Filters
total: 910
-
Catalog
- Publications 755 available results
- Journals 12 available results
- Conferences 1 available results
- People 21 available results
- Inventions 11 available results
- Projects 11 available results
- Laboratories 1 available results
- Research Teams 2 available results
- e-Learning Courses 20 available results
- Open Research Data 76 available results
Search results for: dna
-
A Semiautomatic Experience-Based Tool for Solving Product Innovation Problem
PublicationIn this paper we present the idea of Smart Innovation Engineering (SIE) System and its implementation methodology. The SIE system is semi-automatic system that helps in carrying the process of product innovation. It collects the experiential knowledge from the formal decisional events. This experiential knowledge is collected from the group of similar products having some common functions and features. The SIE system behaves like...
-
Manufacturing Data Analysis in Internet of Things/Internet of Data (IoT/IoD) Scenario
PublicationComputer integrated manufacturing (CIM) has enormous benefits as it increases the rate of production, reduces errors and production waste, and streamlines manufacturing sub-systems. However, there are some new challenges related to CIM operating in the Internet of Things/Internet of Data (IoT/IoD) scenarios associated with Industry 4.0 and cyber-physical systems. The main challenge is to deal with the massive volume of data flowing...
-
Stable nanoconjugates of transferrin with alloyed quaternary nanocrystals Ag–In–Zn–S as a biological entity for tumor recognition
PublicationOne way to limit the negative effects of anti-tumor drugs on healthy cells is targeted therapy employing functionalized drug carriers. Here we present a biocompatible and stable nanoconjugate of transferrin anchored to Ag-In-Zn-S quantum dots modified with 11-mercaptoundecanoic acid (Tf-QD) as a drug carrier versus typical anticancer drug, doxorubicin. Detailed investigations of Tf-QD nanoconjugates without and with doxorubicin...
-
Antitumour imidazoacridone C-1311 induces cell death by mitotic catastrophe in human colon carcinoma cells
PublicationW przedstawionych badaniach określano mechanizm śmierci komórkowej indukowanej przez imidazoakrydon C-1311 (Symadex) w komórkach HT-29 ludzkiego raka jelita grubego, które wykazywały szczególną wrażliwość na ten związek w eksperymentalnych modelach in vitro i in vivo. Przy stężeniu odpowiadającemu dawce IC99, związek powodował zahamowanie progresji cyklu komórkowej w fazie S po czym następował blok w fazie G2. Po czasach 48-96...
-
Detection of Toxoplasma gondii Infection in Small Ruminants: Old Problems, and Current Solutions
PublicationToxoplasmosis is a parasitic zoonosis of veterinary importance, with implications for public health. Toxoplasma gondii infection causes abortion or congenital disease in small ruminants. Moreover, the consumption of infected meat, cured meat products, or unpasteurized milk and dairy products can facilitate zoonotic transmission. Serological studies conducted in various European countries have shown the high seroprevalence of specific...
-
Quinoline-based thiazolyl-hydrazones target cancer cells through autophagy inhibition
PublicationHeterocyclic pharmacophores such as thiazole and quinoline rings have a significant role in medicinal chemistry. They are considered privileged structures since they constitute several Food and Drug Administration (FDA)-approved drugs for cancer treatment. Herein, we report the synthesis, in silico evaluation of the ADMET profiles, and in vitro investigation of the anticancer activity of a series of novel thiazolyl-hydrazones based...
-
Effect of Ethanol Extracts of Propolis (EEPs) against Staphylococcal Biofilm—Microscopic Studies
PublicationStaphylococci growing in the form of biofilm exhibit high resistance to a plethora of antibiotics. The aim of the study was to assess the influence of ethanolic extract of propolis (EEPs) on S. epidermidis ATCC 35984 biofilm using fluorescent microscopy. Propidium iodide (PI) and SYTO 9 were used for differentiation of live and dead cells, and calcofluor white was used to stain the extracellular matrix, the self-produced extracellular...
-
Chemical and biological evaluation of antioxidant activity of endogenous redox-active compounds compared to plant-derived exogenous antioxidants
PublicationThe research conducted so far has shown that endogenous antioxidants, despite being regarded as the first line of antioxidant defense, may not be sufficient to maintain redox homeostasis in cells exposed to oxidative stress. The results obtained in the doctoral dissertation show that endogenous redox-active compounds were moderate or weak scavengers of ABTS and DPPH radicals, while in cellular setting, their impact on the reducing...
-
Systemy ekspresji genów - Wykład - 2021_22
e-Learning CoursesTematyka wykładów kursu przedstawia następujące treści: Ekspresja genów w organizmach żywych. Źródła informacji:gdy sekwencja genu jest znana, gdy sekwencja genu nie jest znana, bazy danych, biblioteki DNA genomowego, biblioteki cDNA, narzędzia on-line używane do analizy sekwencji DNA. System ekspresyjny genów - pojęcia podstawowe. Kryteria wyboru systemu ekspresyjnego do produkcji białek heterogenicznych w zależności od ich...
-
Modyfikacja β-cyklodekstryny w celu uzyskania pH czułego nośnika leków antracyklinowych
PublicationW 2012 roku 68% zgonów było spowodowanych przez choroby niezakaźne takie, jak nowotwory, choroby układu krążenia, przewlekłe choroby układu oddechowego [1]. Zgodnie z danymi Światowej Organizacji Zdrowia (ang. World Health Organization, WHO) w 2012 roku 32,6 miliona osób cierpiało z powodu chorób nowotworowych, a 8,2 miliona zmarło z ich powodu [2]. Szacowane jest, że z roku na rok odsetek osób chorujących na choroby nowotworowe...
-
Sialoadenitis - Unknown, 62 - Tissue image [330073006938591]
Open Research DataThis is the histopathological image of OTHER AND UNSPECIFIED MAJOR SALIVARY GLANDS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
-
FLOOR OF MOUTH, Floor of mouth NOS - Unknown, 62 - Tissue image [3300730069387101]
Open Research DataThis is the histopathological image of FLOOR OF MOUTH tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
-
Sialoadenitis - Unknown, 62 - Tissue image [330073006938141]
Open Research DataThis is the histopathological image of OTHER AND UNSPECIFIED MAJOR SALIVARY GLANDS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
-
Sialoadenitis - Unknown, 62 - Tissue image [3300730069385951]
Open Research DataThis is the histopathological image of OTHER AND UNSPECIFIED MAJOR SALIVARY GLANDS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
-
Squamous cell carcinoma, keratinizing, NOS - Unknown, 62 - Tissue image [3300730069381741]
Open Research DataThis is the histopathological image of FLOOR OF MOUTH tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
-
Squamous cell carcinoma, keratinizing, NOS - Unknown, 62 - Tissue image [3300730069382581]
Open Research DataThis is the histopathological image of FLOOR OF MOUTH tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
-
Squamous cell carcinoma, keratinizing, NOS - Unknown, 62 - Tissue image [330073006938211]
Open Research DataThis is the histopathological image of FLOOR OF MOUTH tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
-
Detection of Blastocystis hominis in unpreserved stool specimens byusing polymerase chain reaction
PublicationBlastocystis hominis jest pasożytem występującym na całym świecie. Patogenność tego pasożyta nie jest jeszcze w pełni udowodniona, aczkolwiek opisanych jest wiele przypadków, sugerujących, że B. hominis może wywoływać objawy żołądkowo-jelitowe. Wykrywanie tego pasożyta opiera się na bezpośredniej obserwacji mikroskopowej rozmazów i zagęszczonych próbek kału lub rozmazów utrwalonych, jednakże w pewnych przypadkach nie jest to możliwe.Celem...
-
Conservation and diversity of MutS proteins
PublicationThe homologues of MutS, mismatch repair protein, exist in all prokaryotes, with the exception of Actinobacteria, Mollicutes and part of the Archaea. Multiple alignments of 316 MutS amino acid sequences from 169 species revealed conserved residues and sequence motifs distinguishing MutS homologues. All MutS homologues show high conservation within the ATPase domain. MutS1, the homologue responsible for DNA mismatch recognition,...
-
Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy
PublicationThe present work describes an impedimetric immunosensor for Pseudomonas syringae pv. lachrymans (Psl) detection. This pathogen infects many crop species causing considerable yield losses, thus fast and cheap detection method is in high demand. In the assay, the gold disc electrode was modified with 4-aminothiophenol (4-ATP), glutaraldehyde (GA), and anti-Psl antibodies, and free-sites were blocked with bovine serum albumin (BSA)....
-
Interactions between polyphenolic antioxidants quercetin and naringenin dictate the distinctive redox-related chemical and biological behaviour of their mixtures
PublicationFood synergy concept is suggested to explain observations that isolated antioxidants are less bioactive than real foods containing them. However, mechanisms behind this discrepancy were hardly studied. Here, we demonstrate the profound impact of interactions between two common food flavonoids (individual: aglycones quercetin—Q and naringenin—N− or their glycosides rutin—R and naringin—N+ vs. mixed: QN− and RN+) on their electrochemical...
-
Akustyczne zobrazowanie morskich osadów dennych
PublicationPrzedstawiany temat rozprawy doktorskiej: „Akustyczne zobrazowanie morskich osadów dennych”, prezentuje metody zobrazowania struktury osadów dennych pozyskanych z sondowania akwenu echosondą parametryczną SES-2000 Standard oraz urządzeniami pomocniczym — echosondą wielowiązkową EM3002 i sonarem bocznym. Tezą jest wykazanie, że metody akustyczne dają możliwość bezinwazyjnej klasyfikacji osadów dennych. Hipoteza zakłada, że istnieje...
-
Extracting functional groups of ALLINI to design derivatives of FDA‐approved drugs: Inhibition of HIV‐1 integrase
PublicationHIV‐1 integrase (IN) is crucial for integration of viral DNA into the host genome and a promising target in development of antiretroviral inhibitors. In this work, six new compounds were designed by linking the structures of two different class of HIV‐1 IN inhibitors (active site binders and allosteric IN inhibitors (ALLINIs)). Among newly designed compounds, INRAT10b was found most potent HIV‐1 IN inhibitor considering different...
-
Examination of epigenetic inhibitor zebularine in treatment of skin wounds in healthy and diabetic mice
PublicationDNA methyltransferase inhibitor zebularine was proven to induce regeneration in the ear pinna in mice. We utilized a dorsal skin wound model to further evaluate this epigenetic inhibitor in wound healing. Full-thickness excisional wounds were made on the dorsum of 2 and 10-month-old healthy BALB/c and 3 and 8-month-old diabetic (db/db) mice, followed by topical or intraperitoneal zebularine delivery. Depending on the strain, age,...
-
Techniki Amplifikacji Kwasów Nukleinowych -2023-2024
e-Learning CoursesSpecjalność: Biotechnologia molekularna (WCh), II stopnia, stacjonarne Prowadzący wykład: dr hab. Beata Krawczyk, prof. uczelni - forma stacjonarna Prowadzący laboratoria: mgr inż. Bartosz Ostrowski - zajęcia stacjonarne na PG, sala 209 WCH B (bud.7); II piętro. Kontakt: (58) 347-23-83 Beata Krawczyk (wykłady): e-mail: beata.krawczyk@pg.edu.pl; WCH B; p.217;tel. (58) 347-23-83 Bartosz Ostrowski, WCH B, p.218 tel. (58) 347-13-83 Konsultacje:...
-
A nosocomial outbreak of Candida parapsilosis in southern Sweden verified by genotyping
PublicationIn a haematology ward, Candida parapsilosis was found in blood cultures from 4 patients within a month. As C. parapsilosis is known to have a restricted genetic diversity, a combined methodological approach was adopted to establish a possible epidemiological relationship among the isolates (n = 9). Multilocus sequence typing and random amplified polymorphic DNA analysis suggested a clonal origin of the isolates. The clonal origin...
-
Stream Reasoning to Improve Decision-Making in Cognitive Systems
PublicationABSTRACT Cognitive Vision Systems have gained a lot of interest from industry and academia recently, due to their potential to revolutionize human life as they are designed to work under complex scenes, adapting to a range of unforeseen situations, changing accordingly to new scenarios and exhibiting prospective behavior. The combination of these properties aims to mimic the human capabilities and create more intelligent and efficient...
-
Image Representation for Cognitive Systems Using SOEKS and DDNA: A Case Study for PPE Compliance
PublicationCognitive Vision Systems have gained significant interest from academia and industry during the past few decade, and one of the main reasons behind this is the potential of such technologies to revolutionize human life as they intend to work under complex visual scenes, adapting to a comprehensive range of unforeseen changes, and exhibiting prospective behavior. The combination of these properties aims to mimic the human capabilities...
-
Regenerative Drug Discovery Using Ear Pinna Punch Wound Model in Mice
PublicationThe ear pinna is a complex tissue consisting of the dermis, cartilage, muscles, vessels, and nerves. Ear pinna healing is a model of regeneration in mammals. In some mammals, including rabbits, punch wounds in the ear pinna close spontaneously; in common-use laboratory mice, they remain for life. Agents inducing ear pinna healing are potential regenerative drugs. We tested the effects of selected bioactive agents on 2 mm ear pinna...
-
Multi-Technique Investigation of Grave Robes from 17th and 18th Century Crypts Using Combined Spectroscopic, Spectrometric Techniques, and New-Generation Sequencing
PublicationThe textile fragments of the funeral clothes found in the 17th and 18th century crypts were subjected to spectroscopic, spectrometric, and microbial investigation. The next-generation sequencing enabled DNA identification of microorganisms at the genus and in five cases to the species level. The soft hydrofluoric acid extraction method was optimized to isolate different classes of dyes from samples that had direct contact with...
-
Discovery of small-molecule regenerative drugs using a model of complex tissue injury in mice. Transcriptomic responses of neurodevelopmental genes during pharmacologically induced regeneration
PublicationDespite significant development in regenerative medicine, there is a deficit of effective therapies for wound healing and tissue regeneration. Research performed using animal wound models allows for a better understanding of this complex process, searching for compounds with pro-regenerative properties, and assessing their efficacy and safety. Unfortunately, the lack of adequate preclinical models proves to be an issue as no animal...
-
Thermodynamic, Anticoagulant, and Antiproliferative Properties of Thrombin Binding Aptamer Containing Novel UNA Derivative
PublicationThrombin is a serine protease that plays a crucial role in hemostasis, fibrinolysis, cell proliferation, and migration. Thrombin binding aptamer (TBA) is able to inhibit the activity of thrombin molecule via binding to its exosite I. This 15-nt DNA oligonucleotide forms an intramolecular, antiparallel G-quadruplex structure with a chair-like conformation. In this paper, we report on our investigations on the influence of certain...
-
The structurally similar TRFH domain of TRF1 and TRF2 dimers shows distinct behaviour towards TIN2
PublicationThe telomere repeat binding-factor 1 and 2 (TRF1 and TRF2) proteins of the shelterin complex bind to duplex telomeric DNA as homodimers, and the homodimerization is mediated by their TRFH (TRF-homology) domains. We performed molecular dynamic (MD) simulations of the dimer forms of TRF1TRFH and TRF2TRFH in the presence/absence of the TIN2TBM (TIN2, TRF-interacting nuclear protein 2, TBM, TRF-binding motif) peptide. The MD results...
-
Contextual Knowledge to Enhance Workplace Hazard Recognition and Interpretation in a Cognitive Vision Platform
PublicationThe combination of vision and sensor data together with the resulting necessity for formal representations builds a central component of an autonomous Cyber Physical System for detection and tracking of laborers in workplaces environments. This system must be adaptable and perceive the environment as automatically as possible, performing in a variety of plants and scenes without the necessity of recoding the application for each...
-
Evaluation of a PCR Melting Profile method for intra-species differentiation of Trichophyton rubrum and Trichophyton interdigitale
PublicationW celu identyfikacji źródła infekcji spowodowanych przez dermatofity, jak i drogi ich transmisji, istnieje potrzeba szukania nowych metod, które pozwolą na głębokie zróżnicowanie genetyczne szczepów w obrębie rodzaju. W pracy po raz pierwszy pokazano zastosowanie techniki PCR MP opartej o adaptory oligonukleotydowe i różnice w temperaturze topnienia fragmentów restrykcyjnych DNA w reakcji PCR do wewnątrzgatunkowego typowania dermatofitów....
-
Rola polimorfizmu i zróżnicowanej ekspresji genów cytochromu P450 w metabolizmie ksenobiotyków.
PublicationZdolność do metabolizmu ksenobiotyków przez cytochromy P450 (CYP) określona jest przez polimorfizm i poziom ekspresji genów tych białek. Najwięcej, 40 odmian polimorficznych o zróżnicowanej aktywności enzymatycznej, poznano dla CYP2D6, natomiast tylko dwie w przypadku CYP2C9, których obecność powoduje zahamowanie metabolizmu wielu leków. Z kolei polimorfizm genu CYP3A4 nie miał istotnego wpływu na aktywność metaboliczną jego produktu...
-
A systematic review on cellular responses of Escherichia coli to nonthermal electromagnetic irradiation
PublicationInvestigation of Escherichia coli under electromagnetic fields is of significance in human studies owing to its short doubling time and human‐like DNA mechanisms. The present review aims to systematically evaluate the literature to conclude causality between 0 and 300 GHz electromagnetic fields and biological effects in E. coli. To that end, the OHAT methodology and risk of bias tool were employed. Exponentially growing cells exposed...
-
Relationship between Chemical Structure and Biological Activity Evaluated In Vitro for Six Anthocyanidins Most Commonly Occurring in Edible Plants
PublicationNumerous studies have provided evidence that diets rich in anthocyanins show a broad spectrum of health benefits. Anthocyanins in nature are usually found in the form of glycosides. Their aglycone forms are called anthocyanidins. The chemical structure of anthocyanins is based on the flavylium cation, but they differ in the position and number of substituents. However, the bioactives and foods that contain them are frequently treated...
-
Telomerase inhibition - unfulfilled hopes in the perfect molecular target
PublicationTelomerase plays a pivotal role in cell proliferation, homeostasis, and neoplastic transformation, making it a promising molecular target for cancer chemotherapy. Of note, although hTERT has been explored thoroughly as a target, none of the promising molecules has been approved as a drug until now. The subject of research conducted as part of my doctoral dissertation is explaining the cellular and molecular mechanism of action...
-
Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair
PublicationRegeneration and wound healing are vital to tissue homeostasis and organism survival. One of the biggest challenges of today’s science and medicine is finding methods and factors to stimulate these processes in the human body. Effective solutions to promote regenerative responses will accelerate advances in tissue engineering, regenerative medicine, transplantology, and a number of other clinical specialties. In this study, we...
-
Kinetics of molecular decomposition under irradiation of gold nanoparticles with nanosecond laser pulses—A 5-Bromouracil case study
PublicationABSTRACT Laser illuminated gold nanoparticles (AuNPs) efficiently absorb light and heat up the surrounding medium, leading to versatile applications ranging from plasmonic catalysis to cancer photothermal therapy. Therefore, an in-depth understanding of the thermal, optical, and electron induced reaction pathways is required. Here, the electrophilic DNA nucleobase analog 5-Bromouracil (BrU) has been used as a model compound to...
-
Smart Virtual Product Development (SVPD) System to Support Product Inspection Planning in Industry 4.0
PublicationThis paper presents the idea of supporting product inspection planning process during the early stages of product life cycle for the experts working on product development. Aim of this research is to assist a collaborative product development process by using Smart Virtual Product Development (SVPD) system, which is based on Set of Experience Knowledge Structure (SOEKS) and Decisional DNA (DDNA). The proposed system is developed...
-
Antifungal Activity of Capridine β as a Consequence of Its Biotransformation into Metabolite Affecting Yeast Topoisomerase II Activity
PublicationIn the last few years,increasing importance is attached to problems caused by fungal pathogens. Current methods of preventing fungal infections remain unsatisfactory. There are several antifungal compounds whichare highly effective in some cases, however, they have limitations in usage: Nephrotoxicity and other adverse effects. In addition, the frequent use of available fungistatic drugs promotes drug resistance....
-
IFI16 Impacts Metabolic Reprogramming during Human Cytomegalovirus Infection
PublicationCellular lipid metabolism plays a pivotal role in human cytomegalovirus (HCMV) infection, as increased lipogenesis in HCMV-infected cells favors the envelopment of newly synthesized viral particles. As all cells are equipped with restriction factors (RFs) able to exert a protective effect against invading pathogens, we asked whether a similar defense mechanism would also be in place to preserve the metabolic compartment from HCMV...
-
Effect of oxidation and in vitro intestinal hydrolysis on phospholipid toxicity towards HT29 cell line serving as a model of human intestinal epithelium
PublicationOxidation of food-derived phospholipids (PLs) can influence nutrient digestion and induce oxidative stress in gastrointestinal epithelium. In this study, hen egg yolk PL fraction was used to evaluate the effect of lipoxygenase (LOX)-induced PL oxidation on the rate of PL hydrolysis catalyzed by pancreatic phospholipase A2 (PLA2) in the presence of bile salts (BSs). Then, PL/BS solutions containing native or oxidized PLs were used...
-
The effect of sunscreen 4‐methylbenzylidene camphor in different and reproductive models, its bioaccumulation and molecular effects on ligand‐receptor interaction, and protein expression
Publication4-Methylbenzylidene camphor (4-MBC) is a photo-absorbing UV filter prevalently used in cosmetics, which can be absorbed into circulation and cause systemic effects. 4-MBC is continued to be released in the environment despite the growing knowledge about its bioaccumulation and endocrine disrupting effects. Previous reviews have mentioned UV-filter together but this review considers 4-MBC alone, due to its prevalence and concerning...
-
The increased level of cytochrome P450 isoenzyme CYP3A4 affects the cellular response in CHO cells upon antitumor imidazoacridinone C-1311 treatment
PublicationPochodna C-1311 to potencjalny związek przeciwnowotworowy, który dotarł do II fazy badań klinicznych. Związek ten ulega metabolicznej transformacji pod wpływem szczurzych i ludzkich białek mikrosomalnych. Wykazano, że białka cytochromu P450 odpowiadają za przemiany metaboliczne C-1311 jedynie w niewielkim stopniu. Z drugiej strony wykazano, że pochodna C-1311 jest inhibitorem białek cytochromu P450, zwłaszcza izoenzymu CYP1A2...
-
Squamous cell carcinoma, keratinizing, NOS - Unknown, 62 - Tissue image [330073006938511]
Open Research DataThis is the histopathological image of FLOOR OF MOUTH tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
-
Joanna Raczek dr inż.
PeopleEmployment 2003 -- 2019: Faculty of Applied Physics and Mathematics, Gdańsk University of Technology. 2019 - present: Faculty of Electronic, Informatics and Telecominications, Gdańsk University of Technology. Education May 2007: Doctor of Philosophy in Mathematics, University of Gdańsk. Doctoral dissertation: "Paired domination and doubly domination in graphs". Supervisor: dr hab. Jerzy Topp. 2000 -- 2004 Bachelor of Science...
-
Molecular characterization of a cryptic plasmid from the psychrotrophic antarctic bacterium Pseudoalteromonas sp. 643A
PublicationW ostatnich latach znacznie wzrosło zainteresowanie organizmami zimnolubnymi, głównie z powodu ich enzymów o biotechnologicznym znaczeniu. Jednak, aby wydajnie produkować takie enzymy w skali przemysłowej użyteczne jest stworzenie rekombinantowych systemów ekspresyjnych, które pozwolą na ich opłacalną produkcję. W osiągnięciu tego celu mogą pomóc odpowiednie wektory, konstruowane na bazie plazmidów pozyskiwanych z bakterii psychrofilnych/psychrotrofowych....
-
CYP3A4 overexpression enhances apoptosis induced by anticancer agent imidazoacridinone C-1311, but does not change the metabolism of C-1311 in CHO cells
PublicationWe examine whether CYP3A4 overexpression influences the rate and pattern of antitumor imidazoacridinone C-1311 metabolism, in relation to the impact of this overexpression on cell cycle progression and final cellular response of CHO cells following C-1311 treatment. Methods: Three CHO cell lines: CHO-WT, wild type, CHO-HR, overexpressing cytochrome P450 reductase (CPR) and CHO-HR-3A4, coexpressing CPR and CYP3A4 were applied....
-
Enzymes of the lysine biosynthetic pathway as targets for antifungals ?
PublicationSystemic infections caused by human pathogenic fungi in immunocompromized patients continue to be one of the important clinical problems. Limited availability of safe and efficacious antifungal chemotherapeutics and emerging resistance to existing drugs stimulates search for novel molecular targets for antifungals. The α-aminoadipate pathway (AAP) of L-lysine biosynthesis is unique in fungi and thus has been so far considered...
-
Bioaccumulation of Metals in Tissues of Marine Animals, Part I: the Role and Impact of Heavy Metals on Organisms
PublicationHeavy metals contribute to the anthropogenic contamination of marine ecosystems. Some of them are essential to the life processes of organisms; others are toxic, even at low concentrations. They penetrate organisms via food, respiratory pathways or the skin. The extent to which metals penetrate organisms is measured by the bioconcentration and bioaccumulation factors and also by their transport between organisms at different trophic...
-
Metabolic transformation of antitumor acridinone C-1305 but not C-1311 via selective cellular expression of UGT1A10 increases cytotoxic response: implications for clinical use.
PublicationThe acridinone derivates C-1305 and C-1311 are promising antitumor agents with high activity against several experimental cellular and tumor models and which are under evaluation in pre-clinical and early phase clinical trials. Recent evidence from our laboratories has indicated that both compounds were conjugated by several UGT isoforms with the most active being extrahepatic UGT1A10. The present studies were designed to test...
-
Evaluation of possibilities of genotyping of Candida glabrata clinical isolates with RAPD-PCR method and microsatellite analysis
PublicationThe aim of the study was to compare the discriminatory power of RAPD-PCR method using RSD10 primer and microsatellite (GACA)4 analysis for genotyping clinical isolates of C. glabrata. Isolates were received from patients of two Polish hospitals: Children's Memorial Health Institute in Warsaw (n=17) and Medical University of Gdansk (n=37). Species identification was confirmed by two phenotypic methods: growth on chromagar plates...
-
Imunofan - RDKVYR peptide - stimulates skin cell proliferation and promotes tissue repair
PublicationRegeneration and wound healing are vital to tissue homeostasis and organism survival. One of the biggest challenges of today's science and medicine is finding methods and factors to stimulate these processes in the human body. Effective solutions to promote regenerative responses will accelerate advances in tissue engineering, regenerative medicine, transplantology, and a number of other clinical...
-
Novel staphylococci nucH taxonomical marker used in identification of human-associated Staphylococcus succinus subsp. casei
PublicationThe aim of our study was to assess the sequencing of unique nucH gene fragment based on performed bioinformatics analysis as a novel diagnostic method for the identification of difficult to identify staphylococcal human pathogenic strains. Initially, PCR-RFLP-rrn analysis specific to the spacers between 16SrDNA and 23SrDNA followed by HhaI restriction analysis was performed. Further, sequencing of nucH and 16S rDNA genes fragments...
-
IN VITRO PROPAGATION OF RHODODENDRON TOMENTOSUM - AN ENDANGERED ESSENTIAL OIL BEARING PLANT FROM PEATLAND
PublicationRhododendron tomentosum Harmaja (formerly Ledum palustre L.) is a medicinal peat bog plant native to northern Europe, Asia and North America. This plant has a distinctive aroma thanks to the presence of essential oil, to which it also owes its anti-inflammatory, analgesic, antimicrobial and insecticidal properties. However, in Europe R. tomentosum is classified as an endangered species, mainly due to degradation of peatlands. In...
-
Mitotic catastrophe followed by necrosis versus pro-survival autophagy - two opposite cellular effects triggered upon C-1311 treatment in MCF-7 cells
PublicationPrzeprowadzone badania miały na celu określenie zdolności imidazoakrydonu C-1311 do indukcji katastrofy mitotycznej, nekrozy i autofagii w komórkach ludzkiego raka piersi MCF-7. Wszystkie eksperymenty przeprowadzone zostały przy stężeniu hamującym proliferację komórek nowotworowych w 80%. Wykazano, że pochodna C-1311 powoduje przejściową akumulację komórek MCF-7 w fazie G2 cyklu życiowego (59% po 48 godz. inkubacji) z równoczesnym...
-
Numerical modeling of wave-seabed-structure interaction.
PublicationPraca doktorska przedstawia propozycję nowoczesnego rozwiązania obciążeń hydraulicznych przy uwzględnieniu nieliniowych oddziaływań pomiędzy falą wodną, przepuszczalnym dnem i porowatą konstrukcją morską. Nabrzeże pionowe, falochron podwodny i falochron kompozytowy były obiektem eksperymentów numerycznych, których wyniki były weryfikowane przy użyciu wyników laboratoryjnych (niszczących i nieniszczących). Krótki przegląd bieżącej...
-
Mechanism of recognition of parallel G-quadruplexes by DEAH/RHAU helicase DHX36 explored by molecular dynamics simulations
PublicationBecause of high stability and slow unfolding rates of G-quadruplexes (G4), cells have evolved specialized helicases that disrupt these non-canonical DNA and RNA structures in an ATP-dependent manner. One example is DHX36, a DEAH-box helicase, which participates in gene expression and replication by recognizing and unwinding parallel G4s. Here, we studied the molecular basis for the high affinity and specificity of DHX36 for parallel-type...
-
Sposób pozyskiwania energii fal morskich lub oceanicznych
InventionsPrzedmiotem wynalazku jest sposób pozyskiwania energii fal morskich lub oceanicznych, mający zastosowanie zwłaszcza w akwenach przybrzeżnych i zagrożonych niszczeniem nadbrzeży przez fale morskie. W jednym metrze fali o wysokości 1,80 m i długości 38 m uwięziona jest moc wynosząca 10 kW. Przyczynami falowania mogą być tarcia wiatru o powierzchnię wody, albo zmiany ciśnienia atmosferycznego na powierzchni oceanu, wywołane...
-
Retained features of embryonic metabolism in the adult MRL mouse
PublicationThe MRL mouse is an inbred laboratory strain that was derived by selective breeding in 1960 from the rapidly growing LG/J (Large) strain. MRL mice grow to nearly twice the size of other commonly used mouse strains, display uncommonly robust healing and regeneration properties, and express later onset autoimmune traits similar to Systemic Lupus Erythematosis. The regeneration trait (heal) in the MRL mouse maps to 14-20 quantitative...
-
A new cold-adapted beta-D-galactosidase from the Antarctic Arthrobacter sp. 32c - gene cloning, overexpression, purification and properties
PublicationThe development of a new cold-active β-D-galactosidases and microorganisms that efficiently ferment lactose is of high biotechnological interest, particularly for lactose removal in milk and dairy products at low temperatures and for cheese whey bioremediation processes with simultaneous bio-ethanol production. In this article, we present a new β-D-galactosidase as a candidate to be applied in the above mentioned biotechnological...
-
A Concept of Using Knowledge Models to Secure Intellectual Capital in Enterprise
PublicationWithout a doubt, knowledge has become the most important resource for all companies in the world. Knowledge extended of our own observations and practice is being called experience. With the increase of knowledge and gained experience, the individual becomes more and more valuable employee in terms of business. The above mentioned knowledge can be gained only by self-improvement, which requires time and other necessary elements...
-
Folate/homocysteine metabolism and lung cancer risk among smokers
PublicationBackground: Folate and homocysteine are involved in DNA synthesis and methylation processes, which are deregulated during carcinogenesis. Objectives: The aim of this study was to assess the relationship between folate/homocysteine concentrations, the functional polymorphisms of folate/homocysteine genes and lung cancer risk among cigarette smokers. Study design: The study included 132 lung cancer patients and 396 controls from...
-
Smart Knowledge Engineering for Cognitive Systems: A Brief Overview
PublicationCognition in computer sciences refers to the ability of a system to learn at scale, reason with purpose, and naturally interact with humans and other smart systems, such as humans do. To enhance intelligence, as well as to introduce cognitive functions into machines, recent studies have brought humans into the loop, turning the system into a human–AI hybrid. To effectively integrate and manipulate hybrid knowledge, suitable technologies...
-
Genetic and biochemical characterization of yeasts isolated from Antarctic soil samples
PublicationThe Polish Arctowski Station is situated in the maritime Antarctic on the western shore ot' Admiralty Bay and encompasses terrestrial habitats which are not perma-nently covered by ice, in contrast to morę than 90% of the island's surface area. Over the past several decades, stud-ies exploring the soils of those habitats have revealed a considerable diversity of bacteria, filamentous fungi, and, to a lesser extent, yeasts; however,...
-
Organizmy obecne w osadzie dennym basenów kąpielowych
PublicationMonitorowanie parametrów wody basenowej jest istotne z uwagi na zdrowie osób korzystających z basenu jak i pracowników obsługi. Istnieją trzy źródła materii organicznej: woda dostarczona do basenu, pasywne zanieczyszczenia wnoszone przez osoby kąpiące się oraz zanieczyszczenia organiczne w postaci moczu i kału. Jakość wody w basenach powinna być porównywalna do jakości wody pitnej. Jakość mikrobiologiczna opiera się na tradycyjnych...
-
Towards an experience based collective computational intelligence for manufacturing
PublicationKnowledge based support can play a vital role not only in the new fast emerging information and communication technology based industry, but also in traditional manufacturing. In this regard, several domain specific research endeavors have taken place in the past with limited success. Thus, there is a need to develop a flexible domain independent mechanism to capture, store, reuse, and share manufacturing knowledge. Consequently,...
-
Molecular basis and quantitative assessment of TRF1 and TRF2 protein interactions with TIN2 and Apollo peptides
PublicationShelterin is a six-protein complex (TRF1, TRF2, POT1, RAP1, TIN2, and TPP1) that also functions in smaller subsets in regulation and protection of human telomeres. Two closely related proteins, TRF1 and TRF2, make high-affinity contact directly with double-stranded telomeric DNA and serve as a molecular platform. Protein TIN2 binds to TRF1 and TRF2 dimer-forming domains, whereas Apollo makes interaction only with TRF2. To elucidate...
-
A cold-adapted esterase from psychrotrophic Pseudoalteromonas sp. strain 643A
PublicationDzięki zastosowaniu lipaz możliwa jest biotechnologiczna produkcja m.in. biodegradowalnych biopolimerów, biopaliw (tzw. biodiesel), wielu terapeutyków, herbicydów, pestycydów, kosmetyków, substancji smakowych, serów i in. Szczególnie interesującą grupę stanowią enzymy lipolityczne, pochodzące z organizmów psychrofilnych i psychrotrofowych, ze względu na zdolność do wydajnego katalizowania reakcji w temperaturach rzędu 10-20 ºC....
-
B-GALACTOSIDASE ARTHROBACTER SP. 32cB - OBTAINING THE GENE SEQUENCE, CONSTRUCTION OF THE EXPRESSION SYSTEM, BIOSYNTHESIS AND BIOCHEMICAL CHARACTERIZATION OF THE ENZYME
PublicationINTRODUCTION: β-Galactosidase is an enzyme which catalyzes the hydrolysis of O glycosidic bond in β-galactosides. Another activity of β galactosidase is a transglycosylation activity. The main industrial use of this protein is the hydrolysis of lactose in milk in a cooling conditions. Synthesis of galactooligosaccharides, which are mostly used as a prebiotics added to some foods or available as dietary supplements, is only one...
-
THE GENE CLONING, OVEREXPRESSION, PURIFICATION AND BIOCHEMICAL CHARACTERISATION OF A NEW COLD-ADAPTED β–GALACTOSIDASE FROM ARTHROBACTER SP. VII-4
PublicationINTRODUCTION. β-Galactosidase [EC 3.2.1.23] is an enzyme that catalyzes the hydrolysis of O-glycosidic linkages in galactosides. It is commercially used in dairy industry for the production of milk with reduced lactose content. Potentially, the best method for lactose removal under cooling conditions should be carried out with a cold-adapted enzyme. AIM. The aim of this study was to determine the taxonomic affiliation of the isolate...
-
Activation of endogenous regenerative potential in mammals using epigenetic inhibitor zebularine
PublicationIn this work, a nucleoside inhibitor of DNA methyltransferases, zebularine, was investigated as a wound healing and regeneration promoting agent. It was found that a high dose of intraperitoneally delivered zebularine improved punch wound closure in the ear pinna in mice. Both cytidine, a zebularine analogue, and uridine, a zebularine metabolite, did not promote ear pinna hole closure. The effect of zebularine on ear pinna healing...
-
Kwasy nukleinowe w żywności występowanie i właściwości reologiczne
PublicationW naukach o żywieniu kwasy nukleinowe uznawane były dotychczas za mało istotny składnik żywności. Ich właściwości odżywcze nie są uwzględniane przy formułowaniu zaleceń żywieniowych, mimo że należą do podstawowych składników żywności, zwłaszcza surowej lub nisko przetworzonej. Są one obecne głównie w szybko rosnących komórkach tkanek oraz tych, które zachowały zdolność do wzrostu i regeneracji. W niniejszej pracy przedstawiono...
-
Molecular identification and genotyping of Staphylococci: genus, species, strains, colnes, lineages, and interspecies exchanges
PublicationStaphylococci are increasingly recognized as etiological agents of many opportunistic human and animal infections, emphasizing the need for a rapid and accurate identification, even to a genotypical level of these bacteria. In the recent years, there has been a significant progress in typing and phylogenetic study of Staphylococcus species. Here, we describe molecular methods used in taxonomy as well as staphylococci characterization....
-
Effect of neoteric solvents on the activated sewage sludge activity
PublicationCiecze jonowe (ILs) cieszą sie rosnącym zainteresowaniem z uwagi na fakt, iż znajdują coraz więcej zastosowań jako alternatywa dla rozpuszczalników organicznych. W związku z zaistnieniem możliwości zastosowania ILs w nowych czystych technologiach nastała potrzeba zbadania ich toksyczności w stosunku do organizmów żywych i środowiska. Celem badań było oszacowanie wpływu cieczy jonowych na wyselekcjonowane mikroorganizmy osadu czynnego....
-
2-Methoxyestradiol and Hydrogen Peroxide as Promising Biomarkers in Parkinson’s Disease
PublicationEstrogens function in numerous physiological processes including controlling brain cell growth and differentiation. 2-Meth- oxestradiol (2-ME2), a 17β-estradiol (E2) metabolite, is known for its anticancer effects as observed both in vivo and in vitro. 2-ME2 affects all actively dividing cells, including neurons. The study aimed to determine whether 2-ME2 is a potentially cancer-protective or rather neurodegenerative agent in a...
-
Anticancer Study on IrIII and RhIII Half-Sandwich Complexes with the Bipyridylsulfonamide Ligand
PublicationTwo non-platinum compounds, organometallic half-sandwich complexes [(η 5 -Cp)IrCl(L)]PF 6 (1) and [(η 5 -Cp)RhCl(L)]PF 6 (2) were prepared by treating pentamethylcyclopentadienyl chloride dimers of iridium(III) or rhodium(III) with the obtained 4-amino-N-(2,2'-bipyridin-5- yl)benzenesulfonamide ligand (L) and ammonium hexafluorophosphate. The crystal structures of ligand (L) and complexes 1 and 2 were obtained and then analyzed. Coordination...
-
Host and pathogen factors in Klebsiella pneumoniae upper urinary tract infections in renal transplant patients
PublicationPurpose . To analyse the role of virulence factors (VFs) and host in Klebsiella pneumoniae upper urinary tract infections (UTIs) in renal transplant (RTx) recipients. Methodology. Clinical and demographic data were registered prospectively. Phylogenetic background of K. pneumoniae isolates was analysed by PCR melting profiles (MP) and the following VFs genes: fimH-1, uge, kpn, ycfM, mrkD, rmpA, magA, hlyA, cnf-1, irp-1, irp-2,...
-
Anticancer imidazoacridinone C-1311 inhibits hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and angiogenesis
PublicationAntitumor imidazoacridinone C-1311 is a DNA-reactive topoisomerase II and FLT3 receptor tyrosine kinase inhibitor. Here, we demonstrate the mechanism of C-1311 inhibitory action on novel targets: hypoxia-inducible factor-1α (HIF-1α), vascular-endothelial growth factor (VEGF), and angiogenesis. In a cell-free system, C-1311 prevented HIF-1α binding to an oligonucleotide encompassing a canonical hypoxia-responsive element (HRE),...
-
Targeting of FLT3-ITD kinase contributes to high selectivity of imidazoacridinone C-1311 against FLT3-activated leukemia cells
PublicationDrugs targeting receptor tyrosine kinase FLT3 are of particular interest since activating FLT3-internal tandem duplication (ITD) mutations abundantly occur in fatal acute myeloid leukemias (AMLs). Imidazoacridinone C-1311, a DNA-reactive inhibitor of topoisomerase II, has been previously shown to be a potent and selective inhibitor of recombinant FLT3. Here, we expand those findings by studying its effect on leukemia cells with...
-
New digital aids for pateints after laryngectomy
PublicationPowrót do prawidłowej komunikacji z otoczeniem pacjentów po laryngektomii jest możliwy poprzez wykształcenie zastępczej mowy przełykowej lub gardłowej a w pozostałych przypadkach, kiedy się to nie udaje, poprzez zastosowanie elektronicznych protez (wibratorów szyjnych) wprowadzających w drgania tkanki dna jamy ustnej i szyi. Nawet, gdy dochodzi do wykształcenia mowy zastępczej, jakość mowy artykułowanej przełykowo jak i artykułowanej...
-
5-Thiocyanato-2′-deoxyuridine as a possible radiosensitizer: electron-induced formation of uracil-C5-thiyl radical and its dimerization
PublicationIn this work, we have synthesized 5-thiocyanato-2′-deoxyuridine (SCNdU) along with the C6-deuterated nucleobase 5-thiocyanatouracil (6-D-SCNU) and studied their reactions with radiation-produced electrons. ESR spectra in γ-irradiated nitrogen-saturated frozen homogeneous solutions (7.5 M LiCl in H2O or D2O) of these compounds show that electron-induced S–CN bond cleavage occurs to form a thiyl radical (dU-5-S˙ or 6-D-U-5-S˙) and...
-
Laboratorium Syntezy Innowacyjnych Materiałów i Elementów
LaboratoriesZespół specjalistycznych urządzeń pozwala dokonywać syntezy diamentu mikro- i nanokrystalicznego oraz diamentu domieszkowanego borem i azotem do zastosowań w optoelektronice oraz nanosensoryce. Domieszkowany borem nanodiament (BDD) jest obecnie najwydajniejszym materiałem półprzewodnikowym do zastosowania w wytwarzaniu biosensorów elektrochemicznych. Laboratorium może otrzymywać ciągłe cienkie polikrystaliczne, domieszkowane elektrody...
-
Techniki amplifikacji kwasów nukleinowych -2022-2023
e-Learning CoursesSpecjalność: Biotechnologia molekularna (WCh), II stopnia, stacjonarne Prowadzący wykład: dr hab. Beata Krawczyk, prof. uczelni - forma stacjonarna Prowadzący laboratoria: mgr inż. Bartosz Ostrowski - zajęcia stacjonarne na PG, sala 17 WCH C. Kontakt: (58) 347-23-83 Beata Krawczyk (wykłady): e-mail: beata.krawczyk@pg.edu.pl; WCH B; p.218; Bartosz Ostrowski, WCH B, p.218 tel. (58) 347-13-83 Konsultacje: stacjonarnie we wtorki ...
-
Techniki amplifikacji kwasów nukleinowych -2021-2022
e-Learning CoursesSpecjalność: Biotechnologia molekularna (WCh), II stopnia, stacjonarne Prowadzący wykład: dr hab. Beata Krawczyk, prof. uczelni - stacjonarnie na uczelni Prowadzący laboratoria: mgr inż. Magdalena Fordon - zajęcia stacjonarne na PG, sala 17 WCH C. Kontakt: (58) 347-23-83 Beata Krawczyk (wykłady): e-mail: beata.krawczyk@pg.edu.pl; WCH B; p.218; Magdalena Fordon (laboratoria): magda.fordon@gmail.com; WCH B; p.218 Konsultacje:...
-
Reakcje izotiocyjanianów z heterocyklicznymi aminami aromatycznymi i ich rola w inaktywacji kancerogenów w żywności
PublicationWyniki licznych badań epidemiologicznych wskazują na pozytywny wpływ diety bogatej w warzywa na zdrowie człowieka. Szczególne znaczenie roślin z rodziny kapustowatych, a wśród nich najważniejszej dietetycznie kapusty, wiąże się z obecnością w tych warzywach biologicznie aktywnych składników: glukozynolanów i produktów ich rozpadu - związków indolowych i izotiocyjanianów. Na zainteresowanie zasługują izotiocyjaniany ze względu na...
-
Bacteriophages as potential tools for use in antimicrobial therapy and vaccine development
PublicationThe constantly growing number of people suffering from bacterial, viral, or fungal infections, parasitic diseases, and cancers prompts the search for innovative methods of disease prevention and treatment, especially based on vaccines and targeted therapy. An additional problem is the global threat to humanity resulting from the increasing resistance of bacteria to commonly used antibiotics. Conventional vaccines based on bacteria...
-
Genotyping and characterization of virulence factors in Escherichia coli strains isolated from patients from Polish hospital
PublicationZakażenia układu moczowego (UTIs - ang. urinary track infections) są jedną z najczęstszy infekcji, za które w 85% przypadków odpowiedzialne są bakterie Escherichia coli. Infekcje układu moczowego mogą prowadzić do groźnych powikłań m. in. odmiedniczkowego zapalenia nerek. Śmiertelność wśród tej grupy pacjentów wynosi 1-3%. Zakażenia układu moczowego występują również u ok. 7% kobiet ciężarnych. W pracy przebadano 82 szczepy E....
-
Electrochemistry meets enzymes: Investigation of the biotransformation pathway of C-1311 based on electrochemical simulation in comparison to in vitro methods.
PublicationThe knowledge of the metabolic pathways and the biotransformation of new drugs is one of the major challenges in pharmaceutical research. It is crucial for elucidation of degradation routes of the new biologically active compounds, especially in the area of possible toxicity. Conventional in vitro drug metabolism studies are based on incubating drug candidate with e.g. hepatocytes or, most importantly, liver cell microsomes and...
-
Caffeine Inhibits Differentiation Of Lung Cancer Stem Cells By Modulating Their Respiratory Metabolism
PublicationIt is ell established that many tumor types contain a fraction of cells, with stem cell-like properties, called cancer stem cells (CSCs), that are resistant to apoptosis induced by therapeutic agents. The presence of CSCs may explain why a standard anticancer treatment, that eliminates only differentiated cancer cells, does not lead to cancer cure.We previously showed the existence of caffeine-sensitive mechanism that controls...
-
Zużyty płyn wiertniczy - odpad czy ściek?
PublicationPłuczka wiertnicza wywiera znaczący wpływ na wszystkie operacje podczas procesu technologicznego jakim jest wiercenie górotworu. Odpowiedni skład płuczki wpływa na ilość jak i jakość pozyskiwania ropy naftowej i gazu ziemnego, dlatego powinien spełniać zadania mające związek z jego określonymi właściwościami. Jednym z najważniejszych zadań płuczki jest oczyszczanie dna otworu ze zwiercin, a następnie ich transport na powierzchnie...
-
Siderofory bakteryjne – rola w patogenezie i potencjał wykorzystania w diagnostyce
PublicationDo prawidłowego funkcjonowania i wzrostu organizmów żywych zarówno ludzi, jak i bakterii niezbędne jest żelazo. Mikroelement ten występuje na dwóch różnych stopniach utlenienia: jako jon żelazawy (Fe2+) rozpuszczalny w roztworach fizjolo-gicznych oraz jako jon żelazowy (Fe3+), który nie tworzy roztworów wodnych [1]. Dzięki swoim właściwościom oksydo-redukcyjnym, żelazo wpływa na poprawne funkcjonowanie bardzo licznej grupy enzymów,...
-
Rozdział 5. Nowoczesne metody diagnostyki zakażeń grzybiczych wywoływanych przez dermatofity = Novel diagnostic methods of funfal infections caused by dermatophytes
PublicationDermatofity są głównym czynnikiem etiologicznym grzybic powierzchownych. Do tej klasy grzybów należą przedstawiciele trzech rodzajów: Epidermophyton, Microsporum i Trichophyton. Klasyczne metody diagnostyczne polegają na bezpośredniej ocenie mikroskopowej oraz badaniu hodowlanym. Identyfikacja gatunkowa jest możliwa na podstawie cech makroskopowych kolonii: ich struktury, koloru, kształtu, oraz obserwacji mikroskopowych (kształt...
-
Total cross-section measurements for electron collision with α-tetrahydrofurfuryl alcohol (C5H10O2)
PublicationPrzedstawiono całkowite przekroje czynne (TCS) na rozpraszanie elektronów na drobinach α-tetrahydrofurfurylu, zmierzone w zakresie energii zderzenia 1-370 eV oraz policzone powyżej 50 eV. Przedyskutowano otrzymaną zależność TCS od energii. Wyniki porównano z dostępnymi teoretycznymi i eksperymentalnymi przekrojami czynnymi.
-
Absolute total cross-section measurements for electron collision with tetrahydrofuran
PublicationZmierzono absolutny, całkowity przekrój czynny na rozpraszanie elektronów na drobinach tetrahydrofuranu (C4H8O) w zakresie energii zderzenia 1-370 eV. Otrzymane wyniki pozostają w dobrej zgodności z sumą obliczonych przekrojów elestycznych i jonizacyjnych. Przeprowadzono porównananie z innymi dostępnymi przekrojami czynnymi.
-
Identyfikacja sekwencji markerowych w mikologicznej diagnostyce molekularnej
PublicationProcedury diagnostyki mikologicznej coraz częściej wykorzystują metody molekularne, oparte na analizie kwasów nukleinowych. Pozwala to na skrócenie czasu analizy oraz polepszenie wielu innych, istotnych dla procesu diagnostycznego parametrów takich jak: czułość, powtarzalność, specyficzność oraz potencjał różnicujący. Istotnym etapem przy projektowaniu układów do diagnostyki molekularnej grzybów jest wybór celu molekularnego. Powszechnie...
-
Guanosine Dianions Hydrated by One to Four Water Molecules
PublicationIntermolecular interactions such as those present in molecule···water complexes may profoundly influence the physicochemical properties of molecules. Here, we carried out an experimental–computational study on doubly deprotonated guanosine monophosphate···water clusters, [dGMP – 2H]2–·nH2O (n = 1–4), using a combination of negative anion photoelectron spectroscopy (NIPES) with molecular dynamics (MD) and quantum chemical (QM) calculations....