions in all Gram-negative bacteria
PublikacjaThe chaperone-usher pathway of adhesive structures biogenesis is highly conserved in all Gram-negative bacteria. Pili are polymers of thousands protein subunits that possess conserved immunoglobuline-like structure denoted by lack of the seventh G strand. The effect of this structural defect is a hydrophobic acceptor cleft. The folding of protein subunits is strictly dependent on the action of specific periplasmic chaperone protein...
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Ultrasound assisted synthesis of 3-alkynyl substituted 2-chloroquinoxaline derivatives: Their in silico assessment as potential ligands for N-protein of SARS-CoV-2
PublikacjaIn view of recent global pandemic the 3-alkynyl substituted 2-chloroquinoxaline framework has been explored as a potential template for the design of molecules targeting COVID-19. Initial in silico studies of representative compounds to assess their binding affinities via docking into the N-terminal RNA-binding domain (NTD) of N-protein of SARS-CoV-2 prompted further study of these molecules. Thus building of a small library of...
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Optimized 5-hour multiplex PCR test for the detection of tinea unguium: performance in a routine PCR laboratory
PublikacjaWe recently reported the development of a 5-hour multiplex PCR test for the detection of tinea unguium and the optimization of this test by the inclusion of an inhibition control. Here we report the performance of this procedure as used in a routine clinical laboratory as compared to conventional microscopy and culture-based techniques performed in a mycology reference laboratory. We found in processing 109 samples that 22 (20.2%)...
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Using human behavior to develop knowledge-based virtual organisations
PublikacjaPropozycja modelu opartego na zachowaniach ludzkich dla potrzeb budowy wirtuanych organizacji opartych na wiedzy.Virtual organizations promote dynamic interaction between individuals, groups, and organizations, who share their capabilities and resources to pursue a common goal and maximize their benefits. Among these resources, knowledge is a critical one that requires special attention in order to support problem-solving activities...
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Transport of paracetamol in swellable and relaxing polyurethane nanocomposite hydrogels
PublikacjaPolyurethane hydrogels are potentially attractive materials for biomedical applications. They are able to absorb large amount of water, biological fluids or active substances, and thus, they have potential to be used as absorbents or wound-healing dressings. They are also used for the controlled release of therapeutics because of their capacity to embed biologically active agents in their water-swollen network. The presence of...
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Gold nanoparticles evaluation using functional optical coherence tomography
PublikacjaThe main object of this research was to assess the ability to characterize the gold nanoparticles using optical modalities like optical coherence tomography. Since the nanoparticles, especially gold one, have been very attractive for medical diagnosis and treatment the amount of research activities have been growing rapidly. The nanoparticles designed for different applications like contrast agents or drugs delivery change the...
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Alternative Methods of Preparation of Soluble Keratin from Chicken Feathers
PublikacjaHuge amount of keratinous waste, especially birds’ feathers, demands more value-added application instead of dumping. The present work reports the results of experiments aimed at preparing soluble keratin useful for novel bioproduct formation. The effect of thermo-chemical treatments with various reducing agents, i.e. 2-mercaptoethanol, dithiothreitol, sodium m-bisulphite, and sodium bisulphite, as well as sodium hydroxide, on...
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Fabrication of wormhole-like YSZ and Ni-YSZ by the novel soft-hard template CTAB/NaCl-assisted route. Suppressing Ni coalescence in SOFC
PublikacjaA novel one-pot synthesis route leading to the formation of a wormhole-like structure was developed for the successful fabrication of porous YSZ and Ni-YSZ systems. This method involved co-precipitation in the presence of the micelle-forming agents CTAB/Pluronic P123 and crystallising NaCl. The obtained skeletons were mechanically stable and presented almost 50% uniform, open porosity without using any additional pore-formers....
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Missing Puzzle Pieces in Dementia Research: HCN Channels and Theta Oscillations
PublikacjaIncreasing evidence indicates a role of hyperpolarization activated cation (HCN) channels in controlling the resting membrane potential, pacemaker activity, memory formation, sleep, and arousal. Their disfunction may be associated with the development of epilepsy and age-related memory decline. Neuronal hyperexcitability involved in epileptogenesis and EEG desynchronization occur in the course of dementia in human Alzheimer’s Disease...
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The implementation of green transformation through clusters
PublikacjaThe paper addresses a poorly documented issue in the literature, namely the role of clusters in green transformation, including processes related to green, low-carbon, and circular economies. The purpose was to identify and understand the practices of clusters in this area. The adopted mixed research strategy consisted of both qualitative and quantitative research. Both research phases were conducted in a group of Polish Key National...
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Tomasz Szymański dr inż. arch.
OsobyTomasz Szymański (dr inż. arch.) jest architektem i adiunktem na Wydziale Architektury Politechniki Gdańskiej. Absolwent Wydziału Architektury Politechniki Gdańskiej. Studia zakończone dyplomem z wyróżnieniem. Zainteresowania badawcze dr. Tomasza Szymańskiego skoncentrowane są wokół kwestii dziedzictwa przemysłowego, re-use, adaptacji i rewitalizacji obiektów poprzemysłowych, obiektów poportowych i postoczniowych na nowe funkcje...
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BINDER COURSES USING COLD RECYCLED MIXTURES – A NOVEL CONCEPT IN COLD RECYCLING
PublikacjaCold recycling with cement and bituminous emulsion is one of the most environmentally friendly techniques to maximize the reuse of reclaimed asphalt (RA) collected during demolition of roads. Cold recycled mixtures are commonly used for base courses in construction or rehabilitation of flexible and semi-rigid pavements. Current experiences demonstrated that cold recycling with appropriate requirements and technical recommendations...
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Nanocrystalline diamond sheets as protective coatings for fiber-optic measurement head
PublikacjaFiber-optic sensors find numerous applications in science and industry, but their full potential is limited because of the risk of damaging the measurement head, in particular, due to the vulnerability of unprotected tips of the fiber to mechanical damage and aggressive chemical agents. In this paper, we report the first use of a new nanocrystalline diamond structure in a fiber-optic measurement head as a protective coating of...
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Comparative Assessment of Bacteriophage and Antibiotic Activity against Multidrug-Resistant Staphylococcus aureus Biofilms
PublikacjaProblems connected with biofilm-related infections and antibiotic resistance necessitate the investigation and development of novel treatment strategies. Given their unique characteristics, one of the most promising alternatives to conventional antibiotics are bacteriophages. In the in vitro and in vivo larva model study, we demonstrate that phages vB_SauM-A, vB_SauM-C, and vB_SauM-D are effective antibiofilm agents. The exposure...
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Modelling of heat transfer in supercritical pressure recuperators
PublikacjaIn the paper presented is analysis of convective flow heat transfer at supercritical pressure in channels of heat exchanger working in the thermodynamic cycle. The modelling is based on the division of the flow into three regions, namely the heavy fluid, a two phase flow consisting of the heavy and light fluids and finally the light fluid flow. Modelling is concentrated on the region of simultaneous flow of two fluids divided into...
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Analytics of Surfactants in the Environment: Problems and Challenges
PublikacjaSurfactants (surface active agents = SAAs) are a group of compoundswith specific physicochemical properties (amphiphilicity,solubility in polar and nonpolar liquids, ability to form micelles,adsorption at phase boundaries).1,2 Because of their properties,surface-active compounds are widely applied in industry and thehousehold (e.g., in detergents, personal-care products, paints,pesticides, petroleum products). As their applications...
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Use of Escherichia coli Nissle 1917 producing recombinant colicins for treatment of IBD patients
PublikacjaPatients with Crohn’s Disease and ulcerative colitis infected with Adherent-Invasive Escherichia coli strains constitute the largest group among Inflammatory Bowel Disease subjects, when taking into account all known etiological agents of the disease. A possible link between these pathogenic bacteria and inflammation process has gained the confidence in recently published papers. Observed enteric neuroglial cells apoptosis and...
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Tetrahydroquinolinone derivatives exert antiproliferative effect on lung cancer cells through apoptosis induction
PublikacjaThe anticancer properties of quinolones is a topic of interest among researchers in the scientific world. Because these compounds do not cause side effects, unlike the commonly used cytostatics, they are considered a promising source of new anticancer drugs. In this work, we designed a brief synthetic pathway and obtained a series of novel 8-phenyltetrahydroquinolinone derivatives functionalized with benzyl-type moieties at position...
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Development of Safirinium dyes for new applications: fluorescent staining of bacteria, human kidney cells, and the horny layer of the epidermis
PublikacjaLow-molecular synthetic fluorophores are convenient tools in bioimaging applications. Several derivatives of Safirinium dyes as well as their reactive N-hydroxysuccinimide (NHS) esters bearing diverse substituents were synthesized and evaluated experimentally in terms of their lipophilicity by means of reverse-phase and immobilized artificial membrane high-performance liquid chromatography. Subsequently, the selected compounds...
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Anticarcinogenic and antioxidant activities of leaves and flowers hydroalcoholic extracts of Nerium oleander L.: PCA analysis and phytochemical content by FTIR spectroscopy
PublikacjaAims: The aim of this work is the evaluation the anticarcinogenic effect on HT29 cancer cells lines and antioxidant effect of three extracts from different morphological parts (leaves (LE), pink flowers (PFE) and white flowers (WFE)) of Nerium oleander lin. Methods: This research provides the anticarcinogenic activity of extracts from N. oleander white flowers. Principal Component Analysis (PCA) was applied to estimate the relationship...
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Morphology control through the synthesis of metal-organic frameworks
PublikacjaDesignable morphology and predictable properties are the most challenging goals in material engineering. Features such as shape, size, porosity, agglomeration ratio significantly affect the final properties of metal- organic frameworks (MOFs) and can be regulated throughout synthesis parameters but require a deep under- standing of the mechanisms of MOFs formation. Herein, we systematically summarize the effects of the indi- vidual...
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Conversion of waste biomass into activated carbon and evaluation of environmental consequences using life cycle assessment
PublikacjaIn this article, activated carbon was produced from Lantana camara and olive trees by H3PO4 chemical activation. The prepared activated carbons were analyzed by characterizations such as scanning electron microscopy, energy-dispersive X-ray spectroscopy, Brunauer–Emmett–Teller, X-ray diffraction, thermogravimetric analysis, and Fourier transform infrared spectroscopy. H3PO4 is used as an activator agent to create an abundant pore...
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Honey antibacterial activity: A neglected aspect of honey quality assurance as functional food
PublikacjaBackground Honey is considered as a functional food with health-promoting properties. Its potent antibacterial and antibiofilm effects are the major attributes of so called ‘medical-grade honey’ which is topically used for the treatment of burns, wounds and skin disorders. Nevertheless, the current set of honey quality parameters adopted in the European Union do not include its biological properties. Furthermore, in light of the...
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Acetate-Induced Disassembly of Spherical Iron Oxide Nanoparticle Clusters into Monodispersed Core−Shell Structures upon Nanoemulsion Fusion
PublikacjaIt has been long known that the physical encapsulation of oleic acid-capped iron oxide nanoparticles (OA−IONPs) with the etyltrimethylammonium (CTA+) surfactant induces the formation of spherical iron oxide nanoparticle clusters (IONPCs). However, the behavior and functional properties of IONPCs in chemical reactions have been largely neglected and are still not well-understood. Herein, we report an unconventional ligand-exchange...
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Synthesis, physicochemical and theoretical studies on new rhodium and ruthenium dimers. Relationship between structure and cytotoxic activity
PublikacjaTwo dimeric compounds of the general formulae (Et3NH)2[Rh2(μ2-L)4Cl2] (1) (where L = thiophene-2-carboxylate) and [((η6-p-cymene)Ru)2(μ-Cl)3]PF6 (2) have been synthesized using a new method. The unique anionic complex 1 (space group P bca) has octahedral coordination in which the equatorial positions are occupied by the oxygen atoms of four thiophene-2-carboxylates in a paddle wheel fashion. In complex 2 (space group ), each Ru(II)...
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Polyurethanes Crosslinked with Poly(vinyl alcohol) as a Slowly-Degradable and Hydrophilic Materials of Potential Use in Regenerative Medicine
PublikacjaNovel, slowly-degradable and hydrophilic materials with proper mechanical properties and surface characteristics are in great demand within the biomedical field. In this paper, the design, synthesis, and characterization of polyurethanes (PUR) crosslinked with poly(vinyl alcohol) (PVA) as a new proposition for regenerative medicine is described. PVA-crosslinked PURs were synthesized by a two-step polymerization performed in a solvent...
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Application of safirinium N-hydroxysuccinimide esters to derivatization of peptides for high-resolution mass spectrometry, tandem mass spectrometry, and fluorescent labeling of bacterial cells
PublikacjaMass spectrometry methods are commonly used in the identification of peptides and biomarkers. Due to a relatively low abundance of proteins in biological samples, there is a need for the development of novel derivatization methods that would improve MS detection limits. Hence, novel fluorescent N-hydroxysuccinimide esters of dihydro-[1,2,4]triazolo[4,3-a]pyridin-2-ium carboxylates (Safirinium P dyes) have been synthesized. The...
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Jak wykraść złoto smokowi? - uczenie ze wzmocnieniem w świecie Wumpusa
PublikacjaNiniejszy rozdział zawiera łagodne wprowadzenie do problematyki uczenia ze wzmocnieniem, w którym podstawy teoretyczne wyjaśniane są na przykładzie przewodnim, jakim jest zagadnienie nauczenia agenta poruszania się w świecie potwora o imieniu Wumpus (ang. Wumpus world), klasycznym środowisku do testowania logicznego rozumowania agentów (problem nietrywialny dla algorytmów uczenia ze wzmocnieniem). Przedstawiona jest główna idea...
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Unsaturated, biobased polyesters and their cross-linking via radical copolymerization
PublikacjaBiobased, unsaturated polyesters derived from isosorbide, maleic anhydride and succinic acid were synthesized and characterized. The presence of maleic anhydride units in the structure of the polyesters allowed converting them into cured coatings by radical copolymerization with cross-linking agents such as 2-hydroxyethyl methacrylate, N-vinyl-2-pyrrolidinone, acrylic acid or methacrylamide. The investigated polyesters were obtained...
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Treatment innovation using solar/UV In book: The Treatment of Pharmaceutical Wastewater: Innovative Technologies and the Adaptation of Treatment Systems
PublikacjaSince ancient times, sunlight irradiation has been widely used for water purification. Apart from solar water disinfection techniques, UV irradiation is of particular interest when considering the photodegradation of various classes of emerging pollutants. Pharmaceutically active compounds are one of the largest classes of micropollutants. The main sources of these contaminants in wastewater and aqueous environments are households,...
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H2AX phosphorylation, its role in DNA damage response and cancer therapy
PublikacjaDouble-strand breaks (DSBs) are the most deleterious DNA lesions, which, if left unrepaired, may have severe consequences for cell survival, as they lead to chromosome aberrations, genomic instability, or cell death. Various physical, chemical, and biological factors are involved in DSB induction. Cells respond to DNA damage by activating the so-called DNA damage response (DDR), a complex molecular mechanism developed to detect...
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Surfactants: a real threat to the aquatic geoecosystems of lobelia lakes
PublikacjaLobelia lakes are valuable elements of the natural environment. They are characterised by low trophy, mainly in-forest location and a high transparency of water. However, similarly to other surface waters, they are subjected to increasing anthropogenic pressures, a good indicator of which is the level of surfactants, also called surface-active agents (SAAs). The aim of the study was to evaluate the intensity of anthropogenic pressures...
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PCR and real-time PCR assays to detect fungi of Alternaria alternata species
PublikacjaFungi of the Alternaria genus are mostly associated with allergic diseases. However, with a growing number of immunocompromised patients, these fungi, with A. alternata being the most prevalent one, are increasingly recognized as etiological agents of infections (phaeohyphomycoses) in humans. Nowadays, identification of Alternaria spp. requires their pure culture and is solely based on morphological criteria. Clinically, Alternaria...
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The influence of pre- and postharvest treatments on selected biological and epigenetic activities of Brassica sprouts
PublikacjaIntroduction The Brassica vegetables are rich in glucosinolates (GLs), which are enzymatically hydrolysed to bioactive isothiocynates (ITC) and indoles, regarded as the most promising chemopreventing agents. However, in the majority of brassica sprouts the GL conversion into these beneficial derivatives occurs at the level of about 1 %, while in cabbage leaves exceeds 70%. Since sprouts represent plant material, which is frequently...
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Designing with Green and Blue – Climate Adaption Proposals for Lowland Areas of Gdańsk
PublikacjaThe paper gives insight into issues explored within the framework of the SOS Climate Waterfront workshop that took place in Gdańsk in June 2019. The aim of the study was to propose solutions that will decrease the number of flooding events and span the gap between flood prevention strategies and the provision of other benefits such as ecological, urban, cultural and social. Historical cartography enquiries and research by the design...
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MXenes Antibacterial Properties and Applications: A Review and Perspective
PublikacjaThe mutations of bacteria due to the excessive use of antibiotics, and generation of antibiotic-resistant bacteria have made the development of new antibacterial compounds a necessity. MXenes have emerged as biocompatible transition metal carbide structures with extensive biomedical applications. This is related to the MXenes’ unique combination of properties, including multifarious elemental compositions, 2D-layered structure,...
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Antimicrobial and Antiproliferative Coatings for Stents in Veterinary Medicine—State of the Art and Perspectives
PublikacjaMicrobial colonization in veterinary stents poses a significant and concerning issue in veterinary medicine. Over time, these pathogens, particularly bacteria, can colonize the stent surfaces, leading to various complications. Two weeks following the stent insertion procedure, the colonization becomes observable, with the aggressiveness of bacterial growth directly correlating with the duration of stent placement. Such microbial...
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Processing, Mechanical and Morphological Properties of GTR Modified by SBS Copolymers
PublikacjaIn this work, ground tire rubber (GTR) was thermo-mechanically treated in the presence of styrene-butadiene-styrene (SBS) copolymers. During preliminary investigation, the effects of different SBS copolymer grades, the variable content of SBS copolymer on the Mooney viscosity, and the thermal and mechanical properties of modified GTR were determined. Subsequently, GTR modified by SBS copolymer and cross-linking agents (sulfur-based...
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Modyfikacja cementu kostnego w celu uzyskania długotrwałej ochrony antybakteryjnej
PublikacjaNiniejsza rozprawa doktorska stanowi przewodnik po jednotematycznym cyklu publikacji, dotyczącym modyfikacji akrylowego cementu kostnego celem uzyskania długotrwałej i efektywnej ochrony antybakteryjnej. W ramach niego przeprowadzono badania eksperymentalne polegające na opracowaniu technologii wytwarzania modyfikowanego cementu kostnego oraz procedury medycznej jego stosowania. Cement kostny był modyfikowany przez zastosowanie...
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IDENTYFIKACJA CZYNNIKÓW WPŁYWAJĄCYCH NA BEZPIECZEŃSTWO EKSPLOATACJI STATKU NA ŚRÓDLĄDOWEJ DRODZE WODNEJ W DELCIE WISŁY
PublikacjaKażdego roku w Polsce, na śródlądowych drogach wodnych rejestrowane są przez Urzędy Żeglugi Śródlądowej, Państwową Straż Pożarną i Policję wypadki i incydenty, prowadzące zarówno do uszkodzenia infrastruktury jak i uszczerbku na zdrowiu. Poprawa poziomu bezpieczeństwa wymaga dogłębnej analizy i wyciągania wniosków z zaistniałych sytuacji awaryjnych. Istotną rzeczą jest identyfikacja i usystematyzowanie zagrożeń występujących w...
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Elementy psychologii w kontekście autonomii robotów
PublikacjaW niniejszym opracowaniu zaprezentowano szerokie spektrum zagadnień związanych z psychologią poznawczą w kontekście antropoidalnej robotyki. W szczególności omówiono sposób przetwarzania informacji u człowieka, od bodźca do reakcji, tak aby przedstawioną wiedzę można było wykorzystać przy projektowaniu robotów autonomicznych. Na początku pracy szczegółowo omówiono procesy poznawcze: percepcję sensoryczną, spostrzeganie,...
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Activation of endogenous regenerative potential in mammals using epigenetic inhibitor zebularine
PublikacjaIn this work, a nucleoside inhibitor of DNA methyltransferases, zebularine, was investigated as a wound healing and regeneration promoting agent. It was found that a high dose of intraperitoneally delivered zebularine improved punch wound closure in the ear pinna in mice. Both cytidine, a zebularine analogue, and uridine, a zebularine metabolite, did not promote ear pinna hole closure. The effect of zebularine on ear pinna healing...
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Assestment of the anti-cancer activity of the copper complexes with imidazole and pivalato ligands
PublikacjaDespite the development of science and technology progress so far there have not been found effective drugs for cancer. Many coordination compounds were investigated due to their antitumor potential. The most known cis-diamminedichloroplatinum(II) is used as anti-cancer therapeutic agent. The copper complexes are the coordination compounds possessing anti-tumour properties. Their capability to kill cancer cells is mainly linked...