Wyniki wyszukiwania dla: CYP51A SEQUENCE
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3D printed ABS thermoplastic vs. steel. Dry sliding wear test in constant load & velocity ring on flat configuration. Test parameters: print layer thickness and orientation. Test symbol: 039_v_3
Dane BadawczeData gathered in sliding ring-on-block (flat contact) tribological experiment. Materials: alloy steel (heat treated) vs. ABS plastic.
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3D printed ABS thermoplastic vs. steel. Dry sliding wear test in constant load & velocity ring on flat configuration. Test parameters: print layer thickness and orientation. Test symbol: 019_v_3
Dane BadawczeData gathered in sliding ring-on-block (flat contact) tribological experiment. Materials: alloy steel (heat treated) vs. ABS plastic.
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3D printed ABS thermoplastic vs. steel. Dry sliding wear test in constant load & velocity ring on flat configuration. Test parameters: print layer thickness and orientation. Test symbol: 019_h_3
Dane BadawczeData gathered in sliding ring-on-block (flat contact) tribological experiment. Materials: alloy steel (heat treated) vs. ABS plastic.
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3D printed ABS thermoplastic vs. steel. Dry sliding wear test in constant load & velocity ring on flat configuration. Test parameters: print layer thickness and orientation. Test symbol: 039_h_5
Dane BadawczeData gathered in sliding ring-on-block (flat contact) tribological experiment. Materials: alloy steel (heat treated) vs. ABS plastic.
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3D printed ABS thermoplastic vs. steel. Dry sliding wear test in constant load & velocity ring on flat configuration. Test parameters: print layer thickness and orientation. Test symbol: 019_h_4
Dane BadawczeData gathered in sliding ring-on-block (flat contact) tribological experiment. Materials: alloy steel (heat treated) vs. ABS plastic.
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3D printed ABS thermoplastic vs. steel. Dry sliding wear test in constant load & velocity ring on flat configuration. Test parameters: print layer thickness and orientation. Test symbol: 039_v_2
Dane BadawczeData gathered in sliding ring-on-block (flat contact) tribological experiment. Materials: alloy steel (heat treated) vs. ABS plastic.
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3D printed ABS thermoplastic vs. steel. Dry sliding wear test in constant load & velocity ring on flat configuration. Test parameters: print layer thickness and orientation. Test symbol: 039_h_3
Dane BadawczeData gathered in sliding ring-on-block (flat contact) tribological experiment. Materials: alloy steel (heat treated) vs. ABS plastic.
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Differentiating patients with obstructive sleep apnea from healthy controls based on heart rate-blood pressure coupling quantified by entropy-based indices
PublikacjaWe introduce an entropy-based classification method for pairs of sequences (ECPS) for quantifying mutual dependencies in heart rate and beat-to-beat blood pressure recordings. The purpose of the method is to build a classifier for data in which each item consists of two intertwined data series taken for each subject. The method is based on ordinal patterns and uses entropy-like indices. Machine learning is used to select a subset...
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Augmenting digital documents with negotiation capability
PublikacjaActive digital documents are not only capable of performing various operations using their internal functionality and external services, accessible in the environment in which they operate, but can also migrate on their own over a network of mobile devices that provide dynamically changing execution contexts. They may imply conflicts between preferences of the active document and the device the former wishes to execute on. In the...
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Quasi-resonant DC-link voltage inverter with enhanced zero-voltage switching control
PublikacjaA new topology modification of the parallel quasi-resonant circuit for a dc-link voltage inverter enables regulation of the zero voltage dc-link subperiods and the dc-link voltage gradient settings. The proposed circuit is based on four MOSFET switches with free-wheeling diodes for controlled quasi-resonant recharging between L-C tank in order to assure inverter zero voltage switching (ZVS) conditions. Design optimization of the...
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Convex set of quantum states with positive partial transpose analysed by hit and run algorithm
PublikacjaThe convex set of quantum states of a composite K×K system with positive partial transpose is analysed. A version of the hit and run algorithm is used to generate a sequence of random points covering this set uniformly and an estimation for the convergence speed of the algorithm is derived. For K >3 or K=3 this algorithm works faster than sampling over the entire set of states and verifying whether the partial transpose is positive....
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Fusion of DNA-binding domain of Pyrococcus furiosus ligase with TaqStoffel DNA polymerase as a useful tool in PCR with difficult targets
PublikacjaThe DNA coding sequence of TaqStoffel polymer- ase was fused with the DNA-binding domain of Pyrococcus furiosus ligase. The resulting novel recombinant gene was cloned and expressed in E. coli. The recombinant enzyme was purified and its enzymatic features were studied. The fusion protein (PfuDBDlig-TaqS) was found to have enhanced processivity as a result of the conversion of the Taq DNA polymerase from a relatively low processive...
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A Power-Efficient Digital Technique for Gain and Offset Correction in Slope ADCs
PublikacjaIn this brief, a power-efficient digital technique for gain and offset correction in slope analog-to-digital converters (ADCs) has been proposed. The technique is especially useful for imaging arrays with massively parallel image acquisition where simultaneous compensation of dark signal non-uniformity (DSNU) as well as photo-response non-uniformity (PRNU) is critical. The presented approach is based on stopping the ADC clock by...
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The biological role of prolyl oligopeptidase and the procognitive potential of its peptidic inhibitors from food proteins
PublikacjaProlyl oligopeptidase (POP) is a conserved serine protease belonging to proline-specific peptidases. It has both enzymatic and non-enzymatic activity and is involved in numerous biological processes in the human body, playing a role in e.g., cellular growth and differentiation, inflammation, as well as the development of some neurodegenerative and neuropsychiatric disorders. This article describes the physiological and pathological...
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A Subspace-Splitting Moment-Matching Model-Order Reduction Technique for Fast Wideband FEM Simulations of Microwave Structures
PublikacjaThis article describes a novel model-order reduction (MOR) approach for efficient wide frequency band finite-element method (FEM) simulations of microwave components. It relies on the splitting of the system transfer function into two components: a singular one that accounts for the in-band system poles and a regular part that has no in-band poles. In order to perform this splitting during the reduction process, the projection...
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Measurement of Impulse Response of Shallow Water Communication Channel by Correlation Method
PublikacjaPerformances of underwater acoustic communication (UAC) systems are strongly related to specific propagation conditions of the underwater channel; conditions that can additionally change in time due to the movement of the acoustic system transmitter and receiver or to reflection by underwater objects of the transmitted signal. The time-varying impulse response is a comprehensive description of dynamically changing transmission...
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Visualization of a lifeboat motion during lowering along ship’s side
PublikacjaThis paper presents description of a computer program for motion visualization of a lifeboat lowered along ship’s side. The program is a post-processor which reads results of numerical calculations of simulated objects’ motions. The data is used to create scene composed of 3D surfaces to visualize mutual spatial positions of a lifeboat, ship’s side and water waving surface. Since the numerical data contain description of a simulation...
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CaZnO-based nanoghosts for the detection of ssDNA, pCRISPR and recombinant SARS-CoV-2 spike antigen and targeted delivery of doxorubicin
PublikacjaOverexpression of proteins/antigens and other gene-related sequences in the bodies could lead to significant mutations and refractory diseases. Detection and identification of assorted trace concentrations of such proteins/antigens and/or gene-related sequences remain challenging, affecting different pathogens and making viruses stronger. Correspondingly, coronavirus (SARS-CoV-2) mutations/alterations and spread could lead to overexpression...
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Method-Dependent Epidemiological Cutoff Values for Detection of Triazole Resistance in Candida and Aspergillus Species for the Sensititre YeastOne Colorimetric Broth and Etest Agar Diffusion Methods
PublikacjaAlthough the Sensititre Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candida or Aspergillus species. We collected fluconazole, itraconazole, posaconazole, and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method/agent-dependent) 11,171 Candida albicans, 215 C. dubliniensis, 4,418 C. glabrata species complex,...
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Limiting distribution of Lempel Ziv'78 redundancy
PublikacjaWe show that the Lempel Ziv'78 redundancy rate tends to a Gaussian distribution for memoryless sources. We accomplish it by extending findings from our 1995 paper [3]. We present a new simplified proof of the Central Limit Theorem for the number of phrases in the LZ'78 algorithm. As in our 1995 paper, here we first analyze the asymptotic behavior of the total path length in a digital search tree (a DST) built from independent sequences....
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On the Limiting distribution of Lempel Ziv'78 Redundancy for Memoryles Sources
PublikacjaWe show that the Lempel Ziv'78 redundancy rate tends to a Gaussian distribution for memoryless sources. We accomplish it by extending findings from our 1995 paper [3]. We present a new simplified proof of the Central Limit Theorem for the number of phrases in the LZ'78 algorithm. As in our 1995 paper, here we first analyze the asymptotic behavior of the total path length in a digital search tree (a DST) built from independent sequences....
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Quaternion encryption methods for multimedia transmission, a survey of existing approaches
PublikacjaIn this paper we review quaternion encryption methods for multimedia transmission. We explain their weak and strong properties as well as suggest possible modifications. Our main focus is an algorithm QFC presented in paper by Dzwonkowski et al. (2015). All encryption methods, presented in this paper, use special properties of quaternions to perform rotations of data sequences in 3D space. Each method uses a common key generation...
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Naturally occurring mitochondrial DNA heteroplasmy in the MRL mouse
PublikacjaThe MRL/MpJ mouse is an inbred laboratory strain of Mus musculus, known to exhibit enhanced autoimmunity, increased wound healing, and increased regeneration properties. We report the full-length mitochondrial DNA (mtDNA) sequence of the MRL mouse (Accession # EU450583), and characterize the discovery of two naturally occurring heteroplasmic sites. The first is a T3900C substitution in the TpsiCloop of the tRNA methionine gene...
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In silico epitope prediction of Borrelia burgdorferi sensu lato antigens for the detection of specific antibodies
PublikacjaDespite many years of research, serodiagnosis of Lyme disease still faces many obstacles. Difficulties arise mainly due to the low degree of amino acid sequence conservation of the most immunogenic antigens among B. burgdorferi s.l. genospecies, as well as differences in protein production depending on the environment in which the spirochete is located. Mapping B-cell epitopes located on antigens allows for a better understanding...
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Wild oscillations in a nonlinear neuron model with resets: (I) Bursting, spike-adding and chaos
PublikacjaIn a series of two papers, we investigate the mechanisms by which complex oscillations are generated in a class of nonlinear dynamical systems with resets modeling the voltage and adaptation of neurons. This first paper presents mathematical analysis showing that the system can support bursts of any period as a function of model parameters, and that these are organized in a period-incrementing structure. In continuous dynamical...
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Modeling SARS‐CoV‐2 proteins in the CASP‐commons experiment
PublikacjaCritical Assessment of Structure Prediction (CASP) is an organization aimed at advancing the state of the art in computing protein structure from sequence. In the spring of 2020, CASP launched a community project to compute the structures of the most structurally challenging proteins coded for in the SARS-CoV-2 genome. Forty-seven research groups submitted over 3000 three-dimensional models and 700 sets of accuracy estimates on...
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Open smart glasses development platform for AAL applications
PublikacjaThis paper describes an open platform for multi sensory electronic glasses that supports new and enhanced methods for intelligent interaction with patients, with smart objects, or to be used as new data input modalities like proximity sensor or smart textile interfaces. All the activities have been developed, investigated and evaluated within EU CHIST-ERA eGlasses project...
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Passing from requirements specification to class model using application domain ontology
PublikacjaThe quality of a classic software engineering process depends on the completeness of project documents and on the inter-phase consistency. In this paper, a method for passing from the requirement specification to the class model is proposed. First, a developer browses the text of the requirements, extracts the word sequences, and places them as terms into the glossary. Next, the internal ontology logic for the glossary needs to...
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Uniwersalny program komputerowy do projektowania zmiany kierunku trasy
PublikacjaW pracy przedstawiono kolejny program komputerowy stanowiący implementację algorytmu obliczeniowego dotyczącego projektowania zmiany kierunku trasy kolejowej, dostosowanego do techniki ciągłych pomiarów satelitarnych. Program ten ma charakter uniwersalny, gdyż w ogólnym przypadku obejmuje wprowadzenie do układu geometrycznego dwóch łuków kołowych o różnym promieniu, czyli zastosowanie łuku koszowego. Może się to okazać szczególnie...
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Measurements of the mechanical system properties of "SpeedLine magnetic" ultra-fast robot prototype for IML labeling
Dane BadawczeThe mechanical system tests of the high-speed IML labeling robot "SpeedLine Magnetic" were carried out in order to analyze the effects of the drive system's operating parameters on vibration and noise.
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Chicken feather keratin as a source of bioactive peptides useful in prevention of metabolic disorders – in silico and in vitro hydrolysis
PublikacjaProtein derived bioactive peptides not only serve as nutrients but can also exert drug-like activity, e.g. antidiabetic, antihypertensive, or antimicrobial, to name a few. Most biopeptides have beneficial effects on health that make them attractive for nutraceutical applications. The majority of bioactive peptides have been obtained from food proteins, but protein-rich by-products generated by agriculture...
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The Imidazoacridinone Antitumor Drug, C-1311, Is Metabolized by Flavin Monooxygenases but Not by Cytochrome P450s.
PublikacjaPrezentowane w publikacji wyniki badań mają na celu wyjaśnienie roli enzymów wątrobowych w metabolizmie przeciwnowotworowych pochodnych C-1311 oraz C-1330. Oba związki inkubowane były z frakcją mikrosomalną oraz enzymami rekombinantowymi należącymi do grypy cytochromu P450, FMO oraz UGT. Badania wykazały, że oba związki są metabolizowane przez enzymy frakcji mikrosomalnej, ale nie przez enzymy cytochromu P450. Aktywność enzymatyczna...
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Interactions with recognized objects
PublikacjaImplicit interaction combined with object recognition techniques opens a new possibility for gathering data and analyzing user behavior for activity and context recognition. The electronic eyewear platform, eGlasses, is being developed, as an integrated and autonomous system to provide interactions with smart environment. In this paper we present a method for the interactions with the recognized objects that can be used for electronic...
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Remote Estimation of Video-Based Vital Signs in Emotion Invocation Studies
PublikacjaAbstract— The goal of this study is to examine the influence of various imitated and video invoked emotions on the vital signs (respiratory and pulse rates). We also perform an analysis of the possibility to extract signals from sequences acquired with cost-effective cameras. The preliminary results show that the respiratory rate allows for better separation of some emotions than the pulse rate, yet this relation highly depends...
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Comparative Study of Machining Technology Selection to Manufacture Large-Size Components of Offshore Constructions
PublikacjaThe focus of this paper is on process planning for large parts manufacture in systems of definite process capabilities, involving the use of multi-axis machining centres. The analysis of machining heavy mechanical components used in off-shore constructions has been carried out. Setup concepts applied and operation sequences determined in related process plans underwent studies. The paper presents in particular a reasoning approach...
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Adaptive Sampling for Non-intrusive Reduced Order Models Using Multi-Task Variance
PublikacjaNon-intrusive reduced order modeling methods (ROMs) have become increasingly popular for science and engineering applications such as predicting the field-based solutions for aerodynamic flows. A large sample size is, however, required to train the models for global accuracy. In this paper, a novel adaptive sampling strategy is introduced for these models that uses field-based uncertainty as a sampling metric. The strategy uses...
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Storing Matter’technique in SIMS depth profile analysis
PublikacjaSecondary ion mass spectrometry (SIMS) is a very useful technique for the analysis of layered systems. It is based on the primary ion beam sputtering of solids and mass analysis of the emitted secondary ions. A main limitation of this technique results from the direct quantitative analysis, since the ionization efficiency of a given atom is highly influenced by the neighbouring atoms at the surface. This phenomenon is known as...
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Modelling and Analysis of the Positioning Accuracy in the Loading Systems of Mobile Cranes
PublikacjaIn this work, the authors analyse the influence of the order and range of sequential movements of a crane's working members on the accuracy of the final cargo positioning. The analysis was conducted on the basis of a specially developed method in which the authors proposed the introduction of a geometrical indicator of positioning the load in the intermediate positions (after completing each movement sequence) and in the target...
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A new cold-active β-galactosidase from Arthrobacter sp. S3* - gene cloning, overexpression, purification and properties
PublikacjaA psychrotrophic bacterium producing a cold-active β-galactosidase was isolated from Spitsbergen soil and classified as Arthrobacter sp. S3*. The gene encoding β-galactosidase was isolated from the genomic DNA library, sequenced, cloned, expressed in Escherichia coli, purified by ion exchange chromatography and characterized. The Arthrobaster sp. S3* β-galactosidase is a homotrimeric enzyme composed of 74,4 kDa subunits. It is...
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Safety assurance strategies for autonomous vehicles
PublikacjaAssuring safety of autonomous vehicles requires that the vehicle control system can perceive the situation in the environment and react to actions of other entities. One approach to vehicle safety assurance is based on the assumption that hazardous sequences of events should be identified during hazard analysis and then some means of hazard avoidance and mitigation, like barriers, should be designed and implemented. Another approach...
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PCR-ELISA: inexpensive alternative to quantitative PCR
PublikacjaPCR-ELISA (polymerase chain reaction-enzyme linked immunosorbent assay), a combination of PCR and ELISA methods, has been used since late 1980s. The technique is based on specially labelled DNA fragments which are captured by specific DNA sequences and detected by antibodies. The whole procedure of PCR-ELISA is divided into three steps: DNA extraction, PCR reaction and detection by ELISA. The method has been found as very specific...
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Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species
PublikacjaPurpose Fungi of Scopulariopsis and Microascus genera cause a wide range of infections, with S. brevicaulis being the most prevalent aetiological agent of mould onychomycosis. Proper identification of these pathogens requires sporulating culture, which considerably delays the diagnosis. So far, sequencing of rDNA regions of clinical isolates has produced ambiguous results due to the lack of reference sequences in publicly available...
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New Approaches for Escherichia coli Genotyping
PublikacjaEasy-to-perform, fast, and inexpensive methods of differentiation of Escherichia coli strains beyond the species level are highly required. Herein two new, original tools for genotyping of E. coli isolates are proposed. The first of the developed method, a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) test uses a highly variable fliC gene, encoding the H antigen as a molecular target. The designing...
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cDNA fingerprint from the hepatopancreatic glands of pond snails (Lymnaea stagnalis) exposed to benzo[a]pyrene
PublikacjaIdentification of differentially expressed genes that could be potentially used as biomarkers of PAH exposure of common invertebrate animal (like freshwater snail) would be a valuable resource for investigators interested in toxicology and biomonitoring of aquatic environments. Therefore, the aim of this research was to investigate effects of waterborne benzo[a]pyrene (B[a]P) exposure on mRNA expression in the pond snail’s (Lymnaea...
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Dzieła inżynierii w krajobrazie delty Wisły
PublikacjaOmówiono przeobrażenia historyczne układu hydrologicznego delty Wisły od Cypla Mątowskiego do kolejnych ujść: pierwotnie naturalnego w Wisłoujściu później w Gdańsku - Nowym Porcie, naturalnego w Gdańsku Górkach z 1840 r. oraz sztucznego w Gdańsku - Świbnie z 1895 r. Zaprezentowano budowle hydrotechniczne w Białej Górze, Piekle (relikt), Gdańskiej Głowie, Gdańsku-Przegalinie oraz przepompownie i przepusty wałowe na Nizinie Walichnowskiej....
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Detection and segmentation of moving vehicles and trains using Gaussian mixtures, shadow detection and morphological processing
PublikacjaSolution presented in this paper combines background modelling, shadow detection and morphological and temporal processing into one system responsible for detection and segmentation of moving objects recorded with a static camera. Vehicles and trains are detected based on their pixellevel difference from the continually updated background model utilizing a Gaussian mixture calculated separately for every pixel. The shadow detection...
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Computer animation system based on rough sets and fuzzy logic
PublikacjaA fuzzy logic inference system was created, based on the analysis of animated motion features. The objective of the system is to facilitate the creation of high quality animation by analyzing personalized styles contained in numerous animations. Sequences portraying a virtual character acting with a differentiating personalized style (natural or exaggerated) and various levels of fluidity were prepared and subjectively evaluated....
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Isolation and Characterization of Phenol-Degrading Psychrotolerant Yeasts
PublikacjaIn this study, the potential of selected psychrotolerant yeast strains for phenol biodegradation was studied. From 39 strains isolated from soil and water samples from Rucianka peat bog, three psychrotolerant yeast strains: A011, B021 and L012 showed the ability to phenol degradation. The result shows that all three yeast strains could degrade phenol at 500 mg l-1 and 750 mg l-1 concentration, whereas strains A011 and L012 could...
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Towards Cancer Patients Classification Using Liquid Biopsy
PublikacjaLiquid biopsy is a useful, minimally invasive diagnostic and monitoring tool for cancer disease. Yet, developing accurate methods, given the potentially large number of input features, and usually small datasets size remains very challenging. Recently, a novel feature parameterization based on the RNA-sequenced platelet data which uses the biological knowledge from the Kyoto Encyclopedia of Genes and Genomes, combined with a classifier...
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Cyclic deformation and fracture behaviour of additive manufactured maraging steel under variable-amplitude loading
PublikacjaThe cyclic deformation and fracture behaviour of 18Ni300 maraging steel produced by laser beam powder bed fusion is studied under variable-amplitude loading. The tests were conducted under fully-reversed strain-controlled conditions with a loading sequence comprising three ascending cycles and three descending cycles repeated sequentially until failure. After the tests, fracture surfaces were examined using height and volume surface...