Wyszukiwarka
Filtry
wszystkich: 3315
wybranych: 2772
-
Katalog
- Publikacje 2772 wyników po odfiltrowaniu
- Czasopisma 28 wyników po odfiltrowaniu
- Osoby 67 wyników po odfiltrowaniu
- Projekty 1 wyników po odfiltrowaniu
- Laboratoria 2 wyników po odfiltrowaniu
- Kursy Online 18 wyników po odfiltrowaniu
- Wydarzenia 5 wyników po odfiltrowaniu
- Dane Badawcze 422 wyników po odfiltrowaniu
Filtry wybranego katalogu
wyświetlamy 1000 najlepszych wyników Pomoc
Wyniki wyszukiwania dla: folded proteins
-
The Unfolded Protein Response: A Double-Edged Sword for Brain Health
PublikacjaEfficient brain function requires as much as 20% of the total oxygen intake to support normal neuronal cell function. This level of oxygen usage, however, leads to the generation of free radicals, and thus can lead to oxidative stress and potentially to age-related cognitive decay and even neurodegenerative diseases. The regulation of this system requires a complex monitoring network to maintain proper oxygen homeostasis. Furthermore,...
-
Function and Frustration of Multi-Drug ABC Exporter Protein and Design of Model Proteins for Drug Delivery Using Protein Hydration Thermodynamics
Publikacja -
Elastic-contractile model proteins: Physical chemistry, protein function and drug design and delivery
Publikacja -
How proteins bind to DNA: target discrimination and dynamic sequence search by the telomeric protein TRF1
PublikacjaTarget search as performed by DNA-binding proteins is a complex process, in which multiple factors contribute to both thermodynamic discrimination of the target sequence from overwhelmingly abundant off-target sites and kinetic acceleration of dynamic sequence interrogation. TRF1, the protein that binds to telomeric tandem repeats, faces an intriguing variant of the search problem where target sites are clustered within short fragments...
-
Influence of annealing on joint zone of explosive welded and rolled Al-Cuplate
PublikacjaRosnące wciąż wymagania co do metalowych materiałów jednorodnych przyczyniło się do zwiększenia zainteresowania materiałami platerowanymi. Blachy platerowane często znajdują zastosowanie w przemyśle chemicznym i petrochemicznym jako materiały antykorozyjne odporne na działanie agresywnego środowiska, w przemyśle okrętowym jako łączniki stopów aluminium ze stalą, w budowie zgrzewarek jako połączenia szczęk z blachą stalową, w przemyśle...
-
Chitosan-protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials.
PublikacjaAIMS: The development of technology for preparing chitosan-protein scaffolds loaded with lysostaphin, which potentially could be used as dressing for wound treatment and soft tissue infections caused by Staphylococcus aureus. METHODS AND RESULTS: The unique technology of chitosan solubilization using gaseous CO(2) instead of organic or inorganic acids was used for the incorporation of lysostaphin, the enzyme that exhibits bactericidal...
-
Problems of analysis of axially loaded steel behavior under laser welded panel
PublikacjaWprowadzanie do praktyki nowych rozwiązań konstrukcyjnych wymaga posiadania danych wejściowych do procesu modelowania ich zachowania. Takim nowym elementem jest stalowy, spawany laserowo panel typu sandwich. W pracy pokazano wy-brane problemy towarzyszące modelowaniu zachowań takiej struktury. Pokazano podstawy teoretyczne wyznaczania sztyw-ności panelu a następnie omówiono wyniki badań wyboczenio-wych serii modeli o zmiennej geometrii.
-
Extension of the Unres Package for Physics-Based Coarse-Grained Simulations of Proteins and Protein Complexes to Very Large Systems
Publikacja -
Proteins
PublikacjaStruktura białek wpływa na ich wartość odżywczą, właściwości funkcjonalne wżywności oraz reakcje w surowcach i produktach żywnościowych. Warunki prze-chowywania oraz obróbki w przemyśle i w czasie przygotowywania kulinarnegowywołują przemiany białek istotne dla jakości żywności. Przez celowe, enzy-matyczne i chemiczne modyfikowanie składu i struktury białek można wpływaćna ich właściwości funkcjonalne w żywności i wartość...
-
Mechanism of Osmolyte Stabilization–Destabilization of Proteins: Experimental Evidence
PublikacjaIn this work, we investigated the influence of stabilizing (N,N,N-trimethylglycine) and destabilizing (urea) osmolytes on the hydration spheres of biomacromolecules in folded forms (trpzip-1 peptide and hen egg white lysozyme─hewl) and unfolded protein models (glycine─GLY and N-methylglycine─NMG) by means of infrared spectroscopy. GLY and NMG were clearly limited as minimal models for unfolded proteins and should be treated with...
-
Dimeric structure of the N-terminal domain of PriB protein from Thermoanaerobacter tengcongensis solved ab initio
PublikacjaPriB is one of the components of the bacterial primosome, which catalyzes the reactivation of stalled replication forks at sites of DNA damage. The N-terminal domain of the PriB protein from the thermophilic bacterium Thermoanaerobacter tengcongensis (TtePriB) was expressed and its crystal structure was solved at the atomic resolution of 1.09 Å by direct methods. The protein chain, which encompasses the first 104 residues of the...
-
Load-carrying capacity of axially loaded concrete-filled steel tubular columns made of thin tubes
PublikacjaAn experimental investigation was conducted on 30 CFST columns. An influence of the following factors on load-carrying capacity of the investigated columns was analyzed: the column slenderness (l1 = 42, l2 = 27 and l3 = 15), the tube thickness (the reinforcement ratio was equal to 4% or 6%), the way of applying the load to CFST columns (through the concrete core or through the entire cross-section), the bond strength between a...
-
The Role of Proteins in Food
PublikacjaThis chapter describes the effect of proteins on the sensory attributes and the biological value and safety of foods. The role of proteins depends on their amino acid composition and structure, on changes due to storage and processing, as well as on interactions with other food components. The effect on the sensory quality of foods is brought about by hydrophobicity, solubility, water holding capacity, gelling, film formation,...
-
Structural Changes and Their Implications in Foamed Flexible Polyurethane Composites Filled with Rapeseed Oil-Treated Ground Tire Rubber
PublikacjaThe utilization of post-consumer car tires is an essential issue from an ecological andeconomic point of view. One of the simplest and the least harmful methods is their material recyclingresulting in ground tire rubber (GTR), which can be further applied as fillers for polymer-basedcomposites. Nevertheless, insufficient interfacial interactions implicate the necessity of GTR modi-fication before introduction into polymer matrices....
-
Activity-based protein profiling guided identification of urine proteinase 3 activity in subclinical rejection after renal transplantation
Publikacja -
Conservation and diversity of MutS proteins
PublikacjaThe homologues of MutS, mismatch repair protein, exist in all prokaryotes, with the exception of Actinobacteria, Mollicutes and part of the Archaea. Multiple alignments of 316 MutS amino acid sequences from 169 species revealed conserved residues and sequence motifs distinguishing MutS homologues. All MutS homologues show high conservation within the ATPase domain. MutS1, the homologue responsible for DNA mismatch recognition,...
-
Conserved motifs of MutL proteins
PublikacjatThe MutL protein is best known for its function in DNA mismatch repair (MMR). However, there isevidence to suggest that MutL is not only the linker connecting the functions of MutS and MutH in MMR,but that it also participates in other repair systems, such as Very Short Patch (VSP), Base Excision (BER)and Nucleotide Excision Repair (NER). This study set out to identify the most highly conserved aminoacid sequence motifs in MutL...
-
Concentration of proteins in the whey stream
PublikacjaZastosowano metodę separacji pianowej do odzyskiwania białka z serwatki. Wykazano silny wpływ prędkości powietrza w kolumnie, początkowego stężenia białka w serwatce i pH roztworu na przebieg i skuteczność flotacji. Współczynnik wzbogacenia maleje ze wzrostem prędkości przepływu powietrza oraz ze wzrostem początkowego stężenia białka serwatkowego. Najwyższą wartość współczynnika wzbogacenia uzyskano dla pH naturalnej serwatki.
-
Design of waveguide filters using multiple irises and folded elements.
PublikacjaW pracy został zaprezentowany przegląd metod projektowania filtrów falowodowych w kontekście zarówno klasycznych metod syntezy jak i współczesnych algorytmów. W szczególności rozważonoanalizę filtrów z wieloaperturowymi przegrodami oraz filtrówzbudowanych w wygiętych odcinkach falowodów. Zaprezentowano przykłady w pełni wykorzystujące zalety wygiętych falowodów a także pokazano wydajne metody optymalizacji oparte na badaniu położeń...
-
Influence of nodes with positive eccentricity on load-carrying capasity of truss' top chords in trusses made of welded cold-formed members.
PublikacjaIn order to establish the influence of eccentric crossing of truss members on the load-carrying capacity of the folded chord of a hat-section truss, a research model was created and preliminary numerical analysis of the truss was performed with the use of Marc Mentat programme. Distorial instability of crossheads caused by deviation of loose (unmounted) channel section web’s bends was observed. Due to the slenderness of the section’s...
-
Miniaturized uniplanar triple-band slot dipole antenna with folded radiator
PublikacjaA miniaturized uniplanar slot dipole for triple-frequency operation is presented. The antenna consists of a folded slot radiator with an increased number of degrees of freedom that allow for efficient size reduction. Rigorous electromagnetic (EM)-driven design optimization is applied in order to achieve the smallest possible size while maintaining acceptable levels of antenna reflection at the required operating frequencies. The...
-
UNRES-Dock—protein–protein and peptide–protein docking by coarse-grained replica-exchange MD simulations
Publikacja -
Molecular Dynamics Studies on Amyloidogenic Proteins
Publikacja -
Molecular Dynamics Studies on Amyloidogenic Proteins
Publikacja -
Antioxidants and proteins in ethylene-treated kiwifruits
PublikacjaW pracy porównano właściwości przeciwutleniające i profil białek owoców kiwi poddanych działaniu etylenu i powietrza. Wysokie współczynniki korelacji między całkowitą zawartością fenoli i właściwościami przeciwutleniającymi uzyskano dla trzech metod określania właściwości przeciwutleniających: ABTS, TEAC i CUPRAC. W paśmie białek owoców kiwi rozdzielonych za pomocą elektroforezy stwierdzono różnice w paśmie siarczanu dodecylu sodu...
-
The role of proteins in food. Chapter 6
PublikacjaStruktura i konformacja wpływają na biologiczne oraz funkcjonalne właściwości białek i na ich rolę w tworzeniu pożądanych cech produktów żywnościowych. Właściwości funkcjonalne to rozpuszczalność w środowisku wodnym o różnej sile jonowej, wodochłonność oraz zdolność żelowania, tworzenia błon, emulgowania lipidów i pienienia się. Białka mięsa, ryb, mleka, jaj, nasion roślin strączkowych, nasion zbóż oraz drobnoustrojów różnią się...
-
New plasma preparation approach to enrich metabolome coverage in untargeted metabolomics: plasma protein bound hydrophobic metabolite release with proteinase K
Publikacja -
Crystal structures of the DsbG disulfide isomerase reveal an unstable disulfide
PublikacjaDsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. Here, we present two crystal structures of DsbG at 1.7- and 2.0-Angstrom resolution that are meant to represent the reduced and oxidized forms, respectively. The oxidized structure, however, reveals a mixture...
-
The Protein Folding Problem
Publikacja -
The measurement of conformational stability of proteins adsorbed on siloxanes
Publikacja -
Molecular basis of TRF proteins and their interactions with peptides
Publikacja -
1.21 Computation of Structure, Dynamics, and Thermodynamics of Proteins
Publikacja -
Coarse-Grained Models of Proteins: Theory and Applications
Publikacja -
Computational techniques for efficient conformational sampling of proteins
Publikacja -
On the Origin of Protein Superfamilies and Superfolds
PublikacjaDistributions of protein families and folds in genomes are highly skewed, having a small number of prevalent superfamiles/superfolds and a large number of families/folds of a small size. Why are the distributions of protein families and folds skewed? Why are there only a limited number of protein families? Here, we employ an information theoretic approach to investigate the protein sequence-structure relationship that leads to...
-
Influence of Osmolytes on Protein and Water Structure: A Step To Understanding the Mechanism of Protein Stabilization
PublikacjaResults concerning the thermostability of hen egg white lysozyme in aqueous solutions with stabilizing osmolytes, trimethylamine-N-oxide (TMAO), glycine (Gly), and its N-methyl derivatives, N-methylglycine (NMG), N,N-dimethylglycine (DMG), and N,N,N-trimethylglycine (betaine, TMG), have been presented. The combination of spectroscopic (IR) and calorimetric (DSC) data allowed us to establish a link between osmolytes’ influence on...
-
Surface layer defects on injection-molded plastic components
PublikacjaProces wtryskiwania jakkolwiek jest powszechnie stosowany w przemyśle nie jest jeszcze do końca przebadany. W pracy przedstawiono model i symulację numeryczna procesu wtryskiwania wykonaną na bazie programu FIDAP. Na tej podstawie przedstawiono proces zachowania się tworzywa podczas procesu wtryskiwania w strefie przyściennej formy. Wyniki z symulacji porównano z danymi ekspermentalnymi.
-
The construction of bifunctional fusion proteins consisting of MutS and GFP
PublikacjaSkonstruowano białka chimeryczne zawierające białko MutS z Thermus thermophilus i białko zielonej fluoresceiny GFP z Aequorea victoria, posiadające dla łatwiejszego oczyszczania domeny oligo-histydynowe na N- lub C-końcu. Fuzyjne białka rozpoznawały niekomplementarności w DNA podobnie do MutS T. thermophilus. Fluorescencyjne białka rozpoznające niekomplementarne DNA mogą być użyteczne w wykrywaniu jednonukleotydowych polimorfizmów...
-
The construction of bifunctional fusion proteins consisting of MutS and GFP
Publikacja -
Molecular Recognition in Complexes of TRF Proteins with Telomeric DNA
PublikacjaTelomeres are specialized nucleoprotein assemblies that protect the ends of linear chromosomes. In humans and many other species, telomeres consist of tandem TTAGGG repeats bound by a protein complex known as shelterin that remodels telomeric DNA into a protective loop structure and regulates telomere homeostasis. Shelterin recognizes telomeric repeats through its two major components known as Telomere Repeat-Binding Factors, TRF1...
-
Denaturation of proteins by surfactants studied by the Taylor dispersion analysis
Publikacja -
Dynamics of Chains as a Tool to Study Thermomechanical Properties of Proteins.
PublikacjaPolymer dynamics can be formulated on different levels of detail. One approach eliminates microscopic degrees of freedom and a polymer molecule is represented by a simplified structure—a chain. In the simplest case monomers of ideal chain have fixed length, and their orientation is independent of the orientations and positions of neighbouring monomers. This is reason that two monomers can co-exist at the same place. Ideal chain...
-
Recent Developments in Data-Assisted Modeling of Flexible Proteins
PublikacjaMany proteins can fold into well-defined conformations. However, intrinsically-disordered proteins (IDPs) do not possess a defined structure. Moreover, folded multi-domain proteins often digress into alternative conformations. Collectively, the conformational dynamics enables these proteins to fulfill specific functions. Thus, most experimental observables are averaged over the conformations that constitute an ensemble. In this...
-
Stabilizing agents and protein stability
PublikacjaBiałka są kluczowymi elementami żywych organizmów, jak również efektywnymi katalizatorami w biotechnologii i medycynie. Szybki rozwój wielu gałęzi biotechnologii wiąże się z coraz większym zapotrzebowaniem na produkty białkowe charakteryzujące się wysoką stabilnością. Jednym ze sposobów osiągnięcia większej stabilności białek jest wykorzystanie małych związków organicznych, tzw. osmolitów. Do tej grupy związków należą aminokwasy,...
-
Colloidal aspects of protein digestion
Publikacja.
-
Alternative and New Protein Sources
Publikacja -
Taurine as a water structure breaker and protein stabilizer
PublikacjaThe enhancing effect on the water structure has been confirmed for most of the osmolytes exhibiting both stabilizing and destabilizing properties in regard to proteins. The presented work concerns osmolytes, which should be classified as “structure breaking” solutes: taurine and N,N,N-trimethyltaurine (TMT). Here, we combine FTIR spectroscopy, DSC calorimetry and DFT calculations to gain an insight into the interactions between...
-
The effects of confinement in pores built of folded graphene sheets on the equilibrium of nitrogen monoxide dimerisation reaction
Publikacja -
The importance of the shape of the protein-water interface of a kinesin motor domain for dynamics of the surface atoms of the protein
PublikacjaSingle kinesin motor domain immersed in water has been investigated using molecular dynamics. It has been found that local properties of water in solvation shell change along with the nature of neighboring protein surface. However, a detailed analysis leads to the conclusion that the geometrical features of hydrogen bonds and overall structure of kinesin hydration water is not very different from bulk water. The local values of...
-
The importance of the shape of the protein–water interface of a kinesin motor domain for dynamics of the surface atoms of the protein
PublikacjaA single kinesin motor domain immersed in water has been investigated using molecular dynamics. It has been found that local properties of water in the solvation shell change along with the nature of the neighboring protein surface. However, a detailed analysis leads to the conclusion that the geometrical features of hydrogen bonds and overall structure of kinesin hydration water are not very different from bulk water. The local...
-
Endocrine responses following exhaustive strength exercise with and without the use of protein and protein-carbohydrate supplements
Publikacja -
ESCASA : Analytical estimation of atomic coordinates from coarse‐grained geometry for nuclear‐magnetic‐resonance ‐assisted protein structure modeling. I. Backbone and Hβ protons
PublikacjaA method for the estimation of coordinates of atoms in proteins from coarse-grained geometry by simple analytical formulas (ESCASA), for use in nuclear-magnetic-resonance (NMR) data-assisted coarse-grained simulations of proteins is proposed. In this paper, the formulas for the backbone Hα and amide (HN) protons, and the side-chain Hβ protons, given the Cα-trace, have been derived and parameterized, by using the interproton distances...
-
Zastosowanie addytywów w separacji pianowej białek serwatkowych i białek mlecznych
PublikacjaZaproponowano proces separacji pianowej do wydzielania białek ze strumieni poprodukcyjnych przemysłu mleczarskiego Ponadto zaproponowano zastosowanie w separacji pianowej białek serwatkowych i mlecznych addytywów dopuszczonych ustawą do stosowania w żywności. Jako cel pracy przyjęto uzyskanie wyników uzasadniających wybór takich addytywów, które intensyfikują przebieg i polepszają wynik procesu separacji pianowej białek. W części...
-
Fatty acyl benzamido antibacterials based on inhibition of DnaK-catalyzed protein folding
PublikacjaWe have reported that the hsp70 chaperone DnaK from Escherichia coli might assist protein folding by catalyzing the cis/trans isomerization of secondary amide peptide bonds in unfolded or partially folded proteins. In this study a series of fatty acylated benzamido inhibitors of the cis/trans isomerase activity of DnaK was developed and tested for antibacterial effects in E. coli MC4100 cells. Nα-[Tetradecanoyl-(4-aminomethylbenzoyl)]-l-asparagine...
-
Structure and function of natural proteins for water transport: general discussion
Publikacja -
Oxidative Modification of Proteins in Pediatric Cystic Fibrosis with Bacterial Infections
Publikacja -
Contribution of small heat shock proteins to muscle development and function
Publikacja -
Carotenoid binding to proteins: Modeling pigment transport to lipid membranes
Publikacja -
Immunogenic and protective activity of recombinant chimeric Toxoplasma gondii proteins
PublikacjaToxoplasmosis, one of the most common parasitic invasions worldwide, may pose a great threat for individuals with weakened immune system. The parasite also causes considerable economic losses due to infection of livestock. To date there is no efficient immunoprophylaxis for humans and animals, which would provide long-lasting protection against T. gondii invasion. Chimeric proteins, containing several selected fragments of parasite...
-
Core spliceosomal Sm proteins as constituents of cytoplasmic mRNPs in plants
Publikacja -
Core spliceosomal Sm proteins as constituents of cytoplasmic mRNPs in plants
Publikacja -
Fragmentomic analysis of biopeptides in silico released from milk proteins
PublikacjaThe fragmentomic-assisted method was employed to predict the biological potential of peptides derived from milk proteins hydrolyzed by papain and bromelain. Firstly, protein sequences were acquired from the BIOPEP-UWM database and then hydrolyzed by the above enzymes using a BIOPEP-UWM tool called “Enzyme(s) action”. The released peptides were defined as parent peptides and further analyzed for the presence of shorter peptidic...
-
Modeling the Structure, Dynamics, and Transformations of Proteins with the UNRES Force Field
PublikacjaThe physics-based united-residue (UNRES) model of proteins ( www.unres.pl ) has been designed to carry out large-scale simulations of protein folding. The force field has been derived and parameterized based on the principles of statistical-mechanics, which makes it independent of structural databases and applicable to treat nonstandard situations such as, proteins that contain D-amino-acid residues. Powered by Langevin dynamics...
-
Analysis of the Factors Affecting Static In Vitro Pepsinolysis of Food Proteins
PublikacjaIn this meta-analysis, we collected 58 publications spanning the last seven decades that reported static in vitro protein gastric digestion results. A number of descriptors of the pepsinolysis process were extracted, including protein type; pepsin activity and concentration; protein concentration; pH; additives; protein form (e.g., ‘native’, ‘emulsion’, ‘gel’, etc.); molecular weight of the protein; treatment; temperature; and...
-
Modeling SARS‐CoV‐2 proteins in the CASP‐commons experiment
PublikacjaCritical Assessment of Structure Prediction (CASP) is an organization aimed at advancing the state of the art in computing protein structure from sequence. In the spring of 2020, CASP launched a community project to compute the structures of the most structurally challenging proteins coded for in the SARS-CoV-2 genome. Forty-seven research groups submitted over 3000 three-dimensional models and 700 sets of accuracy estimates on...
-
Occlusion phenomenon of redox probe by protein as a way of voltammetric detection of non-electroactive C-reactive protein
Publikacja -
Application of Barkhausen method to determine internal stresses in cold-rolled and hot-rolled sheets
PublikacjaThe paper presents investigations on internal stress distribution in plane hot—or cold-rolled sheets dedicated for the cold-formed profiles. The motivation of the research is a new profile design whose bending technology, incorporating a longitudinal welded joint and hot-dip galvanization after bending and welding introduces additional internal stresses. In order to identify the entirety of stresses in the process of sheet production...
-
Fracture in Asymmetric Bonded Joints
PublikacjaAdhesion was studied in asymmetric bonded joints using fracture mechanics tests. The asymmetric bonded joints consist of two different type and/or thickness materials bonded by an adhesive. Mentions of asymmetric bonded joint tests employed so far are rare in the literature. They are imperfect and therefore are not standardized. Accordingly three new tests were introduced in this work to study bonded joints. The new metrological...
-
Thermoplastic Elastomers Filled With GTR
PublikacjaIn recent years, searching for new and cost-effective methods of recycling waste tires has been a subject of research in many laboratories worldwide. This is due to regulations in the law and environmental factors, which have both stimulated industry to re-use high quality rubber found in ground tire rubber (GTR). One of the most promising solutions in this area seems to be thermoplastic elastomers (TPEs) filled with GTR, which...
-
Iron–Sulfur Cluster Biogenesis Chaperones: Evidence for Emergence of Mutational Robustness of a Highly Specific Protein–Protein Interaction
PublikacjaBiogenesis of iron–sulfur clusters (FeS) is a highly conserved process involving Hsp70 and J-protein chaperones. However, Hsp70 specialization differs among species. In most eukaryotes, including Schizosaccharomyces pombe, FeS biogenesis involves interaction between the J-protein Jac1 and the multifunctional Hsp70 Ssc1. But, in Saccharomyces cerevisiae and closely related species, Jac1 interacts with the specialized Hsp70 Ssq1,...
-
Partial characterization of proteins from mussel Mytilus galloprovincialis as a biomarker of contamination
PublikacjaPrzedmiotem zainteresowania były:- funkcjonalne i przeciwutleniające właściwości białek małża Mytilus galloprovincialis,- poziom zawartości metali ciężkich (ołów i kadm) w tkankach.Badania przeprowadzono dla małży zebranych zarówno w zanieczyszczonych, jak i w czystych obszarach strefy brzegowej Morza Czarnego w regionie Warny (Bułgaria). W badaniach wykorzystano takie techniki analityczne jak: spektroskopia FTIR, fluorescencja,...
-
The Extracellular Bacterial HtrA Proteins as Potential Therapeutic Targets and Vaccine Candidates
Publikacja -
Evolution of physics-based methodology for exploring the conformational energy landscape of proteins
Publikacja -
Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria
PublikacjaSingle-stranded DNA-binding proteins (SSBs) are indispensable elements in the cells of all living organisms. SSB proteins interact with ssDNA insequence in an independent manner, preventing them from forming secondary structures and from degradation by nucleases. In this way, SSB-binding proteins participate in all processes involving ssDNA, such as replication, repair and recombination. Although there are differences in amino...
-
Coarse-grained simulation - an efficient approach for studying motions of large proteins
PublikacjaOne of the most important challenges in performing Molecular Dynamics (MD) simulations of large protein complexes is to accommodate the model accuracy and the simulation timescale. Hitherto, for the most relevant dynamics of protein aggregates in an explicit aqueous environment, the timescale reachable for the all-atoms simulations is of hundreds of nanoseconds. This range is four to six orders of magnitude smaller than processes...
-
Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria
PublikacjaSingle-stranded DNA-binding proteins (SSBs) are indispensable elements in the cells of all living organisms. SSB proteins interact with ssDNAinsequence in an independent manner, preventing them from forming secondary structures and from degradation by nucleases. In this way, SSB-binding proteins participate in all processes involving ssDNA, such as replication, repair and recombination.Although there are differences in amino acid...
-
Porcine myofibrillar proteins as potential precursors of bioactive peptides – an in silico study
Publikacja -
Analysis of differences in the stability of proteins encoded in mitochondrial DNA of model organisms
Publikacja -
Generalization of the Elastic Network Model for the Study of Large Conformational Changes in Proteins
Publikacja -
General Mechanism of Osmolytes’ Influence on Protein Stability Irrespective of the Type of Osmolyte Cosolvent
PublikacjaThe stability of proteins in an aqueous solution can be modified by the presence of osmolytes. The hydration sphere of stabilizing osmolytes is strikingly similar to the enhanced hydration sphere of a protein. This similarity leads to an increase in the protein stability. Moreover, the hydration sphere of destabilizing osmolytes is significantly different. These solutes generate in their surroundings so-called “structurally different...
-
The investigation of the effects of counterions in protein dynamics simulations
Publikacja -
Some applications of thermophiles and their enzymes for protein processing
PublikacjaPrzedstawiono charakterystykę, właściwości oraz możliwości zastosowań enzymów proteolitycznych wytwarzanych przez termofile. Zaletą tych biokatalizatorów jest między innymi duża odporność na działanie rozpuszczalników organicznych, detergentów i innych czynników denaturujących białka oraz mała wrażliwość na zmiany pH. Dokonano też przeglądu czynników powodujących dużą stabilność proteaz z termofili.
-
Modelling gene expression of a self-regulating protein
PublikacjaWe analyze a model of gene transcription and protein synthesis. We take into account the number of sites on the protein’s promoter at which the protein’s dimers can bind blocking transcription of protein mRNA.
-
Pea Protein for Hempseed Oil Nanoemulsion Stabilization
Publikacja -
Polysaccharide–Protein Complexes in a Coarse-Grained Model
Publikacja -
ANALYSIS OF THE p53 PROTEIN GENE EXPRESSION MODEL
PublikacjaWe study the asymptotic behaviour of the solutions of the p53-Mdm2 model proposed by Monk (2003). The p53 gene is crucial for cellular inhibition of the angiogenesis process, while Mdm2 is a negative regulator of the p53 tumor-suppressor. We investigate the stability of the positive steady state and perform some numerical experiments.
-
Protein thermal stabilization in aqueous solutions of osmolytes
PublikacjaProteins’ thermal stabilization is a significant problem in various biomedical, biotechnological, and technological applications. We investigated thermal stability of hen egg white lysozyme in aqueous solutions of the following stabilizing osmolytes: Glycine (GLY), N-methylglycine (NMG), N,N-dimethylglycine (DMG), N,N,N-trimethylglycine (TMG), and trimethyl-N-oxide (TMAO). Results of CD-UV spectroscopic investigation were compared...
-
Apple pectin complexes with whey protein isolate
Publikacja -
Hydrodynamics of tank partially filled with liquid.
PublikacjaW artykule podano podstawowe równania dynamiki zbiornika wypełnionego częściowo cieczą. Na modelu tego zbiornika przeprowadzono badania eksperymentalne i wyniki tych badań przedstawiono jako efekt końcowy.
-
Forming of explosively welded metal plates.
PublikacjaW pracy przedstawiono możliwości kształtowania plastycznego poprzez walcowanie, tłoczenie, gięcie, skręcanie blach wielowarstwowych zgrzewanych wybuchowo.
-
On the crack front curvature in bonded joints
PublikacjaStandard tests of adhesively bonded specimens are likely to produce heterogeneous stress distribution along the crack front and its vicinity. High separation rate mode I dominated fracture test is performed.Observation of post mortem fractured surfaces with an optical microscope reveals characteristic features of mixed mode I/III fracture near the sides of the specimen but not in the middle. At first, finite elements calculations...
-
Underwater welded joint properties investigation
PublikacjaPrzeprowadzono badania mikro- i makroskopowe złączy spawanych typu implant wykonanych pod wodą metodą lokalnej komory suchej. Wyniki badań świadczą o tym, że w SWC badanych złączy tworzą się kruche struktury hartownicze, które są odpowiedzialne za zwiększoną skłonność stali o podwyższonej wytrzymałości do tworzenia pęknieć zimnych. Analiza rozkładów twardości złączy implant również potwierdza istnienie szkodliwego wpływu środowiska...
-
Modelling of vibrations of a liquid filled tank
PublikacjaPrzeprowadzono analizę teoretyczną i numeryczną zbiorników długich i krótkich wypełnionych całkowicie lub częściowo cieczą. Wyznaczenie obciążeń hydrodynamicznych dotyczy bezpośrednio wytrzymałości konstrukcji samego zbiornika, a przez to pozwala określić wytrzymałość konstrukcyjną całego kadłuba. Szczególne wymagania stawiane są zbiornikom do przewozu cieczy radioaktywnych, gdyż należy zapewnić bardzo wysoki stopień bezpieczeństwa...
-
Stability of an imperfect truss loaded by wind
PublikacjaThe present paper is devoted to the numerical research of stability of a truss loaded by wind and stiffened by elastic supports located at the top chord. The lateral braces or lateral and torsional braces were taken into account. In this paper, the linear buckling analysis re- sults for the beam and shell model were presented. Two different shapes of initial geometric imperfections were considered in the non-linear static analysis...
-
Hazard prevention by forced time constraints
PublikacjaMinimalne Zbiory Przyczyn powstałe podczas analizy Drzew Błędów wskazują zdarzenia, które jeśli nastąpią razem, mogą doprowadzić do Hazardu. W niektórych zastosowaniach opis zdarzeń może zostać poszerzony o informację czasową, wtedy zdarzenia z MZP mogą doprowadzić do Hazardu tylko jeśli wystąpi pomiędzy nimi określona zależność czasowa. W tym artykule przedstawione zostało jak informacja czasowa może zostać wprowadzona do Drzew...
-
Single-stranded DNA-binding proteins (SSBs) - sources and applicationsin molecular biology
PublikacjaBiałka wiążące się do jednoniciowego DNA (SSB) odgrywają ważną rolę w replikacji, rekombinacji i naprawie DNA u bakterii, archea i eukariota. Ostatnio wzrosło zainteresowanie białkami SSB jako przydatnymi narzędziami w metodach badawczych biologii molekularnej, analitycznych i diagnostycznych. Większość znanych prokariotycznych białek SSB zbudowanych jest z czterech podjednostek (homotetramer) z jedną domeną wiążąca (OB) jednoniciowe...
-
Extension of the UNRES Coarse-Grained Force Field to Membrane Proteins in the Lipid Bilayer
Publikacja -
Why the solvation water around proteins is more dense than bulk water
PublikacjaThe main aim of this work is to propose a rational explanation of commonly observed phenomenon of increasing water density within solvation shell of proteins. We have observed that geometry of the water-water hydrogen bond network within solvation layer differs from the one in bulk water and it is the effect of interactions of water molecules with protein surface. Altered geometry of the network reflects changes in the structure...
-
Why the Solvation Water around Proteins Is More Dense than Bulk Water
PublikacjaThe main aim of this work is to propose a rational explanation of the commonly observed phenomenon of increasing water density within solvation shell of proteins. We have observed that the geometry of the water–water hydrogen bond network within solvation layer differs from the one in bulk water, and it is the result of interactions of water molecules with protein surface. Altered geometry of the network reflects changes in the...
-
Crystal structures of the Bacillus subtilis prophage lytic cassette proteins XepA and YomS
Publikacja