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Wyniki wyszukiwania dla: s. aureus
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Bee Products and Essential Oils as Alternative Agents for Treatment of Infections Caused by S. aureus
PublikacjaBacteria of the genus Staphylococcus are important human and veterinary pathogens. A crucial characteristic for this group of bacteria is that they can easily acquire mechanisms of antibiotic resistance for a plethora of antibiotics currently in use for human and animal therapies. Therefore, there is a great need to find novel, non-antibiotic chemotherapeutics with marked antistaphylococcal activity. Promising but still underestimated...
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Untargeted Lipidomics Reveals Differences in the Lipid Pattern among Clinical Isolates of Staphylococcus aureus Resistant and Sensitive to Antibiotics
PublikacjaStaphylococcus aureus resistance to antibiotics is a significant clinical problem worldwide. In this study, an untargeted lipidomics approach was used to compare the lipid fingerprints of S. aureus clinical isolates that are resistant and sensitive to antibiotics. High-performance liquid chromatography coupled with time-of-flight mass spectrometry was employed to rapidly and comprehensively analyze bacterial lipids. Chemometric...
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Lipidomics of Staphylococcus aureus – a new insight into the antibiotic resistant phenotype
PublikacjaStaphylococcus aureus is an aggressive pathogen responsible for a variety of diseases, in cluding life-threatening sepsis. Many strains of these bacteria are resistant to multiple classes of antibiotics, which is a substantial clinical problem in the treatment of S. aureus infections. We developed a comprehensive untargeted lipidomic workflow, including sample preparation, liquid chromatography-quadrupole-time-of-flight mass spectrometry...
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Comprehensive methodology for Staphylococcus aureus lipidomics by liquid chromatography and quadrupole time-of-flight mass spectrometry
PublikacjaStaphylococcus aureus is a common pathogen known to cause relatively minor infections as well as severe disorders in humans. Although there is fair amount of published data concerning various aspects of its biology, epidemiology, genetics, etc., there is still a scarce amount of data presenting reliable and thorough investigations regarding high-throughput analysis of total S. aureus lipid content. Therefore, the aim of this study...
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Bee Bread Exhibits Higher Antimicrobial Potential Compared to Bee Pollen
PublikacjaThis study aimed at investigation of the antimicrobial potential of ethanolic extracts of bee bread (BB) and bee pollen (BP) and suspensions of these products in MHB (Mueller Hinton Broth). We covered 30 samples of BP and 19 samples of BB harvested in Polish apiaries. Slightly lower activity was observed against Gram-negative bacteria compared to Gram-positive staphylococci. BB extracts exhibited higher inhibitory potential with...
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Photoinactivation of Staphylococcus aureus using protoporphyrin IX: the role of haem-regulated transporter HrtA
PublikacjaLight- and photosensitiser-based antimicrobial photodynamic therapy is a very promising approach to the control of microbial infections. How the phenotypic features of a microorganism affect its response to photosensitiser-based photokilling represents an area of substantial research interest. To understand the mechanisms governing the phenomenon of a strain-dependent response to photodynamic inactivation (PDI), we analysed the...
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A five-year retrospective study shows increasing rates of antimicrobial drug resistance in Cabo Verde for both Staphylococcus aureus and Escherichia coli
PublikacjaObjectives Data on baseline drug resistance is important in informing future antimicrobial stewardship programs. So far, no data on the antimicrobial drug resistance of clinical isolates was available for the African archipelago of Cabo Verde. Methods We have performed a retrospective analysis over five-years (2013-17) of the antimicrombial drug susceptibility profiles of clinical isolates in the two main hospitals of Cabo Verde....
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Anthra[1,2-d][1,2,3]triazine-4,7,12(3H)-triones as a New Class of Antistaphylococcal Agents: Synthesis and Biological Evaluation
PublikacjaThe development and spread of resistance of human pathogenic bacteria to the action of commonly used antibacterial drugs is one of the key problems in modern medicine. One of the especially dangerous and easily developing antibiotic resistant bacterial species is Staphylococcus aureus. Anthra[1,2-d][1,2,3]triazine-4,7,12(3H)-triones 22–38 have been developed as novel effective antistaphylococcal agents. These compounds have been...
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MutS3: a MutS homologue of unknown biological function
PublikacjaThe homologues of MutS proteins are widespread among both Prokaryotes and Eukaryotes. MutS designated as MutS1 is a part of MMR (mismatch repair) system which is responsible for removal of mispaired bases and small insertion/deletion loops in DNA. Initially, the only MutS homologues known were those engaged in mismatch repair and these were later designated as MutS1. Subsequently, the MutS2 homologue was distinguished. MutS2 does...
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Chitosan-Based Membranes as Gentamicin Carriers for Biomedical Applications — Influence of Chitosan Molecular Weight
PublikacjaOver the past decade, much attention has been paid to chitosan as a potential drug carrier because of its non-toxicity, biocompatibility, biodegradability and antibacterial properties. The effect of various chitosan characteristics on its ability to carry different antibiotics is discussed in the literature. In this work, we evaluated the influence of the different molecular weights of this polymer on its potential as an antibacterial...
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Evaluating the antibacterial activity of muramyl dipeptide derivatives, retro-tuftsin derivatives, and anthraquinone oligopeptides against a range of pathogenic bacteria
PublikacjaSearch for new and efficient antibiotic is crucial because of microbial drug resistance and problems with side effects of the administered medication. In this study, we evaluate the in vitro microbiological activity of muramyl dipeptide derivatives, retro-tuftsin derivatives (i.e., tuftsin with reversed amino acid sequences), and combinations of retro-tuftsin derivatives with substituted anthraquinones. The potency of the investigated...
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Antibiotic-Based Conjugates Containing Antimicrobial HLopt2 Peptide: Design, Synthesis, Antimicrobial and Cytotoxic Activities
PublikacjaRecent studies have shown that modified human lactoferrin 20−31 fragment, named HLopt2, possesses antibacterial and antifungal activity. Thus, we decided to synthesize and evaluate the biological activity of a series of conjugates based on this peptide and one of the antimicrobials with proven antibacterial (ciprofloxacin, CIP, and levofloxacin, LVX) or antifungal (fluconazole, FLC) activity. The drugs were covalently connected...
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Biofilm formation and its regulation in Staphylococcus aureus
PublikacjaInfections caused by the major human and animal pathogen, Staphylococcus aureus are becoming an important problem due to its emerging resistance mechanisms and variety of virulence factors. Biofilm formation is considered to be one of the most important factor attributed to high pathogenicity of S. aureus. Described in the literature methods used in identification of biofilm forming abilities of bacteria consist of phenotypic and...
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Isolation of Bacteriocin-producing Staphylococcus spp. Strains from Human Skin Wounds, Soft Tissue Infections and Bovine Mastitis
PublikacjaA collection of 206 Staphylococcus spp. isolates was investigated for their ability to produce compounds exhibiting antistaphylococcal activity. This group included Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus xylosus strains recovered from bovine mastitis (n = 158) and human skin wounds and soft tissues infections (n = 48). Production of substances with antimicrobial activity was observed in six strains. Five...
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The assessment of microbiological antimicrobial properties of PE film loaded with nanozinc filler
Dane BadawczeThe dataset contains the results of a single series of determinations of the antimicrobial properties against E. coli and S. aureus of polyethylene films containing the nanozinc filler.
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Lack of correlation between X region spa polymorphism and virulence of methicillin resistant and methicillin sensitive Staphylococcus aureus strains
PublikacjaW celu sprawdzenia zależności pomiędzy liczbą powtórzeń 24 nukleotydowej sekwencji w fragmencie X genu spa a wirulencją gronkowców przebadano populację 176 klinicznych izolatów bakterii S. aureus. 94 z nich wykazywały oporność na metycylinę (MRSA) pozostałe 82 izolaty były wrażliwe na działanie tego antybiotyku (MSSA). W obydwu grupach nie udało się potwierdzić korelacji pomiędzy wielkością amplifikowanego fragmentu - liczbą powtórzeń...
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Biological and antibacterial properties of chitosan-based coatings with AgNPs and CuNPs obtained on oxidized Ti13Zr13Nb titanium alloy
PublikacjaDespite numerous studies, the antibacterial efficiency and cytotoxicity of chitosan-based coatings with nanometals need further studies. The purpose of this research was to determine the biological properties of chitosan coatings implemented with either silver nanoparticles (AgNPs) or copper nanoparticles (CuNPs), that were electrophoretically deposited on electrochemically oxidized Ti13Zr13Nb alloy. The SEM tests were used to...
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The Anti-Staphylococcal Potential of Ethanolic Polish Propolis Extracts
PublikacjaAbstract: The principal objective of this study was to determine the anti-staphylococcal potential of ethanol extracts of propolis (EEPs). A total of 20 samples of propolis collected from apiaries located in different regions of Poland were used in the study. The two-fold broth microdilution method revealed some important differences in the antimicrobial activity of investigated EEPs. Up to the concentration of 4096 µg/mL no activity...
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Osteoblast and bacterial cell response on RGD peptide‐functionalized chitosan coatings electrophoretically deposited from different suspensions on Ti13Nb13Zr alloy
PublikacjaMetallic materials for long-term load-bearing implants still do not provide high antimicrobial activity while maintaining strong compatibility with bone cells. This study aimed to modify the surface of Ti13Nb13Zr alloy by electrophoretic deposition of a chitosan coating with a covalently attached Arg-Gly-Asp (RGD) peptide. The suspensions for coating deposition were prepared in two different ways either using hydroxyacetic acid...
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Anticancer and antimicrobial properties of novel η6-p-cymene ruthenium(ii) complexes containing a N,S-type ligand, their structural and theoretical characterization
PublikacjaRuthenium(II) complexes are lately of great scientific interest due to their chemotherapeutic potential asanticancer and antimicrobial agents. Here we present the synthesis of new pyrazole carbothioamidederivatives and their four arene–ruthenium complexes. The title compounds were characterized with theapplication of IR, NMR, mass spectrometry, elemental analysis and X-ray diffraction. Additionally, for newcomplexes DFT calculations...
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Test of the antimicrobial properties against Staphylococcus aureus of the innovative CS-2a preservative.
Dane BadawczeThe dataset contains the results of a single series of determinations of the antimicrobial properties against Staphylococcus aureus of the innovative CS-2 a preservative in the solution of model fluids.During the test, the infected product is inoculated (on Baird-Parker Agar) at specified intervals (0 min. and after 1 day-24 h of incubation ). This...
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Evaluation of antimicrobial activities of powdered cuttlebone against Klebsiella oxytoca, Staphylococcus aureus, and Aspergillus flavus
PublikacjaBackground: The presence of medicines in the environment is considered as a serious threat to the human health. The entrance of these substances into the water sources causes soil pollution, which eventually leads to the environmental pollution and it creates some problems for the public health. Also, increasing antibiotic resistant bacteria has attracted the attention of researchers to the use of natural resources such as marine...
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Long-Period Gratings and Microcavity In-Line Mach Zehnder Interferometers as Highly Sensitive Optical Fiber Platforms for Bacteria Sensing
PublikacjaSelected optical fiber sensors offer extraordinary sensitivity to changes in external refractive (RI), which make them promising for label-free biosensing. In this work the most sensitive ones, namely long-period gratings working at (DTP-LPG) and micro-cavity in-line Mach-Zehnder interferometers (µIMZI) are discussed for application in bacteria sensing. We describe their working principles and RI sensitivity when operating in water...
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Comparison of disk and tube methods for estimation of biostatic properties of polyurethanes based on synthetic poly[(R,S)-3-hydroxybutyrate
PublikacjaThe influence of novel aliphatic polyurethanes on growth of Staphyloccocus aureus (SA)in the around of polymer samples has been estimated by disk method. It was staed that the growth of SA was inhibited.The influence of polyurethanes on planctonic Staphyloccocus aureus was also pefrormed by tube method. In this test the slight decrease of bacteria concentration was noticed.
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Protective effect of lysostaphin from Staphylococcus simulans against growth of Staphylococcus aureus in milk and some other food products
PublikacjaZbadano skuteczność hamowania rozwoju Staphylococcus aureus w mleku, mięsie wieprzowym i majonezie lizostafyną uzyskaną przez ekspresję genu enzymu ze Staphylococcus simulans w komórkach Escherichia coli. Przeprowadzone eksperymenty wykazały znaczący wpływ lizostafyny na ograniczenie rozwoju Staphylococcus aureus.
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Wpływ poliuretanów z syntetycznym polihydroksymaślanem na wzrost mikroorganizmów
PublikacjaW pracy przedstawiono wyniki badań oddziaływania nowych alifatycznych poliuretanów, otrzymanych z udziałem polihydroksymaślanu na wzrost Staphylococcus aureus, Escherichia coli i Candida albicans wokół próbek poliuretanowych. Najbardziej wrażliwym na działanie materiału poliuretanowego w warunkach prowadzenia doświadczenia był szczep Staphylococcus aureus.
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Biofilm Production and Presence of ica and bap Genes in Staphylococcus aureus Strains Isolated from Cows with Mastitis in the Eastern Poland
PublikacjaThe aim of the study was phenotypic and genotypic analysis of 132 S. aureus strains isolated from mastitis in eastern Poland in respect to their biofilm formation ability. The analysis of the size polymorphism of fragment X in the gene encoding protein A (spa) revealed high genetic differentiation of the analyzed group of isolates. The ability of biofilm formation by the isolates was tested using two phenotypic methods. The Congo...
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Zakażenia bakteryjne w przewlekłym zapaleniu ucha środkowego - ocena przydatności diagnostyki molekularnej opartej o technikę PCR. Bacterial infection in chronic otitis media - assessment of usefulness of molecular diagnostic based in PCR method.
PublikacjaIdentyfikacja zakażeń w przewlekłym zapaleniu ucha środkowego jest trudna, o czym świadczą wysokie odsetki ujemnych wyników badań bakteriologicznych. Przyczyną tego jest trudność w uzyskiwaniu wiarygodnego materiału do badań oraz stosunkowo niska czułość klasycznych metod diagnostycznych. Amplifikacja bakteryjnego DNA techniką PCR jest badaniem, które może przyczynić się do zwiększenia efektywności i czułości badań bakteriologicznych....
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Bovine Serum Albumin – Hydroxyapatite Nanoflowers as Potential Local Drug Delivery System of Ciprofloxacin
PublikacjaIntroduction: Hybrid nanoflowers are structures consisting of organic (enzymes, proteins, nucleic acids) and inorganic components (mostly metal phosphates) with a flower-like hierarchical structure. Novel hybrid nanoflowers based on bovine serum albumin (BSA) and hydroxyapatite (HA) were obtained and characterized. Study on BSA-HA nanoflowers as potential drug delivery system is reported for the first time. Methods: Embedding ciprofloxacin...
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Evaluation of antimicrobial properties of thermomelt adhesives modified with zinc compounds
Dane BadawczeThe dataset contains the results of microbiological tests of thermomelt adhesives modified with zinc compounds, whose activity was assessed for their ability to reduce the number of Escherichia coli and Staphylococcus aureus strain, representing the Gram (-) and Gram (+) bacteria, respectively. The evaluation of antimicrobial properties of samples were...
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Bacteriophages as an antistaphylococcal agent
PublikacjaRecently observed increase of infection caused by Staphylococcus aureus is currently one of the most important problem of clinical microbiology. Most of the strains responsible for the infections are a MRSA (methicillin resistant Staphylococcus aureus) strains. In this situation commonly used antibiotics are not sufficient. There is an urgent need to search for a new nonatibiotic treatment against this pathogen. In the literature...
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Comparative Analysis of Metabolic Variations, Antioxidant Profiles and Antimicrobial Activity of Salvia hispanica (Chia) Seed, Sprout, Leaf, Flower, Root and Herb Extracts
PublikacjaThe purpose of this study was to evaluate the phytochemical profiles of the seeds, sprouts, leaves, flowers, roots and herb of Salvia hispanica and to demonstrate their significant contribution to antioxidant and antimicrobial activities. Applied methods were: HPLC-DAD coupled with post-column derivatization with ABTS reagent, untargeted metabolomics performed by LC-Q-Orbitrap HRMS, and two-fold micro-dilution broth method, which...
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ADSRRS-fingerprinting and PCR MP techniques for studies of intraspecies genetic relatedness in Staphylococcus aureus
PublikacjaW pracy pokazano użyteczność dwóch nowych metod do typowania molekularnego Staphylococcus aureus, ADSRRS-fingerprinting (Amplification of DNA fragments Surrounding Rare Restriction Sites) i PCR MP (PCR melting profile). Przebadano 37 szczepów Staphylococcus aureus izolowanych od pacjentów z czyracznością. Jako metodę referencyjną zastosowano REA-PFGE. Okazało się, że izolaty Staphylococcus aureus z nosa od niektórych pacjentów...
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A novel method of creating thermoplastic chitosan blends to produce cell scaffolds by FDM additive manufacturing
PublikacjaDue to its remarkable and promising biological and structural properties, chitosan has been widely studied in several potential applications in the biomedical sector. Attempts are being made to use this polymer and its properties in thermoplastics dedicated to 3D printing in FDM technology. However, chitosan can be processed only from acid solution, which limits its applications. The paper presents a new path for the production...
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Composite Polyurethane-Polylactide (PUR/PLA) Flexible Filaments for 3D Fused Filament Fabrication (FFF) of Antibacterial Wound Dressings for Skin Regeneration
Publikacjahis paper addresses the potential application of flexible thermoplastic polyurethane (TPU) and poly(lactic acid) (PLA) compositions as a material for the production of antibacterial wound dressings using the Fused Filament Fabrication (FFF) 3D printing method. On the market, there are medical-grade polyurethane filaments available, but few of them have properties required for the fabrication of wound dressings, such as flexibility...
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Paenibacillus alvei MP1 as a Producer of the Proteinaceous Compound with Activity against Important Human Pathogens, Including Staphylococcus aureus and Listeria monocytogenes
PublikacjaAn emerging need for new classes of antibiotics is, on the one hand, evident as antimicrobial resistance continues to rise. On the other hand, the awareness of the pros and cons of chemically synthesized compounds’ extensive use leads to a search for new metabolites in already known reservoirs. Previous research showed that Paenibacillus strain (P. alvei MP1) recovered from a buckwheat honey sample presented a wide spectrum of...
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Fish gelatin films containing aqueous extracts from phenolic-rich fruit pomace
PublikacjaThe aim of the work was to study the feasibility of using aqueous extracts from rowanberry, blue-berried honeysuckle, and chokeberry pomace for the formulation of fish gelatin films with antioxidant and antimicrobial activity as well as improved mechanical and water barrier properties. The predominant phenolic components in rowanberry and chokeberry extracts were hydroxycinnamates, and in blue-berried honeysuckle extract antho cyanins. Although...
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Peptidoglycan hydrolases - potential weapons against Staphylococcus aureus
PublikacjaBacteria of the genus Staphylococcus are common pathogens responsible for a broad spectrum of human and animal infections and belong to most important etiological factors causing food poisoning. Because of rapid increase in prevalence of isolation of staphylococci resistant to many antibiotics, there is an urgent need for development of new alternative chemotherapeutics. A number of studies have recently demonstrated the strong...
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Fish gelatin-nanoclay films. Part I: Effect of a kind of nanoclays and glycerol concentration on mechanical and water barrier properties of nanocomposites
PublikacjaThe aim of work was the improvement of the mechanical and water barrier properties of nanocomposites prepared from fish gelatin and nanoclays (5–15%), plasticized with glycerol at different concentrations. The effect of hydrophilic Cloisite Na1 and Nanomer®PGV was compared with that exerted by organically modified more hydrophobic Nanofil®2 and NanoBent ZR-1. Antimicrobial activity of nanocomposites containing hydrophobic nanoclays...
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Glucose oxidase as an important yet overlooked factor determiningthe antibacterial activity of bee pollen and bee bread
PublikacjaBee pollen (BP) and bee bread (BB) have attracted great attention due to their biological activities including antibacterial activity. However, the mechanism of antibacterial activity is largely unknown. Therefore, we aimed to characterise the antibacterial effect of BP and BB aqueous extracts against bacterial pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis and Enterococcus faecalis)...
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Comparative Assessment of Bacteriophage and Antibiotic Activity against Multidrug-Resistant Staphylococcus aureus Biofilms
PublikacjaProblems connected with biofilm-related infections and antibiotic resistance necessitate the investigation and development of novel treatment strategies. Given their unique characteristics, one of the most promising alternatives to conventional antibiotics are bacteriophages. In the in vitro and in vivo larva model study, we demonstrate that phages vB_SauM-A, vB_SauM-C, and vB_SauM-D are effective antibiofilm agents. The exposure...
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Badanie właściwości fotokatalitycznych, biobójczych i magnetycznych tlenku tytanu(IV) modyfikowanego nanocząstkami monoi bimetalicznymi Pt, Ag, Cu, Au oraz Pd
PublikacjaZakres badań obejmował otrzymywanie i charakterystykę nanokompozytów tlenku tytanu(IV) modyfikowanego mono- oraz bimetalicznymi nanocząstkami metali platyny, palladu, złota, srebra oraz miedzi. Zbadano wpływ zawartości metali, matrycy TiO2 oraz połączeniami metali na właściwości fotokatalityczne, biobójcze i magnetyczne otrzymanych nanokompozytów TiO2. Aktywność fotokatalityczną zbadano w reakcji degradacji modelowych związków oraz...
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Inhibitory and antimicrobial activities of OGTI and HV-BBI peptides, fragments and analogs derived from amphibian skin
PublikacjaA series of linear and cyclic fragments and analogs of two peptides (OGTI and HV-BBI) isolated from skin secretions of frogs were synthesized by the solid-phase method. Their inhibitory activity against several serine proteinases: bovine beta-trypsin, bovine alpha-chymotypsin, human leukocyte elastase and cathepsin G from human neutrophils, was investigated together with evaluation of their antimicrobial activities against Gram-negative...
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Preparation of Ag/Cu-doped titanium (IV) oxide nanoparticles in W/O microemulsion
PublikacjaNanocząstki Cu-TiO2 oraz Ag/Cu-TiO2 otrzymano w mikroemulsji W/O. Właściwości fotokatalityczne zbadano w procesie degradacji fenolu (0,21mM) w zakresie światła widzialnego. Właściwości biobójcze zbadano poprzez wyznaczenie minimalnego stężenia zahamowania wzrostu (MIC) na szczepach bakterii E. coli, St. aureus oraz grzybów S. Cerevisiae i z rodziny Candida. Dla otrzymanych fotokatalizatorów określono wpływ stężenia domieszki metali...
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Molecular typing of Staphylococcus aureus isolated from cows with mastitis in the east of Poland on the basis of polymorphism of genes coding protein A and coagulase
PublikacjaPolimorfizm genów kodujących białko A i koagulazę wykorzystano do badania zmienności genetycznej bakterii Staphylococcus aureus wyizolowanych z gruczołów mlekowych krów u których stwierdzono stan zapalny. W badanej grupie 82 szczepów wyróżniono 24 genotypy.
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The Antimicrobial Potential of Bacteria Isolated from Honey Samples Produced in the Apiaries Located in Pomeranian Voivodeship in Northern Poland
PublikacjaThe principal objective of this study was to determine whether the honeys produced in apiaries located in Pomeranian Voivodeship (Northern Poland) contain bacteria producing metabolites with growth inhibition potential against important human and animal pathogens. The pathogens included Staphylococcus aurues, Staphyloccocus epidermidis, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, and Candida albicans. From...
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Synteza nowych aminokwasowych i peptydowych analogów kwasu mykofenolowego oraz ich aktywność biologiczna i mikrobiologiczna
PublikacjaKwas mykofenolowy (MPA) oraz jego dwie stosowane klinicznie pochodne: mykofenolan sodu (MPS, CellCept) i mykofenolan mofetylu (MMF, Myfortic) są jednymi z najczęściej używanych leków hamujących proliferację limfocytów. Obok aktywności immunosupresyjnej, farmaceutyk wykazuje również właściwości przeciwbakteryjne, przeciwgrzybicze, przeciwwirusowe a także przeciwnowotworowe. W ramach realizacji swojej pracy doktorskiej zaprojektowałam...
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Synthesis and antimicrobial activity of amino acid and peptide derivatives of mycophenolic acid
PublikacjaThe series of 16 novel amino acid and peptide mycophenolic acid (MPA) derivatives was obtained as potential antibacterial agents. Coupling of MPA with respective amines was optimized with condensing reagents such as EDCI/DMAP and T3P/TEA. Amino acid analogs were received both as methyl esters and also with the free carboxylic group. The biological activity of the products was tested on five references bacterial strains: Klebsiella...
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Thermosensitive composite based on agarose and chitosan saturated with carbon dioxide. Preliminary study of requirements for production of new CSAG bioink.
PublikacjaThis study introduces a method for producing printable, thermosensitive bioink formulated from agarose (AG) and carbon dioxide-saturated chitosan (CS) hydrogels. The research identified medium molecular weight chitosan as optimal for bioink production, with a preferred chitosan hydrogel content of 40–60 %. Rheological analysis reveals the bioink's pseudoplastic behavior and a sol-gel phase transition between 27.0 and 31.5 °C. The...
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The Synergistic Microbiological Effects of Industrial Produced Packaging Polyethylene Films Incorporated with Zinc Nanoparticles
PublikacjaZinc compounds in polyolefin films regulate the transmission of UV-VIS radiation, affect mechanical properties and antimicrobial activity. According to hypothesis, the use of zinc- containing masterbatches in polyethylene films (PE) with different chemical nature—hydrophilic zinc oxide (ZO) and hydrophobic zinc stearate (ZS)—can cause a synergistic effect, especially due to their antimicrobial properties. PE films obtained on an...
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Hydrazinolysis Products of Selected Sugar Lactones—Crystal Structure and Microbiological Activity
PublikacjaCommercially available lactones, as well as those synthesized by us, turned out to be good substrates for the synthesis of sugar hydrazides. The exception was L-ascorbic acid, whose hydrazinolysis led to the formation of a hydrazinium salt, not the hydrazide as expected. The structure of all compounds was confirmed by NMR and X-ray analyses. The lower durability of hydrazinium L-ascorbate was additionally confirmed by thermogravimetric...
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Virulence gene profiles in Staphylococcus aureus isolated from cows with subclinical mastitis in eastern Poland
PublikacjaStaphylococcus aureus is arguably the most important pathogen involved in bovine mastitis. The aim of this study was to determine the virulence gene profiles of 124 Staph. aureus isolates from subclinical mastitis in cows in eastern Poland. The presence of 30 virulence genes encoding adhesins, proteases and superantigenic toxins was investigated by PCR. The 17 different combinations of adhesin genes were identified. Occurrence...
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Antibacterial activity of gelatin-based films incorporated with chitosan or silver
PublikacjaFolie żelatynowe z dodatkiem srebra jonowego powodowały redukcję komórek Escherichia coli i Staphylococcus aureus o 90% pod powierzchnią folii. Nie obserwowano natomiast strefy zahamowania wzrostu dookoła krążków folii. W przypadku filmów chitozanowych lub żelatynowo-chitozanowych ich przeciwdrobnoustrojowa aktywność zależała od stopnia deacetylacji chitozanu.
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Chitosan and polyvinyl alcohol nanocomposites with cellulose nanofibers from ginger rhizomes and its antimicrobial activities
PublikacjaThe inhibitory effect of 5% GNF bionanocomposites against Bacillus cereus, Escherichia coli, Staphylococcus aureus and Salmonella typhimurium indicated good antibacterial activity of the nanocomposites due to the addition of GNF in the biopolymer matrices. The use of GNF will help to increase the economic values of agricultural waste and the characteristic properties of GNF derived bionanocomposites could be possibly used in medical...
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Draft genome sequence of antimicrobial producing Paenibacillus alvei strain MP1 reveals putative novel antimicrobials
PublikacjaA Paenibacillus strain isolated in previous research exhibited antimicrobial activity against relevant human pathogens including Staphylococcus aureus and Listeria monocytogenes. In this study, the genome of the aforementioned strain, designated as MP1, was shotgun sequenced. The draft genome of strain MP1 was subject to multiple genomic analyses to taxonomically characterize it and identify the genes potentially responsible for...
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Essential oils as potential anti-staphylococcal agents
PublikacjaAntibiotic therapy of staphylococcal mastitis is characterized by significantly lower cure rates compared to infections caused by other microorganisms. Thus, it is necessary to search for new, alternative, non-antibiotic agents that are effective in the eradication of these bacteria. The aim of our research was to investigate the antimicrobial, especially anti-staphylococcal potential of a large collection (n=36) of essential oils...
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Synthesis, Characterization and Biological Investigations of Half-Sandwich Ruthenium(II) Complexes Containing Benzimidazole Moiety
PublikacjaHalf-sandwich Ru(II) complexes belong to group of biologically active metallo-compounds with promising antimicrobial and anticancer activity. Herein, we report the synthesis and characteri- zation of arene ruthenium complexes containing benzimidazole moiety, namely, [(η6-p-cymene)RuC l(bimCOO)] (1) and [(η6-p-cymene)RuCl2(bim)] (2) (where bimCOO = benzimidazole-2-carboxylate and bim = 1-H-benzimidazole). The compounds were characterized...
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Otzrymywanie i charakterystyka nowych biomateriałów o aktywności przeciwdrobnoustrojowej na bazie chitozanu, kolagenu i żelatyny
PublikacjaW niniejszej pracy przedstawiono opis wyników badań poświęconych otrzymywaniu i charakterystyce biomateriałów zbudowanych z chitozanu, kolagenu i żelatyny, o strukturze i właściwościach wymaganych od nowoczesnego materiału opatrunkowego, stanowiącego jednocześnie dogodną matrycę do inkorporacji związków biologicznie czynnych, zwłaszcza lizostafyny. Opracowane materiały stanowią także próbę odpowiedzi na potrzeby medyczne dotyczące...
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Effect of lysozyme or nisin on survival of some bacteria treated with high pressure at subzero temperature
PublikacjaCelem pracy było określenie wrażliwości gramujemnych i gramdodatnich bakterii na działanie ciśnienia 193MPa w temp. -20°C w obecności lizozymu lub nizyny. Stwierdzono, że połączone działanie ciśnienia i badanych substancji przeciwdrobnoustrojowych na zawiesiny bakterii Escherichia coli i Staphylococcus aureus powodowało większy stopień inaktywacji komórek niż suma efektów wywieranych przez pojedyncze czynniki.
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Synthesis of isomeric, oxathiolone fuded chalcones and comparison of their activity toward various microorganisms and human cancer cell lines
PublikacjaIzomeryczne oksationolowe sprzężone chalkony otrzymano w wyniku kondensacji odpowiedniego acetylobenzo[1,3]oksatiolo-2-onu z aldehydem benzoesowym w środowisku kwaśnym. Określono aktywność cytotoksyczną otrzymanych związków wobec komórek linii HeLa, przeciwbakteryjną wobec Micrococcus luteus, Staphylococcus aureus, Salmonella typhimurium, Escherichia coli i Proteus vulgaris, przeciwgrzybową wobec Candida albicans i tuberkulostatyczną...
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Acid-catalysed synthesis of oxathiolone fused chalkones. Comparison of their activity toward various microrganisms and human cancer lines
PublikacjaOtrzymano podstawione pochodne oksationolowych sprzężonych chalkonów w wyniku kondensacji 4-acetylo-5-metoksy-2-0ksobenz[1,3]oksatiolu z aldehydem benzoesowym w środowisku kwaśnym. Badano właściwości biologiczne otrzymanych związków. Trzy z otrzymanych pochodnych wykazały umiarkowaną aktywność wobec komórek HeLa, dwa były aktywne wobec Micrococcus luteus i Staphylococcus aureus, a jeden wobec Mycobacterium tuberculosis.
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Oxolane Ammonium Salts (Muscarine-Like)—Synthesis and Microbiological Activity
PublikacjaCommercially available 2-deoxy-D-ribose was used to synthesize the appropriate oxolane derivative—(2R,3S)-2-(hydroxymethyl)oxolan-3-ol—by reduction and dehydration/cyclization in an acidic aqueous solution. Its monotosyl derivative, as a result of the quaternization reaction, allowed us to obtain eight new muscarine-type derivatives containing a quaternary nitrogen atom and a hydroxyl group linked to the oxolane ring. Their structure...
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The combined effect of moderate pressure and chitosan on Escherichia coli and Staphylococcus aureus cells suspended in a buffer and on natural microflora of apple juice and minced pork
PublikacjaW pracy określono przeżywalność bakterii Escherichia coli i Staphylococcus aureus oraz naturalnej mikroflory soku jabłkowego i rozdrobnionego mięsa świńskiego po działaniu ciśnienia 193 MPa w temp. -20 C w obecności chitozanu. Stwierdzono, że traktowanie mikroorganizmów ciśnieniem w obecności chitozanu pozwala na przedłużenie trwałości badanych produktów przechowywanych w temperaturze chłodniczej o około 8-10 dni.
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Successive cytotoxicity control by evolutionary surface decorated electronic push-pull green ZnCr-LDH nanostructures: Drug delivery enlargement for targeted breast cancer chemotherapy
PublikacjaThe reason for the increasing bioavailability and biocompatibility of the porous nanomaterials in the presence of different (bio)molecules is still unknown. The role of difference functional groups and their interactions with the potential bioavailability and biocompatibility is of great importance. To investigate the potential contribution of the electronic effects (especially on the surface of the porous nanomaterials) on their...
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Antimicrobial properties of chitosan solutions, chitosan films and gelatin-chitosan films
PublikacjaThe antimicrobial properties of chitosan solutions and films against selected bacteria and the effect of chitosan incorporation into gelatin films were studied. The bactericidal effect of chitosan solutions increased with time and temperature of sample incubation. Two psychrotrophic strains Pseudomonas fluorescens and Listeria innocua were more sensitive to chitosan than mesophilic strains Escherichia coli and Staphylococcus aureus....
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Evaluation of antimicrobial activity of porous composites based on chitosan/poly (vinyl alcohol)
Dane BadawczeThe dataset contains the results of microbiological tests of composite porous materials whose activity was assessed for their ability to reduce the number of Escherichia coli and Staphylococcus aureus strain, representing the Gram (-) and Gram (+) bacteria, respectively.
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Bee Pollen and Bee Bread as a Source of Bacteria Producing Antimicrobials
PublikacjaThe principal objective of the study was the isolation and identification of bacteria that are present in mature bee bread (BB) and dried (ready for selling and consumption) bee pollen (BP). Obtained isolates were screened for their potential to inhibit select human pathogenic bacteria and their ability to produce enzymes of particular industrial importance. Four and five samples of BP and BB, respectively, were used for the study....
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Preparation, characterization and antimicrobial activity of polyvinyl alcohol/gum arabic/chitosan composite films incorporated with black pepper essential oil and ginger essential oil
PublikacjaIn this study, biocomposite films based on polyvinyl alcohol (PVA), gum arabic (GA) and chitosan (CS) incorporated with BPEO and GEO were fabricated by solvent casting method. FTIR, XRD, SEM and DSC were performed with mechanical and antimicrobial properties of PVA/GA/CS films with and without BPEO and GEO. The BPEO and GEO incorporated PVA/GA/CS films were significantly inhibited the growth of Bacillus cereus, Staphylococcus aureus,...
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Synthesis and Characterization of Monometallic (Ag, Cu) and Bimetallic Ag-Cu Particles for Antibacterial and Antifungal Applications
PublikacjaIn this paper, the experimental studies are concerned with the effect of the synthesis parameters on the formation of monometallic Ag and Cu nanoparticles (NPs). We consider the synthesis strategies verification for the bimetallic core-shell and alloy particles preparation. It was successfully obtained by chemical reduction method. The obtained colloidal solution is characterized by the transmission electron microscopy (TEM) with...
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Effect of pH and high pressure at sub-zero temperature on the viability of some bacteria
PublikacjaCelem badań było określenie przeżywalności Escherichia coli i Staphylococcus aureus zawieszonych w soku jabłkowym (pH 3,8) po działaniu ciśnienia 193MPa w temperaturze -20°C. Stwierdzono, że bezpośrednio po działaniu ciśnienia przeżywalność badanych szczepów bakterii nie zmieniała się znacząco. Jednakże liczba komórek zmniejszała się z czasem przechowywania ciśnieniowanych prób. Żywe komórki mikroorganizmów nie były wykrywane w...
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Organizmy obecne w osadzie dennym basenów kąpielowych
PublikacjaMonitorowanie parametrów wody basenowej jest istotne z uwagi na zdrowie osób korzystających z basenu jak i pracowników obsługi. Istnieją trzy źródła materii organicznej: woda dostarczona do basenu, pasywne zanieczyszczenia wnoszone przez osoby kąpiące się oraz zanieczyszczenia organiczne w postaci moczu i kału. Jakość wody w basenach powinna być porównywalna do jakości wody pitnej. Jakość mikrobiologiczna opiera się na tradycyjnych...
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The Creation of an Antimicrobial Coating on Contact Lenses by The Use of Nanocopper
PublikacjaThe aim of the research was to creation an antimicrobial coating on contact lens and examine the ability of copper nanoparticles to decrease microbial adhesion and prevent the growth of bacteria. The creation was based on the immersion method in solution with dispersed nanoparticles of copper at concentration 200 pm. There were evaluated follows factors: time of immersion and base to dispersed nanoparticles. Three solutions: ethyl...
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In vitro biodegradation of bacterial nanocellulose under conditions simulating human plasma in the presence of selected pathogenic microorganisms
PublikacjaThe Biodegradability of bacterial nanocellulose (BNC) was assessed based on a change in its properties under conditions simulating human plasma in the presence or absence of the pathogens Staphylococcus aureus, Candida albicans and Aspergillus fumigatus. It was shown that the dry mass of BNC did not change during 6 months of incubation, except for samples stored in the presence of moulds, where the dry mass reduced by 40 %. The...
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Synthesis, Antimicrobial and Mutagenic Activity of a New Class of d-Xylopyranosides
PublikacjaEight N-[2-(2′,3′,4′-tri-O-acetyl-α/β-d-xylopyranosyloxy)ethyl]ammonium bromides, a new class of d-xylopyranosides containing a quaternary ammonium aglycone, were obtained. Their complete structure was confirmed using NMR spectroscopy (1H, 13C, COSY and HSQC) and high-resolution mass spectrometry (HRMS). An antimicrobial activity against fungi (Candida albicans, Candida glabrata) and bacteria (Staphylococcus aureus, Escherichia...
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Use of Ginger Nanofibers for the Preparation of Cellulose Nanocomposites and Their Antimicrobial Activities
PublikacjaHere, we report, for the first time, the isolation of ginger nanofibers (GNF) from ginger rhizomes spent by acid hydrolysis and followed by high-pressure homogenization. Scanning electron microscopy was utilized to identify the surface morphology of the GNF and the widths ranged between 130 to 200 nm. Structural analysis of GNF was identified by Fourier transform infrared spectroscopy, Differential scanning calorimetry, and X-ray...
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Microwave heat treatment application to pasteurization of human milk
PublikacjaA prototype of microwave pasteurizer has been proposed as an alternative for holder pasteurization (HP) routinely used in Human Milk Bank (HMB), ensuring microbiological safety of human milk (HM). It was shown that the time of heat generation was about 15–16 min shorter by applying the microwave than in HP. Total inactivation of heat-sensitive bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus...
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The effect of UV-C irradiation on lipids and selected biologically active compounds in human milk
PublikacjaThe effect of UV-C irradiation of human milk on lipid oxidation, content of antioxidants (vitamin C and catalase, CAT) and bactericidal compounds (lysozyme), as well as the total antioxidant capacity (TAC), of the breast milk was investigated. In parallel, the extent of inactivation of some bacteria was also determined. UV-C at doses from 85 to 740 J L−1 caused total inactivation of Escherichia coli and Staphylococcus aureus, but...
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Ciprofloxacin-modified degradable hybrid polyurethane-polylactide porous scaffolds developed for potential use as an antibacterial scaffold for regeneration of skin
PublikacjaThe aim of the performed study was to fabricate an antibacterial and degradable scaffold that may be used in the field of skin regeneration. To reach the degradation criterion for the biocompatible polyurethane (PUR), obtained by using amorphous α,ω-dihydroxy(ethylene-butylene adipate) macrodiol (PEBA), was used and processed with so-called “fast-degradable” polymer polylactide (PLA) (5 or 10 wt %). To meet the antibacterial requirement...
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Structural changes of bacterial cellulose due to incubation in conditions simulating human plasma in the presence of selected pathogens
PublikacjaBacterial nanocellulose (BNC) is a natural biomaterial with a wide range of medical applications. However, it cannot be used as a biological implant of the circulatory system without checking whether it is biodegradable under human plasma conditions. This work aimed to investigate the BNC biodegradation by selected pathogens under conditions simulating human plasma. The BNC was incubated in simulated biological fluids with or...
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The Production Possibility of the Antimicrobial Filaments by Co-Extrusion of the PLA Pellet with Chitosan Powder for FDM 3D Printing Technology
PublikacjaThe last decades have witnessed a major advancement and development in three-dimensional (3D) printing technology. In the future, the trend’s utilization of 3D printing is expected to play an important role in the biomedical field. This work presents co-extrusion of the polylactic acid (PLA), its derivatives (sPLA), and chitosan with the aim of achieving filaments for printing 3D objects, such as biomedical tools or implants. The...
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The Antibacterial and Antifungal Textile Properties Functionalized by Bimetallic Nanoparticles of Ag/Cu with Different Structures
PublikacjaWe reported a preparation and characterization of five kinds of impregnation solutions, containing Ag/Cu in the formof bimetallic nanoparticles (alloy and core-shell) as well as ionic species. The cotton-polyester textiles were successfully impregnated during the washing and ironing process by as-prepared solutions to have antibacterial and antifungal properties against to Escherichia coli, Staphylococcus aureus, and Candida albicans....
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Comparison of antimicrobial activity of selected, commercially available wound dressing materials
PublikacjaOBJECTIVE: The aim of our study was to examine the antimicrobial potential of eight selected, commercially available wound dressings containing different antimicrobial agents: silver, chlorhexidine acetate, povidone-iodine, and manuka honey. METHOD: The materials were tested against four reference strains of bacteria: Staphylococcus aureus (PCM 2051), Staphylococcus epidermidis (PCM 2118), Pseudomonas aeruginosa (ATCC 27853),...
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Przydatność białka rybiego w postaci kolagenu lub żelatyny oraz polisacharydu - kapa-karagenu do wytwarzania aktywnych opakowań biodegradowalnych = Usefulness of fish collagen, gelatin and carrageenan for preparation of active biodegradable packages
PublikacjaSummary - The possibility of use of protein films made of fish collagen or gelatin as well as polysaccharide carrageenan films as carriers of enzymes (lysozyme or lysostaphyne), for preparation of microbiologically active packages, was investigated. It was found that crosslinking of such systems with N-[3(dimethylamino)propyl]-N'-ethylcarboimide (EDC) does not influence the activity of lysozyme immobilized in the films (Table 1)....
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The problem of infections associated with implants – an overview
PublikacjaImplant-associated infections are serious and relatively common complication that leads to implant loss. The purpose of this paper is to gather knowledge about this issue. A literature review of the epidemiology, risk factors and pathogenesis of infections related to implants was carried out. This position collects data on commonly used implants and infections associated with them from various fields of medicine and contains classifications...
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Antibacterial evaluation of bioactive modifiers of bone cements: antibiotics, nanometals and chitosan
PublikacjaModern biomaterials in addition to their basic tasks, can serve as carriers of active substance. The release of a bioactive particles allows to locally fight infection or its prevent it. Bone cements additionally to their basic applications in orthopedic surgery can also serve to deliver locally active substances. Currently, only antibiotics are routinely used as modifiers for bone cements. Bioactive bone cements constitute...
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The characterization of collagen‑based scaffolds modified with phenolic acids for tissue engineering application
PublikacjaThe aim of the experiment was to study the morphology of collagen-based scaffolds modified by caffeic acid, ferulic acid, and gallic acid, their swelling, and degradation rate, as well as the biological properties of scaffolds, such as antioxidant activity, hemo- and cytocompatibility, histological observation, and antibacterial properties. Scaffolds based on collagen with phenolic acid showed higher swelling rate and enzymatic...
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Antibacterial Porous Systems Based on Polylactide Loaded with Amikacin
PublikacjaThree porous matrices based on poly(lactic acid) are proposed herein for the controlled release of amikacin. The materials were fabricated by the method of spraying a surface liquid. Description is given as to the possibility of employing a modifier, such as a silica nanocarrier, for prolonging the release of amikacin, in addition to using chitosan to improve the properties of the materials, e.g., stability and sorption capacity....
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Chitosan-protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials.
PublikacjaAIMS: The development of technology for preparing chitosan-protein scaffolds loaded with lysostaphin, which potentially could be used as dressing for wound treatment and soft tissue infections caused by Staphylococcus aureus. METHODS AND RESULTS: The unique technology of chitosan solubilization using gaseous CO(2) instead of organic or inorganic acids was used for the incorporation of lysostaphin, the enzyme that exhibits bactericidal...
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Synergistic Effects of Propolis Combined with 2-Phenoxyethanol and Antipyretics on the Growth of Staphylococcus aureus
PublikacjaThe present investigation aimed to assess the combinational effect of commonly usedantipyretics and antiseptics with ethanolic extracts of propolis (EEPs) on the growth inhibition ofStaphylococcus aureus. The broth microdilution checkerboard assay revealed synergistic interactionsbetween all investigated antipyretics, namely acetylsalicylic acid, ibuprofen, and acetaminophen,with EEPs samples. The values of the fractional inhibitory...
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Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells
PublikacjaThe specific role of size scale, surface capping, and aspect ratio of zinc oxide (ZnO) particles on toxicity toward prokaryotic and eukaryotic cells was investigated. ZnO nano and microparticles of controlled size and mor- phology were synthesized by wet chemical methods. Cytotoxicity toward mammalian cells was studied using a human osteoblast cancer cell line and antibacterial activity using Gram-negative bacteria (Escherichia...
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Ionic liquid as morphology-directing agent of two-dimensional Bi2WO6: New insight into photocatalytic and antibacterial activity
PublikacjaAn efficient and durable utilization of light to drive photocatalytic reactions still requires the overcoming of barriers. Herein, two-dimensional (2D) ultrathin IL_Bi2WO6 (IL_BWO) photocatalysts were prepared for the first time via ionic liquid-assisted hydrothermal route by adjusting the amount of tetrabutylammonium chloride [TBA][Cl], synthesis temperature and duration. IL played the role of morphology-directing agent given...
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Clay-filled starch films. Part I: Effect of clay kind and glycerol concentration on functional properties of composites
PublikacjaThe aim of this work was to improve the mechanical and water barrier properties of composite filmsprepared from starch and clays, plasticized with glycerol at different concentrations. The effects ofhydrophilic Closite Naþand Nanomer PGV were compared with that exerted by organicallymodifiedmorehydrophobicNanofil2 and NanoBent ZR-1. The antimicrobial activity of compositescontaining hydrophobic clays was also investigated. The hydrophilic...
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Obtaining and Characterization of the PLA/Chitosan Foams with Antimicrobial Properties Achieved by the Emulsification Combined with the Dissolution of Chitosan by CO2 Saturation
PublikacjaA new method of obtaining functional foam material has been proposed. The materials were created by mixing the poly lactic acid (PLA) solution in chloroform, chitosan (CS) dissolved in water saturated with CO2 and polyethylene glycol (PEG), and freeze-dried for removal of the solvents. The composite foams were characterized for their structural (SEM, FT-IR, density, porosity), thermal (DSC), functional (hardness, elasticity, swelling...
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Obtaining and Characterization of the PLA/Chitosan Foams with Antimicrobial Properties Achieved by the Emulsification Combined with the Dissolution of Chitosan by CO2 Saturation
PublikacjaA new method of obtaining functional foam material has been proposed. The materials were created by mixing the poly lactic acid (PLA) solution in chloroform, chitosan (CS) dissolved in water saturated with CO2 and polyethylene glycol (PEG), and freeze-dried for removal of the solvents. The composite foams were characterized for their structural (SEM, FT-IR, density, porosity), thermal (DSC), functional (hardness, elasticity, swelling...
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Antimicrobial Activity of Honey
PublikacjaHoney has had a valued place in traditional medicine for centuries. It was used to overcome liver, cardiovascular and gastrointestinal problems and for treatment of some types of infectious disease. Particularly, good results were achieved in the case of application of this product for therapy of infected, difficult to heal wounds. The high health-promoting properties of honey have been recently confirmed in many research investigations....
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Probiotic potential of Bacillus Isolates from Polish Bee Pollen and Bee Bread
PublikacjaThe main goal of this study was the evaluation of the probiotic potential of 10 Bacillus spp. strains isolated from 5 bee bread and 3 bee pollen samples. The antagonistic interaction with Staphylococcus aureus and Escherichia coli was a primary criterion for the preliminary selection of the isolates. Three out of ten strains—PY2.3 (isolated from pollen), BP20.15 and BB10.1 (both isolated from bee bread)—were found to be possible...
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The effect of nanoparticles size on photocatalytic and antimicrobial properties of Ag-Pt/TiO2 photocatalysts
PublikacjaAg-Pt-modified TiO2 nanocomposites were synthesized using the sol–gel method. Bimetallic modified TiO2 nanoparticles exhibited improved photocatalytic activity under visible-light irradiation, better than monometallic Ag/TiO2 and Pt/TiO2 nanoparticles (NPs). All modified powders showed localized surface plasmon resonance (LSPR) in visible region. The photocatalysts’ characteristics by X-ray diffractometry (XRD), scanning transmission...
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Efficient production of Staphylococcus simulans lysostaphin in a benchtop bioreactor by recombinant Escherichia coli.
PublikacjaLysostaphin is an enzyme with bactericidal activity against Staphylococcus aureus and other staphylococcal species. In spite of many advantages and promising results of preliminary research, the enzyme is still not widely used in medicine, veterinary or as a food preservative. One of the most important factors limiting application of the enzyme in clinical or technological practice is the high costs of its production. In the present...
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Prozdrowotne właściwości fermentowanych napojów aroniowych i aroniowo-herbacianych
PublikacjaKombucha to lekko kwaśny, orzeźwiający napój otrzymywany w wyniku fermentacji słodkiej herbaty przez żyjące w konsorcjach bakterie octowe oraz osmofilne drożdże, tzw. grzybek herbaciany. Drobnoustroje te próbuje się wykorzystać również do produkcji funkcjonalnych napojów na bazie surowców innych niż herbata. Celem pracy było otrzymanie fermentowanych napojów z ekstraktu z wytłoków z aronii z lub bez dodatku naparu z zielonej herbaty...
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Thermal Instability of Choline Chloride-Based Deep Eutectic Solvents and Its Influence on Their Toxicity─Important Limitations of DESs as Sustainable Materials
PublikacjaDeep eutectic solvents (DESs) have become a hot topic in many branches of science due to their remarkable properties. They have been studied in a wide variety of applications. In particular, choline chloride (ChCl)-based DESs are one of the most commonly used representatives of these fluids. Nevertheless, in order to apply DESs in some fields, it is essential to guarantee their stability, reusability, and biocompatibility. In this...