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Search results for: folded%20proteins
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Saint-Venant torsion based on strain gradient theory
PublicationIn this study, the Saint-Venant torsion problem based on strain gradient theory is developed. A total form of Mindlin's strain gradient theory is used to acquire a general Saint-Venant torsion problem of micro-bars formulation. A new Finite Element formulation based on strain gradient elasticity theory is presented to solve the Saint-Venant torsion problem of micro-bars. Moreover, the problem is solved for both micro and macro...
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Multipath Complex Root Tracing
PublicationThe problem of multipath root tracing is being addressed in this communication. The self-adaptive complex root tracing algorithm, which was previously utilized for the investigation of various propagation and radiation problems, is analyzed here for the cases when the traced characteristic bifurcates. A procedure of multiroute detection is proposed and demonstrated on the coaxially loaded cylindrical waveguide example.
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Fast Algorithms for Identification of Time-Varying Systems with Both Smooth and Discontinuous Parameter Changes
PublicationThe problem of noncausal identification of a time-varying linear system subject to both smooth and occasional jump-type changes is considered and solved using the preestimation technique combined with the basis function approach to modeling the variability of system parameters. The proposed estimation algorithms yield very good parameter tracking results and are computationally attractive.
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Protein thermal stabilization in aqueous solutions of osmolytes
PublicationProteins’ thermal stabilization is a significant problem in various biomedical, biotechnological, and technological applications. We investigated thermal stability of hen egg white lysozyme in aqueous solutions of the following stabilizing osmolytes: Glycine (GLY), N-methylglycine (NMG), N,N-dimethylglycine (DMG), N,N,N-trimethylglycine (TMG), and trimethyl-N-oxide (TMAO). Results of CD-UV spectroscopic investigation were compared...
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Cell-penetrating peptides – mechanism of transduction and synthesis – short review
PublicationPotential antimicrobial agents may be effective if they are delivered efficiently to their site of action. In many cases, lack of permeability through the cell membrane is the main problem. One of the possible solutions could be a novel intracellular delivery system involving cell-penetrating peptides (CPPs), which may improve the efficiency of many new biophar-maceuticals. Among other things, CPPs are capable of trans-porting...
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Influence of the internal electrolyte composition on amperometric sulphur dioxide sensor properties
PublicationIn this work amperometric sulphur dioxide sensor with a liquid electrolyte, a mercury sulphate reference electrode and solid polymer electrolyte (Nafion) covered with gold as a working electrode will be presented. The main goal of this research is to determine the influence of internal electrolyte composition on the sensor properties. Sensor filled with 1M and 5M sulphuric acid has been investigated.
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Charakterystyka hydrogeochemiczna wód podziemnych rejonu Przylądka Rozewie na podstawie badań ich naturalnych wypływów
PublicationW niniejszym artykule zostały przedstawione wyniki badań, które były prowadzone w strefie brzegowej rejonu Przylądka Rozewie. Prowadzone w latach 2013-2014 badania miały charakter monitoringu podstawowych właściwości fizykochemicznych oraz oznaczenia składu jonowego wód wypływających ze zbocza w obrębie badanego terenu. Warunki hydrogeochemiczne rejonu Przylądka Rozewie są kształtowane pod wpływem specyficznego nadmorskiego klimatu,...
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Badania charakterystyki obciążeń oddziałujących na modułowe urządzenie dylatacyjne w pasie ruchu ciężkiego autostrady A8
PublicationPrzedstawiono badania urządzenia dylatacyjnego typu nożycowego. Dokonano statycznych i dynamicznych pomiarów odkształceń oraz przemieszczeń konstrukcji nożyc na najbardziej obciążonym pasie autostrady A8. Określono wpływ oddziaływania dynamicznego oraz sporządzono histogramy obciążeń i współczynników przewyższenia dynamicznego na podstawie pomiarów ciągłych w okresie nasilonego ruchu. Wskazano przyczyny degradacji urządzenia. Odniesiono...
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Rozkład naprężeń mechanicznych w łyżce o szerokości 500 mm, przeznaczonej do koparki podsiębiernej
PublicationW artykule zaprezentowano optymalizację kształtu łyżki koparki przedsiębiernej z wykorzystaniem analizy wytrzymałościowej. Analizę oparto na trójwymiarowym modelu konstrukcji łyżki z zastosowaniem metody elementów skończonych (MES). Opracowanie wspomnianej metody pozwoliło na modyfikację konstrukcji łyżki, która dała w efekcie obniżenie naprężeń złożonych w newralgicznych obszarach konstrukcji łyżki. Przedstawiona analiza okazała...
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Badania wytrzymałościowe zgrzein łączących pręty zbrojeniowe o średnicy Ø30mm
PublicationZakres badań dotyczy oceny wytrzymałościowej zgrzein doczołowych, wykonanych metodą zwarciową. Elementami zgrzewanymi były pręty zbrojeniowe o średnicy Ø30mm. Badania związane są z okresową kontrolą stabilności parametrów zgrzewarki, szczególnie w odniesieniu do górnych, dopuszczalnych przez producenta zakresów parametrów zgrzewania oraz analizą wyników i porównaniu rezultatów prób wytrzymałościowych uzyskanych w 2019 i 2020 w...
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Determiantion of optimal conditions for the isolation of oil from the fishing industry offal
PublicationFish processing generates significant amounts of offal, which usually is converted into fodder meal or is stored into landfills. However, some part of this offal could serve as a source of valuable products, such as fish oil, collagen or gelatin. the aim of the study was to determine the conditions of oil isolation from salmon skins ensuring high yield and quality of the product.
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Influence of the internal electrolyte composition on amperometric sulphur dioxide sensor properties
PublicationIn this work investigation of an amperometric sulphur dioxide sensor with a liquid electrolyte, a mercury sulphate reference electrode and solid polymer electrolyte (Nafion) covered with gold as a working electrode properties is presented. The main goal of this research is to determine the influence of internal electrolyte composition on the sensor properties. Sensor filled with 1M and 5M sulphuric acid has been studied.
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Influence of nanoparticle concentration on convective heat transfer of water-Al2O3 nanofluids inside horizontal tubes
PublicationThis work presents preliminary results of the experimental investigation of the forced convection heat transfer of water-Al2O3 nanofluids inside stainless steel tube with 8 mm internal diameter and 2000 mm length. Nanoparticles were tested at the concentrations of 0.01%, 0.1%, 1% and 5% by weight and the Reynolds number range encompasses laminar as well as turbulent flows.
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Iron complexes with terminal and nonbridging phosphanido ligands
PublicationThis review discusses known iron complexes with terminal-bonded and nonbridging phosphanido ligands R2Pand their analogs, such as phosphanylphosphanido, borylphosphanido and oligophosphanido groups, that have been reported since 1960s. Particular attention is focused on the synthesis and structural features of these complexes, which have a direct impact on their philicity and further reactivity. Moreover, their application in catalysis...
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CENTRAL-FORCE DECOMPOSITION OF THE TERSOFF POTENTIAL
PublicationCentral forces play important role in the analysis of results obtained with particle simulation methods, since they allow evaluating stress fields. In this work we derive expressions for a central-force decompositon of the Tersoff potential, which is often used to describe interatomic interactions in covalently bonded materials. We simplify the obtained expressions and discuss their properties.
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The flux compression generator load parameters selection
PublicationComputer simulation results of the flux compression generator (FCG) loaded with an inductor has been presented in this paper. Simulation research has been performed in order to select the parameters of FCG load coil properly. The influence of the load inductance and resistance on the current gain factor and the magnetic field energy accumulated in a load coil has been investigated.
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Propagation in rectangular waveguides with a pseudochiral Ω slab
PublicationThe transfer matrix approach is applied for analysis of waveguides loaded with a uniaxial pseudochiral Ω slab. In particular a pseudochiral parallel plate and rectangular guides are investigated. Based on the numerical analysis the influence of the pseudochirality on propagation characteristics and field distribution are examined. Other feature such as a field displacement phenomenon appearing in the both considered structures...
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Effect of transglutaminase on the solubility of chitosan-gelatin films
PublicationCelem pracy było zmniejszenie rozpuszczalności folii chitozanowo-żelatynowych w środowisku wodnym o różnym pH, poprzez usieciowanie składników przy użyciu transglutaminazy. Niemodyfikowane enzymatycznie folie rozpuszczały się w temperaturze pokojowej w 86 i 64% odpowiednio w buforze o pH 3 i 6. Ich rozpuszczalność została ograniczona do 19-23% po usieciowaniu polimerów enzymem w stężeniu 0.2mg/ml filmotwórczego roztworu. Enzymatycznie...
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Badania eksperymentalne i numeryczne zachowania się zbiorników stalowych poddanych wstrząsom górniczym i sejsmicznym
PublicationW niniejszej dysertacji opisano wyniki kompleksowych badań zachowania się zbiorników stalowych poddanych wstrząsom górniczym i umiarkowanym trzęsieniom ziemi. Badania prowadzono dla dwóch zbiorników rzeczywistych zlokalizowanych na terenie Polski. W pierwszym etapie badań przeprowadzono badania eksperymentalne na specjalnym stanowisku laboratoryjnym dla modeli doświadczalnych zbiorników. Badania prowadzono dla różnego poziomu wypełnienia...
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Wykorzystanie tomografii komputerowej przy analizie jakości zacementowania - migracja gazu
PublicationArtykuł przedstawia opis zjawiska znanego w wiertnictwie otworowym jako migracja gazu. Opisano główne przyczyny powstawania tego negatywnego zjawiska polegającego na przepływie gazów a także płynów w zaczynie cementacyjnym lub kamieniu cementowym. Przygotowano próbki imitujące zacementowany odcinek rur okładzinowych. Skupiono się na wykorzystaniu tomografii komputerowej, oraz analizie zrekonstruowanego trójwymiarowego obrazu, w...
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Wpływ termoplastycznych kompozycji napełnianych miałem gumowym na wybrane właściwości asfaltu drogowego
PublicationDynamiczny rozwój transportu samochodowego zmusza do wykonywania nawierzchni drogowych z materiałów o wysokiej wytrzymałości oraz odporności na odkształcenia trwałe, co znacznie wydłuża żywotność nawierzchni. Polskie przedsiębiorstwo BISEK Sp. z o.o. z Wrocławia we współpracy z Politechniką Wrocławską, jako pierwsze wykorzystało miał gumowy do modyfikacji asfaltów metodą mokrą. W chwili obecnej firma Lotos Asfalt Sp. z o.o. wprowadziła...
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Numerical Simulations and Tracer Studies as a Tool to Support Water Circulation Modeling in Breeding Reservoirs
PublicationThe article presents a proposal of a method for computer-aided design and analysis of breeding reservoirs in zoos and aquariums. The method applied involves the use of computer simulations of water circulation in breeding pools. A mathematical model of a pool was developed, and a tracer study was carried out. A simplified model of two-dimensional flow in the form of a biharmonic equation for the stream function (converted into...
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An in situ technique for the assessment of adhesive properties of a joint under load
PublicationSlow crack propagation in adhesive bonded joints has been characterised using an asymmetric wedge test. Crack position was evaluated from strain gauge measurements, both in the debonded partof the joint and in the bonded zone. Test temperature was changed during loading, giving insight into bond evolution. The technique allows accurate, and virtually continuous, determination of crack position to be made, and therefore the evaluation...
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Preparation of DNA films for studies under vacuum conditions
PublicationExperiments were carried out to determine the optimum conditions required for the preparation of uniform films of supercoiled plasmid DNA to be used in irradiation experiments under high vacuum conditions. Investigations reveal that significant damage to the DNA molecules occurs due to the evacuation process when films were formed from DNA samples in ultra high purity water only. A variety of bases were tested for their possible...
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3D Hierarchical Boron-Doped Diamond-Multilayered Graphene Nanowalls as an Efficient Supercapacitor Electrode
PublicationSynthesis of stable hybrid carbon nanostructure for high-performance supercapacitor electrode with long life-cycle for electronic and energy storage devices is a real challenge. Here, we present a one-step synthesis method to produce conductive boron-doped hybrid carbon nanowalls (HCNWs), where sp2-bonded graphene has been integrated with and over a three-dimensional curved wall-like network of sp3-bonded diamond. The spectroscopic...
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Dissociative Electron Attachment to 5-Iodo-4-thio-2′-deoxyuridine: A Potential Radiosensitizer of Hypoxic Cells
PublicationIn the search for effective radiosensitizers for tumor cells, halogenated uracils have attracted more attention due to their large cross section for dissociation upon the attachment of low-energy electrons. In this study, we investigated dissociative electron attachment (DEA) to 5-iodo-4-thio-2'-deoxyuridine, a potential radiosensitizer using a crossed electron-molecule beam experiment coupled with quadrupole mass spectrometry....
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Investigation of sensing mechanism of Nasicon electrocatalytic sensors in nitrogen dioxide and ammonia
PublicationIn this paper a sensing mechanism of Nasicon electrocatalytic sensor in nitrogen dioxide and ammonia is investigated. Both gases are environmentally hazardous and contain nitrogen atom in the molecule. However, it seems that their sensing mechanism in electrocatalytic sensor could be totally different. Namely, the maximum sensitivity for each gas was obtained at different temperatures. Also, different auxiliary layers are formed...
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Report no: WOiO /II/46/2015 - Construction node verification test, estimation of breaking load (shear force) and destruction form
PublicationA new generation of tanks for LNG transportation has been designed. Mass reduction forced our customer to use three different materials: austenitic steel, aluminium alloy and LNG compressed wood between them. The expertise concerns structural node between tank foundation and suspension. Expertise contains: description of object of test, test principals, results and conclusions
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Two-Stage Identification of Locally Stationary Autoregressive Processes and its Application to the Parametric Spectrum Estimation
PublicationThe problem of identification of a nonstationary autoregressive process with unknown, and possibly time-varying, rate of parameter changes, is considered and solved using the parallel estimation approach. The proposed two-stage estimation scheme, which combines the local estimation approach with the basis function one, offers both quantitative and qualitative improvements compared with the currently used single-stage methods.
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Regularized identification of fast time-varying systems - comparison of two regularization strategies
PublicationThe problem of identification of a time-varying FIR system is considered and solved using the local basis function approach. It is shown that the estimation (tracking) results can be improved by means of regularization. Two variants of regularization are proposed and compared: the classical L2 (ridge) regularization and a new, reweighted L2 one. It is shown that the new approach can outperform the classical one and is computationally...
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PRZE-MIESZKIWANIE [w ramach:] PUSTKA, wystawa zbiorowa
Publication„Prze-mieszkiwanie” to praca ukazująca podstawową potrzebę i konieczność zapewnienia sobie przez wszelkie organizmy żywe, w tym w szczególności przez człowieka, bezpiecznej kryjówki w obliczu dojmującego poczucia postpandemicznej pustki i przymusowej izolacji, ale też kulturowego wykorzenienia. Metaforyczne wyobrażenia archetypicznej formy schronienia pokazuje jej adaptowalność do różnorodnych uwarunkowań, gdzie kontekst stanowi...
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Quantitative proteomic analysis of anticancer drug RH1 resistance in liver carcinoma
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Application of high pressure laser flash photolysis in studies on selected hemoprotein reactions
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Intra- and intersubunit changes accompanying thermal activation of the HtrA2(Omi) protease homotrimer
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The role of the LB structural loop and its interactions with the PDZ domain of the human HtrA3 protease
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Rat cathepsin K: Enzymatic specificity and regulation of its collagenolytic activity
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Reactions of methyl radicals with silica supported silver nanoparticles in aqueous solutions
PublicationSilica supported silver nanoparticles (Ag°–SiO2 –NCs, NCs=nanocomposites) suspended in aqueous solutions are efficient catalysts for the dimerization of methyl radicals to produce ethane, while bare silica is quite inert towards the interaction with methyl radicals. In the presence of small amounts of ethanol adsorbed on the SiO2 surface, the reaction path with methyl radicals is changed and methane is formed as the major product.
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Resonant Frequencies in the Open Microstrip Structures Placed on Curved Surfaces
PublicationThe paper presents the research on open microstrip structures placed on curved surfaces such as cylindrical, elliptical or spherical. The numerical analysis of investigated structures is based on expansion of electric and magnetic fields into suitable function series. Utilizing the continuity conditions the boundary problem is formulated which is solved with the use of method of moments. The investigated structures find application...
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Thermal Decomposition of Carbamoyl Meldrum’s Acids: A Starting Point for the Preparation of 1,3-Oxazine Derivatives
PublicationThe ability to undergo [4 + 2] versus [2 + 2] cycloaddition was under investigation for ketenes thermally generated from carbamoyl Meldrum’s acid. Usually, 1,3-oxazino-5-carbamoylo-4,6-diones are formed when carbamoyl Meldrum’s acid reacts with imine. However, in some cases, a reaction takes an unexpected course, leading to the formation of tetraponerines alkaloids derivatives or cyclic iminoethers.
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Application, design and operation of constructed wetland systems: case studies of systems in the Gdańsk region, Poland
PublicationDifferences in operation result from physical, chemical and biological conditions, which directly influence transformations in the whole aquatic-matrix-plants environment. Depending on the quantity of inflowing organic matter load and the rate of biochemical processes, the contaminations could be removed or/and retuned in the system. From this point of view the facility should be designed and operated in such way that the highest...
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Report no WOiO/II/67/2015 - Expertise about load capacity of twistlock foundation and sliding foundation
PublicationCustomer delivered to Laboratory 17 elements - twist lock foundations and sliding lock foundations (ship equipment for container lashing). Elements were selected from production series. Each element was loaded for braking load. After test visual inspection had been performed. The expertise contains: description of tested elements, test assumptions, test stand, results and conclusions
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Factors influencing decisions to retire – case study of Poland
PublicationThe main objective of the article is to present the factors influencing the decisions of the Poles from the older age groups to withdraw from the labour market by retirement, or on the contrary – to remain economically active, also upon reaching the formal retirement age.
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Partial characterization of three Korean white lotus cultivars
PublicationThe nutritional and bioactive values of Korean white lotus cultivars (Muan, Chungyang, and Garam)extracted with methanol, water, and acetone solvents were evaluated by the contents of their bioactive compounds and antioxidant activity determined by ABTS, CUPRAC, FRAP, DPPH, and β-carotene-linoleic acid assays. HPLC, fluorometry, and FTIR spectroscopy were employed for evaluation of polyphenols and fatty acids. All lotus cultivar...
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Computational methods for calculation of binding free energy for ligand-receptor complexes
PublicationAccurate description of the molecular complexes energetic influence is required for understanding of many biological functions carried out by proteins. Therefore, estimation of binding free energy for ligand-receptor complexes is of highest importance for structure-based ligand design and drug discovery approaches.Experimental methods of determination of difference in Gibbs'es free energy have many limitations. Thus, computational...
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Chiral Pyrazolo[4,3-e][1,2,4]triazine Sulfonamides—Their Biological Activity, Lipophilicity, Protein Affinity, and Metabolic Transformations
PublicationReferring to our previous laboratory results related to the tyrosinase and urease inhibition by pyrazolo[4,3-e][1,2,4]triazine sulfonamides, we examined here in silico the mechanism of action at the molecular level of the investigated pyrazolotriazine sulfonamides by the molecular docking method. The studied compounds being evaluated for their cytotoxic effect against cancer cell lines (MCF-7, K-562) and for recombinant Abl and...
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Effect of convection and microwave heating on the retention of bioactive components in human milk
PublicationBioactive substances are very important components of human milk (HM), especially for premature newborns. The effects of convection (CH) and microwave heating (MWH) at 62.5 and 66 °C, on the level of selected bioactive components of HM: lysozyme (LZ), lactoferrin (LF), secretory immunoglobulin A (sIgA), basal lipase (BL), cytokine TGF-2, vitamin C and total antioxidant capacity (TAC) was compared. Regardless of the used heating methods...
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Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor
PublicationEo-E RNA polymerase transcribes a regulon of folding factors for the bacterial envelope and is induced by physical and chemical stresses. The RseA anti-sigma factor inhibits the activity of Esigma(E) RNA, polymerase. It is shown here that the N-terminal portion of sigma(E), residues 1-153, binds core RNA polymerase. RseA interacts with residues 154-191 of sigma(E), a site that is homologous to region 4, the sigma factor binding...
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X-ray and UV Radiation Damage of dsDNA/Protein Complexes
PublicationRadiation and photodynamic therapies are used for cancer treatment by targeting DNA. However, efficiency is limited due to physico-chemical processes and the insensitivity of native nucleobases to damage. Thus, incorporation of radio- and photosensitizers into these therapies should increase both efficacy and the yield of DNA damage. To date, studies of sensitization processes have been performed on simple model systems, e.g.,...
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Ligand-Modified Boron-Doped Diamond Surface: DFT Insights into the Electronic Properties of Biofunctionalization
PublicationWith the increasing power of computation systems, theoretical calculations provide a means for quick determination of material properties, laying out a research plan, and lowering material development costs. One of the most common is Density Functional Theory (DFT), which allows us to simulate the structure of chemical molecules or crystals and their interaction. In developing a new generation of biosensors, understanding the nature...
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Peptide dendrimers as antifungal agents and carriers for potential antifungal agent—N3‐(4‐methoxyfumaroyl)‐(S)‐2,3‐ diaminopropanoic acid—synthesis and antimicrobial activity
PublicationA series of peptide dendrimers and their conjugates with antimicrobial agent FMDP (N3‐(4‐methoxyfumaroyl)‐(S)‐2,3‐diamino‐propanoic acid) were synthesized. The obtained compounds were tested for the antibacterial and antifungal activity. All novel dendrimers displayed much better activity against the tested strains than FMDP itself. Moreover, their conjugates with FMDP also exhibited antimicrobial activity. The most promising molecules...