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A vector-enzymatic DNA fragment amplification-expression technology for construction of artificial, concatemeric DNA, RNA and proteins for novel biomaterials, biomedical and industrial applications
PublicationA DNA fragment amplification/expression technology for the production of new generation biomaterials for scientific, industrial and biomedical applications is described. The technology enables the formation of artificial Open Reading Frames (ORFs) encoding concatemeric RNAs and proteins. It recruits the Type IIS SapI restriction endonuclease (REase) for an assembling of DNA fragments in an ordered head-to-tail-orientation. The...
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A GPU Solver for Sparse Generalized Eigenvalue Problems with Symmetric Complex-Valued Matrices Obtained Using Higher-Order FEM
PublicationThe paper discusses a fast implementation of the stabilized locally optimal block preconditioned conjugate gradient (sLOBPCG) method, using a hierarchical multilevel preconditioner to solve nonHermitian sparse generalized eigenvalue problems with large symmetric complex-valued matrices obtained using the higher-order finite-element method (FEM), applied to the analysis of a microwave resonator. The resonant frequencies of the low-order...
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Vernacular and neovernacular architecture without a user. Wooden catholic churches in the vicinity of brody in Ukraine.
PublicationOne of the basic theoretical postulates for the protection of monuments is to preserve them in the place of their creation. However, such action is not always possible to implement. The great historical processes of the 20th century led to significant national, political, religious and cultural changes in the border areas between Poland and Ukraine. As a result of those changes, a large number of wooden churches have lost their...
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Certified domination
PublicationImagine that we are given a set D of officials and a set W of civils. For each civil x ∈ W, there must be an official v ∈ D that can serve x, and whenever any such v is serving x, there must also be another civil w ∈ W that observes v, that is, w may act as a kind of witness, to avoid any abuse from v. What is the minimum number of officials to guarantee such a service, assuming a given social network? In this paper, we introduce...
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Elemental and water-insoluble organic carbon in Svalbard snow: a synthesis of observations during 2007–2018
PublicationLight-absorbing carbonaceous aerosols emitted by biomass or fossil fuel combustion can contribute to amplifying Arctic climate warming by lowering the albedo of snow. The Svalbard archipelago, being near to Europe and Russia, is particularly affected by these pollutants, and improved knowledge of their distribution in snow is needed to assess their impact. Here we present and synthesize new data obtained on Svalbard between 2007...
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The extended version of restriction analysis approach for the examination of the ability of low-molecular-weight compounds to modify DNA in a cell-free system
PublicationOne of the primary requirements in toxicology is the assessment of ability of chemicals to induce DNA covalent modification. There are several well-established methods used for this purpose such as 32P-Postlabeling or HPLC-MS. However, all of these approaches have difficult to overcome limitations, which prevents their use in genotoxin screening. Here, we describe the simple protocol exploiting specificity of restriction enzymes...
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Specific detection of Alternaria alternata by PCR and real-time PCR
PublicationFungi of Alternaria genus are cosmopolitan organisms, which spores can be found in the air, soil, water, clothing and food. They commonly occur as saprotrophs on the plant remains, contributing to the decomposition of organic matter. Additionally, they are components of the normal human and animal skin flora. Alternaria spp. are also known human allergens, causing hay fever and allergic reactions that can lead to the development...
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Applying ground penetrating radar to tracking of ancient architectural transformations: the case of the monastery St. Peter on the Island of Rab (Croatia)
PublicationThe ground-penetrating radar (GPR) method has been used for many years in archaeological research. However, thismethod is still not widely used in studies of past architecture. The biggest problem with the implementation of the GPRmethod at such sites is usually connected with extensive debris layers, plant cover and standing relics of walls and otherfeatures that restrict the available measurement area. Despite of these, properly...
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H2AX phosphorylation, its role in DNA damage response and cancer therapy
PublicationDouble-strand breaks (DSBs) are the most deleterious DNA lesions, which, if left unrepaired, may have severe consequences for cell survival, as they lead to chromosome aberrations, genomic instability, or cell death. Various physical, chemical, and biological factors are involved in DSB induction. Cells respond to DNA damage by activating the so-called DNA damage response (DDR), a complex molecular mechanism developed to detect...
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Evaluation of organic coatings condition with AFM-based method
PublicationThe paper presents an atomic force microscopy (AFM)-based approach to evaluation of local protective properties of organic coatings. Apart from topography, it provides local ac and dc characteristics of examined coating. The method consists in application of ac voltage perturbation signal between conductive AFM tip and coated metal substrate. The resulting current is used to determine local impedance characteristics. Both impedance...
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Determination of polycyclic aromatic hydrocarbons (PAHs) and other organic pollutants in freshwaters on the western shore of Admiralty Bay (King George Island, Maritime Antarctica).
PublicationOrganic contamination in freshwater samples has never been investigated at the western shore of Admiralty Bay. Therefore, the presence of polycyclic aromatic hydrocarbons (PAHs) in five different sites distributed along a shore running from the Arctowski Station to the Baranowski Glacier was studied. Moreover, organic compounds such as n-alkanes, toluene and ethylbenzene were also noted. Increased ΣPAHs in late Austral summer 2016...
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COMPARATIVE ANALYSIS OF POINT CLOUDS OBTAINED BY TLS AND PHOTOGRAMMETRY
PublicationTechnical progress in the field of 3D modelling resulted in increasing use of such technics to create a virtual 3D models of objects. Three-dimensional spatial models are used in a wide range of applications such as inventorisation of architectural-construction, an inventorisation of archaeological sites, medicine and wide-known engineering. Spatial models are created based on the point clouds acquired with laser scanners or photogrammetry....
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Low-Dimensional Magnetic Semimetal Cr0.65Al1.35Se3
PublicationWhile exploring novel magnetic semiconductors, the new phase Cr0.65Al1.35Se3 was discovered and characterized by both structural and physical properties. Cr0.65Al1.35Se3 was found to crystallize into orthorhombic CrGeTe3-type structure with space group Pnma (no. 62). Vacancies and mixed occupancies were tested, and the results show that one of the 4c sites accommodates a mixture of Cr and Al atoms, while the other 4c site is fully...
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Point cloud in archaeological and historical survey
PublicationIn the last decade the potential of using the point clouds in archaeological research has been noted. Therefore, a number of guidelines concerning aspects of data acquisition (resolution and format) were developed. They also included the way of searching the imaged area to identify archaeological and historical cultural heritage sites. The purpose of this research was to analyze the point cloud for selected woodland survey. Forest...
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Numerical Analysis of an Impact of Planned Location of Sewage Discharge on Natura 2000 Areas – The Dead Vistula Region Case Study
PublicationThis article presents results of an analysis of impact of a designed discharge of contaminated water into the Dead Vistula (Wisła Martwa) in the region of the Isthmus (Przesmyk) with the aim of determination of a possible effect of the pollution onto protected areas of Natura 2000 (bird habitats and sites, especially the Bird Paradise – Ptasi Raj) nature reserve. The analysis was conducted on the basis of the two-dimensional modelling...
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Hierarchical MnO2 nanoflowers blooming on 3D nickel foam: A novel micro-macro catalyst for peroxymonosulfate activation
PublicationIn this work, birnessite-type δ-MnO2 nanoflowers were uniformly deposited on 3D nickel foam (NF) by one-step hydrothermal route for high-efficient activation of peroxymonosulfate (PMS) towards degradation of acid orange 7 (AO7). High specific surface area, large pore volume and 3D hierarchical structure promotes the mass and electron transfer for great catalytic activity. Low reaction energy barrier (Ea=27.5 kJ/mol) and outstanding...
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Activities of the Astro-geodetic Observatory in Jozefoslaw in the frame of IGS and EUREF
PublicationThe Astrogeodetic Observatory of the Institute of Geodesy and Geodetic Astronomy of the Warsaw University of Technology (WUT) in Jozefoslaw was incorporated in 1957. In 1991 it joined the International GPS Service (IGS) and started to operate as a permanent in 1993. Since then the following permanent services have been maintained: GPS observations using Trimble and Turbo Rogue receivers, tidal gravimetric observations with LaCoste&Romberg...
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Chromatographic analyses of Lavandula angustifolia and Rosmarinus officinalis extracts and their biological effects in mammalian cells and cell-free systems
PublicationKnowledge of biological properties of natural compounds allows to understand their therapeutic value, efficacy and security. We investigated: composition of Lavandula angustifolia (LA) and Rosmarinus officinalis (RO) extracts, their antioxidant capacity, cytotoxicity and genotoxicity, their DNA-protective potential against DNA damage induced in hamster V79 cells by several genotoxins or in plasmid DNA by Fe2+ ions and activity...
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Full scale CFD seakeeping simulations for case study ship redesigned from V-shaped bulbous bow to X-bow hull form
PublicationIncreasing propulsion efficiency, safety, comfort and operability are of the great importance, especially for small ships operating on windy sites like the North Sea and the Baltic Sea. Seakeeping performance of ships and offshore structures can be analysed by different methods and the one that is becoming increasingly important is CFD RANS. The recent development of simulation techniques together with rising HPC accessibility...
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Dzielnica Reprezentacyjna Gdyni i jej powojenne oraz współczesne interpretacje
PublicationThe concept of the Premiere District in Gdynia was first conceived in the 1920s and from that time on it constantly accompanied the history of the city. It derivated from the idea of creating a symbol of Poland's regaining independence after the first World War and the successful creation of the new port and city on the Baltic coast. As the result of that development, Gdynia was named the maritime capital of Poland and was treated...
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Directions and Prospects for the Development of the Electric Car Market in Selected ASEAN Countries
PublicationThe purpose of this article is to present the current situation and evaluate the opportuni‐ ties for the development of the electric car market in selected Southeast Asian countries in the con‐ text of the current situation in the rest of the world. Currently, the electric car market is at an ad‐ vanced stage of development in regions such as Western Europe, the USA, and China. It should be noted, however, that the number of electric...
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Wybrane aspekty zachowań internautów oraz rozwój komunikacji marketingowej w Internecie - Polska na tle innych krajów europejskich
PublicationCelem artykułu jest zaprezentowanie różnic w zachowaniach klientów on-line oraz rozwoju komunikacji marketingowej w Internecie. W opracowaniu powołano się na wyniki badań internautów przeprowadzonych w Polsce i w innych krajach Europy. Przybliżono w nim najbardziej charakterystyczne cechy i zachowania użytkowników Internetu. Oprócz tego, w artykule ukazano istotę podejścia inbound marketing w kreowaniu wartościowych treści z punktu...
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Identification of acoustic event of selected noise sources in a long-term environmental monitoring systems
PublicationABSTRACT Undertaking long-term acoustic measurements on sites located near an airport is related to a problem of large quantities of recorded data, which very often represents information not related to flight operations. In such areas, usually defined as zone of limited use, often other sources of noise exist, such as roads or railway lines treated is such context as acoustic background. Manual verification of such recorded data...
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Representativity of ISO test track surface based on controlled pass-by measurements
PublicationWithin the Polish-Norwegian research project ELANORE, a limited Round Robin Test has been performed on four ISO tracks in Northern Europe. The basic objective of the project is to improve the EU directive on labelling of tyres for noise and rolling resistance. This directive is based on the measurement procedures proscribed in the UNECE Reg.117. Measurements on 3 ISO tracks were performed in 2021 and presented at InterNoise 2022....
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Oxygen vacancy-enriched V2O5·nH2O nanofibers ink for universal substrates-tolerant and multi means-integratable NH3 sensing
PublicationUniversal substrates-tolerant and multi means-integratable ammonia (NH3) sensing is highly desired in future Internet of Things in environmental monitoring, food security and early diagnosis of human diseases, however, is still less than satisfactory. Here, an oxygen vacancy-governed NH3 sensing has been developed with V2O5·nH2O nanofibers (NFs) ink, via combined thermal decomposition of ammonium metavanadate and dilution. As-obtained...
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Multi-Taper-Based Automatic Correction of Non-Anechoic Antenna Measurements
PublicationPrototype measurements belong to the key steps in the development of antenna structures. Although accurate validation of their far-field performance can be realized in dedicated facilities, such as anechoic chambers, the high cost of their construction and maintenance might not be justified if the main goal of measurements is to support teaching or low-budget research. Instead, they can be performed in non-anechoic conditions and...
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Analysis of GNSS, Hydroacoustic and Optoelectronic Data Integration Methods Used in Hydrography
PublicationThe integration of geospatial data in hydrography, performed using different measurement systems, involves combining several study results to provide a comprehensive analysis. Each of the hydroacoustic and optoelectronic systems is characterised by a different spatial reference system and the method for technical implementation of the measurement. Therefore, the integration of hydrographic data requires that problems in selected...
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Atmospheric degradation mechanism of anthracene initiated by OH•: A DFT prediction
PublicationDensity functional theory (DFT) calculations at the M06-2X/def2-TZVP level have been employed to investigate the atmospheric oxidation mechanism of anthracene (ANT) initiated by HO•. Direct hydrogen atom abstraction from the ANT using HO• takes place hardly at ambient conditions while the addition of HO• to the C1, C2, and C4 sites are thermodynamically and kinetically more advantageous. The addition reactions are controlled by...
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Dimeric structure of the N-terminal domain of PriB protein from Thermoanaerobacter tengcongensis solved ab initio
PublicationPriB is one of the components of the bacterial primosome, which catalyzes the reactivation of stalled replication forks at sites of DNA damage. The N-terminal domain of the PriB protein from the thermophilic bacterium Thermoanaerobacter tengcongensis (TtePriB) was expressed and its crystal structure was solved at the atomic resolution of 1.09 Å by direct methods. The protein chain, which encompasses the first 104 residues of the...
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Enhanced boron doping of thin diamond films grown in deuterium-rich microwave plasma
PublicationThe boron-doped diamond thin films were growth in deuterium rich microwave plasma in CVD process. The mechanism of influence of plasma composition on boron doping level was studied using optical emission spectroscopy. Deuterium rich plasma results in an increased dissociation of B2H6 precursor and intense boron-radicals' production. In consequence, a higher doping level of diamond films was observed by means of Laser Induced Breakdown...
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A rapid-response ultrasensitive biosensor for influenza virus detection using antibody modified boron-doped diamond
PublicationAccording to the World Health Organization (WHO), almost 2 billion people each year are infected worldwide with flu-like pathogens including influenza. This is a contagious disease caused by viruses belonging to the family Orthomyxoviridae. Employee absenteeism caused by flu infection costs hundreds of millions of dollars every year. To successfully treat influenza virus infections, detection of the virus during the initial development...
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Study of pH and temperature effect on lipophilicity of catechol-containing antioxidants by reversed phase liquid chromatography
PublicationLipophilicity of selected antioxidant phytochemicals, including flavonoids, phenolic acids and xanthonoids, was determined by reversed phase high performance liquid chromatography with UV detection (RP-HPLC-UV). The analyses run at different temperature and pH conditions in isocratic mode suggested that lipophilicity as distribution coefficient (logD) between aqueous and organic phase decreases with increasing temperature. For...
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Tworzenie miasta przyjaznego wszystkim
PublicationTworzenie miasta przyjaznego wszystkim, to zadnie, które jest prawdopodobnie celem wszystkich włodarzy miejskich. Czy nie jest tak, że każdy chciałby w takim mieście zamiesz-kać? Jednak zadanie to jest dość trudne, bo wymaga pogodzenia wielu aspektów życia mieszkańców. Obecnie do zagadnień środowiskowych związanych z szeroko rozumianą ekologią dochodzi zagadnienie dostosowania przestrzeni zgodnie z zasadami projektowania uniwersalnego....
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Rekonstrukcja ewolucji układu przestrzennego średniowiecznego miasta i portu Szczecin Reconstruction of the Spatial Evolution of the Medieval City and Port of Szczecin
PublicationPraca rekonstruuje ewolucję układu osadniczego Szczecina w okresie od połowy VIII w. do początku XIV w., w oparciu o interpretację dostępnej literatury naukowej dotyczącej historii i archeologii Szczecina oraz metodę analizy planistycznej. Bardzo istotnym elementem opisu struktury urbanistycznej wczesnośredniowiecznego Szczecina było określenie roli i położenia portu. W wyniku analizy zmian układu przestrzennego miasta w omawianym...
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URBAN MENTORING JAKO NOWA TECHNIKA WPRÓŁPRACY W PROCESIE PLANOWANIA PARTCYPACYJNEGO
PublicationW 25 lat od powrotu demokracji opierającej się o neoliberalny paradygmat rozwoju kraju, Polska przyjęła Krajową Politykę Miejską 2030. Wraz z ustawą o rewitalizacji w nowoczesny sposób określa ona pole współpracy władz samorządowych i administracji z mieszkańcami, przejście do drugiej fazy rozwoju społeczeństwa demokratycznego – mieszkańców współgospodarzy przestrzeni miast. Powolna ewolucja tej relacji, silnie obarczonej dziedzictwem...
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Sonocatalytic degradation of Bisphenol A from aquatic matrices over Pd/CeO2 nanoparticles: Kinetics study, transformation products, and toxicity
PublicationIn this work, different ratios of palladium – cerium oxide (Pd/CeO2) catalyst were synthesized and characterized, while their sonocatalytic activity was evaluated for the degradation of the xenobiotic Bisphenol A (BPA) from aqueous solutions. Sonocatalytic activity expressed as BPA decomposition exhibited a volcano-type behavior in relation to the Pd loading, and the 0.25Pd/CeO2 catalyst characterized by the maximum Pd dispersion...
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Review of Selected Methods for Prediction of Added Resistance in Following Waves
PublicationThe added resistance in waves is a mean value of non-linear, second order reaction of a ship to incoming waves. In the beginning of the 20th century, the experimental methods for investigation of ship hydrodynamics at model scale were developed. They allowed the evaluation of added resistance by measurements in irregular waves (directly) or by measurements in regular waves (in-direct method). The main goal was to find more precise...
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Diatomaceous earth as a drug-loaded carrier in a glass-ionomer cement
PublicationThe effect of a natural filler (diatomaceous earth [DE], a promising drug-delivery agent) and its content was investigated on the performance of a model glass-ionomer cement (GIC). Three sample series, differing in DE content (0, 2.5 and 5 wt%), were prepared using a commercial GIC as a matrix (3M Ketac Molar Easymix). The resultant surface microhardness and roughness, wear performance, and compressive strength of the samples were...
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Organizacja systemu opieki nad seniorami wyzwaniem dla samorządów lokalnych
PublicationA significant part of the activities undertaken so far under the senior policy, also at the local level, have been focused on seniors’ social (physical and educational) activation. The growing number of the oldest seniors, with limited independence, living alone in single‑person households, away from their families, will force the policy priorities to be reoriented in such a way as to develop efforts to provide different types...
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Effect of Arc Strikes on High Strength Low Alloy Steels Welded by SMAW
PublicationWet welding with covered electrodes (Shielded Metal Arc Welding – SMAW) is the most commonly used method of carrying out welding repair works in a water environment. Limited visibility and the inability to move freely under water result in an increased risk of formation of welding imperfections such as lack of fusion, lack of penetration and arc strikes. The work focused on changes in the properties and structure of steel subjected...
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Application of Text Analytics in Public Service Co-Creation: Literature Review and Research Framework
PublicationThe public sector faces several challenges, such as a number of external and internal demands for change, citizens' dissatisfaction and frustration with public sector organizations, that need to be addressed. An alternative to the traditional top-down development of public services is co-creation of public services. Co-creation promotes collaboration between stakeholders with the aim to create better public services and achieve...
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An attempt to create a digital reconstruction of the Copper Ship = Próba cyfrowej rekonstrukcji kadłuba wraku Miedziowca
PublicationThis study presents an attempt to create a digital reconstruction of the W-5 shipwreck (the Copper Ship) based on data acquired by 3D scanning of structural components held at the National Maritime Museum in Gdańsk and on a physical reconstruction model of the ship’s hull. A digital reconstruction would facilitate analysis of various possible options for the structural design of the hull, and would enable the preparation of a model for...
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Stormwater and snowmelt runoff storage control and flash flood hazard forecasting in the urbanized coastal basin.
PublicationCity of Gdańsk is located in a coastal region where changing climatic conditions increase the frequency of extreme weather events. Developing urbanization affects the hydrology of natural basins by simplification of the drainage system and reduction of infiltration and base flow. Consequently greater runoff rates flow into storm water collection systems, reservoirs and surrounding water bodies. Not only infrastructures of urban...
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Low-Loss 3D-Printed Waveguide Filters Based on Deformed Dual-Mode Cavity Resonators
PublicationThis paper introduces a new type of waveguide filter with smooth profile, based on specially designed dual-mode (DM) cavity resonators. The DM cavity design is achieved by applying a shape deformation scheme. The coupling between the two orthogonal cavity modes is implemented by breaking the symmetry of the structure, thus eliminating the need for additional coupling elements. The modes operating in the cavity are carefully analyzed...
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Changes in the addiction prevalence in Polish population between 1990-2019: Review of available data
PublicationThe 1989 collapse of the socialist political system in Poland initiated an avalanche of modifications regarding healthcare policy resulting with new institutions and programs dedicated to monitoring and preventing addiction. In the current article, we look at the available data allowing to track changes in (1) the prevalence of exposure to addictive substances and behaviors, and (2) changes of addictions prevalence in Poland...
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Stormwater runoff in the urbanized coastal basin of Gdańsk Urbanized basin of Gdańsk
PublicationAnthropopressure strongly affects the primal water cycle. Alternation of the natural basins imposes changes of drainage patterns, reduction of bioretention, infiltration and base flow. As a result the overland flow predominates and greater runoff rates flow into storm water collection systems and reservoirs. Moreover changing climatic conditions increase the frequency of rapid extreme weather events. Infrastructures of urban areas...
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Integrating Art into Places in Transition - Rose Kennedy Greenway in Boston as a Case Study
PublicationAmong the many projects realized in public spaces, some are truly unique – and these are the ones that build the identity of a place. The aim of this paper is to examine how integrating art and cultural strategies into public space can enhance and reinforce the sense of a place. Particular attention is devoted to urban spaces that we call “places in transition”, where public art improves the city’s imaginative capacity, enlivens...
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Zintegrowane sterowanie systemami zaopatrzenia w wodę pitną
PublicationOptymalne ekonomicznie zaspokojenie w miastach bieżącego zapotrzebowania na wodę pitną tak, aby spełnione były wymagania jakości dostaw oraz jakości samej wody jest złożonym zadaniem. Zarówno aspekty aplikacyjne jak i teoretyczne tego problemu są przedmiotem intensywnych prac prowadzanych w sektorach naukowych i przemysłowych na całym świecie. Prace te finansowane są przez przemysł, komitety naukowe oraz Programy Ramowe Unii Europejskiej....
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IMPROVING THE QUALITY OF EDUCATION THROUGH THE IMPLEMENTATION OF THE DIPLOMAS AND GROUP PROJECTS DURING ENGINEERING STUDIES IN COOPERATION WITH EMPLOYERS
PublicationThe quality of education is one of the most important factors determining the job opportunity for graduates. The authors present the possibility of carrying out the diplomas in cooperation with employers and institutions interested in employment of students who graduated Gdansk University of Technology, Faculty of Civil and Environmental Engineering and University of Warmia and Mazury, Faculty of Geodesy and Land Management (major:...
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IMPROVING THE QUALITY OF EDUCATION THROUGH THE IMPLEMENTATION OF THE DIPLOMAS AND GROUP PROJECTS DURING ENGINEERING STUDIES IN COOPERATION WITH EMPLOYERS
PublicationThe quality of education is one of most important factors determining the job opportunity for graduates. The authors present the possibility of carrying out the diplomas in cooperation with employers and institutions interested in employment of students who graduated Gdansk University of Technology, Faculty of Civil and Environmental Engineering and University of Warmia and Mazury, Faculty of Geodesy and Land Management (major:...