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Search results for: aaph abts catechin epicatechin epigallocatechin epicatechin gallate epigallocatechin gallate hemolysis hypochlorite
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Beyond Antioxidant Activity: Redox Properties of Catechins May Affect Changes in the DNA Methylation Profile—The Example of SRXN1 Gene
PublicationThe role of catechins in the epigenetic regulation of gene expression has been widely studied; however, if and how this phenomenon relates to the redox properties of these polyphenols remains unknown. Our earlier study demonstrated that exposure of the human colon adenocarcinoma HT29 cell line to these antioxidants affects the expression of redox-related genes. In particular, treatment with (−)-epigallocatechin (EGC) downregulated...
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Role of Curcumin and (−)-Epigallocatechin-3-O-Gallate in Bladder Cancer Treatment: A Review
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Regulation of Cellular Redox Homeostasis by (–)-Epicatechin and Cocoa Extracts—A Pilot Study
PublicationCocoa polyphenols play an important role in protection against diseases in which oxidative stress is implicated as a causal or contributing factor. The main aim of this study was to elucidate the influence of different cocoa extracts and main cocoa bioactive compound (–)-epicatechin on cell growth and support of antioxidant cellular barrier in colon adenocarcinoma cell line model (HT29). Results show that the tested cocoa bioactivity...
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REGULATION OF CELLULAR REDOX HOMEOSTASIS BY (-)-EPICATECHIN AND COCOA EXTRACTS – PILOT STUDY
PublicationCocoa polyphenols play an important role in protection against diseases in which oxidative stress is implicated as a causal or contributing factor. The main aim of this study was to elucidate the influence of different cocoa extracts and main cocoa bioactive compound (–)-epicatechin on cell growth and support of antioxidant cellular barrier in colon adenocarcinoma cell line model (HT29). Results show that the tested cocoa bioactivity...
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Structure and wettability of heterogeneous monomolecular films of phospholipids with cholesterol or lauryl gallate
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Effect of chitosan on the interactions between phospholipid DOPC, cyclosporine A and lauryl gallate in the Langmuir monolayers
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Antioxidant properties of ferrous flavanol mixtures
PublicationInteraction of metal, especially iron ions with flavanols is considered as an important feature of these compounds and is believed to contribute to their both antioxidant and prooxidant properties. The aim of this study was to examine how Fe2+ binding to form a 4:1 (flavanol:Fe2+) mixtures affects the antioxidant properties of flavanols. ABTS∗ scavenging, protection against fluorescence bleaching induced by AAPH and hypochlorite,...
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Dietary antioxidants as a source of hydrogen peroxide
PublicationStudies of 54 antioxidants revealed that 27 of them, mainly polyphenols, generated hydrogen peroxide (H2O2) when added to Dulbecco’s modified Eagle’s medium (DMEM), other media used for culture of mammalian and yeast cells and phosphate-buffered saline. The most active antioxidants were: propyl gallate (PG), (−)-epigallocatechin gallate (EGCG) and quercetin (Q). Chelex treatment and iron chelators decreased H2O2 generation suggesting...
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Novel ABTS-dot-blot method for the assessment of antioxidant properties of food packaging
PublicationThe new ABTS-dot-blot method for the direct determination of antioxidant activity of active packaging that is in contact with foodstuffs has been developed. The usefulness of the new method was verified with the use of agarose, pork gelatin, bacterial cellulose and cellulose-chitosan films with incorporated standard antioxidant – Trolox or plant phytochemicals derived from three types of berry juices (chokeberry, blue-berried honeysuckle,...
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Intensification of catechin extraction from the bark of Syzygium cumini using ultrasonication: Optimization, characterization, degradation analysis and kinetic studies
PublicationCatechin is a prominent polyphenolic component that possesses various medicinal properties. Present work communicates the intensification and optimization of catechin extraction from the bark of Syzygium cumini tree using stirred reactor, soxhlet, ultrasonic bath, and ultrasonic horn technique. The optimization of several parameters such as type of solvent, solid to solvent ratio (1:100 w/v), speed of agitation (300 RPM), extraction...
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A Comparison of ABTS and DPPH methods for assessing the total antioxidant capacity of human milk
PublicationCałkowitą zdolność przeciwutleniająca (TAC) mleka ludzkiego odzwierciedla zawartość i aktywność w mleku składników, które zapobiegają degradacji tłuszczów i białek na drodze utlenienia. Celem badania było porównuje testu ABTS i DPPH w odniesieniu do odzysku, precyzji i czułości (granica wykrywalności i oznaczalności) obu metod służących do oznaczania wartości TAC mleka ludzkiego. Wartości TAC zostały określone dla dwudziestu pięciu...
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Electrochemical Production of Sodium Hypochlorite from Salty Wastewater Using a Flow-by Porous Graphite Electrode
PublicationThe production of sodium hypochlorite (NaOCl) from salty wastewater using an electrochemical cell has several advantages over other methods that often require hazardous chemicals and generate toxic waste, being more sustainable and environmentally friendly. However, the process of producing sodium hypochlorite using an electrochemical cell requires careful control of the operating conditions, such as the current density, flow rate,...
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Formation of chlorinated breakdown products during degradation of sunscreen agent, 2-ethylhexyl-4-methoxycinnamate in the presence of sodium hypochlorite
PublicationIn this study, a new degradation path of sunscreen active ingredient, 2-ethylhexyl-4-methoxycinnamate (EHMC) and 4-methoxycinnamic acid (MCA) in the presence of sodium hypochlorite (NaOCl), was discussed. The reaction products were detected using gas chromatography–mass spectrometry (GC-MS). Since HOCl treatment leads to more polar products than EHMC, application of polar extracting agents, dichloromethane and ethyl acetate/n-hexane...
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Lithium tetrakis(tri-tert-butoxysilanethiolato)gallate(III). Acta Cryst.Sect. E Struct. Rep. [online]**2002 vol. 58 s. m728-m729, 1 rys. 1 tab. bibliogr. 13 poz. Tetrakis(tri-tert-butoksysilanotiolano)gallan(III) litu.
PublicationPraca opisuje syntezę oraz strukturę krystaliczną i cząsteczkową tetrakis(tri-tert-butoksysilanotiolano)gallanu(III) litu. Kation litu jest koordynowany wewnątrz cząsteczki przez dwa atomy tlenu z dwóch grup tert-butoksylo-wych. Strukturę wyznaczono stosując rentgenowską analizę strukturalną mono-kryształu.
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The modulatory effect of green tea catechin on drug resistance in human ovarian cancer cells
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Phenolic composition and antioxidant properties of polish blue-berried honeysuckle genotypes by HPLC-DAD-MS, HPLC postcolumn derivatization with ABTS or FC, and TLC with DPPH visualization
PublicationIn this study, different Polish cultivars of blue-berried honeysuckles (Lonicera caerulea L.), wild and bog bilberry, were analyzed for bioactive compounds. The chemical properties verified included composition of anthocyanins and other polyphenols, antioxidant activity, and profiles of antioxidants by HPLC postcolumn derivatization or TLC. The antioxidant activities of different blue-berried honeysuckle cultivars were similar...
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Ultrashort Cationic Lipopeptides–Effect of N-Terminal Amino Acid and Fatty Acid Type on Antimicrobial Activity and Hemolysis
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Late subclinical hemolysis and long-term outcomes after aortic valve replacement with On-X mechanical prostheses – a preliminary single-center report
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Size Exclusion Coupled to Reversed Phase Liquid Chromatography for the Characterization of Cranberry Products
PublicationA combination of solvent extraction, size exclusion purification, and reversed-phase liquid chromatography with UV absorption and fluorescence detection was here used for the determination of flavan-3-ols and related oligomeric compounds in cranberry products. The method relied on robust, simple, and inexpensive instrumental equipment present in most of routine analytical laboratories. Sample extracts in methanol/water/hydrochloric...
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The effect of roasting beetroot and chicory on the anti-oxidative activity of their extracts
Open Research DataThe eThe data set presents the results of the assessment of the impact of roasting raw materials in the form of beetroot and chicory on their antioxidant activity measured by the standard ABTS and DPPH radical tests. The numerical results were compared with the visual assessment of the roasted raw materials. The obtained results confirmed the increase...
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Antibacterial Activity and Cytocompatibility of Bone Cement Enriched with Antibiotic, Nanosilver, and Nanocopper for Bone Regeneration
PublicationBacterial infections due to bone replacement surgeries require modifications of bone cement with antibacterial components. This study aimed to investigate whether the incorporation of gentamicin or nanometals into bone cement may reduce and to what extent bacterial growth without the loss of overall cytocompatibility and adverse effects in vitro. The bone cement Cemex was used as the base material, modified either with gentamicin...
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Post-Chromatographic Derivatization Coupled with Mass Spectrometry as a Method of Profiling and Identification of Antioxidants; Ligustrum vulgare Phytocomplex as an Example
PublicationHigh-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) coupled with radical scavenging assays, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) can be both used for the detection of the antioxidants in plant extracts. In this study, the ethanolic (70% v/v) extracts from different morphological parts of Ligustrum vulgare collected...
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Comparison of the content of phenolic compounds and antioxidant activity of white, red and black currants (Ribes sp.) extracts
PublicationThe aim of this study was to compare the content of antioxidant compounds and the antioxidant activity of extracts from white, red and black currants (Ribes sp.). The chemical properties verified included determinations of anthocyanins and other phenols by HPLC-DAD-MS, total antioxidant activity by standard spectrophotometric tests (ABTS, DPPH and FCR), and profiles of antioxidants by TLC with visualization reagents (ABTS, DPPH,...
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NUTRITIONAL AND PHARMACEUTICAL APPLICATIONS OF BIOACTIVE COMPOUNDS IN TROPICAL FRUITS
PublicationDuring the past two decades our international scientific group investigated in vitro the physicochemical and nutritional characteristics of some cultivars of durian at various stages of ripening, mangosteen and new cultivars of snake fruit in comparison with widely used avocado, kiwifruit and mango, and in vivo their influence on laboratory animals. The main objective of the present study was to screen and compare the properties...
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Application of a commercially available derivatization instrument and commonly used reagents to HPLC on-line determination of antioxidants
PublicationThis study demonstrates the potential of a commercially available derivatization instrument coupled with HPLC for separation and on-line determination of antioxidants detected with widely used screening reagents - DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) or FCR (Folin-Ciocalteu's phenol reagent ). As the result of optimization, key parameters of the analytical procedure,...
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Characterization of Bioactivity of Selective Molecules in Fruit Wines by FTIR and NMR Spectroscopies, Fluorescence and Docking Calculations
PublicationFourier transform infrared (FTIR) and proton nuclear magnetic resonance (1H NMR) spectroscopies were applied to characterize and compare the chemical shifts in the polyphenols’ regions of some fruit wines. The obtained results showed that FTIR spectra (1800–900 cm−1) and 1H NMR (δ 6.5–9.3 ppm) of different fruit wines can be used as main indices of the year of vintage and quality of fruit wines. In addition to the classical determination...
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Comparative control of the bioactivity of some frequently consumed vegetables subjected to different processing conditions
PublicationCelem prowadzonych badań było określenie wpływu warunków obróbki przed spożyciem na własciwości przeciwutleniające białej i czerwonej cebuli oraz czosnku poddanym obróbce poprzez gotowanie i blanszowanie. Do określenia poziomu aktywności przeciwutleniającej wykorzystano testy ABTS, DPPH, FRAP i CUPRAC.
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Detection of Denatonium Benzoate (Bitrex) Remnants in Noncommercial Alcoholic Beverages by Raman Spectroscopy
PublicationIllegal alcoholic beverages are often introduced into market using cheap technical alcohol, which is contaminated by denatonium benzoate (Bitrex) of very small concentration. Bitrex is the most bitter chemical compound and has to be removed before alcohol consumption. The home-made methods utilize sodium hypochlorite to disintegrate particles of denatonium benzoate in alcohol and to remove bitter taste before trading. In this experimental...
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Carbon Nanotubes Derivatized with Mediators for Laccase Catalyzed Oxygen Reduction.
PublicationPrzedstawiono konstrukcję elektrody w której wykorzystano nanorurki modyfikowane znanym mediatorem (ABTS). Cząsteczki mediatora przyłączono do ścian bocznych nanorurek oraz do ich końców. Porównano parametry elektrod zbudowanych w oparciu o nanorurki modyfikowane kowalencyjnie w różny sposób. Zaobserwowano pozytywny wpływ modyfikowanych nanorurek na parametry elektrody.
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Bioactive compounds and the antioxidant capacity in new kiwi fruit cultivars
PublicationThe aim of this investigation was to find the best among seven different kiwi fruit cultivars (‘Hayward’, ‘Daheung’, ‘Haenam’, ‘Bidan’, ‘Hort16A’, ‘Hwamei’ and ‘SKK12’) for human consumption and to classify them as groups. Therefore, the contents of bioactive compounds and the level of antioxidant capacities of these cultivars were determined in four different extracts and compared. It was found that the contents of the bioactive...
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Phytochemical composition and biological activities of differently pigmented cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis) varieties
PublicationBACKGROUND:Brassica plants contain awide spectrumofbioactive components that are responsible for their health-promoting potential such as vitamins, polyphenols and glucosinolates. This study attempted to relate the composition of bioactive phytochemicals and chosen biological activities (antioxidant, cytotoxic, anti-genotoxic, and influence on enzymatic activities) for extracts from differently pigmented cabbage (white and red)...
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Antioxidative properties of some vegetable products traditional for diets in Central European diet - short report
PublicationBadania nad przeciwutleniającymi właściwościami wybranych warzywnych produktów spożywczych prowadzone były przy użyciu testu ABTS i testu kometkowego. Wykazano, że spośród badanych produktów najwyższą aktywność przeciwutleniającą wykazywał koncentrat buraczany oraz kiszona kapusta. Sok z kiszonej kapusty w badaniach przy użyciu komórek białaczki i testu kometkowego miał zdolność ochrony komórek przed aktywnymi formami tlenu.
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Evaluating Gelatin-Based Films with Graphene Nanoparticles for Wound Healing Applications
PublicationIn this study, gelatin-based films containing graphene nanoparticles were obtained. Nanoparticles were taken from four chosen commercial graphene nanoplatelets with different surface areas, such as 150 m2/g, 300 m2/g, 500 m2/g, and 750 m2/g, obtained in different conditions. Their morphology was observed using SEM with STEM mode; porosity, Raman spectra and elemental analysis were checked; and biological properties, such as hemolysis...
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The termostability, bioactive compounds and antioxidant activity of some vegetables subjected to different durations of boiling: investigation in vitro.
PublicationThis research was performed in order to compare the water and acetone extracts of raw and boiled for 10, 20, 40 and 60 min Korean lotus roots (KLR) and Polish white onion (PWO) in the contents of theirbioactive compounds, antioxidant activity and thermostability. It was found that polyphenols (mg GAE/g), flavanols (mg GAE/g), flavonoids (mg CE/g), anthocyanins(mg CGE/kg) and tannins (mg CE/g) in water extract of raw lotus roots...
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Chemical and biological evaluation of antioxidant activity of endogenous redox-active compounds compared to plant-derived exogenous antioxidants
PublicationThe research conducted so far has shown that endogenous antioxidants, despite being regarded as the first line of antioxidant defense, may not be sufficient to maintain redox homeostasis in cells exposed to oxidative stress. The results obtained in the doctoral dissertation show that endogenous redox-active compounds were moderate or weak scavengers of ABTS and DPPH radicals, while in cellular setting, their impact on the reducing...
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The influence of different time duration of thermal processing on berries quality
PublicationOznaczano zawartość związków bioaktywnych (polifenole, flawonoidy, taniny, antocyjany i kwas askorbinowy) oraz poziom aktywności przeciwutleniającej próbek ekstraktów (wodnych, heksanowych i acetonowych) uzyskanych z różnych gatunków owoców jagodowych. Do pomiaru poziomu aktywności przeciwutleniającej wykorzystano takie testy jak ABTS, DPPH, FRAP i CUPRAC. Zbadano wpływ czasu trwania procesu obróbki termicznej na zawartość bioaktywnych...
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Changes of microbiological quality of water in distribution system. Zmiany mikrobiologicznej jakości wody w sieci dystrybucyjnej.
PublicationW artykule przedstawiono mikrobiologiczną jakość wody podziemnej uzdatnianej w dwóch stacjach oczyszczania wody (WTP I i WTP II), w sieci dystrybucyjnej. Obie stacje uzdatniania stosują dezynfekcję za pomocą dwutlenku chloru. Badania wykazały wzrost ogólnej liczby bakterii w miarę zwiększania się odległości od stacji uzdatniania.In the paper microbiological quality of water in distribution system produced in two ground water treatment...
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Partial characterization of proteins from mussel Mytilus galloprovincialis as a biomarker of contamination
PublicationPrzedmiotem zainteresowania były:- funkcjonalne i przeciwutleniające właściwości białek małża Mytilus galloprovincialis,- poziom zawartości metali ciężkich (ołów i kadm) w tkankach.Badania przeprowadzono dla małży zebranych zarówno w zanieczyszczonych, jak i w czystych obszarach strefy brzegowej Morza Czarnego w regionie Warny (Bułgaria). W badaniach wykorzystano takie techniki analityczne jak: spektroskopia FTIR, fluorescencja,...
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A comparative study of phenolic compounds and antioxidant and antiproliferative activities in frequently consumed raw vegetables
PublicationCelem badań było oszacowanie właściwości przeciwutleniających wybranych warzyw spożywczych w stanie surowym: czosnek (Allium sativum L), biała, żółta i czerwona cebula (Allium cepa L), czerwony i zielony pieprz (Capsicum annuum L.) oraz biała kapusta (Brassica oleracea var. capitata f. alba) zbieranych w ciągu tego samego roku z różnych regionów geograficznych i stref klimatycznych. W badaniach odpowiednich ekstraktów metanolowych...
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Composite Bioelectrodes Based on Lipidic Cubic Phase with Carbon Nanotube Network
PublicationElektrody z węgla szklistego modyfikowane fazą kubiczną z siecią nanorurek zastosowano do enzymatycznej redukcji tlenu katalizowanej przez lakazę. Zastosowanie nanorurek obniżyło nadpotencjał redukcji tlenu o 0,5 V. W celu zwiększenia gęstości prądu redukcji tlenu zastosowano znany mediator przeniesienia elektronów - ABTS. Mediator adsorbowano na nanorurkach lub wiązano kowalencyjnie z końcami nanorurek. Kowalencyjne związanie...
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Cu-HKUST-1 and Hydroxyapatite–The Interface of Two Worlds toward the Design of Functional Materials Dedicated to Bone Tissue Regeneration
PublicationA novel composite based on biocompatible hydroxyapatite (HA) nanoparticles and Cu-HKUST-1 (Cu-HKUST-1@HA) has been prepared following a layer-by-layer strategy. Cu-HKUST-1 was carefully selected from a group of four Cu-based metal−organic frameworks as the material with the most promising antimicrobial activity. The formation of a colloidal Cu- HKUST-1 layer on HA nanoparticles was confirmed by various techniques, e.g., infrared...
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Antioxidants and proteins in ethylene-treated kiwifruits
PublicationW pracy porównano właściwości przeciwutleniające i profil białek owoców kiwi poddanych działaniu etylenu i powietrza. Wysokie współczynniki korelacji między całkowitą zawartością fenoli i właściwościami przeciwutleniającymi uzyskano dla trzech metod określania właściwości przeciwutleniających: ABTS, TEAC i CUPRAC. W paśmie białek owoców kiwi rozdzielonych za pomocą elektroforezy stwierdzono różnice w paśmie siarczanu dodecylu sodu...
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Some analytical assays for the determination of bioactivity of exotic fruits
PublicationPrzedmiotem badań były próbki owoców egzotycznych (mango, durian, gruszka adwokacka) zebrane w 2008 roku w Tajlandii i w Izraelu (po etapie liofilizacji). W próbkach oznaczano zawartość:- białek, - tłuszczów,- węglowodanów,- flawonoidów,- tanin- polifenoli stosując zarówno zaawansowane techniki instrumentalne jak i proste testy spektrofotometryczne (FRAP, ABTS, DPPH, CUPRAC). Uzyskana wieloparametrowa baza danych pomiarowych została...
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The influence of different time durations of thermal processing on berries quality
PublicationBioactive compounds (polyphenols, flavonoids, flavanols, tannins, anthocyanins and ascorbic acid) and the level of antioxidant activity by ABTS, DPPH, FRAP and CUPRAC of water, acetone and hexane extracts of Chilean 'Murtilla' (Ugni molinae Turcz) and 'Myrteola' berries (Myrtaceae, Myrteola nummularia (Poiret) Berg.), Chilean and Polish blueberries (Vaccinium corymbosum), Chilean raspberries (Rubus idaeus), and Polish black chokeberry...
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Aktywność przeciwutleniająca polifenoli zawartych w sokach z kapusty surowej i kiszonej podczas ich obróbki termicznej.
PublicationZawartość polifenoli w soku z kapusty surowej rośnie z ~0,3mg/ml do ~12 mg/ml po 6 godz. ogrzewania, a w przypadku soku z kapusty kiszonej maleje odpowiednio z ~0,6 do ~0,14 mg/ml. Uzyskano dobrą korelację pomiędzy zawartością polifenoli a aktywnością przeciwutleniającą soków nie ogrzewanych i ogrzewanych, oznaczaną testem ABTS. Na podstawie testu β-karoten - kwas linolowy stwierdzono, że sok z kapusty surowej w całym badanym okresie...
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Comparison of bioactive compounds, antioxidant and antiproliferative activities of Mon Thong durian during ripening
PublicationCelem przeprowadzonych badań było oszacowanie zmian zawartości związków odżywczych, związków bioaktywnych, kwasów tłuszczowych oraz przeciwutleniaczy w owocach duriana w trakcie różnych etapów procesu dojrzewania. W badaniach wykorzystano testy CURRAC, DPPH, ABTS i FRAP. Stwierdzono, że całkowita zawartość polifenoli, flawonoidów, kwasu askorbimowego, tanin oraz poziom aktywności przeciwutleniającej jest dla owoców niedojrzałych,...
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Comparative characterisation of durian, mango and avocado
PublicationCelem przeprowadzonych badan była ocena właściwości odżywczych i bioaktywnych stosunkowo mało znanych egzotycznych owoców duriana i ich porównanie z parametrami charakteryzującymi powszechnie znane owoce mango i gruszki adwokackiej. W badaniach wykorzystano takie techniki jak HPLC, trójwymiarowa fluorescencja (3D-FL), testy oparte na zastosowaniu rodników (ABTS i DPPH) oraz zaawansowane techniki chemometryczne. na podstawie uzyskanych...
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The antioxidant properties of anthocyanins in different Aronia products
PublicationOgromne znaczenie w patologii wielu chorób, w tym chorób układu krążenia oraz nowotworów, ma stan przewlekłego stresu oksydacyjnego. Podwyższonej produkcji reaktywnych form tlenu mogą zapobiegać naturalne przeciwutleniacze pochodzenia roślinnego. Z tego powodu przedmiotem badań w wielu ośrodkach naukowych na świecie są rośliny, których składniki charakteryzują się wysokim potencjałem przeciwutleniającym. Do takich roślin zalicza...
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The thermostability, bioactive compounds and antioxidant activity of some vegetables subjected to different durations of boiling: investigation in vitro
PublicationThis research was performed in order to compare the water and acetone extracts of raw and boiled for 10, 20, 40 and 60 min Korean lotus roots (KLR) and Polish white onion (PWO) in the contents of theirbioactive compounds, antioxidant activity and thermostability.It was found that polyphenols (mg GAE/g), flavanols (mg GAE/g), flavonoids (mg CE/g), anthocyanins (mg CGE/kg) and tannins (mg CE/g) in water extract of raw lotus roots...
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Partial characterization of white cabbages (Brassica oleracea var. capitata f. alba) from different regions by glucosinolates, bioactive compounds, total antioxidant activities and proteins
PublicationW pracy przedstawiono wyniki oznaczeń glukozynolanów (GLS), aktywności przeciwutleniającej (TRSA) oraz białek w próbkach białej kapusty pochodzącej z różnych rejonów geograficznych Europy. Glukobrassycyna i sinigryna były dominującymi GLS we wszystkich próbkach kapusty. Całkowita zawartość GLS mieściła się w zakresie od 3,3-7,7 µmol/g sm. Aktywność przeciwulteniająca próbek kapusty została oszacowana za pomocą testów ABTS,...
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COMPOSITION OF BIOACTIVE SECONDARY METABOLITES AND MUTAGENICITY OF SAMBUCUS NIGRA L. FRUIT AT DIFFERENT STAGES OF RIPENESS
PublicationThe relationship between the content of bioactive compounds and mutagenic activity of elderberry fruit at different stages of ripeness was investigated. Signifi cant differences in the antioxidant profi les (TLC, HPLC with post-column derivatization) and antioxidant activity (ABTS, DPPH, and FC tests) were observed for studied elderberry extracts. The more ripen the fruit at the time of harvest were, the higher the content of...
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The effect of heating and fermenting on antioxidant properties of white cabbage
PublicationIt is widely believed that natural antioxidants found in food are significantly lost during processing. Nevertheless, it was recently demonstrated that processed fruits and vegetables may retain their antioxidant activity. In the present work, the changes in the overall antioxidant properties as a consequence of fermentation of cabbage and/or heat treatment of cabbage juices and extracts were studied. Fermentation processes as...
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Partial characterization of three Korean white lotus cultivars
PublicationThe nutritional and bioactive values of Korean white lotus cultivars (Muan, Chungyang, and Garam)extracted with methanol, water, and acetone solvents were evaluated by the contents of their bioactive compounds and antioxidant activity determined by ABTS, CUPRAC, FRAP, DPPH, and β-carotene-linoleic acid assays. HPLC, fluorometry, and FTIR spectroscopy were employed for evaluation of polyphenols and fatty acids. All lotus cultivar...
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Antioxidant properties and bioactive constituents of some rare exotic thai fruits and comparison with conventional fruits. in vitro and in vivo studies
PublicationPrzedmiotem badań były ekstrakty uzyskane zarówno z egzotycznych owoców z terenu Tajlandii (durian, snake fruit, mangosteen) jak i owoców ''konwencjonalnych'' (mango, kiwi, awokado) w celu porównania zawartości związków bioaktywnych i aktywności przeciwutleniającej.Wyniki badań prowadzonych zarówno in vitro jak i in vivo mogą być wykorzystane przy opracowywaniu diety. W trakcie badań in vitro wykorzystano testy do określania potencjału...
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Chicken feather keratin as a source of bioactive peptides useful in prevention of metabolic disorders – in silico and in vitro hydrolysis
PublicationProtein derived bioactive peptides not only serve as nutrients but can also exert drug-like activity, e.g. antidiabetic, antihypertensive, or antimicrobial, to name a few. Most biopeptides have beneficial effects on health that make them attractive for nutraceutical applications. The majority of bioactive peptides have been obtained from food proteins, but protein-rich by-products generated by agriculture...
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Properties of Different Varieties of Durian
PublicationDurian (Durio zibethinus Murr.), like many other exotic, tropical, and conventional fruits, is important in the prevention of different diseases. In this study, the characterization of the main bioactive compounds of the most popular cultivars of durian and their properties are described. The changes in the quality indices of the antioxidant status were determined by CUPRAC, ABTS, FRAP, DPPH, and ORAC assays. The profiling of phytochemicals...
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Carbon nanotubes chemically derivatized with rebox systems as mediators for biofuel cell applications
PublicationEnzymatyczne bioogniwa paliwowe to urządzenia wykorzystujące enzymy do przemiany energii chemicznej w elektryczną. Zyskana w ten sposób energia może służyć do zasilania wszczepialnych urządzeń (np. czujników glukozy, rozruszników serca).Przedstawiono sposób otrzymywania i właściwości bioelektrod, w których wykorzystano nanorurki węglowe modyfikowane mediatorami enzymatycznych reakcji redoks. Do nanorurek przyłączano zarówno mediatory...
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Carbon Nanotubes Chemically Derivatize with Redox Systems as Mediators for Biofel Cell Applicatoions
PublicationEnzymatyczne bioogniwa paliwowe to urządzenia wykorzystujące enzymy do przemiany energii chemicznej w elektryczną. Zyskana w ten sposób energia może służyć do zasilania wszczepialnych urządzeń (np. czujników glukozy, rozruszników serca).Przedstawiono sposób otrzymywania i właściwości bioelektrod, w których wykorzystano nanorurki węglowe modyfikowane mediatorami enzymatycznych reakcji redoks. Do nanorurek przyłączano zarówno mediatory...
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Carbon Nanotubes Chemically Derivatized with Redox Systems as Mediators for Biofuel Cell Applications
PublicationEnzymatyczne bioogniwa paliwowe to urządzenia wykorzystujące enzymy do przemiany energii chemicznej w elektryczną. Zyskana w ten sposób energia może służyć do zasilania wszczepialnych urządzeń (np. czujników glukozy, rozruszników serca). Przedstawiono sposób otrzymywania i właściwości bioelektrod, w których wykorzystano nanorurki węglowe modyfikowane mediatorami enzymatycznych reakcji redoks. Do nanorurek przyłączano zarówno mediatory...
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Preparation and characterization of genipin cross-linked porous chitosan–collagen–gelatin scaffolds using chitosan–CO2 solution
PublicationNovel porous scaffolds composed of chitosan, collagen and gelatin were prepared by the multistep procedure involving final freeze-drying and characterized. To eliminate the need for residual acid removal from the material after drying, carbon dioxide saturation process was used for chitosan blend formulation. The use of CO2 for chitosan dissolution made the scaffold preparation process more reproducible and economically sustainable....
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The relationship between standard reduction potentials of catechins and biological activities involved in redox control
PublicationRedox homeostasis involves factors that ensure proper function of cells. The excess reactive oxygen species (ROS) leads to oxidative stress and increased risk of oxidative damage to cellular components. In contrast, upon reductive stress, insufficient ROS abundance may result in faulty cell signalling. It may be expected that dietary antioxidants, depending on their standard reduction potentials (E°), will affect both scenarios....
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Effects of Salvia officinalis and Thymus vulgaris on oxidant-induced DNA damage and antioxidant status in HepG2 cells
PublicationSalvia officinalis (SO) and Thymus vulgaris (TV) are medicinal plants well known for their curative powers. However, the molecular mechanisms responsible for these abilities of sage and thyme have not been fully understood yet. In this study we investigated the composition and the quantitative estimation of plant extracts, the protective effects of plant extracts against hydrogen peroxide- and 2,3-dimethoxy-1,4 naphthoquinone-induced...
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Uncertainty of antioxidant profiling in complex mixtures using liquid chromatography involving post-column derivatisation
PublicationThe main goal of this paper is to discuss the problems associated with antioxidant profiling in complex samples using a high-throughput HPLC system coupled with post-column derivatisation reactor. Based on the experimental data reported in the literature, we demonstrated that improper optimisation of temperature and/or pH assay conditions performed using an on-line derivatisation reactor may substantially change the antioxidant...
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Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage
PublicationExposure to high levels of different environmental pollutants is known to be associated with induction of DNA damage in humans. Thus DNA repair is of great importance in preventing mutations and contributes crucially to the prevention of cancer. In our study we have focused on quantitative analysis of Gentiana asclepiadea aqueous or methanolic extracts obtained from flower and haulm, their antioxidant potency in ABTS post-column...
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Biological Activity of Tea-Chokeberry and Chokeberry Fermented Beverages
PublicationFermented beverages, such as kombucha are gaining more and more popularity due to their beneficial effects on human health. These products are obtained by the fermentation of tea infusion using so called tea fungus. This microbial consortium can also be used to prepare fermented fruit beverages. Therefore, the aim of this study was to determine the changes in the antioxidant profile of tea-chokeberry and chokeberry beverages...
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Interaction of human serum albumin with volatiles and polyphenols from some berries
PublicationHeadspace solid-phase microextraction coupled with comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (HS-SPME/GC GCeTOFMS) identified 19 and quantified 6 monoterpenes in gooseberries, blueberries and cranberries. As a protein model human serum albumin (HSA) was used in interaction with terpenes. Specific binding of terpenes to HSA under the physiological conditions was a result of the formation...
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Anticarcinogenic and antioxidant activities of leaves and flowers hydroalcoholic extracts of Nerium oleander L.: PCA analysis and phytochemical content by FTIR spectroscopy
PublicationAims: The aim of this work is the evaluation the anticarcinogenic effect on HT29 cancer cells lines and antioxidant effect of three extracts from different morphological parts (leaves (LE), pink flowers (PFE) and white flowers (WFE)) of Nerium oleander lin. Methods: This research provides the anticarcinogenic activity of extracts from N. oleander white flowers. Principal Component Analysis (PCA) was applied to estimate the relationship...
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Young Shoots of White and Red Headed Cabbages Like Novel Sources of Glucosinolates as Well as Antioxidative Substances
PublicationMost literature data indicate that the diet rich in plant products reduces the risk of developing chronic non-communicable diseases and cancer. Brassica vegetables are almost exclusively synthesizing glucosinolates. Glucosinolates are higher in sprouts than in mature plants, being related to the activity of the specific myrosinase involved in the degradation of glucosinolates during developmental stages. This study compares the...
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The influence of plant protection by effective microorganisms on the content of bioactive phytochemicals in apples
PublicationThe phytochemicals of two apple cultivars (Yellow Transparent and Early Geneva) protected in two ways, conventionally with chemical pesticides or by effective microorganisms (EM), were compared. Two types of components were determined: lipids synthesised constitutively and generated via inducible pathways polyphenols along with antioxidant activity and profiles. The antioxidant activities assessed with ABTS, DPPH and Folin–Ciocalteu...
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Comparative Study on Assisted Solvent Extraction Techniques for the Extraction of Biologically Active Compounds from Sideritis raeseri and Sideritis scardica
PublicationThe plants in the Sideritis genus are postulated to exhibit several important medicinal properties due to their unique chemical composition. To isolate the targeted phytochemical compounds, the selection of a suitable extraction method is of primary importance. In this work, a comparative study on the phytochemical profiles of various Sideritis raeseri and Sideritis scardica extracts has been carried out. An untargeted metabolomics...
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Bioactivities of Phenolic Compounds from Kiwifruit and Persimmon
PublicationFruit used in the common human diet in general, and kiwifruit and persimmon particularly, displays health properties in the prevention of heart disease. This study describes a combination of bioactivity, multivariate data analyses and fluorescence measurements for the differentiating of kiwifruit and persimmon, their quenching and antioxidant properties. The metabolic differences are shown, as well in the results of bioactivities...
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Comparative assessment of two extraction procedures for determination of bioactive compounds in some berries used for daily food consumption
PublicationTwo extractions with methanol and water were used to determine the antioxidant and binding properties of some berries as a supplement to food. Fluorometry, FTIR spectra and radical scavenging assays were used for characterisation of bioactive compounds (polyphenols, flavonoids, flavanols and tannins) and the levels of their antioxidant activities (AAs). The contents of bioactive compounds and AAs in water and methanol polyphenol...
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Juices from non-typical edible fruits as health-promoting acidity regulators for food industry
PublicationThe study verifies the possibility of application of juices from selected fruits characterized by the high antioxidant potential as natural acidity regulators with improved nutritional properties. The tested non-typical fruits included mirabelle plum, sea buckthorn and blue-berried honeysuckle. Beetroot juice whose pH is about 6.0 served as a model food product. Potentiometric titration was used to compare the efficacy of tested...
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Characterization of white cabbages from different cultivations by isothiocynates content and antioxidant activities
PublicationBadania epidemiologiczne wskazują, że wraz z zwiększeniem spożywania kapusty maleje ryzyko zachorowania na niektóre nowotwory. Mechanizmy przeciwrakotwórczego działania nie są do końca wyjaśnione, jednak wiele badań przeprowadzonych z wykorzystaniem zwierząt i ludzi sugeruje, że substancje występujące w tych roślinach, szczególnie izotiocyjaniany (ITC) modulują aktywność enzymów I i II fazy. Aktywność przeciwutleniająca jest innym...
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Antioxidant activities and bioactive components in some berries
PublicationThe objective of this study was to evaluate the antioxidant and binding effects of gooseberry, a less-stud-ied berry, and to compare with blueberry and cranberry in the model of interaction with human serum albumin (HSA). The relationship between the scavenging properties of dietary polyphenols of the selected berries and their affinities for HSA were investigated by fluorescence anal- ysis. In order to perform the extraction and...
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The influence of selenium addition during seeds' germination on the biological properties of obtained sprouts
PublicationSelenium plays a vital role in human body, because in the form of aminoacids (Se-methionine, Se-cysteine) it is found in the active centres of over 25 enzymes, including those regarded as 'cytoprotective'. Unfortunatelly, its daily intake in Poland and other European countries is lower than predicted by dietary recommendations. The consequences of selenium deficiency in diet might be severe, including higher susceptibility to cardio-vascular...
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The optimisation of analytical parameters for routine profiling of antioxidants in complex mixture by HPLC coupled post-column derivatisation
PublicationIntroduction: The wide application of natural and artificial antioxidants in food, cosmetic and pharmaceutical industry as well as the recognition of the importance of food antioxidants for supporting human health created demand for reliable and industrially applicable methods of determining antioxidative activity. This requirement can be fullfilled with the recently proposed HPLC-post-column derivatisation approach enabling the...
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Structure, Physicochemical and Biological Properties of an Aqua (2,2′,2′′-Nitrilotriacetato)-oxidovanadium(IV) Salt with 4-Methylpyridinium Cation
PublicationThe crystal structure of a nitrilotriacetate (nta) oxidovanadi-um(IV) salt with 4-methylpyridinium cation, [4-Me(Py)H]+, of [4-Me(Py)H][VO(nta)(H2O)] stoichiometry was determined. The com-plex comprises a discrete mononuclear [VO(nta)(H2O)]–coordinationentity that can be rarely found among other known compounds con-taining nitrilotriacetate oxidovanadium(IV) moieties. The complex wascharacterized by spectroscopic (IR and EPR) methods,...
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Relationship between Chemical Structure and Biological Activity Evaluated In Vitro for Six Anthocyanidins Most Commonly Occurring in Edible Plants
PublicationNumerous studies have provided evidence that diets rich in anthocyanins show a broad spectrum of health benefits. Anthocyanins in nature are usually found in the form of glycosides. Their aglycone forms are called anthocyanidins. The chemical structure of anthocyanins is based on the flavylium cation, but they differ in the position and number of substituents. However, the bioactives and foods that contain them are frequently treated...
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Detection of Bioactive Compounds in Organically and Conventionally Grown Asparagus Spears
PublicationIn the recent reports, there are contradictory conclusions about the nutritional and health properties of organic and conventionally growing vegetables. We hypothesized that organic cultivation system results in higher quality of asparagus (Asparagus officinalis L.) because of organic manure and effective organisms. Therefore, new analytical methods were applied in order to find the differences in bioactive compounds between the...
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Comparative Analysis of Metabolic Variations, Antioxidant Profiles and Antimicrobial Activity of Salvia hispanica (Chia) Seed, Sprout, Leaf, Flower, Root and Herb Extracts
PublicationThe purpose of this study was to evaluate the phytochemical profiles of the seeds, sprouts, leaves, flowers, roots and herb of Salvia hispanica and to demonstrate their significant contribution to antioxidant and antimicrobial activities. Applied methods were: HPLC-DAD coupled with post-column derivatization with ABTS reagent, untargeted metabolomics performed by LC-Q-Orbitrap HRMS, and two-fold micro-dilution broth method, which...
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Influence of steam cooking on pro-health properties of Small and Large variety of Momordica charantia
PublicationSteam cooking is one of the most common preparations of Momordicacharantia dishes. This method improved the tastiness of the fruits but at the same time, some changes occurred in the volatile and non-volatile parts of their food matrices. In this study, for the first time these properties were correlated with the found substances affecting the bioactivity of this fruit. Two varieties of Momordicacharantia were analysed and compared....
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Nutritional and Pharmaceutical Properties of Bioactive Compounds in Organic and Conventional Growing Kiwifruit
PublicationThe bioactivity of two kiwifruit’s cultivars growing under organic and conventional conditions were studied and compared. The bioactive compounds were extracted with water and ethanol using similar conditions which are applied in pharmaceutical applications and for daily fruit consumption such as tea drink. Antioxidant radical scavenging assays [ferric-reducing/antioxidant power (FRAP); cupric reducing antioxidant capacity (CUPRAC);...
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Application of analytical methods for the determination of bioactive compounds in some berries
PublicationFluorometry, ESI-MS, FTIR, and radical scavenging assays were used for characterization of bioactive compounds and the levels of their antioxidant activities. Polyphenols, flavonoids, anthocyanins, and ascorbic acid and the level of antioxidant activity of water extracts of “Murtilla-like” [Myrteola nummularia (Poiret) Berg.], and other widely consumed berries were determined and compared. The contents of bioactive compounds and...
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Antioxidant and antimicrobial activity of fermented beverages obtained from fruit pomace
PublicationFruit pomace should not be considered as a waste product but as a by-product as it contains a lot of valuable components such as dietary fiber, bioactive compounds and a source of nutrients. The reasonable way to utilize these by-products, both fresh or dried, could be the fermentation of its extracts, leading to beverages with functional properties. In our research, we checked the possibility of using chokeberry, apple and...
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Relationship between the betalain composition in Opuntia ficus indica and Beta vulgaris varieties and biological activity of their extracts
PublicationBetalains are a class of pigments that are much less com- monly found in nature than anthocyanins or carotenoids. They occur only in a few edible plants such as red beetroot (Beta vulgaris), prickly pears (Opuntia ficus indica), amaranth (Amaranthus sp.) and some mushrooms. This group of pig- ments can be divided into two major groups: yellow vulgax- anthins and red betanins. Despite their structural similarity to compounds from...
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Juices from untypical edible fruits as acidity regulators for food industry. Verification of health promoting properties. Comparison with typical acidity regulators.
PublicationNowadays all kind of food additives, and among them acidity regulators, have become necessary components of most food products. Their role is lengthening shelf-life, improving the sensory properties and protecting food products from microbial contaminations. However, despite advantages, there are also some undesirable effects of use of food additives that have raised public concern recently. Therefore, food industry considers the...
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Partial characterization of indigo (Polygonum tinctorium Ait.) plant seeds and leaves
PublicationThe aim of this study was to assess the contents of indigo's bioactive compounds, its antioxidant and anticancer activities in acetone, hexane and DMSO extracts and to compare the overall bioactivity with another more used medicinal plant named prolipid. It was found that the contents of the bioactive compounds in the studied extracts from different parts of indigo plant varied (P < 0.05): the significantly highest content of polyphenols...
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The impact of cooking method on the phenolic composition, total antioxidant activity and starch digestibility of rice (Oryza sativa L.)
PublicationThis study investigated changes in the phenolic composition, total antioxidant activity (TAA) and starch digestibility in white and brown rice due to three different cooking procedures, and subsequent reheating of cooked rice after storage. Among the analyzed samples, brown rice showed the highest TAA and phenolic content (622.5 mg kg-1 DW). All cooking methods resulted in significant decrease of phenolic content and TAA of rice...
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Quality of limes juices based on the aroma and antioxidant properties
PublicationKaffir (Citrus hystrix) and Key (Citrus aurantifolia) limes juices were investigated and compared. Two dimensional gas chromatography coupled with time-of-flight mass spectrometry (GC×GC-TOF-MS) was applied to assess the botanical origin of Kaffir and Key limes juices, based on volatile substances. The biggest differences in the contents of selected terpenes in Kaffir and Key limes occur in chemical compounds such as Limonene,...
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Metody oznaczania właściwości przeciwutleniających fitozwiązków w systemach komórkowych z wykorzystaniem zjawiska fluorescencji/luminescencji.
PublicationOd czasu odkrycia roli reaktywnych form tlenu (RFT) w etiologii tzw. chorób cywilizacyjnych, obejmujących nieinfekcyjne chroniczne choroby, takie jak nowotwory, cukrzyca czy nadciśnienie tętnicze oraz możliwości wykorzystania związków o charakterze przeciwutleniaczy w walce z tymi chorobami, naukowcy opracowali wiele metod służących do oznaczania aktywności przeciwutleniającej , zarówno czystych związków chemicznych, jak i ekstraktów...
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Hypericum alpestre extract exhibits in vitro and in vivo anticancer properties by regulating the cellular antioxidant system and metabolic pathway of L‐arginine
PublicationConventional treatment methods are not effective enough to fight the rapid increase in cancer cases. The interest is increasing in the investigation of herbal sources for the development of new anticancer therapeutics. This study aims to investigate the antitumor capacity of Hypericum alpestre (H. alpestre) extract in vitro and in vivo, either alone or in combination with the inhibitors of the L‐arginine/polyamine/nitric oxide...
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Selectivity Tuning by Natural Deep Eutectic Solvents (NADESs) for Extraction of Bioactive Compounds from Cytinus hypocistis—Studies of Antioxidative, Enzyme-Inhibitive Properties and LC-MS Profiles
PublicationIn the present study, the extracts of Cytinus hypocistis (L.) L using both traditional solvents (hexane, ethyl acetate, dichloromethane, ethanol, ethanol/water, and water) and natural deep eutectic solvents (NADESs) were investigated in terms of their total polyphenolic contents and antioxidant and enzyme-inhibitive properties. The extracts were found to possess total phenolic and total flavonoid contents in the ranges of 26.47–186.13...
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Blue-berried honeysuckle a promising cancer preventing fruit; Polish genotypes as a source of bioactive phytochemicals
PublicationFrom the ancient times, plants containing health-oriented compounds in their matrices are used in the treatment of different complaints and diseases. One of these groups are plant secondary metabolites (PSM), especially phenolic compounds, terpenes and terpenoids. A large number of PSM exhibit cytotoxicity against a variety of tumor cells, as well as cancer preventive and anticancer efficacy in preclinical animal models. For this...
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The Action Mechanisms, Anti-Cancer and Antibiotic-Modulation Potential of Vaccinium myrtillus L. Extract
PublicationHerbal medicinal products containing Vaccinium myrtillus L. (bilberry) fruits and fruit extracts are widely available in the market. Although bilberry leaves and stems are considered as bio-waste, they contain much higher levels of phenolic compounds than fruits. The study aimed to investigate the antimicrobial and anticancer potential of aerial part extracts from Vaccinium myrtillus L. (V. myrtillus, VM) plants harvested at high...
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The influence of fresh cabbage and sauerkraut juices on the activity of protective enzymes in human cell lines
PublicationMechanizmami przeciwrakotwórczego działania związków zawartych w kapuście jest m.in. metaboliczna aktywacja enzymów II fazy detoksykacji, indukcja enzymów naprawczych DNA oraz aktywnośc przeciwutleniająca. Do enzymów II fazy odtruwania należą m.in. S-transferazy glutationowe (GST) oraz oksydoreduktaza chinonowa NQO1 zwana też DT-diaforazą. GST katalizują reakcję sprzęgania glutationu z wieloma różnymi typami ksenobiotyków, ułatwiając...