Search results for: recombinant Escherichia coli
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Preparation of Ag/Cu-doped titanium (IV) oxide nanoparticles in W/O microemulsion
PublicationNanocząstki Cu-TiO2 oraz Ag/Cu-TiO2 otrzymano w mikroemulsji W/O. Właściwości fotokatalityczne zbadano w procesie degradacji fenolu (0,21mM) w zakresie światła widzialnego. Właściwości biobójcze zbadano poprzez wyznaczenie minimalnego stężenia zahamowania wzrostu (MIC) na szczepach bakterii E. coli, St. aureus oraz grzybów S. Cerevisiae i z rodziny Candida. Dla otrzymanych fotokatalizatorów określono wpływ stężenia domieszki metali...
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Application of safirinium N-hydroxysuccinimide esters to derivatization of peptides for high-resolution mass spectrometry, tandem mass spectrometry, and fluorescent labeling of bacterial cells
PublicationMass spectrometry methods are commonly used in the identification of peptides and biomarkers. Due to a relatively low abundance of proteins in biological samples, there is a need for the development of novel derivatization methods that would improve MS detection limits. Hence, novel fluorescent N-hydroxysuccinimide esters of dihydro-[1,2,4]triazolo[4,3-a]pyridin-2-ium carboxylates (Safirinium P dyes) have been synthesized. The...
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OPUS21 Mechanism of biofilm formation by uropatogenic E. coli producing fimbrial adhesin Dr - studies of proteome and metabolome changes under static and dynamic culture conditions
ProjectsProject realized in Sekcja Finansowa Wydz. Chemiczny according to UMO-2021/41/B/NZ6/03088 agreement from 2021-11-26
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A new B-D-galactosidase with a low temperature optimum isolated from the Antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization.
PublicationA psychrotrophic bacterium producing a coldadaptedB-galactosidase upon growth at low temperatureswas classiWed as Arthrobacter sp. 20B. A genomic DNAlibrary of strain 20B introduced into Escherichia coliTOP10F' and screening on X-Gal (5-bromo-4-chloro-3-indolyl-B-D-galactopyranoside)-containing agar plates ledto the isolation of B-galactosidase gene. The B-galactosidasegene (bgaS) encoding a protein of 1,053 amino acids,with a...
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Optical fiber aptasensor for label-free bacteria detection in small volumes
PublicationHighly sensitive devices for fast bacteria detection are sought to be developed with the task of quantifying the worldwide problem of pathogenic bacteria and thus helping to take control over spreading bacterial infections. This work concerns a sensing solution based on microcavity in-line Mach-Zehnder interferometer (μIMZI) induced in an optical fiber. Such a device exhibits ultrahigh sensitivity to refractive index changes...
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Targeting of FLT3-ITD kinase contributes to high selectivity of imidazoacridinone C-1311 against FLT3-activated leukemia cells
PublicationDrugs targeting receptor tyrosine kinase FLT3 are of particular interest since activating FLT3-internal tandem duplication (ITD) mutations abundantly occur in fatal acute myeloid leukemias (AMLs). Imidazoacridinone C-1311, a DNA-reactive inhibitor of topoisomerase II, has been previously shown to be a potent and selective inhibitor of recombinant FLT3. Here, we expand those findings by studying its effect on leukemia cells with...
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Composite Polyurethane-Polylactide (PUR/PLA) Flexible Filaments for 3D Fused Filament Fabrication (FFF) of Antibacterial Wound Dressings for Skin Regeneration
Publicationhis paper addresses the potential application of flexible thermoplastic polyurethane (TPU) and poly(lactic acid) (PLA) compositions as a material for the production of antibacterial wound dressings using the Fused Filament Fabrication (FFF) 3D printing method. On the market, there are medical-grade polyurethane filaments available, but few of them have properties required for the fabrication of wound dressings, such as flexibility...
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Quantification of unconjugated and total ibuprofen and its metabolites in equine urine samples by gas chromatography–tandem mass spectrometry: Application to the excretion study
PublicationIbuprofen (IBU) is a widely used nonsteroidal anti-inflammatory drug (NSAID) in human and also veterinary medicine. NSAIDs are usually highly metabolized compounds in horses; they are often present in equine urine, mostly in their conjugated forms (glucuronides). Thus, hydrolysis to cleave the glucuronide linkage prior to anti-doping analysis is often necessary for improving detection. In this study, unconjugated and total IBU...
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The Anti-Staphylococcal Potential of Ethanolic Polish Propolis Extracts
PublicationAbstract: The principal objective of this study was to determine the anti-staphylococcal potential of ethanol extracts of propolis (EEPs). A total of 20 samples of propolis collected from apiaries located in different regions of Poland were used in the study. The two-fold broth microdilution method revealed some important differences in the antimicrobial activity of investigated EEPs. Up to the concentration of 4096 µg/mL no activity...
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Thermosensitive composite based on agarose and chitosan saturated with carbon dioxide. Preliminary study of requirements for production of new CSAG bioink.
PublicationThis study introduces a method for producing printable, thermosensitive bioink formulated from agarose (AG) and carbon dioxide-saturated chitosan (CS) hydrogels. The research identified medium molecular weight chitosan as optimal for bioink production, with a preferred chitosan hydrogel content of 40–60 %. Rheological analysis reveals the bioink's pseudoplastic behavior and a sol-gel phase transition between 27.0 and 31.5 °C. The...
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Paenibacillus alvei MP1 as a Producer of the Proteinaceous Compound with Activity against Important Human Pathogens, Including Staphylococcus aureus and Listeria monocytogenes
PublicationAn emerging need for new classes of antibiotics is, on the one hand, evident as antimicrobial resistance continues to rise. On the other hand, the awareness of the pros and cons of chemically synthesized compounds’ extensive use leads to a search for new metabolites in already known reservoirs. Previous research showed that Paenibacillus strain (P. alvei MP1) recovered from a buckwheat honey sample presented a wide spectrum of...
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The Synergistic Microbiological Effects of Industrial Produced Packaging Polyethylene Films Incorporated with Zinc Nanoparticles
PublicationZinc compounds in polyolefin films regulate the transmission of UV-VIS radiation, affect mechanical properties and antimicrobial activity. According to hypothesis, the use of zinc- containing masterbatches in polyethylene films (PE) with different chemical nature—hydrophilic zinc oxide (ZO) and hydrophobic zinc stearate (ZS)—can cause a synergistic effect, especially due to their antimicrobial properties. PE films obtained on an...
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Identification and cloning of C. albicans SC5314 genes encoding L-methionine biosynthetic pathway enzymes.
Open Research DataEnzymes of fungal L-methionine biosynthetic pathway: homoserine O-acetyltransferase (Met2p), O-acetylhomoserine sulfhydrylase (Met15p) and cystathionine-γ-synthase (Str2p) could be exploited as molecular targets for antifungal chemotherapy. The goal of the study was to identify and clone genes encoding mentioned above enzymes. MET2, MET15 and STR2 genes...
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Peptidoglycan hydrolases - potential weapons against Staphylococcus aureus
PublicationBacteria of the genus Staphylococcus are common pathogens responsible for a broad spectrum of human and animal infections and belong to most important etiological factors causing food poisoning. Because of rapid increase in prevalence of isolation of staphylococci resistant to many antibiotics, there is an urgent need for development of new alternative chemotherapeutics. A number of studies have recently demonstrated the strong...
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Jakość wody w małych wiejskich wodociągach w województwie pomorskim w latach 2000 – 2004
PublicationW publikacji poddano ocenie jakość wody w małych wiejskich wodociągach bezpośrednio przed przystąpieniem Polski do Unii Europejskiej. Analizie poddano 79 losowo wybranych wiejskich wodociągów publicznych województwa pomorskiego, z około 740 tego typu obiektów w województwie. Zdecydowana większość poddanych analizie wodociągów (ok. 90%) zaopatrywała do 1500 mieszkańców a produkcja wody nie przekraczała 350 m3/dobę. Jedynie w około...
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Increased concentration of Taq DNA polymerase as a solution for GC-rich templates from clinical and environmental samples
PublicationDNA polymerase is an enzyme which plays crucial role in replication and DNA repair. It found application in PCR (polymerase chain reaction) where catalyses process of in vitro DNA synthesis. To meet the demands posed by mod- ern diagnostic, molecular biology or genetic engineering it is necessary to improve DNA polymerases to obtain new or better features useful in these fields. So far implemented modifications in majority are...
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The influence of electromagnetic pollution on living organisms – historical trends and forecasting changes
PublicationCurrent technologies have become a source of omnipresent electromagnetic pollution from generated electromagnetic fields and resulting electromagnetic radiation. In many cases this pollution is much stronger than any natural sources of electromagnetic fields or radiation. Wireless and radio communication, electric power transmission or devices in daily use such as smartphones, tablets and portable computers every day expose people...
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Identyfikacja i izolacja genu kodującego zaadaptowaną do zimna β-galaktozydazę Paracoccus sp. = Identification and izolation of the gene encoding cold-active β-galactosidase from paracoccus sp.
PublicationZaprezentowane badania były prowadzone u eehi wyizolowania ze środo¬wiska organizmu psychrofilnego, zdolnego do hydrolizy laktozy w niskiej tempe¬raturze, oraz zidentyfikowania genu kodującego (i-galaktozydazę zaadaptowaną do zimna.Poszukiwan gen został w izolowany z psychrofilnego szczepu autarkicz¬nego Paracoccus sp. 321). Na podstawie sekwencji nukleotydowej genu kodują¬cego fS-galaktozdaze ustalono skład aminokwasów),...
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Synthesis, Characterization and Biological Investigations of Half-Sandwich Ruthenium(II) Complexes Containing Benzimidazole Moiety
PublicationHalf-sandwich Ru(II) complexes belong to group of biologically active metallo-compounds with promising antimicrobial and anticancer activity. Herein, we report the synthesis and characteri- zation of arene ruthenium complexes containing benzimidazole moiety, namely, [(η6-p-cymene)RuC l(bimCOO)] (1) and [(η6-p-cymene)RuCl2(bim)] (2) (where bimCOO = benzimidazole-2-carboxylate and bim = 1-H-benzimidazole). The compounds were characterized...
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Laboratoryjne badania nad możliwością skażenia gruntów i wód podziemnych jako rezultatu stosowania osadów ściekowych
PublicationW pracy przedstawiono cenę zagrożeń związanych z możliwością skażenia gruntów i płytkich wód gruntowych w wyniku stosowania osadów ściekowych na mineralnych gruntach piaszczystych i na popiołach lotnych, o charakterystykach zbliżonych do niektórych terenów zdegradowanych technicznie. Uzyskane wyniki pozwoliły na prześledzenie dynamiki wymywania żywych komórek bakteryjnych z osadu ściekowego oraz ich dalszej retencji w materiale...
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Novel Resveratrol-Based Substrates for Human Hepatic, Renal, and Intestinal UDP-Glucuronosyltransferases
PublicationTrans-Resveratrol (tRes) has been shown to have powerful antioxidant, anti-inflammatory, anti-carcinogenic, and anti-aging properties; however, its use as a therapeutic agent is limited by its rapid metabolism into its conjugated forms by UDP-Glucuronosyltransferases (UGTs). The aim of the current study was to test the hypothesis that the limited bioavailability of tRes can be improved by modifying its structure to create analogs...
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Enzymes of the lysine biosynthetic pathway as targets for antifungals ?
PublicationSystemic infections caused by human pathogenic fungi in immunocompromized patients continue to be one of the important clinical problems. Limited availability of safe and efficacious antifungal chemotherapeutics and emerging resistance to existing drugs stimulates search for novel molecular targets for antifungals. The α-aminoadipate pathway (AAP) of L-lysine biosynthesis is unique in fungi and thus has been so far considered...
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Discrimination of hospital isolates of Acinetobacter baumannii using repeated sequences and whole genome alignment differential analysis
PublicationAn optimized method for bacterial strain differentiation, based on combination of Repeated Sequences and Whole Genome Alignment Differential Analysis (RS&WGADA), is presented in this report. In this analysis, 51 Acinetobacter baumannii multidrug-resistance strains from one hospital environment and patients from 14 hospital wards were classified on the basis of polymorphisms of repeated sequences located in CRISPR region, variation...
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PVA-Based Films with Strontium Titanate Nanoparticles Dedicated to Wound Dressing Application
PublicationBioactive materials may be applied in tissue regeneration, and an example of such materials are wound dressings, which are used to accelerate skin healing, especially after trauma. Here, we proposed a novel dressing enriched by a bioactive component. The aim of our study was to prepare and characterize poly(vinyl alcohol) films modified with strontium titanate nanoparticles. The physicochemical properties of films were studied,...
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Successive cytotoxicity control by evolutionary surface decorated electronic push-pull green ZnCr-LDH nanostructures: Drug delivery enlargement for targeted breast cancer chemotherapy
PublicationThe reason for the increasing bioavailability and biocompatibility of the porous nanomaterials in the presence of different (bio)molecules is still unknown. The role of difference functional groups and their interactions with the potential bioavailability and biocompatibility is of great importance. To investigate the potential contribution of the electronic effects (especially on the surface of the porous nanomaterials) on their...
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Shear stress connected with the fluid flow as an important parameter influencing the adhesion, invasion and tissue tropism of the uropathogenic E. coli polyadhesins to the host cells.
ProjectsProject realized in Department of Microbiology according to UMO-2014/13/B/NZ6/00257 agreement from 2015-02-09
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STORMWATER AS AN ALTERNATIVE WATER SOURCE: QUALITY CHANGES WITH RAINFALL DURATION AND IMPLICATIONS FOR TREATMENT APPROACHES
PublicationThe pressure on the world’s water resources is rapidly increasing due to population growth and climatic changes. Valorisation of stormwater as a water resource for non-potable reuse can reduce high-quality water demands and save it for potable uses. In this context, not only roof runoff but also drained stormwater outflow, representing considerably higher pollution levels, should be considered as a potential resource. We analysed...
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Osteoblast and bacterial cell response on RGD peptide‐functionalized chitosan coatings electrophoretically deposited from different suspensions on Ti13Nb13Zr alloy
PublicationMetallic materials for long-term load-bearing implants still do not provide high antimicrobial activity while maintaining strong compatibility with bone cells. This study aimed to modify the surface of Ti13Nb13Zr alloy by electrophoretic deposition of a chitosan coating with a covalently attached Arg-Gly-Asp (RGD) peptide. The suspensions for coating deposition were prepared in two different ways either using hydroxyacetic acid...
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Cytocompatibility, antibacterial, and corrosion properties of chitosan/polymethacrylates and chitosan/poly(4‐vinylpyridine) smart coatings, electrophoretically deposited on nanosilver‐decorated titania nanotubes
PublicationThe development of novel implants subjected to surface modification to achieve high osteointegration properties at simultaneous antimicrobial activity is a highly current problem. This study involved different surface treatments of titanium surface, mainly by electrochemical oxidation to produce a nanotubular oxide layer (TNTs), a subsequent electrochemical reduction of silver nitrate and decoration of a nanotubular surface with...
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Characterization of two aminotransferases from Candida albicans
PublicationAminoadipate aminotransferase (AmAA) is an enzyme of α-aminoadipate pathway (AAP) for l-lysine biosynthesis. AmAA may also participated in biosynthesis or degradation of aromatic amino acids and in d-tryptophan based pigment production. The AAP is unique for fungal microorganisms. Enzymes involved in this pathway have specific structures and properties. These features can be used as potential molecular markers. Enzymes catalyzing...
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A cold-adapted esterase from psychrotrophic Pseudoalteromonas sp. strain 643A
PublicationDzięki zastosowaniu lipaz możliwa jest biotechnologiczna produkcja m.in. biodegradowalnych biopolimerów, biopaliw (tzw. biodiesel), wielu terapeutyków, herbicydów, pestycydów, kosmetyków, substancji smakowych, serów i in. Szczególnie interesującą grupę stanowią enzymy lipolityczne, pochodzące z organizmów psychrofilnych i psychrotrofowych, ze względu na zdolność do wydajnego katalizowania reakcji w temperaturach rzędu 10-20 ºC....
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Glucosamine-6-phosphate synthase with an oligoHis insert
PublicationGlucosamine-6-phosphate (GlcN-6-P) synthase known also as L-Glutamine: D-fructose-6-phosphate aminotransferase (EC 2.6.1.16), catalyzes the first committed step in the amino sugar biosynthetic pathway in prokaryotic and eukaryotic organisms. The final product of this pathway is an activated precursor of numerous macromolecules containing amino sugars, including chitin and mannproteins in fungi, peptydoglican and lipopolysaccharides...
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Structural features responsible for control of the eukaryotic GlcN-6-P synthase activity
PublicationGlucosamine-6-phosphate (GlcN-6-P) synthase is responsible for catalysis of the first and practically irreversible step in hexosamine metabolism. As UDP-GlcNAc, the final product of the hexosamine path, constitutes one of the essential substrates for assembly of bacterial and fungal cell walls, the enzyme is an interesting target for antimicrobial therapy.The structure of E. coli enzyme, known since 2001, forms a dimer of two identical...
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Ultrafiltration Process in Disinfection and Advanced Treatment of Tertiary Treated Wastewater
PublicationThe paper presents the results of research on the use of ultrafiltration, using membranes of 200 and 400 kDa separation, for disinfection of municipal treated wastewater. The research was conducted on a fractional technical scale using real municipal treated wastewater from two large wastewater treatment plants treating most of the wastewater over the one-million polycentric Gdańsk agglomeration (1.2 million inhabitants). UF 200...
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Przydatność wybranych kruszyw mineralnych do sorpcji fosforu podczas doczyszczania ścieków bytowych
PublicationBadania przeprowadzono na specjalnym stanowisku z trzema parami kolumn wypełnionych złożami filtracyjnymi poszczególnych materiałów, stosując dla każdego z nich po dwa nominalne obciążenia hydrauliczne: 20 i 40 dm3/d. W trakcie badań wykluczono z dalszych prób materiał nr 2, stwierdzając jego nieprzydatność do praktycznego wykorzystania, ze względu na jego intensywne ługowanie i wypłukiwanie, skutkujące narastającą kolmatacją złóż...
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Określenie zawartości lotnych kwasów tłuszczowych (LKT) w odciekach pochodzących z mikrobiologicznej degradacji monosacharydów
PublicationOkreślenie zawartości lotnych kwasów tłuszczowych (LKT) w odciekach pochodzących z mikrobiologicznej degradacji monosacharydów. Milena Jabłońska, Maria Rybarczyk, Marta Wisielewska, Marek Lieder Politechnika Gdańska, Wydział Chemiczny, Katedra Technologii Chemicznej Zawartość lotnych kwasów tłuszczowych (LKT) w metabolitach pochodzących z mikrobiologicznego ogniwa paliwowego (MFC – Microbial Fuel Cell) świadczy o rozkładzie...