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A new B-D-galactosidase with a low temperature optimum isolated from the Antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization.
PublikacjaA psychrotrophic bacterium producing a coldadaptedB-galactosidase upon growth at low temperatureswas classiWed as Arthrobacter sp. 20B. A genomic DNAlibrary of strain 20B introduced into Escherichia coliTOP10F' and screening on X-Gal (5-bromo-4-chloro-3-indolyl-B-D-galactopyranoside)-containing agar plates ledto the isolation of B-galactosidase gene. The B-galactosidasegene (bgaS) encoding a protein of 1,053 amino acids,with a...
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Wykorzystanie różnych form opadów i osadów atmosferycznych do oceny zanieczyszczenia środowiska w różnych regionach geograficznych Polski - część II - rosa = Various forms of atmospheric precipitation and deposits as a measure of environmental pollution in different geographic regions of Poland - part II - dew
PublikacjaW ramach pracy przeprowadzono analizy 235 próbek rosy i 65 próbek deszczu zebranych na 8 stacjach na terenie Polski w okresie od sierpnia 2004 do listopada 2006 roku. W zależności od położenia oraz charakteru tła emisji zanieczyszczeń stacje pogrupowano na: rolnicze (Wrocław, Dziemiany); miejskie w strefie wybrzeża (Gdańsk, Gdynia, Sopot); miejskie, położone poza strefą wybrzeża (Bytów, Kraków, Mława) Próbki opadu i osadu atmosferycznego...
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Pharmaceuticals wastewater treatment via different advanced oxidation processes: Reaction mechanism, operational factors, toxicities, and cost evaluation – A review
PublikacjaPharmaceuticals comprises different drugs used for treating different infections in human being and animals. The huge quantities of pharmaceuticals used are found to discharge into different water resources and cause different health and environmental problems. The continuous and large-scale discharge of pharmaceuticals increases their persistency which can consequently make them more toxic. The different advanced oxidation processes...
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Proteases immobilized on nanomaterials for biocatalytic, environmental and biomedical applications: Advantages and drawbacks
PublikacjaProteases have gained significant scientific and industrial interest due to their unique biocatalytic characteristics and broad-spectrum applications in different industries. The development of robust nanobiocatalytic systems by attaching proteases onto various nanostructured materials as fascinating and novel nanocarriers has demonstrated exceptional biocatalytic performance, substantial stability, and ease of recyclability over...
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High-performance activation of ozone by sonocavitation for BTEX degradation in water
PublikacjaThis work presents a novel advanced oxidation process (AOP) for degradation of emerging organic pollutants – benzene, toluene, ethylbenzene and xylenes (BTEXs) in water. A comparative study was performed for sonocavitation assisted ozonation under 40–120 kHz and 80–200 kHz dual frequency ultrasounds (DFUS). Based on the obtained results, the combination of 40–120 kHz i.e., low-frequency US (LFDUS) with O3 exhibited excellent oxidation...
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Optimization of vortex-assisted supramolecular solvent-based liquid liquid microextraction for the determination of mercury in real water and food samples
PublikacjaA novel method was developed for sample preparation for spectrophotometric determination of Hg(II) in water and food samples. The method was based on vortex-assisted supramolecular solvent-assisted liquid-liquid microextraction (VA-SUPRASs-LLME). Analytical parameters such as pH, chelating agent, solvent type and volume, vortex time and salting out effect were optimized. Surface and normal probability plots were drawn for the variables...
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In vitro enzyme kinetics and NMR-based product elucidation for glutathione S-conjugation of the anticancer unsymmetrical bisacridine C-2028 in liver microsomes and cytosol: major role of glutathione S-transferase M1-1 isoenzyme
PublikacjaThis work is the next step in studying the interplay between C-2028 (anticancer-active unsymmetrical bisacridine developed in our group) and the glutathione S-transferase/glutathione (GST/GSH) system. Here, we analyzed the concentration- and pH-dependent GSH conjugation of C-2028 in rat liver microsomes and cytosol. We also applied three recombinant human GST isoenzymes, which altered expression was found in various tumors. The...
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Bovine Serum Albumin – Hydroxyapatite Nanoflowers as Potential Local Drug Delivery System of Ciprofloxacin
PublikacjaIntroduction: Hybrid nanoflowers are structures consisting of organic (enzymes, proteins, nucleic acids) and inorganic components (mostly metal phosphates) with a flower-like hierarchical structure. Novel hybrid nanoflowers based on bovine serum albumin (BSA) and hydroxyapatite (HA) were obtained and characterized. Study on BSA-HA nanoflowers as potential drug delivery system is reported for the first time. Methods: Embedding ciprofloxacin...
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Feasibility Study of Biohydrogen Production from Acid Cheese Whey via Lactate-Driven Dark Fermentation
PublikacjaThe high loading of lactic acid bacteria (LAB) present in cheese whey still limits its use as hydrogen feedstock. This study aims to investigate the feasibility of producing hydrogen from acid cheese whey via lactate-driven dark fermentation (LD-DF). Mesophilic batch fermentations were performed with delipidated acid cheese whey at a fixed pH of 5.8 and driven by an acidogenic bacterial culture containing LAB and lactate-oxidizing...
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Cu-HKUST-1 and Hydroxyapatite–The Interface of Two Worlds toward the Design of Functional Materials Dedicated to Bone Tissue Regeneration
PublikacjaA novel composite based on biocompatible hydroxyapatite (HA) nanoparticles and Cu-HKUST-1 (Cu-HKUST-1@HA) has been prepared following a layer-by-layer strategy. Cu-HKUST-1 was carefully selected from a group of four Cu-based metal−organic frameworks as the material with the most promising antimicrobial activity. The formation of a colloidal Cu- HKUST-1 layer on HA nanoparticles was confirmed by various techniques, e.g., infrared...
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New pathways for functionalization of phosphorus ligands
PublikacjaThe presented dissertation consistsof two main partsand focuses on the synthesis and characterization of new low-valent phosphorus compounds. Thesestudies have already been fully described in three JCR-journals. The first partdescribes the utilization ofselectedphosphanylphosphinidene transition metal complexes as a source of diversified phosphorus ligands. For thispurposethree complexes...
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A High-Arctic flow-through lake system hydrochemical changes: Revvatnet, southwestern Svalbard (years 2010–2018)
PublikacjaLake ecosystems are strongly coupled to features of their surrounding landscapes such as geomorphology, lithology, vegetation and hydrological characteristics. In the 2010–2018 summer seasons, we investigated an Arctic flow-through lake system Revvatnet, located in the vicinity of the coastal zone of Hornsund fjord in Svalbard, characterising its hydrological properties and the chemical composition of its waters. The lake system...
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N-doped carbon nanospheres as selective fluorescent probes for mercury detection in contaminated aqueous media: chemistry, fluorescence probing, cell line patterning, and liver tissue interaction
PublikacjaA precise nano-scale biosensor was developed here to detect Hg2+ in aqueous media. Nitrogen-doped carbon nanospheres (NCS) created from the pyrolysis of melamine–formaldehyde resin were characterized by FESEM, XRD, Raman spectra, EDS, PL, UV–vis spectra, and N2 adsorption–desorption, and were used as a highly selective and sensitive probe for detecting Hg2+ in aqueous media. The sensitivity of NCS to Hg2+ was evaluated by photoluminescence...
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Influence of Phosphorus Speciation on Its Chemical Removal from Reject Water from Dewatering of Municipal Sewage Sludge
PublikacjaThe aim of the presented research was the assessment of phosphorus speciation impact on the precipitation of phosphorus in reject water using Ca(OH)2. To achieve this, phosphorus speciation (organic and inorganic phosphorus in suspension and in dissolved form) in reject water that is produced during sludge dewatering, after methane digestion in wastewater treatment plants (WWTPs), was determined. This study covered the materials...
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Artificial neural network prophecy of ion exchange process for Cu (II) eradication from acid mine drainage
PublikacjaThe removal of heavy metal ions from wastewater was found to be significant when the cation exchange procedure was used effectively. The model of the cation exchange process was built using an artificial neural network (ANN). The acid mine drainage waste’s Cu(II) ion was removed using Indion 730 cation exchange resin. Experimental data from 252 cycles were recorded. In a column study, 252 experimental observations validated the...
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Optimization of carbamazepine photodegradation on defective TiO2-based magnetic photocatalyst
PublikacjaIn this work, carbamazepine (CBZ) degradation over defective Fe3O4@SiO2/d-TiO2/Pt photocatalyst was studied. Within the titania structure, Ti vacancies and Pt nanoparticles were introduced to enhance the photocatalyst’s light absorption and influence charge carriers’ mobility. For the carbamazepine degradation, process parameters, e.g., temperature, flux intensity, photocatalyst loading, aeration, pH, and addition of H2O2, were optimized...
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Molecular mechanism of proton-coupled ligand translocation by the bacterial efflux pump EmrE
PublikacjaThe current surge in bacterial multi-drug resistance (MDR) is one of the largest challenges to public health, threatening to render ineffective many therapies we rely on for treatment of serious infections. Understanding different factors that contribute to MDR is hence crucial from the global “one health” perspective. In this contribution, we focus on the prototypical broad-selectivity proton-coupled antiporter EmrE, one of the...
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Spatial Differences in the Chemical Composition of Surface Water in the Hornsund Fjord Area: A Statistical Analysis with A Focus on Local Pollution Sources
PublikacjaSurface catchments in Svalbard are sensitive to external pollution, and yet what is frequently considered external contamination may originate from local sources and natural processes. In this work, we analyze the chemical composition of surface waters in the catchments surrounding the Polish Polar Station in Svalbard, Hornsund fjord area. We have pooled unpublished and already published data describing surface water composition...
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Treatment Wetland effluent quality improvement by usage sorbents of various origin
PublikacjaTWs are finding as low-cost and also effective system to treat domestic wastewater. The aim of this study is to find suitable adsorbing material for application in an additional treatment unit where treatment wetlands do not provide sufficient PO4 3- reduction level. Material M1 is a fine-grained by-product of thermal treatment of carbonate-siliceous rock (opoka) with high content of calcium carbonate CaCO3 in temperature 700°C....
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Application of micellar electrokinetic chromatography for detection of silver nanoparticles released from wound dressing
PublikacjaThe recent emergence of nanotechnology has provided a new therapeutic modality in case of silver nanoparticles. Dressings containing silver form the basis for the treatment of burns and wounds, either acute or chronic ones. The aim of the study was to examine silver release from the different wound dressings: commercially available (Atrauman Ag, Aqua- cel Ag) and experimental (FKDP-AgNPs) using MEKC. In order to characterize prepared keratin...
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Assessment of the Bulgarian Wastewater Treatment Plants’ Impact on the Receiving Water Bodies
PublikacjaDeterioration of water quality is a major problem world widely according to many international non-governmental organizations (NGO). As one of the European Union (EU) countries, Bulgaria is also obliged by EU legislation to maintain best practices in assessing surface water quality and the efficiency of wastewater treatment processes. For these reasons studies were undertaken to utilize ecotoxicological (Microtox®, Phytotoxkit...
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Cu( II ) ions removal from wastewater using starch nanoparticles ( SNPs ): An Eco‐sustainable approach
PublikacjaThe complex structured starch particles were reduced to the nanoscale size range through hydrolysis utilizing low concentration acid assisted with ultrasound irradiation. The synthesized starch nanoparticles (SNPs) were characterized by TEM, FTIR, and XRD techniques. The synthesized SNPs possessed surface activated entities, as many cationic functional groups were confirmed through the FTIR spectrum. Also, these SNPs were effectively...
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Functionalized nanodiamonds as a perspective green carbo-catalyst for removal of emerging organic pollutants
PublikacjaRapid industrial and urban development jointly with rising global population strongly affect the large-scale issues with drinking, groundwater, and surface water pollution. Concerns are not limited to environmental issues but also human health impact becoming serious global aspect. Organic pollution becomes a primarily serious hazard, therefore, the novel sophisticated approaches to treat them are thoroughly investigated. Among...
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A New Expression System Based on Psychrotolerant Debaryomyces macquariensis Yeast and Its Application to the Production of Cold-Active β-D-Galactosidase from Paracoccus sp. 32d
PublikacjaYeasts provide attractive host/vector systems for heterologous gene expression. The currently used yeast-based expression platforms include mesophilic and thermotolerant species. A eukaryotic expression system working at low temperatures could be particularly useful for the production of thermolabile proteins and proteins that tend to form insoluble aggregates. For this purpose, an expression system based on an Antarctic psychrotolerant...
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Potential degradation of norfloxacin using UV-C/Fe2+/peroxides-based oxidative pathways
PublikacjaThe removal of norfloxacin (NOR), a widely used pharmaceutical and emerging water pollutant, was studied using UV-C and Fe2+ catalyzed peroxides-based oxidative processes (e.g., UV-C/Fe2+/H2O2, UV-C/Fe2+/S2O82− and UV-C/Fe2+/HSO5−) and compared with UV-C and UV-C/Fe2+. The UV-C and UV-C/Fe2+ degraded NOR to 38 and 55 %. However, use of peroxides, i.e., H2O2, S2O82−, HSO5− with UV-C and UV-C/Fe2+ promoted NOR %degradation to 75,...
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A method to monitor urinary carbon dioxide in patients with septic shock
PublikacjaSevere sepsis and septic shock are life-threatening conditions with mortality rates exceeding 31% (Levy et al., 2012) [1]. Septicemia was the most expensive US hospital condition in 2011 (Torio and Andrews, 2006)[2]. Urinary carbon dioxide may provide rapid, clinically useful information about a patient’s status, empowering physicians to intervene earlier and improve septic shock mortality. The objective of this paper is to validate...
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Wieloobszarowa rozmyta regulacja PID mocy reaktora jądrowego
PublikacjaW artykule przedstawiono wieloobszarowy regulator rozmyty z lokalnymi regulatorami PID dla sterowania mocą reaktora jądrowego typu PWR. Wykorzystano model matematyczny o parametrach skupionych reaktora PWR obejmujący procesy generacji i wymiany ciepła oraz efektów reaktywnościowych. Nastawy lokalnych regulatorów PID zostały dobrane w sposób optymalny, minimalizując całkowy wskaźnik jakości ISE. Na przykładzie pokazano że zastosowane...
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Refinement of the Hardening Soil model within the small strain range
PublikacjaThe popularity of the elasto-plastic Hardening Soil (HS) model is based on simple parameter identification from standard testing and empirical formulas. The HS model is implemented in many commercial FE codes designed to analyse geotechnical problems. In its basic version, the stress–strain behaviour within the elastic range is subject to the hypoelastic power law, which assures the barotropy of the elastic stiffness. However,...
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The magnetic field measurements in selected places of the industrial building
Dane BadawczeThe dataset is part of a comprehensive study on the assessment of the electromagnetic field intensity in a building of an operating industrial plant. Detailed results of magnetic field measurements, carried out in selected places of this building, are presented.
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The conducted immunity test of a power supply unit in accordance with EMC standards
Dane BadawczeThe dataset presents a result of measurements that are a part of immunity tests to conducted disturbances, induced by radio-frequency fields. The immunity tests were carried out on the mains cable of the DF1723003TC NDN power supply. Tests of immunity of electronic systems to conducted disturbances in the frequency range from 150 kHz to 230 MHz are...
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Principles and Developments of Solid-Phase Microextraction
PublikacjaSample preparation has been commonly considered a critical step of the analytical process. In this sense, remarkable efforts have been made to develop efficient sample preparation techniques which could overcome the limitations of conventional approaches. Since its inception in the early 1990’s, solid-phase microextraction (SPME) has become a widespread miniaturized sample preparation technique for extraction and preconcentration...
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Novel stationary phases based on asphaltenes for gas chromatography
PublikacjaWe present the results of investigations on the possibility of the application of the asphaltene fraction isolated from the oxidized residue from vacuum distillation of crude oil as a stationary phase for gas chromatography. The results of the investigation revealed that the asphaltene stationary phases can find use for the separation of a wide range of volatile organic compounds. The experimental values of Rohrschneider/McReynolds...
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THE APPLICATION OF KALMAN FILTERING TO PREDICT VERTICAL RAIL AXIS DISPLACEMENTS OF THE OVERHEAD CRANE BEING A COMPONENT OF SEAPORT TRANSPORT STRUCTURE
PublikacjaAs the devices designed to transport materials, the overhead cranes should meet certain geometric requirements for their operation to be safe. The presently available geodetic equipment, in particular total stations, provides opportunities for precise 3D measurements of coordinates of the controlled points. These coordinates make a basis for correcting the height of crane runway axes. The paper presents a method to calculate position...
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Damage detection in a bolted lap joint using guided waves
PublikacjaThe paper presents the experimental investigation of guided waves application to the condition assessment of prestressed bolted connections and a concept of new quantitative indicator. The main goal of the research was the analysis of the influence of the contact conditions changes to the characteristics of a propagating disturbance. The experimental tests were carried out for a single bolted lap joint. The excitation and acquisition...
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Selection of physiological parameters for optoelectronic system supporting behavioral therapy of autistic children
PublikacjaIn this article the procedure of selection of physiological parameters for optoelectronic system supporting behavioral therapy of autistic children is proposed. Authors designed and conducted an experiment in which a group of 30 health volunteers (16 females and 14 males) were examined. Under controlled conditions people were exposed to a stressful situation caused by the picture or sound (1kHz constant sound, which was gradually...
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System supporting behavioral therapy for children with autism
PublikacjaIn this paper, a system supporting behavioral therapy for autistic children is presented. The system consists of sensors network, base station and a brooch indicating person's emotional states. The system can be used to measure values of physiological parameters that are associated with changes in the emotional state. In the future, it can be useful to inform the autistic child and the therapist about the emotional state of the...
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Vegetable derived-oil facilitating carbon black migration from waste tire rubbers and its reinforcement effect
PublikacjaThree dimensional chemically cross-linked polymer networks present a great challenge for recycling and reutilization of waste tire rubber. In this work, the covalently cross-linked networks of ground tire rubber (GTR) were degraded heterogeneously under 150 °C due to the synergistic effects of the soybean oil and controlled oxidation. The degradation mechanism was discussed using Horikx theory and Fourier transformation infrared...
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Nano-structured Pt embedded in acidic salts of heteropolymolybdate matrices: MS EXAFS study
PublikacjaA structural study of Pt nano-catalysts is presented in the paper. The innovation in the case of the considered catalytic materials resides in the use a meso-microporous inorganic matrix of heteropolyacid salt of composition X2.5H0.5YMo12O40 where X = Rb, Cs and Y = P, Si as a catalyst support. Metallic nanoparticles were created by platinum ions reduction in a hot H2/Argon stream and were mainly located into matrix pores. As the...
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Emotional distress, burnout and sense of safety during the COVID-19 pandemic in teachers after the reopening of schools
PublikacjaThe COVID-19 pandemic is having a significant impact on people's psychological well-being and mental health. This study aimed to identify factors linked to emotional distress, burnout and sense of safety in teachers related to the reopening of Polish schools after lockdown, remote work, and the holiday period between March and August 2020. A total of 1,286 teachers from different educational institutions participated in the...
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Estimation of zero buoyancy of the batyscaphe. Pneumatic buoyancy control of the bathyscaphe
PublikacjaThe subjects of this work are matters concerning the batyscaphe designed and constructed by the Scientific Circle of Young Constructors (SCoYC) acting at the Faculty of Technical Sciences of the Olsztyn University of Warmia and Mazury.The goal od the remote-controlled batyscaphe is an underwater exploration of water resorvoirs in Poland. Design and construction of the unmanned watercraft, also known as a batyscaphe, required the...
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An in situ technique for the assessment of adhesive properties of a joint under load
PublikacjaSlow crack propagation in adhesive bonded joints has been characterised using an asymmetric wedge test. Crack position was evaluated from strain gauge measurements, both in the debonded partof the joint and in the bonded zone. Test temperature was changed during loading, giving insight into bond evolution. The technique allows accurate, and virtually continuous, determination of crack position to be made, and therefore the evaluation...
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Deterministic rendezvous of asynchronous bounded-memory agents in polygonal terrains
PublikacjaTwo mobile agents, modeled as points starting at differentlocations of an unknown terrain, have to meet. The terrain is a polygon with polygonal holes. We consider two versions of this rendezvous problem: exact RV, when the points representing the agents have to coincide at some time, and epsilon-RV, when these points have to get at distance less than epsilon in the terrain. In any terrain, each agent chooses its trajectory, but...
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Evolving gene regulatory networks controlling foraging strategies of prey and predators in an artificial ecosystem
PublikacjaCo-evolution of predators and prey is an example of an evolutionary arms race, leading in nature to selective pressures in positive feedback. We introduce here an artificial life ecosystem in which such positive feedback can emerge. This ecosystem consists of a 2-dimensional liquid environment and animats controlled by evolving artificial gene regulatory networks encoded in linear genomes. The genes in the genome encode chemical...
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The influence of the size of a one-faced metallic head in Janus nanoparticles as a co-catalyst on the photocatalytic efficiency of hydrogen evolution under vis light irradiation
PublikacjaJanus nanoparticles (NPs) consisting of MoOxSy nanospheres and silver (Ag) head, successfully developed by a simple, controlled method were in the first time they were applied as a co-catalysts in photocatalytic hydrogen generation reaction under vis light irradiation (λ > 420 nm). The MoOxSy-Ag as a co-catalysts were deposited on the obtained ZnIn2S4 microspheres (ZIS) using physical absorption method. The influence of the size...
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The influence of IT-competency dimensions on job satisfaction, knowledge sharing and performance across industries
PublikacjaPurpose – Technology makes knowledge management easier. Knowledge sharing is essential for organizational development. Job satisfaction fosters knowledge sharing. Hence, this study aims to develop an understanding of the mutual relationship between knowledge sharing and job satisfaction when both are predicted by information technology (IT)-competency dimensions such as IT-operations, IT-knowledge and IT-infrastructure in the context...
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Limited selectivity of amperometric gas sensors operating in multicomponent gas mixtures and methods of selectivity improvement
PublikacjaIn recent years, smog and poor air quality have became a growing environmental problem. There is a need to continuously monitor the quality of the air. The lack of selectivity is one of the most important problems limiting the use of gas sensors for this purpose. In this study, the selectivity of six amperometric gas sensors is investigated. First, the sensors were calibrated in order to find a correlation between the concentration...
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Modelling and Analysis of the Positioning Accuracy in the Loading Systems of Mobile Cranes
PublikacjaIn this work, the authors analyse the influence of the order and range of sequential movements of a crane's working members on the accuracy of the final cargo positioning. The analysis was conducted on the basis of a specially developed method in which the authors proposed the introduction of a geometrical indicator of positioning the load in the intermediate positions (after completing each movement sequence) and in the target...
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EXPERIMENTAL RESEARCH ON INFLUENCE OF THE FUEL NOZZLE GEOMETRY ON THE FUEL CONSUMPTION OF THE MARINE 4-STROKE DIESEL ENGINE
PublikacjaThe article presents experimental research that has been carried out on a marine, 4-stroke, 3-cylinder, turbocharged engine. During testing, the engine operated at a constant rotational speed of 750 rpm and a load from 0 kW to 280 kW. The engine was fuelled by diesel oil of known specification and loaded by electric generator with water resistance. The fuel consumption was measured during the engine operation with fuel nozzles...
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Evaluation of the Commercial Electrochemical Gas Sensors for the Monitoring of CO in Ambient Air
PublikacjaAir pollution is a growing concern of civilized world, which has a significant impact on human health and the environment. Recent studies highlight that the exposure to polluted air can increase the incidence of diseases and deteriorate the quality of life. Hence, it is necessary to develop tools for real-time air quality monitoring. For air pollution monitoring a wide range of stationary gas and particulate analysers can be used....
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Biopolymers and Their Composites for Drug Delivery: A Brief Review
PublikacjaIn recent years, excipient development has become a core area of research in pharmaceutical drug delivery because it influences the formulation development and drug delivery process in various ways. Polymeric drug delivery systems have been of great interest for controlled delivery as they show the great advantage in drug delivery systems because of optimized drug loading and releasing property. Then, the side effects of synthetic...