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Wyniki wyszukiwania dla: RAIN
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Rain Water: Atmospheric Deposition
PublikacjaWater is transferred from the atmosphere to the land surface mainly via various forms of atmospheric precipitation; however, the contribution of surface condensation/deposition (e.g., dew, hoar frost, occult precipitation) should not be neglected. Rainwater and liquid deposits (dew, hoar frost, rime) are components of the physical and geographical environment that are easily assimilated and transported. Thus, they are generally...
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Pesticides in rain and roof runoff waters from an urban region
PublikacjaThe levels of organonitrogen, organochlorine and organophosphorus pesticides were monitored in rain and roof runoff waters in Gdask (Poland) over a period of 6 months (October 2000 - March 2001). Buildings included in the study were of different ages, and covered with a variety of roofing materials. Samples were collected during the precipitation events. Pesticides were determined by gas chromatography combined with NPD and ECD...
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Health-Promoting Places: Rain Gardens and Sustainable Water Management
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Simple model of rain wind induced vibrations of stayed cables.
PublikacjaAnalizowano prosty, liniowy model drgań kabli mostów wantowych pod obciążeniem wiatrem i deszczem. Zakłada się, iż częstotliwość ruchu górnej kropli po powierzchni kabla i częstotliwość kabla są równe oraz iż amplituda ruchu kropli dla danej prędkości wiatru są stałe. Otrzymane rezultaty są porównane z istniejącymi w literaturze wynikami badań eksperymentalnych. Podaje się analityczne związki służące do oszacowania tłumienia...
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Technical solutions and benefits of introducing rain gardens – Gdańsk case study
PublikacjaNowadays, Nature-Based Solutions (NBSs) are developing as innovative multifunctional tools to maximize urban ecosystem services such as storm water preservation, reduction of runoff and flood protection, groundwater pollution prevention, biodiversity enhancement, and microclimate control. Gdańsk is one of the first Polish cities to widely introduce rain gardens (one example of an NBS) in different areas such as parks, city center,...
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Evaluation of precipitation measurements obtained from different types of rain gauges
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Acidity trace pollutants of urban rain and roof runoff from selected roof coverings
PublikacjaW pracy przedstawiono dane dotyczące chemizmu opadów atmosferycznych i wód spływnych pobranych na terenie dużej aglomeracji miejskiej. Oznaczane były: pH, aniony - chlorki, azotany i siarczany oraz metale ciężkie - cynk, ołów, miedź, kadm. Próbki były pobierane na terenie Trójmiasta i w Dąbrówce Tczewskiej koło Gdańska z budynków pokrytych nowymi i starymi pokryciami. Badania prowadzono od kwietnia do czerwca 2006.
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Investigation of corrosion behavior of 6060 and 6082 aluminum alloys under simulated acid rain conditions
PublikacjaInstantaneous impedance changes of 6060 and 6082 type aluminum alloys in pure water, and simulated acid rain poured into the pure water was investigated by dynamic electrochemical impedance spectroscopy (DEIS). In this way, the influence of aluminum content on the corrosion behavior of 6060 and 6082 type aluminum alloys immersed in simulated acid rain was determined. The corrosion rate of the alloy decreased with increasing Al...
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Vivedenna dosovih vod u grunt = The rain - water management solutions basedon the seepage phenomena
PublikacjaWprowadzanie wód opadowych do gruntu. Rozwiązania o małej przepustowości, głównie do zastosowania w budynkach jednorodzinnych.
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Vivedenna dosovih vod u grunt = The rain - water management solutions basedon the seepage phenomena cz.2
PublikacjaWprowadzanie wód opadowych do gruntu. Rozwiązania o dużej przepustowości, do użycia w odwadnianiu dużych powierzchni.
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A comprehensive evaluation of mimosa extract as a corrosion inhibitor on AA6060 alloy in acid rain solution: part I. Electrochemical AC methods
PublikacjaThe inhibition effect of mimosa extract on the corrosion of AA6060 aluminum alloy in acid rain solution was investigated by electrochemical impedance spectroscopy and dynamic electrochemical impedance spectroscopy (DEIS). All the studied electrochemical parameters showed good corrosion inhibitive characteristics with respect to the aluminum alloy in the tested solution. Inhibitor efficiency increased with the concentration and...
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Rainscreen panels deformations mods
PublikacjaIn rainscreen panels designing simplified calculation models are used. For plate computation simply supported panels or panels with fixed edges are commonly used. Such approximations are subjected to large errors that lead to inefficient rainscreen panels design. In order to develop a method for determining the optimal geometry of the rainscreen panels, that would take into consideration the real stiffness of both continuous and...
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A decade of Poland`s integrated Road Safety Programme - economic develop-ment vs. road unsafety. W: [CD-ROM]. Conference proceedings. SORIC´02. 2nd Safety on Road Internationaload International Conference. Kingdom of Bah- rain, 21-23 October 2002. Bahrain: CTRS Univ. Bahrain**2002 [8 s. 5 rys. bibliogr. 5 poz. 10 lat Krajowego Programu Bezpieczeństwa Ruchu Drogowego w Polsce - rozwój ekonomiczny a niebezpieczeństwo ruchu.
PublikacjaReferat zawiera analizę doświadczeń z dziesięciu lat pracy oraz wdrożeń Kra-jowego Programu Bezpieczeństwa Ruchu Drogowego GAMBIT. Przedstawia również analizę przyczyn gwałtownego spadku liczby- śmiertelnych ofiar wypadków dro-gowych w Polsce w roku 2001.
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EXTRAORDINARY RAINFALLS IN GDANSK (NORTHERN POLAND) IN THE 21ST CENTURY
PublikacjaIn the 21st century Gdansk had been affected by two rainfall events with a total precipitation amount of over 120 mm each. Rainfall parameters exceeded the regional values of the rain occurring once every 100 years. At the meteorological station of Gdansk University of Technology, July 9th 2001 was recorded 123.5 mm and July 14th 2016, 150.8 mm rainfall (within 24 hours observation period). Despite the same seasons of summer months...
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The influence of boundary conditions on the resistance and stiffness of rainscreen panels
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URBAN POND AS A RAINWATER RECEIVER
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Impact of intense rains and flooding on mercury riverine input to the coastal zone
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Sustainable Rainwater Management Concept in a Housing Estate with a Financial Feasibility Assessment and Motivational Rainwater Fee System Efficiency Analysis
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PROBABLE RAINFALL IN GDAŃSK IN VIEW OF CLIMATE CHANGE
PublikacjaOne of the manifestations of climate changes is the occurrence of a greater number of precipitation events, characterized by greater rain intensity that affects the economic stability of cities. Gdańsk is an example of a city in which such events have occurred since the beginning of the twenty-first century. Due to the altitude differences in the area of Gdańsk city (between –2 m and 180 m a.s.l.), the occurrence of extreme atmospheric...
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DISTRIBUTION OF LIPIDS AND OXIDATIVE CHANGES THEREIN IN PARTICULARIZED PARTS OF RAINBOW TROUT FILLETS
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Disinfection of rainwater as a way to their microbiological stability and safe use
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Chemometric analysis of rainwater and throughfall at several sites in Poland.
PublikacjaPróbki pobrano w stacjach w różnych rejonach Polski. Stacje te były umieszczone na terenach specjalnie chronionych (Wielkopolski Park Narodowy i Ojcowski Park Narodowy) oraz na niechronionych formacjach leśnych (leśnictwo Stara Piła). Celem badań było porównanie parametrów występujących w opadach atmosferycznych pod względem podobieństwa charakterystyki miejsca a także podobieństw i różnic związanych z przejściem wody opadowej...
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Relativity of the simplified runoff calculations for rainwater drainage system
PublikacjaW artykule przedstawiono problem względności wyników uproszczonych obliczeń spływu wód opadowych dla potrzeb systemów kanalizacji deszczowej, wynikającą zarówno z uproszczeń przy wyborze modelu obliczeniowego, jak i dostępności danych i szacunkowości stosowanych współczynników obliczeniowych. Szczególny nacisk został położony na rolę współczynnika spływu oraz sposobu określania czasu koncentracji i czsu trwania deszczu w zlewni....
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Analysis of Rainfall Data of Gdansk Meteorological Station
PublikacjaW pracy przedstawiono wyniki analizy danych opadowych z okresu 51 lat (1951-2008) ze stacji meteorologicznej w Gdańsku. Analizę przeprowadzono zarówno dla sum rocznych opadów jak i sezonowych i miesięcznych. Dokonano również analizy maksymalnych sum dobowych opadów dla każdego miesiąca, badając ich zmienność w wieloleciu.
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EXTREME RAINFALLS AS A CAUSE OF URBAN FLASH FLOODS; A CASE STUDY OF THE ERBIL-KURDISTAN REGION OF IRAQ
PublikacjaAim of the study The current paper aims to give a detailed evaluation and analysis of some extreme rainfall events that happened in the last decade in terms of spatial and temporal rainfall distribution, intensity rate, and exceedance probability. Moreover, it examines the effects of each analysed aspect on the resulting flash floods in the studied area. Material and methods In their glossary of meteorology, American Meteorology...
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High-Resolution Discharge Forecasting for Snowmelt and Rainfall Mixed Events
PublikacjaDischarge events induced by mixture of snowmelt and rainfall are strongly nonlinear due to consequences of rain-on-snow phenomena and snowmelt dependence on energy balance. However, they received relatively little attention, especially in high-resolution discharge forecasting. In this study, we use Random Forests models for 24 h discharge forecasting in 1 h resolution in a 105.9 km 2 urbanized catchment in NE Poland: Biala River....
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Is the microfiltration process suitable as a method of removing suspended solids from rainwater?
PublikacjaDue to climate change and anthropogenic pressure, freshwater availability is declining in areas where it has not been noticeable so far. As a result, the demands for alternative sources of safe drinking water and effective methods of purification are growing. A solution worth considering is the treatment of rainwater by microfiltration. This study presents the results of selected analyses of rainwater runoff, collected from the...
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Wykorzystanie wyników badań próbek materiału biologicznego do oceny ekspozycji zawodowej i endemicznej
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Irrigation Effects of Red Beet as Affected by Rainfall in Different Regions of Poland
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Probabilistic approach of the Upper and Middle Odra basin daily rainfall modeling
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Towards rainfall interception capacity estimation using ALS LiDAR data
PublikacjaIn this study we develop a spatial model for interception capacity of vegetation based on LiDAR data. The study is conducted in the natural wetland river valley dominated meadows, reeds and small bushes. The multiple regression model was chosen to relate the field measurements of interception capacity and LiDAR statistics at 2m grid. The optimal model was chosen by stepwise selection and further manual variables selection resulting...
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Large shopping centres in Poznań as a source of rainwater recovery and irrigation of urban green areas
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Regulated Assembly of LPS, Its Structural Alterations and Cellular Response to LPS Defects
PublikacjaDistinguishing feature of the outer membrane (OM) of Gram-negative bacteria is its asymmetry due to the presence of lipopolysaccharide (LPS) in the outer leaflet of the OM and phospholipids in the inner leaflet. Recent studies have revealed the existence of regulatory controls that ensure a balanced biosynthesis of LPS and phospholipids, both of which are essential for bacterial viability. LPS provides the essential permeability...
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Regulated Control of the Assembly and Diversity of LPS by Noncoding sRNAs
PublikacjaThe outer membrane (OM) of Gram-negative bacteria is asymmetric due to the presence of lipopolysaccharide (LPS) facing the outer leaflet of the OM and phospholipids facing the periplasmic side. LPS is essential for bacterial viability, since it provides a permeability barrier and is a major virulence determinant in pathogenic bacteria. In Escherichia coli, several steps of LPS biosynthesis and assembly are regulated by the RpoE...
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Small regulatory bacterial RNAs regulating the envelope stress response
PublikacjaMost bacteria encode a large repertoire of RNA-based regulatory mechanisms. Recent discoveries have revealed that the expression of many genes is controlled by a plethora of base-pairing noncoding small regulatory RNAs (sRNAs), regulatory RNA-binding proteins and RNA-degrading enzymes. Some of these RNA-based regulated processes respond to stress conditions and are involved in the maintenance of cellular homeostasis. They achieve...
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Corrigendum to “Impact of intense rains and flooding on mercury riverine input to the coastal zone” [Mar. Pollut. Bull. 127 (2018) 593–602]
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Evaluating the Catastrophic Rainfall of 14 July 2016 in the Catchment Basin of the Urbanized Strzyza Stream in Gdańsk, Poland
PublikacjaWe analyzed the causes, the course, and consequences of the extreme precipitation events that occurred 14 July 2016 in the watershed of Strzyza Creek in Gdańsk, Poland. Automated rain gauges located in Strzyża catchment registered a total precipitation lasting about 16 hours – from 129 to 160 mm of rain depth. More in-depth analysis based on rain data collected by Gdańsk University of Technology (GUT) rain station was done. The...
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STORMWATER AS AN ALTERNATIVE WATER SOURCE: QUALITY CHANGES WITH RAINFALL DURATION AND IMPLICATIONS FOR TREATMENT APPROACHES
PublikacjaThe pressure on the world’s water resources is rapidly increasing due to population growth and climatic changes. Valorisation of stormwater as a water resource for non-potable reuse can reduce high-quality water demands and save it for potable uses. In this context, not only roof runoff but also drained stormwater outflow, representing considerably higher pollution levels, should be considered as a potential resource. We analysed...
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Application of Rainfall - Runoff Model for the Analysis of Extreme Outflow from the Upper Strzyża Basin
PublikacjaW pracy przedstawiono zastosowanie hydrologicznego modelu opad - odpływ do wyznaczenia hydrogramu odpływu z górnej części zlewni Potoku Strzyża w Gdańsku. Obliczenia wykonano przy pomocy systemu obliczeniowego HEC-HMS, przyjmując do oszacowania opadu efektywnego metodę SCS-CN. Wykorzystując stworzony model zlewni obliczono odpływ w przekroju zamykającym zlewnię w miejscu lokalizacji zbiornika retencyjnego Nowiec II, który wskutek...
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Rainwater chemistry composition in Bellsund: Sources of elements and deposition discrepancies in the coastal area (SW Spitsbergen, Svalbard)
PublikacjaDiscrepancies in rainfall chemistry in Bellsund were found to be influenced by the orographic barrier and related to the variability in the inflow of air masses as well as to the distance of sampling sites from the sea and thus the extent of sea spray impact. This study covers measurements of rainfall (P) and air temperature (T), physicochemical parameters (pH, specific electrolytic conductivity (SEC), major ions (Cl , NO3 , SO4...
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Lipopolysaccharides: regulated biosynthesis and structural diversity
PublikacjaThe cell envelope of Gram-negative bacteria contains two distinct membranes, an inner (IM) and an outer (OM) membrane, separated by the periplasm, a hydrophilic compartment that includes a thin layer of peptidoglycan. The most distinguishing feature of such bacteria is the presence of an asymmetric OM with phospholipids located in the inner leaflet and lipopolysaccharides (LPSs) facing the outer leaflet. The maintenance of this...
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Phosphorylation‐mediated regulation of heat shock response in Escherichia coli
PublikacjaEscherichia coli has two heat shock regulons under the transcriptional control of Esigma(32) and Esigma(E) RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that mutations in a tyrosine phosphatase-encoding gene led to decreased activity of these heat shock regulons. The...
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Molecular basis of essentiality of early critical steps in the lipopolysaccharide biogenesis in Escherichia coli K-12: requirement of MsbA, cardiolipin, LpxL, LpxM and GcvB
PublikacjaTo identify the physiological factors that limit the growth of Escherichia coli K-12 strains synthesizing minimal lipopolysaccharide (LPS), we describe the first construction of strains devoid of the entire waa locus and concomitantly lacking all three acyltransferases (LpxL/LpxM/LpxP), synthesizing minimal lipid IVA derivatives with a restricted ability to grow at around 21 °C. Suppressors restoring growth up to 37 °C of Δ(gmhD-waaA)...
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Advanced sensitivity analysis of the impact of the temporal distribution and intensity of rainfall on hydrograph parameters in urban catchments
PublikacjaKnowledge of the variability of the hydrograph of outflow from urban catchments is highly important for measurements and evaluation of the operation of sewer networks. Currently, hydrodynamic models are most frequently used for hydrograph modeling. Since a large number of their parameters have to be identified, there may be problems at the calibration stage. Hence, sensitivity analysis is used to limit the number of parameters....
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Predictive Capacity of Rainfall Data to Estimate the Water Needs of Fruit Plants in Water Deficit Areas
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Modelling Wetland Growing Season Rainfall Interception Losses Based on Maximum Canopy Storage Measurements
PublikacjaThis study estimates rainfall interception losses from natural wetland ecosystems based on maximum canopy storage measurements. Rainfall interception losses play an important role in water balance, which is crucial in wetlands, and has not yet been thoroughly studied in relation to this type of ecosystem. Maximum canopy storage was measured using the weight method. Based on these measurements, daily values of interception losses...
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Application of Multinomial Logistic Regression to Model the Impact of Rainfall Genesis on the Performance of Storm Overflows: Case Study
PublikacjaIn this study, a mathematical model was proposed to analyze the performance of storm overfows. The model included the infuence of rainfall genesis on the duration of storm overfow, its volume, and the maximum instantaneous fow. The multinomial logistic regression model, which has not been used so far to model objects located in a stormwater system, was proposed to simulate the duration of storm overfow. The Iman–Conover method,...
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Comparative Analysis of Developed Rainfall Intensity–Duration–Frequency Curves for Erbil with Other Iraqi Urban Areas
PublikacjaRainfall Intensity–Duration–Frequency (IDF) relationships are widely used in water infrastructure design and construction. IDF curves represent the relationship between rainfall intensity, duration, and frequency, and are obtained by analyzing observed data. These relationships are critical for the safe design of flood protection structures, storm sewers, culverts, bridges, etc. In this study, the IDF curves and empirical IDF formulas...
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Flood-Triggering Rainfall and Potential Losses—The Copula-Based Approach on the Example of the Upper Nysa Kłodzka River
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Development of cluster analysis methodology for identification of model rainfall hyetographs and its application at an urban precipitation field scale
PublikacjaDespite growing access to precipitation time series records at a high temporal scale, in hydrology, and particularly urban hydrology, engineers still design and model drainage systems using scenarios of rainfall temporal distributions predefined by means of model hyetographs. This creates the need for the availability of credible statistical methods for the development and verification of already locally applied model hyetographs....
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The impact of hydrological research, municipal authorities, and residents on rainwater management in Gdańsk (Poland) in the process of adapting the city to climate change
PublikacjaAim of the study The city of Gdańsk faces changing climatic conditions that result in a higher frequency of extreme weather events. In response to the increasingly frequent appearance of flash floods, scientific research was carried out on changes in the probability of the occurrence of maximum daily precipitation in Gdańsk. The purpose of this paper is to show the role of hydrological research (science), decisions of local authorities...
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Changes in the EPA and DHA content and lipids quality parameters of rainbow trout (Oncorhynchus mykiss, Walbaum) and carp (Cyprinus carpio, L.) at individual stages of hot smoking
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Checkpoints that regulate balanced biosynthesis of lipopolysaccharide and its essentiality in Escherichia coli
PublikacjaThe outer membrane (OM) of Gram-negative bacteria, such as Escherichia coli, is essential for their viability. Lipopolysaccharide (LPS) constitutes the major component of OM, providing the permeability barrier, and a tight balance exists between LPS and phospholipids amounts as both of these essential components use a common metabolic precursor. Hence, checkpoints are in place, right from the regulation of the first committed step...
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Regulation of the First Committed Step in Lipopolysaccharide Biosynthesis Catalyzed by LpxC Requires the Essential Protein LapC (YejM) and HslVU Protease
PublikacjaWe previously showed that lipopolysaccharide (LPS) assembly requires the essential LapB protein to regulate FtsH-mediated proteolysis of LpxC protein that catalyzes the first committed step in the LPS synthesis. To further understand the essential function of LapB and its role in LpxC turnover, multicopy suppressors of ΔlapB revealed that overproduction of HslV protease subunit prevents its lethality by proteolytic degradation...
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Molecular basis of lipopolysaccharide heterogeneity in Escherichia coli: envelope stress-responsive regulators control the incorporation of glycoforms with a third 3-deoxy-α-D-manno-oct-2-ulosonic acid and rhamnose.
PublikacjaMass spectrometric analyses of lipopolysaccharide (LPS) from isogenic Escherichia coli strains with nonpolar mutations in the waa locus or overexpression of their cognate genes revealed that waaZ and waaS are the structural genes required for the incorporation of the third 3-deoxy-α-D-manno-oct-2-ulosonic acid (Kdo) linked to Kdo disaccharide and rhamnose, respectively. The incorporation of rhamnose requires prior sequential incorporation...
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Assessment of Trace Metals Leaching During Rainfall Events from Building Rooftops with Different Types of Coverage – Case Study
PublikacjaRunoff water is an important medium transporting various types of pollution originating from the atmosphere and washed out from roofing materials. The study presents a quality assessment of runoff from different roofs in the context of trace metal concentrations. The analysed rooftops were covered with copper, tar paper and ceramic tile. The quality of the rain water collected at the same time satisfies the demands of the first...
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Escherichia coli K-12 suppressor-free mutants lacking early glycosyltransferases and late acyltransferases. Minimal lipopolysaccharide structure and induction of envelope stress response.
PublikacjaTo elucidate the minimal lipopolysaccharide (LPS) structure needed for the viability of Escherichia coli, suppressor-free strains lacking either the 3-deoxy-d-manno-oct-2-ulosonic acid transferase waaA gene or derivatives of the heptosyltransferase I waaC deletion with lack of one or all late acyltransferases (lpxL/M/P) and/or various outer membrane biogenesis factors were constructed. Δ(waaC lpxL lpxM lpxP) and waaA mutants exhibited...
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Assembly of Lipopolysaccharide in Escherichia coli Requires the Essential LapB Heat Shock Protein
PublikacjaHere, we describe two new heat shock proteins involved in the assembly of LPS in Escherichia coli, LapA and LapB (lipopolysaccharide assembly protein A and B). lapB mutants were identified based on an increased envelope stress response. Envelope stress responsive pathways control key steps in LPS biogenesis and respond to defects in the LPS assembly. Accordingly, the LPS content in ΔlapB or Δ(lapA lapB) mutants was elevated, with...
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Comparison of conductivity averaging methods for one-dimensional unsaturated flow in layered soils
PublikacjaW artykule przedstawiono numeryczne rozwiązania równania Richardsa, opisującego nieustalony przepływ wody w uwarstwionym gruncie nienasyconym. Przeanalizowano różne metody obliczania średniej przewodności hydraulicznej między węzłami siatki numerycznej oraz różne możliwości położenia węzłów względem granic warstw gruntu. Najlepsze wyniki otrzymano stosując metodę uśredniania stanowiącą adaptację wcześniejszej propozycji jednego...
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Dissection of σE‐dependent cell lysis in Escherichia coli: roles of RpoE regulators RseA, RseB and periplasmic folding catalyst PpiD
PublikacjaTo understand the mechanism of sigma(E)-dependent cell lysis, we examined the consequences of deletion derivatives of rpoE regulators rseA, rseB and rseC on sigma(E) transcription, on levels of free versus membrane-bound sigma(E) and on OMP-biogenesis limiting factor(s) that could impact cell lysis. RT-PCR showed that individual nonpolar DeltarseA and DeltarseB increased the rpoE expression to varying extents, with pronounced induction...
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Identification of Substrates of Cytoplasmic Peptidyl-Prolyl Cis/Trans Isomerases and Their Collective Essentiality in Escherichia Coli
PublikacjaProtein folding often requires molecular chaperones and folding catalysts, such as peptidyl-prolyl cis/trans isomerases (PPIs). The Escherichia coli cytoplasm contains six well-known PPIs, although a requirement of their PPIase activity, the identity of their substrates and relative enzymatic contribution is unknown. Thus, strains lacking all periplasmic and one of the cytoplasmic PPIs were constructed. Measurement of their PPIase...
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Multicopy Suppressor Analysis of Strains Lacking Cytoplasmic Peptidyl-Prolyl cis/trans Isomerases Identifies Three New PPIase Activities in Escherichia coli That Includes the DksA Transcription Factor
PublikacjaConsistent with a role in catalyzing rate-limiting step of protein folding, removal of genes encoding cytoplasmic protein folding catalysts belonging to the family of peptidyl-prolyl cis/trans isomerases (PPIs) in Escherichia coli confers conditional lethality. To address the molecular basis of the essentiality of PPIs, a multicopy suppressor approach revealed that overexpression of genes encoding chaperones (DnaK/J and GroL/S),...
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Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective.
PublikacjaThe RpoE sigma factor is essential for the viability of Escherichia coli. RpoE regulates extracytoplasmic functions including lipopolysaccharide (LPS) translocation and some of its non-stoichiometric modifications. Transcription of the rpoE gene is positively autoregulated by EσE and by unknown mechanisms that control the expression of its distally located promoter(s). Mapping of 5′ ends of rpoE mRNA identified five new transcriptional...
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Suppressors of lapC mutation identify new regulators of LpxC, which mediates the first committed step in lipopolysaccharide biosynthesis
PublikacjaGram-negative bacteria, such as Escherichia coli, are characterized by an asymmetric outer membrane (OM) with lipopolysaccharide (LPS) located in the outer leaflet and phospholipids facing the inner leaflet. E. coli recruits LPS assembly proteins LapB, LapC and LapD in concert with FtsH protease to ensure a balanced biosynthesis of LPS and phospholipids. We recently reported that bacteria either lacking the periplasmic domain of...
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Molecular and structural basis of inner core lipopolysaccharide alterations in Escherichia coli: incorporation of glucuronic acid and phosphoethanolamine in the heptose region.
PublikacjaIt is well established that lipopolysaccharide (LPS) often carries nonstoichiometric substitutions in lipid A and in the inner core. In this work, the molecular basis of inner core alterations and their physiological significance are addressed. A new inner core modification of LPS is described, which arises due to the addition of glucuronic acid on the third heptose with a concomitant loss of phosphate on the second heptose. This...
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A new factor LapD is required for the regulation of LpxC amounts and lipopolysaccharide trafficking
PublikacjaLipopolysaccharide (LPS) constitutes the major component of the outer membrane and is essential for bacteria, such as Escherichia coli. Recent work has revealed the essential roles of LapB and LapC proteins in regulating LPS amounts; although, if any additional partners are involved is unknown. Examination of proteins co-purifying with LapB identified LapD as a new partner. The purification of LapD reveals that it forms a complex...
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Ellipsometric study of carbon nitride films deposited by DC-magnetron sputtering
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Influence of heterogeneous air entry pressure on large scale unsaturated flow in porous media
PublikacjaThe paper presents numerical simulations of water infiltration in unsaturated porous media containing coarse-textured inclusions embed- ded in fine-textured background material. The calculations are performed using the two-phase model for water and air flow and a simplified model known as the Richards equation. It is shown that the Richards equation cannot correctly describe flow in the presence of heterogeneities. How- ever, its...
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Two-phase flow in heterogeneous porous media with non-wetting phase trapping
PublikacjaThis article presents amathematical model describing flowof two fluid phases in a heterogeneous porous medium. Zaproponowano model matematyczny przepływu dwufazowego w drobnoziarnistym ośrodku porowatym zawierającym inkluzje gruboziarniste. Inkluzje charakteryzują się niższą wartością ciśnienia wejścia dla płynu niezwilżającego (np. powietrze, ropa naftowa) niż materiał podstawowy. Podczas nawadniania takiego ośrodka dochodzi do...
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Ellipsometric study of carbon nitride films deposited by DC-magnetron sputtering
PublikacjaWe report the optical properties of a carbon nitride (CNx) film as a function of nitrogen concentration (N/C) of the deposited film. As nitrogen concentration is increased (N/C ratio) in a CNx film, the refractive index and band gap also increase. The real and imaginary parts, n and k (refractive index and extinction coefficient) of the complex refraction index of carbon nitride films were determined by spectroscopic ellipsometry...
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Upscaling unsaturated flow in binary porous media with air entry pressure effects
PublikacjaArtykuł dotyczy przepływu w gruntach nienasyconych, zawierających soczewki (inkluzje) materiału gruboziarnistego. Materiał inkluzji charakteryzuje się niższą wartością ciśnienia wejścia powietrza niż materiał tła. Podczas infiltracji część powietrza zostaje zamknięta w inkluzjach, po całkowitym wypełnieniu wodą materiału tła. Z drugiej strony, drenaż inkluzji rozpoczyna się dopiero wtedy, gdy przekroczone zostanie ciśnienie wejścia...
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Poziomy stężeń lotnych związków chlorowcoorganicznych w wodach opadowych na terenie dużej aglomeracji miejskiej = Measurement of volatile chloroorganic compounds in urban rainwater (Gdańsk, Sopot, Gdynia)
PublikacjaW 105 próbkach opadów atmosferycznych (deszcz, deszcz ze śniegiem) zebranych z terenu Trójmiasta w okresie od listopada 2000 r. do maja 2001 r. oznaczano chloro - i bromopochodne metanu, etanu i etenu (7 różnych związków). Najczęściej wykrywanymi i oznaczanymi były: chloroform i czterochlorek węgla, (bardzo popularne rozpuszczalniki stosowane jako środki chłodzące i czyszczące). Najwyższe stężenia obserwowano w przypadku chloroformu,...
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An evaluation of endemic exposure of citizens living in near a Gdańsk Phosphatic Fertilizer Plant
PublikacjaPrzeprowadzono ocenę wpływu Gdańskich Zakładów Nawozów Fosforowych FOSFORY na mieszkańców różnych dzielnic Gdańska. Oznaczono - za pomocą Neutronowej Analizy Aktywacyjnej (NAA) stężenia Al, As, Au, Br, Ca, Cl, Cu, Dy, I, In, K, La, Mg, Mn, Mo, Na, S, Sb, Sm, U, V, Zn w próbkach włosów i paznokci stóp, zebranych od 22 osób mieszkających w sąsiedztwie Zakładów oraz od jedenastoosobowej grupy kontrolnej. Podstawowe różnice pomiędzy...
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Immunization with an anti-idiotypic antibody against the broadly lipopolysaccharide-reactive antibody WN1 222-5 induces Escherichia coli R3-core-type specific antibodies in rabbits
PublikacjaThe mouse monoclonal antibody (mAb) WN1 222-5 recognizes a carbohydrate epitope in the inner core region of LPS that is shared by all strains of Escherichia coli and Salmonella enterica and is able to neutralize their endotoxic activity in vitro and in vivo. Immunization of mice with mAb WN1 222-5 yielded several anti-idiotypic mAbs one of which (mAb S81-19) competitively inhibited binding of mAb WN1 222-5 to E. coli and Salmonella...
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Modeling two phase flow in large scale fractured porous media with an extended multiple interacting continua method
PublikacjaWe present a two phase flow conceptual model, the corresponding simulator (2pMINC) and a workflow for large-scale fractured reservoirs, based on a continuum fracture approach which uses the multiple interacting continua (MINC) method complemented with an improved upscaling technique. The complex transient behavior of the flow processes in fractured porous media is captured by subgridding the coarse blocks in nested volume elements...
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Role of nitrogen in optical and electrical band gaps of hydrogenated/hydrogen free carbon nitride film
PublikacjaWe report the optical and electrical band gap energy of amorphous hydrogenated carbon nitride (a-HCNx) and carbon nitride (a-CNx) as a function of nitrogen concentration (N/C). The optical band gap of a-HCNx and a-CNx films has been determined by means of Ellipsometry and UV-VIS. Both optical and electrical band gaps increase with elevated nitrogen concentration. Experimentally obtained electrical band gap is compared with the...
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Evaluation of occupational exposure in a slide bearings factory on the basis of urine and blood sample analyses
PublikacjaZbadano wpływ pracy w zakładach łożysk ślizgowych na zdrowie pracowników. Przeprowadzono analizę próbek moczu i krwi pobranych od 42 pracowników i 6 osób zatrudnionych w administracji przedsiębiorstwa, stanowiących grupę kontrolną. Oznaczono zawartość Al, Cu, Pb i Zn we krwi i moczu pracowników dwukrotnie, przed i po terapii za pomocą chelatowania, stosując ICP-MS oraz metodę dodatku wzorca. Istotność różnic w zawartości pierwiastków...
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Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor
PublikacjaEo-E RNA polymerase transcribes a regulon of folding factors for the bacterial envelope and is induced by physical and chemical stresses. The RseA anti-sigma factor inhibits the activity of Esigma(E) RNA, polymerase. It is shown here that the N-terminal portion of sigma(E), residues 1-153, binds core RNA polymerase. RseA interacts with residues 154-191 of sigma(E), a site that is homologous to region 4, the sigma factor binding...
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Nuclear Activation Methods in the Estimation of Environmental Pollution and the Assessment of the Industrial Plant Impact on the Citizens of Gdansk (Poland)
PublikacjaNeutronowa Analiza Aktywacyjna została wykorzystana do oceny stanu jakości powietrza atmosferycznego na podstawie badań analitycznych stopnia kumulacji zanieczyszczeń nieorganicznych w mchu torfowca, a także składu wód opadowych. Ponadto przedstawiono wyniki badań dotyczących szacowania ryzyka zawodowego pracowników zakładów przemysłowych na podstawie analizy próbek materiału biologicznego pobranego od osób narażonych.
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Assessment of atmospheric inorganic pollution in the urban region of Gdańsk, Northern Poland
PublikacjaProjekt wykonano w przemysłowej części Gdańska w celu zidentyfikowania głównych źródeł zanieczyszczeń. Próbki suchego oraz żywego (w hodowli hydroponicznej) mchu eksponowano równolegle w celu określenia ich właściwości kumulacji w aspekcie badań biomonitoringowych. Analizę chemiczną składu próbek mchu wykonano dzięki zastosowaniu techniki aktywacji neutronowej i reaktorze badawczym w Delft (Holandia). Uzyskane wyniki wskazują,...
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Assessment of phosphatic fertilizer production impact on occupational staff based on NAA of hair, nails, and inhald particles
PublikacjaW pracy przedstawiono wyniki badań nad wpływem zakładów fosforowych na zdrowie pracowników. W celu oszacowania narażenia zawodowego oznaczano 25 pierwiastków w próbkach włosów, paznokci i w cząstkach pyłu pobranych od pracowników i od grupy kontrolnej. Otrzymane wyniki badań porównano za pomocą testów statystycznych (t-studenta i nieparametrycznego testu U Manna - Whitneya).
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Crystal structures of the DsbG disulfide isomerase reveal an unstable disulfide
PublikacjaDsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. Here, we present two crystal structures of DsbG at 1.7- and 2.0-Angstrom resolution that are meant to represent the reduced and oxidized forms, respectively. The oxidized structure, however, reveals a mixture...
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Reactive deposition of TiNx layers in a DC-magnetron discharge
PublikacjaTiNx layers have been deposited in 'balanced mode' and 'unbalanced mode' of a reactive DC-magnetron plasma (carrier gas argon, reactive gas nitrogen) under different conditions. Discharge power and reactive gas flow have been varied. The layers have been examined by X-ray photoelectron spectroscopy (XPS), X-ray reflectometry (XR), and spectroscopic ellipsometry (SE). The results of the layer analyses were combined with plasma investigations...
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Optical and chemical characterization of thin TiNx films deposited by DC-magnetron sputtering
PublikacjaThin titanium nitride (tinx) films were deposited on silicon substrates by means of a reactive dc-magnetron plasma. Layers were synthesized under various conditions of discharge power and nitrogen flows in two operation modes of the magnetron (the so-called "balanced" and "unbalanced" modes). The optical constants of the tinx films were investigated by spectroscopic ellipsometry (se). X-ray photoelectron spectroscopy (xps) was...
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Role of nitrogen in evolution of sp2/sp3 bonding and optical band gap in hydrogenated carbon nitride
PublikacjaDrastic changes in the bonding are found in amorphous hydrogenated carbon nitride (a-CNx:H) film as a function of nitrogen concentration (or N/C ratio). The total C-sp3 fraction and hardness shows a sharp decrease (at N/C = 0.40) whereas optical band gap and resistivity shows a gradual increase as nitrogen concentration increases from 0.07 to 0.58. Raman spectrum of a-CNx:H film is fitted with both Gaussian (integrated intensity...
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Deposition and characterization of organic polymer thin films using a dielectric barrier discharge with different C2Hm/N2 (m = 2, 4, 6) gas mixtures
PublikacjaOrganic polymer thin films have been deposited on Si(100) and aluminum coated glass substrates by a dielectric barrier discharge (DBD) operated at medium pressure using different C2Hm/N2 (m = 2, 4, 6) gas mixtures. The deposited films were characterized by various spectroscopic techniques. Fourier transform infrared reflection absorption spectroscopy (FT-IRRAS) revealed the chemical functional groups present in the films. The surface...
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Experimental and Numerical Analysis of Air Trapping in a Porous Medium with Coarse Textured Inclusions
PublikacjaThe paper presents a 2D upward infiltration experiment performed on a model porous medium consisting of fine sand background with two inclusions made of coarser sands. The purpose of the experiment was to investigate the effects of structural air trapping, which occurs during infiltration as a result of heterogeneous material structure. The experiment shows that a significant amount of air becomes trapped in each of the inclusions. Numerical...
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Assessment of the impact of a phosphatic fertilizer plant on the adjecent environment using fuzzy logic
PublikacjaZbadano wpływ zakładów nawozów fosforowych na otaczające środowisko. W próbkach trawy i gleby oraz produktów i produktu ubocznego oznaczono wybrane pierwiastki ziem rzadkich, metale ciężkie i metaloidy. Zbadano rozkład stężeń pierwiastków oznaczonych w próbkach gleby i trawy wzdłuż południowego i wschodniego kierunku, porównując ją ze stężeniami oznaczonymi w glebie i trawie z terenu zakładów oraz w substratach i produktach. Wyniki...
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Fatty acyl benzamido antibacterials based on inhibition of DnaK-catalyzed protein folding
PublikacjaWe have reported that the hsp70 chaperone DnaK from Escherichia coli might assist protein folding by catalyzing the cis/trans isomerization of secondary amide peptide bonds in unfolded or partially folded proteins. In this study a series of fatty acylated benzamido inhibitors of the cis/trans isomerase activity of DnaK was developed and tested for antibacterial effects in E. coli MC4100 cells. Nα-[Tetradecanoyl-(4-aminomethylbenzoyl)]-l-asparagine...
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Dehydration converts DsbG crystal diffraction from low to high resolution
PublikacjaDiffraction quality crystals are essential for crystallographic studies of protein structure, and the production of poorly diffracting crystals is often regarded as a dead end in the process. Here we show a dramatic improvement of poorly diffracting DsbG crystals allowing high-resolution diffraction data measurement. Before dehydration, the crystals are fragile and the diffraction pattern is streaky, extending to 10 Å resolution....
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KU812 basophils express urocortin, CRH-R, MC1R and steroidogenic enzymes and secrete progesterone
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Effect of nitrogen doping on TiOxNy thin film formation at reactive high-power pulsed magnetron sputtering
PublikacjaThe paper is focused on a study of formation of TiOxNy thin films prepared by pulsed magnetron sputtering of metallic Ti target. Oxygen and nitrogen were delivered into the discharge in the form of reactive gases O2 and N2. The films were deposited by high-power impulse magnetron sputtering working with discharge repetition frequency f = 250 Hz at low (p = 0.75 Pa) and high (p = 10 Pa) pressure. The substrates were on floating...
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Influence of reactive oxygen species during deposition of iron oxide films by high power impulse magnetron sputtering.
PublikacjaIron oxide films were deposited using high power impulse magnetron sputtering (HiPIMS) of an iron cathode in an argon/oxygen gas mixture at different gas pressures (0.5~Pa, 1.5~Pa, and 5.0~Pa). The HiPIMS system was operated at a repetition frequency $f = 100$~Hz with a duty cycle of 1~\%. A main goal is a comparison of film growth during conventional and electron cyclotron wave resonance-assisted HiPIMS. The deposition plasma...
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Discussion:Horizontal stress increase induced by deep vibratory compaction
PublikacjaDeep compaction control of granular material using the results of field tests. The analysis include the CPTU and DMT tests terformed before and after compaction works.
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Spectroscopic Study of Plasma Polymerized a-C:H Films Deposited by a Dielectric Barrier Discharge
PublikacjaPlasma polymerized a-C:H thin films have been deposited on Si (100) and aluminum coated glass substrates by a dielectric barrier discharge (DBD) operated at medium pressure using C2Hm/Ar (m = 2, 4, 6) gas mixtures. The deposited films were characterized by Fourier transform infrared reflection absorption spectroscopy (FT-IRRAS), Raman spectroscopy, and ellipsometry. FT-IRRAS revealed the presence of sp3 and sp2 C–H stretching and...
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Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells
PublikacjaThe specific role of size scale, surface capping, and aspect ratio of zinc oxide (ZnO) particles on toxicity toward prokaryotic and eukaryotic cells was investigated. ZnO nano and microparticles of controlled size and mor- phology were synthesized by wet chemical methods. Cytotoxicity toward mammalian cells was studied using a human osteoblast cancer cell line and antibacterial activity using Gram-negative bacteria (Escherichia...
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A mobile loop order–disorder transition modulates the speed of chaperonin cycling
PublikacjaMolecular machines order and disorder polypeptides as they form and dissolve large intermolecular interfaces, but the biological significance of coupled ordering and binding has been established in few, if any, macromolecular systems. The ordering and binding of GroES co-chaperonin mobile loops accompany an ATP-dependent conformational change in the GroEL chaperonin that promotes client protein folding. Following ATP hydrolysis,...
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Effect of nitrogen doping on TiOxNythin film formation at reactive high-power pulsed magnetron sputtering
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Plasma diagnostics of low pressure high power impulse magnetron sputtering assisted by electron cyclotron wave resonance plasma
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Effect of mid-frequency discharge assistance on dual-high power impulse magnetron sputtering
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