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Search results for: ANTIBIOTIC RESIDUE
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Buckling and shape control of prestressable trusses using optimum number of actuators
PublicationThis paper describes a method to control the nodal displacement of prestressable truss structures within the desired domains. At the same time, the stress in all members is unleashed to take any value between the allowable tensile stress and critical buckling stress. The shape and stresses are controlled by actuating the most active members. The technique considers the members’ initial crookedness, residual stresses, and slenderness...
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Evaluation of reformer tubes degradation after long term operation
PublicationPurpose of this paper is to show the effect of long-term service at elevated temperatures on microstructural changes of cast steel reformer tubes made of the alloy IN-519 (24%Cr, 24%Ni, Nb). The relationship between mechanical properties and microstructure degradation is discussed. Investigations were performed on five tubes taken from ammonia reformer furnace. Tubes worked at temperature of 880C and 3,2 MPa pressure. The operation...
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Fate and significance of phthalates and bisphenol A in liquid by-products generated during municipal solid waste mechanical-biological pre-treatment and disposal
PublicationSamples of liquid by-products generated by municipal solid waste plants (MSWPs) were tested for the presence of phthalates (PAEs) and bisphenol A (BPA). The results indicated that the wastewater generated during mechanical (sorting unit - SU) and biological (composting unit - CU) pre-treatment (MBT) of residual (mixed) solid waste is a significant source of these compounds. The concentrations of PAEs (up to 32222 lg/L) and BPA...
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Experimental research of the effect of face milling strategy on the flatness deviations
PublicationIn this paper the dependencies between face milling strategy of EN AW6082-T6 aluminum alloy samples, with difference thicknesses (6, 8, and 12 mm) and two cold rolling directions, and flatness deviations were presented. Three strategies of milling included different proportions of material removed from both sides of the plates. This approach allowed to control the proportions of residual surface stresses on both sides of the specimens,...
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Single crystal growth and physical properties of MCo2Al9 (M= Sr, Ba)
PublicationSingle crystals of SrCo2Al and BaCo2Al9 were grown using a self-flux method. A LeBail analysis of the powder X-ray diffraction patterns show that both compounds crystallize in a hexagonal (P6/mmm) crystal structure with lattice parameters: a = 7.8995(1) Å, c = 3.9159(1) Å for SrCo2Al9, and a = 7.9162(2) Å, c = 3.9702(1) Å for BaCo2Al9 aluminide. The low temperature analysis of the heat capacity measurements give a Sommerfeld...
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Strength parameters of deltaic soils determined with CPTU, DMT and FVT
PublicationThis paper presents the results of soil investigation in soft, normally consolidated organic soil in the estuary of Vistula river. The analysis concerns clayey mud and peat layers interbedded with loose to medium-dense sands. Several Cone Penetration Tests with pore water measurement (CPTU), Dilatometer Tests (DMT) and Field Vane Tests (FVT) were performed on the testing site. The cone factor Nkt was estimated using the results...
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Biocomposites from recycled resources as candidates for laboratory reference material to validate analytical tools used in organic compounds emissions investigation
PublicationA suitably chosen reference material should meet specific criteria like representing one of the compound classes most commonly occurring in indoor materials as well as having optimal long-term stability during storage and transport to its destination point and having a compact size. The described interdisciplinary pilot research was aimed to develop and characterize a polymer-based candidate for the laboratory reference material...
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Boron-doped carbon nanowalls for fast and direct detection of cytochrome C and ricin by matrix-free laser desorption/ionization mass spectrometry
PublicationDetecting proteins via surface assisted laser desorption/ionization mass spectrometry (SALDI-MS) method is still highly challenging, and only few examples of nanomaterials have been demonstrated to perform such detection so far. In this study, carbon nanowalls (CNWs), vertically aligned graphene sheet-based materials, presenting specific morphology, dimensions, and boron doping levels have shown improved performances for both qualitative...
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Biomass in biogas production: Pretreatment and codigestion
PublicationGlobally, there is increasing awareness that the implementation of ‘waste to energy’ technology is one of the best means to achieve sustainable energy development. The most popular approach is the conversion of organic-rich compounds into clean and renewable products by anaerobic digestion (AD). Biogas can be produced from agricultural residues, municipal/industrial biowastes, and sustainable biomass, especially materials that...
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Lateral forces determine dimensional accuracy of the narrow‑kerf sawing of wood
PublicationThe shrinking global forest area limits the supply of industrially usable raw resources. This, in combination with the ever‑increasing consumption of timber due to population growth can lead to the lack of a positive balance between the annual volumetric growth and consumption of wood. An important innovation toward increasing environmental and economic sustainability of timber production is to reduce the volume of wood residues...
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A comprehensive review on current and emerging technologies toward the valorization of bio‐based wastes and by products from foods
PublicationIndustries in the agro-food sector are the largest generators of waste in the world. Agro-food wastes and by products originate from the natural process of senescence, pretreatment, handling, and manufacturing processes of food and beverage products. Notably, most of the wastes are produced with the transformation of raw materials (such as fruits, vegetables, plants, tubers, cereals, and dairy products) into different processed...
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Novel primosomal protein B from Clostridium pastuerianum
PublicationPriB is a primosomal protein that catalyzes DNA replication in Procaryota. The replication pathway starts with PriA protein - the initiator protein that binds to a DNA replication fork, unwinds double-stranded DNA and role of PriB is to stabilize PriA on the DNA. However there are many biochemical differences in replication mechanism in bacteria and only some of them use PriB proteins. A few of PriB proteins were published and...
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Organic solvents aggregating and shaping structural folding of protein, a case study of the protease enzyme
PublicationLow solubility of reactants or products in aqueous solutions can result in the enzymatic catalytic reactions that can occur in non-aqueous solutions. In current study we investigated aqueous solutions containing different organic solvents / deep eutectic solvents (DESs) that can influence the protease enzyme's activity, structural, and thermal stabilities. Retroviral aspartic protease enzyme is responsible for the cleavage of the...
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Migration of trace elements and radioisotopes to various fractions of solid wastes generated as a result of the sewage sludge incineration process
PublicationThe research was aimed at providing new knowledge in the field of chemical characteristics of solid waste generated in the process of combustion of sewage sludge in fluidized bed furnaces. The research material consisted of disposed fluidized beds (DFB), sewage sludge ash (SSA) and air pollution control residues (APC) from three Polish installations for the thermal treatment of sewage sludge. Natural radionuclides as well as anthropogenic...
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Expression of a GDSL esterase from Pseudomonas sp. S9 in Pichiapastoris
PublicationCold active lipolytic enzymes are promising to replace the conventional enzymes processes of biotechnological industries. One of the most important feature of the cold-active lipases and esterases is that they offer economic benefits through energy saving. In general, they exhibit high activity at low temperatures and low thermostability at moderate temperatures. Lipolytic enzyme EstS9 was isolated from Pseudomonas sp. S9. A multiple...
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Assessment of cytotoxic and endocrine potential of selected xenobiotics commonly present in food products
PublicationMore and more increased intensity of anthropopressure processes can be observed, among other things, in the release of great quantities of synthetic substances into the environment, including the Endocrine Disrupting Compounds (EDC). A vast number of chemical substances is considered to belong to the this group, including substances which occur naturally in the environment, such as mycotoxins and phytoestrogens, and substances...
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GPS-derived height changes in diurnal and sub-diurnal timescales
PublicationThis paper describes the research concerning precise short-time GPS solutions conducted in the Centre of Applied Geomatics, Military University of Technology, Warsaw, Poland. The data from ASG-EUPOS (Polish Active Geodetic Network) was processed using Bernese 5.0 software and EPN (EUREF Permanent Network) standards and models. In this study, the adapted 3-hour observation window is shifted every hour for obtaining hourly geocentric...
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Novel primosomal protein B from Clostridium pasteurianum
PublicationPriB is a primosomal protein that catalyzes DNA replication in Procaryota. The replication pathway starts with PriA protein - the initiator protein that binds to a DNA replication fork, unwinds double-stranded DNA and role of PriB is to stabilize PriA on the DNA. However there are many biochemical differences in replication mechanism in bacteria and only some of them use PriB proteins. A few of PriB proteins were published and...
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MINERAL MATTER IN MUNICIPAL SOLID WASTE
PublicationMunicipal solid waste (MSW) contains mineral materials which are seldom considered as a potential resource. Currently, the waste management sector pays attention to recyclable parts, biodegradable material, waste-to-energy fraction, and residues after waste reuse and recycle. In contrast, this study focus as on the mineral matter in MSW. The aim was to analyze and discuss the sources of mineral matter in MSW, the impact which the...
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Multicopy Suppressor Analysis of Strains Lacking Cytoplasmic Peptidyl-Prolyl cis/trans Isomerases Identifies Three New PPIase Activities in Escherichia coli That Includes the DksA Transcription Factor
PublicationConsistent with a role in catalyzing rate-limiting step of protein folding, removal of genes encoding cytoplasmic protein folding catalysts belonging to the family of peptidyl-prolyl cis/trans isomerases (PPIs) in Escherichia coli confers conditional lethality. To address the molecular basis of the essentiality of PPIs, a multicopy suppressor approach revealed that overexpression of genes encoding chaperones (DnaK/J and GroL/S),...
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Challenges and opportunities related to the use of sewage sludge ash in cement-based building materials – A review
PublicationDue to the increasingly demanding legal regulations, regarding both sewage sludge (SS) and solid residues obtained during its thermal utilization processes, new solutions to limit their negative impact on the environment are sought. One approach that eliminates the risk of sewage sludge ash (SSA) is its use in cement building materials. The article contains a detailed literature review regarding the use of SSA in mortars and concretes,...
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Active Annotation in Evaluating the Credibility of Web-Based Medical Information: Guidelines for Creating Training Data Sets for Machine Learning
PublicationMethods Results Discussion References Abbreviations Copyright Abstract Background: The spread of false medical information on the web is rapidly accelerating. Establishing the credibility of web-based medical information has become a pressing necessity. Machine learning offers a solution that, when properly deployed, can be an effective tool in fighting medical misinformation on the web. Objective: The aim of this study is to...
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Lipopolysaccharides: regulated biosynthesis and structural diversity
PublicationThe cell envelope of Gram-negative bacteria contains two distinct membranes, an inner (IM) and an outer (OM) membrane, separated by the periplasm, a hydrophilic compartment that includes a thin layer of peptidoglycan. The most distinguishing feature of such bacteria is the presence of an asymmetric OM with phospholipids located in the inner leaflet and lipopolysaccharides (LPSs) facing the outer leaflet. The maintenance of this...
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Solar light driven degradation of norfloxacin using as-synthesized Bi 3+ and Fe 2+ co-doped ZnO with the addition of HSO 5 – : Toxicities and degradation pathways investigation
PublicationIn this study, solar light responsive Bi3+ and Fe2+ doped ZnO were synthesized and used for photocatalytic degradation of norfloxacin (NOR), an emerging water pollutant. Analysis with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), selected area electron...
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Membrane Sterols Modulate the Binding Mode of Amphotericin B without Affecting Its Affinity for a Lipid Bilayer
PublicationMembrane-active antibiotics are known to selectively target certain pathogens based on cell membrane properties, such as fluidity, lipid ordering, and phase behavior. These are in turn modulated by the composition of a lipid bilayer and in particular by the presence and type of membrane sterols. Amphotericin B (AmB), the golden standard of antifungal treatment, exhibits higher activity toward ergosterol-rich fungal membranes, which...
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A novel concept of negative CO2 emission power plant for utilization of sewage sludge
PublicationCarbon Capture and Storage (CSS) technology is considered an important “bridge technology”, allowing effective abatement of CO2 emissions for power units using fossil fuels. However, if biomass is used in a power plant, equipped with CCS system, negative CO2 emissions can be achieved. In practice, wide development of such technologies would enable not only reduction of CO2 emissions, but also reversal of the harm done so far. In...
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Kluczowe zagadnienia procesu recyklingu zużytych modułów fotowoltaicznych I i II generacji
PublicationW ramach technologii fotowoltaicznych wyróżniamy obecnie trzy generacje ogniw fotowoltaicznych. Pierwsza z nich to ogniwa z mono- i polikrystalicznego krzemu (c-Si), druga obejmuje ogniwa wykonane na bazie technologii cienkowarstwowej, zarówno z krzemu amorficznego (a-Si) jak i diseleneku indowo-miedziowo-galowego (CIGS), tellurku kadmu (CdTe) oraz arsenku galu (GaAs). Trzecia generacja to najnowsze technologie, takie jak: organiczne...
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THE USE OF MORPHOLOGICAL FILTERS AND GRANULOMETRIC METHOD TO ANALYZE THE MOVEMENT OF THE MOLECULES IN THE SEA WATER OF THE SOUTHERN BALTIC SEA
PublicationModern granulometry opens a new way of analysing images by means of morphological filters. In this paper a closer look has been presented on the problem of interpreting information about the features and conditions of marine environment from images taken at the sea coast and shallow water. Using this method allows for example selection of the objects on the image according to the differences in shape. Processes occurring in the...
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Fatigue Bending of V-Notched Cold-Sprayed FeCoCrNiMn Coatings
PublicationCold-spray coatings were produced by FeCoCrNiMn high-entropy alloy powders deposited on carbon steel substrate. The coatings were realized at intermediate temperature and high pressure (at 1100 °C and 7 MPa). The coating microstructure was characterized by scanning electron microscopy and X-ray diffraction, revealing a very dense deposition and high flattening ratio of the splatted particles. This had a large influence on the...
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Towards an Understanding of the Stability Assessment of Floating Buildings
PublicationOne of the most important aspects of the design of floating facilities such as ships, floating offshore structures or floating houses is stability. Its impact on both general safety and operational aspects renders it a fundamental consideration already in preliminary design stages. Usually, the concept of sufficient stability of floating buildings is associated with the ability to keep an allowed heel angle and residual freeboard,...
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Assessment of metal removal in a surface flow constructed wetland treating landfill leachate
PublicationThe study concerns heavy metal retention and transformation processes in a free water surface constructed wetland treating landfill leachate in a temperate climate. The total effectiveness of heavy metals removal in the analyzed CW was evaluated basing on heavy metals inflow and outflow concentrations. The concentrations of eight heavy metals (Fe, Mn, Zn, Pb, Ni, Cu, Cr, Cd) in landfill leachate (LL) and sediments were measured....
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Metody teorii sterowania w projektowaniu układów diagnostycznych.
PublicationPraca dotyczy zastosowania wybranych metod teorii sterowania do syntezy algorytmów detekcji uszkodzeń. Algorytmy takie, stanowiące istotną gałąź współczesnej diagnostyki technicznej obiektów dynamicznych, w swej istocie opierają się na rozwiązaniach odpowiednich zadań odpornego wyznaczania wektorów resztowych, czyli ważonych błędów oszacowań wyjścia danego obiektu dynamicznego (nadzorowanego procesu). Na treść pracy...
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Control theory methods in diagnostic system design
PublicationPraca dotyczy zastosowania wybranych metod teorii sterowania do syntezy al-gorytmów detekcji uszkodzeń. Algorytmy takie, stanowiące istotną gałąźwspółczesnej diagnostyki technicznej obiektów dynamicznych, w swej istocieopierają się na rozwiązaniach odpowiednich zadań odpornego wyznaczania wek-torów resztowych, czyli ważonych błędów oszacowań wyjściadanego obiektu dy-namicznego (nadzorowanego procesu). Na treść pracy...
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Diffusible Hydrogen Control in Flux Cored Arc Welding Process
PublicationOne of the types of hydrogen degradation of steel welded joints is cold cracking. The direct cause of the formation of cold cracks is simultaneous presence of hydrogen, residual stresses and brittle structure. The way of preventing the occurring of degradation is to eliminate at least one of these factors. Practice has shown that the best solution is to control the amount of hydrogen in deposited metal. In this paper an experimental...
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Preparation and characterization of genipin cross-linked porous chitosan–collagen–gelatin scaffolds using chitosan–CO2 solution
PublicationNovel porous scaffolds composed of chitosan, collagen and gelatin were prepared by the multistep procedure involving final freeze-drying and characterized. To eliminate the need for residual acid removal from the material after drying, carbon dioxide saturation process was used for chitosan blend formulation. The use of CO2 for chitosan dissolution made the scaffold preparation process more reproducible and economically sustainable....
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Nano-structured Pt embedded in acidic salts of heteropolymolybdate matrices: MS EXAFS study
PublicationA structural study of Pt nano-catalysts is presented in the paper. The innovation in the case of the considered catalytic materials resides in the use a meso-microporous inorganic matrix of heteropolyacid salt of composition X2.5H0.5YMo12O40 where X = Rb, Cs and Y = P, Si as a catalyst support. Metallic nanoparticles were created by platinum ions reduction in a hot H2/Argon stream and were mainly located into matrix pores. As the...
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Experimental and numerical investigations of ultimate strength of imperfect stiffened plates of different slenderness
PublicationThe objective of this study is to analyse the behaviour of compressed stiffened plates of different slenderness using experimental and numerical methods. The presented results are part of a long-term project to investigate the ultimate strength of geometrically imperfect structures subjected to different degradation phenomena, including corrosion degradation and locked cracks. Several specimens were subjected to a uniaxial compressive...
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Divulging the anti-acetylcholinesterase activity of Colletotrichum lentis strain KU1 extract as sustainable AChE active site inhibitors
PublicationAlzheimer’s disease (AD), also called senile dementia is a neurodegenerative disease seen commonly in the elderly and is characterised by the formation of β-amyloid plaques and neurofbrillary tangles (NFT). Though a complete understanding of the disease is lacking, recent studies showed the role of the enzyme acetylcholinesterase (AChE) in pathogenesis. Finding new lead compounds from natural sources has always been a quest for...
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Catalysts for advanced oxidation processes: Deep eutectic solvents-assisted synthesis – A review
PublicationNew catalyst synthesis techniques, including green materials, are extensively studied for heterogeneous photocatalytic advanced oxidation processes (AOPs) on spotlight of sustainable development. Deep eutectic solvents (DESs) started to be used in this field as environmentally friendly alternative to ionic liquids (ILs). During the catalyst synthesis, DESs can act as stabilizers, capping agents, structure directing agents, templates,...
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Genotoxicity of selected pharmaceuticals, their binary mixtures, and varying environmental conditions – study with human adenocarcinoma cancer HT29 cell line
PublicationPharmaceutical residues are present in the environment in mixtures and their adverse effects may also result from interactions that occur between compounds. Studies presented in this work focus on genotoxicity of pharmaceuticals from different therapeutic groups in mixtures and in individual solutions impacted with different environmental conditions assessed using comet assay (alkaline approach). Binary mixtures of pharmaceuticals...
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Mechanism of recognition of parallel G-quadruplexes by DEAH/RHAU helicase DHX36 explored by molecular dynamics simulations
PublicationBecause of high stability and slow unfolding rates of G-quadruplexes (G4), cells have evolved specialized helicases that disrupt these non-canonical DNA and RNA structures in an ATP-dependent manner. One example is DHX36, a DEAH-box helicase, which participates in gene expression and replication by recognizing and unwinding parallel G4s. Here, we studied the molecular basis for the high affinity and specificity of DHX36 for parallel-type...
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A review on recent advances in the application of biosurfactants in wastewater treatment
PublicationMicroorganisms produce a variety of non-conventional surface-active molecules, known as biosurfactants. The biosurfactants find diverse applications in the oil industry, agriculture, emulsifiers, and wastewater treatment, to name a few. Since they are produced from microbes, advantages such as biodegradability, lower toxicity, and environmental compatibility can be leveraged compared to the chemical surfactants. Recently, biosurfactants...
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Collagen type II–hyaluronan interactions – the effect of proline hydroxylation: a molecular dynamics study
PublicationHyaluronan–collagen composites have been employed in numerous biomedical applications. Understanding the interactions between hyaluronan and collagen is particularly important in the context of joint cartilage function and the treatment of joint diseases. Many factors affect the affinity of collagen for hyaluronan. One of the important factors is the ratio of 3- or 4-hydroxy proline to proline residues. This article presents...
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Iron–Sulfur Cluster Biogenesis Chaperones: Evidence for Emergence of Mutational Robustness of a Highly Specific Protein–Protein Interaction
PublicationBiogenesis of iron–sulfur clusters (FeS) is a highly conserved process involving Hsp70 and J-protein chaperones. However, Hsp70 specialization differs among species. In most eukaryotes, including Schizosaccharomyces pombe, FeS biogenesis involves interaction between the J-protein Jac1 and the multifunctional Hsp70 Ssc1. But, in Saccharomyces cerevisiae and closely related species, Jac1 interacts with the specialized Hsp70 Ssq1,...
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Novel single-stranded DNA-binding proteins from extreme psychrophilic bacterium Psychromonas ingrahamii 37
PublicationWe report the identification and characterization of the single-stranded DNA binding protein (SSB) from extreme psychrophilic bacterium Psychromonas ingrahamii 37 (PinSSB) that grows expotentially at -12°C and may well grow at even lower temperatures. PinSSB is one of the largest known bacterial SSB protein consisting 222 amino acid residues with a calculated molecular mass of 25.1 kDa. The analysis by gel filtration chromatography...
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Characterization of exceptionally thermostable single-stranded DNA-binding proteins from Thermotoga maritima and Thermotoga neapolitana
PublicationBACKGROUND: In recent years, there has been an increasing interest in SSBs because they find numerous applications in diverse molecular biology and analytical methods.RESULTS: We report the characterization of single-stranded DNA binding proteins (SSBs) from the thermophilic bacteria Thermotoga maritima (TmaSSB) and Thermotoga neapolitana (TneSSB). They are the smallest known bacterial SSB proteins, consisting of 141 and 142 amino...
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Synthesis and biological evaluation of fluorinated N-benzoyl and N-phenylacetoyl derivatives of 3-(4-aminophenyl)-coumarin-7-O-sulfamate as steroid sulfatase inhibitors
PublicationIn the present work, we report convenient methods for the synthesis of 3-(4-aminophenyl)-coumarin-7-O-sulfamate derivatives N-acylated with fluorinated analogues of benzoic or phenylacetic acid as steroid sulfatase (STS) inhibitors. The design of these potential STS inhibitors was supported by molecular modeling techniques. Additionally, computational docking methods were used to determine the binding modes of the synthesized inhibitors...
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Cloning, expression, and biochemical characterization of a coldactive GDSL-esterase of a Pseudomonas sp. S9 isolated from Spitsbergen island soil
PublicationAn estS9 gene, encoding an esterase of the psychrotolerant bacterium Pseudomonas sp. S9 was cloned and sequenced. The deduced sequence revealed a protein of 636 amino acid residues with a molecular mass of 69 kDa.Further amino acid sequence analysis revealed that the EstS9 enzyme contained a G-D-S-L motif centered at a catalytic serine, an N-terminal catalytic domain and a C-terminal autotransporter domain. Two recombinant E. coli...
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BADANIE WPŁYWU INDUKCJI REMANENCJI NA STAN PRZEJŚCIOWY JEDNOFAZOWEGO UKŁADU TRANSFORMATOROWEGO
PublicationW referacie przedstawiono wyniki badań eksperymentalnych i symulacyjnych wpływu indukcji remanencji w rdzeniu transformatora jednofazowego na jego stan przejściowy przy jego pracy jałowej. Badany obiekt jest układem transformatorowym o dwóch uzwojeniach nawiniętych na zwijanym z blachy anizotropowej rdzeniu w kształcie toroidu. Doświadczenia eksperymentalne polegały na rozładowywaniu kondensatora przez uzwojenie strony pierwotnej...
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Cyclic behavior of FeCoCrNiMn high entropy alloy coatings produced through cold spray
PublicationeCoCrNiMn high entropy alloy powders were employed to produce coatings on carbon steel through high pressure cold spray (at 1100 °C and 7 MPa in temperature and pressure respectively). X-ray diffraction of the sprayed material revealed a dense coating with the retention of the original crystallographic structure. Once splatted, particles revealed high flattening ratio with consequent excellent adhesion of the coating to the substrate....